1. Extraction Methods for Quantitation of Gentamicin Residues from Tissues Using Fluorescence Polarization Immunoassay
- Author
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David R. Newkirk, Robert P. Hunter, S. A. Brown, Kyoko Sugimoto, and Gary G Smith
- Subjects
Kidney ,Chromatography ,medicine.diagnostic_test ,Coefficient of variation ,Renal cortex ,Aminoglycoside ,Skeletal muscle ,General Chemistry ,chemistry.chemical_compound ,medicine.anatomical_structure ,chemistry ,Immunoassay ,medicine ,Gentamicin ,Trichloroacetic acid ,medicine.drug - Abstract
Sodium hydroxide digestion of unhomogenlzed kidney and skeletal muscle for 20 min at 70°C was a superior method for extracting gentamicin from tissues, compared with simple homogenlzatlon, trichloroacetic acid precipitation of homogenized tissue, and sodium hydroxide digestion of homogenized tissue. Fluorescence polarization Immunoassay was used to quantltate gentamicin. Sodium hydroxide digestion of unhomogenlzed tissue allowed for the recovery of 90.0 ± 5.9% (Ẋ ± SD) from renal cortex and 79.9 ± 3.5% from skeletal muscle. The limit of sensitivity was 17.4 ng/g kidney tissue, 15.8 ng/g digested muscle, and 39.0 ng/g digested heart. The wlthin-assay coefficient of variation (CV) at 100 ng/g kidney was 9.2%; at 500 ng/g kidney, the CV was 2.5%; and at 2000 ng/g kidney, the CV was 1.5%. The between-assay coefficient of variation was
- Published
- 1990