Green synthesis of gold nanoparticles from the extract of Crocus sativus to study the effect of antidepressant in adolescence and to observe its aggressive and impulsive behavior in rat models.

• Eco-friendly Gold NPs was prepared using Crocus Sativus extract. • Novel combination of this NPs provide biocompatibility and anti-oxidant efficacy. • Biocompatible Nanoparticle provides effective anti-inflammatory action by Rat model. • These materials could be used as effective therapeutic agent for Anti-depression action. Depression is a unipolar condition caused by a brain monoamine deficit that affects emotion, thinking, and function. Depression treatments involve limitations such poor absorption, a delay in therapeutic efficacy, unwanted side effects, and high-dose toxicity. A simple, affordable, clean, non-toxic, and environmentally friendly nanoparticle manufacturing method was used to fix this. This research created C.sativus gold nanoparticles (CS-AuNPs) from Crocus sativus extract and auric chloride (HAuCl 4). CS-AuNPs was spherical, 11–20 nm in size, and 550 nm absorbent after UV–vis, XRD, FTIR, SEM, and TEM analysis. Saffron action is increased using gold nanoparticles, a non-toxic drug delivery vehicle. Crocins, crocetin, and safranal are important antidepressants. Crocus sativus has been shown to effectively reduce proinflammatory cytokines mRNA expression (IL-1β, IL-6, and TNF-α) and inhibit NF-κB activation. With an IC 50 of 256 μg/ml for DPPH, IC 50 of 300 μg/ml for NBT assay and 90 % cell survival in cytotoxicity tests, this chemical is safe and effective as an antidepressant. The new study showed that C.sativus has therapeutic promise and that converting it from extract to nanoparticles will benefit depression treatment. [ABSTRACT FROM AUTHOR]