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4. QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy

Author

Nelson, G., Boehm, Ulrike, Bagley, S., Bajcsy, P., Bischof, J., Brown, Claire M., Dauphin, A., Dobbie, I.M., Eriksson, J.E., Faklaris, O., Fernandez-Rodriguez, J., Ferrand, A., Gelman, L., Gheisari, A., Hartmann, H., Kukat, C., Laude, A., Mitkovski, M., Munck, S., North, A.J., Rasse, T.M., Resch-Genger, U., Schuetz, L.C., Seitz, A., Strambio-De-Castillia, C., Swedlow, J.R., Alexopoulos, I., Aumayr, K., Avilov, S., Bakker, G.J., Bammann, R.R., Bassi, A., Beckert, H., Beer, S., Belyaev, Y., Bierwagen, J., Birngruber, K.A., Bosch, M., Breitlow, J., Cameron, L.A., Chalfoun, J., Chambers, J.J., Chen, C.L., Conde-Sousa, E., Corbett, A.D., Cordelieres, F.P., Nery, E.D., Dietzel, R., Eismann, F., Fazeli, E., Felscher, A., Fried, H., Gaudreault, N., Goh, W.I., Guilbert, T., Hadleigh, R., Hemmerich, P., Holst, G.A., Itano, M.S., Jaffe, C.B., Jambor, H.K., Jarvis, S.C., Keppler, A., Kirchenbuechler, D., Kirchner, M., Kobayashi, N., Krens, G., Kunis, S., Lacoste, J., Marcello, M., Martins, G.G., Metcalf, D.J., Mitchell, C.A., Moore, J., Mueller, T., Nelson, M.S., Ogg, S., Onami, S., Palmer, A.L., Paul-Gilloteaux, P., Pimentel, J.A., Plantard, L., Podder, S., Rexhepaj, E., Royon, A., Saari, M.A., Schapman, D., Schoonderwoert, V., Schroth-Diez, B., Schwartz, S., Shaw, M., Spitaler, M., Stoeckl, M.T., Sudar, D., Teillon, J., Terjung, S., Thuenauer, R., Wilms, C.D., Wright, G.D., Nitschke, R., Nelson, G., Boehm, Ulrike, Bagley, S., Bajcsy, P., Bischof, J., Brown, Claire M., Dauphin, A., Dobbie, I.M., Eriksson, J.E., Faklaris, O., Fernandez-Rodriguez, J., Ferrand, A., Gelman, L., Gheisari, A., Hartmann, H., Kukat, C., Laude, A., Mitkovski, M., Munck, S., North, A.J., Rasse, T.M., Resch-Genger, U., Schuetz, L.C., Seitz, A., Strambio-De-Castillia, C., Swedlow, J.R., Alexopoulos, I., Aumayr, K., Avilov, S., Bakker, G.J., Bammann, R.R., Bassi, A., Beckert, H., Beer, S., Belyaev, Y., Bierwagen, J., Birngruber, K.A., Bosch, M., Breitlow, J., Cameron, L.A., Chalfoun, J., Chambers, J.J., Chen, C.L., Conde-Sousa, E., Corbett, A.D., Cordelieres, F.P., Nery, E.D., Dietzel, R., Eismann, F., Fazeli, E., Felscher, A., Fried, H., Gaudreault, N., Goh, W.I., Guilbert, T., Hadleigh, R., Hemmerich, P., Holst, G.A., Itano, M.S., Jaffe, C.B., Jambor, H.K., Jarvis, S.C., Keppler, A., Kirchenbuechler, D., Kirchner, M., Kobayashi, N., Krens, G., Kunis, S., Lacoste, J., Marcello, M., Martins, G.G., Metcalf, D.J., Mitchell, C.A., Moore, J., Mueller, T., Nelson, M.S., Ogg, S., Onami, S., Palmer, A.L., Paul-Gilloteaux, P., Pimentel, J.A., Plantard, L., Podder, S., Rexhepaj, E., Royon, A., Saari, M.A., Schapman, D., Schoonderwoert, V., Schroth-Diez, B., Schwartz, S., Shaw, M., Spitaler, M., Stoeckl, M.T., Sudar, D., Teillon, J., Terjung, S., Thuenauer, R., Wilms, C.D., Wright, G.D., and Nitschke, R.

Abstract

Item does not contain fulltext, A modern day light microscope has evolved from a tool devoted to making primarily empirical observations to what is now a sophisticated , quantitative device that is an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use in capturing and quantifying scientific phenomena, neither a thorough understanding of the principles underlying quantitative imaging techniques nor appropriate knowledge of how to calibrate, operate and maintain microscopes can be taken for granted. This is clearly demonstrated by the well-documented and widespread difficulties that are routinely encountered in evaluating acquired data and reproducing scientific experiments. Indeed, studies have shown that more than 70% of researchers have tried and failed to repeat another scientist's experiments, while more than half have even failed to reproduce their own experiments. One factor behind the reproducibility crisis of experiments published in scientific journals is the frequent underreporting of imaging methods caused by a lack of awareness and/or a lack of knowledge of the applied technique. Whereas quality control procedures for some methods used in biomedical research, such as genomics (e.g. DNA sequencing, RNA-seq) or cytometry, have been introduced (e.g. ENCODE), this issue has not been tackled for optical microscopy instrumentation and images. Although many calibration standards and protocols have been published, there is a lack of awareness and agreement on common standards and guidelines for quality assessment and reproducibility. In April 2020, the QUality Assessment and REProducibility for instruments and images in Light Microscopy (QUAREP-LiMi) initiative was formed. This initiative comprises imaging scientists from academia and industry who share a common interest in achieving a better understanding of the performance and limitations of microscopes and improved quality con

Published
2021

5. Mechanography performance tests and their association with sarcopenia, falls and impairment in the activities of daily living – a pilot cross-sectional study in 293 older adults

Author

Dietzel, R., Felsenberg, D., and Armbrecht, G.

Subjects
Aged, 80 and over, Male, Sarcopenia, Pilot Projects, Middle Aged, Biomechanical Phenomena, Cross-Sectional Studies, Activities of Daily Living, Mechanography, Humans, Original Article, Falls, Muscle Power, Accidental Falls, Female, Muscle Strength, Muscle, Skeletal, human activities, Aged
Abstract

Objectives: Muscle mass and muscle power considerably decline with aging. The aim of the present study was to determine the association between muscular function using mechanography and sarcopenia, falls and impairment in the activities of daily living (ADL) in a sample of 293 community-dwelling women and men aged 60-85 years in Berlin, Germany. Methods: Muscle function was determined by muscle power per body mass in vertical countermovement jumps (2LJPrel) and the chair rising test (CRTPrel) on a force plate. Sarcopenia status was assessed by estimating appendicular muscle mass with dual-X-ray absorptiometry. Self-reported ADL impairment and falls in the last 12 months were determined. Results: ADL impairment was significantly correlated with all performance tests but not with muscle mass. The 2LJPrel (OR 0.88, 95%-CI 0.79-0.98), the Esslinger Fitness Index (EFI) (OR 0.97, 95%-CI 0.94-1.00) and the maximal velocity of the CRT (OR 0.70, 95%-CI 0.53-0.93) remained significant correlates for sarcopenia independent of age in men but not in women. The EFI could differentiate female individuals who had past fall events (OR 0.96, 95%-CI 0.93-0.98). Conclusion: The results of the present study highlight the importance of assessing muscle power in older individuals as a relevant correlate for functional decline.

Published
2015

8. Wein

Author

Fabre, J. H., Brémond, E., Semichon, L., Manceau, E., Roos, L., Marcelet, H., Bespaloff, Sch, Mummendy, R., Malvezin, Ph., Essner, J. Ch., Moreau, L., Vinet, E., Cohn, M. S. R., Richarz, J., Herzberg, Reinbeck, M., Werder, J., Eiduschka, A., Schrempf, A., Fiesselmann, G., Reif, G., Bleyer, B., Diemair, W., Lix, G., Jahn, R., Heide, C. v. d., Schindler, J., Koazk, I., Klostermann, M., Fachmann, W., Litterscheid, F. M., Röttgen, Th., Heiduschka, A., Pyriki, C., Kloss, J., Müller, K., Vogt, E., Raesch, O., Kober, H., Petri, W., Wirthle, F., Benz, G., Rüdiger, M., Alfa, J., Schätzlein, Ch., Wüstenfeld, H., Luckow, E., Walter, E., Seifert, W., Schulte, W., Gardiner, Brauer, K., Seyda, A., Plahl, W., Ofner, R., Wilhelm, C., alberer, O. E., Widmer, A., Dietzel, R., Taufel, K., Zách, C., Miermeister, A., Battay, F., Großfeld, J., Ferré, L., Crisci, P., Astruc, H., Aubour, P., Semichen, L., Flanzy, M., Martin, M., Jeanprêtre, J., Volmar, Y., Clavera, J. M., Benvegnin, L., Capt, E., Hugues, E., Baragiola, W. J., Godet, Ch., Michel, A., von Fellenberg, Th., Fischer, R., Stauder, Fr., Fonces-Diacon, Laforce, Bertin, Ch., Ventre, J., Bouffard, E., Dubaquié, J., Debordes, G., Lamazou-Betbeder, M., Ribéreau-Gayon, J., and Marcille, R.

Published
1932
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10. Bücherbesprechungen

Author

O., Wo., Liesegang, R. E., Thiessen, Naeser, Hüttig, G. F., Scheibitz, F. -K., Dietzel, R., Beythien, A., Jäger, and Michael, H.

Published
1943
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13. Bücherbesprechungen

Author

Erbring, H., Menzel, Heinrich, Lange, E., Fehér, F., Sirk, H., Liesegang, R. E., Philippoff, Kauffmann, H., and Dietzel, R.

Published
1940
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14. Arsen

Author

Kahane, E., Liem, H. T., Niederer, Christensen, E. V., Green, L. W., Schoetzow, R. E., Dietzel, R., Sieger, M., Rupp, E., Hamann, G., Cocking, T. T., Escolar, Carmen G., Taubmann, G., van't Spijker, J. N., van Giffen, H. J., Wagenaar, M., Keimatsu, S., Wada, K., Szlatinay, L., Schulek, E., Kühl, H., and Czyzewsky, B.

Published
1937
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21. Literatur

Author

Abegg, R., Auerbach, Fr., Jander, W., Kurtenacker, A., Siemens, A., Smith, A., D'Ans, J., Haber, F., Smith-Haber, Feitknecht, W., Kohlschütter, H. W., Kohlschütter, V., Henrich, F., Ober, E., Merck, E., Emich, F., Feigl, F., Beckurts, H., Berg, R., Dietzel, R., Lüning, O., and Teitelbaum, M.

Published
1931
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26. Leitlinie Physiotherapie und Bewegungstherapie bei Osteoporose

Author

Dietzel, R., primary, Begerow, B., additional, Bollert, G., additional, Kladny, B., additional, Kleinöder, H., additional, Kwiet, A., additional, Pientka, L., additional, Preisinger, E., additional, Runge, M., additional, Swanenburg, J., additional, Wieland, E., additional, and Felsenberg, D., additional

Published
2009
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30. Radiometric Analysis of Laser Modulated IR Properties of Semiconductors.

Author

Dietzel, R., Gibkes, J., Chotikaprakham, S., Bein, B.K., and Pelzl, J.

Subjects
SEMICONDUCTORS, NUCLEAR activation analysis, INFRARED radiation, OPTICAL properties
Abstract

A photothermal technique for the characterization of semiconductor materials is presented, in combination with the theoretical description of the signal generation process of the effects of the charge carrier density on the IR optical properties. It relies on the excitation of charge carrier density waves by modulated laser irradiation in the visible spectrum, leading to periodical variations of the IR optical properties. The detection is based on sensing the ir transmission of the semiconductor sample. The modulated laser irradiation in the visible simultaneously leads to small temperature variations and additional signal contributions due to the modulation of the internal IR radiation, which can be minimized and eliminated by appropriate focussing conditions. A principal understanding of the signal generation mechanism has been achieved by timedependent measurements with a gradually increasing intensity of the external IR radiation source, while frequency-dependent measurements of the modulated IR transmission signal provide quantitative information on the semiconductor properties. [ABSTRACT FROM AUTHOR]

Published
2003

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