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P-glycoprotein (ABCB1) is involved in vitamin K efflux
- Source :
- Food Chemistry, Food Chemistry, 2020, 343, pp.1-5. ⟨10.1016/j.foodchem.2020.128510⟩, Food Chemistry, Elsevier, 2020, 343, ⟨10.1016/j.foodchem.2020.128510⟩, Food Chemistry, Elsevier, 2020, 343, pp.1-5. ⟨10.1016/j.foodchem.2020.128510⟩
- Publication Year :
- 2020
- Publisher :
- HAL CCSD, 2020.
-
Abstract
- International audience; ABCB1 (P-glycoprotein/MDR1) is a multidrug efflux transporter that has previously been involved in cholesterol and vitamin D metabolism. Our aim was to explore whether ABCB1 is also involved in vitamin K efflux. Vitamin K apical efflux was significantly decreased in presence of ABCB1 inhibitor in Caco-2 cells (− 20.4%; p < 0.05) and increased in Griptite cells overexpressing ABCB1 (+40.7%; p < 0.05). In vivo, the vitamin K postprandial response was higher in male Abcb1 − /− mice after gavage compared to control animals (+115%; p < 0.05), but was unchanged in female mice. Finally, a vitamin K transintestinal efflux and a biliary vitamin K efflux were observed, but the specific involvement of ABCB1 could not be confirmed in these pathways. Overall, we showed for the first time that ABCB1 is involved in enterocyte vitamin K efflux in both cell and mouse models and regulates vitamin K absorption in mice. Recently, the multidrug membrane transporter ABCB1 (Sharom, 2011) has been identified to contribute to the intestinal transport of vitamin D (Margier et al., 2019
- Subjects :
- Male
Vitamin K
ATP-binding cassette B1
Bioavailability
030309 nutrition & dietetics
[SDV.MHEP.PHY] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]
NPC1L1
Vitamin k
Analytical Chemistry
chemistry.chemical_compound
scavenger receptor class B type I
Bile
PBS
[SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
0303 health sciences
Chemistry
SR-BI
General Medicine
[SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
Postprandial Period
Recombinant Proteins
Intestine
[SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system
medicine.anatomical_structure
P glycoprotein abcb1
Enterocyte
Female
Efflux
medicine.medical_specialty
DMEM
Phylloquinone MDR1 Bioavailability Intestine Enterocyte Bile ABCB1
MDR1
phosphate-buffered saline
NPC1 transporter like 1
03 medical and health sciences
[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system
Internal medicine
Phylloquinone
medicine
[SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]
Animals
Humans
ATP Binding Cassette Transporter, Subfamily B, Member 1
Dulbecco's Modified Eagle's
030304 developmental biology
Vitamin D metabolism
Cholesterol
CD36 molecule
Transporter
[SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
Mice, Mutant Strains
[SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
Enterocytes
Endocrinology
Caco-2 Cells
CD36
Food Science
Subjects
Details
- Language :
- English
- ISSN :
- 03088146 and 18737072
- Database :
- OpenAIRE
- Journal :
- Food Chemistry, Food Chemistry, 2020, 343, pp.1-5. ⟨10.1016/j.foodchem.2020.128510⟩, Food Chemistry, Elsevier, 2020, 343, ⟨10.1016/j.foodchem.2020.128510⟩, Food Chemistry, Elsevier, 2020, 343, pp.1-5. ⟨10.1016/j.foodchem.2020.128510⟩
- Accession number :
- edsair.doi.dedup.....6680ab11dce88d5fea9a9998989f3b97