Search

Your search keyword '"Doris M. Bravo Garcia"' showing total 2 results
Start Over Author "Doris M. Bravo Garcia"
2 results on '"Doris M. Bravo Garcia"'

1. Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results

Author

W. Ray Waters, H. Martin Vordermeier, Shelley Rhodes, Bhagwati Khatri, Mitchell V. Palmer, Mayara F. Maggioli, Tyler C. Thacker, Jeffrey T. Nelson, Bruce V. Thomsen, Suelee Robbe-Austerman, Doris M. Bravo Garcia, Mark A. Schoenbaum, Mark S. Camacho, Jean S. Ray, Javan Esfandiari, Paul Lambotte, Rena Greenwald, Adrian Grandison, Alina Sikar-Gang, and Konstantin P. Lyashchenko

Subjects
Antibody, Bovine tuberculosis, Dual path platform, Multi-antigen print immunoassay, Tuberculin skin test, Mycobacterium bovis, Veterinary medicine, SF600-1100
Abstract

Abstract Background Bovine tuberculosis (TB) control programs generally rely on the tuberculin skin test (TST) for ante-mortem detection of Mycobacterium bovis-infected cattle. Results Present findings demonstrate that a rapid antibody test based on Dual-Path Platform (DPP®) technology, when applied 1-3 weeks after TST, detected 9 of 11 and 34 of 52 TST non-reactive yet M. bovis-infected cattle from the US and GB, respectively. The specificity of the assay ranged from 98.9% (n = 92, US) to 96.0% (n = 50, GB) with samples from TB-free herds. Multi-antigen print immunoassay (MAPIA) revealed the presence of antibodies to multiple antigens of M. bovis in sera from TST non-reactors diagnosed with TB. Conclusions Thus, use of serologic assays in series with TST can identify a significant number of TST non-reactive tuberculous cattle for more efficient removal from TB-affected herds.

Published
2017
Full Text
View/download PDF

2. Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results

Author

Konstantin P. Lyashchenko, Javan Esfandiari, Mark A. Schoenbaum, Shelley G. Rhodes, Bruce V. Thomsen, Rena Greenwald, Mitchell V. Palmer, Bhagwati Khatri, Paul Lambotte, Suelee Robbe-Austerman, Alina Sikar-Gang, H. Martin Vordermeier, Jean S. Ray, Tyler C. Thacker, Jeffrey T. Nelson, Mayara F. Maggioli, Doris M. Bravo Garcia, Adrian Grandison, W. Ray Waters, and Mark S. Camacho

Subjects
0301 basic medicine, Male, Time Factors, Dual path platform, Multi-antigen print immunoassay, 040301 veterinary sciences, Tuberculin, Enzyme-Linked Immunosorbent Assay, Immunoglobulin G, Serology, Bovine tuberculosis, 0403 veterinary science, 03 medical and health sciences, Antigen, medicine, Animals, Antibody, Mycobacterium bovis, lcsh:Veterinary medicine, General Veterinary, medicine.diagnostic_test, biology, Tuberculin skin test, business.industry, Tuberculin Test, 04 agricultural and veterinary sciences, General Medicine, biology.organism_classification, bacterial infections and mycoses, Virology, Antibodies, Bacterial, 030104 developmental biology, Immunoassay, Immunology, biology.protein, lcsh:SF600-1100, Cattle, Female, business, Tuberculosis, Bovine, Mycobacterium, Research Article
Abstract

Background Bovine tuberculosis (TB) control programs generally rely on the tuberculin skin test (TST) for ante-mortem detection of Mycobacterium bovis-infected cattle. Results Present findings demonstrate that a rapid antibody test based on Dual-Path Platform (DPP®) technology, when applied 1-3 weeks after TST, detected 9 of 11 and 34 of 52 TST non-reactive yet M. bovis-infected cattle from the US and GB, respectively. The specificity of the assay ranged from 98.9% (n = 92, US) to 96.0% (n = 50, GB) with samples from TB-free herds. Multi-antigen print immunoassay (MAPIA) revealed the presence of antibodies to multiple antigens of M. bovis in sera from TST non-reactors diagnosed with TB. Conclusions Thus, use of serologic assays in series with TST can identify a significant number of TST non-reactive tuberculous cattle for more efficient removal from TB-affected herds.

Published
2016

Catalog

Books, media, physical & digital resources
See catalog results