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Proximity labeling: an emerging tool for probing in planta molecular interactions
- Source :
- Plant Communications
- Publication Year :
- 2020
- Publisher :
- Elsevier, 2020.
-
Abstract
- Protein–protein interaction (PPI) networks are key to nearly all aspects of cellular activity. Therefore, the identification of PPIs is important for understanding a specific biological process in an organism. Compared with conventional methods for probing PPIs, the recently described proximity labeling (PL) approach combined with mass spectrometry (MS)-based quantitative proteomics has emerged as a powerful approach for characterizing PPIs. However, the application of PL in planta remains in its infancy. Here, we summarize recent progress in PL and its potential utilization in plant biology. We specifically summarize advances in PL, including the development and comparison of different PL enzymes and the application of PL for deciphering various molecular interactions in different organisms with an emphasis on plant systems.<br />Proximity labeling (PL) has emerged as a powerful approach for the characterization of protein–protein interactions. This review summarizes advances in PL, including the development and comparison of different PL enzymes and the application of PL for dissecting molecular interactions in different organisms with an emphasis on plant systems.
- Subjects :
- Cellular activity
Quantitative proteomics
membrane contact sites
plant
Plant Science
Computational biology
Review Article
Biology
Biochemistry
protein interactions
Protein–protein interaction
Protein Interaction Maps
Plant system
Molecular Biology
Organism
Plant Proteins
Molecular interactions
Staining and Labeling
Botany
Cell Biology
Plant biology
organelles
Identification (biology)
biotin ligase
Biotechnology
proximity labeling
Subjects
Details
- Language :
- English
- ISSN :
- 25903462
- Volume :
- 2
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Plant Communications
- Accession number :
- edsair.doi.dedup.....1c8249f4eeef93f06922e1e4803ea54f