80 results on '"Degener AM"'
Search Results
2. Rimozione dei virus nei processi di trattamento delle acque reflue
- Author
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Donia, Dt, Gabrieli, R, Degener, Am, Pietrangeli, A, Bonanni, E, Cecchini, E, Di Gianfilippo, F, Anastasi, P, and Divizia, M
- Subjects
Settore MED/42 - Igiene Generale e Applicata - Published
- 2007
3. Keratoacanthoma centrifugum marginatum. Aetiologic role of human papillomavirus and therapeutic effect of etretinate
- Author
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LO SCHIAVO, Ada, PINTO F, DEGENER AM, BUCCI M, RUOCCO, Vincenzo, LO SCHIAVO, Ada, Pinto, F, Degener, Am, Bucci, M, and Ruocco, Vincenzo
- Abstract
Introduction. Keratoacanthoma centrifugum marginatum is an uncommon keratoacanthoma of unknown etiology. We report a case suggesting a possible etiological role for a papillomavirus. Etretinate was an effective treatment.Case report. A 65-year-old woman had keratoacanthoma centrifugum marginatum of the antero-inferior aspect of the lower third of the right leg for 5 years. Fifteen years earlier, an infection had occurred at the same site after a trauma and was treated by oral antibiotics. Surgical exeresis was difficult due to the wide spread of the lesion. Etretinate given at an initial dose of 1 mg/kg/d for 6 weeks then reduced by half for 2 months led to nearly complete cure.Discussion. Keratoacanthoma centrifugum marginatum, verrucous carcinoma and epidermal carcinoma have some histological characteristics in common, suggesting a possible common etiological agent which could be certain strains of the human papillomavirus (HPV). In our case etretinate provided cure, possibly due to its antitumoral activity and perhaps due to its antiviral activity.The presence of koilocytes suggested HPV infection which was confirmed by PCR. This test does not however provide proof of the etiological role of HPV. We are currently studying the presence of HPV in keratoacanthomas and their possible etiological role.
- Published
- 1996
4. Detection of human BK virus sequences in neoplastic prostate tissues: preliminary results
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Pietropaolo, Valeria Antonietta, Mischitelli, M, Fioriti, Daniela, Anzivino, Elena, DI MONACO, F, Degener, Am, Nardacci, R, Piacentini, M, Chiarini, Fernanda, and DI SILVERIO, Franco
- Published
- 2005
5. Detection of viral DNA in bioptic samples from interstitial cystitis patient
- Author
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Fioriti, Daniela, Mischitelli, M, Anzivino, Elena, Penta, M, Degener, Am, Pierangeli, Alessandra, Gentile, Vincenzo, Chiarini, Fernanda, Buttiglieri, Ar, and Pietropaolo, Valeria Antonietta
- Published
- 2005
6. Search of viral DNA in a group of patients with relapsing-remitting multiple sclerosis
- Author
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Pietropaolo, Valeria Antonietta, Fioriti, Daniela, Anzivino, Elena, Penta, M, Santini, M, Millefiorini, E, DI REZZE, S, and Degener, Am
- Published
- 2005
7. Involvement of cellular transcriptional factor Sp-1 and HIV-1 Tat protein in the onset or development of PML
- Author
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Mischitelli, M, Fioriti, D, Videtta, M, Degener, Am, and Pietropaolo, Valeria Antonietta
- Published
- 2004
8. Viral infections and multiple sclerosis: research on remittent-relapsing MS patients
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Mischitelli, M, Videtta, M, Santini, M, Millefiorini, E, Degener, Am, Pietropaolo, Valeria Antonietta, and Nicosia, Rosa
- Published
- 2004
9. Elevata circolazione di genotipi di HPV ad alto rischio in pazienti ambulatoriali
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Degener, Am, Pierangeli, Alessandra, and Dianzani, F.
- Published
- 2002
10. Viral and bacterial coinfections in cervicitis
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Seganti, L, Pisani, S, Gallinelli, C, Lukic, Ankica, Nobili, F, Vetrano, G, Imperi, M, DEGENER AM, ., and Chiarini, Fernanda
- Published
- 1999
11. HSV2 infection of HeLa cells modulates the transcription of integrated HPV18
- Author
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Degener, Am, Pisani, S, Fioriti, D, Seganti, L, and Chiarini, Fernanda
- Published
- 1999
12. Diagnostica di laboratorio delle uretriti ad eziologia multipla
- Author
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Chiarini, Fernanda, Pisani, S, Gallinelli, C, Seganti, L, Funari, Am, and Degener, Am
- Published
- 1998
13. Ricerca di adenovirus in pazienti affetti da uretrite cronica
- Author
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Pisani, S, Gallinelli, C, Chiarini, Fernanda, and Degener, Am
- Published
- 1998
14. Mixed Infection in urethritis: detection and management
- Author
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Chiarini, Fernanda, Pisani, S, Gallinelli, C, Papi, E, Seganti, L, and Degener, Am
- Published
- 1997
15. Simultaneous detection of HPV and Other sexually transmitted agents in chronic urethritis
- Author
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Chiarini, Fernanda, Pisani, S, Gallinelli, C, Papi, E, Seganti, L, and Degener, Am
- Published
- 1997
16. Effect of HSV-2 Infection on the Expression of HPV 16 Genes in Caski Cells
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Pisani, S., primary, Imperi, M., additional, Seganti, L., additional, Superti, F., additional, Tinari, A., additional, Bucci, M., additional, and Degener, AM., additional
- Published
- 2004
- Full Text
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17. Rearrangement Patterns of JC Virus Noncoding Control Region from Different Biological Samples
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Pietropaolo, V., primary, Videtta, M., additional, Fioriti, D., additional, Mischitelli, M., additional, Arancio, A., additional, Orsi, N., additional, and Degener, AM, additional
- Published
- 2003
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18. Trichomonas vaginalis infection: risk indicators among women attending for routine gynecologic examination.
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Verteramo R, Calzolari E, Degener AM, Masciangelo R, and Patella A
- Published
- 2008
19. Role of Human Papillomavirus in the Pathogenesis of Laryngeal Dysplasia.
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Pagliuca G, Martellucci S, Degener AM, Pierangeli A, Greco A, Fusconi M, De Virgilio A, Gallipoli C, de Vincentiis M, and Gallo A
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- Adult, Case-Control Studies, Cross-Sectional Studies, Female, Humans, Laryngeal Neoplasms pathology, Male, Middle Aged, Papillomaviridae isolation & purification, Retrospective Studies, Laryngeal Neoplasms etiology, Papillomavirus Infections complications, Smoking adverse effects
- Abstract
Objective: A synergistic effect between smoking and alcohol intake is the major cause of premalignant and malignant lesions of the larynx, but the risk factors and pathogenesis of the neoplastic transformation in nonsmokers remain poorly defined. The aim of this retrospective study is to establish the relationship between smoking habits and human papillomavirus (HPV) infection in laryngeal dysplasia., Study Design: Cross-sectional study., Setting: Academic university hospital., Subjects and Methods: HPV DNA was amplified from 30 paraffin-embedded laryngeal dysplasia tissue specimens by the polymerase chain reaction using 2 groups of different consensus primers (MYO9/MY11 and LCRF1-4, E7R1-4). Fifteen samples were taken from smokers and 15 from nonsmokers., Results: The present investigation failed to demonstrate the HPV genome in all samples of laryngeal precancerous lesions, whereas HPV was detected in 4 laryngeal papilloma samples used as control to confirm the reliability of our method on paraffin-embedded samples., Conclusions: Although the small number of cases in our series limits the power of our statistical analysis, the absence of viral genomes in the specimens analyzed in this study suggests the lack of a relationship between HPV infection and laryngeal dysplasia in smokers as well as in nonsmokers., (© American Academy of Otolaryngology—Head and Neck Surgery Foundation 2014.)
- Published
- 2014
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20. An epidemiological survey of Mycoplasma hominis and Ureaplasma urealyticum in gynaecological outpatients, Rome, Italy.
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Verteramo R, Patella A, Calzolari E, Recine N, Marcone V, Osborn J, Chiarini F, and Degener AM
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- Adolescent, Adult, Female, Humans, Middle Aged, Outpatients, Prevalence, Rome epidemiology, Sexual Behavior, Young Adult, Mycoplasma Infections epidemiology, Mycoplasma hominis isolation & purification, Reproductive Tract Infections epidemiology, Ureaplasma Infections epidemiology, Ureaplasma urealyticum isolation & purification
- Abstract
The objective of this study was to assess the prevalence of Ureaplasma urealyticum and Mycoplasma hominis infections and to investigate associations between their presence in the lower female genital tract and lifestyle characteristics. The study was performed on a population of 3115 women, comparing the demographic and behavioural characteristics of 872 women with U. urealyticum infection and 142 women with M. hominis with uninfected women, using univariate and multiple logistic regression analysis. The prevalence of infection with U. urealyticum was 28% and M. hominis was 4.6%. In multivariate logistic regression analysis, intrauterine device, number of sexual partners and age (<35 years) were significantly associated with U. urealyticum while previous induced abortion, condom use and young age at first intercourse (<16 years) were associated with M. hominis infection. U. urealyticum infection presents the same demographic and behavioural characteristics of a sexually transmitted disease. The unprotective role of condom use suggests a non-sexual mode of transmission of M. hominis infection.
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- 2013
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21. Epidemiology of Chlamydia trachomatis endocervical infection in a previously unscreened population in Rome, Italy, 2000 to 2009.
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Marcone V, Recine N, Gallinelli C, Nicosia R, Lichtner M, Degener AM, Chiarini F, Calzolari E, and Vullo V
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- Adolescent, Adult, Age Distribution, Amplified Fragment Length Polymorphism Analysis, Chlamydia Infections microbiology, Chlamydia trachomatis genetics, DNA, Bacterial analysis, DNA, Bacterial genetics, Female, Hospitals, University, Humans, Italy epidemiology, Logistic Models, Mass Screening methods, Middle Aged, Population Surveillance, Prevalence, Risk Factors, Sexual Behavior, Socioeconomic Factors, Surveys and Questionnaires, Uterine Cervicitis epidemiology, Uterine Cervicitis microbiology, Young Adult, Chlamydia Infections diagnosis, Chlamydia Infections epidemiology, Chlamydia trachomatis isolation & purification, Uterine Cervicitis diagnosis
- Abstract
As reliable data on Chlamydia trachomatis infection in Italy are lacking and as there is no Italian screening policy, epidemiological analyses are needed to optimise effective strategies for surveillance of the infection in the country. We collected data from 6,969 sexually active women aged 15 to 55 years who underwent testing for endocervical C. trachomatis infection at the Cervico-Vaginal Pathology Unit in the Department of Gynaecology and Obstetrics of Sapienza University in Rome between 2000 and 2009. The mean prevalence of C. trachomatis endocervical infection during this period was 5.2%. Prevalence over time did not show a linear trend. Univariate analysis demonstrated a significant association of infection with multiple lifetime sexual partners, younger age (<40 years), never having been pregnant, smoking, use of oral contraceptives, and human papillomavirus and Trichomonas vaginalis infections. Multivariate stepwise logistic regression showed that T. vaginalis infection, age under 20 years and more than one lifetime sexual partner remained significantly associated with C. trachomatis infection in the final model. Prevalence of C. trachomatis in this study was high, even among women aged 25–39 years (5.1%): our data would suggest that a C. trachomatis screening policy in Italy is warranted, which could lead to a more extensive testing strategy.
- Published
- 2012
22. Nasal polyposis: from pathogenesis to treatment, an update.
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Casale M, Pappacena M, Potena M, Vesperini E, Ciglia G, Mladina R, Dianzani C, Degener AM, and Salvinelli F
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- Adrenal Cortex Hormones therapeutic use, Cell Degranulation, Combined Modality Therapy, Eosinophilia, Histamine Antagonists therapeutic use, Humans, Hypersensitivity, Immediate physiopathology, Hypersensitivity, Immediate therapy, Infections physiopathology, Infections therapy, Nasal Mucosa surgery, Nasal Obstruction, Nasal Polyps physiopathology, Nasal Polyps therapy, Rhinitis, Sinusitis, Hypersensitivity, Immediate complications, Infections complications, Mast Cells immunology, Nasal Mucosa pathology, Nasal Polyps etiology
- Abstract
Nasal polyps (NP) are common benign degeneration of nasal sinus mucosa with a prevalence around 4% in the adult population. The causes are still uncertain but there is a strong association with allergy, infection, asthma and aspirin sensitivity. Histologically, the presence of a large quantity of extracellular fluid, mast cell degranulation and eosinophilia has been demonstrated. Typically the patients show nasal obstruction, anosmia and rhinorrhoea. Nasal endoscopic examination and CT imaging allow evaluation of the disease extension. A combined medical and surgical treatment is recommended for symptoms control in preventing symptomatic NP recurrence. We will review the current knowledge in the pathogenesis and treatment of this complex disease entity.
- Published
- 2011
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23. Diagnosis of anal human papillomavirus infection: polymerase chain reaction or cytology?
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Indinnimeo M, d'Ettorre G, Fiore A, Ceccarelli G, Ciardi A, Degener AM, Moschella CM, Izzo L, Izzo S, Antonelli G, D'Angeli I, and Vullo V
- Subjects
- AIDS-Related Opportunistic Infections diagnosis, AIDS-Related Opportunistic Infections epidemiology, AIDS-Related Opportunistic Infections pathology, AIDS-Related Opportunistic Infections virology, Adult, Aged, Anal Canal virology, Anus Diseases epidemiology, Anus Diseases virology, DNA, Viral analysis, Female, Humans, Male, Middle Aged, Papanicolaou Test, Papillomaviridae genetics, Papillomavirus Infections epidemiology, Papillomavirus Infections virology, Risk-Taking, Sexual Behavior, Vaginal Smears, Young Adult, Anal Canal pathology, Anus Diseases diagnosis, Anus Diseases pathology, Cytodiagnosis methods, HIV Infections complications, Papillomaviridae isolation & purification, Papillomavirus Infections diagnosis, Papillomavirus Infections pathology, Polymerase Chain Reaction methods
- Abstract
Objectives: To investigate the relationship between human immunodeficiency virus (HIV)-positive and HIV-negative patients engaging in promiscuous behaviors and anal human papillomavirus (HPV) infection diagnosed by polymerase chain reaction (PCR) and cytology., Methods: Fifty-six HIV-positive patients and 49 HIV-negative patients who engaged in sexually promiscuous behavior were enrolled in the study. We performed cytological exams using the Pap smear and PCR for HPV-DNA detection, with identification of oncogenic strains. The 2001 Bethesda System terminology was used for the cytological exams. We also evaluated the immunologic status of the HIV-infected patients., Results: PCR positivity for HPV-DNA was higher in the group of HIV-positive patients than in the group of HIV-negative patients with a statistically significant difference. In contrast we did not find any statistically significant difference by cytological exam. Oncogenic strains were equally distributed in the two groups., Conclusions: Our results indicate the importance of the cytological exam for anal HPV screening in the population at high risk of sexually transmitted disease and that HPV-DNA PCR can be used only as adjunct test., (Copyright © 2010 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2011
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24. Ulerythema ophryogenes, a rare and often misdiagnosed syndrome: analysis of an idiopathic case.
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Dianzani C, Pizzuti A, Gaspardini F, Bernardini L, Rizzo B, and Degener AM
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- Abnormalities, Multiple diagnosis, Abnormalities, Multiple genetics, Abnormalities, Multiple pathology, Abnormalities, Multiple virology, Abortion, Spontaneous genetics, Base Sequence, Biopsy, Comparative Genomic Hybridization, DNA, Viral analysis, Darier Disease, Eyebrows abnormalities, Eyebrows pathology, Eyebrows virology, Female, Humans, Karyotyping, Keratosis diagnosis, Keratosis genetics, Keratosis pathology, Keratosis virology, Middle Aged, Molecular Sequence Data, Papillomaviridae genetics, Predictive Value of Tests, Skin virology, Stillbirth genetics, Diagnostic Errors prevention & control, Skin pathology
- Abstract
Keratosis pilaris (KP) is a follicular hyperkeratosis disorder which is frequently detected in the adult population (44%), mostly in female adolescents (80%). It is a genetic autodominant dermatosis with variable penetrance, but no specific gene association has been determined, even though association to the presence of chromosome 18p deletion has been reported in some cases. We report the case of a 51-year-old Caucasian woman affected by keratosis pilaris gradually progressing with age and with a story of multiple abortions. Standard karyotype and CGH array analyses did not reveal any genetic abnormality. Virological analyses detected the presence of HPV 36 DNA inside the dorsum biopsy, leading to hypothesize its involvement in the evolution of the lesion. Clinical history and patient examination led the diagnosis of an idiopathic case of Ulerythema ophryogenes. The analysis of more cases could be useful to verify the involvement of cutaneous HPV in the progression of the clinical manifestation of the KP variants.
- Published
- 2011
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25. Interferon-induced gene expression in cervical mucosa during human papillomavirus infection.
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Pierangeli A, Degener AM, Ferreri ML, Riva E, Rizzo B, Turriziani O, Luciani S, Scagnolari C, and Antonelli G
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- Adolescent, Adult, Cervix Uteri virology, Cytokines genetics, DNA, Viral analysis, Endopeptidases genetics, Female, GTP-Binding Proteins genetics, Gene Expression Regulation, Humans, Middle Aged, Mucous Membrane virology, Myxovirus Resistance Proteins, Papillomaviridae isolation & purification, Papillomavirus Infections virology, RNA, Messenger analysis, Ubiquitin Thiolesterase, Ubiquitins genetics, Viral Load, Cervix Uteri immunology, Interferon Type I physiology, Mucous Membrane immunology, Papillomavirus Infections immunology
- Abstract
The aim of this study is to monitor type I interferon (IFN) activation in the cervical mucosa of Human Papillomavirus (HPV)-infected and uninfected women attending a routine gynaecologic clinic. The expression of three IFN-induced genes (MxA coding for human Mixovirus resistance protein A, ISG15 Interferon Stimulated Gene coding for a 15 kDa ubiquitin-like protein and UBP43 coding for the ISG15 isopeptidase) was determined as the mRNA copy number in cervical cells, normalized to the mRNA ones of the beta-glucuronidase gene. Type-specific HPV-DNA load was concurrently determined in the HPV-positive samples. Out of 127 samples tested, 54 were sufficient for both DNA and RNA extraction. The type-specific HPV-DNA copy numbers in the 34 HPV-positive samples varied widely. No significant association was found between copy numbers of MxA, ISG15, UBP43 and HPV status or viral load. However, despite a marked inter-individual variability, ISG15 expression was significantly higher when low-risk HPV infections were compared with HPV-negative samples, while high-risk HPV infections had very low ISG15 levels. The lack of ISG15 activation in high-risk HPV-infected cervical cells could be due to the lack of p53-mediated induction or to HPV-directed specific inhibition of type I IFN pathways. This study approach might be of value in clarifying the role of type I IFN activation in determining the clearance or persistence of HPV infections.
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- 2011
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26. Giant scrotal elephantiasis: an idiopathic case.
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Dianzani C, Gaspardini F, Persichetti P, Brunetti B, Pizzuti A, Margiotti K, and Degener AM
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- Adult, Elephantiasis diagnosis, Elephantiasis pathology, Humans, Male, Vascular Endothelial Growth Factor Receptor-3 genetics, Elephantiasis surgery, Scrotum pathology
- Abstract
Scrotal elephantiasis is very rare disease in industrialized countries, where it is mainly due to surgery, irradiation or malignancies. It can be defined as idiopathic only when the possible congenital, infectious and compressive causes are excluded. We report a case of massive scrotal lymphoedema in an adult Caucasian patient, in Italy. He presented an extremely voluminous scrotal mass measuring 50 x 47 x 13 cm (weight 18 kg), which extended below his knees, invalidating all his daily activities. The patient was hospitalized in order to undergo to surgical treatment. Although genetic causes were searched and the possible role of infectious agents and compressive factors was evaluated, no etiology was ascertained. Histopathologic examination showed non-specific chronic inflammation, confirming the diagnosis of idiopathic elephantiasis. One year after surgical treatment, the patient is healthy without recurrence signs.
- Published
- 2010
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27. Detection of human papillomavirus and adenovirus in benign and malignant lesions of the larynx.
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Gallo A, Degener AM, Pagliuca G, Pierangeli A, Bizzoni F, Greco A, and de Vincentiis M
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- Academic Medical Centers, Adult, Aged, Aged, 80 and over, Biopsy, Carcinoma, Squamous Cell pathology, Female, Humans, Hyperplasia virology, Laryngeal Diseases virology, Laryngeal Neoplasms pathology, Male, Middle Aged, Papillomavirus Infections pathology, Polymerase Chain Reaction, Predictive Value of Tests, Prospective Studies, Risk Factors, Rome, Tumor Virus Infections virology, Adenoviridae isolation & purification, Carcinoma, Squamous Cell virology, Laryngeal Neoplasms virology, Papillomaviridae isolation & purification, Papillomavirus Infections complications, Tumor Virus Infections complications
- Abstract
Objective: To investigate the role of human papillomavirus (HPV) and adenovirus (AdV) infections in the oncogenesis of squamous cell carcinomas of the larynx and of laryngeal dysplasia., Study Design: Cross-sectional study with planned data collection., Setting: Department of Otorhinolaryngology G. Ferreri and Department of Experimental Medicine and Pathology-Section of Virology, Sapienza University of Rome., Subjects and Methods: Biopsy samples were taken from 68 patients with benign and malignant lesions of the larynx. All tissue samples were analyzed by means of polymerase chain reaction with two groups of primers for HPV and with a pair of primers for AdV., Results: All cases of carcinomas and dysplasia as well as all control cases were negative for both viruses. Four of the five cases of laryngeal papillomas were positive for only HPV, confirming the role of these viral types in the origin of papillomas., Conclusion: The absence of viral genomes in laryngeal carcinomas as in the other cases studied suggests the existence of other factors that play a more important role than viral infection in the carcinogenesis of these lesions.
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- 2009
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28. Human papilloma virus DNA detection, p53 and Ki67 expression in penile verrucous and squamous cell carcinomas in the same patient.
- Author
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Gentile V, Sciarra A, Parente U, Cardillo MR, Pierangeli A, and Degener AM
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- Adult, Carcinoma, Squamous Cell metabolism, Carcinoma, Verrucous metabolism, Humans, Male, Papillomavirus Infections virology, Penile Neoplasms metabolism, Carcinoma, Squamous Cell virology, Carcinoma, Verrucous virology, DNA, Viral isolation & purification, Ki-67 Antigen metabolism, Papillomaviridae genetics, Papillomaviridae isolation & purification, Penile Neoplasms virology, Tumor Suppressor Protein p53 metabolism
- Published
- 2009
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29. Presence and environmental circulation of enteric viruses in three different wastewater treatment plants.
- Author
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Petrinca AR, Donia D, Pierangeli A, Gabrieli R, Degener AM, Bonanni E, Diaco L, Cecchini G, Anastasi P, and Divizia M
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- Coliphages isolation & purification, Enterobacteriaceae isolation & purification, Enterovirus classification, Enterovirus genetics, Feces microbiology, Feces virology, Genome, Viral, Hepatitis A virus classification, Hepatitis A virus genetics, Phylogeny, RNA Phages isolation & purification, RNA, Viral isolation & purification, Enterovirus isolation & purification, Gastrointestinal Diseases virology, Hepatitis A virus isolation & purification, Waste Disposal, Fluid methods, Water Microbiology
- Abstract
Aims: The aim of the work was to evaluate the circulation of the viruses and to determine a correlation between faecal indicators and viruses., Methods and Results: Raw wastewater and effluent samples were collected from three wastewater treatment plants, during three sampling periods, and analysed, using cultural and molecular methods, to determine bacteria and virus presence. The results show a removal of bacterial indicators, but a limited reduction of the phages. The viral analysis displays the circulation of cultivable enteroviruses and differences in the seasonal-geographical distribution. Hepatitis A virus was found with only two genotypes: IA-IB. Rotavirus was present in 11.11%, 24.14%, 2.78% of the samples in the 1st, 2nd and 3rd sampling periods; Astrovirus in 33.33%, 6.9%, 25%; Adenovirus in 7.41%, 3.45%, 2.78%; Norovirus in 7.41%, 10.34%, 5.56% respectively. Adenovirus was never identified in plants B and C as Rotavirus in plant C., Conclusions: The presence of faecal indicators was not predictive of the enteric virus presence, whereas a different circulation of Enteroviruses was found in the wastewater treatment plants., Significance and Impact of the Study: The study shows the importance and the usefulness of molecular methods to evaluate the virus circulation and the genetic variability of Enteroviruses.
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- 2009
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30. Human Papillomaviruses and genital co-infections in gynaecological outpatients.
- Author
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Verteramo R, Pierangeli A, Mancini E, Calzolari E, Bucci M, Osborn J, Nicosia R, Chiarini F, Antonelli G, and Degener AM
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- Adult, Chlamydia trachomatis isolation & purification, Cross-Sectional Studies, Female, Genotype, Humans, Papillomaviridae genetics, Prevalence, Ureaplasma urealyticum isolation & purification, Vaginosis, Bacterial complications, Young Adult, Cervix Uteri microbiology, Chlamydia Infections complications, Papillomaviridae isolation & purification, Papillomavirus Infections complications, Ureaplasma Infections complications
- Abstract
Background: High grade HPV infections and persistence are the strongest risk factors for cervical cancer. Nevertheless other genital microorganisms may be involved in the progression of HPV associated lesions., Methods: Cervical samples were collected to search for human Papillomavirus (HPV), bacteria and yeast infections in gynaecologic outpatients. HPV typing was carried out by PCR and sequencing on cervical brush specimens. Chlamydia trachomatis was identified by strand displacement amplification (SDA) and the other microorganisms were detected by conventional methods., Results: In this cross-sectional study on 857 enrolled outpatients, statistical analyses revealed a significant association of HPV with C. trachomatis and Ureaplasma urealyticum (at high density) detection, whereas no correlation was found between HPV infection and bacterial vaginosis, Streptococcus agalactiae, yeasts, Trichomonas vaginalis and U. urealyticum. Mycoplasma hominis was isolated only in a few cases both in HPV positive and negative women and no patient was infected with Neisseria gonorrhoeae., Conclusion: Although bacterial vaginosis was not significantly associated with HPV, it was more common among the HPV positive women. A significant association between HPV and C. trachomatis was found and interestingly also with U. urealyticum but only at a high colonization rate. These data suggest that it may be important to screen for the simultaneous presence of different microorganisms which may have synergistic pathological effects.
- Published
- 2009
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31. Intra-anal condyloma: surgical or topical treatment?
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Dianzani C, Pierangeli A, Avola A, Borzomati D, Persichetti P, and Degener AM
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- Administration, Topical, Adult, Anal Canal, Anus Diseases pathology, Anus Diseases virology, Condylomata Acuminata pathology, Condylomata Acuminata virology, Humans, Imiquimod, Male, Ointments, Retreatment, Skin Diseases pathology, Skin Diseases virology, Treatment Outcome, Aminoquinolines administration & dosage, Anus Diseases drug therapy, Anus Diseases surgery, Condylomata Acuminata drug therapy, Condylomata Acuminata surgery, Laser Therapy, Skin Diseases drug therapy, Skin Diseases surgery
- Abstract
Human Papillomavirus infections are the strongest risk factors for genital cancer and are the causative agents of anogenital warts. Although the viral types associated with condylomata usually do not cause carcinoma, in women with a history of these lesions there is an increased risk of intraepithelial neoplasia and cancer. Generally the lesions are not life-threatening, but they provoke significant morbidity, are difficult to treat, and are a source of psychosocial stress. Thus, condylomata represent not only a health problem for the patient but also an economic burden for the society. Considering the individual episodes of care, men experience a longer duration of the lesions and incur greater costs than women. We report a case of a male patient with external and intra-anal condyloma resistant to laser therapy. Initially, surgical intervention appeared required because of florid and intra-anal growth. HPV DNA testing and sequencing revealed the presence of HPV 6. After initial discomfort, the lesions were successfully cleared with topical imiquimod 5 percent cream therapy.
- Published
- 2008
32. Type-specific human papillomavirus-DNA load in anal infection in HIV-positive men.
- Author
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Pierangeli A, Scagnolari C, Degener AM, Bucci M, Ciardi A, Riva E, Indinnimeo M, Mancini G, D'Ettorre G, Vullo V, and Antonelli G
- Subjects
- Adult, Aged, DNA, Viral analysis, Genotype, Homosexuality, Male, Humans, Male, Middle Aged, Papillomaviridae genetics, Papillomaviridae isolation & purification, Prospective Studies, Viral Load, AIDS-Related Opportunistic Infections virology, Anus Diseases virology, Papillomaviridae classification, Papillomavirus Infections virology
- Abstract
Objective: To characterize anal human papillomavirus (HPV) infections in terms of genotype prevalence and type-specific DNA load in HIV-positive men., Design: HIV-positive men attending the colo-proctological clinic of a University Hospital in Rome were recruited prospectively from November 2004 to July 2007. HIV-negative outpatients attending the same clinic over the same period were used as a control group., Methods: Anal brushings were tested for HPV-DNA using polymerase chain reactions and direct sequencing; type-specific HPV-DNA copies were measured in most positive samples. HPV data were correlated with patient HIV status and risk factors., Results: HPV-DNA infection was detected in 81% of HIV-positive men. Almost all homosexual men were HPV-infected. The infection rate in low-risk HPV types was higher than in high-risk types. The spectrum of HPV genotypes was comparable between HIV-positive and HIV-negative men. Numbers of HPV-DNA copies varied greatly between samples but did not differ significantly between HIV-positive and HIV-negative men. In many samples, low-risk (HPV 6, 61, 70, and 74) viral loads were comparable with those of high-risk HPVs., Conclusion: Type-specific HPV-DNA copies at baseline appear to be independent of patient immune status and of HPV genotype. HPV genotype risk and viral load should be further evaluated for their potential predictive role in persistence and progression.
- Published
- 2008
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33. Cutaneous human papillomaviruses as recurrence factor in actinic keratoses.
- Author
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Dianzani C, Pierangeli A, Chiricozzi A, Avola A, and Degener AM
- Subjects
- Adult, Aged, DNA, Viral analysis, Female, Humans, Male, Middle Aged, Recurrence, Keratosis virology, Papillomaviridae isolation & purification, Skin virology
- Abstract
Actinic keratoses (AK) are common, premalignant lesions cause mainly by UV DNA damage. Progression into squamous cell carcinoma may be influenced by other several factors such as chronic chemical exposure or viral infection. A carcinogenic role of Human Papillomaviruses (HPV) in early steps of skin tumour development was recently hypothesized; moreover the presence of HPV DNA seems to be higher in cancer precursor lesions. The aim of this work is to identify the presence of HPV DNA in biopsies from Actinic Keratoses (AK) and from normal skin samples collected from dermatological healthy subjects in Italy, in order to evaluate the severity and the clinical evolution of the HPV positive lesions. The DNA test revealed 37% HPV positivity in AK patients versus 0% in the control group; many different genotypes and variants were identified by direct sequencing of PCR product. The HPV positive AK were usually clinically indistinguishable from the HPV negative. All AK lesions were removed by laser treatment, but AK lesions recurred in all HPV positive patients after a period of 45-60 days whereas the same disappeared in the HPV negative ones. These data permit to hypothesize that the presence of HPV DNA could be an aggravating factor for AK lesion severity and recurrence.
- Published
- 2008
- Full Text
- View/download PDF
34. Markers of human papillomavirus infection and their correlation with cervical dysplasia in human immunodeficiency virus-positive women.
- Author
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Riva E, Serraino D, Pierangeli A, Bambacioni F, Zaniratti S, Minosse C, Selleri M, Bucci M, Scagnolari C, Degener AM, Capobianchi MR, Antonelli G, and Dianzani F
- Subjects
- Adult, DNA, Viral genetics, Female, Genetic Markers genetics, Humans, Middle Aged, Papillomaviridae classification, Polymerase Chain Reaction, Species Specificity, HIV, HIV Infections complications, Neoplasms, Squamous Cell complications, Neoplasms, Squamous Cell virology, Papillomaviridae genetics, Papillomavirus Infections complications, Papillomavirus Infections virology, Uterine Cervical Dysplasia complications, Uterine Cervical Dysplasia virology
- Abstract
Human papillomavirus (HPV) genotypes and HPV DNA load were analysed in cervical smears from 76 human immunodeficiency virus (HIV)-positive and 54 HIV-negative women. The prevalence of genotypes was similar for all women, with the exception of HPV62, which was over-represented in HIV-positive samples. HIV-positive women showed a higher prevalence of multiple genotypes that correlated neither with CD4(+) T-cell counts nor with cervical dysplasia. No significant differences were observed in terms of total or single-type HPV DNA load. The HPV DNA load in both HIV-positive and HIV-negative women was significantly higher in squamous intra-epithelial lesions than in negative Pap smears.
- Published
- 2007
- Full Text
- View/download PDF
35. Lactoferrin inhibits early steps of human BK polyomavirus infection.
- Author
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Longhi G, Pietropaolo V, Mischitelli M, Longhi C, Conte MP, Marchetti M, Tinari A, Valenti P, Degener AM, Seganti L, and Superti F
- Subjects
- Animals, BK Virus physiology, Cell Survival, Chlorocebus aethiops, DNA, Viral analysis, Dose-Response Relationship, Drug, Neutralization Tests, Vero Cells, Virus Attachment drug effects, Virus Replication drug effects, Antiviral Agents pharmacology, BK Virus drug effects, Lactoferrin pharmacology
- Abstract
Lactoferrin, a member of the transferrin family, is a bi-globular iron binding glycoprotein, found in milk, exocrine secretions of mammals, and in secondary granules of polymorphonuclear neutrophiles that plays an important role in the defence against various pathogenic microorganisms. Previous studies in different virus-cell systems showed that lactoferrin is a potent inhibitor of different enveloped and naked virus infection. In this research we studied the effect of lactoferrin on BK polyomavirus, a human naked double-stranded DNA virus responsible for productive, persistent, and latent infections of the urinary tract. Results obtained demonstrate that lactoferrin treatment prevents early steps of BK virus infection in Vero cells, at the level of the adsorption phase, probably through the interaction with capsidic structures, although a lactoferrin-BK virus competition for cell plasma-membrane receptors cannot be ruled out.
- Published
- 2006
- Full Text
- View/download PDF
36. Direct sequencing of HPV DNA detected in gynaecologic outpatients in Rome, Italy.
- Author
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Verteramo R, Pierangeli A, Calzolari E, Patella A, Recine N, Mancini E, Marcone V, Masciangelo R, Bucci M, Antonelli G, and Degener AM
- Subjects
- Adolescent, Adult, DNA, Viral genetics, Female, Human papillomavirus 16 genetics, Human papillomavirus 18 genetics, Human papillomavirus 6 genetics, Humans, Middle Aged, Outpatients, Polymerase Chain Reaction methods, Rome, Sequence Analysis, DNA methods, Alphapapillomavirus genetics, DNA, Viral chemistry, Genital Diseases, Female virology, Papillomavirus Infections virology
- Abstract
In order to assess the frequency of different human papillomavirus (HPV) types in Rome and the association between HPV and behavioural characteristics, we tested cervical scrapes of a population of sexually active women referring to university clinics for routine gynaecologic care. The presence of HPV DNA was revealed by polymerase chain reaction on two genome regions (L1 and E6/E7) followed by sequencing. Thirty different HPV types were identified; HPV 16 was the most prevalent (14.18%), followed by HPV 53 (9.21%), HPV 58 (7.80%), HPV 6 and 66 (both 5.67%) whereas all the other genotypes ranged below 5%. In univariate analysis the characteristics significantly associated with HPV DNA detection were the youngest age (P<0.01), the high number of lifetime partners (P<0.001) and the smoking habit (P<0.01). In multiple logistic regression analyses, the characteristics significantly associated with HPV DNA detection remained the younger age and the higher number of lifetime sexual partners. This study may be interesting in order to evaluate the circulation of HPV genotypes in Italy and to add a contribution to anti-cancer vaccine development.
- Published
- 2006
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37. Detection of human papillomavirus DNA, p53 and ki67 expression in penile carcinomas.
- Author
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Gentile V, Vicini P, Giacomelli L, Cardillo MR, Pierangeli A, and Degener AM
- Subjects
- DNA, Viral analysis, DNA, Viral genetics, Genotype, Humans, Immunohistochemistry, Ki-67 Antigen analysis, Lymph Node Excision, Male, Papillomaviridae genetics, Paraffin Embedding, Penile Neoplasms chemistry, Penile Neoplasms surgery, Reverse Transcriptase Polymerase Chain Reaction, Tumor Suppressor Protein p53 analysis, DNA, Viral biosynthesis, Ki-67 Antigen biosynthesis, Papillomaviridae metabolism, Penile Neoplasms metabolism, Tumor Suppressor Protein p53 biosynthesis
- Abstract
Our study is aimed at evaluating the presence of p53 and Ki67 expression by immunohistochemistry in a series of 11 paraffin-embedded penile carcinomas. We also investigated the presence of Human Papillomavirus (HPV) DNA in these tumours and performed an accurate typing by DNA sequencing on positive samples. Immunohistochemistry (IHC) was performed with the anti-p53 and Ki67 mouse monoclonal antibodies. DNA extracted from small sections of each specimen was submitted to amplification with HPV specific general primers; PCR products of the proper length were purified and sequenced. IHC demonstrated nuclear accumulation of mutated p53 and Ki 67 expression in 10/11 tumour samples (90.9%). The prevalence of HPV DNA was 72.7%; the most prevalent type was HPV16. Sequencing analysis revealed the presence of HPV53 (12.5%), HPV18 (25%) and HPV16 (62.5%). Out of the p53 or Ki67 positive carcinomas the percentage of HPV positives was 80% and 70% respectively. Our results indicate that penile carcinoma is frequently associated to high risk HPV and with diffuse p53 and Ki67 expression.
- Published
- 2006
38. Interstitial cystitis and infectious agents.
- Author
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Fioriti D, Penta M, Mischitelli M, Degener AM, Pierangeli A, Gentile V, Nicosia R, Gallinelli C, Chiarini F, and Pietropaolo V
- Subjects
- Adenoviridae chemistry, Adult, BK Virus chemistry, BK Virus genetics, Chlamydia trachomatis chemistry, Chlamydia trachomatis genetics, DNA, Bacterial chemistry, DNA, Bacterial genetics, DNA, Viral analysis, DNA, Viral genetics, Female, Genotype, Herpesvirus 1, Human chemistry, Herpesvirus 1, Human genetics, Herpesvirus 2, Human chemistry, Herpesvirus 2, Human genetics, Humans, Mycoplasma genitalium chemistry, Mycoplasma genitalium genetics, Papillomaviridae chemistry, Papillomaviridae genetics, Reverse Transcriptase Polymerase Chain Reaction, Cystitis, Interstitial microbiology, Cystitis, Interstitial virology
- Abstract
Interstitial cystitis (IC) is a syndrome consisting of severe refractory bladder symptoms of unknown etiology. The disease tends to affect Caucasian women with a mean age of 40 years, with 25% of patients under the age of 30. Few population based epidemiological studies of IC have been performed. We analyzed a case of interstitial cystitis in a 42-year-old non-smoker woman. In two biopsy samples the presence of viral DNA of human polyomavirus BK (BKV), human herpes virus type 1 and type 2 (HHV- 1 and HHV-2), adenovirus, human papillomavirus (HPV) and bacterial DNA (Chlamydia trachomatis and Mycoplasma genitalium) were evaluated by means of polymerase chain reaction (PCR). Both samples resulted positive only for BKV and HPV DNA. HPV genotyping revealed the presence of HPV-66 that is associated with a high risk of cancer development. Thus the finding of a viral co-infection could support the hypothesis of the multi-factorial origin of this pathology.
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- 2005
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39. Investigation on the role of cell transcriptional factor Sp1 and HIV-1 TAT protein in PML onset or development.
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Mischitelli M, Fioriti D, Videtta M, Degener AM, Antinori A, Cinque P, Giordano A, and Pietropaolo V
- Subjects
- Adult, Aged, Base Sequence, Binding Sites, Consensus Sequence genetics, Disease Progression, HIV Seronegativity, HIV Seropositivity cerebrospinal fluid, HIV Seropositivity complications, HIV Seropositivity metabolism, HIV Seropositivity virology, Humans, JC Virus genetics, JC Virus isolation & purification, Leukoencephalopathy, Progressive Multifocal complications, Leukoencephalopathy, Progressive Multifocal virology, Middle Aged, Molecular Sequence Data, Sequence Alignment, tat Gene Products, Human Immunodeficiency Virus, Gene Products, tat metabolism, Leukoencephalopathy, Progressive Multifocal metabolism, Leukoencephalopathy, Progressive Multifocal pathology, Sp1 Transcription Factor metabolism
- Abstract
JC virus (JCV) causes progressive multifocal leukoencephalopathy (PML), characterized by multiple areas of demyelination and attendant loss of brain function. PML is often associated with immunodepression and it is significantly frequent in AIDS patients. The viral genome is divided into early and late genes, between which lies a non-coding control region (NCCR) that regulates JCV replication and presents a great genetic variability. The NCCR of JCV archetype (CY strain) is divided into six regions: A-F containing binding sites for cell factors involved in viral transcription. Deletions and enhancements of these binding sites characterize JCV variants, which could promote viral gene expression and could be more suitable for the onset or development of PML. Therefore, we evaluated by means of polymerase chain reaction (PCR) the presence of JCV genome in cerebrospinal fluid (CSF) of HIV positive and negative subjects both with PML and after sequencing, we analyzed the viral variants found focusing on Sp1 binding sites (box B and D) and up-TAR sequence (box C). It is known that Sp1 activates JCV early promoter and can contribute in maintaining methylation-free CpG islands in active genes, while up-TAR sequence is important for HIV-1 Tat stimulation of JCV late promoter. Our results showed that in HIV-positive subjects all NCCR structures presented enhancements of up-TAR element, whereas in HIV-negative subjects both Sp1 binding sites were always retained. Therefore, we can support the synergism HIV-1/JCV in CNS and we can hypothesize that both Sp1 binding sites could be important to complete JCV replication cycle in absence of HIV-coinfection., (Copyright 2005 Wiley-Liss, Inc.)
- Published
- 2005
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- View/download PDF
40. The human polyomavirus BK: Potential role in cancer.
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Fioriti D, Videtta M, Mischitelli M, Degener AM, Russo G, Giordano A, and Pietropaolo V
- Subjects
- Animals, Antigens, Viral, Tumor metabolism, Cell Transformation, Neoplastic, Cell Transformation, Viral, Genome, Viral, Humans, Polyomavirus Infections physiopathology, BK Virus genetics, BK Virus immunology, Neoplasms virology, Polyomavirus Infections complications
- Abstract
In human cancer, a role has been suggested for the human polyomavirus BK, primarily associated with tubulointerstitial nephritis and ureteric stenosis in renal transplant recipients, and with hemorrhagic cystitis in bone marrow transplant (BMT) recipients. After the initial infection, primarily unapparent and without clinical signs, the virus disseminates and establishes a persistent infection in the urinary tract and lymphocytes. There is correlative evidence regarding potential role of polyomavirus BK in cancer. In fact, the BK virus (BKV) DNA (complete genome and/or subgenomic fragments containing the early region) is able to transform embryonic fibroblasts and cells cultured from kidney and brain of hamster, mouse, rat, rabbit, and monkey. Nevertheless, transformation of human cells by BKV is inefficient and often abortive. Evidence supporting a possible role for BKV in human cancer has accumulated slowly in recent years, after the advent of polymerase chain reaction (PCR). BKV is known to commonly establish persistent infections in people and to be excreted in the urine by individuals who are asymptomatic, complicating the evaluation of its potential role in development of human cancer. Therefore, there is no certain proof that human polyomavirus BK directly causes the cancer in humans or acts as a cofactor in the pathogenesis of some types of human cancer., ((c) 2005 Wiley-Liss, Inc.)
- Published
- 2005
- Full Text
- View/download PDF
41. Detection of human herpesviruses and polyomaviruses DNA in a group of patients with relapsing-remitting multiple sclerosis.
- Author
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Pietropaolo V, Fioriti D, Mischitelli M, Anzivino E, Santini M, Millefiorini E, Di Rezze S, and Degener AM
- Subjects
- Adolescent, Adult, BK Virus genetics, BK Virus isolation & purification, Female, Herpesviridae Infections complications, Herpesvirus 1, Human genetics, Herpesvirus 1, Human isolation & purification, Herpesvirus 2, Human genetics, Herpesvirus 2, Human isolation & purification, Herpesvirus 8, Human genetics, Humans, JC Virus genetics, JC Virus isolation & purification, Leukocytes, Mononuclear virology, Male, Multiple Sclerosis, Relapsing-Remitting complications, Polymerase Chain Reaction, Polyomavirus genetics, Polyomavirus Infections complications, Simplexvirus genetics, Tumor Virus Infections complications, Urine virology, DNA, Viral isolation & purification, Herpesvirus 8, Human isolation & purification, Multiple Sclerosis, Relapsing-Remitting virology, Polyomavirus isolation & purification, Simplexvirus isolation & purification
- Abstract
Multiple sclerosis (MS) is an inflammatory disease of the central nervous system whose pathological features consist of white matter plaques of primary demyelinization and loss of oligodendrocytes. Various risk factors have been associated with MS susceptibility. We have focused this study on different viruses. In particular in the present study we used PCR to search for the genomic DNA of HHV-1, HHV-2, HHV-8, BKV and JCV in urine and peripheral blood mononuclear cells (PBMC) samples from 44 relapsing-remitting MS (RRMS) patients. No viral DNA was found in any urine sample, whereas 29.5% of RRMS PBMC samples were positive. It is suggestive that Human herpesviruses (HHV-1 and HHV-8) were constantly present in all positive samples, indicating that viral agents could contribute to create the demyelination plaques and cause MS.
- Published
- 2005
42. BKV infection and hemorrhagic cystitis after allogeneic bone marrow transplant.
- Author
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Fioriti D, Degener AM, Mischitelli M, Videtta M, Arancio A, Sica S, Sora F, and Pietropaolo V
- Subjects
- Adenoviridae genetics, Adenoviridae isolation & purification, Adenoviridae Infections urine, Adenoviridae Infections virology, Adult, BK Virus genetics, Base Sequence, Cystitis urine, DNA, Viral urine, Female, Hemorrhage urine, Humans, Locus Control Region genetics, Male, Middle Aged, Molecular Sequence Data, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Polyomavirus Infections urine, Sequence Alignment, Transplantation, Homologous, Urine virology, BK Virus isolation & purification, Bone Marrow Transplantation, Cystitis virology, DNA, Viral analysis, Hemorrhage virology, Polyomavirus Infections virology
- Abstract
Hemorrhagic cystitis (HC) is a well-known complication after allogeneic bone marrow transplant (BMT) and can be related to adenovirus or human polyomavirus BK (BKV) infections. In this study a group of 20 patients after allogeneic BMT has been examined. BMT urine samples were analysed for the presence of Adenovirus and BKV DNAby means of polymerase chain reaction (PCR). 5/20 BMT patients developed HC after BMT. The presence of BKV DNA in urine samples was evident in 3/15 patients without HC and in 5/5 patients with HC. In 2/5 HC-patients the BKV DNA was not found after therapy with Cidofovir and Ribavirin. The search for adenovirus DNA in all samples was negative. The analysis of BKV non-coding control region (NCCR) isolated from urine samples revealed a structure very similar to the archetype in all samples. The RFLP (Restriction Fragment Length Polymorphism assay) showed the presence of BKV subtypes I and IV, with the prevalence of subtype I (4/5). This study supports the hypothesis that HC is mainly related to BKV rather than to adenovirus infection in BMT patients. Moreover, since BKV subtype I was predominant, it is reasonable to hypothesize that a specific BKV subtype could be associated with the development of HC.
- Published
- 2005
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43. Prevalence of human papilloma virus type 5 DNA in lesional and non-lesional skin scales of Italian plaque-type psoriatic patients: association with disease severity.
- Author
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Prignano G, Ferraro C, Mussi A, Stivali F, Trento E, Bordignon V, Crescimbeni E, Salvati G, Degener AM, and Ameglio F
- Subjects
- Adult, DNA, Viral isolation & purification, Female, Humans, Male, Middle Aged, Papillomaviridae genetics, Papillomavirus Infections pathology, Papillomavirus Infections virology, Prevalence, Psoriasis pathology, Severity of Illness Index, DNA, Viral analysis, Papillomaviridae isolation & purification, Psoriasis virology, Skin pathology, Skin virology
- Abstract
Human papilloma virus type 5 (HPV-5) has been associated closely with psoriatic skin in Polish patients, while findings from other countries have indicated a more limited prevalence. The results of the present study, in which a type-specific nested PCR was used, indicated that scales of plaque-type psoriatic skin from 54 Italian patients had a high prevalence (74.1%) of HPV-5 DNA in lesional areas, and a reduced prevalence (33.3%) in non-lesional skin (33.3%), compared to 0% of 20 healthy subjects and 3.6% in the lesional areas of 28 patients with various other dermatological diseases. Individuals negative for HPV-5 DNA had a less severe disease. No correlation was found between the presence of HPV DNA and a patient's age or sex. The data demonstrated a statistically significant association between psoriasis and HPV-5, although results in other geographical areas suggest variable virus spread or ethnic variation in virus colonisation.
- Published
- 2005
- Full Text
- View/download PDF
44. Human papillomavirus-32-positive extragenital Bowenoid papulosis (BP) in a HIV patient with typical genital BP localization.
- Author
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Degener AM, Laino L, Pierangeli A, Accappaticcio G, Innocenzi D, and Pala S
- Subjects
- Adult, Anal Canal, Bowen's Disease virology, DNA Primers, DNA, Viral analysis, Diagnosis, Differential, Female, Humans, Lip, Papillomaviridae classification, Papillomaviridae genetics, Papillomavirus Infections virology, Polymerase Chain Reaction, Skin Neoplasms virology, Tumor Virus Infections virology, Vulva, Bowen's Disease diagnosis, HIV Infections, Papillomaviridae isolation & purification, Papillomavirus Infections diagnosis, Skin Neoplasms diagnosis, Tumor Virus Infections diagnosis
- Abstract
Objectives: Bowenoid papulosis (BP) is characterized by multiple maculopapular lesions of the genitalia; extragenital localization is rarely found. Human papillomavirus (HPV) type 16 has been found in most cases of BP, but the other HPV genotypes associated with BP had been poorly characterized., Goal: We describe an extragenital BP with concomitant genital involvement in an HIV-positive woman., Study: Several HPV gene-specific amplifications and sequencing were performed on DNA extracted from biopsy samples., Results: The presence of HPV-16 DNA in anogenital and HPV-32 DNA in lip lesions was demonstrated in the absence of any other HPV type and with no coinfection. Semiquantitative polymerase chain reaction analysis revealed that HPV-16 and -32 DNA sequences are in the episomal state., Conclusions: This is the first report of BP associated with HPV-32 (the causative agent of a benign hyperplasia) and a rare case of BP associated with 2 HPV types in the same patient. The immune depression could have modified the normal benign progression of the extragenital lesions.
- Published
- 2004
- Full Text
- View/download PDF
45. Identification of human papilloma viruses in male dysplastic genital lesions.
- Author
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Dianzani C, Calvieri S, Pierangeli A, and Degener AM
- Subjects
- Adult, Biopsy, Carcinoma, Verrucous virology, Condylomata Acuminata diagnosis, DNA, Viral analysis, Genital Neoplasms, Male diagnosis, Genotype, Humans, Male, Middle Aged, Papillomaviridae genetics, Penile Neoplasms virology, Polymerase Chain Reaction, Condylomata Acuminata virology, Genital Neoplasms, Male virology, Papillomaviridae classification, Papillomaviridae isolation & purification, Papillomavirus Infections virology
- Abstract
The association of Human Papillomaviruses (HPV) DNA with female genital lesions has been widely documented whereas little has been reported about male genital pathologies. The aim of this work was to investigate the presence of HPV DNA and the genotype involved in male dysplastic genital lesions. All samples were analysed by polymerase chain reaction (PCR) to amplify HPV E1 and L1 genes. The PCR products were subjected to restriction fragment length polymorphism (RFLP) to determine the HPV genotype. We analysed 209 male genital biopsies from different lesions: mostly from acuminate condylomata and from Buschke-Lowenstein tumours, Bowen papulosis, leukoplakia of the glans, scrotal lymphangioma, penile horn and penile/perianal verrucous carcinoma. Our results revealed the constant presence of viral DNA in genital condylomata, mainly associated with low risk HPV; the presence of the same genotypes was also detected in some of the examined rare pathologies.
- Published
- 2004
46. Infectious agents in tissues from spontaneous abortions in the first trimester of pregnancy.
- Author
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Penta M, Lukic A, Conte MP, Chiarini F, Fioriti D, Longhi C, Pietropaolo V, Vetrano G, Villaccio B, Degener AM, and Seganti L
- Subjects
- Abortion, Spontaneous epidemiology, Adult, BK Virus, Female, Humans, Incidence, Mycoplasma Infections diagnosis, Mycoplasma fermentans, Polyomavirus Infections diagnosis, Pregnancy, Pregnancy Trimester, First, Tumor Virus Infections diagnosis, Abortion, Spontaneous microbiology, Abortion, Spontaneous virology, Adenovirus Infections, Human diagnosis, Ureaplasma Infections diagnosis, Ureaplasma urealyticum
- Abstract
Some evidence suggests that intrauterine infection plays a major role in the pathogenesis of early pregnancy loss, but the implication and prevalence of microrganisms in the aetiology of spontaneous abortion during the first trimester of pregnancy has not yet been well established. In this study, we analysed the tissues relative to the product of conception from abortions during the first trimester (51 spontaneous abortions and 56 voluntary pregnancy interruptions) in women attending the Gynecological Sciences Perinatology and Puericulture Department of "Policlinico Umberto I". Specimens were investigated by cultural methods for the presence of yeasts, gram positive, gram negative bacteria, and genital mycoplasma. By molecular diagnostic procedures, DNA sequences of Chlamydia trachomatis, herpes simplex viruses, adenovirus, human papillomaviruses and human polyomaviruses BK and JC were searched. None of these agents could be found in voluntary pregnancy interruption samples, with the exception of 3.6% of specimens positive for adenovirus, whereas spontaneous abortion tissues were positive for at least one microrganism by 31.5%. Data analysis showed the occurrence of both monomicrobial and polymicrobial infections.
- Published
- 2003
47. Detection and sequence analysis of human polyomaviruses DNA from autoptic samples of HIV-1 positive and negative subjects.
- Author
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Pietropaolo V, Fioriti D, Simeone P, Videtta M, Di Taranto C, Arancio A, Orsi N, and Degener AM
- Subjects
- Adult, Aged, BK Virus chemistry, BK Virus isolation & purification, Brain Chemistry genetics, Cervix Uteri chemistry, Cervix Uteri virology, Female, HIV Seropositivity mortality, HIV Seropositivity pathology, HIV Seropositivity virology, HIV-1 chemistry, HIV-1 isolation & purification, Humans, JC Virus chemistry, JC Virus isolation & purification, Kidney chemistry, Kidney virology, Male, Middle Aged, Organ Specificity genetics, Spleen chemistry, Spleen virology, BK Virus genetics, HIV Seronegativity genetics, HIV Seropositivity genetics, HIV-1 genetics, JC Virus genetics, Sequence Analysis, DNA methods
- Abstract
The distribution of DNA of BK and JC human polyomaviruses (BKV and JCV) was investigated in samples from autopsies of different organs in 2 groups of patients: Human Immunodeficiency Virus -1 (HIV) positive and negative. Samples from various organs were analysed by a nested polymerase chain reaction (PCR) for the non-coding control and for the VP1 regions of both viruses. The results obtained showed that BKV DNA was present in both males and females with a higher prevalence in HIV-positive subject samples (spleen: 33%; kidney: 44%; brain: 22%, uterine cervix:100%; prostatic urethra: 50%). In prostatic urethra samples of HIV-positive subjects, the JCV DNA was revealed in a low percentage (33%), while it was not found at all in uterine cervix samples of both groups. The varying presence of BK and JC viral DNA in the different organs seems to reflect the different pathogenetic attitude of these viruses. JCV was mainly present in the brain (55%), confirming its typical neurotropism and its etiological role in neurological disorders found in immunodeficient patients. BKV, on the other hand, was mainly present in the kidney (44%) and in genital organs (uterine cervix: 100%; prostatic urethra: 50%) with the latter finding favouring the hypothesis of a possible sexual transmission of BKV. Furthermore, our results confirm the crucial role of the immune system in the persistence of human polyomaviruses in the host.
- Published
- 2003
- Full Text
- View/download PDF
48. Urothelial bladder carcinoma and viral infections: different association with human polyomaviruses and papillomaviruses.
- Author
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Fioriti D, Pietropaolo V, Dal Forno S, Laurenti C, Chiarini F, and Degener AM
- Subjects
- Adult, Aged, Aged, 80 and over, Female, Humans, Male, Middle Aged, Papillomaviridae genetics, Papillomavirus Infections genetics, Papillomavirus Infections pathology, Polyomavirus genetics, Polyomavirus Infections genetics, Polyomavirus Infections pathology, Tumor Virus Infections genetics, Tumor Virus Infections pathology, Urinary Bladder Neoplasms genetics, Urinary Bladder Neoplasms pathology, Papillomavirus Infections virology, Polyomavirus Infections virology, Tumor Virus Infections virology, Urinary Bladder Neoplasms virology
- Abstract
Bladder cancer is the second most commonly occurring genitourinary cancer in adults. The interaction of different carcinogenic and cocarcinogenic agents are responsible for bladder urothelial carcinoma: alcohol and smoking habits, Schistosoma haematobium infection, exposition to chemicals, analgesic and antineoplastic drugs prolonged use. Recently also viral infections have been associated to this pathology. In this study the correlation between viral infections and bladder carcinoma has been evaluated. A group of 32 patients affected by primary bladder neoplasia has been analysed. A control group of 20 autoptic samples of healthy bladder was analysed. The DNA of the following viruses has been searched by polymerase chain reaction (PCR): Adenovirus, Herpes simplex virus type 1 (HSV-1), Herpes simplex virus type 2 (HSV-2), Human Papillomaviruses (HPV), Polyomaviruses (BKV and JCV). In the examined population the association bladder carcinoma-HPV, found by others, has not been confirmed. The high percentage of human polyomaviruses present in the samples is a statistically significant data (p=0.0087) and allows to presume that BKV and JCV may play a role in the aetiology of bladder tumor. In particular the polyomavirus BK, which is found in significative percentage both in single infection (p=0.0036) and in co-infections with other viral species (p=0.035), may be an important co-factor in the pathogenesis of bladder carcinoma.
- Published
- 2003
- Full Text
- View/download PDF
49. Involvement of herpes simplex type 2 in modulation of gene expression of human papillomavirus type 18.
- Author
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Pisani S, Fioriti D, Conte MP, Chiarini F, Seganti L, and Degener AM
- Abstract
Human papillomaviruses (HPVs) and herpes simplex virus type 2 (HSV-2) can establish latent or persistent infections in the host, and are involved in the aetiology of benign and/or malignant lesions of the urogenital tract. To investigate the putative interaction between these DNA viruses when a double infection occurs, we have studied the effect of HSV-2 infection in HeLa 229 cells containing 10-50 copies of HPV type 18 genomic DNA. Twenty hours post HSV-2 infection, the analysis of mRNA transcripts from E1, E2, E6 early and L1 late HPV18 genes was performed in HeLa cells by a semi-quantitative RT-PCR assay. A modulation of HPV18 E1 and E6 early genes was observed, resulting in a 9-fold and 3-fold increased transcription respectively.
- Published
- 2002
- Full Text
- View/download PDF
50. Abnormal "low grade" transformation zone: current diagnostic gold standard.
- Author
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Lukic A, Signore M, Nobili F, Degener AM, Alò PL, Giovagnoli MR, Vetrano G, and Chiarini F
- Subjects
- Base Sequence, Biopsy, Needle, Chlamydia Infections diagnosis, Chlamydia Infections pathology, Colposcopy, Female, Humans, Immunohistochemistry, Molecular Sequence Data, Papillomavirus Infections diagnosis, Papillomavirus Infections pathology, Polymerase Chain Reaction standards, Sensitivity and Specificity, Uterine Cervical Dysplasia diagnosis, Vaginal Smears standards, Cell Transformation, Neoplastic pathology, Polymerase Chain Reaction methods, Precancerous Conditions microbiology, Precancerous Conditions pathology, Uterine Cervical Dysplasia microbiology, Uterine Cervical Dysplasia pathology
- Abstract
The aim of this work was to examine different methods of investigation in the diagnosis of the abnormal "low grade" transformation zone of the portio. Over a period of one year 41 patients subjected to colposcopic examination underwent exo-endocervical sampling for oncologic evaluation and for detection of viral and bacterial infections (HPV, HSV, adenovirus, mycoplasmas and chlamydia trachomatis), as well as portio biopsy. A 65.8% correlation was found between cytology and the HPV-DNA test results, while histology and the presence of the HPV virus agreed in 51.4% of cases. In those cases in which minimal histological alterations were found (koilocytosis) a high percentage of HPV negativity was found. In discordant negative cytologic tests that were however positive for HPV by PCR, the genotypes identified were always 6 and 11.
- Published
- 2002
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