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Background: A nutritional supplement based on medicinal plants (saffron and ashwagandha), tryptophan, and vitamin B6 could contribute to alleviating/improving mood and associated disorders. The aim of this study was to evaluate the potential benefits of this combination supplement. During the study period, participants underwent a period of forced home confinement due to the COVID‐19 pandemic, which represented an unexpected impact factor. Methods: This open‐label prospective trial enrolled a cohort of female employees who reported mild to moderate anxiety. The primary objective was to evaluate changes in the level of anxiety using the adapted Hamilton Anxiety Rating Scale (HARS) after 12 weeks of regular supplementation with Safromotive (two tablets daily, for 12 weeks). The secondary objectives were to evaluate health‐related quality of life (HRQoL) and tolerability. Results: In total, 46 women with a mean age of 45.0 (6.5) years were included. A statistically significant improvement in HARS was observed, with a 7.5‐unit decrease from baseline to 12 weeks (p < 0.0001) and from 4 to 12 weeks of supplement intake (p = 0.0058). However, no significant changes were found during the lockdown period (between weeks 8 and 12 of the study). No relationship was found between women's sociodemographic characteristics and the HARS total score. A significant reduction in the HRQoL questionnaire score of 1.2 units was observed between baselines and 12 weeks of treatment (p = 0.0273). At the end of the study, 78.6% of the women reported consistency the supplement intake during the study course. Conclusion: This nutritional supplement composed of saffron, ashwagandha, tryptophan, and vitamin B6 appears to improve anxiety and HRQoL, but confinement could have impacted the evolution of the outcome. [ABSTRACT FROM AUTHOR]

Palladium (Pd) and zinc oxide (ZnO) (Pd/ZnO NPs) bimettalic nanocomposites still lag much too far behind other nanoparticles investigated for various biological uses in the area of cancer treatments. Chemically created nanoparticles agglomerate under physiological conditions, impeding their use in biomedical applications. In this study, a straightforward and environmentally friendly method for creating bimetallic nanoparticles (NPs) by combining palladium (Pd) and zinc oxide (ZnO) using Crocus sativus extract (CS-Pd/ZnO NCs) was reported; the bio-synthesize bimetallic palladium/zinc oxide nanocomposites and their antioxidant and anti-cancer properties were assessed. The developed Pd/ZnO NPs were characterized using different approaches, including UV-vis, DLS, FTIR, EDX, and SEM analyses. The present investigation shows how nanocomposites are made, their distinctive properties, antioxidant activity, anticancer mechanisms, and their potential therapeutic applications. DPPH and ABTS tests were used to investigate antioxidant activity. Further, the effects of CS-Pd/ZnO NCs on HeLa cells were assessed using the cell viability, ROS generation, MMP levels, and induced apoptosis. Apoptosis induction was measured using an Annexin V-fluorescein isothicyanate assay. Cell DNA was stained with propidium iodide to evaluate the impact upon this cell cycle. Time-dependent cell death was carried on by CS-Pd/ZnO NCs. The maximum inhibitory effect was 59 ± 3.2 when dosages of 4.5 µg/mL or higher were delivered after 24 h of treatment. Additionally, the CS-Pd/ZnO NCs caused HeLa cells to undergo apoptosis. Apoptotic HeLa cells were present in 35.64% of the treated cells at 4.5 µg/mL, and the cell cycle arrest at G0/G1 phase occurred concurrently. According to these findings, the CS-Pd/ZnO NCs may be a promising candidate for the creation of brand-new cervical cancer treatment. [ABSTRACT FROM AUTHOR]

Background: Animal and human studies have demonstrated that the saffron and the active components of saffron, including crocin, crocetin, and safranal, possess anti-inflammatory, antioxidant, and immunomodulatory properties. In this meta-analysis, the preclinical evidence and potential mechanism of saffron were explored in an animal model of ovalbumin-induced asthma. Methods: Studies related to saffron and its constituents in an animal model of ovalbumin-induced asthma from the beginning to March 2024 were searched from Scopus, PubMed, andWeb of Science databases. The methodological quality of the studieswas evaluated using the 15-item CAMARADES checklist. Data analysis was performed using STATA software version 17. Results: Thirteen studies with 536 animals (268 animals in the intervention group and 268 animals in the ovalbumin-induced group) were analyzed. The meta-analysis findings demonstrated that saffron and its constituents played a significant role in reducing total WBC, eosinophil, lymphocyte, and monocyte counts. Moreover, saffron showed a significant decrease in the levels of IL-4, IL-5, IL-13, IgE, histamine, endothelin, nitric oxide, and nitrite. Moreover, saffron was found to elevate EC50 thresholds and lower maximum response rates in experimental animals. The analysis revealed a significant identification of modulation in endoplasmic reticulum (ER) stress markers and miRNAs pathways. Conclusion: Saffron and its components may impact ovalbumin-induced asthma model in animals through anti-inflammatory, antioxidant, and immunomodulatory pathways, as well as improving pulmonary function and modulating ER stress markers and miRNAs pathways. As a result, saffron should be considered for further clinical trials in individuals suffering from asthma. [ABSTRACT FROM AUTHOR]

Saffron "Crocus sativus" is a plant of the Iridaceae family. Its therapeutic virtues have been known since antiquity; it is used in traditional medicine and culinary preparations. It is also known for its use in cosmetics because of its beneficial pharmacological activities for human skin. In particular, saffron tepals are the main by-product of saffron processing; they contain several bioactive compounds such as mineral agents, anthocyanins, monoterpenoids, carotenoids, flavonoids, and flavonols (kaempferol). This review aims to describe the different properties of saffron flower tepals, including their botanical characteristics, phytochemical composition, biological activities, and cosmetology and perfumery uses. [ABSTRACT FROM AUTHOR]

Classical scholars have long held that the saffron in widespread use throughout the ancient Mediterranean was Crocus sativus (Iridaceae), a sterile triploid descendant of the wild Crocus cartwrightianus, and indeed use of Crocus sativus in antiquity has been extensively borne out both by iconographic and phylogenetic studies. Two principal scholars of the Roman world, Dioscorides the physician and Pliny the natural historian, disagreed radically over the virtues and commercial value of saffron crocus from Sicily, with one praising its quality, and the other excoriating it. This study draws on ecophysiology, classical texts, environmental archeology, and phytochemistry to explain this disagreement and its implications. It explores the potential impact of microclimate on crocus cultivation in the ancient Mediterranean and proposes a new species identification for Sicilian crocus: Crocus longiflorus. The identification of Crocus longiflorus as "Sicilian saffron" offers an important corrective to the assumption that Crocus sativus was the sole crocus species of commercial value in the ancient Mediterranean and renews attention to the economic potential and utility of an indigenous southern Italian species overlooked in classical and later scholarship. [ABSTRACT FROM AUTHOR]

Repurposing saffron (Crocus sativus) waste presents a sustainable strategy for generating high-value products within the bioeconomy framework. Typically, flower components are discarded after stigma harvest, resulting in significant waste—350 kg of tepals per kilogram of stigmas. This research employed a comprehensive approach, integrating bioactivity studies (in vitro and in silico) with Life Cycle Assessment (LCA) evaluations, to extract and assess bioactive compounds from C. sativus tepals sourced in Tuscany, Italy. Phytochemical characterization using UPLC-MS/MS revealed a high abundance and variety of flavonoids in the hydro-ethanolic extract (CST). The antioxidant capacity was validated through various assays, and the ability to mitigate H2O2-induced oxidative stress and enhance fermentation was demonstrated in Saccharomyces cerevisiae. This study reports that C. sativus tepals extract reduces oxidative stress and boosts ethanol fermentation in yeast, paving the way for applications in the food and biofuels sectors. Further validation in RAW 264.7 macrophages confirmed CST's significant anti-inflammatory effects, indicating its potential for pharmaceutical, cosmeceutical, and nutraceutical applications. In silico studies identified potential targets involved in antioxidant and anti-inflammatory processes, shedding light on possible interaction mechanisms with Kaempferol 3-O-sophoroside (KOS-3), the predominant compound in the extract. The integration of LCA studies highlighted the environmental benefits of this approach. Overall, this research underscores the value of using waste-derived extracts through "green" methodologies, offering a model that may provide significant advantages for further evaluations compared to traditional methodologies and supporting the circular bioeconomy. [ABSTRACT FROM AUTHOR]

Abstract Saffron (Crocus sativus L.) is being embraced as the most important medicinal plant and the commercial source of saffron spice. Despite the beneficial economic and medicinal properties of saffron, the regulatory mechanism of the correlation of TFs and genes related to the biosynthesis of the apocarotenoids pathway is less obvious. Realizing these regulatory hierarchies of gene expression networks related to secondary metabolites production events is the main challenge owing to the complex and extensive interactions between the genetic behaviors. Recently, high throughput expression data have been highly feasible for constructing co-regulation networks to reveal the regulated processes and identifying novel candidate hub genes in response to complex processes of the biosynthesis of secondary metabolites. Herein, we performed Weighted Gene Co-expression Network Analysis (WGCNA), a systems biology method, to identify 11 regulated modules and hub TFs related to secondary metabolites. Three specialized modules were found in the apocarotenoids pathway. Several hub TFs were identified in notable modules, including MADS, C2H2, ERF, bZIP, HD-ZIP, and zinc finger protein MYB and HB, which were potentially associated with apocarotenoid biosynthesis. Furthermore, the expression levels of six hub TFs and six co-regulated genes of apocarotenoids were validated with RT-qPCR. The results confirmed that hub TFs specially MADS, C2H2, and ERF had a high correlation (P

Saffron cultivation is important in global agriculture and is mainly flourishing in Mediterranean climates. Although it originated in Asia Minor, it is widely grown in regions such as Iran, India, Spain, Morocco, Greece, and Italy. Labour-intensive harvesting, mainly by hand, characterises its production and offers substantial employment opportunities in cultivating areas. However, traditional saffron-producing countries such as Spain, Italy, and Greece have witnessed declining production due to labour demands and competition from low-wage countries. Mechanization remains unfeasible due to the delicate nature of the plant. To revitalise saffron cultivation, efforts have been focused on reducing labour costs, improving productivity, and improving quality through innovative techniques, such as soilless crops. In this study, the productivity and quality of saffron was evaluated in a soilless culture and three fertigation doses were evaluated: a control, consisting of Sonneveld and Voogt’s standard nutrient solution, and two treatments with two supplemented solutions, 30% K and 30% Ca. The results indicated that the solution with 30% K obtained higher corm productivity, as well as better quality saffron, as all the products of this treatment were included in Category I according to the ISO 3632 standard, while the quality of saffron grown with the control solution was lower.

Introduction: Medicinal herbs used in traditional diabetes treatment are a rich source of anti-diabetic compounds. Pancreatic α-amylase inhibitors offer an effective strategy to reduce postprandial hyperglycemic levels via control of starch degradation. In this context, our study for the first time investigates the effect of Crocus sativus stamens extracts on α-amylase inhibition. Material and methods: The hydromethanolic and hydroethanolic extracts were obtained by macerating the dried stamen powder with methanol/water or ethanol/water, respectively. The total phenolic content of the stamen extracts was assessed using the Folin-Ciocalteu reagent method, while the total flavonoid content was determined using the Aluminum Chloride method. Phytochemicals were further quantified and identified using HPLC-DAD. For evaluation of hypoglycemic activity, in vitro α-amylase enzyme inhibition was calculated. The results were confirmed in vivo using an oral starch tolerance test in both normal and diabetic rats. Results: Our findings demonstrated a higher level of polyphenols and flavonoids in the hydroethanolic extract. Important flavonoids found were kaempferol, rutin, and vanillic acid, while prominent carotenoids contained trans- and cis-crocins. The in vitro study showed that both hydromethanolic and hydroethanolic extracts had considerable inhibitory effects, with maximum inhibitions of approximately 83% and 89%, respectively. In vivo tests indicated that both extracts effectively lowered peak blood glucose and area under the curve in both normal and diabetic rats following oral starch treatment. The obtained results are also supported by a docking study. Conclusion: These findings imply that C. sativus stamens possess a distinctive capability to reduce postprandial blood glucose levels. This effect is likely mediated through the inhibition of α-amylase, presenting a novel dietary avenue for managing diabetes. [ABSTRACT FROM AUTHOR]

Obesity is a pressing problem worldwide for which standard therapeutic strategies have limited effectiveness. The use of natural products seems to be a promising approach to alleviate obesity and its associated complications. The tepals of Crocus sativus (Cr) plant, usually wasted in saffron production, are an unexplored source of bioactive compounds. Our aim was to elucidate the mechanisms of Cr tepals extract in obesity by investigating its effects on adipocyte differentiation, visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) hypertrophy, and lipid metabolism in an animal model of diet‐induced obesity. To this end, mouse 3T3‐F442A preadipocytes were treated with Cr tepals extract and the expression of adipocyte differentiation genes was determined. Caloric intake, body mass, triglycerides, systemic insulin sensitivity, histology, insulin signaling, and lipid metabolism in VAT and SAT were analyzed in mice fed a 60% fat diet for 14 weeks and treated orally with Cr tepals extract during the last 5 weeks of the diet. We demonstrated for the first time that Cr tepals extract inhibits adipocyte differentiation in vitro. The animal model confirmed that oral treatment with Cr tepals extract results in weight loss, improved systemic insulin sensitivity, lower triglycerides, and improved lipid peroxidation. The suppressive effect of Cr tepals extract on adipocyte hypertrophy and inflammation was observed only in SAT, which, together with preserved SAT insulin signaling, most likely contributed to improved systemic insulin sensitivity. Our results suggest the functionality of SAT as a possible target for the treatment of obesity and its complications. [ABSTRACT FROM AUTHOR]

Crocussativus is a valuable plant due to the presence of apocarotenoids in its stigma. Considerable work has been done in the past to understand the apocarotenoid biosynthetic pathway in saffron. However, the reports on understanding the regulation of flowering at the post-transcriptional level are meagre. The study aimed to discover the candidate miRNAs, target genes, transcription factors (TFs), and apocarotenoid biosynthetic pathway genes associated with the regulation and transition of flowering in C. sativus. In the present investigation, miRNA profiling was performed in flowering and non-flowering corms of saffron, along with expression analysis of apocarotenoid genes and transcription factors involved in the synthesis of secondary metabolites. Significant modulation in the expression of miR156, miR159, miR166, miR172, miR395, miR396, miR399, and miR408 gene families was observed. We obtained 36 known miRNAs (26 in flowering and 10 in non-flowering) and 64 novel miRNAs (40 in flowering and 24 in non-flowering) unique to specific tissues in our analysis. TFs, including CsMADS and CsMYb, showed significant modulation in expression in flowering tissue, followed by CsHB. Additionally, the miRNAs were predicted to be involved in carbohydrate metabolism, phytohormone signalling, regulation of flower development, and response to stress, cold, and defence. The comprehensive study has enhanced our understanding of the regulatory machinery comprising factors like phytohormones, abiotic stress, apocarotenoid genes, transcription factors, and miRNAs responsible for the synthesis of apocarotenoids and developmental processes during and after flowering. [ABSTRACT FROM AUTHOR]

Saffron cultivation is important in global agriculture and is mainly flourishing in Mediterranean climates. Although it originated in Asia Minor, it is widely grown in regions such as Iran, India, Spain, Morocco, Greece, and Italy. Labour-intensive harvesting, mainly by hand, characterises its production and offers substantial employment opportunities in cultivating areas. However, traditional saffron-producing countries such as Spain, Italy, and Greece have witnessed declining production due to labour demands and competition from low-wage countries. Mechanization remains unfeasible due to the delicate nature of the plant. To revitalise saffron cultivation, efforts have been focused on reducing labour costs, improving productivity, and improving quality through innovative techniques, such as soilless crops. In this study, the productivity and quality of saffron was evaluated in a soilless culture and three fertigation doses were evaluated: a control, consisting of Sonneveld and Voogt's standard nutrient solution, and two treatments with two supplemented solutions, 30% K and 30% Ca. The results indicated that the solution with 30% K obtained higher corm productivity, as well as better quality saffron, as all the products of this treatment were included in Category I according to the ISO 3632 standard, while the quality of saffron grown with the control solution was lower. [ABSTRACT FROM AUTHOR]

The effect of Crocus sativus on several disorders has been discussed or even confirmed, but the efficacy of this herb on the female reproductive system has not been well presented. In this regard, this systematic review comprehensively discussed the efficacy of C. sativus and its main phytochemical compounds on the female reproductive system and its disorders for the first time. In this systematic review, scientific databases, including PubMed, Web of Sciences, Google Scholar, Scopus, and Scientific Information Database, were explored profoundly. In vivo, in vitro, and human studies published until the end of July 2023, which had investigated the pharmacological properties of C. sativus, crocin, crocetin, safranal, or picrocrocin on the female reproductive system, were selected. A total of 50 studies conducted on the effect of C. sativus on the female reproductive system were acquired. These studies confirmed the efficacy of C. sativus or its main phytochemical ingredients in several aspects of the female reproductive system, including regulation of sex hormones, folliculogenesis, ovulation, and protection of the ovary and uterus against several oxidative stress. Several retrieved studies indicated that this herb also can alleviate the symptoms of patients suffering from dysmenorrhea, premenstrual syndrome, menopause, polycystic ovary disease (PCOD), and sexual dysfunction. Furthermore, it is a promising candidate for future studies or even trials regarding ovarian and cervical cancers. This review concluded that C. sativus can improve the symptoms of several female reproductive system disorders, which is particularly due to the presence of phytochemical ingredients, such as crocin, crocetin, and safranal. [ABSTRACT FROM AUTHOR]

Saffron is an extremely high-value spice made from the dried stigmas of the saffron crocus (Crocus sativus). The saffron crocus flowers in the fall, grows vegetatively during the winter and spring, and is dormant during the summer. It is native to the Mediterranean region and most of the global saffron crop is produced in the Mediterranean and western Asia. Summer rainfall is rare in these regions. However, saffron crocus is becoming increasingly popular as a niche crop in the northeastern United States and other areas where summer rainfall is common. This study evaluated four methods of weed control during summer dormancy to determine the relative costs and the effects on saffron yield. Hand cultivation (HC) was the most expensive method and resulted in significantly decreased saffron yields. Covering the saffron crocus field with a light-proof plastic tarp (occultation) was the least expensive and had no effect on saffron yield. Annual planting of saffron crocus also had no effect on saffron yield but required more labor than occultation. Intercropping basil (Ocimum basilicum) resulted in the highest revenue per unit area and did not negatively affect yields of either saffron or basil. Based on this study intercropping is recommended for summer weed management for producers seeking to maximize revenue per unit area, while occultation is recommended for producers seeking to minimize production costs.

BackgroundAnimal and human studies have demonstrated that the saffron and the active components of saffron, including crocin, crocetin, and safranal, possess anti-inflammatory, antioxidant, and immunomodulatory properties. In this meta-analysis, the preclinical evidence and potential mechanism of saffron were explored in an animal model of ovalbumin-induced asthma.MethodsStudies related to saffron and its constituents in an animal model of ovalbumin-induced asthma from the beginning to March 2024 were searched from Scopus, PubMed, and Web of Science databases. The methodological quality of the studies was evaluated using the 15-item CAMARADES checklist. Data analysis was performed using STATA software version 17.ResultsThirteen studies with 536 animals (268 animals in the intervention group and 268 animals in the ovalbumin-induced group) were analyzed. The meta-analysis findings demonstrated that saffron and its constituents played a significant role in reducing total WBC, eosinophil, lymphocyte, and monocyte counts. Moreover, saffron showed a significant decrease in the levels of IL-4, IL-5, IL-13, IgE, histamine, endothelin, nitric oxide, and nitrite. Moreover, saffron was found to elevate EC50 thresholds and lower maximum response rates in experimental animals. The analysis revealed a significant identification of modulation in endoplasmic reticulum (ER) stress markers and miRNAs pathways.ConclusionSaffron and its components may impact ovalbumin-induced asthma model in animals through anti-inflammatory, antioxidant, and immunomodulatory pathways, as well as improving pulmonary function and modulating ER stress markers and miRNAs pathways. As a result, saffron should be considered for further clinical trials in individuals suffering from asthma.

Objective(s): The effects of Crocus sativus, safranal, and pioglitazone on aerosolized paraquat (PQ)-induced systemic changes were examined. Materials and Methods: Control (Ctrl) and PQ groups of rats were exposed to saline or PQ (27 and 54 mg/m3, PQ-L and PQ-H) aerosols eight times on alternate days. Nine PQ-H groups were treated with dexamethasone (0.03 mg/kg/day, Dexa), two doses of C. sativus extract (20 and 80 mg/kg/day, CS-L and CS-H), safranal (0.8 and 3.2 mg/kg/day, Saf-L and Saf-H), pioglitazone (5 and 10 mg/kg/day, Pio-L and Pio-H), and the combination of low dose of the pioglitazone and extract or safranal (Pio + CS and Pio + Saf) after the end of PQ exposure. Results: Interferon-gamma (INF-γ), interleukin 10 (IL-10), superoxide dismutase (SOD), catalase (CAT), and thiol serum levels were reduced, but tumor necrosis factor (TNF-α), malondialdehyde (MDA), and total and differential WBC were increased in both PQ groups (P

Abstract Metabolic syndrome (MetS) is a cluster of clinical syndromes that is closely associated with an elevated risk of developing atherosclerotic cardiovascular disease. In a series of animal experiments and clinical trials, crocus sativus and its component crocin have demonstrated promising hypoglycemic effects. However, there is currently insufficient evidence regarding their impact on cardiometabolic parameters. Our study aimed to assess the impact of Crocus sativus and crocin on glycemic control in individuals with metabolic syndrome and associated disorders, as well as their potential effects on improving cardiometabolic parameters. We searched Cochrane Library, PubMed, Embase, and Web of Science databases to ascertain the pertinent randomized controlled trials (RCTs) until December 30, 2023. Q-test and I2 statistics were utilized to evaluate heterogeneity among the included studies. Data were merged using a random-effects model and presented as (WMD) with a 95% confidence interval (CI). The current comprehensive review and meta-analysis, encompassing 13 RCTs involving a total of 840 patients diagnosed with metabolic syndrome and associated disorders, demonstrates that Crocus sativus was superior to placebo on Hemoglobin A1c(HbA1c) (WMD: -0.31;95% CI [-0.44,-0.19]. P = 0.002) and systolic blood pressure(SBP) (WMD:-7.49;95% CI [-11.67,-3.30]. P = 0.99) respectively. Moreover, Crocus sativus improved fasting blood glucose (FBG) (WMD:-7.25;95% CI [-11.82, -2.57]. P = 0.002) when used crocin and on other chronic diseases. Crocus sativus reduced the total cholesterol (TC) among the metabolic syndromepatients (WMD:-13.64;95%CI [-26.26, -1.03]. P = 0.03). We demonstrated that Crocus sativus exerts beneficial effects on glycemic control and cardiometabolic parameters in individuals with metabolic syndrome and related disorders.

Crocus sativus L. was used for the treatment of a wide range of disorders in traditional medicine. Due to the extensive protective and treatment properties of C. sativus and its constituents in various diseases, the purpose of this review is to collect a summary of its effects, on experimental studies, both in vitro and in vivo. Databases such as PubMed, Science Direct, and Scopus were explored until January 2023 by employing suitable keywords. Several investigations have indicated that the therapeutic properties of C. sativus may be due to its anti-oxidant and anti-inflammatory effects on the nervous, cardiovascular, immune, and respiratory systems. Further research has shown that its petals also have anticonvulsant properties. Pharmacological studies have shown that crocetin and safranal have anti-oxidant properties and through inhibiting the release of free radicals lead to the prevention of disorders such as tumor cell proliferation, atherosclerosis, hepatotoxicity, bladder toxicity, and ethanol induced hippocampal disorders. Numerous studies have been performed on the effect of C. sativus and its constituents in laboratory animal models under in vitro and in vivo conditions on various disorders. This is necessary but not enough and more clinical trials are needed to investigate unknown aspects of the therapeutic properties of C. sativus and its main constituents in different disorders.

Crocus sativus (saffron) is a globally autumn-flowering plant, and its stigmas are the most expensive spice and valuable herb medicine. Crocus specialized metabolites, crocins, are biosynthesized in distant species, Gardenia (eudicot) and Crocus (monocot), and the evolution of crocin biosynthesis remains poorly understood. With the chromosome-level Crocus genome assembly, we revealed that two rounds of lineage-specific whole genome triplication occurred, contributing important roles in the production of carotenoids and apocarotenoids. According to the kingdom-wide identification, phylogenetic analysis, and functional assays of carotenoid cleavage dioxygenases (CCDs), we deduced that the duplication, site positive selection, and neofunctionalization of Crocus-specific CCD2 from CCD1 members are responsible for the crocin biosynthesis. In addition, site mutation of CsCCD2 revealed the key amino acids, including I143, L146, R161, E181, T259, and S292 related to the catalytic activity of zeaxanthin cleavage. Our study provides important insights into the origin and evolution of plant specialized metabolites, which are derived by duplication events of biosynthetic genes.

Metabolic syndrome (MetS) is a cluster of clinical syndromes that is closely associated with an elevated risk of developing atherosclerotic cardiovascular disease. In a series of animal experiments and clinical trials, crocus sativus and its component crocin have demonstrated promising hypoglycemic effects. However, there is currently insufficient evidence regarding their impact on cardiometabolic parameters. Our study aimed to assess the impact of Crocus sativus and crocin on glycemic control in individuals with metabolic syndrome and associated disorders, as well as their potential effects on improving cardiometabolic parameters. We searched Cochrane Library, PubMed, Embase, and Web of Science databases to ascertain the pertinent randomized controlled trials (RCTs) until December 30, 2023. Q-test and I2 statistics were utilized to evaluate heterogeneity among the included studies. Data were merged using a random-effects model and presented as (WMD) with a 95% confidence interval (CI). The current comprehensive review and meta-analysis, encompassing 13 RCTs involving a total of 840 patients diagnosed with metabolic syndrome and associated disorders, demonstrates that Crocus sativus was superior to placebo on Hemoglobin A1c(HbA1c) (WMD: -0.31;95% CI [-0.44,-0.19]. P = 0.002) and systolic blood pressure(SBP) (WMD:-7.49;95% CI [-11.67,-3.30]. P = 0.99) respectively. Moreover, Crocus sativus improved fasting blood glucose (FBG) (WMD:-7.25;95% CI [-11.82, -2.57]. P = 0.002) when used crocin and on other chronic diseases. Crocus sativus reduced the total cholesterol (TC) among the metabolic syndromepatients (WMD:-13.64;95%CI [-26.26, -1.03]. P = 0.03). We demonstrated that Crocus sativus exerts beneficial effects on glycemic control and cardiometabolic parameters in individuals with metabolic syndrome and related disorders. [ABSTRACT FROM AUTHOR]

Objective(s): The effects of Crocus sativus, safranal, and pioglitazone on aerosolized paraquat (PQ)- induced systemic changes were examined. Materials and Methods: Control (Ctrl) and PQ groups of rats were exposed to saline or PQ (27 and 54 mg/m3, PQ-L and PQ-H) aerosols eight times on alternate days. Nine PQ-H groups were treated with dexamethasone (0.03 mg/kg/day, Dexa), two doses of C. sativus extract (20 and 80 mg/kg/day, CS-L and CS-H), safranal (0.8 and 3.2 mg/kg/day, Saf-L and Saf-H), pioglitazone (5 and 10 mg/kg/day, Pio-L and Pio-H), and the combination of low dose of the pioglitazone and extract or safranal (Pio + CS and Pio + Saf) after the end of PQ exposure. Results: Interferon-gamma (INF-γ), interleukin 10 (IL-10), superoxide dismutase (SOD), catalase (CAT), and thiol serum levels were reduced, but tumor necrosis factor (TNF-α), malondialdehyde (MDA), and total and differential WBC were increased in both PQ groups (P<0.05 to P<0.001). All measured variables were improved in all treated groups (P<0.05 to P<0.001). The effects of high dose of C. sativus and safranal on measured parameters were higher than dexamethasone (P<0.05 to P<0.001). The effects of Pio + CS and Pio + Saf treatment on most variables were significantly higher than three agents alone (P<0.05 to P<0.001). Conclusion: C. sativus and safranal improved inhaled PQ-induced systemic inflammation and oxidative stress similar to those of dexamethasone and showed synergic effects with pioglitazone suggesting the possible PPARγ receptor-mediated effects of the plant and its constituent. [ABSTRACT FROM AUTHOR]

Background: Cultivation of Crocus sativus (saffron) faces challenges due to inconsistent flowering patterns and variations in yield. Flowering takes place in a graded way with smaller corms unable to produce flowers. Enhancing the productivity requires a comprehensive understanding of the underlying genetic mechanisms that govern this size-based flowering initiation and commitment. Therefore, samples enriched with non-flowering and flowering apical buds from small (< 6 g) and large (> 14 g) corms were sequenced. Methods and results: Apical bud enriched samples from small and large corms were collected immediately after dormancy break in July. RNA sequencing was performed using Illumina Novaseq 6000 to access the gene expression profiles associated with size dependent flowering. De novo transcriptome assembly and analysis using flowering committed buds from large corms at post-dormancy and their comparison with vegetative shoot primordia from small corms pointed out the major role of starch and sucrose metabolism, Auxin and ABA hormonal regulation. Many genes with known dual responses in flowering development and circadian rhythm like Flowering locus T and Cryptochrome 1 along with a transcript showing homology with small auxin upregulated RNA (SAUR) exhibited induced expression in flowering buds. Thorough prediction of Crocus sativus non-coding RNA repertoire has been carried out for the first time. Enolase was found to be acting as a major hub with protein–protein interaction analysis using Arabidopsis counterparts. Conclusion: Transcripts belong to key pathways including phenylpropanoid biosynthesis, hormone signaling and carbon metabolism were found significantly modulated. KEGG assessment and protein–protein interaction analysis confirm the expression data. Findings unravel the genetic determinants driving the size dependent flowering in Crocus sativus. [ABSTRACT FROM AUTHOR]

Saffron is a spice derived from the flower of Crocus sativus L., which has been used for centuries as a coloring and flavoring agent, as well as a source of medicinal compounds. Saffron contains various bioactive constituents, such as crocin, crocetin, safranal, picrocrocin, and kaempferol, that have shown potential benefits for human health. Among them, crocin is the most abundant and characteristic constituent of saffron, responsible for its bright red color and antioxidant properties. One of the most promising applications of saffron and its constituents is in the prevention and treatment of neurological disorders, such as depression, anxiety, Alzheimer's disease, Parkinson's disease, and other brain disorders. Saffron and its constituents have been reported to exert neuroprotective effects through various mechanisms, such as modulating neurotransmitters, enhancing neurogenesis, reducing neuroinflammation, regulating oxidative stress, activating the Nrf2 signaling pathway, and modulating epigenetic factors. Several clinical and preclinical studies have demonstrated the efficacy and safety of saffron and its constituents in improving cognitive function, mood, and other neurological outcomes. In this review, we summarize the current evidence on the therapeutic potential of saffron and its constituents in neurological disorders, from bench to bedside. We also discuss the challenges and future directions for the development of saffron‐based therapies for brain health. [ABSTRACT FROM AUTHOR]

The bulb mite, Rhizoglyphus robini Claparédè has been identified as a common pest attacking saffron corms. This mite is also a saprophytic species that is able to feed on fungi (mycelium). It has also been demonstrated that some soilborn fungi can attack and establish on saffron corms. After related fungi were isolated and identified either in saffron corms and mite bodies, the fungus Fusarium oxysporum Schlecht was the most abundant species in both samples; subsequently, it was used in the experiments. For studying the role of soil-borne fungi on life-table parameters of the mite, an even-aged cohort of eggs was obtained and transferred to experimental units to feed on saffron corm sections in two groups (fungal-infected and non-infected corms) in laboratory conditions. A total of 36 individuals were used for each treatment. The life-history data were analyzed according to the age-stage, two-sex life table model. Results showed that total pre-adult development of R. robini was significantly faster on Fusarium-infected rather than healthy corms (10.91 and 11.48 days respectively, P < 0.05). Adult pre-oviposition period (APOP) was significantly shorter when females were reared on fungal-infested than non-infested corms (1.84 and 2.88 days, respectively, P < 0.05). In addition, survivorship and fecundity of the mite on the two diets indicated higher rates and consequently, the net reproductive rate (R0) and intrinsic rate of increase (r) were significantly higher (P < 0.05 for both parameters) (202.07 and 351.13 offspring for R0 and 0.218 and 0.251 day-1 for r respectively on non-infested and infested diets). According to the results obtained, the soil-borne fungus affected demographic parameters of the bulb mite and it supports the hypothesis that the mite prefers plant tissues infected with soil-borne fungi because these are more suitable hosts. [ABSTRACT FROM AUTHOR]

The reproduction of Crocus sativus L., a sterile triploid plant, is carried out exclusively through corms, whose size determines the saffron yield. The development of daughter corms (DC) is supported by photoassimilates supplied by the leaves as well as by the mother corms (MC). While biomass partitioning during DC development is well studied, growth dynamics in terms of cell number and size, the involved meristems, as well as carbohydrate partition and allocation, are not yet fully understood. We conducted a comprehensive study into saffron corm growth dynamics at the macroscopic and microscopic levels. Variations in carbohydrate content and enzymatic activities related to sucrose metabolism in sources and sinks were measured. Two key meristems were identified. One is involved in vascular connections between DC and MC. The other is a thickening meristem responsible for DC enlargement. This research explains how the previously described phases of corm growth correlate with variations in cell division, enlargement dynamics, and carbohydrate partitioning among organs. Results also elucidated that the end of DC growth relates to a significant drop in MC root biomass, limiting the water supply for the DC growth, and establishing the onset of leaf wilting. The lack of starch accumulation in aged leaf cells is noteworthy, as is the accumulation of lipids. We hypothesize a signaling role of sugars in DC growth initiation, stop, and leaf aging. Finally, we established a predominant role of sucrose synthase as a sucrolytic enzyme in the maintenance of the high flux of carbon for starch synthesis in DC. Together, the obtained results pave the way for the definition of strategies leading to better control of saffron corm development. [ABSTRACT FROM AUTHOR]

• Stigma developmental stages revealed a bi-phase carotenoid biosynthetic pathway. While the synthesis of Lycopene peaks at S2 stage, β- carotene and apocarotenoids peaks at S3 and S4 stages, respectively. • CsPSY2, CsBCH and CsCCD2 genes play a pivotal role in apocarotenoid accumulation. • A gene complex (CsPIF1, CsHY5, CsPSY2) regulates stigma carotenoid biosynthesis. • The results indicate plastid development to S 1 & S 2 stigmas and chromoplast differentiation to S 3 and S 4 stigma stages. ORa gene correlates strongly with chromoplast biogenesis in stigmas. • Based on correlation networking, CsPIF1, CsHY5, and CsPSY2 likely form a complex to regulate carotenoid biosynthesis in stigmas. Integration of the apocarotenoid and transcriptome profiles in developing stigmas of saffron revealed a biphasic pattern of carotenoid biosynthetic pathway. While the synthesis of lycopene peaks at S 2 stage that of β- carotene and Cs LCY-β, peaks at S 3 stage of stigma development. Cs ZCD, Cs GTF, Cs BGSD, Cs ADH and Cs VDE, responsible for the oxidative cleavage of carotenoids to produce apocarotenoids, showed highest expression in S 4 stage stigmas. Cs PSY2, Cs BCH and Cs CCD2 showed the highest correlation with apocarotenoid accumulation in the stigmas, indicating them to be the key genes in carotenoid biosynthesis. Our results indicate active plastid development in yellow and orange stigmas and active chromoplast differentiation in red (S 3) and scarlet (S 4) stigmas. The expression of the OR-a gene in the stigmas was found to be strongly related to chromoplast biogenesis. On the basis of correlation networking, we suggest the involvement of a repression-activation transcriptional complex comprised of the Cs PIF1, Cs HY5 and Cs PSY2 genes in regulating the biosynthesis of carotenoids in the saffron stigmas. [ABSTRACT FROM AUTHOR]

Many plant species of family Iridaceae like Crocus, Gladiolus, Iris and Freesia are grown worldwide for their commercially valued flowers and flowering product. All these plants along with other cultivated members are known to get diseases from multiple pathogens. This leads to greater damage in yield causing huge economic losses to farmers and growers. To understand the disease cycle, their cause and prophylactic measures on broader scale, fungal and bacterial pathogens causing corm rot diseases, blight diseases, leaf spot, wilt diseases and viral diseases are discussed. The survey of the reported literature and their analysis revealed that corm and rhizome rot is most devastating disease in all commercially valued species from this family, worldwide. Fungal pathogen Fusarium oxysporum found to be pathogen of major concern. Viral and bacterial disease is equally recurring problem. Yet, suitable and recommended agronomic practices along with use of fungicides and bactericides have potential in preventing spread of pathogens and diseases to nearby fields. The endeavour of the article is to provide summarised information with potential to be utilised by stakeholders for better disease management and prediction. [ABSTRACT FROM AUTHOR]

Crocus sativus L. was used for the treatment of a wide range of disorders in traditional medicine. Due to the extensive protective and treatment properties of C. sativus and its constituents in various diseases, the purpose of this review is to collect a summary of its effects, on experimental studies, both in vitro and in vivo. Databases such as PubMed, Science Direct, and Scopus were explored until January 2023 by employing suitable keywords. Several investigations have indicated that the therapeutic properties of C. sativus may be due to its anti-oxidant and anti-inflammatory effects on the nervous, cardiovascular, immune, and respiratory systems. Further research has shown that its petals also have anticonvulsant properties. Pharmacological studies have shown that crocetin and safranal have anti-oxidant properties and through inhibiting the release of free radicals lead to the prevention of disorders such as tumor cell proliferation, atherosclerosis, hepatotoxicity, bladder toxicity, and ethanol induced hippocampal disorders. Numerous studies have been performed on the effect of C. sativus and its constituents in laboratory animal models under in vitro and in vivo conditions on various disorders. This is necessary but not enough and more clinical trials are needed to investigate unknown aspects of the therapeutic properties of C. sativus and its main constituents in different disorders. [ABSTRACT FROM AUTHOR]

Crocus sativus (saffron) is a globally autumn-flowering plant, and its stigmas are the most expensive spice and valuable herb medicine. Crocus specialized metabolites, crocins, are biosynthesized in distant species, Gardenia (eudicot) and Crocus (monocot), and the evolution of crocin biosynthesis remains poorly understood. With the chromosome-level Crocus genome assembly, we revealed that two rounds of lineage-specific whole genome triplication occurred, contributing important roles in the production of carotenoids and apocarotenoids. According to the kingdom-wide identification, phylogenetic analysis, and functional assays of carotenoid cleavage dioxygenases (CCDs), we deduced that the duplication, site positive selection, and neofunctionalization of Crocus -specific CCD2 from CCD1 members are responsible for the crocin biosynthesis. In addition, site mutation of CsCCD2 revealed the key amino acids, including I143, L146, R161, E181, T259, and S292 related to the catalytic activity of zeaxanthin cleavage. Our study provides important insights into the origin and evolution of plant specialized metabolites, which are derived by duplication events of biosynthetic genes. A chromosome-level Crocus genome and two rounds of species-specific WGT events were reported. The recent Crocus WGT event drives the evolution of CsCCD2 gene from the CsCCD1 gene. [Display omitted] [ABSTRACT FROM AUTHOR]

The phenomenon of wound healing encompasses a coordinated sequence of cellular and biochemical phases collaborating synergistically to promote the restoration of the injured tissue. Tissue repair is complex, posing challenges in wound management. Healing involves three phases: inflammatory, proliferative, and remodeling. Treatments include antibiotics, antiseptics, and extracts, but synthetic drugs have limitations. There is growing interest in plant-based formulations for effective wound treatment. Medicinal plants are increasingly recognized for their wound healing potential with lower side effects, particularly in diabetic, infected, or open wounds, supported by studies highlighting various identified mechanisms for improved healing. Medicinal plants such as Allium sativum, Boerhavia diffusa, Calendula officinalis, Crocus sativus, Curcuma longa L., Glycyrrhiza glabra L., Melaleuca alternifolia, Woodfordia fruticosa, etc. have demonstrated wound healing properties and have proven effective in treating wounds. This review highlights medicinal plants in wound healing, emphasizing In-vivo models, specifically examining their effectiveness in excision and incision wound healing. [ABSTRACT FROM AUTHOR]

Crocus sativa, saffron, is a herbaceous plant native to the Mediterranean region (Greece) and is cultivated in several countries, including Iran, Spain, Italy, Greece, India and Morocco. The part used is the stigma which mainly contain carotenoid-like compounds such as crocin and crocetin as well as picrocrocin and safranal. Saffron has a long history in traditional medicine. It has been used to treat insomnia, asthma, whooping cough, depression, anxiety, heartburn and to reduce cough. Pharmacologically, the drug is responsible for several activities including antioxidant, antibacterial, antidepressant and anxiolytic properties. Saffron also exerts effects on cognitive function and showed antidiabetic and anticancer activities (in vitro and in vivo). Clinically, studies carried out with saffron carotenoids extracts have demonstrated statistically significant results in both glaucoma and AMD. Acute toxicity tests show that saffron, safranal and crocin are mild to non-toxic agents. Other studies have reported that effective doses of saffron used in clinical practice are significantly lower than toxic doses. [ABSTRACT FROM AUTHOR]

Corm rot caused by pathogen Fusarium oxysporum is the most devastating disease of saffron. In the present study, previously isolated and characterized Bacillus sp. strain D5 (Bar D5) and Bacillus amyloliquefaciens W2 (Bam W2), native to Crocus sativus, have been compared to select the potential biostimulant for saffron cultivation. Comparative evaluation under the same conditions was done in-vitro, for various plant growth promoting (PGP) activities and in-planta for growth promotion and reduction of most devastating disease (corm rot) caused by the pathogenic fungus Fusarium oxysporum R1 (Fox R1). Bar D5 emerged as a clear winner in in-vitro and in-planta evaluations. In in-vitro PGP activities, Bar D5 had supremacy with phosphate solubilization > by 3.9 ± 0.04 folds, ammonia production > by 1.4 ± 0.09 folds, IAA production > by 1.5 ± 0.02 folds, amylase production > by 2.8 ± 0.1 folds and cellulose production by 1.4 ± 0.08 folds higher than Bam W2. However, siderophore and protease production were higher in Bam W2 by 1.9 ± 0.11 and 1 ± 0.06 folds, respectively, when compared to Bar D5. In pot assays, Bar D5 primed corms accumulated higher biomass and exhibited enhanced resistance by induction of various defense enzymes. The activity of defense related enzymes at 5 dpi, such as phenylalanine-ammonia lyase (PAL 1.2 ± 0.07 folds), peroxidase (PO 2 ± 0.04 folds), polyphenol oxidase (PPO 1.4 ± 0.09 folds), lipoxygenase (LOX 1.50.05 ± folds), and β,1-3 glucanase (GLU 1.6 ± 0.3 folds) was higher in Bar D5 primed and Fox R1 inoculated corms compared to Bam W2 primed and Fox R1 inoculated corms. In addition, Bar D5 helped plants to accumulate biomass better than Bam W2, both in the presence and absence of the pathogen. Bar D5 is beyond doubt the potential biostimulant for saffron cultivation. [ABSTRACT FROM AUTHOR]

With the increasing concerns among consumers regarding food quality and safety, the demand for reliable food authentication methods has risen significantly. This study aims to address this challenge by utilizing UV–visible spectroscopy as a fast and accurate analysis tool for geographically discriminating Moroccan saffron samples from five different regions. Analysis of the saffron samples was carried out using a UV–visible spectrophotometer and a quartz cell, over the wavelength range 200–700 nm, with an optical path length of 1 cm. The spectral data were analyzed using three chemometric models: principal component analysis (PCA), support vector machine (SVM) and linear discriminant analysis (LDA). Our findings revealed that samples from the TALIOUINE, ANERGUI, and AIT-MAZIGH regions were categorized as grade I, while samples from the AIT-OUMDIS region fell under grade II, and saffron from the TAZENAKHT region was classified as grade III. Furthermore, we employed LDA and SVM techniques to successfully classify the synchronous spectra of 120 saffron samples. Notably, the best-performing results were yielded by SVM, achieving a remarkable 97.89% accuracy in correctly classifying the samples when using spectral data processed with the standard normal variate method. This study emphasizes the potential and applicability of UV–Vis spectroscopy as a promising technique for effectively classifying and assessing the quality of saffron samples, taking into account their variety and production method. [ABSTRACT FROM AUTHOR]

Crocins comprise a family of hydrophilic carotenoids with pharmacological properties that are produced in significant quantities in stigma of Crocus sativus. Although the biosynthesis pathway of crocins has been sufficiently elucidated, there is a paucity of information regarding how transcription factors (TFs) regulate crocin biosynthesis in various stigma developmental stages. WRKY TFs play a role in modulating carotenoid/apocarotenoid metabolism. To provide an overview of the WRKY family in Crocus sativus (CsWRKY) and characterize candidate CsWRKY TFs involved in the biosynthesis of crocins, CsWRKY genes were identified from RNA-sequenced stigma at different developmental stages. A phylogenetic analysis was performed to characterize their evolutionary interrelatedness. A coexpression analysis of CsWRKY genes and crocin biosynthesis-related genes was performed. A quantitative real-time polymerase chain reaction was used to corroborate the expression level of CsWRKY TFs in various tissue and at different developmental stages. A total of 34 CsWRKY TFs were identified from the stigma of C. sativus. The CsWRKY TFs, together with their orthologs from Arabidopsis, were clustered into group I, II, or III following phylogenetic analysis. A correlation analysis revealed that the expressions of the TFs CsWRKY1, CsWRKY2, CsWRKY8, CsWRKY10, CsWRKY15, and CsWRKY28 were strongly related to the expression of crocin biosynthesis-related genes CsBCH, CsCCD2L, CsALDH, and CsUGT. CsWRKY2, CsWRKY15, and CsWRKY28 exhibited identical motifs and were stratified into group IId. Transcript levels of candidate CsWRKY genes were higher in stigma than in other tissues and were proportional to the crocin content.

Saffron (Crocus sativus L.), a historically significant crop valued for its nutraceutical properties, has been poorly explored from a phytosanitary perspective. This study conducted a thorough examination of viruses affecting saffron samples from Spanish cultivars, using high-throughput sequencing alongside a systematic survey of transcriptomic datasets from Crocus sativus at the Sequence Read Archive. Our analysis unveiled a broad diversity and abundance, identifying 17 viruses across the 52 analyzed libraries, some of which were highly prevalent. This includes known saffron-infecting viruses and previously unreported ones. In addition, we discovered 7 novel viruses from the Alphaflexiviridae, Betaflexiviridae, Potyviridae, Solemoviridae, and Geminiviridae families, with some present in libraries from various locations. These findings indicate that the saffron-associated virome is more complex than previously reported, emphasizing the potential of phytosanitary analysis to enhance saffron productivity.

Introduction: Medicinal herbs used in traditional diabetes treatment are a rich source of anti-diabetic compounds. Pancreatic α-amylase inhibitors offer an effective strategy to reduce postprandial hyperglycemic levels via control of starch degradation. In this context, our study for the first time investigates the effect of Crocus sativus stamens extracts on α-amylase inhibition.Material and methods: The hydromethanolic and hydroethanolic extracts were obtained by macerating the dried stamen powder with methanol/water or ethanol/water, respectively. The total phenolic content of the stamen extracts was assessed using the Folin-Ciocalteu reagent method, while the total flavonoid content was determined using the Aluminum Chloride method. Phytochemicals were further quantified and identified using HPLC-DAD. For evaluation of hypoglycemic activity, in vitro α-amylase enzyme inhibition was calculated. The results were confirmed in vivo using an oral starch tolerance test in both normal and diabetic rats.Results: Our findings demonstrated a higher level of polyphenols and flavonoids in the hydroethanolic extract. Important flavonoids found were kaempferol, rutin, and vanillic acid, while prominent carotenoids contained trans- and cis-crocins. The in vitro study showed that both hydromethanolic and hydroethanolic extracts had considerable inhibitory effects, with maximum inhibitions of approximately 83% and 89%, respectively. In vivo tests indicated that both extracts effectively lowered peak blood glucose and area under the curve in both normal and diabetic rats following oral starch treatment. The obtained results are also supported by a docking study.Conclusion: These findings imply that C. sativus stamens possess a distinctive capability to reduce postprandial blood glucose levels. This effect is likely mediated through the inhibition of α-amylase, presenting a novel dietary avenue for managing diabetes.

Introduction: Crocus sativus (C. sativus), known as saffron, has been recognized for its medicinal benefits since ancient times. The plant was first introduced to the Chongming Island region of Shanghai, China, in 1980. Chinese Saffron, derived from this plant, is one of China's most valuable traditional herbs, yielding about 1 ton in 2013. Traditional Chinese Medicine (TCM) has used this plant as an antianginal for a long time. The plant has been extensively studied for its pharmacological benefits in treating obesity and gastrointestinal problems. This study aimed to conduct a comprehensive phytochemical analysis of C. sativus stigma (saffron) ethanolic extract using LCMS/MS, quantify its major compound, i.e., trans-4-GG-crocin, and study its acute toxicity. In addition, this study aimed to investigate the effect of the extract on anti-obesity in a high-fat diet-induced zebrafish model. Methods: Before the anti-obesity study, we conducted a comprehensive profiling of saffron ethanolic extract using UHPLC-ESI-MS/MS in both positive and negative modes. Then, the principal constituent, i.e., trans-4-GG-crocin, was quantified using HPLC with a UV detector. The study was followed by an acute toxicity study for 72 h to evaluate the safety profile of this extract using five concentrations of extract, from 93.75–1500 mg/L. Six groups of wild-type zebrafish were then segregated according to food regimen, i.e., (1) control (5 mg artemia/fish/day), (2) overfeeding (60 mg of artemia/fish/day), (3–6) high-fat diet (5 mg artemia+30 mg egg yolk/fish/day), then starting week 9–16, groups 4–6 were received (4) 50 mg/L, (5) 100 mg/L and (6) 200 mg/L of saffron extract. Results: Negative mode analysis revealed twenty phytoconstituents, while twenty-two were found in positive mode LCMS/MS analysis. The extract contained 536.91 µg/g of trans-4-GG-crocin. The highest concentration tested in the acute toxicity killed 100% of the testing animals within 24 h, and probit analysis showed that the LC50 of the extract was 1021 mg/L. Eight weeks of treatment with 100 and 200 mg/L of saffron significantly reduced the body mass index of the high-fat diet-induced zebrafish model (p

Repurposing saffron (Crocus sativus) waste presents a sustainable strategy for generating high-value products within the bioeconomy framework. Typically, flower components are discarded after stigma harvest, resulting in significant waste—350 kg of tepals per kilogram of stigmas. This research employed a comprehensive approach, integrating bioactivity studies (in vitro and in silico) with Life Cycle Assessment (LCA) evaluations, to extract and assess bioactive compounds from C. sativus tepals sourced in Tuscany, Italy. Phytochemical characterization using UPLC-MS/MS revealed a high abundance and variety of flavonoids in the hydro-ethanolic extract (CST). The antioxidant capacity was validated through various assays, and the ability to mitigate H2O2-induced oxidative stress and enhance fermentation was demonstrated in Saccharomyces cerevisiae. This study reports that C. sativus tepals extract reduces oxidative stress and boosts ethanol fermentation in yeast, paving the way for applications in the food and biofuels sectors. Further validation in RAW 264.7 macrophages confirmed CST’s significant anti-inflammatory effects, indicating its potential for pharmaceutical, cosmeceutical, and nutraceutical applications. In silico studies identified potential targets involved in antioxidant and anti-inflammatory processes, shedding light on possible interaction mechanisms with Kaempferol 3-O-sophoroside (KOS-3), the predominant compound in the extract. The integration of LCA studies highlighted the environmental benefits of this approach. Overall, this research underscores the value of using waste-derived extracts through “green” methodologies, offering a model that may provide significant advantages for further evaluations compared to traditional methodologies and supporting the circular bioeconomy.

• Eco-friendly Gold NPs was prepared using Crocus Sativus extract. • Novel combination of this NPs provide biocompatibility and anti-oxidant efficacy. • Biocompatible Nanoparticle provides effective anti-inflammatory action by Rat model. • These materials could be used as effective therapeutic agent for Anti-depression action. Depression is a unipolar condition caused by a brain monoamine deficit that affects emotion, thinking, and function. Depression treatments involve limitations such poor absorption, a delay in therapeutic efficacy, unwanted side effects, and high-dose toxicity. A simple, affordable, clean, non-toxic, and environmentally friendly nanoparticle manufacturing method was used to fix this. This research created C.sativus gold nanoparticles (CS-AuNPs) from Crocus sativus extract and auric chloride (HAuCl 4). CS-AuNPs was spherical, 11–20 nm in size, and 550 nm absorbent after UV–vis, XRD, FTIR, SEM, and TEM analysis. Saffron action is increased using gold nanoparticles, a non-toxic drug delivery vehicle. Crocins, crocetin, and safranal are important antidepressants. Crocus sativus has been shown to effectively reduce proinflammatory cytokines mRNA expression (IL-1β, IL-6, and TNF-α) and inhibit NF-κB activation. With an IC 50 of 256 μg/ml for DPPH, IC 50 of 300 μg/ml for NBT assay and 90 % cell survival in cytotoxicity tests, this chemical is safe and effective as an antidepressant. The new study showed that C.sativus has therapeutic promise and that converting it from extract to nanoparticles will benefit depression treatment. [ABSTRACT FROM AUTHOR]

Key message: CstMYB1R1 acts as a positive regulator of Crocus anthocyanin biosynthesis and abiotic stress tolerance which was experimentally demonstrated through molecular analysis and over-expression studies in Crocus and Nicotiana. Regulatory mechanics of flavonoid/anthocyanin biosynthesis in Crocus floral tissues along the diurnal clock has not been studied to date. MYB proteins represent the most dominant, functionally diverse and versatile type of plant transcription factors which regulate key metabolic and physiological processes in planta. Transcriptome analysis revealed that MYB family is the most dominant transcription factor family in C. sativus. Considering this, a MYB-related REVEILLE-8 type transcription factor, CstMYB1R1, was explored for its possible role in regulating Crocus flavonoid and anthocyanin biosynthetic pathway. CstMYB1R1 was highly expressed in Crocus floral tissues, particularly tepals and its expression was shown to peak at dawn and dusk time points. Anthocyanin accumulation also peaked at dawn and dusk and was minimum at night. Moreover, the diurnal expression pattern of CstMYB1R1 was shown to highly correlate with Crocus ANS/LDOX gene expression among the late anthocyanin pathway genes. CstMYB1R1 was shown to be nuclear localized and transcriptionally active. CstMYB1R1 over-expression in Crocus tepals enhanced anthocyanin levels and upregulated transcripts of Crocus flavonoid and anthocyanin biosynthetic pathway genes. Yeast one hybrid (Y1H) and GUS reporter assay confirmed that CstMYB1R1 interacts with the promoter of Crocus LDOX gene to directly regulate its transcription. In addition, the expression of CstMYB1R1 in Nicotiana plants significantly enhanced flavonoid and anthocyanin levels and improved their abiotic stress tolerance. The present study, thus, confirmed positive role of CstMYB1R1 in regulating Crocus anthocyanin biosynthetic pathway in a diurnal clock-specific fashion together with its involvement in the regulation of abiotic stress response. [ABSTRACT FROM AUTHOR]

Saffron (Crocus sativus L.), being one of the distinguished commercial spice crops in the world, is in demand for its culinary, colorant, and pharmaceutical benefits. In this study, a novel indirect somatic embryogenesis (SE) system was, thus, established for the study of this plant. To this end, firstly, the lateral buds were cultured. Then, the cultures were transformed using Murashige and Skoog (MS) medium supplemented with either 6-benzyladenine (BA: 5 and 10 mg/L), naphthalene acetic acid (NAA: 0, 1, and 2 mg/L), or trans-zeatin (tZ: 0, 0.5, and 1.0 mg/L), before being classified into four structures: white globular (WG), yellow compact nodular (YCN), yellow-brown fragile (YBF), and dark-brown porous (DBP). As soon as BA (10 mg/L) and NAA (2 mg/L) were added, elevated percentages of white globular calli (56.8%) and white globular calli (31.5%) structures were induced. Additionally, 6-benzyladenine (5 mg/L) and naphthalene acetic acid (1 mg/L) allowed the formation of yellow-brown fragile structures, and the combination of 6-benzyladenine (10 mg/L) with trans-zeatin (1 mg/L) formed the DBP structures. After three months, the white globular calli were incubated using the MS basal medium, before being augmented with thidiazuron (TDZ: 1 mg/L) and picloram (PIC: 2 mg/L), from which 60% of the cases matured into shoots and, ultimately, cormlets. Morphoanatomical analyses also showed that the white globular calli cells were closely arranged, as they had a dense cytoplasm, a significant vascular differentiation, and embryoids. Furthermore, the yellow compact nodular structures were characterized by a strong differentiation capacity and contained many meristematic cells with high caryomitosis centers. We observed that the yellow-brown fragile calli had looser cell arrangements, with a vascular structure located on the protoderm edge, while there was no obvious cellular arrangement in the dark-brown porous structures. The induction of the adventitious buds in vivo on the MS medium that was supplemented with thidiazuron and picloram accordingly demonstrated the highest rates (60%) of white globular calli. [ABSTRACT FROM AUTHOR]

Background and Aim: Crocus sativus also known as saffron, is one of the most popular traditional plants. This study aims to evaluate the efficacy and safety of saffron extracts in ADHD. Method: This study includes clinical trial studies that assessed the efficacy and/or safety of saffron in ADHD patients. Non-English papers, review articles, commentaries, letters, observational studies, thesis, animal studies, in-vitro studies, and conference abstracts were not included. The risk of bias in randomized studies was evaluated based on the Cochrane RoB.2, and risk of bias in pre-post intervention studies was assessed using the ROBINS-I tool. Results: Four studies met our inclusion criteria with a total of 118 patients. The results manifested an efficient role of saffron as either an adjuvant therapy to MPH or a single therapy against ADHD, without significant safety issues. Discussion: Saffron demonstrates promise in improving ADHD symptoms, with an acceptable safety profile. Future well-designed multicentral studies are suggested. [ABSTRACT FROM AUTHOR]

Purpose: Two major developments have taken place in the agri-food industry in recent decades. First, valorization of the plant by-products can contribute significantly for ensuring sustainability, especially applying green technologies for recovery of bio- and/or techno-functional compounds. Second, substitution of synthetic food additives, particularly colorants, by their natural counterparts is of utmost importance to meet consumer demands. The aim of this study was to evaluate a new process for green extraction of polyphenols from Crocus sativus tepals, the primary by-product of saffron spice production. Methods: A new strategy for recovery of polyphenols from saffron tepals was studied. An extraction process combining two subcritical water extractions steps with enzymatic treatment incorporated between them was established on a pilot scale. Liquid chromatography–mass spectrometry was used for polyphenols profile characterisation. The co-pigmentation efficiency of the polyphenol extract and its effect on thermal stability of strawberry anthocyanins were evaluated in model solutions. Results: An approximately fourfold increase in the polyphenolic yield was achieved with the new combined extraction method. 16 flavonoids, mainly flavonols, were detected in the saffron tepal extract. To the best of our knowledge, the presence of the flavone apigenin has not been described before in Crocus sativus. The addition of saffron tepal polyphenols, acting as co-pigments, significantly reduced the thermal degradation of strawberry anthocyanins. Conclusion: The obtained results demonstrate an innovative approach for the valorisation of saffron tepals through potential application of the by-product polyphenols in fruit processing. [ABSTRACT FROM AUTHOR]

• Saffron stigmas crop generates a large amount of biomass called by-product with high added value. • Saffron floral by-products (tepals, leaves, stamens, styles, spaths, and others) are rich in flavonoids, anthocyanins, carotenoids, and volatile compounds. • Chemical compounds contained in the saffron floral by-products have excellent antioxidant and anti-microbiological activities. • Bioactive compounds of saffron floral by-products are a great coloring and aromatic agents strongly used in industrial formulations. Saffron (Crocus Sativus L.), is the most expensive spice in the world, derived from the red filaments (stigmas) of a sterile triploid geophyte plant that belongs to the Iridaceae family. Saffron stigmas have been traditionally used in food and cosmetic products due to their coloring, flavoring, and aromatic aspects, attributed to their bioactive compounds, namely crocin, picrocrocin, and safranal. After harvesting saffron stigmas, a considerable amount of saffron floral parts so-called by-products are generated: petals, leaves, stamens, and others. Recent scientific studies have focused on cosmetic, therapeutic, and nutraceutical applications of not only saffron stigmas but also their several floral by-products. The chemical compounds contained in the whole saffron flower such as polyphenols, flavonoids, and anthocyanins, are endowed with excellent potentialities like antioxidant, and antimicrobial activities which makes them strongly used in food and cosmetic products. This paper aims to provide a systematic overview of specialized (secondary) metabolites applications of saffron stigmas as well as its by-products in various fields, also, the mechanisms of action of saffron bioactive compounds activities have been explained in this paper. [ABSTRACT FROM AUTHOR]

High-quality genomic DNA is essential for genomic and molecular investigations such as nextgeneration sequencing. However, DNA extraction from medicinal plants like Crocus sativus can be challenging due to their high secondary metabolite content, which can interact with nucleic acids and affect the quality and yield of extraction. This study aimed to optimize the quality and yield of DNA using the cetyltrimethylammonium bromide (CTAB) extraction method from the leaves, stigma, and saffron corm. This new method is easy to use and can be performed using standard equipment and inexpensive reagents. The modifications made to the CTAB lysis buffer in this study, with the addition of SDS, resulted in a yield of 4233 ng/µl of DNA per sample of saffron corm (100 mg). This protocol is efficient and cost-effective for DNA extraction for studies with large samples and limited resources. This method is expected to be widely used for large-scale plant extraction and has a broad application in PCR-based sequencing studies. [ABSTRACT FROM AUTHOR]

Abstract Background Although the mechanism of action of nanoemulsion is still unclear, the modern use of nanoemulsions made from natural extracts as antimicrobial and anti-aflatoxigenic agents represents a potential food preservation and a safety target. Methods Two natural nanoemulsion extracts of Crocus sativus (the saffron flower) and Achillea millefolium (the yarrow flower) were produced in the current study using a low-energy method that included carboxymethylcellulose and Arabic gum. The synthesized nanoemulsion was fully identified by different analytical methods. Detection of the volatile content was completed using GC-MS analysis. The antioxidant potential, and phenolic compounds content were analyzed in the extractions. The synthesized nanoemulsions were screened for their antimicrobial potential in addition to their anti-aflatoxigenic activity. Results The droplet size of Saffron flowers was finer (121.64 ± 2.18 nm) than yarrow flowers (151.21 ± 1.12 nm). The Zeta potential measurements of the yarrow flower (-16.31 ± 2.54 mV) and the saffron flower (-18.55 ± 2.31 mV) both showed high stability, along with low PDI values (0.34–0.41). The nanoemulsion of yarrow flower revealed 51 compounds using gas chromatography-mass spectrometry (GCMS), with hexanal (16.25%), β-Pinene (7.41%), β-Myrcene (5.24%), D-Limonene (5.58%) and Caryophyllene (4.38%) being the most prevalent. Additionally, 31 compounds were detected in the saffron nanoemulsion, with D-limonene (4.89%), isophorone (12.29%), 4-oxy isophorone (8.19%), and safranal (44.84%) being the most abundant. Compared to the nanoemulsion of the yarrow flower, the saffron nanoemulsion had good antibacterial and antifungal activity. Saffron nanoemulsion inhibited total fungal growth by 69.64–71.90% in a simulated liquid medium and demonstrated the most significant decrease in aflatoxin production. Infected strawberry fruits coated with nanoemulsion extracts exhibited high antimicrobial activity in the form of saffron flower and yarrow flower extract nanoemulsions, which inhibited and/or controlled the growth of Aspergillus fungi. Due to this inhibition, the lag phase was noticeably prolonged, the cell load decreased, and the stability time increased. Conclusion This study will contribute to expanding the theoretical research and utilization of nanoemulsions as green protective agents in agricultural and food industries for a promising protection from the invasion of some pathogenic bacteria and fungi.