Search

Your search keyword '"Diane Bigot"' showing total 17 results
Start Over Author "Diane Bigot"
17 results on '"Diane Bigot"'

1. Epidemiology of a major honey bee pathogen, deformed wing virus: potential worldwide replacement of genotype A by genotype B

Author

Robert J. Paxton, Marc O. Schäfer, Francesco Nazzi, Virginia Zanni, Desiderato Annoscia, Fabio Marroni, Diane Bigot, Eoin R. Laws-Quinn, Delphine Panziera, Christina Jenkins, and Hassan Shafiey

Subjects
Apis mellifera, RNA, DWV, Iflaviridae, Evolution, Recombination, Zoology, QL1-991
Abstract

The western honey bee (Apis mellifera) is of major economic and ecological importance, with elevated rates of colony losses in temperate regions over the last two decades thought to be largely caused by the exotic ectoparasitic mite Varroa destructor and deformed wing virus (DWV), which the mite transmits. DWV currently exists as two main genotypes: the formerly widespread DWV-A and the more recently described and rapidly expanding DWV-B. It is an excellent system to understand viral evolution and the replacement of one viral variant by another. Here we synthesise published results on the distribution and prevalence of DWV-A and -B over the period 2008–2021 and present novel data for Germany, Italy and the UK to suggest that (i) DWV-B has rapidly expanded worldwide since its first description in 2004 and (ii) that it is potentially replacing DWV-A. Both genotypes are also found in wild bee species. Based on a simple mathematical model, we suggest that interference between viral genotypes when co-infecting the same host is key to understanding their epidemiology. We finally discuss the consequences of genotype replacement for beekeeping and for wild pollinator species.

Published
2022
Full Text
View/download PDF

2. Chromosomal scale assembly of parasitic wasp genome reveals symbiotic virus colonization

Author

Jérémy Gauthier, Hélène Boulain, Joke J. F. A. van Vugt, Lyam Baudry, Emma Persyn, Jean-Marc Aury, Benjamin Noel, Anthony Bretaudeau, Fabrice Legeai, Sven Warris, Mohamed A. Chebbi, Géraldine Dubreuil, Bernard Duvic, Natacha Kremer, Philippe Gayral, Karine Musset, Thibaut Josse, Diane Bigot, Christophe Bressac, Sébastien Moreau, Georges Periquet, Myriam Harry, Nicolas Montagné, Isabelle Boulogne, Mahnaz Sabeti-Azad, Martine Maïbèche, Thomas Chertemps, Frédérique Hilliou, David Siaussat, Joëlle Amselem, Isabelle Luyten, Claire Capdevielle-Dulac, Karine Labadie, Bruna Laís Merlin, Valérie Barbe, Jetske G. de Boer, Martial Marbouty, Fernando Luis Cônsoli, Stéphane Dupas, Aurélie Hua-Van, Gaelle Le Goff, Annie Bézier, Emmanuelle Jacquin-Joly, James B. Whitfield, Louise E. M. Vet, Hans M. Smid, Laure Kaiser, Romain Koszul, Elisabeth Huguet, Elisabeth A. Herniou, and Jean-Michel Drezen

Subjects
Biology (General), QH301-705.5
Abstract

Jeremy Gauthier et al. present the chromosome scale assembly of the genome of the parasitic wasp C. congregata and show that bracovirus genes have colonized all ten chromosomes. Comparison with genome scaffolds of another wasp reveals a striking stability of these regions over ~53 million years, suggesting strong evolutionary constraints.

Published
2021
Full Text
View/download PDF

3. Author Correction: Chromosomal scale assembly of parasitic wasp genome reveals symbiotic virus colonization

Author

Jérémy Gauthier, Hélène Boulain, Joke J. F. A. van Vugt, Lyam Baudry, Emma Persyn, Jean-Marc Aury, Benjamin Noel, Anthony Bretaudeau, Fabrice Legeai, Sven Warris, Mohamed A. Chebbi, Géraldine Dubreuil, Bernard Duvic, Natacha Kremer, Philippe Gayral, Karine Musset, Thibaut Josse, Diane Bigot, Christophe Bressac, Sébastien Moreau, Georges Periquet, Myriam Harry, Nicolas Montagné, Isabelle Boulogne, Mahnaz Sabeti-Azad, Martine Maïbèche, Thomas Chertemps, Frédérique Hilliou, David Siaussat, Joëlle Amselem, Isabelle Luyten, Claire Capdevielle-Dulac, Karine Labadie, Bruna Laís Merlin, Valérie Barbe, Jetske G. de Boer, Martial Marbouty, Fernando Luis Cônsoli, Stéphane Dupas, Aurélie Hua-Van, Gaelle Le Goff, Annie Bézier, Emmanuelle Jacquin-Joly, James B. Whitfield, Louise E. M. Vet, Hans M. Smid, Laure Kaiser, Romain Koszul, Elisabeth Huguet, Elisabeth A. Herniou, and Jean-Michel Drezen

Subjects
Biology (General), QH301-705.5
Published
2021
Full Text
View/download PDF

4. Viruses in the Invasive Hornet Vespa velutina

Author

Anne Dalmon, Philippe Gayral, Damien Decante, Christophe Klopp, Diane Bigot, Maxime Thomasson, Elisabeth A Herniou, Cédric Alaux, and Yves Le Conte

Subjects
vespidae, invasive species, honey bee viruses, new viruses, dwv, abpv, bqcv, kbv, alpv, beemlv, Microbiology, QR1-502
Abstract

The Asian yellow-legged hornet Vespa velutina nigrithorax, a major predator of honeybees, is spreading in Europe in part due to a lack of efficient control methods. In this study, as a first step to identify biological control agents, we characterized viral RNA sequences present in asymptomatic or symptomatic hornets. Among 19 detected viruses, the honey bee virus Deformed wing virus-B was predominant in all the samples, particularly in muscles from the symptomatic hornet, suggesting a putative cause of the deformed wing symptom. Interestingly, two new viruses closely related to Acyrthosiphon pisum virus and Himetobi P virus and viruses typically associated with honey bees, Acute bee paralysis virus and Black queen cell virus, were detected in the brain and muscles, and may correspond to the circulation and possible replication forms of these viruses in the hornet. Aphid lethal paralysis virus, Bee Macula-like virus, and Moku virus, which are known to infect honey bees, were also identified in the gut virus metagenome of hornets. Therefore, our study underlined the urgent need to study the host range of these newly discovered viruses in hornets to determine whether they represent a new threat for honey bees or a hope for the biocontrol of V. velutina.

Published
2019
Full Text
View/download PDF

5. The Two Prevalent Genotypes of an Emerging Infectious Disease, Deformed Wing Virus, Cause Equally Low Pupal Mortality and Equally High Wing Deformities in Host Honey Bees

Author

Anja Tehel, Quynh Vu, Diane Bigot, Andreas Gogol-Döring, Peter Koch, Christina Jenkins, Vincent Doublet, Panagiotis Theodorou, and Robert Paxton

Subjects
pathology, virulence, positive single-strand RNA virus, DWV, genotype A, genotype B, Apis rhadbovirus-1, Apis mellifera, Microbiology, QR1-502
Abstract

Deformed wing virus (DWV) is an emerging infectious disease of the honey bee (Apis mellifera) that is considered a major cause of elevated losses of honey bee colonies. DWV comprises two widespread genotypes: the originally described genotype A, and genotype B. In adult honey bees, DWV-B has been shown to be more virulent than DWV-A. However, their comparative effects on earlier host developmental stages are unknown. Here, we experimentally inoculated honey bee pupae and tested for the relative impact of DWV-A versus DWV-B on mortality and wing deformities in eclosing adults. DWV-A and DWV-B caused similar, and only slightly elevated, pupal mortality (mean 18% greater mortality than control). Both genotypes caused similarly high wing deformities in eclosing adults (mean 60% greater wing deformities than control). Viral titer was high in all of the experimentally inoculated eclosing adults, and was independent of wing deformities, suggesting that the phenotype ‘deformed wings’ is not directly related to viral titer or viral genotype. These viral traits favor the emergence of both genotypes of DWV by not limiting the reproduction of its vector, the ectoparasitic Varroa destructor mite, in infected pupae, and thereby facilitating the spread of DWV in honey bees infested by the mite.

Published
2019
Full Text
View/download PDF

6. Comparative Analysis of Transcriptomes from Secondary Reproductives of Three Reticulitermes Termite Species.

Author

Franck Dedeine, Lucy A Weinert, Diane Bigot, Thibaut Josse, Marion Ballenghien, Vincent Cahais, Nicolas Galtier, and Philippe Gayral

Subjects
Medicine, Science
Abstract

Termites are eusocial insects related to cockroaches that feed on lignocellulose. These insects are key species in ecosystems since they recycle a large amount of nutrients but also are pests, exerting major economic impacts. Knowledge on the molecular pathways underlying reproduction, caste differentiation or lignocellulose digestion would largely benefit from additional transcriptomic data. This study focused on transcriptomes of secondary reproductive females (nymphoid neotenics). Thirteen transcriptomes were used: 10 of Reticulitermes flavipes and R. grassei sequenced from a previous study, and two transcriptomes of R. lucifugus sequenced for the present study. After transcriptome assembly and read mapping, we examined interspecific variations of genes expressed by termites or gut microorganisms. A total of 18,323 orthologous gene clusters were detected. Functional annotation and taxonomic assignment were performed on a total of 41,287 predicted contigs in the three termite species. Between the termite species studied, functional categories of genes were comparable. Gene ontology (GO) terms analysis allowed the discovery of 9 cellulases and a total of 79 contigs potentially involved in 11 enzymatic activities used in wood metabolism. Altogether, results of this study illustrate the strong potential for the use of comparative interspecific transcriptomes, representing a complete resource for future studies including differentially expressed genes between castes or SNP analysis for population genetics.

Published
2015
Full Text
View/download PDF

7. Author Correction: Chromosomal scale assembly of parasitic wasp genome reveals symbiotic virus colonization

Author

Martine Maïbèche, Hélène Boulain, Christophe Bressac, Laure Kaiser, Emma Persyn, Valérie Barbe, Anthony Bretaudeau, Martial Marbouty, Bernard Duvic, Georges Periquet, Annie Bézier, Lyam Baudry, Jean-Marc Aury, Mahnaz Sabeti-Azad, Géraldine Dubreuil, Karine Labadie, Fernando Luis Cônsoli, Benjamin Noel, Frédérique Hilliou, Thomas Chertemps, Elisabeth A. Herniou, Mohamed Amine Chebbi, Fabrice Legeai, Jérémy Gauthier, Jetske G. de Boer, Nicolas Montagné, Joelle Amselem, Jean-Michel Drezen, Thibaut Josse, Sébastien J. M. Moreau, Sven Warris, Louise E. M. Vet, Isabelle Boulogne, Myriam Harry, Diane Bigot, Elisabeth Huguet, Karine Musset, Natacha Kremer, Claire Capdevielle-Dulac, Joke J.F.A. van Vugt, James B. Whitfield, Aurélie Hua-Van, Hans M. Smid, Gaëlle Le Goff, Romain Koszul, Bruna Laís Merlin, Stéphane Dupas, Isabelle Luyten, Philippe Gayral, Emmanuelle Jacquin-Joly, and David Siaussat

Subjects
Scale (ratio), QH301-705.5, Genome, Insect, Wasps, Medicine (miscellaneous), Computational biology, Biology, Receptors, Odorant, Synteny, Genome, Evolutionary genetics, General Biochemistry, Genetics and Molecular Biology, Virus, 03 medical and health sciences, 0302 clinical medicine, Animals, Nudiviridae, Colonization, Biology (General), Author Correction, Symbiosis, Conserved Sequence, 030304 developmental biology, 0303 health sciences, Base Sequence, Biological Evolution, Genome evolution, Chromosomes, Insect, Smell, Polydnaviridae, General Agricultural and Biological Sciences, Entomology, 030217 neurology & neurosurgery
Abstract

Endogenous viruses form an important proportion of eukaryote genomes and a source of novel functions. How large DNA viruses integrated into a genome evolve when they confer a benefit to their host, however, remains unknown. Bracoviruses are essential for the parasitism success of parasitoid wasps, into whose genomes they integrated ~103 million years ago. Here we show, from the assembly of a parasitoid wasp genome at a chromosomal scale, that bracovirus genes colonized all ten chromosomes of Cotesia congregata. Most form clusters of genes involved in particle production or parasitism success. Genomic comparison with another wasp, Microplitis demolitor, revealed that these clusters were already established ~53 mya and thus belong to remarkably stable genomic structures, the architectures of which are evolutionary constrained. Transcriptomic analyses highlight temporal synchronization of viral gene expression without resulting in immune gene induction, suggesting that no conflicts remain between ancient symbiotic partners when benefits to them converge.

Published
2021
Full Text
View/download PDF

8. Chromosomal scale assembly of parasitic wasp genome reveals symbiotic virus colonization

Author

Myriam Harry, Bernard Duvic, Anthony Bretaudeau, Hélène Boulain, Jetske G. de Boer, Jean-Marc Aury, Claire Capdevielle-Dulac, Annie Bézier, Diane Bigot, Natacha Kremer, Laure Kaiser, Lyam Baudry, Joke J.F.A. van Vugt, Jean-Michel Drezen, Georges Periquet, Stéphane Dupas, Romain Koszul, Aurélie Hua-Van, Jérémy Gauthier, Fabrice Legeai, Emma Persyn, Mahnaz Sabeti-Azad, Fernando Luis Cônsoli, Valérie Barbe, Joelle Amselem, Karine Labadie, Benjamin Noel, Frédérique Hilliou, Martine Maïbèche, Géraldine Dubreuil, Isabelle Luyten, Karine Musset, Martial Marbouty, Christophe Bressac, Isabelle Boulogne, Louise E. M. Vet, Thibaut Josse, Philippe Gayral, Hans M. Smid, Emmanuelle Jacquin-Joly, David Siaussat, James B. Whitfield, Elisabeth A. Herniou, Mohamed Amine Chebbi, Thomas Chertemps, Nicolas Montagné, Sébastien J. M. Moreau, Elisabeth Huguet, Bruna Laís Merlin, Gaëlle Le Goff, Sven Warris, Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours-Centre National de la Recherche Scientifique (CNRS), Natural History Museum [Geneva], Swiss Federal Insitute of Aquatic Science and Technology [Dübendorf] (EAWAG), Netherlands Institute of Ecology - NIOO-KNAW (NETHERLANDS), Régulation spatiale des Génomes - Spatial Regulation of Genomes, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Collège doctoral [Sorbonne universités], Sorbonne Université (SU), Institut d'écologie et des sciences de l'environnement de Paris (iEES Paris ), Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Génomique métabolique (UMR 8030), Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université d'Évry-Val-d'Essonne (UEVE), Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Université de Bretagne Sud (UBS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National de Recherche en Informatique et en Automatique (Inria)-École normale supérieure - Rennes (ENS Rennes)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-CentraleSupélec-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Scalable, Optimized and Parallel Algorithms for Genomics (GenScale), Inria Rennes – Bretagne Atlantique, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-GESTION DES DONNÉES ET DE LA CONNAISSANCE (IRISA-D7), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Bretagne Sud (UBS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-École normale supérieure - Rennes (ENS Rennes)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Wageningen University and Research [Wageningen] (WUR), Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Université de Montpellier (UM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Evolution, génomes, comportement et écologie (EGCE), Institut de Recherche pour le Développement (IRD)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Recherche Génomique Info (URGI), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Escola Superior de Agricultura 'Luiz de Queiroz' (ESALQ), Universidade de São Paulo (USP), Department of Entomology [Urbana], University of Illinois System, Department of Terrestrial Ecology [Wageningen], Netherlands Institute of Ecology (NIOO-KNAW), ANR-12-ADAP-0001, Agence Nationale de la Recherche, Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Collège Doctoral, Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique (IMT Atlantique), Université de Rennes (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique (IMT Atlantique), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Universidade de São Paulo = University of São Paulo (USP), ANR-12-ADAP-0001,ABC - PaPoGen,Adaptation en lutte biologique - Génomique des populations de parasitoïdes(2012), Evolutionary Genetics, Development & Behaviour, Terrestrial Ecology (TE), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique (IMT Atlantique), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Rennes 1 (UR1), and Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique (IMT Atlantique)

Subjects
Genome evolution, food.ingredient, QH301-705.5, Bioinformatics, [SDV]Life Sciences [q-bio], Medicine (miscellaneous), macromolecular substances, Biology, Genome, Article, Evolutionary genetics, General Biochemistry, Genetics and Molecular Biology, Plan_S-Compliant-OA, Parasitoid wasp, 03 medical and health sciences, BIOS Applied Bioinformatics, 0302 clinical medicine, food, Bioinformatica, Life Science, Cotesia congregata, VÍRUS DE DNA, Biology (General), Laboratory of Entomology, Gene, 030304 developmental biology, 0303 health sciences, [SDV.GEN]Life Sciences [q-bio]/Genetics, Human evolutionary genetics, Chromosome, biology.organism_classification, PE&RC, Laboratorium voor Entomologie, Evolutionary biology, international, EPS, General Agricultural and Biological Sciences, Bracovirus, Entomology, 030217 neurology & neurosurgery
Abstract

Endogenous viruses form an important proportion of eukaryote genomes and a source of novel functions. How large DNA viruses integrated into a genome evolve when they confer a benefit to their host, however, remains unknown. Bracoviruses are essential for the parasitism success of parasitoid wasps, into whose genomes they integrated ~103 million years ago. Here we show, from the assembly of a parasitoid wasp genome at a chromosomal scale, that bracovirus genes colonized all ten chromosomes of Cotesia congregata. Most form clusters of genes involved in particle production or parasitism success. Genomic comparison with another wasp, Microplitis demolitor, revealed that these clusters were already established ~53 mya and thus belong to remarkably stable genomic structures, the architectures of which are evolutionary constrained. Transcriptomic analyses highlight temporal synchronization of viral gene expression without resulting in immune gene induction, suggesting that no conflicts remain between ancient symbiotic partners when benefits to them converge., Jeremy Gauthier et al. present the chromosome scale assembly of the genome of the parasitic wasp C. congregata and show that bracovirus genes have colonized all ten chromosomes. Comparison with genome scaffolds of another wasp reveals a striking stability of these regions over ~53 million years, suggesting strong evolutionary constraints.

Published
2021
Full Text
View/download PDF

9. Occurrence and Molecular Phylogeny of Honey Bee Viruses in Vespids

Author

Hongxia Zhao, Dahe Yang, Elisabeth A. Herniou, Yaojun Wu, Xuejian Jiang, Philippe Gayral, Xinling Wang, Eric Darrouzet, Sa Yang, Chunsheng Hou, Fei Li, Qingyun Diao, Yan-Yan Wu, Diane Bigot, Shuai Deng, Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), HiLIFE - Institute of Biotechnology [Helsinki] (BI), Helsinki Institute of Life Science (HiLIFE), University of Helsinki-University of Helsinki, Institute of Apicultural Research [Beijing, China], Chinese Academy of Agricultural Sciences (CAAS), China Agricultural University (CAU), and Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, 0301 basic medicine, viruses, [SDV]Life Sciences [q-bio], Zoology, Insect Viruses, complex mixtures, 01 natural sciences, Article, Virus, Animal Diseases, 03 medical and health sciences, IAPV, AmFV, Virology, Deformed wing virus, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Mite, Animals, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Phylogeny, DWV, honeybee viruses, biology, Phylogenetic tree, Vespidae, fungi, hornets, High-Throughput Nucleotide Sequencing, food and beverages, Honey bee, Bees, biology.organism_classification, Insect Vectors, 3. Good health, Phylogeography, 010602 entomology, 030104 developmental biology, Infectious Diseases, Varroa destructor, Molecular phylogenetics, behavior and behavior mechanisms
Abstract

Since the discovery that honey bee viruses play a role in colony decline, researchers have made major breakthroughs in understanding viral pathology and infection processes in honey bees. Work on virus transmission patterns and virus vectors, such as the mite Varroa destructor, has prompted intense efforts to manage honey bee health. However, little is known about the occurrence of honey bee viruses in bee predators, such as vespids. In this study, we characterized the occurrence of 11 honey bee viruses in five vespid species and one wasp from four provinces in China and two vespid species from four locations in France. The results showed that all the species from China carried certain honey bee viruses, notably Apis mellifera filamentous virus (AmFV), Deformed wing virus (DWV), and Israeli acute paralysis virus (IAPV), furthermore, in some vespid colonies, more than three different viruses were identified. In France, DWV was the most common virus, Sacbrood virus (SBV) and Black queen cell virus (BQCV) were observed in one and two samples, respectively. Phylogenetic analyses of IAPV and BQCV sequences indicated that most of the IAPV sequences belonged to a single group, while the BQCV sequences belonged to several groups. Additionally, our study is the first to detect Lake Sinai virus (LSV) in a hornet from China. Our findings can guide further research into the origin and transmission of honey bee viruses in Vespidae, a taxon of ecological, and potentially epidemiological, relevance.

Published
2020
Full Text
View/download PDF

10. Viruses in the Invasive Hornet Vespa velutina

Author

Damien Decante, Maxime Thomasson, Yves Le Conte, Cédric Alaux, Elisabeth A. Herniou, Diane Bigot, Anne Dalmon, Philippe Gayral, Christophe Klopp, Abeilles et Environnement (AE), Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU), Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours-Centre National de la Recherche Scientifique (CNRS), Unité de Biométrie et Intelligence Artificielle de Toulouse [Castanet-Tolosan] (UBIA), Institut National de la Recherche Agronomique (INRA)-Plateforme bioinformatique du GIS GENOTOUL - Génopole Toulouse Midi-Pyrénées, Dalmon, Anne, and Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, 0301 basic medicine, viruses, Wasps, Biological pest control, lcsh:QR1-502, [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy, 01 natural sciences, lcsh:Microbiology, invasive species, colonie d'abeilles, Deformed wing virus, frelon asiatique, vespa velutina, Predator, ComputingMilieux_MISCELLANEOUS, DWV, biology, [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], food and beverages, Bees, 3. Good health, honey bee viruses, Intestines, Vespidae, new viruses, Infectious Diseases, Biological Control Agents, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Dicistroviridae, behavior and behavior mechanisms, RNA, Viral, génome viral, ABPV, sud est france, RT-PCR, Insect Viruses, Genome, Viral, ALPV, BeeMLV, analyse phylogénétique, 010603 evolutionary biology, Virus, Article, 03 medical and health sciences, KBV, BQCV, Virology, séquençage, Animals, RNA Viruses, Vespa velutina, [SDV.GEN.GPO]Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE], Velutina, fungi, extraction d'arn, Honey bee, biology.organism_classification, vespidae, 030104 developmental biology, Metagenome, Introduced Species
Abstract

International audience; The Asian yellow-legged hornet Vespa velutina nigrithorax, a major predator of honeybees, is spreading in Europe in part due to a lack of efficient control methods. In this study, as a first step to identify biological control agents, we characterized viral RNA sequences present in asymptomatic or symptomatic hornets. Among 19 detected viruses, the honey bee virus Deformed wing virus-B was predominant in all the samples, particularly in muscles from the symptomatic hornet, suggesting a putative cause of the deformed wing symptom. Interestingly, two new viruses closely related to Acyrthosiphon pisum virus and Himetobi P virus and viruses typically associated with honey bees, Acute bee paralysis virus and Black queen cell virus, were detected in the brain and muscles, and may correspond to the circulation and possible replication forms of these viruses in the hornet. Aphid lethal paralysis virus, Bee Macula-like virus, and Moku virus, which are known to infect honey bees, were also identified in the gut virus metagenome of hornets. Therefore, our study underlined the urgent need to study the host range of these newly discovered viruses in hornets to determine whether they represent a new threat for honey bees or a hope for the biocontrol of V. velutina.

Published
2019
Full Text
View/download PDF

11. The invasive hornet Vespa velutina carries both honey bee viruses and new viruses

Author

Anne BONJOUR-DALMON, Philippe Gayral, Damien Decante, Christophe Klopp, Diane Bigot, Maxime Thomasson, Elisabeth Herniou, Cédric Alaux, Yves Le Conte, Abeilles & Environnement (UR 406 ), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), UMT PrADE, Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut Technique et Scientifique de l'Apiculture et de la Pollinisation (ITSAP-Institut de l'Abeille), Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRA), Institut National de la Recherche Agronomique (INRA), Martin-Luther-University Halle-Wittenberg, and Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [INFO]Computer Science [cs], [MATH]Mathematics [math]
Abstract

National audience; Since its introduction in 2004 in France the asian yellow-legged hornet Vespa velutina nigrithorax is now largely spreading into Europe, by lack of efficient control methods. In this context, looking for natural hornet pathogens could be useful to develop biological control agents against this invasive species. Therefore, we investigated all RNA viral sequances detected from hornets samples caught in Southern France, in order to characterize any virus from asymptomatic or symptomatic hornets differentiating various dissected parts (brain, muscle, abdomen). Among almost twenty viruses detected in three RNA sequenced hornets, Deformed wing virus B was shown to be predominant in all samples, and much higher in the muscle from symptomatic individual, suggesting a putative cause of the deformed wings symptoms. Complete genomes of Acute bee paralysis virus (ABPV, Dicistroviridae, Aparavirus) and Black queen cell virus ( BQCV, Dicistroviridae, Triatovirus) were found in the intestine, and multiplex PCR detection confirmed these viruses were present in different dissected parts of several hornets. Five other viruses known to infect honeybees were also partially sequenced. Eleven other insect viruses were detected in hornets, most of them belonging to picornavirales order, but some of them resulted as new species and possibly new genus. Nine of them were detected only in the intestine transcriptome, and not in brain or muscle tissues, suggesting they could originate from infected preys ingested by hornet, and not by infected hornets thenselves and thus giving indirect data about hornet diet. The transmission routes from or to V. velutina have to be investigated to better assess the impact they may have on virus dissemination in European honeybee populations. Our study underlines the urgent need to study the host range of these original newly discovered virus that we described in hornets to evaluate if they can represent a new threat for the honeybees or a hope for the biocontrol of V. velutina.

Published
2019

12. A56 Visualization of recombination in deformed wing virus infecting bees

Author

Diane Bigot, Andreas Gogol-Döring, Robert J. Paxton, and Peter Koch

Subjects
Physics, biology, Evolutionary biology, Virology, Deformed wing virus, fungi, behavior and behavior mechanisms, Abstract Overview, biology.organism_classification, Microbiology, Recombination, Visualization
Abstract

Honey bees suffer increasing colony mortality worldwide, partially caused by the spread of viral pathogens. Among these pathogens, deformed wing virus (DWV) is one of the major, widespread viruses of honey bees resulting in wing deformities and weakening colonies. DWV can be found in honey bees, bumble bees, and other wild bees as three major genotypes named DWV-A, -B (also named Varroa destructor virus 1), and -C. Various recombinants of DWV-A and -B have been previously found in honey bees, some of which have been suggested to have higher virulence over non-recombinant, parental virus. In most of these cases, recombinants were only shown as consensus sequences from previous assemblies and alignments and may not reflect the biological reality of all variants present within a host bee. It is therefore important to build a method of recombinant detection and quantification within mixed infections in single-host individuals, including both parental and various recombinant genomes, so as to evaluate the relevance of recombinants for viral genome evolution and the impact on hosts. Here, we propose to visualize and quantify these recombinants using next-generation sequencing data to better understand how these genomes evolve within bees. Our method will be performed directly from raw sequence reads from various datasets (including field and lab experiments as well as screening of public databases) in order to obtain an overview of DWV recombination in various in vivo and in vitro conditions. Recombination of viral genomes is a key point for virus evolution. The detection and quantification of recombination will facilitate analysis of the determinants of recombination and help in understanding the routes by which new viral variants emerge. The emergence of new (more virulent) recombinant viruses can result from acquisition of new capabilities, such as escape from host immunity or increased transmission rates. Recombination can also lead to adaptation to new environments and new hosts by a change in cell tropism, allowing cross-species transmission, which may be particularly relevant for bumble bees and wild bees infected by honey bee-derived DWV.

Published
2019
Full Text
View/download PDF

13. Discovery of Culex pipiens associated tunisia virus: a new ssRNA(+) virus representing a new insect associated virus family

Author

Philippe Gayral, Diane Bigot, Célestine M. Atyame, Mylène Weill, Elisabeth A. Herniou, Fabienne Justy, Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226, and Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Culex pipiens mosquitoes, Plasmodium, RNA virus, viruses, 030106 microbiology, Microbiology, Genome, Deep sequencing, Virus, Virgaviridae, 03 medical and health sciences, Virology, Plant virus, Culex pipiens, ORFS, Genetics, virus discovery, biology, biology.organism_classification, 030104 developmental biology, [SDE]Environmental Sciences, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Research Article, CpATV
Abstract

International audience; In the global context of arboviral emergence, deep sequencing unlocks the discovery of new mosquito-borne viruses. Mosquitoes of the species Culex pipiens, C. torrentium, and C. hortensis were sampled from 22 locations worldwide for transcriptomic analyses. A virus discovery pipeline was used to analyze the dataset of 0.7 billion reads comprising 22 individual transcriptomes. Two closely related 6.8 kb viral genomes were identified in C. pipiens and named as Culex pipiens associated tunisia virus (CpATV) strains Ayed and Jedaida. The CpATV genome contained four ORFs. ORF1 possessed helicase and RNA-dependent RNA polymerase (RdRp) domains related to new viral sequences recently found mainly in dipterans. ORF2 and 4 contained a capsid protein domain showing strong homology with Virgaviridae plant viruses. ORF3 displayed similarities with eukaryotic Rhoptry domain and a merozoite surface protein (MSP7) domain only found in mosquito-transmitted Plasmodium, suggesting possible interactions between CpATV and vertebrate cells. Estimation of a strong purifying selection exerted on each ORFs and the presence of a polymorphism maintained in the coding region of ORF3 suggested that both CpATV sequences are genuine functional viruses. CpATV is part of an entirely new and highly diversified group of viruses recently found in insects, and that bears the genomic hallmarks of a new viral family.

Published
2018
Full Text
View/download PDF

14. The discovery of Halictivirus resolves the Sinaivirus phylogeny

Author

Chunsheng Hou, Lucy A. Weinert, Manon Romary, Diane Bigot, Michèle Germain, Bronwen Roy, Elisabeth A. Herniou, Qingyun Diao, Shuai Deng, Philippe Gayral, James M. Cook, Anne Dalmon, Weinert, Lucy [0000-0002-9279-6012], Apollo - University of Cambridge Repository, Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours-Centre National de la Recherche Scientifique (CNRS), Abeilles et Environnement (AE), Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU), The University of Western Australia (UWA), Institute of Apicultural Research [Beijing, China], Chinese Academy of Agricultural Sciences (CAAS), Department of Veterinary Medicine, University of Cambridge, Cambridge, UK, Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226, European Project: 232971,EC:FP7:ERC,ERC-2008-AdG,POPPHYL(2009), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), University of Cambridge [UK] (CAM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Aphaenogaster, viruses, 030106 microbiology, Insect Viruses, [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy, 03 medical and health sciences, RNA virus discovery, Viral Proteins, wild bee, Halictus scabiosae, Phylogenetics, Virology, Botany, Animals, HsAV, ComputingMilieux_MISCELLANEOUS, Phylogeny, [SDV.GEN.GPO]Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE], LSV, biology, Phylogenetic tree, Ants, Halictus, [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], Messor barbarus, fungi, Messor, food and beverages, Genetic Variation, Honey bee, Sequence Analysis, DNA, Bees, biology.organism_classification, 030104 developmental biology, Evolutionary biology, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, behavior and behavior mechanisms, hymenoptera
Abstract

International audience; By providing pollination services, bees are among the most important insects, both in ecological and economical terms. Combined next-generation and classical sequencing approaches were applied to discover and study new insect viruses potentially harmful to bees. A bioinformatics virus discovery pipeline was used on individual Illumina transcriptomes of 13 wild bees from three species from the genus Halictus and 30 ants from six species of the genera Messor and Aphaenogaster. This allowed the discovery and description of three sequences of a new virus termed Halictus scabiosae Adlikon virus (HsAV). Phylogenetic analyses of ORF1, RNA-dependent RNA-polymerase (RdRp) and capsid genes showed that HsAV is closely related to (+)ssRNA viruses of the unassigned Sinaivirus genus but distant enough to belong to a different new genus we called Halictivirus. In addition, our study of ant transcriptomes revealed the first four sinaivirus sequences from ants (Messor barbarus, M. capitatus and M. concolor). Maximum likelihood phylogenetic analyses were performed on a 594 nt fragment of the ORF1/RdRp region from 84 sinaivirus sequences, including 31 new Lake Sinai viruses (LSVs) from honey bees collected in five countries across the globe and the four ant viral sequences. The phylogeny revealed four main clades potentially representing different viral species infecting honey bees. Moreover, the ant viruses belonged to the LSV4 clade, suggesting a possible cross-species transmission between bees and ants. Lastly, wide honey bee screening showed that all four LSV clades have worldwide distributions with no obvious geographical segregation.

Published
2017
Full Text
View/download PDF

15. Diversité et évolution du Lake Sinaï Virus (LSV) chez les abeilles domestiques et les hyménoptères sauvages

Author

Anne BONJOUR-DALMON, Diane Bigot, Elisabeth Herniou, Nicolas Galtier, Philippe Gayral, Abeilles & Environnement (UR 406 ), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Francois Rabelais [Tours], Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

16. Comparative Analysis of Transcriptomes from Secondary Reproductives of Three Reticulitermes Termite Species

Author

Lucy A. Weinert, Diane Bigot, Nicolas Galtier, Franck Dedeine, Thibaut Josse, Vincent Cahais, Marion Ballenghien, Philippe Gayral, Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Université de Tours-Centre National de la Recherche Scientifique (CNRS), École pratique des hautes études (EPHE), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226

Subjects
Gene prediction, Population genetics, lcsh:Medicine, Isoptera, [SDV.BID]Life Sciences [q-bio]/Biodiversity, Transcriptome, Reticulitermes, Animals, lcsh:Science, Gene, Genetics, Multidisciplinary, biology, Reproduction, lcsh:R, Computational Biology, Interspecific competition, biology.organism_classification, Eusociality, Genetics, Population, Evolutionary biology, Insect Proteins, Female, lcsh:Q, Orthologous Gene, Research Article
Abstract

International audience; Termites are eusocial insects related to cockroaches that feed on lignocellulose. These insects are key species in ecosystems since they recycle a large amount of nutrients but also are pests, exerting major economic impacts. Knowledge on the molecular pathways underlying reproduction, caste differentiation or lignocellulose digestion would largely benefit from additional transcriptomic data. This study focused on transcriptomes of secondary reproductive females (nymphoid neotenics). Thirteen transcriptomes were used: 10 of Reti-culitermes flavipes and R. grassei sequenced from a previous study, and two transcrip-tomes of R. lucifugus sequenced for the present study. After transcriptome assembly and read mapping, we examined interspecific variations of genes expressed by termites or gut microorganisms. A total of 18,323 orthologous gene clusters were detected. Functional annotation and taxonomic assignment were performed on a total of 41,287 predicted con-tigs in the three termite species. Between the termite species studied, functional categories of genes were comparable. Gene ontology (GO) terms analysis allowed the discovery of 9 cellulases and a total of 79 contigs potentially involved in 11 enzymatic activities used in wood metabolism. Altogether, results of this study illustrate the strong potential for the use of comparative interspecific transcriptomes, representing a complete resource for future studies including differentially expressed genes between castes or SNP analysis for population genetics.

Published
2015
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results