32 results on '"Crowet JM"'
Search Results
2. Challenging level of rigid-body approach involving numerical elements (CHLORAINE) applied to repeated elastin peptides.
- Author
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Depenveiller C, Wong H, Crowet JM, Debelle L, Baud S, Dauchez M, and Belloy N
- Subjects
- Amino Acid Sequence, Molecular Dynamics Simulation, Biocompatible Materials, Elastin, Peptides chemistry
- Abstract
Elastic proteins and derived biomaterials contain numerous tandemly repeated peptides along their sequences, ranging from a few copies to hundreds. These repetitions are responsible for their biochemical, biological and biomechanical properties. These sequences are considered to be intrinsically disordered, and the variations in their behavior are actually mainly due to their high flexibility and lack of stable secondary structures originating from their unique amino acid sequences. Consequently, the simulation of elastic proteins and large elastomeric biomaterials using classical molecular dynamics is an important challenge. Here, we propose a novel approach that allows the application of the DURABIN protocol to repeated elastin-like peptides (r-ELPs) in a simple way. Four large r-ELPs were studied to evaluate our method, which was developed for simulating extracellular matrix proteins at the mesoscopic scale. After structure clustering applied on molecular dynamic trajectories of constitutive peptides (5-mers and 6-mers), the main conformations were used as starting points to define the corresponding primitives, further used as rigid body fragments in our program. Contributions derived from electrostatic and molecular hydrophobicity potentials were tested to evaluate their influence on the interactions during simple mesoscopic simulations. The CHLORAINE approach, despite the thinner granularity due to the size of the patterns used, was included in the DURABIN protocol and emerges as a promising way to simulate elastic macromolecular systems., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Inc. All rights reserved.)
- Published
- 2023
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3. Modelling and Simulations of Extracellular Glycoproteins.
- Author
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Rao RM, Belloy N, Crowet JM, Dauchez M, and Baud S
- Subjects
- Glycosylation, Models, Molecular, Glycoproteins chemistry, Protein Processing, Post-Translational
- Abstract
While the knowledge of protein structure and function has seen vast advances in previous decades, the understanding of how their posttranslational modifications, such as glycosylations, influence their structure and function remains poor. However, advances in in silico methodologies to study glycosylations in recent past have enabled us to study this and understand the role of glycosylations in protein structure and function in ways that would not be possible by conventional experimental methods. In this chapter, we will demonstrate how to leverage these methodologies to study glycoproteins and their structural and dynamic properties using molecular modelling techniques., (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
- Published
- 2023
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4. Multiscale modelling of the extracellular matrix.
- Author
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Wong H, Crowet JM, Dauchez M, Ricard-Blum S, Baud S, and Belloy N
- Abstract
The extracellular matrix is a complex three-dimensional network of molecules that provides cells with a complex microenvironment. The major constituents of the extracellular matrix such as collagen, elastin and associated proteins form supramolecular assemblies contributing to its physicochemical properties and organization. The structure of proteins and their supramolecular assemblies such as fibrils have been studied at the atomic level (e.g., by X-ray crystallography, Nuclear Magnetic Resonance and cryo-Electron Microscopy) or at the microscopic scale. However, many protein complexes are too large to be studied at the atomic level and too small to be studied by microscopy. Most extracellular matrix components fall into this intermediate scale, so-called the mesoscopic scale, preventing their detailed characterization. Simulation and modelling are some of the few powerful and promising approaches that can deepen our understanding of mesoscale systems. We have developed a set of modelling tools to study the self-organization of the extracellular matrix and large motion of macromolecules at the mesoscale level by taking advantage of the dynamics of articulated rigid bodies as a mean to study a larger range of motions at the cost of atomic resolution., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2021 The Author(s).)
- Published
- 2021
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5. Sitosterol and glucosylceramide cooperative transversal and lateral uneven distribution in plant membranes.
- Author
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Rondelli V, Koutsioubas A, Pršić J, Deboever E, Crowet JM, Lins L, and Deleu M
- Subjects
- Cell Membrane metabolism, Glucosylceramides metabolism, Lipid Bilayers metabolism, Plant Cells metabolism, Sitosterols metabolism
- Abstract
The properties of biomembranes depend on the presence, local structure and relative distribution assumed by the thousands of components it is made of. As for animal cells, plant membranes have been demonstrated to be organized in subdomains with different persistence lengths and times. In plant cells, sitosterol has been demonstrated to confer to phospholipid membranes a more ordered structure while among lipids, glycosphingolipids are claimed to form rafts where they tightly pack with sterols. Glucosylceramides are glycosphingolipids involved in plant signalling and are essential for viability of cells and whole plant. The glucosylceramide-sitosterol structural coupling within PLPC membranes is here investigated by Langmuir films, in silico simulations and neutron reflectometry, unveiling that a strong direct interaction between the two molecules exists and governs their lateral and transversal distribution within membrane leaflets. The understanding of the driving forces governing specific molecules clustering and segregation in subdomains, such as glucosylceramide and sitosterol, have an impact on the mechanical properties of biomembranes and could reflect in the other membrane molecules partitioning and activity., (© 2021. The Author(s).)
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- 2021
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6. LIMONADA: A database dedicated to the simulation of biological membranes.
- Author
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Crowet JM, Buchoux S, Belloy N, Sarazin C, Lins L, and Dauchez M
- Subjects
- Databases, Factual, Cell Membrane chemistry, Lipids chemistry, Molecular Dynamics Simulation
- Abstract
Cellular membranes are composed of a wide diversity of lipid species in varying proportions and these compositions are representative of the organism, cellular type and organelle to which they belong. Because models of these molecular systems simulated by MD steadily gain in size and complexity, they are increasingly representative of specific compositions and behaviors of biological membranes. Due to the number of lipid species involved, of force fields and topologies and because of the complexity of membrane objects that have been simulated, LIMONADA has been developed as an open database allowing to handle the various aspects of lipid membrane simulation. LIMONADA presents published membrane patches with their simulation files and the cellular membrane it models. Their compositions are then detailed based on the lipid identification from LIPID MAPS database plus the lipid topologies and the force field used. LIMONADA is freely accessible on the web at https://limonada.univ-reims.fr/., (© 2021 Wiley Periodicals LLC.)
- Published
- 2021
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7. Blending Ferulic Acid Derivatives and Polylactic Acid into Biobased and Transparent Elastomeric Materials with Shape Memory Properties.
- Author
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Gallos A, Crowet JM, Michely L, Raghuwanshi VS, Mention MM, Langlois V, Dauchez M, Garnier G, and Allais F
- Subjects
- Coumaric Acids, Polyesters, Polymers
- Abstract
Thanks to its remarkable properties such as sustainability, compostability, biocompatibility, and transparency, poly-l-lactic acid (PLA) would be a suitable replacement for oil-based polymers should it not suffer from low flexibility and poor toughness, restricting its use to rigid plastic by excluding elastomeric applications. Indeed, there are few fully biobased and biodegradable transparent elastomers-PLA-based or not-currently available. In the last decades, many strategies have been investigated to soften PLA and enhance its toughness and elongation at break by using plasticizers, oligomers, or polymers. This work shows how a ferulic acid-derived biobased additive (BDF) blends with a common rigid and brittle commercial grade of polylactic acid to provide a transparent non-covalently cross-linked elastomeric material with shape memory behavior exhibiting an elongation at break of 434% (vs 6% for pristine PLA). Through a structure-activity relationship analysis conducted with BDF analogues and a modeling study, we propose a mechanism based on π-π stacking to account for the elastomeric properties. Blending ferulic acid derivatives with polylactic acid generates a new family of fully sustainable transparent elastomeric materials with functional properties such as shape memory.
- Published
- 2021
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8. Correction: Structural basis for plant plasma membrane protein dynamics and organization into functional nanodomains.
- Author
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Gronnier J, Crowet JM, Habenstein B, Nasir MN, Bayle V, Hosy E, Platre MP, Gouguet PB, Raffaele S, Martinez D, Grelard A, Loquet A, Simon-Plas F, Gerbeau-Pissot P, Der C, Bayer EM, Jaillais Y, Deleu M, Germain V, Lins L, and Mongrand S
- Published
- 2021
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9. The Trypanosoma Brucei KIFC1 Kinesin Ensures the Fast Antibody Clearance Required for Parasite Infectivity.
- Author
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Lecordier L, Uzureau S, Vanwalleghem G, Deleu M, Crowet JM, Barry P, Moran B, Voorheis P, Dumitru AC, Yamaryo-Botté Y, Dieu M, Tebabi P, Vanhollebeke B, Lins L, Botté CY, Alsteens D, Dufrêne Y, Pérez-Morga D, Nolan DP, and Pays E
- Abstract
Human innate immunity to Trypanosoma brucei involves the trypanosome C-terminal kinesin Tb KIFC1, which transports internalized trypanolytic factor apolipoprotein L1 (APOL1) within the parasite. We show that Tb KIFC1 preferentially associates with cholesterol-containing membranes and is indispensable for mammalian infectivity. Knockdown of Tb KIFC1 did not affect trypanosome growth in vitro but rendered the parasites unable to infect mice unless antibody synthesis was compromised. Surface clearance of Variant Surface Glycoprotein (VSG)-antibody complexes was far slower in these cells, which were more susceptible to capture by macrophages. This phenotype was not due to defects in VSG expression or trafficking but to decreased VSG mobility in a less fluid, stiffer surface membrane. This change can be attributed to increased cholesterol level in the surface membrane in Tb KIFC1 knockdown cells. Clearance of surface-bound antibodies by T. brucei is therefore essential for infectivity and depends on high membrane fluidity maintained by the cholesterol-trafficking activity of Tb KIFC1., Competing Interests: The authors declare that they have no competing financial interest., (© 2020 The Author(s).)
- Published
- 2020
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10. Structural Analysis of Nonapeptides Derived from Elastin.
- Author
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Hernández B, Crowet JM, Thiery J, Kruglik SG, Belloy N, Baud S, Dauchez M, and Debelle L
- Subjects
- Circular Dichroism, Extracellular Matrix, Humans, Elastin, Peptides
- Abstract
Elastin-derived peptides are released from the extracellular matrix remodeling by numerous proteases and seem to regulate many biological processes, notably cancer progression. The canonical elastin peptide is VGVAPG, which harbors the XGXXPG consensus pattern, allowing interaction with the elastin receptor complex located at the surface of cells. Besides these elastokines, another class of peptides has been identified. This group of bioactive elastin peptides presents the XGXPGXGXG consensus sequence, but the reason for their bioactivity remains unexplained. To better understand their nature and structure-function relationships, herein we searched the current databases for this nonapeptide motif and observed that the XGXPGXGXG elastin peptides define a specific group of tandemly repeated patterns. Further, we focused on four tandemly repeated human elastin nonapeptides, i.e., AGIPGLGVG, VGVPGLGVG, AGVPGLGVG, and AGVPGFGAG. These peptides were analyzed by means of optical spectroscopies and molecular dynamics. Ultraviolet-circular dichroism and Raman spectra are consistent with a mixture of β-turn, β-strand, and random-chain secondary elements in aqueous media. Quantitative analysis of their conformations suggested that turns corresponded to half of the total population of structural elements, whereas the remaining half were equally distributed between β-strand and unordered chains. These distributions were confirmed by molecular dynamics simulations. Altogether, our data suggest that these highly dynamic peptides harbor a type II β-turn located in their central part. We hypothesize that this structural element could explain their specific bioactivity., (Copyright © 2020 Biophysical Society. Published by Elsevier Inc. All rights reserved.)
- Published
- 2020
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11. Molecular Model for the Self-Assembly of the Cyclic Lipodepsipeptide Pseudodesmin A.
- Author
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Crowet JM, Sinnaeve D, Fehér K, Laurin Y, Deleu M, Martins JC, and Lins L
- Subjects
- Acetonitriles chemistry, Chloroform chemistry, Magnetic Resonance Spectroscopy, Models, Chemical, Models, Molecular, Peptides, Cyclic chemistry, Protein Conformation, Solvents, Molecular Dynamics Simulation, Peptides, Cyclic chemical synthesis
- Abstract
Self-assembly of peptides into supramolecular structures represents an active field of research with potential applications ranging from material science to medicine. Their study typically involves the application of a large toolbox of spectroscopic and imaging techniques. However, quite often, the structural aspects remain underexposed. Besides, molecular modeling of the self-assembly process is usually difficult to handle, since a vast conformational space has to be sampled. Here, we have used an approach that combines short molecular dynamics simulations for peptide dimerization and NMR restraints to build a model of the supramolecular structure from the dimeric units. Experimental NMR data notably provide crucial information about the conformation of the monomeric units, the supramolecular assembly dimensions, and the orientation of the individual peptides within the assembly. This in silico / in vitro mixed approach enables us to define accurate atomistic models of supramolecular structures of the bacterial cyclic lipodepsipeptide pseudodesmin A.
- Published
- 2019
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12. Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata.
- Author
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Brault ML, Petit JD, Immel F, Nicolas WJ, Glavier M, Brocard L, Gaston A, Fouché M, Hawkins TJ, Crowet JM, Grison MS, Germain V, Rocher M, Kraner M, Alva V, Claverol S, Paterlini A, Helariutta Y, Deleu M, Lins L, Tilsner J, and Bayer EM
- Subjects
- Arabidopsis cytology, Arabidopsis growth & development, Cell Membrane metabolism, Cells, Cultured, Endoplasmic Reticulum metabolism, Gene Expression Profiling, Gene Expression Regulation, Developmental, Genes, Reporter, Glycosyltransferases deficiency, Green Fluorescent Proteins genetics, Green Fluorescent Proteins metabolism, Humans, Luminescent Proteins genetics, Luminescent Proteins metabolism, Membrane Proteins deficiency, Phospholipids metabolism, Plant Cells, Plants, Genetically Modified, Plasmodesmata metabolism, Plasmodesmata ultrastructure, Protein Domains, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae metabolism, Signal Transduction, Nicotiana genetics, Nicotiana metabolism, Red Fluorescent Protein, Arabidopsis genetics, Arabidopsis Proteins genetics, Gene Expression Regulation, Plant, Glycosyltransferases genetics, Membrane Proteins genetics, Plasmodesmata genetics
- Abstract
In eukaryotes, membrane contact sites (MCS) allow direct communication between organelles. Plants have evolved a unique type of MCS, inside intercellular pores, the plasmodesmata, where endoplasmic reticulum (ER)-plasma membrane (PM) contacts coincide with regulation of cell-to-cell signalling. The molecular mechanism and function of membrane tethering within plasmodesmata remain unknown. Here, we show that the multiple C2 domains and transmembrane region protein (MCTP) family, key regulators of cell-to-cell signalling in plants, act as ER-PM tethers specifically at plasmodesmata. We report that MCTPs are plasmodesmata proteins that insert into the ER via their transmembrane region while their C2 domains dock to the PM through interaction with anionic phospholipids. A Atmctp3/Atmctp4 loss of function mutant induces plant developmental defects, impaired plasmodesmata function and composition, while MCTP4 expression in a yeast Δtether mutant partially restores ER-PM tethering. Our data suggest that MCTPs are unique membrane tethers controlling both ER-PM contacts and cell-to-cell signalling., (© 2019 The Authors. Published under the terms of the CC BY 4.0 license.)
- Published
- 2019
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13. Low-diluted Phenacetinum disrupted the melanoma cancer cell migration.
- Author
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Fuselier C, Terryn C, Berquand A, Crowet JM, Bonnomet A, Molinari M, Dauchez M, Martiny L, and Schneider C
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- Animals, Biomechanical Phenomena drug effects, Cell Line, Tumor, Cell Membrane drug effects, Cell Membrane metabolism, Cell Polarity drug effects, Dose-Response Relationship, Drug, Mice, Melanoma, Cutaneous Malignant, Cell Movement drug effects, Melanoma pathology, Phenacetin pharmacology, Skin Neoplasms pathology
- Abstract
Dynamic and reciprocal interactions generated by the communication between tumor cells and their matrix microenvironment, play a major role in the progression of a tumor. Indeed, the adhesion of specific sites to matrix components, associated with the repeated and coordinated formation of membrane protrusions, allow tumor cells to move along a determined pathway. Our study analyzed the mechanism of action of low-diluted Phenacetinum on murine cutaneous melanoma process in a fibronectin matrix environment. We demonstrated a reduction of dispersed cell migration, early and for as long as 24 h, by altering the formation of cell protrusions. Moreover, low-diluted Phenacetinum decreased cell stiffness highly on peripheral areas, due to a disruption of actin filaments located just under the plasma membrane. Finally, it modified the structure of the plasma membrane by accumulating large ordered lipid domains and disrupted B16 cell migration by a likely shift in the balance between ordered and disordered lipid phases. Whereas the correlation between the excess of lipid raft and cytoskeleton disrupting is not as yet established, it is clear that low-diluted Phenacetinum acts on the actin cytoskeleton organization, as confirmed by a decrease of cell stiffness affecting ultimately the establishment of an effective migration process.
- Published
- 2019
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14. Linoleic and linolenic acid hydroperoxides interact differentially with biomimetic plant membranes in a lipid specific manner.
- Author
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Deleu M, Deboever E, Nasir MN, Crowet JM, Dauchez M, Ongena M, Jijakli H, Fauconnier ML, and Lins L
- Subjects
- Biomimetics, Cell Membrane metabolism, Linolenic Acids metabolism, Lipid Peroxides metabolism, Plants metabolism
- Abstract
Linoleic and linolenic acid hydroperoxides (HPOs) constitute key intermediate oxylipins playing an important role as signaling molecules during plant defense processes in response to biotic or abiotic stress. They have also been demonstrated in vitro as antimicrobial agents against plant fungi and bacteria. To reach the phytopathogens in vivo, the HPOs biosynthesized in the plant cells must cross the plant plasma membrane (PPM) where they can also interact with plasma membrane lipids and have an effect on their organization. In the present study, we have investigated the interaction properties of HPOs with PPM at a molecular level using biophysical tools combining in vitro and in silico approaches and using plant biomimetic lipid systems. Our results have shown that HPOs are able to interact with PPM lipids and perturb their lateral organization. Glucosylceramide (GluCer) is a privileged partner, sitosterol lessens their binding and the presence of both GluCer and sitosterol further reduces their interaction. Hydrophobic effect and polar interactions are involved in the binding. The chemical structure of HPOs influences their affinity for PPM lipids. The presence of three double bonds in the HPO molecule gives rise to a higher affinity comparatively to two double bonds, which can be explained by their differential interaction with the lipid polar headgroups., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2019
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15. Homology modeling and in vivo functional characterization of the zinc permeation pathway in a heavy metal P-type ATPase.
- Author
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Lekeux G, Crowet JM, Nouet C, Joris M, Jadoul A, Bosman B, Carnol M, Motte P, Lins L, Galleni M, and Hanikenne M
- Subjects
- Adenosine Triphosphatases metabolism, Arabidopsis enzymology, Arabidopsis metabolism, Arabidopsis Proteins metabolism, Biological Transport, Models, Genetic, Structural Homology, Protein, Adenosine Triphosphatases genetics, Arabidopsis genetics, Arabidopsis Proteins genetics, Zinc metabolism
- Abstract
The P1B ATPase heavy metal ATPase 4 (HMA4) is responsible for zinc and cadmium translocation from roots to shoots in Arabidopsis thaliana. It couples ATP hydrolysis to cytosolic domain movements, enabling metal transport across the membrane. The detailed mechanism of metal permeation by HMA4 through the membrane remains elusive. Here, homology modeling of the HMA4 transmembrane region was conducted based on the crystal structure of a ZntA bacterial homolog. The analysis highlighted amino acids forming a metal permeation pathway, whose importance was subsequently investigated functionally through mutagenesis and complementation experiments in plants. Although the zinc pathway displayed overall conservation among the two proteins, significant differences were observed, especially in the entrance area with altered electronegativity and the presence of a ionic interaction/hydrogen bond network. The analysis also newly identified amino acids whose mutation results in total or partial loss of the protein function. In addition, comparison of zinc and cadmium accumulation in shoots of A. thaliana complemented lines revealed a number of HMA4 mutants exhibiting different abilities in zinc and cadmium translocation. These observations could be instrumental to design low cadmium-accumulating crops, hence decreasing human cadmium exposure.
- Published
- 2019
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16. Insight into the Self-Assembling Properties of Peptergents: A Molecular Dynamics Simulation Study.
- Author
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Crowet JM, Nasir MN, Dony N, Deschamps A, Stroobant V, Morsomme P, Deleu M, Soumillion P, and Lins L
- Subjects
- Amino Acids chemistry, Detergents pharmacology, Membrane Proteins chemistry, Peptides pharmacology, Protein Conformation, Structure-Activity Relationship, Water chemistry, Detergents chemistry, Molecular Dynamics Simulation, Peptides chemistry
- Abstract
By manipulating the various physicochemical properties of amino acids, the design of peptides with specific self-assembling properties has been emerging for more than a decade. In this context, short peptides possessing detergent properties (so-called "peptergents") have been developed to self-assemble into well-ordered nanostructures that can stabilize membrane proteins for crystallization. In this study, the peptide with "peptergency" properties, called ADA8 and extensively described by Tao et al., is studied by molecular dynamic simulations for its self-assembling properties in different conditions. In water, it spontaneously forms beta sheets with a β barrel-like structure. We next simulated the interaction of this peptide with a membrane protein, the bacteriorhodopsin, in the presence or absence of a micelle of dodecylphosphocholine. According to the literature, the peptergent ADA8 is thought to generate a belt of β structures around the hydrophobic helical domain that could help stabilize purified membrane proteins. Molecular dynamic simulations are here used to image this mechanism and provide further molecular details for the replacement of detergent molecules around the protein. In addition, we generalized this behavior by designing an amphipathic peptide with beta propensity, which was called ABZ12. Both peptides are able to surround the membrane protein and displace surfactant molecules. To our best knowledge, this is the first molecular mechanism proposed for "peptergency".
- Published
- 2018
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17. Interaction between the barley allelochemical compounds gramine and hordenine and artificial lipid bilayers mimicking the plant plasma membrane.
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Lebecque S, Crowet JM, Lins L, Delory BM, du Jardin P, Fauconnier ML, and Deleu M
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- Hydrogen Bonding, Indole Alkaloids chemistry, Indole Alkaloids toxicity, Liposomes, Molecular Dynamics Simulation, Phase Transition, Pheromones chemistry, Pheromones toxicity, Phosphatidylcholines chemistry, Toxicity Tests, Transition Temperature, Tyramine chemistry, Tyramine metabolism, Tyramine toxicity, Cell Membrane metabolism, Hordeum metabolism, Indole Alkaloids metabolism, Lipid Bilayers metabolism, Pheromones metabolism, Tyramine analogs & derivatives
- Abstract
Some plants affect the development of neighbouring plants by releasing secondary metabolites into their environment. This phenomenon is known as allelopathy and is a potential tool for weed management within the framework of sustainable agriculture. While many studies have investigated the mode of action of various allelochemicals (molecules emitted by allelopathic plants), little attention has been paid to their initial contact with the plant plasma membrane (PPM). In this paper, this key step is explored for two alkaloids, gramine and hordenine, that are allelochemicals from barley. Using in vitro bioassays, we first showed that gramine has a greater toxicity than hordenine towards a weed commonly found in northern countries (Matricaria recutita L.). Then, isothermal titration calorimetry was used to show that these alkaloids spontaneously interact with lipid bilayers that mimic the PPM. The greater impact of gramine on the thermotropic behaviour of lipids compared to hordenine was established by means of infrared spectroscopy. Finally, the molecular mechanisms of these interactions were explored with molecular dynamics simulations. The good correlation between phytotoxicity and the ability to disturb lipid bilayers is discussed. In this study, biophysical tools were used for the first time to investigate the interactions of allelochemicals with artificial PPM.
- Published
- 2018
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18. Differential Interaction of Synthetic Glycolipids with Biomimetic Plasma Membrane Lipids Correlates with the Plant Biological Response.
- Author
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Nasir MN, Lins L, Crowet JM, Ongena M, Dorey S, Dhondt-Cordelier S, Clément C, Bouquillon S, Haudrechy A, Sarazin C, Fauconnier ML, Nott K, and Deleu M
- Subjects
- Biomimetics, Cell Membrane, Lipid Bilayers, Membrane Lipids, Glycolipids chemistry
- Abstract
Natural and synthetic amphiphilic molecules including lipopeptides, lipopolysaccharides, and glycolipids are able to induce defense mechanisms in plants. In the present work, the perception of two synthetic C14 rhamnolipids, namely, Alk-RL and Ac-RL, differing only at the level of the lipid tail terminal group have been investigated using biological and biophysical approaches. We showed that Alk-RL induces a stronger early signaling response in tobacco cell suspensions than does Ac-RL. The interactions of both synthetic RLs with simplified biomimetic membranes were further analyzed using experimental and in silico approaches. Our results indicate that the interactions of Alk-RL and Ac-RL with lipids were different in terms of insertion and molecular responses and were dependent on the lipid composition of model membranes. A more favorable insertion of Alk-RL than Ac-RL into lipid membranes is observed. Alk-RL forms more stable molecular assemblies than Ac-RL with phospholipids and sterols. At the molecular level, the presence of sterols tends to increase the RLs' interaction with lipid bilayers, with a fluidizing effect on the alkyl chains. Taken together, our findings suggest that the perception of these synthetic RLs at the membrane level could be related to a lipid-driven process depending on the organization of the membrane and the orientation of the RLs within the membrane and is correlated with the induction of early signaling responses in tobacco cells.
- Published
- 2017
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19. Structural basis for plant plasma membrane protein dynamics and organization into functional nanodomains.
- Author
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Gronnier J, Crowet JM, Habenstein B, Nasir MN, Bayle V, Hosy E, Platre MP, Gouguet P, Raffaele S, Martinez D, Grelard A, Loquet A, Simon-Plas F, Gerbeau-Pissot P, Der C, Bayer EM, Jaillais Y, Deleu M, Germain V, Lins L, and Mongrand S
- Subjects
- Biophysical Phenomena, Microscopy, Cell Membrane chemistry, Plant Proteins analysis, Nicotiana chemistry, Nicotiana physiology
- Abstract
Plasma Membrane is the primary structure for adjusting to ever changing conditions. PM sub-compartmentalization in domains is thought to orchestrate signaling. Yet, mechanisms governing membrane organization are mostly uncharacterized. The plant-specific REMORINs are proteins regulating hormonal crosstalk and host invasion. REMs are the best-characterized nanodomain markers via an uncharacterized moiety called REMORIN C-terminal Anchor. By coupling biophysical methods, super-resolution microscopy and physiology, we decipher an original mechanism regulating the dynamic and organization of nanodomains. We showed that targeting of REMORIN is independent of the COP-II-dependent secretory pathway and mediated by PI4P and sterol. REM-CA is an unconventional lipid-binding motif that confers nanodomain organization. Analyses of REM-CA mutants by single particle tracking demonstrate that mobility and supramolecular organization are critical for immunity. This study provides a unique mechanistic insight into how the tight control of spatial segregation is critical in the definition of PM domain necessary to support biological function.
- Published
- 2017
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20. Membrane Interactions of Natural Cyclic Lipodepsipeptides of the Viscosin Group.
- Author
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Geudens N, Nasir MN, Crowet JM, Raaijmakers JM, Fehér K, Coenye T, Martins JC, Lins L, Sinnaeve D, and Deleu M
- Subjects
- Bacterial Proteins chemistry, Bacterial Proteins metabolism, Circular Dichroism, Gram-Negative Bacteria drug effects, Gram-Positive Bacteria drug effects, Lipid Bilayers metabolism, Lipopeptides metabolism, Lipopeptides pharmacology, Magnetic Resonance Spectroscopy, Microbial Sensitivity Tests, Peptides, Cyclic metabolism, Peptides, Cyclic pharmacology, Permeability drug effects, Pseudomonas metabolism, Spectroscopy, Fourier Transform Infrared, Lipid Bilayers chemistry, Lipopeptides chemistry, Peptides, Cyclic chemistry
- Abstract
Many Pseudomonas spp. produce cyclic lipodepsipeptides (CLPs), which, besides their role in biological functions such as motility, biofilm formation and interspecies interactions, are antimicrobial. It has been established that interaction with the cellular membrane is central to the mode of action of CLPs. In this work, we focus on the CLPs of the so-called viscosin group, aiming to assess the impact of the main structural variations observed within this group on both the antimicrobial activity and the interaction with model membranes. The antimicrobial activity of viscosin, viscosinamide A, WLIP and pseudodesmin A were all tested on a broad panel of mainly Gram-positive bacteria. Their capacity to permeabilize or fuse PG/PE/cardiolipin model membrane vesicles is assessed using fluorescent probes. We find that the Glu2/Gln2 structural variation within the viscosin group is the main factor that influences both the membrane permeabilization properties and the minimum inhibitory concentration of bacterial growth, while the configuration of the Leu5 residue has no apparent effect. The CLP-membrane interactions were further evaluated using CD and FT-IR spectroscopy on model membranes consisting of PG/PE/cardiolipin or POPC with or without cholesterol. In contrast to previous studies, we observe no conformational change upon membrane insertion. The CLPs interact both with the polar heads and aliphatic tails of model membrane systems, altering bilayer fluidity, while cholesterol reduces CLP insertion depth., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2017
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21. Molecular dynamics study of micelles properties according to their size.
- Author
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Lebecque S, Crowet JM, Nasir MN, Deleu M, and Lins L
- Subjects
- Hydrophobic and Hydrophilic Interactions, Sodium Dodecyl Sulfate chemistry, Surface Properties, Surface-Active Agents chemistry, Water chemistry, Micelles, Molecular Dynamics Simulation, Particle Size
- Abstract
Surfactants are molecules able to spontaneously self-assemble to form aggregates with well-defined properties, such as spherical micelles, planar bilayers, cylindrical micelles or vesicles. Micelles have notably several applications in many domains, such as drug delivery or membrane protein solubilization. In this context, the study of micelle formation in relation with the structural and physico-chemical properties of surfactants is of great interest to better control their use in the different application fields. In this work, we use the MD approach developed by Yoshii et al. and extend it to surfactants with different structures. We aim to systematically investigate different micellar properties as a function of the aggregates size by a molecular dynamics approach, to get an insight into the micellar organization and to collect some relevant descriptors about micelle formation. For this, we perform short MD simulations of preformed micelles of various sizes and analyze three parameters for each micelle size, namely the eccentricity of the micelles, the hydrophobic/hydrophilic surface ratio and the hydrophobic tails hydration. If these parameters are known descriptors of micelles, they were not yet studied in this way by MD. We show that eccentricity, used as "validator" parameter, exhibits minimal values when the aggregate size is close to the experimental aggregation number for surfactants that are known to form spherical micelles. This hence indicates that our methodology gives consistent results. The evolution of the two descriptors follows another scheme, with a sharp increase and decrease, respectively, followed by a leveling-off. The aggregate sizes at which this stabilization starts to occur are close to the respective aggregation number of each surfactant. In our approach, we validate the use of these descriptors to follow micelle formation by MD, from "simple" surfactants to more complex structures, like lipopeptides. Our calculations also suggest that some peculiar behavior, like that of TPC, can be highlighted by our approach. In the context of peptidic surfactants, our methodology could further help to improve computer simulations combined to molecular thermodynamic models to predict micellar properties of those more complex amphiphilic molecules., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2017
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22. Interactions of sugar-based bolaamphiphiles with biomimetic systems of plasma membranes.
- Author
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Nasir MN, Crowet JM, Lins L, Obounou Akong F, Haudrechy A, Bouquillon S, and Deleu M
- Subjects
- Adsorption, Binding Sites, Binding, Competitive, Biocompatible Materials chemical synthesis, Biocompatible Materials chemistry, Biocompatible Materials metabolism, Biomimetics, Cell Membrane metabolism, Furans chemical synthesis, Furans metabolism, Glycolipids chemistry, Glycolipids metabolism, Hydrophobic and Hydrophilic Interactions, Lipid Bilayers chemistry, Lipid Bilayers metabolism, Models, Molecular, Pyridones chemical synthesis, Pyridones metabolism, Rhamnose metabolism, Spectroscopy, Fourier Transform Infrared, Sterols chemistry, Sterols metabolism, Xylose metabolism, Cell Membrane chemistry, Furans chemistry, Pyridones chemistry, Rhamnose chemistry, Xylose chemistry
- Abstract
Glycolipids constitute a class of molecules with various biological activities. Among them, sugar-based bolaamphiphiles characterized by their biocompatibility, biodegradability and lower toxicity, became interesting for the development of efficient and low cost lipid-based drug delivery systems. Their activity seems to be closely related to their interactions with the lipid components of the plasma membrane of target cells. Despite many works devoted to the chemical synthesis and characterization of sugar-based bolaamphiphiles, their interactions with plasma membrane have not been completely elucidated. In this work, two sugar-based bolaamphiphiles differing only at the level of their sugar residues were chemically synthetized. Their interactions with membranes have been investigated using model membranes containing or not sterol and with in silico approaches. Our findings indicate that the nature of sugar residues has no significant influence for their membrane interacting properties, while the presence of sterol attenuates the interactions of both bolaamphiphiles with the membrane systems. The understanding of this distinct behavior of bolaamphiphiles towards sterol-containing membrane systems could be useful for their applications as drug delivery systems., (Copyright © 2016. Published by Elsevier B.V.)
- Published
- 2016
- Full Text
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23. Complementary biophysical tools to investigate lipid specificity in the interaction between bioactive molecules and the plasma membrane: A review.
- Author
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Deleu M, Crowet JM, Nasir MN, and Lins L
- Abstract
Plasma membranes are complex entities common to all living cells. The basic principle of their organization appears very simple, but they are actually of high complexity and represent very dynamic structures. The interactions between bioactive molecules and lipids are important for numerous processes, from drug bioavailability to viral fusion. The cell membrane is a carefully balanced environment and any change inflicted upon its structure by a bioactive molecule must be considered in conjunction with the overall effect that this may have on the function and integrity of the membrane. Conceptually, understanding the molecular mechanisms by which bioactive molecules interact with cell membranes is of fundamental importance. Lipid specificity is a key factor for the detailed understanding of the penetration and/or activity of lipid-interacting molecules and of mechanisms of some diseases. Further investigation in that way should improve drug discovery and development of membrane-active molecules in many domains such as health, plant protection or microbiology. In this review, we will present complementary biophysical approaches that can give information about lipid specificity at a molecular point of view. Examples of application will be given for different molecule types, from biomolecules to pharmacological drugs. A special emphasis is given to cyclic lipopeptides since they are interesting molecules in the scope of this review by combining a peptidic moiety and a lipidic tail and by exerting their activity via specific interactions with the plasma membrane., (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Published
- 2014
- Full Text
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24. Mechanism of Trypanosoma brucei gambiense resistance to human serum.
- Author
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Uzureau P, Uzureau S, Lecordier L, Fontaine F, Tebabi P, Homblé F, Grélard A, Zhendre V, Nolan DP, Lins L, Crowet JM, Pays A, Felu C, Poelvoorde P, Vanhollebeke B, Moestrup SK, Lyngsø J, Pedersen JS, Mottram JC, Dufourc EJ, Pérez-Morga D, and Pays E
- Subjects
- Africa, Animals, Animals, Genetically Modified, Apolipoprotein L1, Apolipoproteins antagonists & inhibitors, Apolipoproteins toxicity, Cell Membrane chemistry, Cell Membrane metabolism, Cysteine Proteases metabolism, Haptoglobins metabolism, Hemoglobins metabolism, Hemolysis, Humans, Hydrophobic and Hydrophilic Interactions, Lipid Metabolism, Lipoproteins, HDL antagonists & inhibitors, Lipoproteins, HDL chemistry, Lipoproteins, HDL toxicity, Parasites pathogenicity, Parasites physiology, Protein Structure, Secondary, Serum chemistry, Serum parasitology, Trypanosoma brucei gambiense drug effects, Trypanosoma brucei gambiense pathogenicity, Trypanosomiasis, African parasitology, Variant Surface Glycoproteins, Trypanosoma chemistry, Variant Surface Glycoproteins, Trypanosoma metabolism, Apolipoproteins blood, Apolipoproteins metabolism, Lipoproteins, HDL blood, Lipoproteins, HDL metabolism, Trypanosoma brucei gambiense physiology
- Abstract
The African parasite Trypanosoma brucei gambiense accounts for 97% of human sleeping sickness cases. T. b. gambiense resists the specific human innate immunity acting against several other tsetse-fly-transmitted trypanosome species such as T. b. brucei, the causative agent of nagana disease in cattle. Human immunity to some African trypanosomes is due to two serum complexes designated trypanolytic factors (TLF-1 and -2), which both contain haptoglobin-related protein (HPR) and apolipoprotein LI (APOL1). Whereas HPR association with haemoglobin (Hb) allows TLF-1 binding and uptake via the trypanosome receptor TbHpHbR (ref. 5), TLF-2 enters trypanosomes independently of TbHpHbR (refs 4, 5). APOL1 kills trypanosomes after insertion into endosomal/lysosomal membranes. Here we report that T. b. gambiense resists TLFs via a hydrophobic β-sheet of the T. b. gambiense-specific glycoprotein (TgsGP), which prevents APOL1 toxicity and induces stiffening of membranes upon interaction with lipids. Two additional features contribute to resistance to TLFs: reduction of sensitivity to APOL1 requiring cysteine protease activity, and TbHpHbR inactivation due to a L210S substitution. According to such a multifactorial defence mechanism, transgenic expression of T. b. brucei TbHpHbR in T. b. gambiense did not cause parasite lysis in normal human serum. However, these transgenic parasites were killed in hypohaptoglobinaemic serum, after high TLF-1 uptake in the absence of haptoglobin (Hp) that competes for Hb and receptor binding. TbHpHbR inactivation preventing high APOL1 loading in hypohaptoglobinaemic serum may have evolved because of the overlapping endemic area of T. b. gambiense infection and malaria, the main cause of haemolysis-induced hypohaptoglobinaemia in western and central Africa.
- Published
- 2013
- Full Text
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25. SAHBNET, an accessible surface-based elastic network: an application to membrane protein.
- Author
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Dony N, Crowet JM, Joris B, Brasseur R, and Lins L
- Subjects
- Bacterial Outer Membrane Proteins chemistry, Calibration, Computer Simulation, DNA-Directed RNA Polymerases chemistry, Escherichia coli Proteins chemistry, Hydrogen Bonding, Microfilament Proteins chemistry, Models, Molecular, Penicillin-Binding Proteins chemistry, Peptidoglycan Glycosyltransferase chemistry, Protein Structure, Tertiary, Rhinovirus enzymology, Serine-Type D-Ala-D-Ala Carboxypeptidase chemistry, Solubility, Solvents, Surface Properties, Water chemistry, Elasticity, Membrane Proteins chemistry
- Abstract
Molecular Dynamics is a method of choice for membrane simulations and the rising of coarse-grained forcefields has opened the way to longer simulations with reduced calculations times. Here, we present an elastic network, SAHBNET (Surface Accessibility Hydrogen-Bonds elastic NETwork), that will maintain the structure of soluble or membrane proteins based on the hydrogen bonds present in the atomistic structure and the proximity between buried residues. This network is applied on the coarse-grained beads defined by the MARTINI model, and was designed to be more physics-based than a simple elastic network. The SAHBNET model is evaluated against atomistic simulations, and compared with ELNEDYN models. The SAHBNET is then used to simulate two membrane proteins inserted in complex lipid bilayers. These bilayers are formed by self-assembly and the use of a modified version of the GROMACS tool genbox (which is accessible through the gcgs.gembloux.ulg.ac.be website). The results show that SAHBNET keeps the structure close to the atomistic one and is successfully used for the simulation of membrane proteins.
- Published
- 2013
- Full Text
- View/download PDF
26. Modeling of non-covalent complexes of the cell-penetrating peptide CADY and its siRNA cargo.
- Author
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Crowet JM, Lins L, Deshayes S, Divita G, Morris M, Brasseur R, and Thomas A
- Subjects
- Amino Acid Motifs, Arginine chemistry, Genetic Vectors, Hydrophobic and Hydrophilic Interactions, Models, Molecular, Molecular Conformation, Molecular Dynamics Simulation, Protein Binding, Protein Structure, Secondary, Static Electricity, Thermodynamics, Time Factors, Cell-Penetrating Peptides chemistry, Peptides chemistry, RNA, Small Interfering metabolism
- Abstract
CADY is a cell-penetrating peptide spontaneously making non-covalent complexes with Short interfering RNAs (siRNAs) in water. Neither the structure of CADY nor that of the complexes is resolved. We have calculated and analyzed 3D models of CADY and of the non-covalent CADY-siRNA complexes in order to understand their formation and stabilization. Data from the ab initio calculations and molecular dynamics support that, in agreement with the experimental data, CADY is a polymorphic peptide partly helical. Taking into consideration the polymorphism of CADY, we calculated and compared several complexes with peptide/siRNA ratios of up to 40. Four complexes were run by using molecular dynamics. The initial binding of CADYs is essentially due to the electrostatic interactions of the arginines with siRNA phosphates. Due to a repetitive arginine motif (XLWR(K)) in CADY and to the numerous phosphate moieties in the siRNA, CADYs can adopt multiple positions at the siRNA surface leading to numerous possibilities of complexes. Nevertheless, several complex properties are common: an average of 14±1 CADYs is required to saturate a siRNA as compared to the 12±2 CADYs experimentally described. The 40 CADYs/siRNA that is the optimal ratio for vector stability always corresponds to two layers of CADYs per siRNA. When siRNA is covered by the first layer of CADYs, the peptides still bind despite the electrostatic repulsion. The peptide cage is stabilized by hydrophobic CADY-CADY contacts thanks to CADY polymorphism. The analysis demonstrates that the hydrophobicity, the presence of several positive charges and the disorder of CADY are mandatory to make stable the CADY-siRNA complexes., (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Published
- 2013
- Full Text
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27. Multi-scale simulation of the simian immunodeficiency virus fusion peptide.
- Author
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Crowet JM, Parton DL, Hall BA, Steinhauer S, Brasseur R, Lins L, and Sansom MS
- Subjects
- Lipid Bilayers chemistry, Models, Biological, Molecular Dynamics Simulation, Peptides chemistry, Simian Immunodeficiency Virus chemistry, Viral Fusion Proteins chemistry
- Abstract
Fusion peptides of type I fusion glycoproteins are structural elements of several enveloped viruses which enable the fusion between host and virus membranes. It is generally suggested that these peptides can promote the early fusion steps by inducing membrane curvature and that they adopt a tilted helical conformation in membranes. Although this property has been the subject of several experimental and in silico studies, an extensive sampling of the membrane peptide interaction has not yet been done. In this study, we performed coarse-grained molecular dynamic simulations in which the lipid bilayer self-assembles around the peptide. The simulations indicate that the SIV fusion peptide can adopt two different orientations in a DPPC bilayer, a major population which adopts a tilted interfacial orientation and a minor population which is perpendicular to the bilayer. The simulations also indicate that for the SIV mutant that does not induce fusion in vitro the tilt is abolished.
- Published
- 2012
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28. Plasma membrane localization of Solanum tuberosum remorin from group 1, homolog 3 is mediated by conformational changes in a novel C-terminal anchor and required for the restriction of potato virus X movement].
- Author
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Perraki A, Cacas JL, Crowet JM, Lins L, Castroviejo M, German-Retana S, Mongrand S, and Raffaele S
- Subjects
- Agrobacterium tumefaciens genetics, Agrobacterium tumefaciens metabolism, Carrier Proteins genetics, Cell Membrane genetics, Cell Membrane virology, Circular Dichroism, Cloning, Molecular, Hydrophobic and Hydrophilic Interactions, Lipid Bilayers metabolism, Membrane Microdomains genetics, Membrane Microdomains metabolism, Membrane Proteins genetics, Membrane Proteins metabolism, Models, Biological, Mutation, Phosphoproteins genetics, Plant Diseases virology, Plant Leaves genetics, Plant Leaves metabolism, Plant Leaves virology, Plant Proteins genetics, Potexvirus pathogenicity, Protein Binding, Protein Folding, Protein Structure, Secondary, Protein Transport, Solanum tuberosum genetics, Solanum tuberosum virology, Structure-Activity Relationship, Carrier Proteins metabolism, Cell Membrane metabolism, Phosphoproteins metabolism, Plant Proteins metabolism, Potexvirus metabolism, Solanum tuberosum metabolism
- Abstract
The formation of plasma membrane (PM) microdomains plays a crucial role in the regulation of membrane signaling and trafficking. Remorins are a plant-specific family of proteins organized in six phylogenetic groups, and Remorins of group 1 are among the few plant proteins known to specifically associate with membrane rafts. As such, they are valuable to understand the molecular bases for PM lateral organization in plants. However, little is known about the structural determinants underlying the specific association of group 1 Remorins with membrane rafts. We used a structure-function approach to identify a short C-terminal anchor (RemCA) indispensable and sufficient for tight direct binding of potato (Solanum tuberosum) REMORIN 1.3 (StREM1.3) to the PM. RemCA switches from unordered to α-helical structure in a nonpolar environment. Protein structure modeling indicates that RemCA folds into a tight hairpin of amphipathic helices. Consistently, mutations reducing RemCA amphipathy abolished StREM1.3 PM localization. Furthermore, RemCA directly binds to biological membranes in vitro, shows higher affinity for Detergent-Insoluble Membranes lipids, and targets yellow fluorescent protein to Detergent-Insoluble Membranes in vivo. Mutations in RemCA resulting in cytoplasmic StREM1.3 localization abolish StREM1.3 function in restricting potato virus X movement. The mechanisms described here provide new insights on the control and function of lateral segregation of plant PM.
- Published
- 2012
- Full Text
- View/download PDF
29. Impacts of the carbonyl group location of ester bond on interfacial properties of sugar-based surfactants: experimental and computational evidences.
- Author
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Razafindralambo H, Blecker C, Mezdour S, Deroanne C, Crowet JM, Brasseur R, Lins L, and Paquot M
- Subjects
- Air, Carbohydrate Conformation, Computer Simulation, Glucose chemistry, Glucuronates chemistry, Molecular Weight, Rheology, Surface Tension, Time Factors, Viscoelastic Substances chemistry, Water chemistry, Carbohydrates chemistry, Esters chemistry, Models, Molecular, Surface-Active Agents chemistry
- Abstract
Interfacial properties of surfactants are dependent on the conformation adopted by the hydrophilic headgroup or/and the hydrophobic tail at the boundary limit of two immiscible phases. Here, we demonstrate the impacts of the carbonyl group (-CO-) location of the ester bond of sugar-based surfactants by comparing some properties of two closely related esters, octyl glucuronate and glucose octanoate, at the air-water interface. The carbonyl group location influences the rate and extent of interfacial adsorption and the rheology properties of sugar esters at the air-water interface, which were evaluated by dynamic surface tension and complex surface viscoelastic measurements. Octyl glucuronate adsorbs the fastest at the air-water interface whereas glucose octanoate reduces the dynamic surface tension at the lowest value and exhibits the highest film viscoelasticity. Differences are attributed to molecular conformation constraints inducing relevant changes to the surface coverage kinetic capacity and the interaction strengths of the octyl sugar ester adsorbed films at the air-water interface. All of the results are supported by the minimum cross-sectional area values per molecule determined by both experimental and computational approaches.
- Published
- 2009
- Full Text
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30. Study of Thermomyces lanuginosa lipase in the presence of tributyrylglycerol and water.
- Author
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Santini S, Crowet JM, Thomas A, Paquot M, Vandenbol M, Thonart P, Wathelet JP, Blecker C, Lognay G, Brasseur R, Lins L, and Charloteaux B
- Subjects
- Amino Acid Sequence, Binding Sites, Catalytic Domain, Hydrogen Bonding, Models, Molecular, Molecular Sequence Data, Protein Structure, Tertiary, Sequence Alignment, Ascomycota enzymology, Fungal Proteins chemistry, Lipase chemistry, Triglycerides chemistry, Water chemistry
- Abstract
The Thermomyces lanuginosa lipase has been extensively studied in industrial and biotechnological research because of its potential for triacylglycerol transformation. This protein is known to catalyze both hydrolysis at high water contents and transesterification in quasi-anhydrous conditions. Here, we investigated the Thermomyces lanuginosa lipase structure in solution in the presence of a tributyrin aggregate using 30 ns molecular-dynamics simulations. The water content of the active-site groove was modified between the runs to focus on the protein-water molecule interactions and their implications for protein structure and protein-lipid interactions. The simulations confirmed the high plasticity of the lid fragment and showed that lipid molecules also bind to a secondary pocket beside the lid. Together, these results strongly suggest that the lid plays a role in the anchoring of the protein to the aggregate. The simulations also revealed the existence of a polar channel that connects the active-site groove to the outside solvent. At the inner extremity of this channel, a tyrosine makes hydrogen bonds with residues interacting with the catalytic triad. This system could function as a pipe (polar channel) controlled by a valve (the tyrosine) that could regulate the water content of the active site.
- Published
- 2009
- Full Text
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31. Tilted properties of the 67-78 fragment of alpha-synuclein are responsible for membrane destabilization and neurotoxicity.
- Author
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Crowet JM, Lins L, Dupiereux I, Elmoualija B, Lorin A, Charloteaux B, Stroobant V, Heinen E, and Brasseur R
- Subjects
- Circular Dichroism, Humans, Lewy Bodies pathology, Models, Molecular, Neurotoxins toxicity, Parkinson Disease pathology, Phospholipids, Protein Conformation, Stress, Mechanical, alpha-Synuclein analysis, Peptide Fragments toxicity, alpha-Synuclein chemistry
- Abstract
Alpha-synuclein is a 140 residue protein associated with Parkinson's disease. Intraneural inclusions called Lewy bodies and Lewy neurites are mainly composed of alpha-synuclein aggregated into amyloid fibrils. Other amyloidogenic proteins, such as the beta amyloid peptide involved in Alzheimer's disease and the prion protein (PrP) associated with Creuztfeldt-Jakob's disease, are known to possess "tilted peptides". These peptides are short protein fragments that adopt an oblique orientation at a hydrophobic/hydrophilic interface, which enables destabilization of the membranes. In this paper, sequence analysis and molecular modelling predict that the 67-78 fragment of alpha-synuclein is a tilted peptide. Its destabilizing properties were tested experimentally. The alpha-synuclein 67-78 peptide is able to induce lipid mixing and leakage of unilamellar liposomes. The neuronal toxicity, studied using human neuroblastoma cells, demonstrated that the alpha-synuclein 67-78 peptide induces neurotoxicity. A mutant designed by molecular modelling to be amphipathic was shown to be significantly less fusogenic and toxic than the wild type. In conclusion, we have identified a tilted peptide in alpha-synuclein, which could be involved in the toxicity induced during amyloidogenesis of alpha-synuclein., (2007 Wiley-Liss, Inc.)
- Published
- 2007
- Full Text
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32. The N-terminal 12 residue long peptide of HIV gp41 is the minimal peptide sufficient to induce significant T-cell-like membrane destabilization in vitro.
- Author
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Charloteaux B, Lorin A, Crowet JM, Stroobant V, Lins L, Thomas A, and Brasseur R
- Subjects
- Algorithms, Amino Acid Motifs, Cell Membrane, Cell Membrane Permeability, Computer Simulation, HIV Envelope Protein gp41 physiology, HIV-1 physiology, Liposomes chemistry, Peptides physiology, T-Lymphocytes physiology, HIV Envelope Protein gp41 chemistry, HIV-1 chemistry, Membrane Lipids chemistry, Models, Molecular, T-Lymphocytes chemistry
- Abstract
Here, we predicted the minimal N-terminal fragment of gp41 required to induce significant membrane destabilization using IMPALA. This algorithm is dedicated to predict peptide interaction with a membrane. We based our prediction of the minimal fusion peptide on the tilted peptide theory. This theory proposes that some protein fragments having a peculiar distribution of hydrophobicity adopt a tilted orientation at a hydrophobic/hydrophilic interface. As a result of this orientation, tilted peptides should disrupt the interface. We analysed in silico the membrane-interacting properties of gp41 N-terminal peptides of different length derived from the isolate BRU and from an alignment of 710 HIV strains available on the Los Alamos National Laboratory. Molecular modelling results indicated that the 12 residue long peptide should be the minimal fusion peptide. We then assayed lipid-mixing and leakage of T-cell-like liposomes with N-terminal peptides of different length as first challenge of our predictions. Experimental results confirmed that the 12 residue long peptide is necessary and sufficient to induce membrane destabilization to the same extent as the 23 residue long fusion peptide. In silico analysis of some fusion-incompetent mutants presented in the literature further revealed that they cannot insert into a modelled membrane correctly tilted. According to this work, the tilted peptide model appears to explain at least partly the membrane destabilization properties of HIV fusion peptide.
- Published
- 2006
- Full Text
- View/download PDF
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