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Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results

Authors :
W. Ray Waters
H. Martin Vordermeier
Shelley Rhodes
Bhagwati Khatri
Mitchell V. Palmer
Mayara F. Maggioli
Tyler C. Thacker
Jeffrey T. Nelson
Bruce V. Thomsen
Suelee Robbe-Austerman
Doris M. Bravo Garcia
Mark A. Schoenbaum
Mark S. Camacho
Jean S. Ray
Javan Esfandiari
Paul Lambotte
Rena Greenwald
Adrian Grandison
Alina Sikar-Gang
Konstantin P. Lyashchenko
Source :
BMC Veterinary Research, Vol 13, Iss 1, Pp 1-7 (2017)
Publication Year :
2017
Publisher :
BMC, 2017.

Abstract

Abstract Background Bovine tuberculosis (TB) control programs generally rely on the tuberculin skin test (TST) for ante-mortem detection of Mycobacterium bovis-infected cattle. Results Present findings demonstrate that a rapid antibody test based on Dual-Path Platform (DPP®) technology, when applied 1-3 weeks after TST, detected 9 of 11 and 34 of 52 TST non-reactive yet M. bovis-infected cattle from the US and GB, respectively. The specificity of the assay ranged from 98.9% (n = 92, US) to 96.0% (n = 50, GB) with samples from TB-free herds. Multi-antigen print immunoassay (MAPIA) revealed the presence of antibodies to multiple antigens of M. bovis in sera from TST non-reactors diagnosed with TB. Conclusions Thus, use of serologic assays in series with TST can identify a significant number of TST non-reactive tuberculous cattle for more efficient removal from TB-affected herds.

Details

Language :
English
ISSN :
17466148
Volume :
13
Issue :
1
Database :
Directory of Open Access Journals
Journal :
BMC Veterinary Research
Publication Type :
Academic Journal
Accession number :
edsdoj.80dfc14de84848ac8eadf2f377755d83
Document Type :
article
Full Text :
https://doi.org/10.1186/s12917-017-1085-5