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The publication of the seventh edition of “Revista Ambiente e Agua – An Interdisciplinary Journal of Applied Science (Ambi-Agua)” demonstrates the international visibility that the journal is reaching. An unprecedented number of submissions has been received for this issue and resulted in an increased number (50%) of published articles, as compared to the first two volumes that had six articles published. This issue brings nine peer reviewed articles out of which 5 are published in English. Among those two were submitted from abroad (Nigeria and Argentina) in addition to several paper co-authors from the United States. The participation in the authorship of public universities and municipal, state and federal institutions was also significant. There were 18 authors in this issue from those institutions not considering those authors from abroad. There was also a thematic coverage expansion of articles within the interdisciplinary scope of Ambi-Agua. An example of this is an article in Engineering concerned with thermal comfort in urban areas; several submissions in environmental chemistry with two published articles (organic and inorganic chemistry); one article in the area of public health and antimicrobial resistance in aquatic ecosystem; other in sensibility to oil spilling charting in coastal islands; chemical contamination in fish due to the improper land use in Amazonia; and two articles in hydrological modeling in watersheds. Powered by the OAI-PMH protocol (Batista, 2007), which greatly enhances the visibility of the journal, in addition to the fact that Ambi-Agua appears in several international indexing systems and its free on-line availability with open access policy, the journal is being frequently consulted. In only two months (June 15 to August 15, 2008) there were 4,556 consultations with an average of approximately 500 consultations a week. These consultations came from 616 cities, 79 countries, and five continents in that period.

The main objective of this study was to evaluate the presence of Ochratoxin A (OTA) in wheat grains and wheat flour samples using a new high performance liquid chromatography (HPLC) method. The experiment used six wheat grain samples from different industry storage place from Chapeco (SC), South Brazil, on August 2008. The OTA extraction was carried out using acetonitrile:water (120:80 v/v) as solvent. Thereafter, the supernatant was filtered, and applied on OTA-specific immunoafinity column to HPLC. Furthermore, the co¬lumn was washedAwith water and the toxin was eluted with methanol. The OTA wheat grains and wheat flour concentration were analyzed by a fluorescence detector coupled to the HPLC apparatus. The results showed a smaller OTA concentration than the limits set by inter¬national legislation. El objetivo principal de este estudio fue evaluar la presencia de Ocratoxina-A (OTA) en los granos del trigo y harina del trigo realizadas por un nuevo método de determinación que usa la cromatografía líquida de alta resolución (CLAR) acoplada al descubridor del fluorimetrio. El experimento usó seis muestras de grano de trigo del lugar del almacenamiento diferente a la industria local de Chapeco (SC), Brasil Sur, en agosto, 2008. El extracto de OTA era llevado a cabo usando el acetonitrila:agua (120:80 v/v) como solventes. Después el suprenadante fue filtrado, y aplicado en la columna del inmunoafinidad específica a OTA. Además, la columna se lavó con agua y la toxina era el eluido con el metanol. La determinación del OTA se realizó por detección de fluorescencia acoplado al aparato de HPLC. Los volúmenes de OTA en los granos del trigo y harina del trigo eran entonces los determínate y los resultados mostraron una concentración de OTA menor que los límites exigidos por la legislación internacional.