Back to Search Start Over

Bacillus cytotoxicus sp. nov. is a novel thermotolerant species of the Bacillus cereus Group occasionally associated with food poisoning

Bacillus cytotoxicus sp. nov. is a novel thermotolerant species of the Bacillus cereus Group occasionally associated with food poisoning

Authors :
Paul De Vos
Marie-Laure De Buyser
Matthias Contzen
Benoît de Sarrau
Didier Lereclus
Annette Fagerlund
Gilles Lamberet
Nathalie Galleron
Per Einar Granum
Christophe Nguyen-The
Marie-Hélène Guinebretière
Alexei Sorokin
Sandrine Auger
Sécurité et Qualité des Produits d'Origine Végétale (SQPOV)
Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU)
MICrobiologie de l'ALImentation au Service de la Santé (MICALIS)
Institut National de la Recherche Agronomique (INRA)-AgroParisTech
MetaGénoPolis
Institut National de la Recherche Agronomique (INRA)
Unité Caractérisation et Epidémiologie Bactérienne
Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)
Department of Food Safety and Infection Biology
Norwegian School of Veterinary Science
Department of Chemistry, Biotechnology and Food Science
Norwegian University of Life Sciences (NMBU)
Department Biochemistry and Microbiology, Laboratory for Microbiology
Ghent University [Belgium] (UGENT)
Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
MetaGenoPolis
Universiteit Gent = Ghent University [Belgium] (UGENT)
French National Research Agency [ANR-05-PNRA-013]
INRA's MICA department
Source :
International Journal of Systematic and Evolutionary Microbiology, International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2013, 63, pp.31-40. ⟨10.1099/ijs.0.030627-0⟩
Publication Year :
2012

Abstract

An aerobic endospore-forming bacillus (NVH 391-98T) was isolated during a severe food poisoning outbreak in France in 1998, and four other similar strains have since been isolated, also mostly from food poisoning cases. Based on 16S rRNA gene sequence similarity, these strains were shown to belong to the Bacillus cereus Group (over 97 % similarity with the current Group species) and phylogenetic distance from other validly described species of the genus Bacillus was less than 95 %. Based on 16S rRNA gene sequence similarity and MLST data, these novel strains were shown to form a robust and well-separated cluster in the B. cereus Group, and constituted the most distant cluster from species of this Group. Major fatty acids (iso-C15 : 0, C16 : 0, iso-C17 : 0, anteiso-C15 : 0, iso-C16 : 0, iso-C13 : 0) supported the affiliation of these strains to the genus Bacillus , and more specifically to the B. cereus Group. NVH 391-98T taxon was more specifically characterized by an abundance of iso-C15 : 0 and low amounts of iso-C13 : 0 compared with other members of the B. cereus Group. Genome similarity together with DNA–DNA hybridization values and physiological and biochemical tests made it possible to genotypically and phenotypically differentiate NVH 391-98T taxon from the six current B. cereus Group species. NVH 391-98T therefore represents a novel species, for which the name Bacillus cytotoxicus sp. nov. is proposed, with the type strain NVH 391-98T ( = DSM 22905T = CIP 110041T).

Details

ISSN :
14665034 and 14665026
Volume :
63
Issue :
Pt 1
Database :
OpenAIRE
Journal :
International journal of systematic and evolutionary microbiology
Accession number :
edsair.doi.dedup.....90b99b56c1b741788434d0150fb327c7
Full Text :
https://doi.org/10.1099/ijs.0.030627-0⟩