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With increasing urbanization and rapid industrialization, more and more environmental problems have arisen. Phthalates (PAEs) are the foremost and most widespread plasticizers and are readily emitted from these manufactured products into the environment. PAEs act as endocrine-disrupting chemicals (EDCs) and can have serious impacts on aquatic organisms as well as human health. In this study, the water quality criteria (WQC) of five PAEs (dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP), butyl benzyl phthalate (BBP) and di(2-ethylhexyl) phthalate (DEHP)) for freshwater aquatic organisms were developed using a species sensitivity distribution (SSD) and a toxicity percentage ranking (TPR) approach. The results showed that long-term water quality criteria (LWQC) of PAEs using the SSD method could be 13.7, 11.1, 2.8, 7.8, and 0.53 μg/L, respectively. Criteria continuous concentrations (CCC) of PAEs were derived using the TPR method and determined to be 28.4, 13.1, 1.3, 2.5, and 1.6 μg/L, respectively. The five PAEs are commonly measured in China surface waters at concentrations between ng/L and μg/L. DBP, DEHP, and di-n-octyl phthalate (DnOP) were the most frequently detected PAEs, with occurrence rates ranging from 67% to 100%. The ecological risk assessment results of PAEs showed a decreasing order of risk at the national level, DEHP, DBP, DMP, DEP, DnOP. The results of this study will be of great benefit to China and other countries in revising water quality standards for the conservation of aquatic species.

In the tobacco industry, impurity detection is an important prerequisite for ensuring the quality of tobacco. However, in the actual production process, the complex background environment and the variability of impurity shapes can affect the accuracy of impurity detection by tobacco robots, which leads to a decrease in product quality and an increase in health risks. To address this problem, we propose a new online detection method of tobacco impurities for tobacco robot. Firstly, a BCFormer attention mechanism module is designed to effectively mitigate the interference of irrelevant information in the image and improve the network's ability to identify regions of interest. Secondly, a Dual Feature Aggregation (DFA) module is designed and added to Neck to improve the accuracy of tobacco impurities detection by augmenting the fused feature maps with deep semantic and surface location data. Finally, to address the problem that the traditional loss function cannot accurately reflect the distance between two bounding boxes, this paper proposes an optimized loss function to more accurately assess the quality of the bounding boxes. To evaluate the effectiveness of the algorithm, this paper creates a dataset specifically designed to detect tobacco impurities. Experimental results show that the algorithm performs well in identifying tobacco impurities. Our algorithm improved the mAP value by about 3.01% compared to the traditional YOLOX method. The real-time processing efficiency of the model is as high as 41 frames per second, which makes it ideal for automated inspection of tobacco production lines and effectively solves the problem of tobacco impurity detection.

IntroductionThere is a consensus hidden in the criminal legislation of many countries that the criminal responsibility capacity of juvenile offenders is not significantly different from that of their peers. The purpose of this paper was to test this hypothesis. The research objects of this paper were 187 juvenile offenders in J Province, China, who are under detention measures, and 2,449 students from junior high school, senior high school and university in S Province as comparison objects. We subjected the gathered materials to independent-samples t-tests and one-way analysis of variance (ANOVA).Results(1) The self-control ability (109.30, 123.59) and empathy ability (63.86, 72.45) of juvenile offenders were significantly different from those of ordinary minors, but the difference of dialectical thinking ability was not statistically significant; (2) Except for the influence of mother’s education level and family income on dialectical thinking ability, the other variables had no statistical significance on the three kinds of ability. Therefore, it was suggested that the correction plan and means for juvenile offenders should focus on the improvement of self-control ability and empathy ability.

AbstractSchizothorax gulinensis sp. nov. is a new species of the genus Schizothorax from Sichuan, China (Cypriniformes: Cyprinidae). In this study, we have first reported the complete mitochondrial genome of S. gulinensis with Illumina sequencing. There were 16,587 nucleotide pairs in the mitochondrial genome (mitogenome) of S. gulinensis, including 13 protein-coding genes (PCGs), two ribosomal RNAs (rRNAs), and 22 transfer RNAs (tRNAs), as well as one non-coding control region (CR). The proportion of nucleotides in mitochondrial genome was 29.67% (A), 25.45% (T), 17.84% (G), 27.05% (C), and A + T content was 55.12%. All PCGs have the same start codon of the standard ATG, excepting for that of NADH dehydrogenase subunit 1 (nad1) which was the ATC, NADH dehydrogenase subunit 5 (nad5) which was the ATT and cytochrome c oxidase 1 (cox1) which was the ATC. Phylogenetic analysis results supported that S. gulinensis was closely related to Schizothorax grahami. The complete mitochondrial sequence of S. gulinensis will contribute to mitochondrial genome database and provide useful resources for population genetics and evolution analyses.

Hybrid breeding is an effective approach to generate better varieties and prevent variety degradation. The present study investigated the metabolic changes underlying growth superiority in the novel Gymnocypris hybrid (GH), Gymnocypris przewalskii ♀ (GP) × G. eckloni ♂ (GE). The ranking of survival rate was GH > GE > GP, whereas the ranking of growth rate was GE > GH > GP. A proteomic analysis of G. hybrid and its parents was conducted to elucidate the metabolic changes underlying growth superiority. Identified pathways were primarily associated with amino acid, carbohydrate, energy, lipid, and nucleotide metabolism. These metabolic pathways, which are closely associated with growth, are controlled through regulation of the expression of numerous proteins, including adenosylhomocysteinase, hydroxypyruvate reductase, glutamate-cysteine ligase, L-lactate dehydrogenase, creatine kinase, GDP-L-fucose synthase, pyruvate kinase, fructose-bisphosphate aldolase, carbonic anhydrase, phosphopyruvate hydratase, phosphoglycerate kinase, S-(hydroxymethyl) glutathione dehydrogenase, and AMP deaminase. Real-time PCR assays showed that the level of mRNA expression of differentially expressed genes was positively correlated with growth. Proteins that were differentially expressed in GH exhibited fewer differences from GP and more differences from GE. These data are the first to reveal the molecular mechanism whereby growth is regulated in G. hybrid and its parents at the protein level. The study thus provides important information for genetic breeding and improvement of G. hybrid for aquaculture production.

Candida albicans (Ca), a prominent opportunistic fungal pathogen in humans, has garnered considerable attention due to its infectious properties. Herein, we have identified and characterized CaCDAP1 (Ca orf19.1034), a homolog of ScDAP1 found in Saccharomyces cerevisiae. CaCDAP1 encodes a 183-amino acid protein with a conserved cytochrome b5-like heme-binding domain. The deletion of CaDAP1 renders Ca cells susceptible to caspofungin and terbinafine. CaDAP1 deletion confers resistance to Congo Red and Calcofluor White, and sensitivity to sodium dodecyl sulfate. The deletion of CaDAP1 results in a 50% reduction in chitin content within the cell wall, the downregulation of phosphorylation levels in CaMkc1, and the upregulation of phosphorylation levels in CaCek1. Notably, CaDAP1 deletion results in the abnormal hyphal development of Ca cells and diminishes virulence in a mouse systemic infection model. Thus, CaDAP1 emerges as a critical regulator governing cellular responses to antifungal drugs, the synthesis of cell wall chitin, and virulence in Ca.

Gray mold, caused by Botrytis cinerea, poses significant threats to various crops, while it can be remarkably inhibited by ε-poly-L-lysine (ε-PL). A previous study found that B. cinerea extracts could stimulate the ε-PL biosynthesis of Streptomyces albulus, while it is unclear whether the impact of the B. cinerea signal on ε-PL biosynthesis is direct or indirect. This study evaluated the role of elevated reactive oxygen species (ROS) in efficient ε-PL biosynthesis after B. cinerea induction, and its underlying mechanism was disclosed with a transcriptome analysis. The microbial call from B. cinerea could arouse ROS elevation in cells, which fall in a proper level that positively influenced the ε-PL biosynthesis. A systematic transcriptional analysis revealed that this proper dose of intracellular ROS could induce a global transcriptional promotion on key pathways in ε-PL biosynthesis, including the embden-meyerhof-parnas pathway, the pentose phosphate pathway, the tricarboxylic acid cycle, the diaminopimelic acid pathway, ε-PL accumulation, cell respiration, and energy synthesis, in which sigma factor HrdD and the transcriptional regulators of TcrA, TetR, FurA, and MerR might be involved. In addition, the intracellular ROS elevation also resulted in a global modification of secondary metabolite biosynthesis, highlighting the secondary signaling role of intracellular ROS in ε-PL production. This work disclosed the transcriptional mechanism of efficient ε-PL production that resulted from an intracellular ROS elevation after B. cinerea elicitors’ induction, which was of great significance in industrial ε-PL production as well as the biocontrol of gray mold disease.

Abstract Salinity stress progressively reduces plant growth and productivity, while plant has developed complex signaling pathways to confront salt stress. However, only a few genetic variants have been identified to mediate salt tolerance in the major crop rice, and the molecular mechanism remains poorly understood. Here, we identify ten candidate genes associated with salt-tolerance (ST) traits by performing a genome-wide association analysis in rice landraces. We characterize two ST-related genes, encoding transcriptional factor OsWRKY53 and Mitogen-activated protein Kinase Kinase OsMKK10.2, that mediate root Na+ flux and Na+ homeostasis. We further find that OsWRKY53 acts as a negative modulator regulating expression of OsMKK10.2 in promoting ion homeostasis. Furthermore, OsWRKY53 trans-represses OsHKT1;5 (high-affinity K + transporter 1;5), encoding a sodium transport protein in roots. We show that the OsWRKY53-OsMKK10.2 and OsWRKY53-OsHKT1;5 module coordinate defenses against ionic stress. The results shed light on the regulatory mechanisms underlying plant salt tolerance.

Given the increasing incidence of pancreatic cancer and the low survival rate, the exploration of the complex tumor microenvironment and the development of novel treatment options is urgent. NK cells, known for their cytotoxic abilities and modulation of other immune cells, are vital in recognizing and killing cancer cells. However, hypoxic conditions in the tumor microenvironment have been found to impair NK cell functionality and contribute to tumor immune escape. Therefore, we aimed to uncover the mechanism through which hypoxia mediates the immune escape of pancreatic cancer cells, focusing on the influence of miR-1275/AXIN2 on NK cells. Using a combination of GEO dataset screening, Tumor Immune Estimation Resource 2.0 immunoscore screening, and the Cancer Genome Atlas data, we identified a correlation between miR-1275 and NK cells. The down-regulation of miR-1275 was associated with decreased NK cell activity and survival in patients with pancreatic cancer. Pathway analysis further linked miR-1275 expression with the hypoxic HIF1A pathway. In vitro experiments were conducted using the NK-92 cell, revealing that hypoxia significantly reduced miR-1275 expression and correspondingly decreased the cell-killing ability of NK cells. Upregulation of miR-1275 increased perforin, IFN-γ and TNF-α expression levels and enhanced NK cell cytotoxicity. Additionally, miR-1275 was found to bind to and inhibit AXIN2 expression, which when overexpressed, partially alleviated the promotive effect of upregulated miR-1275 on NK-92 cell killing ability. In conclusion, this research underscores the critical role of the miR-1275/AXIN2 axis in hypoxia-mediated immune escape in pancreatic cancer, thus opening new potential avenues for treatment strategies.

Artificial hybrid breeding can optimize parental traits to cultivate excellent hybrids with enhanced economic value. In this study, we investigated the growth performance and transcriptomes of Gymnocypris przewalskii (♀) and Gymnocypris eckloni (♂) and their F1 hybrid fishes. Hatched individuals of G. przewalskii (GP) and G. eckloni (GE) of the same size and their F1 hybrids (GH) were separately cultured for eight months in three cement tanks (n = 3). The growth indexes were measured, which showed that the growth rate of the groups was GE > GH > GP, while the survival rate was GH > GE > GP. The RNA-Seq data analysis of the muscles from the three Gymnocypris fish strains revealed that gene transcription has a significant impact on F1 hybrid fish and its parents. The differentially expressed genes (DEGs) in GH show less differences with GP, but more with GE. qRT-PCR was used to confirm the expression profiles of the chosen DEGs, and the results showed positive correlations with the RNA-seq data. KEGG enrichment results indicated that the DEGs were related to a variety of molecular functions, such as glycolysis/gluconeogenesis, arachidonic acid formation, citrate cycle, and the MAPK, PI3K-Akt, or mTOR signal pathways. Subsequent analysis indicated that there may be a significant correlation between the differential expression of IGF2 and a difference in the growth of GE and GP.

Toxic effects of abamectin on non-target aquatic organisms have been well documented due to its extensive use in both agricultural and aquacultural areas. However, knowledge of the abamectin induced cytotoxicity in crustacean hepatopancreas is still incomplete. In this study, we investigated the cytotoxic effects of abamectin on hepatopancreas cells of Chinese mitten crab, Eriocheir sinensis by an in vitro assay. The results showed that abamectin inhibited cell viability with elevated reactive oxygen species (ROS) and malondialdehyde (MDA) levels in a dose-dependent manner. Increased olive tail moment (OTM) values and 8-hydroxy-2′-deoxyguanosine (8-OHdG) contents indicate the DNA damage under abamectin exposure. The up-regulation of the typical apoptosis-related protein BCL2-associated X protein (Bax) and the down-regulation of B cell leukemia/lymphoma 2 (Bcl-2) demonstrate apoptosis in hepatopancreas cells. Meanwhile, the activities of both caspase-3 and caspase-9 were increased, indicating caspase-mediated apoptosis. In addition, qRT-PCR results showed the up-regulation of antioxidant genes superoxide dismutase (SOD) and catalase (CAT). The mRNA expression of Cap ‘n′ Collar isoform-C (CncC) and c-Jun NH2-terminal kinases (JNK) was also significantly increased, implying the involvement of the Nrf2/MAPK pathway in the antioxidative response. The alteration of innate immune-associated genes Toll-like receptor (TLR) and myeloid differentiation primary response gene 88 (Myd88) also indicates the influence of abamectin on immune status. In summary, the present study reveals the cytotoxicity of abamectin on hepatopancreas cells of E. sinensis and this in vitro cell culture model could be used for further assessment of pesticide toxicity.

Abstract Objective Thyroid cancer (TC) is one of the fastest‐growing malignant tumors. This study sought to explore the mechanism of immune escape mediated by receptor tyrosine kinase (KIT) in TC. Methods The expression microarray of TC was acquired through the GEO database, and the difference analysis and Kyoto encyclopedia of genes and genomes pathway enrichment analysis were carried out. KIT levels in TC cell lines (K1/SW579/BCPAP) and human normal thyroid cells were detected using reverse transcription quantitative polymerase chain reaction and western blot analysis. TC cells were transfected with overexpressed (oe)‐KIT and CD8+ T cells were cocultured with SW579 cells. Subsequently, cell proliferation, migration, and invasion abilities, CD8+ T cell proliferation, cytokine levels (interferon‐γ [IFN‐γ]/tumor necrosis factor‐α [TNF‐α]) were determined using colony formation assay, Transwell assays, flow cytometry, and enzyme‐linked immunosorbent assay. The phosphorylation of MAPK pathway‐related protein (ERK) was measured by western blot analysis. After transfection with oe‐KIT, cells were treated with anisomycin (an activator of the MAPK pathway), and the protein levels of p‐ERK/ERK and programmed death‐ligand 1 (PD‐L1) were detected. Results Differentially expressed genes (N = 2472) were obtained from the GEO database. KIT was reduced in TC samples and lower in tumor cells than those in normal cells. Overexpression of KIT inhibited immune escape of TC cells. Specifically, the proliferation, migration, and invasion abilities of TC cells were lowered, the proliferation level of CD8+ T cells was elevated, and IFN‐γ and TNF‐α levels were increased. KIT inhibited the activation of the MAPK pathway in TC cells and downregulated PD‐L1. Conclusion KIT suppressed immune escape of TC by blocking the activation of the MAPK pathway and downregulating PD‐L1.

Salt is harmful to crop production. Therefore, it is important to understand the mechanism of salt tolerance in rice. CIPK genes have various functions, including regulating salt tolerance and other types of stress and nitrogen use efficiency. In rice, OsCIPK24 is known to regulate salt tolerance, but other OsCIPKs could also function in salt tolerance. In this study, we identified another OsCIPK—OsCIPK9—that can regulate salt tolerance. Knockout of OsCIPK9 in rice could improve salt tolerance. Through expression analyses, OsCIPK9 was found to be mainly expressed in the roots and less expressed in mature leaves. Meanwhile, OsCIPK9 had the highest expression 6 h after salt treatment. In addition, we proved the interaction between OsCIPK9 and OsSOS3. The RNA-seq data showed that OsCIPK9 strongly responded to salt treatment, and the transporters related to salt tolerance may be downstream genes of OsCIPK9. Finally, haplotype analyses revealed that Hap6 and Hap8 mainly exist in indica, potentially providing a higher salt tolerance. Overall, a negative regulator of salt tolerance, OsCIPK9, which interacted with OsSOS3 similarly to OsCIPK24 and influenced salt-related transporters, was identified, and editing OsCIPK9 potentially could be helpful for breeding salt-tolerant rice.

Rice yield and grain quality are highly sensitive to salinity stress. Salt-tolerant/susceptible rice cultivars respond to salinity differently. To explore the variation in grain yield and quality to moderate/severe salinity stress, five rice cultivars differing in degrees of salt tolerance, including three salt-tolerant rice cultivars (Lianjian 5, Lianjian 6, and Lianjian 7) and two salt-susceptible rice cultivars (Wuyunjing 30 and Lianjing 7) were examined. Grain yield was significantly decreased under salinity stress, while the extent of yield loss was lesser in salt-tolerant rice cultivars due to the relatively higher grain filling ratio and grain weight. The milling quality continued to increase with increasing levels. There were genotypic differences in the responses of appearance quality to mild salinity. The appearance quality was first increased and then decreased with increasing levels of salinity stress in salt-tolerant rice but continued to decrease in salt-susceptible rice. Under severe salinity stress, the protein accumulation was increased and the starch content was decreased; the content of short branched-chain of amylopectin was decreased; the crystallinity and stability of the starch were increased, and the gelatinization temperature was increased. These changes resulted in the deterioration of cooking and eating quality of rice under severe salinity-stressed environments. However, salt-tolerant and salt-susceptible rice cultivars responded differently to moderate salinity stress in cooking and eating quality and in the physicochemical properties of the starch. For salt-tolerant rice cultivars, the chain length of amylopectin was decreased, the degrees of order of the starch structure were decreased, and pasting properties and thermal properties were increased significantly, whereas for salt-susceptible rice cultivars, cooking and eating quality was deteriorated under moderate salinity stress. In conclusion, the selection of salt-tolerant rice cultivars can effectively maintain the rice production at a relatively high level while simultaneously enhancing grain quality in moderate salinity-stressed environments. Our results demonstrate specific salinity responses among the rice genotypes and the planting of salt-tolerant rice under moderate soil salinity is a solution to ensure rice production in China.

In this study, the spatial distribution characteristics of nine alkylphenols (APs) in the Yongding River and Beiyun River were analyzed. The differences in the concentrations and spatial distribution patterns of nine APs were systematically evaluated using principal component analysis (PCA). The relationships between the concentration distribution patterns and the risks associated with nine APs were investigated under various categories of land use conditions in the region. The results demonstrated that the APs were widely present in both rivers, and the pollution risks associated with the APs were more severe in the Yongding River than in the Beiyun River. The results show that the contamination risks associated with 4-NP were the most serious in the two rivers, with detection percentages of 100% and 96.3%, respectively. In the Yongding River, the APs showed a tendency of low concentration levels in the upper reaches and high levels in the middle and lower regions. Meanwhile, the overall concentration levels of the APs in the Beiyun River were relatively high. However, despite the differences between the upper and middle regions of the Yongding River, the distribution pattern of the APs in the Beiyun River was basically stable. The concentration levels and risk quotient of the APs were negatively correlated with the vegetation cover land use type and positively correlated with the cropland and unused land use types within 500 m, 1 km, and 2 km. The purpose of this study was to provide theoretical data support and a basis for AP pollution risk evaluations in the Yongding River and Beiyun River.

Abstract Mulberry leaf shape is an important agronomic trait indicating yield, growth, development, and habitat variation. China was the earliest country in the world to grow mulberry for sericulture, and it is also one of the great contributions of the Chinese nation to human civilization. ATAC‐seq (Assay for Transposase Accessible Chromatin using sequencing) is a recently developed technique for genome‐wide analysis of chromatin accessibility. The samples used for ATAC sequencing in this study were divided into two groups of whole leaves (CK‐1 and CK‐2) and lobed leaves (HL‐1 and HL‐2), with two replicates in each group. The related motif analysis, differential expression motif screening, and functional annotation of mulberry leaf shape differences were performed by raw letter analysis to finally obtain the transcription factors (TFs) that lead to the production of heteromorphic leaves. These transcription factors are common in plants, especially the TCP family, shown to be associated with leaf development and growth in other woody plants and are a potential transcription factor responsible for leaf shape differences in mulberry. Dissecting the regulatory mechanisms of leaf shape of different forms of mulberry leaves by ATAC‐seq is an important way to protect mulberry germplasm resources and improve mulberry yield. It is conducive to cultivating mulberry varieties with high resistance to adversity, promoting the sustainable development of sericulture, and protecting and improving the ecological environment.

Objectives To develop a model of in-hospital mortality using medical record front page (MRFP) data and assess its validity in case-mix standardisation by comparison with a model developed using the complete medical record data.Design A nationally representative retrospective study.Setting Representative hospitals in China, covering 161 hospitals in modelling cohort and 156 hospitals in validation cohort.Participants Representative patients admitted for acute myocardial infarction. 8370 patients in modelling cohort and 9704 patients in validation cohort.Primary outcome measures In-hospital mortality, which was defined explicitly as death that occurred during hospitalisation, and the hospital-level risk standardised mortality rate (RSMR).Results A total of 14 variables were included in the model predicting in-hospital mortality based on MRFP data, with the area under receiver operating characteristic curve of 0.78 among modelling cohort and 0.79 among validation cohort. The median of absolute difference between the hospital RSMR predicted by hierarchical generalised linear models established based on MRFP data and complete medical record data, which was built as ‘reference model’, was 0.08% (10th and 90th percentiles: −1.8% and 1.6%). In the regression model comparing the RSMR between two models, the slope and intercept of the regression equation is 0.90 and 0.007 in modelling cohort, while 0.85 and 0.010 in validation cohort, which indicated that the evaluation capability from two models were very similar.Conclusions The models based on MRFP data showed good discrimination and calibration capability, as well as similar risk prediction effect in comparison with the model based on complete medical record data, which proved that MRFP data could be suitable for risk adjustment in hospital performance measurement.

As the agricultural industry, has been an important symbol of national economic development, agricultural development also affects the economic development. In view of this, this paper elaborates the basic theories of agricultural products distribution, combined with the actual situation of agricultural development in Liaoyang area, a more comprehensive analysis of the status quo of the logistics of agricultural products the distribution area of Liaoyang, focusing on its current development process to explore the problems that the main problems of agricultural product logistics as the foundation and implementation of information system construction, from the construction of logistics and the corresponding perfect distribution system and increase investment in infrastructure and other aspects of the strategy and construction Yee.

Emerging technologies are receiving more and more attention from the business community and academia. At present, the research on the entrepreneurial capabilities of emerging technology enterprises is still insufficient. In particular, how to improve the entrepreneurial capabilities of such enterprises in China, there is still no specific research. Therefore, this topic takes the emerging technology enterprises in Anshan as the starting point to study the mechanism and path of the entrepreneurial ability improvement of emerging technology enterprises and provides practical and feasible countermeasures for Liaoning Province and even related enterprises in China.

This paper introduces the relevant theories of strategic management into the formulation of the company's development strategy and promotes the benign development of the company through the analysis, selection and implementation of the company's development strategy. Through the main problems existing in the company's operation, it puts forward corresponding strategies and provides some strategic thinking for the company's development to adapt to changes in the external environment. It hopes to provide valuable suggestions for Tianxin Media's sustainable and healthy development in the future.

Most traditional endmember extraction algorithms focus on spectral information, which limits the effectiveness of endmembers. This paper develops a spatial potential energy weighted maximum simplex algorithm (SPEW) for hyperspectral endmember extraction, combining the relevance of hyperspectral spatial context with spectral information to effectively extract endmembers. Specifically, for pixels in a uniform spatial area, SPEW assigns a high weight to pixels with higher spatial potential energy. For pixels scattered in a spatial area, the high weights are assigned to the representative pixels with a smaller spectral angle distance. Then, the optimal endmember collection is determined by the simplex with maximum volume in the space of representative pixels. SPEW not only reduces the complexity of searching for the maximum simplex volume but also improves the performance of endmember extraction. In particular, compared with other newly proposed spatial-spectral hyperspectral endmember extraction methods, SPEW can effectively extract the hidden endmembers in a spatial area without adjusting any parameters. Experiments on synthetic and real data show that the SPEW algorithm has also provides better results than the traditional algorithms.

Brand in the current market cannot be ignored in agricultural trade competitiveness. Agricultural brand building for solving agricultural products "difficult to sell, low price," the problem, increase the incomes of agricultural industry practitioners, and promoting regional economic development and improving people's livelihood, help Guizhou Province operators in the agricultural and agro-agricultural market access to the entire community recognition. First of all, this paper reviews the theory of agricultural product brand building, analyzes the significance and Countermeasures of building agricultural product brand, and the specialty, diversity and externality of the brand of agricultural products. Second, the current situation of Guizhou Province agricultural product brand construction and development and problems that the current Guizhou Province agricultural product brand construction mainly has the brand building is not perfect, there is a brand of agricultural products uneven distribution, small scale and low brand value. Third, this paper analyzes the reasons for the lack of agricultural product brand construction and development in Guizhou Province, and points out that the quality of agricultural products is not high, and the brand protection mechanism is imperfect, etc., which delays or even hinders the process of brand building. Finally, this paper puts forward some suggestions for the construction of Guizhou Province agricultural products brand, brand construction of agricultural products according to the model constructed by the combination of Guizhou Province, the development of Industry Association, the role of government policy, play, product quality, and image promotion of regional agricultural products brand building and development in the "colorful Guizhou" tourism etc.

This paper presents part of a study on students’ entrepreneurial intention in doing information-related business when they graduated from their studies. It presents the findings of two objectives of the study: 1) To determine the level of students’ attitude towards behaviour in doing information-related business, 2) To compare students’ attitude towards behaviour in doing information-related business in terms of gender, roles of family background, and students’ family income. Questionnaires were distributed randomly to 175 postgraduate students of Faculty of Information Management, Universiti Teknologi MARA (UiTM), Malaysia. In general, students are quite positive about their attitude towards behaviour to start a business venture. However there were no evidences of differences regarding students’ attitude towards behaviour in doing information-related business in terms of gender, role of family background and family income.

In recent years, with the development of China's economy, the living standards of the people have been continuously improved, and the demand for food quality and freshness has become higher and higher, especially for fresh produce. Based on the analysis of the current situation of the company and the problems in the management of cold chain logistics, this paper establishes a cold-chain logistics management mode that meets the Bacardi companies. Through to the Bacardi implementation of the principle of cold chain logistics management mode, management mode and purpose is analyzed, the main node to Bacardi company provides intelligent pave the way for the development of cold chain logistics system, which guarantees for the implementation of the cold chain logistics management.

Thymoma is one of the thymic epithelial tumors arising from the thymic epithelial cells of a variety of animal species including cat, cattle, dog, goat, horse, and pig. The cancer cells of thymoma are always localized within thymus. Feline thymoma was usually identified in the cranial mediastinum of elder cats. In this report, we present the full diagnostic characteristics of a young cat with a cranial mediastinal mass diagnosed as a cystic type B1 thymoma. A 3-yearold male neutered domestic short-haired cat was referred for further diagnosis due to the shortness of breath and pleural accumulation. One single cystic extra-pericardial mass was seen and the boundary between the mass and the surrounding tissue was clearly demarcated in three-view thoracic radiographs. Ultrasound showed pleural effusion and intra-thoracic mass. Pleural fluid biochemistry showed high level of triglycerides and complete blood count revealed the elevated number of basophils and high level of feline serum amyloid A (fSAA). Pleural effusion sediment cytology showed a large number of round cells, mainly small lymphocytes, as well as a moderate number of neutrophils and a small number of large lymphocytes. The high level of triglycerides in pleural fluid instead of serum and high level of fSAA seem to provide informative clues to the diagnosis of thymoma and are worthy of further investigation. [ABSTRACT FROM AUTHOR]