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Bacillus cytotoxicus sp. nov. is a novel thermotolerant species of the Bacillus cereus Group occasionally associated with food poisoning
Bacillus cytotoxicus sp. nov. is a novel thermotolerant species of the Bacillus cereus Group occasionally associated with food poisoning
- Source :
- International Journal of Systematic and Evolutionary Microbiology, International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2013, 63, pp.31-40. ⟨10.1099/ijs.0.030627-0⟩
- Publication Year :
- 2012
-
Abstract
- An aerobic endospore-forming bacillus (NVH 391-98T) was isolated during a severe food poisoning outbreak in France in 1998, and four other similar strains have since been isolated, also mostly from food poisoning cases. Based on 16S rRNA gene sequence similarity, these strains were shown to belong to the Bacillus cereus Group (over 97 % similarity with the current Group species) and phylogenetic distance from other validly described species of the genus Bacillus was less than 95 %. Based on 16S rRNA gene sequence similarity and MLST data, these novel strains were shown to form a robust and well-separated cluster in the B. cereus Group, and constituted the most distant cluster from species of this Group. Major fatty acids (iso-C15 : 0, C16 : 0, iso-C17 : 0, anteiso-C15 : 0, iso-C16 : 0, iso-C13 : 0) supported the affiliation of these strains to the genus Bacillus , and more specifically to the B. cereus Group. NVH 391-98T taxon was more specifically characterized by an abundance of iso-C15 : 0 and low amounts of iso-C13 : 0 compared with other members of the B. cereus Group. Genome similarity together with DNA–DNA hybridization values and physiological and biochemical tests made it possible to genotypically and phenotypically differentiate NVH 391-98T taxon from the six current B. cereus Group species. NVH 391-98T therefore represents a novel species, for which the name Bacillus cytotoxicus sp. nov. is proposed, with the type strain NVH 391-98T ( = DSM 22905T = CIP 110041T).
- Subjects :
- [SDV.SA]Life Sciences [q-bio]/Agricultural sciences
DNA, Bacterial
Sequence analysis
Bacillus cereus
Bacillus
Microbiology
Foodborne Diseases
03 medical and health sciences
Phylogenetics
RNA, Ribosomal, 16S
Ecology, Evolution, Behavior and Systematics
Phylogeny
030304 developmental biology
Bacillus (shape)
0303 health sciences
Base Composition
biology
030306 microbiology
fungi
Fatty Acids
Nucleic Acid Hybridization
General Medicine
Sequence Analysis, DNA
Ribosomal RNA
biology.organism_classification
16S ribosomal RNA
Bacterial Typing Techniques
Cereus
Multilocus sequence typing
France
Multilocus Sequence Typing
Subjects
Details
- ISSN :
- 14665034 and 14665026
- Volume :
- 63
- Issue :
- Pt 1
- Database :
- OpenAIRE
- Journal :
- International journal of systematic and evolutionary microbiology
- Accession number :
- edsair.doi.dedup.....90b99b56c1b741788434d0150fb327c7
- Full Text :
- https://doi.org/10.1099/ijs.0.030627-0⟩