95 results on '"Dellanoce C"'
Search Results
2. Glutamatergic dysfunction, neuroplasticity, and redox status in the peripheral blood of patients with motor conversion disorders (functional movement disorders): a first step towards potential biomarkers discovery
- Author
-
Demartini, B, Nistico', V, Benayoun, C, Cigognini, A, Ferrucci, R, Vezzoli, A, Dellanoce, C, Gambini, O, Priori, A, Mrakic-Sposta, S, Demartini B., Nistico' V., Benayoun C., Cigognini A. C., Ferrucci R., Vezzoli A., Dellanoce C., Gambini O., Priori A., Mrakic-Sposta S., Demartini, B, Nistico', V, Benayoun, C, Cigognini, A, Ferrucci, R, Vezzoli, A, Dellanoce, C, Gambini, O, Priori, A, Mrakic-Sposta, S, Demartini B., Nistico' V., Benayoun C., Cigognini A. C., Ferrucci R., Vezzoli A., Dellanoce C., Gambini O., Priori A., and Mrakic-Sposta S.
- Abstract
Functional movement disorders (FMD) are characterized by the presence of neurological symptoms that cannot be explained by typical neurological diseases or other medical conditions. First evidence showed that, compared to healthy controls (CTR), FMD patients presented increased levels of glutamate+glutamine in the anterior cingulate cortex/medial prefrontal cortex, and decreased levels of glutamate in the cerebrospinal fluid, suggesting that a glutamatergic dysfunction might play a role in FMD pathophysiology. In this study, 12 FMD patients and 20 CTR were recruited and underwent venous blood sampling and urine collection: levels of glutamate, BDNF, dopamine, oxidative stress, creatinine, neopterin, and uric acid were analyzed. Participants also underwent a psychometric assessment investigating depression, anxiety, and alexithymia. We found that levels of glutamate, BDNF, and dopamine were significantly lower in the blood of FMD patients than CTR. Glutamate and dopamine levels were positively associated with levels of alexithymia. Our findings give further evidence that glutamatergic dysfunction might be involved in the pathophysiology of FMD, possibly representing a biomarker of disease; moreover, since glutamatergic and dopaminergic systems are closely interconnected, our results might have a relevance in terms of treatment options for FMD patients.
- Published
- 2023
3. Understanding complex effects on clinical outcomes and biomarker activities of food supplementation in heavy smokers: an approach using bayesian networks.
- Author
-
Vigna, L., Gori, F., Bamonti, F., Tomaino, L., Zaccagna, C.A., Cighetti, G., Dellanoce, C., and Gregori, D.
- Subjects
BAYESIAN analysis ,MONTE Carlo method ,CARDIOVASCULAR diseases risk factors ,TREATMENT effectiveness ,BIOMARKERS ,BERRIES ,ENRICHED foods - Abstract
BACKGROUND: Antioxidant administration can significantly alleviate smoking-mediated oxidative stress. In our previous research, using a concentrated mixed juice powder led to significant reduction in oxidative status markers and improved lung function among smokers. OBJECTIVE: To compare the effectiveness of different antioxidant formulations of the retail product Juice Plus+
® on oxidative status and lung function in healthy smokers via a a Bayesian Network (BN) analysis. METHODS: At baseline and after 3 months' supplementation, pulmonary function, cardiovascular risk factors, and oxidative status were assessed in 101 smokers, randomized into three groups: placebo (P), fruit/vegetable (FV), and fruit/vegetable/berry (FVB) group. BN analysis was performed via Monte Carlo simulations. Statistical significance was assigned as p < 0.05. RESULTS: No significant interaction was observed in P (network score –101.4, p = ns), whereas overall fit significantly improved in both FV (network score –185.7, p = 0.01) and FVB (–293.7, p = 0.003). Moreover, P group's respiratory parameters appeared unrelated to the biochemical structure, whereas those of the FV and, to a greater extent, FVB groups were definitely related to it CONCLUSIONS: BN analysis demonstrated that the FV and FVB formulations contain nutraceutical compounds capable of synergistically acting and improving antioxidant modulation and respiratory function in smoking subjects [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
4. Low drug levels and thrombotic complications in high‐risk atrial fibrillation patients treated with direct oral anticoagulants
- Author
-
Testa, S., Paoletti, O., Legnani, C., Dellanoce, C., Antonucci, E., Cosmi, B., Pengo, V., Poli, D., Morandini, R., Testa, R., Tripodi, A., and Palareti, G.
- Published
- 2018
- Full Text
- View/download PDF
5. Dabigatran but not rivaroxaban or apixaban reduces fibrinolytic resistance in patients with atrial fibrillation: OR065
- Author
-
Ammollo, C T, Semeraro, F, Incampo, F, Dellanoce, C, Paoletti, O, Testa, S, and Colucci, M
- Published
- 2015
6. A global quality control system to check PT-INR portable monitor for Antivitamin K antagonists
- Author
-
TESTA, S., PAOLETTI, O., BASSI, L., DELLANOCE, C., MORANDINI, R., and LIPPI, G.
- Published
- 2015
- Full Text
- View/download PDF
7. Acute effects of different degrees of ultra-endurance exercise on systemic inflammatory responses
- Author
-
Comassi, M., Vitolo, E., Pratali, L., Del Turco, S., Dellanoce, C., Rossi, C., Santini, E., and Solini, A.
- Published
- 2015
- Full Text
- View/download PDF
8. Urinary neopterin, a new marker of the neuroinflammatory status in amyotrophic lateral sclerosis
- Author
-
Lunetta, C, Lizio, A, Gerardi, F, Tarlarini, C, Filippi, M, Riva, N, Tremolizzo, L, Diamanti, S, Dellanoce, C, Mosca, L, Sansone, V, Campolo, J, Lunetta, Christian, Lizio, Andrea, Gerardi, Francesca, Tarlarini, Claudia, Filippi, Massimo, Riva, Nilo, Tremolizzo, Lucio, Diamanti, Susanna, Dellanoce, Cinzia Carla, Mosca, Lorena, Sansone, Valeria Ada, Campolo, Jonica, Lunetta, C, Lizio, A, Gerardi, F, Tarlarini, C, Filippi, M, Riva, N, Tremolizzo, L, Diamanti, S, Dellanoce, C, Mosca, L, Sansone, V, Campolo, J, Lunetta, Christian, Lizio, Andrea, Gerardi, Francesca, Tarlarini, Claudia, Filippi, Massimo, Riva, Nilo, Tremolizzo, Lucio, Diamanti, Susanna, Dellanoce, Cinzia Carla, Mosca, Lorena, Sansone, Valeria Ada, and Campolo, Jonica
- Abstract
Objective: To comprehensively assess whether neopterin in urine could be a candidate biomarker for determining the neuroinflammatory status in ALS. Methods: We performed an observational, cross-sectional study in 81 pALS, 68 age- and sex-comparable healthy controls (HC), 14 patients affected by MS and 24 OND patients. ALS patients underwent a neurological evaluation to assess the global functional status evaluated by Amyotrophic Lateral Sclerosis Functional Rating Scale-Revised (ALSFRS-R) and the disease progression rate. Urinary neopterin concentrations were determined by high-performance liquid chromatography method and were recorded at the time of first examination to assess their effect on disease severity and survival. Results: Urinary neopterin was significantly higher in pALS (263.90 [198.71-474.90]) compared to MS (155.28 [131.74-190.38], p = < .001), OND patients (205.60 [158.96-299.41], p = 0.04) and HC (169.55 [134.91-226.10], p < .001). Moreover, a significant negative correlation was found between neopterin level and the severity of symptoms evaluated by ALSFRS-R total score (r = - 0.46, p < .001) and its subscores (bulbar r = - 0.34, p = 0.002; motor r = - 0.33, p = 0.003; respiratory r = - 0.53, p < .001), also adjusting for the effect of sex, site of onset, age at evaluation and time from onset to evaluation. Conclusions: Our finding indicates that urine neopterin is elevated in ALS, emphasizing the role of the cell-mediated inflammation in the disease. Moreover, whether confirmed in further studies, our results will underline the neopterin's potential use as non-invasive clinical biomarker of ALS, to discriminate patients possibly candidates to clinical interventions aimed to interfere the neuroinflammatory processes.
- Published
- 2020
9. A personalized system to enhance INR monitoring: PO 268
- Author
-
Paoletti, O, Linder, M, Zimmermann, A, Bassi, L, Delpero, N, Valdes, R, Dellanoce, C, and Testa, S
- Published
- 2013
10. Carbetocin increases thrombin generation after cesarean section: PB 2.66–4
- Author
-
Testa, S, Paoletti, O, Dellanoce, C, Bassi, L, Stramezzi, M, Spotti, E, Mammoliti, D, and Riccardi, A
- Published
- 2013
11. Thrombin generation test: a new and simplified expression of results: PB 2.53–4
- Author
-
Dellanoce, C, Paoletti, O, Bassi, L, and Testa, S
- Published
- 2013
12. INR portable monitors: efficacy of a quality control system: PB 2.53–2
- Author
-
Paoletti, O, Bassi, L, Cancellieri, E, Dellanoce, C, Zimmermann, A, Delpero, N, and Testa, S
- Published
- 2013
13. Comparison between different point of care systems for PT INR testing: PB 1.54–3
- Author
-
Bassi, L, Paoletti, O, Cancellieri, E, Damiolini, E, Cogrossi, A, Dellanoce, C, Zimmermann, A, and Testa, S
- Published
- 2013
14. Effects of acute and sub-acute hypobaric hypoxia on oxidative stress: a field study in the Alps
- Author
-
Mrakic-Sposta, S., primary, Gussoni, M., additional, Dellanoce, C., additional, Marzorati, M., additional, Montorsi, M., additional, Rasica, L., additional, Pratali, L., additional, D’Angelo, G., additional, Martinelli, M., additional, Bastiani, L., additional, Di Natale, L., additional, and Vezzoli, A., additional
- Published
- 2020
- Full Text
- View/download PDF
15. Oxidative stress as a biomarker for monitoring treated celiac disease article
- Author
-
Moretti S., Mrakic-Sposta S., Roncoroni L., Vezzoli A., Dellanoce C., Monguzzi E., Branchi F., Ferretti F., Lombardo V., Doneda L., Scricciolo A., and Elli L.
- Subjects
celiac disease ,Oxidative stress - Abstract
Introduction: High levels of reactive oxygen species (ROS) and impaired antioxidant defense systems lead to oxidative stress (OxS) and tissue injury in different intestinal and extra intestinal conditions, including celiac disease (CD). The aim of the present study was to investigate the role and potential use of ROS and other biomarkers of OxS in the clinical management of CD. Methods: We collected duodenal specimens and blood samples from naïve patients (N-CD), patients on a gluten free diet (GFD) including responders (CD-GFD) and non-responders (NRCD). We measured plasmatic ROS production (electron paramagnetic resonance, EPR), lipid peroxidation (thiobarbituric acid-reactive substances, TBARS), protein oxidation (protein carbonyl, PC), total antioxidant capacity (TAC), nitric oxides and glutathione (GSH) in erythrocytes. Results: Fifty-four patients affected by CD were enrolled (17 N-CD, 18 CD-GFD and 19 NRCD; 44 F; age 44 ± 13 years). A significant increase of plasmatic OxS biomarkers (ROS, peroxidated lipids, oxidized proteins, and nitrate concentrations) and decrease of antioxidant species (TAC and GSH levels) were found in NRCD and N-CD compared to CD-GFD. Comparably, a significant direct relationship between the severity of duodenal atrophy, ROS production rates and TBARS was found; conversely, TAC and GSH presented an inverse correlation. Discussion: OxS is involved in CD tissue damage and correlates with the degree of duodenal atrophy. These findings suggest the possible role of OxS biomarkers as indicators of CD activity during the clinical follow-up.
- Published
- 2018
16. Medium-term effect of sublingual l-glutathione supplementation on flow-mediated dilation in subjects with cardiovascular risk factors
- Author
-
Campolo, J, Bernardi, S, Cozzi, L, Rocchiccioli, S, Dellanoce, C, Cecchettini, A, Tonini, A, Parolini, M, De Chiara, B, Micheloni, G, Pelosi, G, Passino, C, Giannattasio, C, Parodi, O, Campolo, J, Bernardi, S, Cozzi, L, Rocchiccioli, S, Dellanoce, C, Cecchettini, A, Tonini, A, Parolini, M, De Chiara, B, Micheloni, G, Pelosi, G, Passino, C, Giannattasio, C, and Parodi, O
- Abstract
Objective Supplementation of glutathione (GSH) may be a positive strategy to improve the endogenous antioxidant defense required to counteract many acute and chronic diseases. However, the efficacy of GSH treatment seems to be closely related to type of administration, degree of absorption, and increase of its concentrations. The aim of this study was to test a new sublingual formulation of L-GSH, which enters directly the systemic circulation, to assess its efficacy on circulating biochemical markers of hepatic metabolism, lipid profile, and oxidative stress and on peripheral vascular function compared with placebo in patients with cardiovascular risk factors (CVRF). Methods We enrolled 16 healthy men with CVRF in a double-blinded, randomized placebo-controlled crossover study. At each visit, blood samples were collected for biochemistry analyses and peripheral endothelial function (reactive hyperemia index [RHI]) and stiffness were measured by Endo-PAT2000. Results In the overall population, a decrease in total and low-density lipoprotein cholesterol was highlighted after L-GSH supplementation compared with placebo (P = 0.023 and P = 0.04, respectively). On the contrary, no difference was observed in RHI and oxidative stress markers between L-GSH and placebo in the study population. However, seven participants with baseline abnormal RHI (≤1.67) compared with those with normal RHI showed a significant reduction of arterial stiffness after L-GSH administration, (P = 0.007 and P = 0.037, respectively). Conclusions Supplementation of L-GSH compared with placebo influences the lipid profile of patients with CVRF. Sublingual L-GSH may represent a valid prevention of vascular damage in patients with CVRF and endothelial dysfunction.
- Published
- 2017
17. Comparison of five specific assays for determination of dabigatran plasma concentrations in patients enrolled in the START‐Laboratory Register.
- Author
-
Cini, M., Legnani, C., Cosmi, B., Testa, S., Dellanoce, C., Paoletti, O., Marcucci, R., Poli, D., Paniccia, R., Pengo, V., Tripodi, A., Palareti, G., and for the START‐Laboratory Register
- Subjects
BENZIMIDAZOLES ,BIOLOGICAL assay ,COMPARATIVE studies ,CLINICAL pathology ,REPORTING of diseases ,MEDICAL cooperation ,PYRIDINE ,QUALITY control ,RELIABILITY (Personality trait) ,RESEARCH ,RESEARCH evaluation ,ACCURACY - Abstract
Abstract: Introduction: Several specific assays are commercially available to determine dabigatran anticoagulant activity. Aims of this multicenter and multiplatform study were to compare five methods for dabigatran measurement and investigate their performances in the low concentration range. Methods: Dabigatran levels were analyzed in 295 plasma samples from patients enrolled in the START‐Laboratory Register by the following methods using dedicated calibrators and controls: STA‐ECA II (Diagnostica Stago), standard and low range Hemoclot Thrombin Inhibitors (Hyphen BioMed), Direct Thrombin Inhibitor Assay (Instrumentation Laboratory), Direct Thrombin Inhibitor Assay (Siemens), Technoclot DTI (Technoclone). Results: Methods showed variable agreement with the Hemoclot Thrombin Inhibitors assay used as reference test, with modest under‐ or overestimations (Bland‐Altman bias from −17.3 to 4.0 ng/mL). Limits of detection and quantification varied depending on the assay (4‐52 and 7‐82 ng/mL, respectively). Between‐run precision and accuracy were good for all methods for both quality control levels. Assay's repeatability assessed at very low dabigatran concentrations (from 10 to 60 ng/mL) was also acceptable, variability generally increased at lower drug levels. Conclusion: The five dabigatran‐specific assays evaluated in this study provided reliable assessment of dabigatran plasma levels, although showing different performances. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
18. A global quality control system to check PT-INR portable monitor for Antivitamin K antagonists
- Author
-
Testa, S., primary, Paoletti, O., additional, Bassi, L., additional, Dellanoce, C., additional, Morandini, R., additional, and Lippi, G., additional
- Published
- 2014
- Full Text
- View/download PDF
19. EFFECTIVENESS OF A FOOD SUPPLEMENT (RIDUCOL) IN IMPROVING THE LIPID PANEL IN DYSLIPIDAEMIC SUBJECTS
- Author
-
Accinni, R., primary, Dellanoce, C., additional, Bianchi, M., additional, Guinea Montalvo, M., additional, Sedda, V., additional, and Tonini, A., additional
- Published
- 2008
- Full Text
- View/download PDF
20. P407 EFFECTIVENESS OF AN OXIPROLINED MINERAL TRACES SUPPLEMENTATION ON CELLS AND SYSTEMIC REDOX STATE IN HEALTHY SUBJECTS
- Author
-
Accinni, R., primary, Sedda, V., additional, Dellanoce, C., additional, Montalvo, M. Guinea, additional, Tonini, A., additional, Calvelli, L., additional, Ostan, B., additional, Perrone, G., additional, Speciani, A.F., additional, and Vietti, D., additional
- Published
- 2008
- Full Text
- View/download PDF
21. PO20-617 EFFECTS OF FERROSUPER (DRIATEC) SUPPLEMENTATION ON LIPID AND REDOX PARAMETERS IN HEALTHY SUBJECTS
- Author
-
Accinni, R., primary, Sedda, V., additional, Dellanoce, C., additional, Rosina, M., additional, Speciani, A.F., additional, Perrone, G., additional, Ostan, B., additional, and Vietti, D., additional
- Published
- 2007
- Full Text
- View/download PDF
22. PO20-618 EFFECTIVENESS OF A DIETETIC SUPPLEMENTATION IN IMPROVING LIPID PANEL IN DYSLIPIDEMIC SUBJECTS
- Author
-
Sedda, V., primary, Dellanoce, C., additional, Rosina, M., additional, Tonini, A., additional, and Accinni, R., additional
- Published
- 2007
- Full Text
- View/download PDF
23. Tu-P10:420 Time-course of redox-status and inflammatory markers in acute coronary syndromes
- Author
-
Sedda, V., primary, De Chiara, B., additional, Caruso, R., additional, De Maria, R., additional, Dellanoce, C., additional, Caracciolo, M., additional, Mafrici, R., additional, De Biase, A., additional, and Parodi, P., additional
- Published
- 2006
- Full Text
- View/download PDF
24. Urinary neopterin, a new marker of the neuroinflammatory status in amyotrophic lateral sclerosis
- Author
-
Lucio Tremolizzo, Valeria A. Sansone, Andrea Lizio, Jonica Campolo, Christian Lunetta, Claudia Tarlarini, Francesca Gerardi, Lorena Mosca, Cinzia Dellanoce, Susanna Diamanti, Massimo Filippi, Nilo Riva, Lunetta, C, Lizio, A, Gerardi, F, Tarlarini, C, Filippi, M, Riva, N, Tremolizzo, L, Diamanti, S, Dellanoce, C, Mosca, L, Sansone, V, Campolo, J, Lunetta, C., Lizio, A., Gerardi, F., Tarlarini, C., Filippi, M., Riva, N., Tremolizzo, L., Diamanti, S., Dellanoce, C. C., Mosca, L., Sansone, V. A., and Campolo, J.
- Subjects
medicine.medical_specialty ,Neurology ,Prognosi ,Urinary system ,Urine ,Disease ,Gastroenterology ,Neopterin ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Internal medicine ,medicine ,Humans ,030212 general & internal medicine ,Respiratory system ,Amyotrophic lateral sclerosis ,business.industry ,Amyotrophic Lateral Sclerosis ,Biomarker ,Prognosis ,medicine.disease ,Cross-Sectional Studies ,chemistry ,Disease Progression ,Biomarker (medicine) ,Neurology (clinical) ,business ,030217 neurology & neurosurgery ,Biomarkers - Abstract
Objective: To comprehensively assess whether neopterin in urine could be a candidate biomarker for determining the neuroinflammatory status in ALS. Methods: We performed an observational, cross-sectional study in 81 pALS, 68 age- and sex-comparable healthy controls (HC), 14 patients affected by MS and 24 OND patients. ALS patients underwent a neurological evaluation to assess the global functional status evaluated by Amyotrophic Lateral Sclerosis Functional Rating Scale—Revised (ALSFRS-R) and the disease progression rate. Urinary neopterin concentrations were determined by high-performance liquid chromatography method and were recorded at the time of first examination to assess their effect on disease severity and survival. Results: Urinary neopterin was significantly higher in pALS (263.90 [198.71–474.90]) compared to MS (155.28 [131.74–190.38], p =
- Published
- 2020
25. DOAC plasma levels measured by chromogenic anti-Xa assays and HPLC-UV in apixaban- and rivaroxaban-treated patients from the START-Register
- Author
-
Sophie Testa, Daniela Poli, Rossella Marcucci, Claudia Dellanoce, Michela Cini, Benilde Cosmi, Cristina Legnani, Roberto Padrini, Giovanni De Rosa, Vittorio Pengo, Gualtiero Palareti, Cini M., Legnani C., Padrini R., Cosmi B., Dellanoce C., De Rosa G., Marcucci R., Pengo V., Poli D., Testa S., and Palareti G.
- Subjects
Male ,HPLC-UV ,apixaban ,chromogenic anti-Xa assay ,laboratory testing ,rivaroxaban ,medicine.drug_mechanism_of_action ,Pyridones ,Clinical Biochemistry ,Factor Xa Inhibitor ,030204 cardiovascular system & hematology ,Pyridone ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Edoxaban ,medicine ,Humans ,In patient ,Registries ,Chromatography, High Pressure Liquid ,Rivaroxaban ,Chromatography ,Chromogenic ,Biochemistry (medical) ,Antithrombin ,Hematology ,General Medicine ,Plasma levels ,chemistry ,Pyrazole ,Pyrazoles ,Apixaban ,Female ,Drug Monitoring ,030215 immunology ,medicine.drug ,Human ,Factor Xa Inhibitors - Abstract
Introduction: To measure direct factor Xa inhibitor (apixaban, edoxaban, rivaroxaban) concentrations, dedicated chromogenic anti-Xa assays are recommended as suitable methods to provide rapid drug quantification. Moreover, the high-performance liquid chromatography with ultraviolet detection (HPLC-UV) is reported as a reliable quantitative technique. We investigated seven anti-Xa assays and an HPLC-UV method for measurement of apixaban and rivaroxaban levels in patients enrolled in the START-Register. Methods: A total of 127 apixaban and 124 rivaroxaban samples were tested by HPLC-UV and the following anti-Xa assays: Biophen DiXaI and Heparin LRT (Hyphen BioMed), Berichrom and Innovance Heparin (Siemens), STA-Liquid Anti-Xa (Stago Diagnostics), Technochrom anti-Xa (Technoclone), and HemosIL Liquid Anti-Xa (Werfen). Each method was performed in one of the participating laboratories: Bologna, Cremona, Florence, and Padua. Results: Our data confirmed the overestimation of apixaban and rivaroxaban levels by the antithrombin-supplemented anti-Xa method (Berichrom). Performances and reproducibility of the six anti-Xa assays not supplemented with antithrombin and the HPLC-UV method were good, with limits of quantification from 8-39ng/mL (apixaban) and 15-33ng/mL (rivaroxaban). The six chromogenic methods showed good concordances with the quantitative HPLC-UV [bias: −26.9-22.3ng/mL (apixaban), −11.3-18.7ng/mL (rivaroxaban)]. Higher bias and wider range between limits of agreement were observed at higher concentrations [200ng/mL: bias −42.2-36.8ng/mL (apixaban) and −20.1-68.9ng/mL (rivaroxaban)]. Conclusion: Overall, the anti-Xa assays not supplemented with antithrombin and the HPLC-UV method proved to be suitable for apixaban and rivaroxaban quantification.
- Published
- 2020
26. The metabolic syndrome and risk of prostate cancer in Italy.
- Author
-
Pelucchi C, Serraino D, Negri E, Montella M, Dellanoce C, Talamini R, and La Vecchia C
- Subjects
- *
HYPERTENSION epidemiology , *DIABETES , *HYPERCHOLESTEREMIA , *OBESITY , *PROSTATE tumors , *WHITE people , *METABOLIC syndrome , *CASE-control method - Abstract
PURPOSE: To provide information on the role of the metabolic syndrome on prostate cancer risk. METHODS: We examined data from a multicentric Italian case-control study. Cases were 1294 patients with incident, histologically confirmed prostate cancer. Controls were 1451 men hospitalized with acute, non-neoplastic conditions. All subjects were younger than 75 years. The metabolic syndrome was defined according to selected indicators of abdominal obesity, hypercholesterolemia, hypertension, and diabetes. We computed multivariate odds ratios (OR) and 95% confidence intervals (CI) using unconditional logistic regression. RESULTS: Considering separate components of the metabolic syndrome, the ORs were 0.98 (95% CI, 0.72-1.34) for diabetes, 1.14 (95% CI, 0.96-1.36) for hypertension, 1.54 (95% CI, 1.26-1.89) for hypercholesterolemia, and 1.02 (95% CI, 0.86-1.21) for abdominal obesity. The OR of prostate cancer was 1.66 (95% CI, 1.22-2.28) in men with metabolic syndrome compared with those without. We found ORs of 1.02 (95% CI, 0.83-1.26) for men with one component of the metabolic syndrome, 1.12 (95% CI, 0.89-1.42) for two, 1.65 (95% CI, 1.15-2.36) for three, and 3.99 (95% CI, 1.03-15.4) for four compared with no components. CONCLUSIONS: The metabolic syndrome was associated with the risk of prostate cancer in this population. [ABSTRACT FROM AUTHOR]
- Published
- 2011
27. Medium-term effect of sublingual l -glutathione supplementation on flow-mediated dilation in subjects with cardiovascular risk factors
- Author
-
Stefano Bernardi, Silvia Rocchiccioli, Antonella Cecchettini, Annamaria Tonini, Lorena Cozzi, Gualtiero Pelosi, Benedetta De Chiara, Gianpaolo Micheloni, Jonica Campolo, Cristina Giannattasio, Marina Parolini, Cinzia Dellanoce, Oberdan Parodi, Claudio Passino, Campolo, J, Bernardi, S, Cozzi, L, Rocchiccioli, S, Dellanoce, C, Cecchettini, A, Tonini, A, Parolini, M, De Chiara, B, Micheloni, G, Pelosi, G, Passino, C, Giannattasio, C, and Parodi, O
- Subjects
Adult ,medicine.medical_specialty ,Cardiovascular ,Endothelial dysfunction ,Glutathione ,Oxidative stress ,Endocrinology, Diabetes and Metabolism ,Nutrition and Dietetics ,Population ,Administration, Sublingual ,030204 cardiovascular system & hematology ,Placebo ,medicine.disease_cause ,Gastroenterology ,03 medical and health sciences ,Endocrinology ,Vascular Stiffness ,0302 clinical medicine ,Double-Blind Method ,Risk Factors ,Internal medicine ,Humans ,Medicine ,education ,Reactive hyperemia ,education.field_of_study ,Cross-Over Studies ,medicine.diagnostic_test ,business.industry ,MED/11 - MALATTIE DELL'APPARATO CARDIOVASCOLARE ,Middle Aged ,medicine.disease ,Crossover study ,Diabetes and Metabolism ,Cardiovascular Diseases ,Dietary Supplements ,Immunology ,Arterial stiffness ,Endothelium, Vascular ,business ,Lipid profile ,Biomarkers ,030217 neurology & neurosurgery ,Dilatation, Pathologic - Abstract
Objective Supplementation of glutathione (GSH) may be a positive strategy to improve the endogenous antioxidant defense required to counteract many acute and chronic diseases. However, the efficacy of GSH treatment seems to be closely related to type of administration, degree of absorption, and increase of its concentrations. The aim of this study was to test a new sublingual formulation of L-GSH, which enters directly the systemic circulation, to assess its efficacy on circulating biochemical markers of hepatic metabolism, lipid profile, and oxidative stress and on peripheral vascular function compared with placebo in patients with cardiovascular risk factors (CVRF). Methods We enrolled 16 healthy men with CVRF in a double-blinded, randomized placebo-controlled crossover study. At each visit, blood samples were collected for biochemistry analyses and peripheral endothelial function (reactive hyperemia index [RHI]) and stiffness were measured by Endo-PAT2000. Results In the overall population, a decrease in total and low-density lipoprotein cholesterol was highlighted after L-GSH supplementation compared with placebo (P = 0.023 and P = 0.04, respectively). On the contrary, no difference was observed in RHI and oxidative stress markers between L-GSH and placebo in the study population. However, seven participants with baseline abnormal RHI (≤1.67) compared with those with normal RHI showed a significant reduction of arterial stiffness after L-GSH administration, (P = 0.007 and P = 0.037, respectively). Conclusions Supplementation of L-GSH compared with placebo influences the lipid profile of patients with CVRF. Sublingual L-GSH may represent a valid prevention of vascular damage in patients with CVRF and endothelial dysfunction.
- Published
- 2017
28. Drug levels and bleeding complications in atrial fibrillation patients treated with direct oral anticoagulants
- Author
-
Cristina Legnani, Daniela Poli, Rossella Morandini, Oriana Paoletti, Gualtiero Palareti, Emilia Antonucci, Vittorio Pengo, Benilde Cosmi, Armando Tripodi, Roberto Testa, Claudia Dellanoce, Sophie Testa, Testa S., Legnani C., Antonucci E., Paoletti O., Dellanoce C., Cosmi B., Pengo V., Poli D., Morandini R., Testa R., Tripodi A., and Palareti G.
- Subjects
Adult ,Male ,medicine.drug_class ,Pyridones ,Administration, Oral ,Hemorrhage ,direct oral anticoagulant ,030204 cardiovascular system & hematology ,Antithrombins ,Dabigatran ,Drug levels ,03 medical and health sciences ,0302 clinical medicine ,Rivaroxaban ,bleed ,Risk Factors ,level ,Atrial Fibrillation ,medicine ,Humans ,In patient ,Registries ,Blood Coagulation ,Aged ,Aged, 80 and over ,Dose-Response Relationship, Drug ,business.industry ,HAEMOSTASIS ,Anticoagulant ,Atrial fibrillation ,Hematology ,Original Articles ,Middle Aged ,medicine.disease ,peak ,bleeds ,Treatment Outcome ,Italy ,Anesthesia ,Pyrazoles ,Apixaban ,Female ,Original Article ,Drug Monitoring ,business ,medicine.drug ,Blood sampling - Abstract
Essentials Currently, DOACs are given at fixed doses and do not require laboratory monitoring. Direct oral anticoagulant-specific measurements were performed at trough and peak. Patients who developed bleeding events showed higher DOAC plasma levels at peak. This study suggests the need of a more accurate DOAC dose assessment. Background: Direct oral anticoagulants (DOACs) are administered at fixed dose. The aim of the study was to evaluate the relationship between DOAC C-trough or C-peak plasma levels and bleeding complications in patients with non-valvular atrial fibrillation (NVAF). Methods: Five hundred sixty five consecutive naive NVAF patients were enrolled. The DOAC measurements at C-trough and at C-peak (available in 411 patients) were performed at steady state, within the first month of treatment. Major bleeding (MB), clinically relevant non-major bleeding (CRNMB), and minor bleeding (MinB), occurring during 1year of follow-up after blood sampling, were recorded. For each DOAC, interval of C-trough and C-peak levels was subdivided into four equal classes and results were attributed to these classes; the median values of results were also calculated. Results: Two hundred eight patients were on apixaban, 185 on dabigatran, and 172 on rivaroxaban. For 1-[qqqdeletezzz]year follow up for all patients, we observed: 19 MB (3.36%), 6 CRNMB (1.06%), and 47 MinB (8.31%). The prevalence of bleeding patients with anticoagulant levels in the upper classes of C-peak activity (II + III + IV) was higher than that in the lowest class. Normalized results of C-peak levels were higher in patients with bleeding than in those without bleeding. Conclusions: Bleeding complications during DOAC treatment were more frequent among atrial fibrillation (AF) patients with higher C-peak anticoagulant levels. In addition to a previous study that showed an increased risk of thrombotic complications in the patients with low C-trough levels, this study seems to indicate that patients with NVAF on DOACs would need a more accurate definition of their optimal therapeutic window.
- Published
- 2018
29. Low drug levels and thrombotic complications in high-risk atrial fibrillation patients treated with direct oral anticoagulants
- Author
-
Claudia Dellanoce, Rossella Morandini, Armando Tripodi, Sophie Testa, Emilia Antonucci, Vittorio Pengo, Cristina Legnani, Gualtiero Palareti, Roberto Testa, Daniela Poli, Benilde Cosmi, Oriana Paoletti, and Testa S., Paoletti O, Legnani C, Dellanoce C, Antonucci E, Cosmi B, Pengo V, Poli D, Morandini R, Testa R, Tripodi A, Palareti G
- Subjects
Adult ,Male ,medicine.medical_specialty ,Time Factors ,medicine.drug_class ,Pyridones ,Administration, Oral ,030204 cardiovascular system & hematology ,Thrombin time ,Risk Assessment ,Antithrombins ,Dabigatran ,03 medical and health sciences ,0302 clinical medicine ,Rivaroxaban ,Predictive Value of Tests ,Risk Factors ,Internal medicine ,Thromboembolism ,Atrial Fibrillation ,medicine ,Humans ,030212 general & internal medicine ,Registries ,Aged ,Aged, 80 and over ,medicine.diagnostic_test ,business.industry ,Anticoagulant ,Atrial fibrillation ,Hematology ,Middle Aged ,medicine.disease ,Confidence interval ,Treatment Outcome ,Cohort ,Cardiology ,Pyrazoles ,atrial fibrillation, cardiovascular risk, coagulation test, direct oral anticoagulants, thromboembolism, apixaban,dabigatran, rivaroxaban ,Apixaban ,Female ,Blood Coagulation Tests ,Drug Monitoring ,business ,medicine.drug ,Factor Xa Inhibitors ,Preliminary Data - Abstract
Essentials Direct oral anticoagulants (DOACs) do not require laboratory monitoring currently. DOAC specific measurements were performed at trough in patients with atrial fibrillation. Patients who developed thromboembolic events showed lower DOAC plasma levels. This study supports the concept of measuring DOAC levels at steady state. Summary: Background Direct oral anticoagulants (DOACs) are administered at fixed doses without the need for dose adjustment according to laboratory testing. High interindividual variability in drug blood levels has been shown with all DOACs. To evaluate a possible relationship between DOAC C-trough anticoagulant levels and thromboembolic events, 565 consecutive naive patients with atrial fibrillation (AF) were enrolled in this study performed within the START Laboratory Registry. Methods DOAC-specific measurements (diluted thrombin time or anti-activated factor II calibrated for dabigatran; anti-activated FX calibrated for rivaroxaban or apixaban) at C-trough were performed locally at steady state within 15–25 days after the start of treatment. For each DOAC, the interval of C-trough levels, from the limit of quantification to the highest value, was subdivided into four equal classes, and results were attributed to these classes; the median values of results were also calculated. Thromboembolic complications occurring during 1 year of follow-up were recorded. Results Thromboembolic events (1.8%) occurred in 10 patients who had baseline C-trough levels in the lowest class of drug levels. The incidence of thromboembolic events among patients with DOAC C-trough levels in the lowest level class was 2.4%, and that in the remaining groups was 0%. The patients with thrombotic complications also had a higher mean CHA2DS2-VASc score than that of the total patient population: 5.3 (95% confidence interval [CI] 4.3–6.3 versus 3.0 (95% CI 2.9–3.1). Conclusion In this study cohort, thrombotic complications occurred only in DOAC-treated AF patients who had very low C-trough levels, with a relatively high CHA2DS2-VASc score. Larger studies are warranted to confirm these preliminary observations. © 2018 The Authors. Journal of Thrombosis and Haemostasis published by Wiley Periodicals, Inc. on behalf of International Society on Thrombosis and Haemostasis.
- Published
- 2017
30. Comparison of five specific assays for determination of dabigatran plasma concentrations in patients enrolled in the START-Laboratory Register
- Author
-
Rita Paniccia, Armando Tripodi, Vittorio Pengo, C. Legnani, Benilde Cosmi, Claudia Dellanoce, Rossella Marcucci, Sophie Testa, Daniela Poli, Oriana Paoletti, Michela Cini, Gualtiero Palareti, and Cini M, Legnani C, Cosmi B, Testa S, Dellanoce C, Paoletti O, Marcucci R, Poli D, Paniccia R, Pengo V, Tripodi A, Palareti G.
- Subjects
Quality Control ,medicine.medical_specialty ,Clinical Biochemistry ,Urology ,030204 cardiovascular system & hematology ,chromogenic assay, clotting assay, dabigatran, direct thrombin inhibitor, laboratory testing, Antithrombins, Blood Coagulation Tests, Dabigatran,Humans, Limit of Detection, Quality Control, Reproducibility of Results ,Antithrombins ,Dabigatran ,03 medical and health sciences ,0302 clinical medicine ,Limit of Detection ,medicine ,Humans ,In patient ,Detection limit ,business.industry ,Biochemistry (medical) ,Reproducibility of Results ,Hematology ,General Medicine ,Repeatability ,Plasma levels ,Direct thrombin inhibitor ,Plasma concentration ,Blood Coagulation Tests ,business ,030215 immunology ,medicine.drug ,Discovery and development of direct thrombin inhibitors - Abstract
Introduction: Several specific assays are commercially available to determine dabigatran anticoagulant activity. Aims of this multicenter and multiplatform study were to compare five methods for dabigatran measurement and investigate their performances in the low concentration range. Methods: Dabigatran levels were analyzed in 295 plasma samples from patients enrolled in the START-Laboratory Register by the following methods using dedicated calibrators and controls: STA-ECA II (Diagnostica Stago), standard and low range Hemoclot Thrombin Inhibitors (Hyphen BioMed), Direct Thrombin Inhibitor Assay (Instrumentation Laboratory), Direct Thrombin Inhibitor Assay (Siemens), Technoclot DTI (Technoclone). Results: Methods showed variable agreement with the Hemoclot Thrombin Inhibitors assay used as reference test, with modest under- or overestimations (Bland-Altman bias from −17.3 to 4.0 ng/mL). Limits of detection and quantification varied depending on the assay (4-52 and 7-82 ng/mL, respectively). Between-run precision and accuracy were good for all methods for both quality control levels. Assay's repeatability assessed at very low dabigatran concentrations (from 10 to 60 ng/mL) was also acceptable, variability generally increased at lower drug levels. Conclusion: The five dabigatran-specific assays evaluated in this study provided reliable assessment of dabigatran plasma levels, although showing different performances. © 2018 The Authors. International Journal of Laboratory Hematology Published by John Wiley & Sons Ltd
- Published
- 2017
31. Environmental study and stress-related biomarkers modifications in a crew during analog astronaut mission EMMPOL 6.
- Author
-
Giacon TA, Mrakic-Sposta S, Bosco G, Vezzoli A, Dellanoce C, Campisi M, Narici M, Paganini M, Foing B, Kołodziejczyk A, Martinelli M, and Pavanello S
- Abstract
Purpose: Human presence in space is increasingly frequent, but we must not forget that it is a hostile environment. We aimed to study the characteristics of experimental scenarios, to obtain data on human response to isolation, disruption of circadian rhythm and high levels of psychophysical stress., Methods: In these experiments, we evaluated stress response in five young healthy subjects inside an earth-based moon-settlement-like habitat during a 1-week long analog astronaut mission. Wearable devices were used to monitor daily step count of the subjects, physical activity, heart rate during physical exercise and at rest, and sleep parameters. From saliva and urine samples collected every day at awakening, we studied oxy-inflammation biomarkers and hormones (stress and appetite) were studied too., Results: At the end of the week, all subjects revealed an increase in oxidative stress and cortisol levels but no inflammation biomarkers variations, in conjunction with increasing time/daily exercise. Furthermore, a significant decrease in hours of sleep/day, sleep quality, and REM phase of sleep was recorded and correlated with the increase of reactive oxygen species., Conclusion: Oxidative stress increased in a short period of time and may be attributed to the influence of psychological stress during confinement, as well as increased exercise and decreased amount of sleep. On a long-term basis, this could impact performance., (© 2024. The Author(s).)
- Published
- 2024
- Full Text
- View/download PDF
32. More early bleeds associated with high baseline direct oral anticoagulant levels in atrial fibrillation: the MAS study.
- Author
-
Palareti G, Testa S, Legnani C, Dellanoce C, Cini M, Paoletti O, Ciampa A, Antonucci E, Poli D, Morandini R, Tala M, Chiarugi P, Santoro RC, Iannone AM, De Candia E, Pignatelli P, Faioni EM, Chistolini A, Esteban MDP, Marietta M, Tripodi A, and Tosetto A
- Subjects
- Aged, Aged, 80 and over, Female, Humans, Male, Middle Aged, Administration, Oral, Factor Xa Inhibitors therapeutic use, Factor Xa Inhibitors adverse effects, Anticoagulants therapeutic use, Anticoagulants administration & dosage, Anticoagulants adverse effects, Atrial Fibrillation drug therapy, Atrial Fibrillation complications, Hemorrhage chemically induced, Hemorrhage etiology
- Abstract
Abstract: Treatment with direct oral anticoagulants (DOACs) in patients with atrial fibrillation (AF) is effective and safe. However, bleeding complications still occur. Whether DOAC level measurement may further improve treatment efficacy and safety is still an open issue. In the "Measure and See" study, venous blood was collected 15-30 days after DOAC initiation in patients with AF who were then followed up for 1 year to record the occurrence of major and clinically relevant nonmajor bleeding. DOAC plasma levels were measured in 1 laboratory, and results were kept blind to patients and treating doctors. Trough DOAC levels were assessed in 1657 patients (957 [57.7%] and 700 patients treated with standard and low-dose, respectively). Fifty bleeding events were recorded during 1606 years of follow-up (3.11% pt/yrs). Fifteen bleeding events (4.97% pt/yrs) occurred in patients with C-trough standardized values in the highest activity class (>0.50), whereas 35 events (2.69% pt/yrs) occurred in those with values in the 2 lower classes (≤0.50, P = .0401). Increasing DOAC levels and low-dose DOAC use were associated with increased bleeding risk in the first 3 months of treatment. Overall, 19% of patients receiving low doses had standardized values in the highest class. More bleeding occurred in patients on low (4.3% pt/yrs) vs standard (2.2% pt/yrs; P = .0160) dose DOAC. Early measurement of DOAC levels in patients with AF identified many individuals with high levels despite the low doses use and had more bleeding risk during the first 3 months of treatment. This trial was registered at www.ClinicalTrials.gov as #NCT03803579., (© 2024 by The American Society of Hematology. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), permitting only noncommercial, nonderivative use with attribution. All other rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
33. Consumption of Sylimarin, Pyrroloquinoline Quinone Sodium Salt and Myricetin: Effects on Alcohol Levels and Markers of Oxidative Stress-A Pilot Study.
- Author
-
Bosco G, Vezzoli A, Brizzolari A, Paganini M, Giacon TA, Savini F, Gussoni M, Montorsi M, Dellanoce C, and Mrakic-Sposta S
- Subjects
- Humans, Pilot Projects, Male, Adult, Female, Single-Blind Method, PQQ Cofactor pharmacology, Alcohol Drinking, Antioxidants, Ethanol, Middle Aged, Wine analysis, Oxidative Stress drug effects, Biomarkers blood, Biomarkers urine, Flavonoids pharmacology, Flavonoids administration & dosage
- Abstract
Background: Alcohol abuse is one of the most common causes of mortality worldwide. This study aimed to investigate the efficacy of a treatment in reducing circulating ethanol and oxidative stress biomarkers., Methods: Twenty wine-drinking subjects were investigated in a randomized controlled, single-blind trial (ClinicalTrials.gov. Identifier: NCT06548503; Ethical Committee of the University of Padova (HEC-DSB/12-2023) to evaluate the effect of the intake of a product containing silymarin, pyrroloquinoline quinone sodium salt, and myricetin (referred to as Si.Pi.Mi. for this project) on blood alcohol, ethyl glucuronide (EtG: marker for alcohol consumption) and markers of oxidative stress levels (Reactive Oxygen Species-ROS, Total Antioxidant Capacity-TAC, CoQ10, thiols redox status, 8-isoprostane, NO metabolites, neopterin, and uric acid). The effects of the treatment versus placebo were evaluated acutely and after 1 week of supplementation in blood and/or saliva and urine samples., Results: Si.Pi.Mi intake reduced circulating ethanol after 120 min (-33%). Changes in oxidative stress biomarkers, particularly a TAC (range +9-12%) increase and an 8-isoprostane (marker of lipidic peroxidation) decrease (range -22-27%), were observed too., Conclusion: After the administration of Si.Pi.Mi, the data seemed to suggest a better alcohol metabolism and oxidative balance in response to wine intake. Further verification is requested.
- Published
- 2024
- Full Text
- View/download PDF
34. Thrombotic events associated with low baseline direct oral anticoagulant levels in atrial fibrillation: the MAS study.
- Author
-
Testa S, Palareti G, Legnani C, Dellanoce C, Cini M, Paoletti O, Ciampa A, Antonucci E, Poli D, Morandini R, Tala M, Chiarugi P, Santoro RC, Iannone AM, De Candia E, Pignatelli P, Faioni EM, Chistolini A, Esteban MDP, Marietta M, Tripodi A, and Tosetto A
- Subjects
- Humans, Anticoagulants adverse effects, Prospective Studies, Treatment Outcome, Atrial Fibrillation complications, Atrial Fibrillation drug therapy, Atrial Fibrillation epidemiology, Thrombosis chemically induced
- Abstract
Abstract: Although effective and safe, treatment with direct oral anticoagulants (DOAC) in atrial fibrillation (AF) is still associated with thrombotic complications. Whether the measurement of DOAC levels may improve treatment efficacy is an open issue. We carried out the observational, prospective, multicenter Measure and See (MAS) study. Blood was collected 15 to 30 days after starting DOAC treatment in patients with AF who were followed-up for 1 year. Plasma samples were centralized for DOAC level measurement. Patients' DOAC levels were converted into drug/dosage standardized values to allow a pooled analysis in a time-dependent, competitive-risk model. The measured values were transformed into standardized values (representing the distance of each value from the overall mean) by subtracting the DOAC-specific mean value from the original values and dividing by the standard deviation. Trough and peak DOAC levels were assessed in 1657 and 1303 patients, respectively. In total, 21 thrombotic complications were recorded during 1606 years of follow-up (incidence of 1.31% of patients per year). Of 21 thrombotic events, 17 occurred in patients whose standardized activity levels were below the mean of each DOAC (0); the incidence was the highest (4.82% of patients per year) in patients whose standardized values were in the lowest class (-1.00 or less). Early measurement of DOAC levels in patients with AF allowed us to identify most of the patients who, having low baseline DOAC levels, subsequently developed thrombotic complications. Further studies are warranted to assess whether thrombotic complications may be reduced by measuring baseline DOAC levels and modifying treatment when indicated. This trial was registered at www.ClinicalTrials.gov as #NCT03803579., (© 2024 by The American Society of Hematology. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), permitting only noncommercial, nonderivative use with attribution. All other rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
35. Hyperbaric Oxygen Therapy Counters Oxidative Stress/Inflammation-Driven Symptoms in Long COVID-19 Patients: Preliminary Outcomes.
- Author
-
Mrakic-Sposta S, Vezzoli A, Garetto G, Paganini M, Camporesi E, Giacon TA, Dellanoce C, Agrimi J, and Bosco G
- Abstract
Long COVID-19 patients show systemic inflammation and persistent symptoms such as fatigue and malaise, profoundly affecting their quality of life. Since improving oxygenation can oppose inflammation at multiple tissue levels, we hypothesized that hyperbaric oxygen therapy (HBOT) could arrest inflammation progression and thus relieve symptoms of COVID-19. We evaluated oxy-inflammation biomarkers in long COVID-19 subjects treated with HBOT and monitored with non-invasive methods. Five subjects (two athletes and three patients with other comorbidities) were assigned to receive HBOT: 100% inspired O
2 at 2.4 ATA in a multiplace hyperbaric chamber for 90 min (three athletes: 15 HBOT × 5 days/wk for 3 weeks; two patients affected by Idiopathic Sudden Sensorineural Hearing Loss: 30 HBOT × 5 days/wk for 6 weeks; and one patient with osteomyelitis: 30 HBOT × 5 days/wk for week for 6 weeks and, after a 30-day break, followed by a second cycle of 20 HBOT). Using saliva and/or urine samples, reactive oxygen species (ROS), antioxidant capacity, cytokines, lipids peroxidation, DNA damage, and renal status were assessed at T1_pre (basal level) and at T2_pre (basal level after treatment), and the results showed attenuated ROS production, lipid peroxidation, DNA damage, NO metabolites, and inflammation biomarker levels, especially in the athletes post-treatment. Thus, HBOT may represent an alternative non-invasive method for treating long COVID-19-induced long-lasting manifestations of oxy-inflammation.- Published
- 2023
- Full Text
- View/download PDF
36. Chelation Therapy Associated with Antioxidant Supplementation Can Decrease Oxidative Stress and Inflammation in Multiple Sclerosis: Preliminary Results.
- Author
-
Vezzoli A, Mrakic-Sposta S, Dellanoce C, Montorsi M, Vietti D, and Ferrero ME
- Abstract
An imbalance of oxy-inflammation status has been involved in axonal damage and demyelination in multiple sclerosis (MS). The aim of this study was to investigate the efficacy of an antioxidant treatment (calcium disodium ethylenediaminetetracetic acid-EDTA) chelation therapy associated with a micronutrient complex in MS patients. A total of 20 MS patients and 20 healthy subjects, enrolled as a control group (CTR), were recruited. We measured the plasma ROS production and total antioxidant capacity (TAC) by a direct assessment using Electron Paramagnetic Resonance; activities of the antioxidant system (thiols' redox status and enzymes); and the urinary presence of biomarkers of oxidative stress by immunoenzymatic assays. We also evaluated the levels of inflammation by plasmatic cytokines (TNFα, IL-1β, and IL-6) and assessed the sICAM levels, as well as the nitric oxide (NO) catabolism and transthyretin (TTR) concentration. Comparing CTR and MS, in the latter ROS production, oxidative damage, inflammatory biomarkers, and NO metabolite concentrations results were significantly higher, while TAC was significantly lower. Treatment in MS induced significant ( p < 0.05) down-regulating of pro-inflammatory sICAM1, TNF-α, IL6, as well as biomarkers of lipid peroxidation and DNA damage production. The protective effect exhibited may occur by decreasing ROS production and increasing antioxidant capacity, turning into a more reduced thiols' status.
- Published
- 2023
- Full Text
- View/download PDF
37. Glutamatergic dysfunction, neuroplasticity, and redox status in the peripheral blood of patients with motor conversion disorders (functional movement disorders): a first step towards potential biomarkers discovery.
- Author
-
Demartini B, Nisticò V, Benayoun C, Cigognini AC, Ferrucci R, Vezzoli A, Dellanoce C, Gambini O, Priori A, and Mrakic-Sposta S
- Subjects
- Humans, Brain-Derived Neurotrophic Factor, Glutamic Acid, Neuronal Plasticity, Glutamine, Dopamine, Conversion Disorder
- Abstract
Functional movement disorders (FMD) are characterized by the presence of neurological symptoms that cannot be explained by typical neurological diseases or other medical conditions. First evidence showed that, compared to healthy controls (CTR), FMD patients presented increased levels of glutamate+glutamine in the anterior cingulate cortex/medial prefrontal cortex, and decreased levels of glutamate in the cerebrospinal fluid, suggesting that a glutamatergic dysfunction might play a role in FMD pathophysiology. In this study, 12 FMD patients and 20 CTR were recruited and underwent venous blood sampling and urine collection: levels of glutamate, BDNF, dopamine, oxidative stress, creatinine, neopterin, and uric acid were analyzed. Participants also underwent a psychometric assessment investigating depression, anxiety, and alexithymia. We found that levels of glutamate, BDNF, and dopamine were significantly lower in the blood of FMD patients than CTR. Glutamate and dopamine levels were positively associated with levels of alexithymia. Our findings give further evidence that glutamatergic dysfunction might be involved in the pathophysiology of FMD, possibly representing a biomarker of disease; moreover, since glutamatergic and dopaminergic systems are closely interconnected, our results might have a relevance in terms of treatment options for FMD patients., (© 2023. The Author(s).)
- Published
- 2023
- Full Text
- View/download PDF
38. Seasonal Oxy-Inflammation and Hydration Status in Non-Elite Freeskiing Racer: A Pilot Study by Non-Invasive Analytic Method.
- Author
-
Brizzolari A, Bosco G, Vezzoli A, Dellanoce C, Barassi A, Paganini M, Cialoni D, and Mrakic-Sposta S
- Subjects
- Humans, Reactive Oxygen Species, Pilot Projects, Seasons, Interleukin-6, Inflammation, Antioxidants metabolism, Oxidative Stress physiology
- Abstract
Freeskiing is performed in an extreme environment, with significant physical effort that can induce reactive oxygen species (ROS) generation and dehydration. This study aimed to investigate the evolution of the oxy-inflammation and hydration status during a freeskiing training season with non-invasive methods. Eight trained freeskiers were investigated during a season training: T0 (beginning), T1-T3 (training sessions), and T4 (after the end). Urine and saliva were collected at T0, before (A) and after (B) T1-T3, and at T4. ROS, total antioxidant capacity (TAC), interleukin-6 (IL-6), nitric oxide (NO) derivatives, neopterin, and electrolyte balance changes were investigated. We found significant increases in ROS generation (T1A-B +71%; T2A-B +65%; T3A-B +49%; p < 0.05-0.01) and IL-6 (T2A-B +112%; T3A-B +133%; p < 0.01). We did not observe significant variation of TAC and NOx after training sessions. Furthermore, ROS and IL-6 showed statistically significant differences between T0 and T4 (ROS +48%, IL-6 +86%; p < 0.05). Freeskiing induced an increase in ROS production, which can be contained by antioxidant defense activation, and in IL-6, as a consequence of physical activity and skeletal muscular contraction. We did not find deep changes in electrolytes balance, likely because all freeskiers were well-trained and very experienced.
- Published
- 2023
- Full Text
- View/download PDF
39. Differential effect of direct oral anticoagulants on thrombin generation and fibrinolysis in patients with atrial fibrillation and venous thromboembolism.
- Author
-
Dirienzo L, Vitulli A, Mancazzo F, Ammollo CT, Dellanoce C, Paoletti O, Testa S, and Colucci M
- Subjects
- Humans, Dabigatran pharmacology, Dabigatran therapeutic use, Rivaroxaban pharmacology, Rivaroxaban therapeutic use, Thrombin, Fibrin Clot Lysis Time, Fibrinolysis, Tissue Plasminogen Activator, Anticoagulants pharmacology, Anticoagulants therapeutic use, Administration, Oral, Atrial Fibrillation drug therapy, Venous Thromboembolism drug therapy
- Abstract
Background: Recent reports suggest that direct oral anticoagulants (DOAC) may induce different anticoagulant and profibrinolytic responses. We performed a head-to-head comparison of the changes in thrombin generation (TG) parameters and tissue plasminogen activator (t-PA)-induced clot lysis produced by different DOAC., Material and Methods: We tested 137 plasma samples from patients with non-valvular atrial fibrillation (n=72) and venous thromboembolism (n=65) under treatment with apixaban (n=38), edoxaban (n=29), rivaroxaban (n=39), or dabigatran (n=31). TG was evaluated by a fluorometric assay and fibrinolysis by measuring the lysis time of clots exposed to 40 ng/mL t-PA., Results: Trough-to-peak changes of TG parameters, along with correlation analysis, showed that all DOAC prolonged the lag-time in a concentration-dependent fashion. As for the other parameters, anti-factor Xa drugs markedly reduced the thrombin peak and velocity index but had little (rivaroxaban) or no effect on endogenous thrombin potential (ETP); dabigatran, instead, reduced ETP, weakly decreased thrombin peak and did not influence the velocity index, as also inferred from the changes in TG values after neutralisation of dabigatran with idarucizumab. Concerning the profibrinolytic effect of DOAC, intergroup comparison showed that the clot lysis time of dabigatran samples was significantly shorter than that of the apixaban and rivaroxaban samples, at both C-Trough and C-Peak. Moreover, a significant correlation between trough-to-peak changes in drug level and clot lysis time was only observed in the dabigatran group (rho=0.53). Finally, after DOAC removal by DOAC-stop, only dabigatran samples showed a significant increase in lysis time., Discussion: Our data show that dabigatran inhibits TG in a different way than anti-Xa DOAC; moreover, under our conditions, only dabigatran displayed profibrinolytic activity, most likely because of its distinctive effect on the TG curve.
- Published
- 2022
- Full Text
- View/download PDF
40. Effects of Prolonged Exposure to Hypobaric Hypoxia on Oxidative Stress: Overwintering in Antarctic Concordia Station.
- Author
-
Mrakic-Sposta S, Montorsi M, Porcelli S, Marzorati M, Healey B, Dellanoce C, and Vezzoli A
- Subjects
- Antarctic Regions, Humans, Oxygen pharmacology, Reactive Oxygen Species pharmacology, Hypoxia, Oxidative Stress
- Abstract
Concordia Station is the permanent, research station on the Antarctic Plateau at 3230 m. During the eleventh winter-over campaign (DC11-2015; February 2015 to November 2015) at Antarctic Concordia Station, 13 healthy team members were studied and blood samples were collected at six different time points: baseline measurements (T0), performed at sea level before the departure, and during the campaign at 3, 7, 20, 90, and 300 days after arrival at Concordia Station. Reducing the partial pressure of O
2 as barometric pressure falls, hypobaric hypoxia (HH) triggers several physiological adaptations. Among the others, increased oxidative stress and enhanced generation of reactive oxygen/nitrogen species (ROS/RNS), resulting in severe oxidative damage, were observed, which can share potential physiopathological mechanisms associated with many diseases. This study characterized the extent and time-course changes after acute and chronic HH exposure, elucidating possible fundamental mechanisms of adaptation. ROS, oxidative stress biomarkers, nitric oxide, and proinflammatory cytokines significantly increased (range 24-135%) during acute and chronic hypoxia exposure (peak 20th day) with a decrease in antioxidant capacity (peak 90th day: -52%). Results suggest that the adaptive response of oxidative stress balance to HH requires a relatively long time, more than 300th days, as all the observed variables do not return to the preexposition level. These findings may also be relevant to patients in whom oxygen availability is limited through disease (i.e., chronic heart and lung and/or kidney disease) and/or during long-duration space missions., Competing Interests: The authors declare that they have no competing interests., (Copyright © 2022 Simona Mrakic-Sposta et al.)- Published
- 2022
- Full Text
- View/download PDF
41. Oxidative stress and motion sickness in one crew during competitive offshore sailing.
- Author
-
Giacon TA, Bosco G, Vezzoli A, Dellanoce C, Cialoni D, Paganini M, and Mrakic-Sposta S
- Subjects
- Adult, Humans, Lipid Peroxidation, Male, Middle Aged, Motion Sickness physiopathology, Nitric Oxide metabolism, Surveys and Questionnaires statistics & numerical data, Circadian Rhythm physiology, Motion Sickness epidemiology, Oxidative Stress physiology, Water Sports statistics & numerical data
- Abstract
Competitive Offshore Ocean Sailing is a highly demanding activity in which subjects are exposed to psychophysical stressors for a long time. To better define the physiological adaptations, we investigated the stress response of subjects exposed to 3-days long ocean navigation with disruption of circadian rhythms. 6 male subjects were involved in the study and provided urine and saliva samples before setting sail, during a single day of inshore sailing, during 3-days long ocean navigation, and at the arrival, to measure oxidative stress, cortisol, nitric oxide metabolites (NOx) and metabolic response. Motion Sickness questionnaires were also administered during the navigation. The crew suffered a mean weight loss of 1.58 kg. After the long navigation, a significant increase in ROS production and decrease in total antioxidant capacity and uric acid levels were observed. Lipid peroxidation, NO metabolites, ketones, creatinine, and neopterin levels were also increased. Furthermore, a significant increase in cortisol levels was measured. Finally, we found a correlation between motion sickness questionnaires with the increase of NOx, and no correlation with cortisol levels. Physical and psychological stress response derived from offshore sailing resulted in increased oxidative stress, nitric oxide metabolites, and cortisol levels, unbalanced redox status, transient renal function impairment, and ketosis. A direct correlation between motion sickness symptoms evaluated through questionnaires and NOx levels was also found., (© 2022. The Author(s).)
- Published
- 2022
- Full Text
- View/download PDF
42. Impact of centrifugation on thrombin generation in healthy subjects and in patients treated with direct oral anticoagulants.
- Author
-
Hardy M, Dellanoce C, Douxfils J, Carlo A, Lessire S, Bouvy C, Testa S, and Mullier F
- Subjects
- Administration, Oral, Blood Coagulation Tests methods, Healthy Volunteers, Humans, Anticoagulants administration & dosage, Blood Coagulation drug effects, Blood Coagulation Tests standards, Centrifugation adverse effects, Thrombin biosynthesis
- Abstract
Introduction: Double centrifugation before freezing is recommended before thrombin generation assays (TGA). However, this procedure is not mandatory for routine hemostasis tests, precluding the use of these samples for TGA. The aim of this study is to assess the impact of single and double centrifugation on TGA performed on frozen samples from healthy volunteers (HVs) and patients receiving direct oral anticoagulants (DOACs)., Methods: Forty HVs and 57 patients receiving a DOAC (dabigatran, rivaroxaban, apixaban, or edoxaban) were included in this prospective double-center observational study. Blood was collected into 109 mmol/L citrated tubes and frozen at -70°C before TGA using ST Genesia with STG-DrugScreen reagent. Four pre-analytical conditions were studied: (A) single centrifugation (2000 g, 15 minutes) before freezing; (B) one centrifugation before freezing and another after thawing (2000 g, 15 minutes for both); (C) one centrifugation before freezing(2000 g, 15 minutes) and another after thawing (2000 g, 10 minutes); (D) double centrifugation (2000 g, 15 minutes) before freezing (reference). Centrifugation conditions (A), (B), and (C) were compared with the reference condition (D). Acceptable relative differences were defined at 6%, 8%, and 10% for normalized lag time, endogenous thrombin potential, and peak height, respectively., Results: Centrifugation conditions had a small but acceptable impact on HVs samples, but single centrifugation always resulted in unacceptable reductions in normalized lag times for DOAC samples. A second centrifugation after thawing permitted the recovery of acceptable differences for the three TGA parameters for edoxaban but not for apixaban, rivaroxaban, nor dabigatran., Conclusion: Double centrifugation before freezing should remain the recommended pre-analytical condition before TGA., (© 2021 John Wiley & Sons Ltd.)
- Published
- 2021
- Full Text
- View/download PDF
43. Gender-Specific Behaviour in Obesity Stages I-II: Imbalance of Aminothiol Status and Adipomyokine Profile in Subjects with Different Insulin Resistance Severity.
- Author
-
Campolo J, Corradi E, Parolini M, Di Guglielmo ML, Rizzardi A, Dellanoce C, Tarlarini P, Cattaneo M, Scioscioli E, Trivella MG, and De Maria R
- Subjects
- Aged, Female, Humans, Male, Middle Aged, Sex Characteristics, Biomarkers blood, Insulin Resistance genetics, Obesity epidemiology
- Abstract
The hyperproduction of oxidative stress and inflammatory biomarkers, which is paralleled by decreased levels of antioxidant and anti-inflammatory mediators, is part of cellular mechanisms that contribute to the disruption of metabolic homeostasis in obesity. Whether gender-specific alterations and gender-restricted associations in these biomarkers underlie the increased cardiometabolic risk in men compared to women is unclear. We enrolled 31 women and 29 men, aged ≥50 and ≤70 years and with body mass index ≥ 30 and <40 kg/m
2 . We assessed the concentrations of aminothiols (cysteine, homocysteine, and glutathione), expression of oxidant/antioxidant balance, adipomyokines (leptin, adiponectin, myostatin, and interleukin-6), markers of chronic inflammation, and vitamin D, an index of nutritional state, in plasma and serum samples by using HPLC, ELISA, and chemiluminescent immunoassay methods. We measured insulin resistance (IR) by the homeostasis model assessment (HOMA) index. Despite comparable levels of visceral adiposity, IR, and a similar dietary regimen, men showed, with respect to women, higher oxidant concentrations and lower antioxidant levels, which paralleled IR severity. Myostatin levels correlated with prooxidant aminothiols among men only. Gender-specific alterations in aminothiol status and adipomyokine profile and the gender-restricted association between these biomarkers and metabolic derangement are consistent with an increased cardiometabolic risk in men compared to age-matched women with stage I-II obesity. Strict control of redox and inflammatory status, even addressing gender-specific nutritional targets, may be useful to prevent obesity-related metabolic alterations and comorbidities., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Jonica Campolo et al.)- Published
- 2021
- Full Text
- View/download PDF
44. Oxidative Stress and Inflammation, MicroRNA, and Hemoglobin Variations after Administration of Oxygen at Different Pressures and Concentrations: A Randomized Trial.
- Author
-
Bosco G, Paganini M, Giacon TA, Oppio A, Vezzoli A, Dellanoce C, Moro T, Paoli A, Zanotti F, Zavan B, Balestra C, and Mrakic-Sposta S
- Subjects
- Humans, Inflammation, Oxidative Stress, Oxygen, Hyperbaric Oxygenation, MicroRNAs
- Abstract
Exercise generates reactive oxygen species (ROS), creating a redox imbalance towards oxidation when inadequately intense. Normobaric and hyperbaric oxygen (HBO) breathed while not exercising induces antioxidant enzymes expression, but literature is still poor. Twenty-two athletes were assigned to five groups: controls; 30%, or 50% O
2 ; 100% O2 (HBO) at 1.5 or 2.5 atmosphere absolute (ATA). Twenty treatments were administered on non-training days. Biological samples were collected at T0 (baseline), T1 (end of treatments), and T2 (1 month after) to assess ROS, antioxidant capacity (TAC), lipid peroxidation, redox (amino-thiols) and inflammatory (IL-6, 10, TNF-α) status, renal function (i.e., neopterin), miRNA, and hemoglobin. At T1, O2 mixtures and HBO induced an increase of ROS, lipid peroxidation and decreased TAC, counterbalanced at T2. Furthermore, 50% O2 and HBO treatments determined a reduced state in T2. Neopterin concentration increased at T1 breathing 50% O2 and HBO at 2.5 ATA. The results suggest that 50% O2 treatment determined a reduced state in T2; HBO at 1.5 and 2.5 ATA similarly induced protective mechanisms against ROS, despite the latter could expose the body to higher ROS levels and neopterin concentrations. HBO resulted in increased Hb levels and contributed to immunomodulation by regulating interleukin and miRNA expression.- Published
- 2021
- Full Text
- View/download PDF
45. Correction to: Correlates of olfactory impairment in middle-aged non-diabetic Caucasian subjects with stage I-II obesity.
- Author
-
Campolo J, Corradi E, Rizzardi A, Parolini M, Dellanoce C, Di Guglielmo ML, Tarlarini P, Cattaneo M, Trivella MG, and De Maria R
- Published
- 2021
- Full Text
- View/download PDF
46. Correlates of olfactory impairment in middle-aged non-diabetic Caucasian subjects with stage I-II obesity.
- Author
-
Campolo J, Corradi E, Rizzardi A, Parolini M, Dellanoce C, Di Guglielmo ML, Tarlarini P, Cattaneo M, Trivella MG, and De Maria R
- Subjects
- Aged, Female, Humans, Male, Middle Aged, Obesity complications, Obesity epidemiology, Odorants, Sensory Thresholds, Smell, Olfaction Disorders diagnosis, Olfaction Disorders epidemiology, Olfaction Disorders etiology
- Abstract
Purpose: This study evaluates among middle-aged subjects with obesity the prevalence of olfactory impairment (OI) with respect to normative values and its correlation with body composition, cognition, sleep quality, and inflammation., Methods: In 60 (31 women, 29 men) volunteers with a body mass index ≥ 30 to ≤ 40 kg/m
2 , aged ≥ 50 to ≤ 70 years, we assessed olfaction by the Sniffin' Stick test. We measured anthropometrics, body composition and metabolic profiles and evaluated cognition by the MiniMental State Examination (MMSE) and sleep disturbances by the Insomnia Severity Index (ISI). Patients were classified into two groups according to a total olfactory score (odor Threshold, Discrimination, Identification, TDI) below or above the 25th percentile from age and gender-adjusted normative data., Results: Overall, 25 subjects (42%) had OI (TDI < 25th percentile). The largest differences between subjects with and without OI were observed in discrimination and identification scores, with a large overlap in olfactory threshold. Subjects with an abnormal TDI showed significantly higher fat mass index, ISI scores and urinary neopterin and lower MMSE scores than those without OI. By multivariable logistic regression, MMSE, ISI score and urinary neopterin were significantly associated to OI., Conclusions: Among middle-aged subjects with stage I and II obesity, OI is highly prevalent and is independently associated with poor self-reported sleep quality, lower cognition scores and higher levels of the inflammatory marker neopterin.- Published
- 2021
- Full Text
- View/download PDF
47. Prevalence of asymptomatic deep vein thrombosis in patients hospitalized with SARS-CoV-2 pneumonia: a cross-sectional study.
- Author
-
Giorgi-Pierfranceschi M, Paoletti O, Pan A, De Gennaro F, Nardecchia AL, Morandini R, Dellanoce C, Lombi S, Tala M, Cancelli V, Zambelli S, Bosio G, Romanini L, and Testa S
- Subjects
- Aged, Aged, 80 and over, Asymptomatic Diseases epidemiology, COVID-19, Coronavirus Infections epidemiology, Cross-Sectional Studies, Female, Humans, Italy epidemiology, Male, Middle Aged, Pandemics, Pneumonia epidemiology, Pneumonia, Viral epidemiology, Prevalence, Tomography, X-Ray Computed methods, Ultrasonography methods, Venous Thrombosis epidemiology, Venous Thrombosis physiopathology, Coronavirus Infections complications, Pneumonia etiology, Pneumonia, Viral complications, Venous Thrombosis etiology
- Abstract
The association between coronavirus disease 2019 (COVID-19) pneumonia and venous thrombotic disorders is still unclear. We assessed the association between COVID-19 infection-related pneumonia and proximal deep-vein thrombosis (DVT) in a cohort of patients admitted to our hospital during the European outbreak in the front line of Cremona, Lombardy. In a single-center cross-sectional study, all patients hospitalized for more than 5 days in Internal Medicine Department with confirmed COVID-19 pneumonia received 2-point compressive ultrasound assessment (CUS) of the leg vein system during a single day. Ninety-four percent of patients received enoxaparin as standard pharmacological prophylaxis for venous thromboembolism. The presence of DVT was defined as incompressibility of popliteal or common femoral vein. Out of 121 patients with COVID-19 pneumonia (mean age 71.8, 66.3% males) hospitalized on March 31st, 70 stayed in hospital for over 5 days and 66 of them underwent CUS of deep venous system of the legs. The presence of asymptomatic DVT was found in 9 patients (13.6%). No symptomatic DVT was found. Patients with DVT showed mean age = 75.7 years, mean D-dimer levels = 4.02 ng/ml and all of them received enoxaparin for thromboprophylaxis, except one. Computed tomography pulmonary angiogram confirmed pulmonary embolism in five patients. One every seven patients with COVID-19-related pneumonia, hospitalized for more than 5 days, had asymptomatic proximal DVT and half of them had confirmed PE despite standard pharmacological thromboprophylaxis. This observational study suggests the need of an active surveillance through CUS in patients hospitalized with acute SARS-COV-2 and underline the need of a more intense thromboprophylaxis.
- Published
- 2020
- Full Text
- View/download PDF
48. Antioxidant Activity with Increased Endogenous Levels of Vitamin C, E and A Following Dietary Supplementation with a Combination of Glutathione and Resveratrol Precursors.
- Author
-
Biswas P, Dellanoce C, Vezzoli A, Mrakic-Sposta S, Malnati M, Beretta A, and Accinni R
- Subjects
- Acetylcysteine administration & dosage, Aged, Alanine administration & dosage, Ascorbic Acid blood, Erythrocytes metabolism, Female, Glutamine administration & dosage, Glycine administration & dosage, Humans, Ketoglutaric Acids administration & dosage, Male, Middle Aged, Neopterin urine, Oxidation-Reduction, Sulfhydryl Compounds blood, Vitamin A blood, Vitamin E blood, Antioxidants pharmacology, Dietary Supplements, Glucosides administration & dosage, Glutathione metabolism, Resveratrol metabolism, Stilbenes administration & dosage, Vitamins blood
- Abstract
The effects of two different dietary supplements on the redox status of healthy human participants were evaluated. The first supplement (GluS, Glutathione Synthesis) contains the precursors for the endogenous synthesis of glutathione and the second (GluReS, Glutathione and Resveratrol Synthesis) contains in addition polydatin, a precursor of resveratrol. To assess the influence of GluS and GluReS on the redox status, ten thiol species and three vitamins were measured before (t0) and after 8 weeks (t1) of dietary supplementation. An inflammatory marker, neopterin, was also assessed at the same time points. Both supplements were highly effective in improving the redox status by significantly increasing the reduced-glutathione (GSH) content and other reduced thiol species while significantly decreasing the oxidized species. The positive outcome of the redox status was most significant in the GluRes treatment group which also experienced a significant reduction in neopterin levels. Of note, the endogenous levels of vitamins C, E and A were significantly increased in both treatment groups, with best results in the GluReS group. While both dietary supplements significantly contributed to recognized antioxidant and anti-inflammatory outcomes, the effects of GluReS, the combination of glutathione and resveratrol precursors, were more pronounced. Thus, dietary supplementation with GluReS may represent a valuable strategy for maintaining a competent immune status and a healthy lifespan.
- Published
- 2020
- Full Text
- View/download PDF
49. Change in Oxidative Stress Biomarkers During 30 Days in Saturation Dive: A Pilot Study.
- Author
-
Mrakic-Sposta S, Vezzoli A, D'Alessandro F, Paganini M, Dellanoce C, Cialoni D, and Bosco G
- Subjects
- Biomarkers metabolism, Pilot Projects, Reactive Oxygen Species, Diving, Oxidative Stress physiology
- Abstract
Saturation diving allows divers to reduce the risk of decompression sickness while working at depth for prolonged periods but may increase reactive oxygen species (ROS) production. Such modifications can affect endothelial function by exacerbating oxidative stress. This study investigated the effects of saturation diving on oxidative stress damage. Redox status was evaluated through: ROS production; total antioxidant capacity (TAC); nitric oxide metabolites (NOx); nitrotyrosine (3-NT); and lipid peroxidation (8-iso-PGF2α) assessment. Creatinine and neopterin were analyzed as markers of renal function and damage. Measurements were performed on saliva and urine samples obtained at four time points: pre; deep; post; and 24 h post. Four divers were included in the study. After the saturation dive (post), significant ( p < 0.05) increases in ROS (0.12 ± 0.03 vs. 0.36 ± 0.06 µmol.min
-1 ), TAC (1.88 ± 0.03 vs. 2.01 ± 0.08 mM), NOx (207.0 ± 103.3 vs. 441.8 ± 97.3 µM), 3-NT (43.32 ± 18.03 vs. 18.64 ± 7.45 nM·L-1 ), and 8-iso-PGF2α (249.7 ± 45.1 vs. 371.9 ± 54.9 pg·mg-1 creatinine) were detected. Markers of renal damage were increased as well after the end of the saturation dive (creatinine 0.54 ± 0.22 vs. 2.72 ± 1.12 g-L-1 ; neopterin 73.3 ± 27.9 vs. 174.3 ± 20.53 μmol·mol-1 creatinine). These results could ameliorate commercial or military diving protocols or improve the understanding of symptoms caused by oxygen level elevation.- Published
- 2020
- Full Text
- View/download PDF
50. Influence of Dietary Supplementation for Hyperhomocysteinemia Treatments.
- Author
-
Vezzoli A, Dellanoce C, Caimi TM, Vietti D, Montorsi M, Mrakic-Sposta S, and Accinni R
- Subjects
- Adult, Aged, Cysteine blood, Dipeptides blood, Female, Folic Acid blood, Glutathione blood, Humans, Hyperhomocysteinemia blood, Male, Middle Aged, Proline analogs & derivatives, Treatment Outcome, Dietary Supplements, Folic Acid administration & dosage, Hyperhomocysteinemia therapy, Proline administration & dosage, Tetrahydrofolates administration & dosage
- Abstract
Hyperhomocysteinemia is recognized as risk factor for cardiovascular and age-associated diseases. Folic acid supplementation efficiently lowers plasma homocysteine (Hcy) levels, but high intake may negatively affect health because of unnatural levels of unmetabolized folic acid in the systemic circulation. Oxoproline (Oxo) provides by glutamic acid production an increase of intracellular folic acid trapping. Aim of this study was to compare the efficacy of three supplementation protocols: (1) traditional therapy (5-methyl-tetrahydrofolate: 15 mg/day); (2) 5 mL/day of Oxo with 300 μg folic acid (oxifolic); (3) 5 mL/day of Oxo alone (magnesio+) in a 90 days randomized trial on thirty-two moderate hyperhomocysteinemic (18.6 ± 2.4 μmol.L
-1 ) patients (age 48 ± 14 yrs). Thiols: cysteine (Cys), cysteinylglycine (Cys-Gly) and glutathione levels were assessed too. Every supplementation induced significant ( p range <0.05-0.0001) reductions of Hcy level and Cys concentration after the three protocols adopted. Otherwise glutathione concentration significantly increased after oxifolic ( p < 0.01) and traditional ( p < 0.05) supplementation. The integration of Oxo resulted an interesting alternative to traditional therapy because absence or minimal number of folates in the integrator eliminates any chance of excess that can constitute a long-term risk., Competing Interests: The authors declare no conflicts of interest.- Published
- 2020
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.