1. Methylation of the L1 gene and integration of human papillomavirus 16 and 18 in cervical carcinoma and premalignant lesions
- Author
-
Francisco I Torres-Rojas, Julio Ortiz-Ortiz, Eugenia Flores-Alfaro, Dinorah Leyva-Illades, Oscar Del Moral-Hernández, Hugo Alberto Rodríguez-Ruiz, Daniel Hernández-Sotelo, Berenice Illades-Aguiar, Luz del Carmen Alarcón-Romero, Marco Antonio Leyva-Vázquez, and Miguel A Mendoza-Catalán
- Subjects
0301 basic medicine ,Cancer Research ,cervical cancer ,integration ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Epigenetics ,squamous intraepithelial lesion ,Cervical cancer ,DNA methylation ,business.industry ,biomarkers ,virus diseases ,Cancer ,Articles ,Methylation ,medicine.disease ,human papillomavirus 18 ,Squamous intraepithelial lesion ,human papillomavirus 16 ,030104 developmental biology ,Real-time polymerase chain reaction ,Oncology ,CpG site ,030220 oncology & carcinogenesis ,Cancer research ,L1 gene ,business - Abstract
High-risk human papillomavirus (HPV) is the primary cause of cervical carcinoma (CC). Viral integration into the host chromosomes is associated with neoplastic progression, and epigenetic changes may occur as a result. The objective of the present study was to analyze HPV L1 gene methylation and to compare the use of quantitative polymerase chain reaction (qPCR), in situ hybridization (ISH) and L1 methylation analysis as methods for detecting HPV integration. Cervical scrapes or biopsy samples positive for HPV 16 or 18, from 187 female patients with CC, squamous intraepithelial lesions (SILs) or no intraepithelial lesion (non-IL) were analyzed. Methylation of the L1 gene was determined using bisulfite modification followed by PCR, and HPV integration was subsequently analyzed. HPV 16 L1 gene methylation was revealed to increase with histological grade, with statistically significant differences observed as follows: Low-grade SIL vs. CC, P
- Published
- 2017