8 results on '"Dingliang Zhang"'
Search Results
2. The MAPK-Alfin-like 7 module negatively regulates ROS scavenging genes to promote NLR-mediated immunity
- Author
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Dingliang Zhang, Zongyu Gao, He Zhang, Yizhou Yang, Xinxin Yang, Xiaofei Zhao, Hailong Guo, Ugrappa Nagalakshmi, Dawei Li, Savithramma P. Dinesh-Kumar, and Yongliang Zhang
- Subjects
Multidisciplinary - Abstract
Nucleotide-binding leucine-rich repeat (NLR) receptor-mediated immunity includes rapid production of reactive oxygen species (ROS) and transcriptional reprogramming, which is controlled by transcription factors (TFs). Although some TFs have been reported to participate in NLR-mediated immune response, most TFs are transcriptional activators, and whether and how transcriptional repressors regulate NLR-mediated plant defenses remains largely unknown. Here, we show that the Alfin-like 7 (AL7) interacts with N NLR and functions as a transcriptional repressor. Knockdown and knockout of AL7 compromise N NLR -mediated resistance against tobacco mosaic virus, whereas AL7 overexpression enhances defense, indicating a positive regulatory role for AL7 in immunity. AL7 binds to the promoters of ROS scavenging genes to inhibit their transcription during immune responses. Mitogen-activated protein kinases (MAPKs), salicylic acid-induced protein kinase (SIPK), and wound-induced protein kinase (WIPK) directly interact with and phosphorylate AL7, which impairs the AL7-N interaction and enhances its DNA binding activity, which promotes ROS accumulation and enables immune activation. In addition to N, AL7 is also required for the function of other Toll interleukin 1 receptor/nucleotide-binding/leucine-rich repeats (TNLs) including Roq1 and RRS1-R/RPS4. Our findings reveal a hitherto unknown MAPK-AL7 module that negatively regulates ROS scavenging genes to promote NLR-mediated immunity.
- Published
- 2023
3. SAMDC3 enhances resistance to Barley stripe mosaic virus by promoting the ubiquitination and proteasomal degradation of viral γb protein
- Author
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Zhaolei Li, Xinxin Yang, Wenli Li, Zhiyan Wen, Jiangning Duan, Zhihao Jiang, Dingliang Zhang, Xialin Xie, Xueting Wang, Fangfang Li, Dawei Li, and Yongliang Zhang
- Subjects
Adenosylmethionine Decarboxylase ,Viral Proteins ,Physiology ,Ubiquitination ,Hordeum ,Plant Science ,Plant Viruses - Abstract
Posttranslational modifications (PTMs) play important roles in virus-host interplay. We previously demonstrated that Barley stripe mosaic virus (BSMV) γb protein is phosphorylated by different host kinases to support or impede viral infection. However, whether and how other types of PTMs participate in BSMV infection remains to be explored. Here, we report that S-adenosylmethionine decarboxylase 3 (SAMDC3) from Nicotiana benthamiana or wheat (Triticum aestivum) interacts with γb. BSMV infection induced SAMDC3 expression. Overexpression of SAMDC3 led to the destabilization of γb and reduction in viral infectivity, whereas knocking out NbSAMDC3 increased susceptibility to BSMV. NbSAMDC3 positively regulated the 26S proteasome-mediated degradation of γb via its PEST domain. Further mechanistic studies revealed that γb can be ubiquitinated in planta and that NbSAMDC3 promotes the proteasomal degradation of γb by increasing γb ubiquitination. We also found evidence that ubiquitination occurs at nonlysine residues (Ser-133 and Cys-144) within γb. Together, our results provide a function for SAMDC3 in defence against BSMV infection through targeting of γb abundance, which contributes to our understanding of how a plant host deploys the ubiquitin-proteasome system to mount defences against viral infections.
- Published
- 2021
4. Highly efficient heritable genome editing in wheat using an RNA virus and bypassing tissue culture
- Author
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Caixia Gao, Yanpeng Wang, Tingdong Li, Boshu Li, Yu Sun, Hu Jiacheng, Jinxing Liu, Yongliang Zhang, Dawei Li, Dingliang Zhang, and Wenli Li
- Subjects
Genetics ,Gene Editing ,Barley stripe mosaic virus ,biology ,Mutant ,Inheritance Patterns ,food and beverages ,Mutagenesis (molecular biology technique) ,RNA virus ,Plant Science ,biology.organism_classification ,Plants, Genetically Modified ,Virus ,Plant Viruses ,Genome editing ,CRISPR-Associated Protein 9 ,Common wheat ,Molecular Biology ,Genome, Plant ,Triticum ,Subgenomic mRNA ,RNA, Guide, Kinetoplastida - Abstract
Genome editing provides novel strategies for improving plant traits but mostly relies on conventional plant genetic transformation and regeneration procedures, which can be inefficient. In this study, we have engineered a Barley stripe mosaic virus-based sgRNA delivery vector (BSMV-sg) that is effective in performing heritable genome editing in Cas9-transgenic wheat plants. Mutated progenies were present in the next generation at frequencies ranging from 12.9% to 100% in three different wheat varieties, and 53.8%-100% of mutants were virus free. We also achieved multiplex mutagenesis in progeny using a pool of BSMV-sg vectors harboring different sgRNAs. Furthermore, we devised a virus-induced transgene-free editing procedure to generate Cas9-free wheat mutants by crossing BSMV-infected Cas9-transgenic wheat pollen with wild-type wheat. Our study provides a robust, convenient, and tissue culture-free approach for genome editing in wheat through virus infection.
- Published
- 2021
5. Proximity labeling: an emerging tool for probing in planta molecular interactions
- Author
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Zhen Li, Yongliang Zhang, Savithramma P. Dinesh-Kumar, Xinxin Yang, Dingliang Zhang, Zhiyan Wen, Dawei Li, and Ugrappa Nagalakshmi
- Subjects
Cellular activity ,Quantitative proteomics ,membrane contact sites ,plant ,Plant Science ,Computational biology ,Review Article ,Biology ,Biochemistry ,protein interactions ,Protein–protein interaction ,Protein Interaction Maps ,Plant system ,Molecular Biology ,Organism ,Plant Proteins ,Molecular interactions ,Staining and Labeling ,Botany ,Cell Biology ,Plant biology ,organelles ,Identification (biology) ,biotin ligase ,Biotechnology ,proximity labeling - Abstract
Protein–protein interaction (PPI) networks are key to nearly all aspects of cellular activity. Therefore, the identification of PPIs is important for understanding a specific biological process in an organism. Compared with conventional methods for probing PPIs, the recently described proximity labeling (PL) approach combined with mass spectrometry (MS)-based quantitative proteomics has emerged as a powerful approach for characterizing PPIs. However, the application of PL in planta remains in its infancy. Here, we summarize recent progress in PL and its potential utilization in plant biology. We specifically summarize advances in PL, including the development and comparison of different PL enzymes and the application of PL for deciphering various molecular interactions in different organisms with an emphasis on plant systems., Proximity labeling (PL) has emerged as a powerful approach for the characterization of protein–protein interactions. This review summarizes advances in PL, including the development and comparison of different PL enzymes and the application of PL for dissecting molecular interactions in different organisms with an emphasis on plant systems.
- Published
- 2020
6. Genome-wide transcriptional profiling reveals molecular signatures of secondary xylem differentiation in Populus tomentosa
- Author
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Xinguo Li, Bailian Li, Dingliang Zhang, and Xu-Hua Yang
- Subjects
Transcription, Genetic ,Biology ,Real-Time Polymerase Chain Reaction ,Lignin ,Transcriptome ,Cell wall ,Cell Wall ,Gene Expression Regulation, Plant ,Xylem ,Gene expression ,Botany ,Genetics ,Vascular cambium ,Molecular Biology ,Oligonucleotide Array Sequence Analysis ,Regulation of gene expression ,Microarray analysis techniques ,Gene Expression Profiling ,Cell Cycle ,fungi ,Reproducibility of Results ,food and beverages ,Cell Differentiation ,Molecular Sequence Annotation ,General Medicine ,Cell biology ,Gene expression profiling ,Gene Ontology ,Populus ,Genome, Plant ,Transcription Factors - Abstract
Wood formation occurs via cell division, primary cell wall and secondary wall formation, and programmed cell death in the vascular cambium. Transcriptional profiling of secondary xylem differentiation is essential for understanding the molecular mechanisms underlying wood formation. Differential gene expression in secondary xylem differentiation of Populus has been previously investigated using cDNA microarray analysis. However, little is known about the molecular mechanisms from a genome-wide perspective. In this study, the Affymetrix poplar genome chips containing 61,413 probes were used to investigate the changes in the transcriptome during secondary xylem differentiation in Chinese white poplar (Populus tomentosa). Two xylem tissues (newly formed and lignified) were sampled for genome-wide transcriptional profiling. In total, 6843 genes (~11%) were identified with differential expression in the two xylem tissues. Many genes involved in cell division, primary wall modification, and cellulose synthesis were preferentially expressed in the newly formed xylem. In contrast, many genes, including 4-coumarate:cinnamate-4-hydroxylase (C4H), 4-coumarate:CoA ligase (4CL), cinnamyl alcohol dehydrogenase (CAD), and caffeoyl CoA 3-O-methyltransferase (CCoAOMT), associated with lignin biosynthesis were more transcribed in the lignified xylem. The two xylem tissues also showed differential expression of genes related to various hormones; thus, the secondary xylem differentiation could be regulated by hormone signaling. Furthermore, many transcription factor genes were preferentially expressed in the lignified xylem, suggesting that wood lignification involves extensive transcription regulation. The genome-wide transcriptional profiling of secondary xylem differentiation could provide additional insights into the molecular basis of wood formation in poplar species.
- Published
- 2014
7. A 19F nuclear magnetic resonance study of the conjugate Brönsted-Lewis superacid HSO3F-SbF5. Part 1
- Author
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Dingliang Zhang, Markus Heubes, Gerhard Hägele, and Friedhelm Aubke
- Subjects
Organic Chemistry ,General Chemistry ,Catalysis - Abstract
The Brönsted-Lewis superacid HSO3F-SbF5 or "magic acid" is re-investigated by modern 19F NMR methods over a wide concentration range. The system is found to be considerably more complex than had been assumed previously. A total of 13 different anions are identified of which only five have previously been identified in magic acid. With increasing SbF5 contents the concentration of monomeric anions like [SbF6]-, [SbF5(SO3F)]-, cis- and trans-[SbF4(SO3F)2]-, and mer-[SbF3(SO3F)3]- gradually decreases. Except for [Sb2F11]-, which is present in very small concentrations only, the formation of oligomers involves exclusively μ-fluorosulfato bridges. In addition to donor (SO3F)- and acceptor (SbF5) complex formation to give [SbF5(SO3F)]- and possibly ligand redistribution, the solvolysis of SbF5 or SbF4(SO3F) in HSO3F appears to be the principal formation reaction for polyfluorosulfatofluoroantimonate(V) anions. In glass (NMR tubes) the solvolysis product HF is converted to the oxonium ion [H3O]+, which has previously been identified by 1H NMR and structurally characterized as [H3O][Sb2F11] by us.Key words: magic acid, conjugate superacid, fluorosulfuric acid, 19F NMR spectra.
- Published
- 1999
8. ChemInform Abstract: A 19F Nuclear Magnetic Resonance Study of the Conjugate Broensted-Lewis Superacid HSO3F-SbF5. Part 1
- Author
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Dingliang Zhang, Friedhelm Aubke, Markus Heubes, and Gerhard Haegele
- Subjects
chemistry.chemical_compound ,Nuclear magnetic resonance ,Chemistry ,General Medicine ,Superacid ,Conjugate - Published
- 2010
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