Search

Se presenta el caso clínico de un paciente con Lipofuscinosis Neuronal Ceroidea tipo 2 (CLN2), condición genética producida por mutaciones en el gen TPP1. La CLN2 es una Enfermedad de Depósito Lisosomal (LSD), grupo de trastornos genéticos que forman parte de los Errores Innatos del Metabolismo, en los que se acumula un sustrato que no puede ser degradado a nivel de los lisosomas por la deficiencia en una enzima. En el caso de la CLN2, este déficit enzimático ocasiona un cuadro de deterioro neurológico acelerado con epilepsia refractaria, tratándose del primer caso descrito en la República de Panamá a la fecha. Esta condición es una enfermedad rara, y este reporte muestra la importancia de la necesidad de considerar la realización de paneles de diagnóstico genético cuando se presenta una epilepsia de difícil manejo en infantes.

From 2016 to 2018, six fungicide programs for the control of cucurbit powdery mildew ( Podosphaera xanthii) on a powdery mildew-resistant summer squash ‘Reward F1’ and powdery mildew-susceptible ‘Senator’ were evaluated with an emphasis on reducing the number of chlorothalonil applications. In all 3 years, cucurbit powdery control was greatest (based on lowest area under disease progress curve values) when fungicides with a high risk (HR) for resistance development and with different modes of action were applied in rotation all season or when HR fungicides were applied before and following the onset of disease development, rather than weekly following symptom observation in the susceptible and resistant cultivars. For a disease such as cucurbit powdery mildew, where there are multiple HR fungicides with different modes of action available and where their use could be limited to one or two applications per growing season, these fungicides might be suitable replacements for protectant fungicides in cucurbit crops that have stable genetic resistance to cucurbit powdery mildew.

Homogeneous catalysis applied to alcohol dehydration.

Fusarium wilt (FOB), caused by Fusarium oxysporum f. sp. basilici, remains an important disease of basil because of its persistence in the soil and seed transmission. Standardization of the methodology for investigating FOB resistance of basil cultivars is necessary for meaningful results. In a seed source experiment, six commercial seed sources of the sweet basil cultivar Nufar were tested in the greenhouse for their response to a single isolate of FOB at four inoculum concentrations (0, 102, 104, 106) at the six-leaf stage of growth. Differential susceptibility of the Nufar seed source lines was only revealed at a concentration of 104, but not at the lowest (102) or highest (106) concentrations. To understand the effects of inoculum concentration (0, 104, 105, 106), cultivar (Caesar, Nufar, RU172), and leaf stage (two, four, six) on FOB incidence and severity, a split-split plot experiment was conducted. There was a highly significant ( P < 0.0001) cultivar with inoculum concentration interaction effect on AUDPC, final plant height, and mortality. There were significant leaf stage with inoculum concentration interaction effects on AUDPC values ( P = 0.0093) and percentage of live plants ( P < 0.0001). There was a significant cultivar by leaf stage interaction effect ( P = 0.0006) on the final plant height. These results demonstrate that inoculum concentration and leaf stage interact to influence FOB incidence and severity. Based on our results, we recommend (i) screening at an intermediate inoculum concentration of 104 and a range of leaf stages; and (ii) culturing stem tissue from asymptomatic plants to detect latent infections.

OBJECTIVE To document ocular findings in cats with blastomycosis. ANIMALS 35 cats with blastomycosis. PROCEDURES Medical records from 1978 through 2019 were reviewed to identify cats with confirmed Blastomyces infection. Cats were grouped as having or not having ocular involvement. Clinical signs, histopathologic findings, and response to treatment were evaluated. RESULTS 21 of the 35 (60%) cats with confirmed blastomycosis had ocular abnormalities. Two of 21 cats with ocular abnormalities also had systemic hypertension and were excluded. Of the remaining 19 cats, 15 (79%) had bilateral ocular signs. Ten (53%) cats had inflammatory ocular lesions, and 9 (47%) had neuro-ophthalmic abnormalities. Six of the 19 (32%) cats appeared to be completely blind, and 5 (26%) appeared to be unilaterally blind. For the 10 cats with inflammatory ocular lesions, the most common lesions were anterior uveitis (9/20 eyes), active chorioretinitis (6/20 eyes), and retinal detachment (4/20 eyes). For the 9 cats with neuro-ophthalmic abnormalities, the most common abnormalities were a negative menace or tracking response (10/18 eyes) and negative pupillary light response (4/18 eyes). CLINICAL RELEVANCE Results suggested that ocular involvement is common in cats with blastomycosis and that both inflammatory lesions and neuro-ophthalmic abnormalities can be seen. Blastomycosis should be considered in the differential diagnosis for cats with anterior uveitis, posterior segment inflammation, or neuro-ophthalmic abnormalities, and a complete ophthalmic examination should be performed in all cats with confirmed or suspected blastomycosis.

Background As demand for primary total joint arthroplasty (TJA) continues to grow, a proportionate increase in revision TJA (rTJA) is expected. It is essential to understand costs and reimbursement of rTJA as our country moves to bundled payment models. We aimed (1) to characterize implant and total hospital costs, (2) assess reimbursement, and (3) determine revenue for rTJA in comparison with primary TJA. Methods The average implant and total hospital cost of all primary and rTJA procedures by diagnosis-related group (DRG) was calculated using time-driven activity-based costing at an orthopedic hospital from 2018 to 2020. Average reimbursement and payer type were assessed by DRG. Revenue was calculated by deducting average time-driven activity-based costing total costs from reimbursement. Results 13,946 arthroplasties were included in the study. Implant cost comprised 55.8% of total hospital costs for rTJA DRG 468, compared with 43.6% of total hospital costs for primary TJA DRG 470. Total hospital costs for DRG 468 were 61.1% more than DRG 470. Reimbursement for rTJA was 1.23x more than primary TJA. Private payers paid 23.2% more than Medicare for rTJA. Margin for DRG 468 was 1.5% less than primary DRG 470. Conclusion rTJA requires more hospital resources and costs than primaries, yet hospital reimbursement may be inadequate with the additional expenditures necessary to provide optimal care. If hospitals cannot perform revision services under the current reimbursement model, patient access may be limited. Implant costs are a major contributor to overall rTJA cost. Strategies are needed to reduce revision implant costs to improve value of care. Level of Evidence Level III, economic and decision analysis.

In collaboration with the American College of Veterinary Pathologists

OBJECTIVE To report clinical characteristics of dogs with bacterial keratitis, identify the most common bacterial isolates within this population, characterize the bacterial isolates' antimicrobial resistance patterns, and compare those resistance patterns to previously reported resistance patterns. ANIMAL STUDIED Dogs diagnosed with bacterial keratitis between 2013 and 2019. PROCEDURES Data pertaining to breed; Schirmer tear test I results; use of cyclosporine, tacrolimus, or corticosteroids at time of ulcer diagnosis; bacterial genus or species isolated; and resistance to selected antimicrobials as measured by Kirby-Bauer disk diffusion were collected. Resistance patterns were compared to those reported by Tolar et al. (2006). RESULTS One hundred seventy bacterial isolates were cultured from 138 eyes from 130 dogs. Of these dogs, 45% were brachycephalic, 62% had STT

Background: The superior approach for total hip arthroplasty (THA) is a minimally invasive, tissue-sparing technique that may have clinical and economic benefits. The purpose of this study was to compare early outcomes between the posterior approach and the superior approach in primary THA. Our hypothesis was that the superior approach would have a noninferior length of stay (LOS), discharge destination, and blood loss compared with the posterior approach. Methods: All primary THAs performed by a single surgeon at one institution were retrospectively reviewed over a 2-year period (2015-2017). There were 676 patients, 40.4% of whom underwent a posterior approach and 59.6% underwent a superior approach. LOS, discharge destination, blood loss, and operating room time were analyzed. Gender, body mass index, and American Society of Anesthesiologists status were recorded and controlled. Results: The posterior approach was independently associated with an almost threefold higher risk of prolonged LOS (>2 days, P < .001) (odds ratio: 2.90, 95% confidence interval: 1.87-4.49; P < .001). The mean LOS for the superior approach was 1.71 days vs 2.17 days for the posterior group (P < .001). Fewer patients in the superior approach cohort were discharged to a rehabilitation facility (8.9% vs 17.9%, P < .001). The mean operative time was shorter in the superior group (91.8 vs 95.8, P = .001). There was no statistically significant difference in acute postoperative blood loss. There were no dislocations or reoperations in either group. Conclusions: The superior approach to THA was associated with a significantly shorter length of hospital stay and lower rate of discharge to rehab than the posterior approach. This approach can be used as a safe, minimally invasive, and tissue-sparing variation of a standard posterior approach for THA and has promising early outcomes.

Hydrogen lithography has been used to template phosphine-based surface chemistry to fabricate atomic-scale devices, a process we abbreviate as atomic precision advanced manufacturing (APAM). Here, we use mid-infrared variable angle spectroscopic ellipsometry (IR-VASE) to characterize single-nanometer thickness phosphorus dopant layers (δ-layers) in silicon made using APAM compatible processes. A large Drude response is directly attributable to the δ-layer and can be used for nondestructive monitoring of the condition of the APAM layer when integrating additional processing steps. The carrier density and mobility extracted from our room temperature IR-VASE measurements are consistent with cryogenic magneto-transport measurements, showing that APAM δ-layers function at room temperature. Finally, the permittivity extracted from these measurements shows that the doping in the APAM δ-layers is so large that their low-frequency in-plane response is reminiscent of a silicide. However, there is no indication of a plasma resonance, likely due to reduced dimensionality and/or low scattering lifetime.

Objective To determine the tear film levels of oxytetracycline in normal canine eyes after application of the ophthalmic ointment, Terramycin™ (0.5% oxytetracycline, polymyxin B sulfate), to guide appropriate treatment frequency. Animals studied Ten research beagles. Procedures Ten research beagles with confirmed normal eyes were administered 0.02 mL of Terramycin™ ophthalmic ointment onto the dorsal bulbar conjunctival surface of the right eye. Tear samples were collected via dye-less Schirmer tear strips at 2, 4, 6, 8, and 12 hours post-administration. The sample for each timepoint was collected on a separate day, and concentrations of oxytetracycline were determined using high-performance liquid chromatography (HPLC). Results There was a semi-logarithmic decline in the median tear concentration of oxytetracycline. The median (2.5th and 97.5th percentiles) tear concentrations of oxytetracycline at 2, 4, 6, 8, and 12 hours were 43.5 μg/mL (11.1-302.2 μg/mL), 28.7 μg/mL (8.04-113.7 μg/mL), 16.1 μg/mL (4.96-37.7 μg/mL), 9.2 μg/mL (4.52-28.1 μg/mL), and 6.11 μg/mL (4.36-26.7 μg/mL), respectively. Mean (±SD) drug recovery via HPLC was 88% (±7.5%). Conclusions Ophthalmic Terramycin™ achieves a substantially higher tear level than the MIC for common bacterial corneal pathogens up to 12 hours post-administration in normal eyes. Anti-collagenolytic tear levels were not achieved at the timepoints evaluated or with the manufacturer-prescribed dosing frequency. HPLC can be used to analyze tear concentrations of ophthalmic ointment formulations.

Objective To assess lens capsule opacity (LCO) following phacoemulsification in dogs receiving long-term topical ophthalmic flurbiprofen sodium 0.03%. Animal studied Twenty-five client owned dogs undergoing phacoemulsification surgery for either diabetic or nondiabetic cataracts. Methods Prospective, randomized, masked clinical study assessing two groups of dogs for twelve months following phacoemulsification. All dogs underwent a complete eye examination and were photographed at each visit, beginning three weeks post-surgery, and repeated at 2, 3, 6, and 12 months post-surgery. Post-operative treatment protocols were similar for both groups, except that Group F received topical flurbiprofen once daily for 12 months and Group A received artificial tears once daily for 12 months. Digital photographs were analyzed for LCO using a subjective grading scale (0-4). The change in capsular opacities from 3 weeks to 12 months post-surgery was evaluated for each dog, and the groups were compared. Results There were 25 dogs evaluated post-phacoemulsification at each designated time point of the study. There were 12 dogs in Group A and 13 dogs in Group F. There was no significant statistical difference in LCO score between Groups A and F at baseline (3 weeks post-surgery), 6 months post-surgery, or 12 months post-surgery. Scores did not change significantly from baseline to 12 months within or between groups. Conclusions Topical ophthalmic flurbiprofen sodium 0.03% solution applied once daily for 12 months following phacoemulsification does not appear to lead to a decrease in LCO formation as compared to artificial tears control.

Scanning tunneling microscope lithography can be used to create nanoelectronic devices in which dopant atoms are precisely positioned in a $\mathrm{Si}$ lattice within approximately $1$ nm of a target position. This exquisite precision is promising for realizing various quantum technologies. However, a potentially impactful form of disorder is due to incorporation kinetics, in which the number of P atoms that incorporate into a single lithographic window is manifestly uncertain. We present experimental results indicating that the likelihood of incorporating into an ideally written three-dimer single-donor window is $63\ifmmode\pm\else\textpm\fi{}10\mathrm{%}$ for room-temperature dosing, and corroborate these results with a model for the incorporation kinetics. Nevertheless, further analysis of this model suggests conditions that might raise the incorporation rate to near-deterministic levels. We simulate bias spectroscopy on a chain of comparable dimensions to the array in our yield study, indicating that such an experiment may help confirm the inferred incorporation rate.

As transistor features shrink beyond the 2 nm node, studying and designing for atomic scale effects become essential. Being able to combine conventional CMOS with new atomic scale fabrication routes capable of creating 2D patterns of highly doped phosphorus layers with atomic precision has implications for the future of digital electronics. This work establishes the accelerated lifetime tests of such doped layers, showing that these materials survive high current (>3.0 MA/cm2) and 300$^{\circ}$C for greater than 70 days and are still electrically conductive. The doped layers compare well to failures in traditional metal layers like aluminum and copper where mean time to failure at these temperatures and current densities would occur within hours. It also establishes that these materials are more stable than metal features, paving the way toward their integration with operational CMOS., In IEEE Trans. Dev. Mater. Rel. (2022). Copyright 2022 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, including reprinting/republishing this material for advertising or promotional purposes, collecting new collected works for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works

2021 is the 50th anniversary of the FDA approval of minocycline (MCN). While many other antibiotics have become obsolete during this time, MCN continues to be quite useful. In dermatology, MCN is used prominently in acne vulgaris, and is also employed in many other dermatological conditions because of its molecular and pharmacological properties. In this article, we review the history of minocycline, and outline the evolution of the drug since its inception. Based on its existing longstanding utility and continued innovations in formulation and delivery systems, we postulate that it will continue to have a prominent position in the dermatologistrsquo;s armamentarium. J Drugs Dermatol. 2021;20(10):1031-1036. doi:10.36849/JDD.6370.

Blueberry plants displaying abnormal fruit development in the form of splitting and equatorial russeting were reported from 15 farms in Atlantic and Burlington Counties, NJ, in the spring of 2017. The cause was unknown; however, phytotoxicity due to a chemical application was suspected. Grower application records were used to unravel the cause. A total of 237 records were received, of which 110 were from fields displaying injury. A logistic regression analysis identified pendimethalin as the primary cause, although soil type did show a probability greater than 1. Other incriminated herbicides were frequent tank mix partners with pendimethalin and not responsible for injury. The methodology described is helpful for identifying explanatory factors among a complex set of possible or likely causative elements. In this case pendimethalin was identified as the cause of blueberry phytotoxicity.

Background: Dual-modular femoral components with exchangeable cobalt-chrome neck segments have had higher than expected failure rates due to corrosion and adverse local tissue reaction (ALTR). Complications, survival rates and early clinical outcomes of revision surgery for the treatment of corrosion and ALTR as a result of these implants are underreported. Methods: We identified 44 cases of revision THA for corrosion and ALTR resulting from the same dual modular stem. All revision procedures were performed using a modular tapered fluted titanium stem, ceramic heads and highly cross-linked polyethylene. Results: Complications included: dislocation, infection, reoperation, and chronic pain. Mean Harris Hip Score was 84 following revision surgery. Conclusions: Patients undergoing revision surgery for ALTR related to this prosthesis should be aware of the risk of postoperative dislocation and other complications and the potential long-term risk of some chronic pain.

Climate change is of major relevance to wine production as most of the winegrowing regions of the world are located within relatively narrow latitudinal bands with average growing-season temperatures (GSTs) limited to 13–21 C. This study focuses on the incidence of climate variables and indices that are relevant both for climate change assessment and for grape production, with emphasis on grapevine bioclimatic indices and extreme events (e.g., cold waves, storms, heatwaves). Dynamical downscaling of European Reanalysis-Interim and Max Planck Institute Earth System low-resolution global simulations forced with a Representative Concentration Pathway 8.5 (RCP8.5) greenhouse gas emission scenario was performed with the Weather Research and Forecast (WRF) model to a regional scale including the Douro Valley of Portugal for recent-past (1986–2005) and future periods (2046–2065, 2081–2100). The number, duration and intensity of events were superimposed over critical phenological phases estimated by using a specific local grapevine varietal phenological model in order to assess their positive or negative implications for wine production in the region. An assessment of the relevance of climate parameters and indices and their progression in recent-past and future climate scenarios with regard to the potential impact on wine production was performed. Results indicate a positive relation between higher growing-season heat accumulations and greater vintage yields. A moderate incidence of very hot days (daily maximum temperature above 35 C) and drought from pre-véraison phenological conditions have a positive association with vintage ratings. However, the mid- and long-term WRF-MPI RCP8.5 future climate scenarios reveal shifts to warmer and drier conditions, with the mean GST not remaining within range for quality wine production in the long-term future climate scenario. These results indicate potential impacts that suggest a range of strategies to maintain wine production and quality in the region. The authors wish to thank the DOUROZONE project (PTDC/AAG-MAA/3335/2014; POCI-01-0145-FEDER- 016778) for financial support through Project 3599 – Promoting the Scientific Production and the Technological Development, and Thematic Networks (3599-PPCDT) – and through FEDER, and the national funds from FCT-Science and Technology Portuguese Foundation for the doctoral grant of D. Blanco-Ward (SFRH/BD/139193/2018). Thanks are also due for the financial support to CESAM (UID/AMB/50017 - POCI-01-0145-FEDER-007638), to FCT/MEC through national funds, and the co-funding by FEDER within the PT2020 Partnership Agreement and Compete 2020. info:eu-repo/semantics/publishedVersion

Background:Individual pelvic tilt and rotation have wide variability that can affect the measurement of cup orientation in anteroposterior (AP) radiographs. The purpose of this study was to analyse the effect of pelvic tilt and rotation on radiographic measurements of cup orientation.Methods:A total of 53 patients (63 hips) were included in this study. The patients underwent a computed tomography study with standing AP pelvis radiographs taken both preoperatively and approximately 3 months postoperatively. We used 2-dimensional/3-dimensional matching to measure the pelvic tilt and rotation, and the non-standardised and standardised cup orientation.Results:There was no difference in the pelvic tilt and rotation between the preoperative and postoperative radiographs. The distribution of the differences between the non-standardised and standardised cup anteversion exhibited a change within 5° in only 34/63 (54%) hips. The pelvic tilt correlated with the difference between the non-standardised and standardised cup anteversion, but the pelvic rotation did not. When all 63 hips were separated into the right and left sides, the pelvic rotation inversely correlated with the pelvic tilt-adjusted difference between the non-standardised and standardised cup anteversion of the right side but directly correlated with that of the left side.Conclusions:The current study demonstrated that the measurement of cup anteversion in standing AP radiographs is significantly affected by both the pelvic tilt and pelvic rotation. An improved understanding of the pelvic orientation may eventually allow for desired cup positioning on a patient-specific basis to potentially reduce complications associated with the malposition of the cup.

The attachment of dopant precursor molecules to depassivated areas of hydrogen-terminated silicon templated with a scanning tunneling microscope (STM) has been used to create electronic devices with subnanometer precision, typically for quantum physics experiments. This process, which we call atomic precision advanced manufacturing (APAM), dopes silicon beyond the solid-solubility limit and produces electrical and optical characteristics that may also be useful for microelectronic and plasmonic applications. However, scanned probe lithography lacks the throughput required to develop more sophisticated applications. Here, we demonstrate and characterize an APAM device workflow where scanned probe lithography of the atomic layer resist has been replaced by photolithography. An ultraviolet laser is shown to locally and controllably heat silicon above the temperature required for hydrogen depassivation on a nanosecond timescale, a process resistant to under- and overexposure. STM images indicate a narrow range of energy density where the surface is both depassivated and undamaged. Modeling that accounts for photothermal heating and the subsequent hydrogen desorption kinetics suggests that the silicon surface temperatures reached in our patterning process exceed those required for hydrogen removal in temperature-programmed desorption experiments. A phosphorus-doped van der Pauw structure made by sequentially photodepassivating a predefined area and then exposing it to phosphine is found to have a similar mobility and higher carrier density compared with devices patterned by STM. Lastly, it is also demonstrated that photodepassivation and precursor exposure steps may be performed concomitantly, a potential route to enabling APAM outside of ultrahigh vacuum.

Spin-orbit effects, inherent to electrons confined in quantum dots at a silicon heterointerface, provide a means to control electron spin qubits without the added complexity of on-chip, nanofabricated micromagnets or nearby coplanar striplines. Here, we demonstrate a singlet-triplet qubit operating mode that can drive qubit evolution at frequencies in excess of 200 MHz. This approach offers a means to electrically turn on and off fast control, while providing high logic gate orthogonality and long qubit dephasing times. We utilize this operational mode for dynamical decoupling experiments to probe the charge noise power spectrum in a silicon metal-oxide-semiconductor double quantum dot. In addition, we assess qubit frequency drift over longer timescales to capture low-frequency noise. We present the charge noise power spectral density up to 3 MHz, which exhibits a 1/f

Spin-orbit effects, inherent to electrons confined in quantum dots at a silicon heterointerface, provide a means to control electron spin qubits without the added complexity of on-chip, nanofabricated micromagnets or nearby coplanar striplines. Here, we demonstrate a novel singlet-triplet qubit operating mode that can drive qubit evolution at frequencies in excess of 200 MHz. This approach offers a means to electrically turn on and off fast control, while providing high logic gate orthogonality and long qubit dephasing times. We utilize this operational mode for dynamical decoupling experiments to probe the charge noise power spectrum in a silicon metal-oxide-semiconductor double quantum dot. In addition, we assess qubit frequency drift over longer timescales to capture low-frequency noise. We present the charge noise power spectral density up to 3 MHz, which exhibits a $1/f^{\alpha}$ dependence consistent with $\alpha \sim 0.7$, over 9 orders of magnitude in noise frequency., Comment: Supplementary information included with the paper

The grapevine (Vitis vinifera L.) is a crop with great cultural, economic and ecological relevance for Mediterranean environments besides being the fruit crop with largest acreage and economic value at the global scale. Its exposure to high levels of tropospheric ozone (O3) can result in phytotoxic effects and thus it is important to comprehensively re-evaluate these effects as well as related processes. A review of the validity and limitations of the standards used for the protection of vegetation in relation to ambient O3, the state-of the-art knowledge on O3 phytotoxic effects on the grapevine and the available means to assess its impact are presented and discussed. It is concluded that wide regions in the world, mainly between latitudes 30◦ and 50◦ N, where the grapevine has been traditionally cultivated, are exposed to O3 concentrations that can affect both the yield and quality of the grape. Recently reported studies for global cultivars such as Cabernet Sauvignon or Merlot, along publicly available maps on O3 standards to protect vegetation at the European and global scale, indicate potential yield reductions in the range of 20–31% and the quality of the grape can also be affected by reductions of total polyphenols in the range of 15–23% for these areas. Although a tendency to reduce ambient O3 levels has been registered since 2000 in the western European Mediterranean basin, the flux of O3 into the grapevine leaves could still exceed critical levels with phenological advancement driven by the increase of temperatures or interaction between O3 and other climatic variables such as drought or high summer light intensities. Higher O3 exposures are reported in western United States of America and eastern China, with this last region maintaining an increasing tendency in summer ambient O3 levels. It is still necessary to adopt common experimental and monitoring protocols to establish grapevine-specific O3 relationships and critical levels, as there is not yet a coherent and shared database for detailed risk assessment for this crop. The authors wish to thank the national funds from FCT-Science and Technology Portuguese Foundation for the doctoral grant of D. Blanco- Ward (SFRH/BD/139193/2018). Thanks, are also due for the financial support to CESAM (UID/AMB/50017 - POCI-01-0145-FEDER-007638), to FCT/MEC through national funds, and the co-funding by FEDER within the PT2020 Partnership Agreement and Compete 2020. The authors wish to thank the DOUROZONE project (PTDC/AAG-MAA/3335/2014; POCI-01-0145-FEDER-016778) for financial support through Project 3599 – Promoting the Scientific Production and the Technological Development, and Thematic Networks (3599-PPCDT) – and through FEDER. info:eu-repo/semantics/publishedVersion

Desert dust aerosols affect Earth’s global energy balance through interactions with radiation(1,2), clouds(3,4), and ecosystems(5). But the magnitudes of these effects are so uncertain that it remains unclear whether atmospheric dust has a net warming or cooling effect on global climate(1,4,6). Consequently, it is still uncertain whether large changes in atmospheric dust loading over the past century have slowed or accelerated anthropogenic climate change(4,7–9), and the climate impact of possible future alterations in dust loading is similarly disputed(9,10). Here we use an integrative analysis of dust aerosol sizes and abundance to constrain the climatic impact of dust through direct interactions with radiation. Using a combination of observational, experimental, and model data, we find that atmospheric dust is substantially coarser than represented in current climate models. Since coarse dust warms global climate, the dust direct radiative effect (DRE) is likely less cooling than the ~0.4 W/m(2) estimated by models in a current ensemble(2,11–13). We constrain the dust DRE to - 0.20 (−0.48 to +0.20) W/m(2), which suggests that the dust DRE produces only about half the cooling that current models estimate, and raises the possibility that dust DRE is actually net warming the planet.

The attachment of dopant precursor molecules to depassivated areas of hydrogen-terminated silicon templated with a scanning tunneling microscope (STM) has been used to create electronic devices with sub-nanometer precision, typically for quantum physics demonstrations, and to dope silicon past the solid-solubility limit, with potential applications in microelectronics and plasmonics. However, this process, which we call atomic precision advanced manufacturing (APAM), currently lacks the throughput required to develop sophisticated applications because there is no proven scalable hydrogen lithography pathway. Here, we demonstrate and characterize an APAM device workflow where STM lithography has been replaced with photolithography. An ultraviolet laser is shown to locally heat silicon controllably above the temperature required for hydrogen depassivation. STM images indicate a narrow range of laser energy density where hydrogen has been depassivated, and the surface remains well-ordered. A model for photothermal heating of silicon predicts a local temperature which is consistent with atomic-scale STM images of the photo-patterned regions. Finally, a simple device made by exposing photo-depassivated silicon to phosphine is found to have a carrier density and mobility similar to that produced by similar devices patterned by STM.

One major aim of preclinical intracerebral hemorrhage (ICH) research is to develop and test potential neuroprotectants. Published guidelines for experimental design and reporting stress the importance of clearly and completely reporting results and methodological details to ensure reproducibility and maximize information availability. The current review has two objectives: first, to characterize current ICH neuroprotection research and, second, to analyze aspects of translational design in preclinical ICH studies. Translational design is the adoption and reporting of experimental design characteristics that are thought to be clinically relevant and critical to reproducibility in animal studies (e.g., conducting and reporting experiments according to the STAIR and ARRIVE guidelines, respectively). Given that ICH has no current neuroprotective treatments and an ongoing reproducibility crisis in preclinical research, translational design should be considered by investigators. We conducted a systematic review of ICH research from 2015 to 2019 using the PubMed database. Our search returned 281 published manuscripts studying putative neuroprotectants in animal models. Contemporary ICH research predominantly uses young, healthy male rodents. The collagenase model is the most commonly used. Reporting of group sizes, blinding, and randomization are almost unanimous, but group size calculations, mortality and exclusion criteria, and animal model characteristics are infrequently reported. Overall, current ICH neuroprotection research somewhat aligns with experimental design and reporting guidelines. However, there are areas for improvement. Because failure to consider translational design is associated with inflation of effect sizes (and possibly hindered reproducibility), we suggest that researchers, editors, and publishers collaboratively consider enhanced adherence to published guidelines.

A 2-year-old, male castrated German shepherd dog was presented to the University of Tennessee Veterinary Medical Center (UTVMC) with periorbital swelling and conjunctival mucopurulent discharge 2 days following removal of a twig from the medial canthus by the owner. Diagnostic imaging was pursued due to the suspicion of a retrobulbar foreign body (FB). A cylindrical FB approximately 3.0 cm in length and 1.0 cm in diameter with concentric rings, suspected to be wooden material, was identified on computed tomography (CT) imaging. An attempt to remove the FB via a stab incision using ultrasound guidance was unsuccessful, and postmanipulation ultrasound confirmed the FB position was unchanged. An exploratory orbitotomy was performed, using the acquired CT images for guidance in locating the FB; however, the FB was not present at the predicted site. The CT imaging was repeated and showed that the FB had migrated rostrally approximately 3.0 cm, compared to the originally acquired study and its same location during attempted ultrasound-guided removal. A combination of CT-guided needle placement and contrast injection was then used with repeat imaging in an attempt to better localize the FB and its soft tissue tract. The dog was taken back into the operating room, and the wooden FB was successfully removed.

The purpose of this study is to analyze the effect of pelvic tilt and rotation on radiographic measurement of cup orientation.A total of 68 patients (79 hips) were included in this study. The patients had a computed tomography study and approximately 3 months of postoperative standing anteroposterior pelvic radiographs in both supine and standing positions. We used 2-dimensional (2D)/3-dimensional (3D) matching to measure pelvic tilt and rotation, and cup orientation.There was a wide range of pelvic tilt between individuals in both supine and standing positions. Supine pelvic tilt was different from standing pelvic tilt (P.05). There were differences in cup anteversion before and after 2D/3D matching in both supine and standing positions (P.05). Supine and standing pelvic tilt correlated with differences in cup anteversion before and after 2D/3D matching. When all 79 hips were separated into right and left side, pelvic rotation inversely correlated with the pelvic tilt-adjusted difference in anteversion before and after 2D/3D matching of the right side but directly correlated with that of the left side in both supine and standing positions.This study demonstrated that the measurement of cup anteversion on anteroposterior radiographs is significantly affected by both pelvic tilt and pelvic rotation (depending on the side). Improved understanding of pelvic orientation and improved ability to measure pelvic orientation may eventually allow for desired cup positioning to potentially protect against complications associated with malposition of the cup.

OBJECTIVES To compare visual outcome and cause of blindness between two groups of dogs undergoing phacoemulsification. ANIMALS STUDIED Study population consisted of 35 Boston terriers (BT; 70 eyes) and 77 non-Boston terriers (NBT; 154 eyes) that underwent bilateral phacoemulsification surgery. PROCEDURES Medical records were reviewed to determine visual outcome, complications leading to blindness and follow-up. A Kaplan-Meier survival curve was used to estimate surgical success at 1 and 2 years postoperatively. RESULTS Eleven of 35 BT developed known vision loss, with 12 of 70 (17.1%) eyes becoming blind within one year of surgery, and 17 of 77 NBT developed known vision loss, with 19 of 154 (12.3%) eyes becoming blind within 1 year of surgery. No further vision loss was reported among those BT with a 2-year follow-up. In the NBT group, seven additional dogs, a total of 24 of 77 NBT developed vision loss, with a known total of 31 of 154 (20.1%) eyes becoming blind within two years of surgery. These results were not significantly different. The overall surgical success in both groups based on individual eyes was estimated to be between 80 and 90% at 1 year postsurgery and between 65 and 80% at 2 years postsurgery. Glaucoma was the leading cause of vision loss in both groups. CONCLUSIONS There was no significant difference in visual outcomes between a group of BT and a group of NBT undergoing bilateral phacoemulsification at the University of Tennessee between 2002 and 2015. Glaucoma was the leading cause of vision loss in both groups.