1. The Extraction of Brain Isozymes with Solvents of Varying Polarity
- Author
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Jon J. Kabara and Daria Konvich
- Subjects
Male ,A lipoprotein ,Polarity (international relations) ,L-Lactate Dehydrogenase ,Chemistry ,Lipoproteins ,Electrophoresis, Starch Gel ,Extraction (chemistry) ,Esterases ,Brain ,Lactic dehydrogenase ,Electrophoresis, Disc ,Isozyme ,General Biochemistry, Genetics and Molecular Biology ,Catalysis ,Isoenzymes ,Mice ,Inorganic salts ,Nonspecific esterase ,Methods ,Solvents ,Animals ,Organic chemistry - Abstract
SummaryMouse brains were extracted with a variety of solutions composed of inorganic salts, organic solvents, or mixtures of each. During these experiments, it was noted that a differential effect of certain salts and solvents on isozyme patterns could be demonstrated. These effects of isozyme systems [lactic dehydrogenase (LDH) and nonspecific esterase] could best be explained on the basis of lipid-enzyme complexes. Circumstantial evidence is provided for viewing LDH as a lipoprotein catalyst.
- Published
- 1972
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