Back to Search
Start Over
Transformation of the BWMK1-interacting gene BWIP4
- Source :
- JOURNAL OF HUNAN AGRICULTURAL UNIVERSITY. 36:272-275
- Publication Year :
- 2010
- Publisher :
- China Science Publishing & Media Ltd., 2010.
-
Abstract
- BWMK1,a first reported rice MAPK gene,was transcriptionally activated by blast fungus infection and wounding.To make a further understanding about the BWMK1-mediated defense signaling,we identified 7 BWMK1 interacting genes by yeast two-hybrid screening,designate BWIP1~BWIP7,and selected BWIP4 for further functional characterization.Through Agrobacterium mediated transformation method,we generated overexpression and RNAi transgenic plant using a japonica cultivar nipponbare as a receptor.The GUS assay and PCR results showed that the target gene had been intergrated into receptor.Further expression analysis was confirmed by RT-PCR.These transgenic lines provided us a good material for in-deepth function analysis of BWIP4.
Details
- ISSN :
- 10071032
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- JOURNAL OF HUNAN AGRICULTURAL UNIVERSITY
- Accession number :
- edsair.doi...........22fc01e25666326db0ee207c802f565b