The Extraction of Brain Isozymes with Solvents of Varying Polarity

SummaryMouse brains were extracted with a variety of solutions composed of inorganic salts, organic solvents, or mixtures of each. During these experiments, it was noted that a differential effect of certain salts and solvents on isozyme patterns could be demonstrated. These effects of isozyme systems [lactic dehydrogenase (LDH) and nonspecific esterase] could best be explained on the basis of lipid-enzyme complexes. Circumstantial evidence is provided for viewing LDH as a lipoprotein catalyst.