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180 results on '"Dai, Liangying"'

4. The nematode effector calreticulin competes with the high mobility group protein OsHMGB1 for binding to the rice calmodulin‐like protein OsCML31 to enhance rice susceptibility to Meloidogyne graminicola

Author

Liu, Jing, primary, Zhang, Jiaqian, additional, Wei, Ying, additional, Su, Wen, additional, Li, Wei, additional, Wang, Bing, additional, Peng, Deliang, additional, Gheysen, Godelieve, additional, Peng, Huan, additional, and Dai, Liangying, additional

Published
2024
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11. Citrus Huanglongbing correlated with incidence of Diaphorina citri carrying Candidatus Liberibacter asiaticus and citrus phyllosphere microbiome

Author

Hu, Yang, primary, Meng, Youqing, additional, Yao, Liangjin, additional, Wang, Enguo, additional, Tang, Tao, additional, Wang, Yunsheng, additional, Dai, Liangying, additional, Zhao, Mingping, additional, Zhang, Hong-en, additional, Fan, Xiaoyan, additional, Luo, Luyun, additional, Xiang, Wei, additional, and Zhang, Zhuo, additional

Published
2022
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19. Integrated Proteomics and Transcriptomics Analyses Reveal the Transcriptional Slippage of P3N-PIPO in a Bymovirus

Author

Yu, Chulang, Miao, Runpu, Ye, Zhuangxin, MacFarlane, Stuart, Lu, Yuwen, Li, Junmin, Yang, Jian, Yan, Fei, Dai, Liangying, and Chen, Jianping

Subjects
Bymovirus, P3N-PIPO, transcriptional slippage, PVX
Abstract

P3N-PIPO (P3 N-terminal fused with Pretty Interesting Potyviridae ORF), the movement protein of potyviruses, is expressed as a translational fusion with the N-terminus of P3 in potyviruses. As reported in previous studies, P3N-PIPO is expressed via transcriptional slippage at a conserved G2A6 slippery site in the genus Potyvirus. However, it is still unknown whether a similar expression mechanism of P3N-PIPO is used in the other genera of the family Potyviridae. Moreover, due to the extremely low expression level of P3N-PIPO in natural virus-infected plants, the peptides spanning the slippery site which provide direct evidence of the slippage at the protein level, have not been identified yet. In this study, a potato virus X (PVX)-based expression vector was utilized to investigate the expression mechanism of P3N-PIPO. A high expression level of the P3N-PIPO(WT) of turnip mosaic virus (TuMV, genus Potyvirus) was observed based on the PVX expression vector. For the first time, we successfully identified the peptides of P3N-PIPO spanning the slippery site by mass spectrometry. Likewise, the P3N-PIPO(WT) of wheat yellow mosaic virus (WYMV, genus Bymovirus) was also successfully expressed using the PVX expression vector. Integrated proteome and transcriptome analyses revealed that WYMV P3N-PIPO was expressed at the conserved G2A6 site through transcriptional slippage. Moreover, as revealed by mutagenesis analysis, Hexa-adenosine of the G2A6 site was important for the frameshift expression of P3N-PIPO in WYMV. According to our results, the PVX-based expression vector might be used as an excellent tool to study the expression mechanism of P3N-PIPO in Potyviridae. To the best of our knowledge, this is the first experimental evidence for the expression mechanism of P3N-PIPO in the genus Bymovirus, the only genus comprising bipartite virus species in the family Potyviridae.

Published
2021
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21. Enhancement of edeine production in Brevibacillus brevis X23 via in situ promoter engineering

Author

Liu, Qingshu, primary, Zhang, Liang, additional, Wang, Yunsheng, additional, Zhang, Cuiyang, additional, Liu, Tianbo, additional, Duan, Caichen, additional, Bian, Xiaoying, additional, Guo, Zhaohui, additional, Long, Qingshan, additional, Tang, Ying, additional, Du, Jie, additional, Liu, Aiyu, additional, Dai, Liangying, additional, Li, Dingjun, additional, and Chen, Wu, additional

Published
2021
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37. The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice

Author

Qu, Shaohong, Liu, Guifu, Zhou, Bo, Bellizzi, Maria, Zeng, Lirong, Dai, Liangying, Han, Bin, and Wang, Guo-Liang

Subjects
Proteins -- Genetic aspects, Proteins -- Research, Genetic research -- Reports, Biological sciences
Abstract

The broad-spectrum rice blast resistance gene Pi9 was cloned using a map-based cloning strategy. Sequencing of a 76-kb bacterial artificial chromosome (BAC) contig spanning the Pi9 locus led to identification of six tandemly arranged resistance-like genes with a nucleotide-binding site (NBS) and leucine-rich repeats (LRRs) (Nbs1-Pig--Nbs6-Pi9). Analysis of selected Pi9 deletion mutants and transformation of a 45-kb fragment from the BAC contig into the susceptible rice cultivar TP309 narrowed down Pi9 to the candidate genes Nbs2-Pi9 and Nbs3-Pi9. Disease evaluation of the transgenic lines carrying the individual candidate genes confirmed that Nbs2-Pi9 is the Pi9 gene. Sequence comparison analysis revealed that the six paralogs at the Pi9 locus belong to four classes and gene duplication might be one of the major evolutionary forces contributing to the formation of the NBS--LRR gene cluster. Semiquantitative reverse transcriptase (RT)-PCR analysis showed that Pi9 was constitutively expressed in the Pi9-resistant plants and was not induced by blast infection. The cloned Pi9 gene provides a starting point to elucidate the molecular basis of the broadspectrum disease resistance and the evolutionary mechanisms of blast resistance gene clusters in rice.

Published
2006

41. Enhancement of edeine production in Brevibacillus brevis X23 via in situ promoter engineering.

Author

Liu, Qingshu, Zhang, Liang, Wang, Yunsheng, Zhang, Cuiyang, Liu, Tianbo, Duan, Caichen, Bian, Xiaoying, Guo, Zhaohui, Long, Qingshan, Tang, Ying, Du, Jie, Liu, Aiyu, Dai, Liangying, Li, Dingjun, and Chen, Wu

Subjects
ANTIMICROBIAL peptides, GENETIC engineering, GENE clusters, SOIL microbiology, CHEMICAL synthesis
Abstract

Summary: Edeines, a group of cationic antimicrobial peptides produced by the soil bacterium Brevibacillus, have broad biological effects, such as antimicrobial, anticancer and immunosuppressive activities. However, the yield of edeines in wild‐type (WT) Brevibacillus is extremely low, and chemical synthesis of edeines is a time‐consuming process. Genetic engineering has proven to be an effective approach to produce antibiotics with high yield. In this study, the edeine biosynthetic gene cluster (ede BGC), which is involved in edeine production, was identified and characterized in Brevibacillus brevis X23. To improve edeine production in B. brevis X23, the ede BGC promoter was replaced with six different promoters, Pmwp, Pspc, PxylA, Pshuttle‐09, Pgrac or P43, through double‐crossover homologous recombination. The new promoters significantly increased the expression of the ede BGC as well as edeine production by 2.9 ± 0.4 to 20.5 ± 1.2‐fold and 3.6 ± 0.1to 8.7 ± 0.7‐fold respectively. The highest yield of edeines (83.6 mg l−1) was obtained in B. brevis X23 with the Pmwp promoter. This study provides a practical approach for producing high yields of edeines in B. brevis. [ABSTRACT FROM AUTHOR]

Published
2022
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42. Identification and optimization of biological bacteria against tobacco mosaic virus(TMV)through fermentation.

Author

ZHAO Yuqiang, ZHU Sanrong, LIU Tianbo, TENG Kai, CAI Hailin, ZHOU Xiangping, ZENG Weiai, YANG Hongwu, DAI Liangying, ZHOU Zhicheng, ZHOU Peng, and TANG Qianjun

Abstract

In order to effectively prevent and control the disease caused by common tobacco mosaic virus (TMV), a bacterium strain, Z5, resistant to TMV was isolated from soil and was identified as Bacillus velezensis through morphological, physiological, biochemical and molecular biological tests. The culture solution of this bacterium had an in vitro inhibition rate against TMV up to 96.40% and a control effect of 50.43% on TMV in field plot experiments. After the optimization of fermentation condition through a single factor experiment, the optimal medium for strain Z5 was NB medium with glucose and yeast extract as carbon and nitrogen sources respectively, and the optimum conditions were initial pH 7.0, medium volume 100 mL/250mL and fermentation at 28 ° for 60-72 h. Under the optimized condition, the bacterial count increased by 44.38% and the fermentation broth had a stable inhibitory effect against TMV with an inhibition rate of 98.71%. [ABSTRACT FROM AUTHOR]

Published
2022
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45. Solar thermotherapy reduces the titer of Candidatus Liberibacter asiaticus and enhances canopy growth by altering gene expression profiles in HLB-affected citrus plants

Author

Doud, Melissa M, primary, Wang, Yungsheng, additional, Hoffman, Michelle T, additional, Latza, Christina L, additional, Luo, Weiqi, additional, Armstrong, Cheryl M, additional, Gottwald, Tim R, additional, Dai, Liangying, additional, Luo, Feng, additional, and Duan, Yongping, additional

Published
2017
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46. Transformation of the BWMK1-interacting gene BWIP4

Author

Hu YaJun, Liu Ling, Gao Jia, Pan SuJun, Liu Xionglun, Dai LiangYing, and Wang Guo-Liang

Subjects
Reverse transcription polymerase chain reaction, Genetics, Transformation (genetics), biology, Agrobacterium, RNA interference, Transgene, fungi, food and beverages, GUS reporter system, Genetically modified crops, biology.organism_classification, Gene
Abstract

BWMK1,a first reported rice MAPK gene,was transcriptionally activated by blast fungus infection and wounding.To make a further understanding about the BWMK1-mediated defense signaling,we identified 7 BWMK1 interacting genes by yeast two-hybrid screening,designate BWIP1~BWIP7,and selected BWIP4 for further functional characterization.Through Agrobacterium mediated transformation method,we generated overexpression and RNAi transgenic plant using a japonica cultivar nipponbare as a receptor.The GUS assay and PCR results showed that the target gene had been intergrated into receptor.Further expression analysis was confirmed by RT-PCR.These transgenic lines provided us a good material for in-deepth function analysis of BWIP4.

Published
2010

47. Polymorphism analysis of different rice varieties with SSR and SFP marks

Author

Huang HongMei, Xiao Yinghui, Liu Xionglun, Dai LiangYing, Huang Ling, and Liu Er-ming

Subjects
Genetics, Genetic marker, Polymorphism (computer science), Significant difference, food and beverages, Microsatellite, Polymorphism analysis, Chromosome 9, Biology, Genetic analysis, Tetranucleotide Repeats
Abstract

914 marks(848 SSR and 66 SFP)were used to find the genomic polymorphism between the blast resistant rice variety Xiangzi3150 and the blast susceptible variety CO39.The results showed that expected bands were amplified from 96.39% of 881 markers(815 SSR and 66 SFP),among which 220 polymorphic tags were identified and 24.10% were.The polymorphism rate of the SSR mark(23.58%) was lower than that of the SFP mark(30.30%).The rate of polymorphic marks on the 12 chromosomes was different.Chromosome 10 had the highest rate(30.00%) and chromosome 9 had the lowest rate(14.55%).SSR marks with different units also showed a significant difference in polymorphism rate.The SSR marks with tetranucleotide repeats had the highest polymorphism rate(27.96%),followed by the repetitive dinucleotide,repetitive trinucleotide and repetitive single-nucleotide tags,respectively.

Published
2010

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