1. An ancient family of lytic polysaccharide monooxygenases with roles in arthropod development and biomass digestion
- Author
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Sabbadin, Federico, Hemsworth, Glyn R., Ciano, Luisa, Henrissat, Bernard, Dupree, Paul, Tryfona, Theodora, Marques, Rita D. S., Sweeney, Sean T., Besser, Katrin, Elias, Luisa, Pesante, Giovanna, Li, Yi, Dowle, Adam A., Bates, Rachel, Gomez, Leonardo D., Simister, Rachael, Davies, Gideon J., Walton, Paul H., Bruce, Neil C., McQueen-Mason, Simon J., University of York [York, UK], University of Leeds, Architecture et fonction des macromolécules biologiques (AFMB), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), University of Cambridge [UK] (CAM), Hemsworth, Glyn R [0000-0002-8226-1380], Ciano, Luisa [0000-0002-1667-0856], Dupree, Paul [0000-0001-9270-6286], Sweeney, Sean T [0000-0003-2673-9578], Davies, Gideon J [0000-0002-7343-776X], Walton, Paul H [0000-0002-1152-1480], Apollo - University of Cambridge Repository, Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA), UK Biotechnology and Biological Sciences Research Council [BB/L001926/1], Yorkshire Forward, Northern Way Initiative, and EPSRC [EP/K039660/1, EP/M028127/1]
- Subjects
Models, Molecular ,Proteomics ,Insecta ,Science ,Chitin ,Genes, Insect ,Article ,Mixed Function Oxygenases ,Evolution, Molecular ,Polysaccharides ,Animals ,Biomass ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] ,lcsh:Science ,Cellulose ,Arthropods ,Phylogeny ,food and beverages ,Enzymes ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM] ,Biodegradation, Environmental ,Insect Proteins ,lcsh:Q - Abstract
Thermobia domestica belongs to an ancient group of insects and has a remarkable ability to digest crystalline cellulose without microbial assistance. By investigating the digestive proteome of Thermobia, we have identified over 20 members of an uncharacterized family of lytic polysaccharide monooxygenases (LPMOs). We show that this LPMO family spans across several clades of the Tree of Life, is of ancient origin, and was recruited by early arthropods with possible roles in remodeling endogenous chitin scaffolds during development and metamorphosis. Based on our in-depth characterization of Thermobia’s LPMOs, we propose that diversification of these enzymes toward cellulose digestion might have endowed ancestral insects with an effective biochemical apparatus for biomass degradation, allowing the early colonization of land during the Paleozoic Era. The vital role of LPMOs in modern agricultural pests and disease vectors offers new opportunities to help tackle global challenges in food security and the control of infectious diseases., LPMOs catalyze the oxidative breakdown of polysaccharides, thereby facilitating biomass degradation. By analyzing the digestive proteome of firebrats, the authors here identify a yet uncharacterized LPMO family and provide phylogenetic, structural and biochemical insights into its origin and functions.
- Published
- 2018