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Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata.
- Source :
-
EMBO reports [EMBO Rep] 2019 Aug; Vol. 20 (8), pp. e47182. Date of Electronic Publication: 2019 Jul 09. - Publication Year :
- 2019
-
Abstract
- In eukaryotes, membrane contact sites (MCS) allow direct communication between organelles. Plants have evolved a unique type of MCS, inside intercellular pores, the plasmodesmata, where endoplasmic reticulum (ER)-plasma membrane (PM) contacts coincide with regulation of cell-to-cell signalling. The molecular mechanism and function of membrane tethering within plasmodesmata remain unknown. Here, we show that the multiple C2 domains and transmembrane region protein (MCTP) family, key regulators of cell-to-cell signalling in plants, act as ER-PM tethers specifically at plasmodesmata. We report that MCTPs are plasmodesmata proteins that insert into the ER via their transmembrane region while their C2 domains dock to the PM through interaction with anionic phospholipids. A Atmctp3/Atmctp4 loss of function mutant induces plant developmental defects, impaired plasmodesmata function and composition, while MCTP4 expression in a yeast Δtether mutant partially restores ER-PM tethering. Our data suggest that MCTPs are unique membrane tethers controlling both ER-PM contacts and cell-to-cell signalling.<br /> (© 2019 The Authors. Published under the terms of the CC BY 4.0 license.)
- Subjects :
- Arabidopsis cytology
Arabidopsis growth & development
Cell Membrane metabolism
Cells, Cultured
Endoplasmic Reticulum metabolism
Gene Expression Profiling
Gene Expression Regulation, Developmental
Genes, Reporter
Glycosyltransferases deficiency
Green Fluorescent Proteins genetics
Green Fluorescent Proteins metabolism
Humans
Luminescent Proteins genetics
Luminescent Proteins metabolism
Membrane Proteins deficiency
Phospholipids metabolism
Plant Cells
Plants, Genetically Modified
Plasmodesmata metabolism
Plasmodesmata ultrastructure
Protein Domains
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae metabolism
Signal Transduction
Nicotiana genetics
Nicotiana metabolism
Red Fluorescent Protein
Arabidopsis genetics
Arabidopsis Proteins genetics
Gene Expression Regulation, Plant
Glycosyltransferases genetics
Membrane Proteins genetics
Plasmodesmata genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1469-3178
- Volume :
- 20
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- EMBO reports
- Publication Type :
- Academic Journal
- Accession number :
- 31286648
- Full Text :
- https://doi.org/10.15252/embr.201847182