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Guangcai Shao,1 Xiang Li,2 Meiyue Da,1 Xiaochuan Huo,3 Shuheng Zhang1 1Department of Neurosurgery, Anshan Central Hospital, Anshan, People’s Republic of China; 2Department of Neurosurgery, The First Affiliated Hospital of China Medical University, Shenyang, People’s Republic of China; 3Cerebrovascular Disease Department, Neurological Disease Center, Beijing Anzhen Hospital, Capital Medical University, Beijing, People’s Republic of ChinaCorrespondence: Shuheng Zhang, Department of Neurosurgery, Anshan Central Hospital, No. 77 South Zhonghua Road, Tiedong District, Anshan, 114009, People’s Republic of China, Tel +8618804120515, Email 1223443553@qq.com Xiaochuan Huo, Cerebrovascular Disease Department, Neurological Disease Center, Beijing Anzhen Hospital, Capital Medical University, Beijing, People’s Republic of China, Email huoxiaochuan@126.comBackground and Purpose: Studies on outcome comparison after endovascular treatment (EVT) for large vessel occlusion (LVO) between large artery atherosclerosis (LAA) and cardioembolism (CE) in the Asian population are scarce. We aimed to compare the baseline characteristics and clinical outcomes after EVT for anterior circulation LVO with LAA and CE in the Chinese population.Methods: Patients were selected from the ANGEL registry and divided into LAA and CE groups. The primary outcome was the 90-day modified Rankin Scale (mRS) 0– 2. The secondary outcomes were 90-day mRS distribution, 90-day mRS 0– 1, 90-day mRS 0– 3, and early neurological improvement. The safety outcomes included death, symptomatic intracranial hemorrhage, and any intracranial hemorrhage. We conducted logistic regression models with adjustments to compare the outcomes.Results: A total of 632 patients were included, of whom, 488 were in the LAA group and 144 were in the CE group. No significant difference in 90-day mRS 0– 2 was observed between LAA and CE groups (55.7%vs.43.1%, odds ratio[OR] 1.19, 95% confidence interval(CI), 0.92– 1.53, P=0.190). The LAA group exhibited a higher frequency of mRS 0– 3 compared to the CE group (69.1% vs 32.6%, OR1.32, 95% CI 1.02– 1.72, P=0.038). However, the incidence of death within 90 days did not significantly differ between the LAA and CE groups (10.9%vs.24.3%, OR0.91, 95% CI0.66– 1.25, P=0.545), nor did the occurrences of symptomatic intracranial hemorrhage(SICH) (4.5%vs.9.7%,OR1.08, 95% CI 0.65– 1.78, P=0.779) or intracranial hemorrhage(ICH) (21.9%vs.30.6%, OR 0.94, 95% CI0.71– 1.25, P=0.680). Moreover, no significant disparities were detected in other outcomes between the two groups (All P> 0.05).Conclusion: In the ANGEL registry, a higher prevalence of patients undergoing EVT for acute anterior circulation LVO with LAA was found than those with CE. However, our study revealed that the efficacy and safety of EVT remained consistent regardless of the stroke’s etiology such as LAA or CE.Keywords: large vessel occlusion, endovascular treatment, large artery atherosclerosis, cardioembolism

Xi Yang,1 Miao Da,2 Wenyuan Zhang,3 Quan Qi,4 Chun Zhang,5 Shuwen Han4 1Department of Intervention and Radiotherapy, Huzhou Central Hospital, 2Medical College of Nursing, Huzhou University, 3Department of Gynaecology, 4Department of Medical Oncology, 5Department of Infectious Diseases, Huzhou Central Hospital, Huzhou, Zhejiang Province, People’s Republic of China Abstract: Cervical cancer is a common malignant cancer among women worldwide. Changes in the vaginal microecological environment lead to multiple gynecological diseases, including cervical cancer. Recent research has shown that Lactobacillus may play an important role in the occurrence and development of cervical cancer. This review explores the role of Lactobacillus in cervical cancer. A total of 29 articles were included after identification and screening. The pertinent literature on Lactobacillus in cervical cancer from two perspectives, including clinical studies and experimental studies, was analyzed. An association network for the mechanism by which Lactobacillus induces cervical cancer was constructed. In addition, we provide direction and insight for further research on the role of Lactobacillus in cervical cancer. Keywords: CIN, cervical cancer, Lactobacillus, microorganism

The structural and dielectric properties of BaMoO4 - CaTiO3 advanced ceramics in the Radio Frequency (RF) region (< 300 MHz) were studied in the present work. X-ray diffraction and Rietveld refinement showed no evidence of secondary phases, indicating no reaction between the BaMoO4 (BMO) and CaTiO3 (CTO) phases. Complex Impedance Spectroscopy (CIS) analysis demonstrated that BMO presents greater permittivity than composites can be explained by the low densification of the composites. Nyquist diagrams were fitted employing an equivalent circuit with two associations of R-CPE (constant phase element) related to the grain and the grain boundary effects. The composites showed a decrease in the values of Temperature Capacitance Coefficient (TCC) with CTO (CaTiO3) content increase, whereas composites BMO20 and BMO40 showed TCC values equal to zero at 1 MHz. Moreover, as obtained by CIS analysis, the activation energy of thermo-activated processes decreases with CTO concentration increase. [ABSTRACT FROM AUTHOR]

Serological tests are critical tools in the fight against infectious disease. They detect antibodies produced during an adaptive immune response against a pathogen with an immunological reagent, whose antibody binding characteristics define the specificity and sensitivity of the assay. While pathogen proteins have conveniently served as reagents, their performance is limited by the natural grouping of specific and non-specific antibody binding sites, epitopes. An attractive solution is to build synthetic proteins that only contains pathogen-specific epitopes, which could theoretically reach 100% specificity. However, the genesis of de novo proteins remains a challenge. To address the uncertainty of producing a synthetic protein, we have repurposed the beta barrel of fluorescent proteins into a receptacle that can receive several epitope sequences without compromising its ability to be expressed. Here, two versions of a multiepitope protein were built using the receptacle that differ by their grouping of epitopes specific to the parasite Trypanosoma cruzi, the causative agent for Chagas disease. An evaluation of their performance as the capture reagent in ELISAs showed near-complete agreement with recommended diagnostic protocols. The results suggest that a single assay could be developed for the diagnosis of Chagas disease and that this approach could be applied to other diseases. [ABSTRACT FROM AUTHOR]

The present work shows the dielectric properties of (100-X)Ba2TiSi2O8–(X)TiO2 composites (X in wt%) at radio frequency (RF) region (< 300 MHz). X-ray diffraction and Rietveld's refinement showed the presence of Ba2(Ti7/9Si2/9)9O20 (BTSO) phase in the studied composites. To analyze the relationship between the electrical properties and the microstructure of the matrix and composites, Impedance spectroscopy (IS) was used. The applied model that best describes the behavior found in the samples is the Havriliak–Negami model. From the IS it was observed that the dielectric relative permittivity presented high values in BTS10 (X = 10%) and BTS20 (X = 20%) which allows classifying them as Colossal permittivity materials. This is an important result due to the potential for applications in new storage systems, capacitive devices and microelectronics. Nyquist diagrams were employed to evaluate the contribution of the grain and grain boundary in the electrical response of the samples and fitted employing an equivalent circuit with two associations of R-CPE (constant phase element). Temperature coefficient of capacitance (TCC) was also obtained and it was possible to observe that there was an increase in the TCC value for the compounds in all frequencies. In addition, IS demonstrated that the activation energy decreasing with TiO2 addition indicating a decrease in the resistive character of the materials. [ABSTRACT FROM AUTHOR]

Objective: This retrospective case series aimed to assess the stability of the papilla around four single crowns supported by narrow‐diameter implants replacing all maxillary incisors. Secondary objectives included assessment of marginal bone level stability, incidence of technical and biological complications, and patient satisfaction. Materials and Methods: Individuals with four adjacent implants in maxillary incisor sites, placed with a 3 mm inter‐implant distance and rehabilitated with single crowns were included. Retrospective data were obtained from photographs and radiographs taken at the delivery of the prosthesis (baseline—T0). Patients were then recalled (≥2 years after T0) for clinical and radiographic examination (follow‐up—T1). Photographs were obtained and patient satisfaction was assessed using a visual analogue scale. Papilla height and marginal bone level were compared over time. Results: Data from 10 patients with medium‐low smile lines and rehabilitated with 40 implants, in function for 5.4 ± 1.9 years, were analyzed. The papilla height between implants (T0: 2.3 ± 0.9 mm; T1: 2.6 ± 0.7 mm; p =.011) and between tooth and implant (T0: 3.4 ± 0.9 mm; T1: 3.8 ± 0.8 mm; p =.025) increased significantly over the years. The marginal bone level remained stable over time (T0: 0.88 ± 0.57 mm; T1: 0.71 ± 0.67 mm; p =.007). Patients were highly satisfied (97.7 ± 0.3%) with the treatment outcome. Conclusion: Within its limitations, this study demonstrated that four single implant‐supported crowns placed at maxillary incisor sites may exhibit soft tissue and marginal bone stability over a long period of time. This treatment approach, however, should be restricted to few patients as it requires a proper case selection and skillful execution of all surgical and prosthetic steps. [ABSTRACT FROM AUTHOR]

This work studies the dielectric properties in the microwave region (MW) of the Ba2TiSi2O8 (BTS) ceramic with TiO2 additions and its applications as a dielectric resonator antenna (DRA). In this study, structural characterization through x-ray diffraction (XRD) is performed and the Rietveld refinement is used to confirm the phases formed. Analysis of the morphology of the materials is performed using scanning electron microscopy (SEM). The resonant frequency temperature coefficient (τf) reveals a variation from − 47.0 ppm°C−1 to + 16.5 ppm°C−1. The dielectric properties in the MW region reveal an increase in the dielectric permittivity (εr) and a decrease in the loss tangent (tanδ) of the samples. Numerical simulation shows good fits of the experimental data, with gain and directivity standing out, ranging from 4 dBi to 6 dBi and radiation efficiency below 80%. The results demonstrate that the samples can operate in C-band electronics, Wi-Fi devices, meteorological radar systems, etc. [ABSTRACT FROM AUTHOR]

Copyright of Revista Caatinga is the property of Revista Caatinga and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Abstract Clinically, the body mass index remains the most frequently used metric of overall obesity, although it is flawed by its inability to account for different adipose (i.e., visceral, subcutaneous, and inter/intramuscular) compartments, as well as muscle mass. Numerous prior studies have demonstrated linkages between specific adipose or muscle compartments to outcomes of multiple diseases. Although there are no universally accepted standards for body composition measurement, many studies use a single slice at the L3 vertebral level. In this study, we use computed tomography (CT) studies from patients in The Cancer Genome Atlas (TCGA) to compare current L3-based techniques with volumetric techniques, demonstrating potential limitations with level-based approaches for assessing outcomes. In addition, we identify gene expression signatures in normal kidney that correlate with fat and muscle body composition traits that can be used to predict sex-specific outcomes in renal cell carcinoma.

This article evaluates a diagnostic algorithm for mastocytosis in skin biopsies and CD25 expression in skin mast cells of 49 patients at a Tertiary Hospital in Sao Paulo, Brazil. Mastocytosis is characterized by the accumulation of neoplastic mast cells in the skin or other organs. The study found that the algorithm was able to correctly diagnose all mastocytosis cases, but the significance of CD25 expression in skin mast cells is still unclear. Further research is needed to identify skin mast cell markers that can correlate with disease chronicity and severity. [Extracted from the article]

Copyright of HOLOS is the property of Instituto Federal do Rio Grande do norte - IFRN and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This research aimed to analyze the perception of tourism stakeholders regarding the potential of gastronomy to revitalize tourism in the Mirandela region. The sample consisted of 95 stakeholders (e.g., managers, team leaders, directors, entrepreneurs) operating in the tourism sector. The study was operationalized using a qualitative methodology, and the data were analyzed using MAXQDA software. The results demonstrated that food quality, identity gastronomy, and food heritage are considered differentiating factors of the local cuisine. Additionally, it was found that diversification of tourism offerings, variety of traditional dishes, and multiplier effect are considered essential elements to enhance local gastronomy and boost tourism in the region. It was possible to ascertain that the promotion of local gastronomy as a tourist product depends on the organization of gastronomic events, preservation of culture, investment in digital marketing, and immersive experiences. Finally, it was observed that among the main measures to position the region as a gastronomic tourist destination are support from the private sector, synergies among local stakeholders, investment in infrastructure, and integration of local gastronomy with other tourism activities.

Abstract Background Low anterior resection syndrome (LARS) is a distressing condition that affects approximately 25–80% of patients following surgery for rectal cancer. LARS is characterized by debilitating bowel dysfunction symptoms, including fecal incontinence, urgent bowel movements, and increased frequency of bowel movements. Although biofeedback therapy has demonstrated effectiveness in improving postoperative rectal control, the research results have not fulfilled expectations. Recent research has highlighted that stimulating the pudendal perineal nerves has a superior impact on enhancing pelvic floor muscle function than biofeedback alone. Hence, this study aims to evaluate the efficacy of a combined approach integrating biofeedback with percutaneous electrical pudendal nerve stimulation (B-PEPNS) in patients with LARS through a randomized controlled trial (RCT). Methods and analysis In this two-armed multicenter RCT, 242 participants with LARS after rectal surgery will be randomly assigned to undergo B-PEPNS (intervention group) or biofeedback (control group). Over 4 weeks, each participant will undergo 20 treatment sessions. The primary outcome will be the LARS score. The secondary outcomes will be anorectal manometry and pelvic floor muscle electromyography findings and the European Organization for the Research and Treatment of Cancer Quality of Life Questionnaire-Colorectal 29 (EORTC QLQ-CR29) scores. Data will be collected at baseline, post-intervention (1 month), and follow-up (6 months). Discussion We anticipate that this study will contribute further evidence regarding the efficacy of B-PEPNS in alleviating LARS symptoms and enhancing the quality of life for patients following rectal cancer surgery. Trial registration Chinese Clincal Trials Register ChiCTR2300078101. Registered 28 November 2023.

Objetivo: Descrever um caso de satelitismo plaquetário como causa de pseudotrombocitopenia detectado na rotina de um grande laboratório. Introdução: O satelitismo plaquetário é um fenômeno raro que ocorre somente in vitro e pode ser visto à hematoscopia de indivíduos saudáveis ou com outras patologias, inclusive hematológicas. Caracteriza-se por trombocitopenia espúria ao hemograma completo e pela disposição de grande número de plaquetas em torno de neutrófilos na lâmina de esfregaço de sangue periférico preparados a partir da amostra colhida em tubo com Ácido EtilenoDiamonoTetra-Acético de sódio (EDTA). A formação dessas rosetas plaquetárias pode ser ainda observada ocasionalmente em torno de outras células como eosinófilos, basófilos, linfócitos ou monócitos. Embora o mecanismo que leva a essa alteração não seja plenamente compreendido, acredita-se que o EDTA possa agir diretamente modificando proteínas de membrana das plaquetas e dos leucócitos, resultando em pontes de interação entre ambos. Há, ainda, a possibilidade de ação indireta promovida pelo EDTA expondo criptoantígenos plaquetários que poderiam então interagir com autoanticorpos contra epítopos de plaquetas e neutrófilos simultaneamente. Relato de caso: Em novembro de 2023, deu entrada em nossa área técnica do Rio de Janeiro amostra colhida em tubo EDTA para a realização de hemograma completo de paciente do sexo masculino, 25 anos, sem comorbidades. O analisador XN-Sysmex liberouo resultado com contagem de plaquetas de 98.000/mm3, com sinalização de presença de grumos. Seguindo a rotina de nosso laboratório, amostra foi encaminhada para distensão automatizada e análise no equipamento CellaVision. À análise da hematoscopia, observamos numerosos neutrófilos com grande quantidade de plaquetas ao redor de seu citoplasma com aspecto de rosetas, configurando o satelitismo plaquetário como causa provável da trombocitopenia espúria. Como não dispúnhamos de amostra coletada em anticoagulante diferente (citrato ou CPDA) e não tínhamos acesso direto ao cliente para realizarmos distensão direta a partir de digito-punção, optamos por submeter a amostra ao agitador de alta frequência (Digital Vortex – ThermoScientific), com a correção da contagem plaquetária para a faixa da normalidade de 223.000/mm3. Discussão: A trombocitopenia é alteração frequentemente encontrada nos ambulatórios de Hematologia. As etiologias vão desde autoimunes, passando por doenças metabólicas / carenciais até falências medulares. No entanto, é fundamental a exclusão das trombocitopenias espúrias, principalmente em reduções leves a moderadas das contagens. Isso evita investigações desnecessárias, procedimentos invasivos, custos em saúde e estresse psicológico nos pacientes. Apesar do satelitismo ser evento raro, a pseudotrombocitopenia é relativamente frequente e deve estar sempre no leque de diagnósticos diferenciais. Conclusão: Seja em indivíduos saudáveis ou em pacientes portadores de doenças hematológicas ou autoimunes, a trombocitopenia espúria pode levar à investigação desnecessária ou, em casos mais graves, a procedimentos mal indicados. Por essa razão, a análise da hematoscopia por técnicos experientes e hematologistas deve sempre ser realizada como o primeiro passo na investigação de trombocitopenias detectadas ao hemograma completo.

Objetivo: Analisar in silico as atividades biológicas de compostos químicos da própolis com potencial anticancerígenas. Metodologia: Através da plataformas PubChem foi realizado a busca dos compostos químicos específicos da própolis e direcionados ao Pass Online, para obter dados sobre atividades biológicas e farmacológicas. Os compostos selecionados e coletados pelo SMILES canônicos (estrutura de uma molécula) foram classificados de acordo com PA (Probabilidade de atividade) e PI (Probabilidade de inatividade), com PA > 0,7 sendo os compostos: 7-Hidroxiflavanona, Pinocembrina, Pinostrobina e Pinobanksin. Resultado: Efeitos dos compostos de ser bioativas: 7-Hidroxiflavanona:Membrane integrity agonist (PA = 0,955 e PI = 0,003) e Antimutagenic (PA = 0,809 e PI = 0,004); Pinocembrina: Membrane integrity agonist (PA = 0,962 e PI = 0,003), Antimutagenic(PA = 0,842 e PI = 0,003), TP53 expression enhancer (PA = 0,816 e PI = 0,009), Antioxidant (PA = 0,793 e PI = 0,003), Apoptosis agonist (PA = 0,752 e PI = 0,011), Antineoplastic (PA = 0,746 e PI = 0,019) e Anticarcinogenic (PA = 0,721 e PI = 0,008); Pinostrobina: Membrane integrity agonist (PA = 0,953 e PI = 0,003), Antimutagenic (PA = 0,817 e PI = 0,004), TP53 expression enhancer (PA = 0,799 e PI = 0,011), Apoptosis agonist (PA = 0,730 e PI = 0,012), Antineoplastic (PA = 0,730 e PI = 0,021 e Anticarcinogenic (PA = 0,705 e PI = 0,008); Pinobanksin: Membrane integrity agonist (PA = 0,973 e PI = 0,002), TP53 expression enhancer (PA = 0,962 e PI = 0,003), Antioxidant (PA = 0,940 e PI = 0,002), Antimutagenic (PA = 0,883e PI = 0,002), Anticarcinogenic (PA = 0,780 e PI = 0,006), Apoptosis agonist (PA = 0,756 e PI = 0,010), Antiinflammatory (PA = 0,712 e PI = 0,014) e Antineoplastic (PA = 0,706 e PI = 0,025). Discussão: No estudo conduzido por Moghadari Masoud et al. (2022), foi explorado o potencial antitumoral da própolis de Kerman como tratamento das células NALM-6 com concentrações variáveis de própolis de Kerman e doxorrubicina durante 72 horas. Os resultados demonstraram que tanto a própolis de Kerman quanto a doxorrubicina induziram a morte celular por apoptose de forma dose-dependente, com uma regulação significativa da razão Bax/Bcl-2, sugerindo um efeito pró-apoptótico. O que endossa os estudo dirigido por Franchi Jr. et al. (2011), em que investigaram o efeito dos extratos etanólicos de própolis grupo 12 (G12) e própolis grupo 13 (G13) em células de leucemia humana, em que os valores de IC50 para os extratos de própolis vermelha (G13) e verde (G12) variaram significativamente entre diferentes linhagens de células de leucemia. A própolis vermelha demonstrou ser mais potente em termos citotóxicos, especialmente nas células K562 e HL60, e apresentou menor dispersão nos valores de IC50. Além disso, ao comparar o efeito da própolis vermelha com os fármacos clínicos Gleevec e citarabina, foi identificada uma diferença marcante nas células K562 e Nalm16, com a G12 mostrando um efeito citotóxico mais significativo. Conclusão: A composição química da própolis pode ser útil para linhagem de leucemia linfoblástica aguda (NALM-6), o que envolve principalmente compostos que possuem atividades anticancerígenas. Esses compostos podem interferir no ciclo celular, induzir apoptose e reduzir o estresse oxidativo nas células cancerígenas com potencial para afetar as células NALM-6.