1. Bacillus cytotoxicus sp. nov. is a novel thermotolerant species of the Bacillus cereus Group occasionally associated with food poisoning
- Author
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Paul De Vos, Marie-Laure De Buyser, Matthias Contzen, Benoît de Sarrau, Didier Lereclus, Annette Fagerlund, Gilles Lamberet, Nathalie Galleron, Per Einar Granum, Christophe Nguyen-The, Marie-Hélène Guinebretière, Alexei Sorokin, Sandrine Auger, Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, MetaGénoPolis, Institut National de la Recherche Agronomique (INRA), Unité Caractérisation et Epidémiologie Bactérienne, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), Department Biochemistry and Microbiology, Laboratory for Microbiology, Ghent University [Belgium] (UGENT), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), MetaGenoPolis, Universiteit Gent = Ghent University [Belgium] (UGENT), French National Research Agency [ANR-05-PNRA-013], and INRA's MICA department
- Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences ,DNA, Bacterial ,Sequence analysis ,Bacillus cereus ,Bacillus ,Microbiology ,Foodborne Diseases ,03 medical and health sciences ,Phylogenetics ,RNA, Ribosomal, 16S ,Ecology, Evolution, Behavior and Systematics ,Phylogeny ,030304 developmental biology ,Bacillus (shape) ,0303 health sciences ,Base Composition ,biology ,030306 microbiology ,fungi ,Fatty Acids ,Nucleic Acid Hybridization ,General Medicine ,Sequence Analysis, DNA ,Ribosomal RNA ,biology.organism_classification ,16S ribosomal RNA ,Bacterial Typing Techniques ,Cereus ,Multilocus sequence typing ,France ,Multilocus Sequence Typing - Abstract
An aerobic endospore-forming bacillus (NVH 391-98T) was isolated during a severe food poisoning outbreak in France in 1998, and four other similar strains have since been isolated, also mostly from food poisoning cases. Based on 16S rRNA gene sequence similarity, these strains were shown to belong to the Bacillus cereus Group (over 97 % similarity with the current Group species) and phylogenetic distance from other validly described species of the genus Bacillus was less than 95 %. Based on 16S rRNA gene sequence similarity and MLST data, these novel strains were shown to form a robust and well-separated cluster in the B. cereus Group, and constituted the most distant cluster from species of this Group. Major fatty acids (iso-C15 : 0, C16 : 0, iso-C17 : 0, anteiso-C15 : 0, iso-C16 : 0, iso-C13 : 0) supported the affiliation of these strains to the genus Bacillus , and more specifically to the B. cereus Group. NVH 391-98T taxon was more specifically characterized by an abundance of iso-C15 : 0 and low amounts of iso-C13 : 0 compared with other members of the B. cereus Group. Genome similarity together with DNA–DNA hybridization values and physiological and biochemical tests made it possible to genotypically and phenotypically differentiate NVH 391-98T taxon from the six current B. cereus Group species. NVH 391-98T therefore represents a novel species, for which the name Bacillus cytotoxicus sp. nov. is proposed, with the type strain NVH 391-98T ( = DSM 22905T = CIP 110041T).
- Published
- 2012
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