161 results on '"Cristina, Januário"'
Search Results
2. Vitamin D metabolism and extraskeletal outcomes: an update
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Isabela Cristina Januário Silva and Marise Lazaretti-Castro
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Vitamin D ,randomized controlled trial ,vitamin D/metabolism ,vitamin D/physiology ,vitamin D/therapeutic use ,Medicine ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
ABSTRACT Vitamin D deficiency is a general health problem affecting individuals at all stages of life and on different continents. The musculoskeletal effects of vitamin D are well known. Its deficiency causes rickets, osteomalacia, and secondary hyperparathyroidism and increases the risk of fractures. Clinical and experimental evidence suggests that vitamin D performs multiple extraskeletal functions. Several tissues unrelated to calcium and phosphate metabolism express vitamin D receptor (VDR) and are directly or indirectly influenced by 1,25(OH)2D (calcitriol). Some also express the enzyme 1 alpha-hydroxylase (CYP27B1) and produce 1,25(OH)2D, inducing autocrine or paracrine effects. Among the pleiotropic effects of vitamin D are the regulation of cell proliferation and differentiation, hormone secretion, and immune function. In this review, we outline vitamin D physiology and the outcomes of recent large RCTs on its potential extraskeletal effects. Those studies exhibit a need for continued clinical analysis to elucidate whether vitamin D status can influence extraskeletal health. Longer longitudinal follow-up and standardized assays are crucial to better assess potential outcomes.
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- 2022
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3. Cerebellar morphometric and spectroscopic biomarkers for Machado-Joseph Disease
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Catarina Oliveira Miranda, Rui Jorge Nobre, Vitor Hugo Paiva, João Valente Duarte, João Castelhano, Lorena Itatí Petrella, José Sereno, Magda Santana, Sónia Afonso, Cristina Januário, Miguel Castelo-Branco, and Luís Pereira de Almeida
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Machado-Joseph disease (MJD) ,Spinocerebellar ataxia type 3 (SCA3) ,Magnetic resonance imaging (MRI) ,Proton magnetic resonance spectroscopy (1H-MRS) biomarkers ,Motor performance ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Machado-Joseph disease (MJD) or Spinocerebellar ataxia type 3 (SCA3) is the most common form of dominant SCA worldwide. Magnetic Resonance Imaging (MRI) and Proton Magnetic Resonance Spectroscopy (1H-MRS) provide promising non-invasive diagnostic and follow-up tools, also serving to evaluate therapies efficacy. However, pre-clinical studies showing relationship between MRI-MRS based biomarkers and functional performance are missing, which hampers an efficient clinical translation of therapeutics. This study assessed motor behaviour, neurochemical profiles, and morphometry of the cerebellum of MJD transgenic mice and patients aiming at establishing magnetic-resonance-based biomarkers. 1H-MRS and structural MRI measurements of MJD transgenic mice were performed with a 9.4 Tesla scanner, correlated with motor performance on rotarod and compared with data collected from human patients. We found decreased cerebellar white and grey matter and enlargement of the fourth ventricle in both MJD mice and human patients as compared to controls. N-acetylaspartate (NAA), NAA + N-acetylaspartylglutamate (NAA + NAAG), Glutamate, and Taurine, were significantly decreased in MJD mouse cerebellum regardless of age, whereas myo-Inositol (Ins) was increased at early time-points. Lower neurochemical ratios levels (NAA/Ins and NAA/total Choline), previously correlated with worse clinical status in SCAs, were also observed in MJD mice cerebella. NAA, NAA + NAAG, Glutamate, and Taurine were also positively correlated with MJD mice motor performance. Importantly, these 1H-MRS results were largely analogous to those found for MJD in human studies and in our pilot data in human patients. We have established a magnetic resonance-based biomarker approach to monitor novel therapies in preclinical studies and human clinical trials.
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- 2022
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4. Defective mitochondria‐lysosomal axis enhances the release of extracellular vesicles containing mitochondrial DNA and proteins in Huntington's disease
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Margarida Beatriz, Rita Vilaça, Sandra I. Anjo, Bruno Manadas, Cristina Januário, A. Cristina Rego, and Carla Lopes
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autophagy ,extracellular vesicles ,Huntington's disease ,mitochondrial DNA ,mitochondrial dysfunction ,neuronal‐derived extracellular vesicles ,Cytology ,QH573-671 - Abstract
Abstract Mitochondrial and autophagy dysfunction are mechanisms proposed to be involved in the pathogenesis of several neurodegenerative diseases. Huntington's disease (HD) is a progressive neurodegenerative disorder associated with mutant Huntingtin‐induced abnormalities in neuronal mitochondrial dynamics and quality control. Former studies suggest that the removal of defective mitochondria may be compromised in HD. Mitochondrial quality control (MQC) is a complex, well‐orchestrated pathway that can be compromised through mitophagy dysregulation or impairment in the mitochondria‐lysosomal axis. Another mitochondrial stress response is the generation of mitochondrial‐derived vesicles that fuse with the endolysosomal system and form multivesicular bodies that are extruded from cells as extracellular vesicles (EVs). In this work, we aimed to study the presence of mitochondrial components in human EVs and the relation to the dysfunction of both mitochondria and the autophagy pathway. We comprehensively characterized the mitochondrial and autophagy alterations in premanifest and manifest HD carriers and performed a proteomic and genomic EVs profile. We observed that manifest HD patients exhibit mitochondrial and autophagy impairment associated with enhanced EVs release. Furthermore, we detected mitochondrial DNA and proteins in EVs released by HD cells and in neuronal‐derived EVs including VDAC‐1 and alpha and beta subunits of ATP synthase F1. HD‐extracellular vesicles transport higher levels of mitochondrial genetic material in manifest HD patients, suggesting an alternative pathway for the secretion of reactive mitochondrial components. This study provides a novel framework connecting EVs enhanced release of mitochondrial components to mitochondrial and lysosomal dysfunction in HD.
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- 2022
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5. Mitochondrial and redox modifications in early stages of Huntington's disease
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Carla Lopes, I. Luísa Ferreira, Carina Maranga, Margarida Beatriz, Sandra I. Mota, José Sereno, João Castelhano, Antero Abrunhosa, Francisco Oliveira, Maura De Rosa, Michael Hayden, Mário N. Laço, Cristina Januário, Miguel Castelo Branco, and A. Cristina Rego
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64Cu]-ATSM PET ,Human skin fibroblasts ,YAC128 mice ,Reactive oxygen species ,Mitochondrial bioenergetics ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Deficits in mitochondrial function and redox deregulation have been attributed to Huntington's disease (HD), a genetic neurodegenerative disorder largely affecting the striatum. However, whether these changes occur in early stages of the disease and can be detected in vivo is still unclear. In the present study, we analysed changes in mitochondrial function and production of reactive oxygen species (ROS) at early stages and with disease progression. Studies were performed in vivo in human brain by PET using [64Cu]-ATSM and ex vivo in human skin fibroblasts of premanifest and prodromal (Pre-M) and manifest HD carriers. In vivo brain [64Cu]-ATSM PET in YAC128 transgenic mouse and striatal and cortical isolated mitochondria were assessed at presymptomatic (3 month-old, mo) and symptomatic (6–12 mo) stages. Pre-M HD carriers exhibited enhanced whole-brain (with exception of caudate) [64Cu]-ATSM labelling, correlating with CAG repeat number. Fibroblasts from Pre-M showed enhanced basal and maximal respiration, proton leak and increased hydrogen peroxide (H2O2) levels, later progressing in manifest HD. Mitochondria from fibroblasts of Pre-M HD carriers also showed reduced circularity, while higher number of mitochondrial DNA copies correlated with maximal respiratory capacity. In vivo animal PET analysis showed increased accumulation of [64Cu]-ATSM in YAC128 mouse striatum. YAC128 mouse (at 3 months) striatal isolated mitochondria exhibited a rise in basal and maximal mitochondrial respiration and in ATP production, and increased complex II and III activities. YAC128 mouse striatal mitochondria also showed enhanced mitochondrial H2O2 levels and circularity, revealed by brain ultrastructure analysis, and defects in Ca2+ handling, supporting increased striatal susceptibility. Data demonstrate both human and mouse mitochondrial overactivity and altered morphology at early HD stages, facilitating redox unbalance, the latter progressing with manifest disease.
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- 2022
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6. Controlling Infectious Diseases: The Decisive Phase Effect on a Seasonal Vaccination Strategy.
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Jorge Duarte, Cristina Januário, Nuno Martins, Jesús M. Seoane, and Miguel A. F. Sanjuán
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- 2021
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7. A different vision of translational research in biomarker discovery: a pilot study on circulatory mitochondrial proteins as Parkinson’s disease potential biomarkers
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Sandra I. Anjo, Patrícia Valério dos Santos, Luiza Rosado, Graça Baltazar, Inês Baldeiras, Diana Pires, Andreia Gomes, Cristina Januário, Miguel Castelo-Branco, Mário Grãos, and Bruno Manadas
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Mitochondrial-related proteins ,SWATH-MS ,Parkinson’s disease ,Biomarker discovery ,Blood-biomarker ,Secretomes ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Background The identification of circulating biomarkers that closely correlate with Parkinson’s Disease (PD) has failed several times in the past. Nevertheless, in this pilot study, a translational approach was conducted, allowing the evaluation of the plasma levels of two mitochondrial-related proteins, whose combination leads to a robust model with potential diagnostic value to discriminate the PD patients from matched controls. Methods The proposed translational approach was initiated by the analysis of secretomes from cells cultured under control or well-defined oxidative stress conditions, followed by the identification of proteins related to PD pathologic mechanisms that were altered between the two states. This pipeline was further translated into the analysis of undepleted plasma samples from 28 control and 31 PD patients. Results From the secretome analysis, several mitochondria-related proteins were found to be differentially released between control and stress conditions and to be able to distinguish the two secretomes. Similarly, two mitochondrial-related proteins were found to be significantly changed in a PD cohort compared to matched controls. Moreover, a linear discriminant model with potential diagnostic value to discriminate PD patients was obtained using the combination of these two proteins. Both proteins are associated with apoptotic mitochondrial changes, which may correspond to potential indicators of cell death. Moreover, one of these proteins, the VPS35 protein, was reported in plasma for the first time, and its quantification was only possible due to its previous identification in the secretome analysis. Conclusions In this work, an adaptation of a translational pipeline for biomarker selection was presented and transposed to neurological diseases, in the present case Parkinson’s Disease. The novelty and success of this pilot study may arise from the combination of: i) a translational research pipeline, where plasma samples are interrogated using knowledge previously obtained from the evaluation of cells’ secretome under oxidative stress; ii) the combined used of statistical analysis and an informed selection of candidates based on their link with relevant disease mechanisms, and iii) the use of SWATH-MS, an untargeted MS method that allows a complete record of the analyzed samples and a targeted data extraction of the quantitative values of proteins previously identified.
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- 2020
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8. The effect of impulsivity and inhibitory control deficits in the saccadic behavior of premanifest Huntington’s disease individuals
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Filipa Júlio, Gina Caetano, Cristina Januário, and Miguel Castelo-Branco
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Huntington’s disease ,Cognition ,Oculomotor function ,Inhibitory control ,Impulsivity ,Medicine - Abstract
Abstract Background This study aims to test response inhibition in premanifest Huntington’s disease individuals (Pre-HD), in the context of a saccadic paradigm with working memory demands and fronto-executive load as a way to measure inhibitory control deficits and impulsive behavior in Huntington’s disease (HD). Methods The oculomotor function of 15 Pre-HD and 22 Control individuals was assessed using an experimental paradigm comprising four horizontal saccadic tasks: prosaccade (PS), antisaccade (AS), 1- or 2-back memory prosaccade (MPS), and 1- or 2-back memory antisaccade (MAS). Success rate, latency, directional and timing errors were calculated for each task. A comprehensive battery of neuropsychological tests was also used to assess the overall cognitive functioning of study participants. Statistical correlations between oculomotor, clinical and cognitive measures were computed for the Pre-HD group. Results Pre-HD participants showed reduced success rate in the AS task, increased direction errors in the AS and MAS tasks and decreased latency in the MAS task when compared to Controls, despite presenting similar executive and memory scores in the conventional neuropsychological tests applied. Significant associations were identified between specific AS and MAS parameters and disease-related measures, cognitive skills and other oculomotor results of Pre-HD participants. Conclusions Our results show that oculomotor performance in premanifest Huntington’s disease deteriorates once inhibitory control, working memory and/or fronto-executive load are added to the task. A more automatic pattern of performance, including a faster response time and directionally erroneous eye movements were detected in the oculomotor behavior of the Pre-HD group—these alterations were significantly correlated with disease stage and cognitive status. Our saccadic paradigm was able to capture impulsivity and inhibitory control deficits in a group of Pre-HD individuals on average far from symptom onset, thus holding the potential to identify the earliest disease-related changes.
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- 2019
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9. On the Dynamical Complexity of a Seasonally Forced Discrete SIR Epidemic Model with a Constant Vaccination Strategy.
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Jalil Rashidinia, Mehri Sajjadian, Jorge Duarte, Cristina Januário, and Nuno Martins
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- 2018
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10. Optimal homotopy analysis of a chaotic HIV-1 model incorporating AIDS-related cancer cells.
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Jorge Duarte, Cristina Januário, Nuno Martins, Carlos Correia Ramos, Carla Rodrigues, and Josep Sardanyés
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- 2018
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11. Adverse events related to medication administration in a Taquari Valley hospital
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Kellen Cristina Januário Benedetti Alves, Paula Michele Lohmann, Arlete Eli Kunz da Costa, and Fernanda dos Santos
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erros de medicação. segurança do paciente. sistemas de notificação de reações adversas a medicamentos. ,Medicine ,Internal medicine ,RC31-1245 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Background and Objectives: The medication process in the hospital environment is one of the most important activities to ensure efficacy in the patient's therapy. The objective of this study was to know the perception of the nursing team about the occurrence of adverse events related to drug administration in a hospital in Vale do Taquari, Rio Grande do Sul. Methods: This is a descriptive exploratory study with a qualitative approach-quantitative. Data were collected through the application of a questionnaire to nursing professionals, constituting a sample of fifty-eight interviewees. The analysis was made through descriptive statistics. Results: Of the participants, 96.6% knew the steps to avoid medication errors, and 79.3% had already made some mistake in their professional practices in the institution. The most frequent error was the erroneous administration dose of 43.5%, with 97.3% of the professionals already perceived some error in the medication process and 30% considered the work overload to be an aggravating factor for the occurrence of errors. Regarding the professional's behavior regarding the error, 80.4% communicated verbally to the supervising professional and as a consequence received verbal guidance 78.3% of the time. As for feelings about the error 36.8% revealed to feel fear of harming the patient. Conclusion: These findings point to serious potential errors that impair patient safety; however, it is noted that verbal guidelines are offered avoiding punishment, but the incentive for reporting the error is precarious, making it difficult for the team to identify potential errors and implement barriers to avoid them.
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- 2020
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12. Reliability and Validity of 39-Item Parkinson’s Disease Questionnaire and Parkinson’s Disease Quality of Life Questionnaire
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Joana Jesus-Ribeiro, Elsa Vieira, Pedro Ferreira, Cristina Januário, and António Freire
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Parkinson Disease ,Quality of Life ,Surveys and Questionnaires ,Medicine ,Medicine (General) ,R5-920 - Abstract
Introduction: Parkinson’s disease has a significant impact in quality of life, which can be assessed with 39-item Parkinson’s Disease Questionnaire and Parkinson’s Disease Quality of Life Questionnaire. This study aimed to evaluate the reliability and validity of these scales in Portuguese patients. Material and Methods: Reliability was assessed through internal consistency (Cronbach’s alpha) and reproducibility (intraclass correlation coefficient). Regarding construct validity, we performed one-way analysis of variance across different groups according to modified Hoehn and Yahr scale. For criterion validity, we compared both scales with each other and with the Short Form 36-item Health Survey. Results: In a total of 100 patients with Parkinson’s disease, Cronbach’s alpha ranged for 39-item Parkinson’s Disease Questionnaire between 0.66 - 0.98, and for Parkinson’s Disease Quality of Life Questionnaire, between 0.78 - 0.98. Intraclass correlation coefficient for 39-item Parkinson’s Disease Questionnaire ranged between 0.49 - 0.96, and for Parkinson’s Disease Quality of Life Questionnaire, ranged between 0.65 - 0.96. Both scales showed, in general, capacity to discriminate differences among patients in the different stages of disease. The scales presented moderate to strong magnitude correlations with some Short Form 36-item Health Survey domains. Discussion: Cronbach’s alpha coefficients for most domains were satisfactory. Overall, it has been demonstrated good reproducibility, as well as construct and criterion validity. Conclusion: The Portuguese versions of both scales showed to be valid and reliable.
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- 2017
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13. A Novel Ecological Approach Reveals Early Executive Function Impairments in Huntington’s Disease
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Filipa Júlio, Maria J. Ribeiro, Miguel Patrício, Alexandre Malhão, Fábio Pedrosa, Hélio Gonçalves, Marco Simões, Marieke van Asselen, Mário R. Simões, Miguel Castelo-Branco, and Cristina Januário
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Huntington’s disease ,executive functions ,activities of daily living ,ecological validity ,virtual reality ,Psychology ,BF1-990 - Abstract
Introduction: Impairments in executive functions are common in neurogenetic disorders such as Huntington’s disease (HD) and are thought to significantly influence the patient’s functional status. Reliable tools with higher ecological validity that can assess and predict the impact of executive dysfunction in daily-life performance are needed. This study aimed to develop and validate a novel non-immersive virtual reality task (“EcoKitchen”) created with the purpose of capturing cognitive and functional changes shown by HD carriers without clinical manifestations of the disease (Premanifest HD), in a more realistic setting.Materials and Methods: We designed a virtual reality task with three blocks of increasing executive load. The performance of three groups (Controls, CTRL; Premanifest HD individuals, HP; Early Manifest HD patients, HD) was compared in four main components of the study protocol: the EcoKitchen; a subjective (self-report) measure – “The Adults and Older Adults Functional Assessment Inventory (IAFAI)”; the “Behavioural Assessment of Dysexecutive Syndrome battery (BADS)”; and a conventional neuropsychological test battery. We also examined statistical associations between EcoKitchen and the other executive, functional and clinical measures used.Results: The HD group showed deficits in all the assessment methods used. In contrast, the HP group was only found to be impaired in the EcoKitchen task, particularly in the most cognitively demanding blocks, where they showed a higher number of errors compared to the CTRL group. Statistically significant correlations were identified between the EcoKitchen, measures of the other assessment tools, and HD clinical features.Discussion: The EcoKitchen task, developed as an ecological executive function assessment tool, was found to be sensitive to early deficits in this domain. Critically, in premanifest HD individuals, it identifies dysfunction prior to symptom onset. Further it adds a potential tool for diagnosis and management of the patients’ real-life problems.
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- 2019
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14. Retinal texture biomarkers may help to discriminate between Alzheimer's, Parkinson's, and healthy controls.
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Ana Nunes, Gilberto Silva, Cristina Duque, Cristina Januário, Isabel Santana, António Francisco Ambrósio, Miguel Castelo-Branco, and Rui Bernardes
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Medicine ,Science - Abstract
A top priority in biomarker development for Alzheimer's disease (AD) and Parkinson's disease (PD) is the focus on early diagnosis, where the use of the retina is a promising avenue of research. We computed fundus images from optical coherence tomography (OCT) data and analysed the structural arrangement of the retinal tissue using texture metrics. We built clinical class classification models to distinguish between healthy controls (HC), AD, and PD, using machine learning (support vector machines). Median sensitivity is 88.7%, 79.5% and 77.8%, for HC, AD, and PD eyes, respectively. When the same subject has the same classification for both eyes, 94.4% (median) of the classifications are correct. A significant amount of information discriminating between multiple neurodegenerative states is conveyed by OCT imaging of the human retina, even when differences in thickness are not yet present. This technique may allow for simultaneously diagnosing Alzheimer's and Parkinson's diseases.
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- 2019
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15. Impulsivity across reactive, proactive and cognitive domains in Parkinson's disease on dopaminergic medication: Evidence for multiple domain impairment.
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Nádia Canário, Mário Sousa, Fradique Moreira, Isabel Catarina Duarte, Francisco Oliveira, Cristina Januário, and Miguel Castelo-Branco
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Medicine ,Science - Abstract
Impulse control disorders (ICD) may occur in Parkinson's disease (PD) although it remains to be understood if such deficits may occur even in the absence of a formal ICD diagnosis. Moreover, studies addressing simultaneously distinct neurobehavioral domains, such as cognitive, proactive and reactive motor impulsivity, are still lacking. Here, we aimed to investigate if reactive, proactive and cognitive impulsivity involving risk taking are concomitantly affected in medicated PD patients, and whether deficits were dependent on response strategies, such as speed accuracy tradeoffs, or the proportion of omission vs. commission errors. We assessed three different impulsivity domains in a sample of 21 PD patients and 13 matched controls. We found impaired impulsivity in both reactive (p = 0.042) and cognitive domains (p = 0.015) for the PD patients, irrespective of response strategy. For the latter, effect sizes were larger for the actions related with reward processing (p = 0.017, dCohen = 0.9). In the proactive impulsivity task, PD patients showed significantly increased number of omissions (p = 0.041), a response strategy which was associated with preserved number of commission errors. Moreover, the number of premature and proactive response errors were correlated with disease stage. Our findings suggest that PD ON medication is characterized compared to healthy controls by impairment across several impulsivity domains, which is moderated in the proactive domain by the response strategy.
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- 2019
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16. Characterizing Parkinson's Disease Speech by Acoustic and Phonetic Features.
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Jorge Proença, Arlindo Veiga, Sara Candeias, João Lemos, Cristina Januário, and Fernando Perdigão
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- 2014
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17. Activation of effector immune cells promotes tumor stochastic extinction: A homotopy analysis approach.
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Josep Sardanyés, Carla Rodrigues, Cristina Januário, Nuno Martins, Gabriel Gil-Gómez, and Jorge Duarte
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- 2015
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18. Tremor modulations across periods with and without voluntary motion and limb load task demands using movement quantification.
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Paula Faria, Miguel Patrício, Gregor Philipiak, Francisco Caramelo, Cristina Januário, Antonio Freire, and Miguel Castelo-Branco
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- 2013
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19. Tourette's syndrome and associated disorders: a systematic review
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Bárbara R. Ferreira, J. L. Pio-Abreu, and Cristina Januário
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Perturbação de déficit de atenção com hiperatividade ,comorbidades ,perturbação obsessivo-compulsiva ,tiques ,síndrome de Tourette ,Psychiatry ,RC435-571 - Abstract
Objective: To compile data on Tourette's syndrome (TS), tics and associated disorders.Methods: A systematic review of the literature was conducted using the 5S levels of organization of healthcare research evidence (systems, summaries, synopses, syntheses, studies), based on the model described by Haynes. The search keywords were Tourette, tics and comorbidity, which were cross-referenced. Studies provided by publishers and articles being processed on July 31, 2013, were also included.Results: Of all studies retrieved during the search, 64 were selected because they analyzed the epidemiology, clinical features and etiopathogenesis of TS and its comorbidities. TS is classified as a hyperkinetic movement disorder, and at least 90% of the patients have neuropsychiatric comorbidities, of which attention deficit hyperactivity and obsessive-compulsive disorders are the most common. The syndrome is clinically heterogeneous and has been associated with a dysfunction of cortico-striatal-thalamic-cortical circuits involving various neurotransmitters. Although its genetic etiology has been widely studied, other factors may be important to understand this syndrome and its associated disorders.Conclusions: TS is a neurodevelopmental disorder that results from the impact of stress factors on a vulnerable biological substrate during the critical periods of neurodevelopment. The study of TS and its comorbidities may contribute, at different levels, to the understanding of several neuropsychiatric disorders of clinical and therapeutic relevance.
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- 2014
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20. Homotopy analysis of explicit solutions in a chronic hepatitis C virus model
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Nuno Martins, Cristina Januário, and Jorge Duarte
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Chronic hepatitis ,Applied Mathematics ,Homotopy ,Virology ,Virus ,Mathematics - Published
- 2021
21. Static and Dynamic Ocular Motor Abnormalities as Potential Biomarkers in Spinocerebellar Ataxia Type 3
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Ana Novo, Cristina Januário, Cristina Duque, Inês Cunha, João Lemos, João Castelhano, and Joana Ribeiro
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medicine.medical_specialty ,Neurology ,Ataxia ,genetic structures ,business.industry ,05 social sciences ,Nystagmus ,Optokinetic reflex ,medicine.disease ,Dysmetric saccades ,050105 experimental psychology ,03 medical and health sciences ,0302 clinical medicine ,Ophthalmology ,medicine ,Reflex ,Spinocerebellar ataxia ,0501 psychology and cognitive sciences ,Neurology (clinical) ,medicine.symptom ,Strabismus ,business ,030217 neurology & neurosurgery - Abstract
While dynamic ocular motor abnormalities (e.g., gaze-evoked nystagmus (GEN), low optokinetic nystagmus (OKN), pursuit and vestibulo-ocular reflex (VOR) gains, and dysmetric saccades) have been shown to be potential biomarkers in spinocerebellar ataxia type 3 (SCA3), the value of static abnormalities (e.g., convergent [esodeviation] and divergent strabismus [exodeviation]) is unknown. Moreover, studies on dynamic abnormalities in SCA3 usually do not take into account the existence of potential abduction-adduction asymmetries in patients with degenerative ataxia. Thirty-eight patients with genetically confirmed SCA3 (24 females; mean age ± SD, 49.8± 12.2 years) and 22 healthy controls (12 females, p = 0.589; mean age ± SD, 50.7± 12.5 years, p = 0.651) underwent clinical and video-oculographic assessment. A p value
- Published
- 2020
22. Tau and neurofilament light-chain as fluid biomarkers in spinocerebellar ataxia type 3
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Hector Garcia‐Moreno, Mercedes Prudencio, Gilbert Thomas‐Black, Nita Solanky, Karen R. Jansen‐West, Rana Hanna AL‐Shaikh, Amanda Heslegrave, Henrik Zetterberg, Magda M. Santana, Luis Pereira de Almeida, Ana Vasconcelos‐Ferreira, Cristina Januário, Jon Infante, Jennifer Faber, Thomas Klockgether, Kathrin Reetz, Mafalda Raposo, Ana F. Ferreira, Manuela Lima, Ludger Schöls, Matthis Synofzik, Jeannette Hübener‐Schmid, Andreas Puschmann, Sorina Gorcenco, Zbigniew K. Wszolek, Leonard Petrucelli, Paola Giunti, and Universidad de Cantabria
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blood [tau Proteins] ,Heterozygote ,blood [Neurofilament Proteins] ,Mice, Transgenic ,tau Proteins ,blood [Machado-Joseph Disease] ,Mice ,Neurofilament Proteins ,genetics [Machado-Joseph Disease] ,Cerebellum ,Animals ,Humans ,ddc:610 ,blood [Biomarkers] ,Neurofilaments ,Machado-Joseph Disease ,cerebrospinal fluid [Neurofilament Proteins] ,Neurofilaments, Tau ,Spinocerebellar ataxias ,genetics [tau Proteins] ,cerebrospinal fluid [Biomarkers] ,cerebrospinal fluid [tau Proteins] ,Neurology ,cerebrospinal fluid [Machado-Joseph Disease] ,Neurology (clinical) ,Tau ,Biomarkers ,chemistry [Cerebellum] - Abstract
Background and purpose: Clinical trials in spinocerebellar ataxia type 3 (SCA3) will require biomarkers for use as outcome measures. Methods: To evaluate total tau (t-tau), glial fibrillary acidic protein (GFAP), ubiquitin carboxy-terminal hydrolase L1 (UCHL1) and neurofilament light-chain (NfL) as fluid biomarkers in SCA3, ATXN3 mutation carriers (n = 143) and controls (n = 172) were clinically assessed, and the plasma concentrations of the four proteins were analysed on the Simoa HD-1 platform. Eleven ATXN3 mutation carrier cerebrospinal fluid samples were analysed for t-tau and phosphorylated tau (p-tau181 ). A transgenic SCA3 mouse model (MJDTg) was used to measure cerebellar t-tau levels. Results: Plasma t-tau levels were higher in mutation carriers below the age of 50 compared to controls, and the Inventory of Non-Ataxia Signs was associated with t-tau in ataxic patients (p = 0.004). Pre-ataxic carriers showed higher cerebrospinal fluid t-tau and p-tau181 concentrations compared to ataxic patients (p = 0.025 and p = 0.014, respectively). Cerebellar t-tau was elevated in MJDTg mice compared to wild-type (p = 0.033) only in the early stages of the disease. GFAP and UCHL1 did not show higher levels in mutation carriers compared to controls. Plasma NfL concentrations were higher in mutation carriers compared to controls, and differences were greater for younger carriers. The Scale for the Assessment and Rating of Ataxia was the strongest predictor of NfL in ataxic patients (p < 0.001). Conclusion: Our results suggest that tau might be a marker of early disease stages in SCA3. NfL can discriminate mutation carriers from controls and is associated with different clinical variables. Longitudinal studies are required to confirm their potential role as biomarkers in clinical trials. Federal Ministry of Education and Research, Grant/Award Number: 01ED1602A/B; The Netherlands Organisation for Health Research and Development; Fundação para a Ciência e a Tecnologia (FCT); Medical Research Council, Grant/Award Number: MR/N028767/1; Department of Health’s National Institute for Health Research Biomedical Research Centre’s funding scheme; National Institute for Health Research University College London Hospitals Biomedical Research Centre UCLH; Swedish Research Council, Grant/Award Number: 2018-02532; European Research Council, Grant/Award Number: 681712; Swedish State Support for Clinical Research, Grant/Award Number: ALFGBG-720931; Alzheimer Drug Discovery Foundation (ADDF), Grant/Award Number: 201809-2016862; National Ataxia Foundation; Hertie Academy for Clinical Neuroscience; German Federal Ministry of Education and Research, Grant/Award Number: 01GQ1402 and 01DN18022; German Research Foundation, Grant/Award Number: IRTG 2150 and ZUK32/1; NIH/National Institute of Neurological Disorder and Stroke, Grant/Award Number: P01NS084974, R01NS088689, R35NS097273 and R21NS084528; Department of Defense, Grant/Award Number: ALSRP AL130125; Mayo Clinic Foundation; Amyotrophic Lateral Sclerosis Association; Robert Packard Center for ALS Research at Johns Hopkins; Target ALS Foundation; Mayo Clinic Center for Regenerative Medicine; Mayo Clinic Neuroscience Focused Research Team; Albertson Parkinson’s Research Foundation; European Union’s Horizon 2020 research and innovation programme, Grant/Award Number: 643417; CureSCA3; Regional Fund for Science and Technology (FRCT), PRO-SCIENTIA program, Azores Government; SCA-network, Sweden; Region Skåne, Sweden; Sol Goldman Charitable Trust; Donald G and Jodi P Heeringa Family; The Haworth Family Professorship in Neurodegenerative Diseases fund; Alzheimer Forschung Initiative e.V., Grant/Award Number: AFI13812 and NL-18002CB; Fundo Social Europeu (FSE); ALF, Sweden
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- 2022
23. Defective mitochondrial-lysosomal axis promotes extracellular vesicles release of mitochondrial components in Huntington’s Disease
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Margarida Beatriz, Rita Vilaça, Sandra I. Anjo, Bruno Manadas, Cristina Januário, A. Cristina Rego, and Carla Lopes
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Mitochondrial and autophagy dysfunction are mechanisms proposed to be involved in the pathogenesis of several neurodegenerative diseases. Huntington’s disease (HD) is a progressive neurodegenerative disorder associated with mutant Huntingtin-induced abnormalities in neuronal mitochondrial dynamics and quality control. Former studies suggest that the removal of defective mitochondria may be compromised in HD. The mitochondrial quality control is a complex, well-orchestrated pathway that can be compromised through mitophagy dysregulation or impairment in the mitochondrial-lysosomal axis. Another mitochondrial stress response is the generation of mitochondrial-derived vesicles that fuse with the endolysosomal system and form multivesicular bodies that are extruded from cells as extracellular vesicles (EVs).In this study, we comprehensively characterized the mitochondrial and autophagy alterations in premanifest and manifest HD patients and performed a proteomic and genomic EVs profile. We observed that manifest HD patients exhibit mitochondrial and autophagy impairment associated with enhanced EVs release. Further, we detected mitochondrial components in EVs released by HD cells and in neuron-derived EVs. The EV-associated mtDNA copies were elevated in manifest HD patients suggesting to be an alternative pathway for secretion of reactive mitochondrial components. This study provides a novel framework connecting EVs enhanced release of mitochondrial components to mitochondrial and lysosomal dysfunction in HD.
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- 2022
24. Measuring and Controlling the Chaotic Motion of Profits.
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Cristina Januário, Clara Grácio, Diana A. Mendes, and Jorge Duarte
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- 2009
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25. Cognition, function and awareness of disease impact in early Parkinson’s and Huntington’s disease
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Miguel Castelo-Branco, Cristina Januário, Marieke van Asselen, Maria J. Ribeiro, Filipa Júlio, Mário R. Simões, Mário Sousa, and Ana Morgadinho
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Dysexecutive syndrome ,medicine.medical_specialty ,Rehabilitation ,Activities of daily living ,business.industry ,medicine.medical_treatment ,Parkinson Disease ,Cognition ,Disease ,Neuropsychological Tests ,medicine.disease ,Huntington Disease ,Physical medicine and rehabilitation ,Huntington's disease ,Intervention (counseling) ,Basal ganglia ,Humans ,Medicine ,Cognition Disorders ,business ,Aged - Abstract
Patients with Parkinson’s and Huntington’s Disease (PD and HD) present impairments in cognitively challenging everyday activities. This study contrasts these two basal ganglia disorders on the ability to perform daily life- like tasks and their level of awareness regarding the disease impact on function. 19 controls, 10 early-onset PD, 20 early stage PD, and 15 early manifest HD patients were compared in the “EcoKitchen,” a virtual reality task with increasing executive load, the “Behavioural Assessment of Dysexecutive Syndrome battery – BADS,” and “The Adults and Older Adults Functional Assessment Inventory – IAFAI,” a self-report functional questionnaire. The EcoKitchen clinical correlates were investigated. All clinical groups presented slower EcoKitchen performance than controls, however, only HD patients showed decreased accuracy. HD and PD patients exhibited reduced BADS scores compared to the other study participants. Importantly, on the IAFAI, PD patients signalled more physically related incapacities and HD patients indicated more cognitively related incapacities. Accordingly, the EcoKitchen performance was significantly associated with PD motor symptom severity. Our findings suggest differential disease impact on cognition and function across PD and HD patients, with preserved awareness regarding disease- related functional sequelae. These observations have important implications for clinical management, research and rehabilitation.Implications for rehabilitationPatients with early stage Parkinson’s and Huntington’s disease have diagnosis-specific impairments in the performance of executively demanding everyday activities and, yet, show preserved awareness about the disease impact on their daily life.An active involvement of patients in the rehabilitation process should be encouraged, as their appraisal of the disease effects can help on practical decisions about meaningful targets for intervention, vocational choices, quality-of-life issues and/or specific everyday skills to boost.The EcoKitchen, a non-immersive virtual reality task, can detect and quantify early deficits in everyday-like tasks and is therefore a valuable tool for assessing the effects of rehabilitation strategies on the functional cognition of these patients.Rehabilitation efforts in the mild stages of Parkinson’s and Huntington’s disease should be aware of greater time needs from the patients in the performance of daily life tasks, target executive skills, and give a more prominent role to patients in symptoms report and management. Patients with early stage Parkinson’s and Huntington’s disease have diagnosis-specific impairments in the performance of executively demanding everyday activities and, yet, show preserved awareness about the disease impact on their daily life. An active involvement of patients in the rehabilitation process should be encouraged, as their appraisal of the disease effects can help on practical decisions about meaningful targets for intervention, vocational choices, quality-of-life issues and/or specific everyday skills to boost. The EcoKitchen, a non-immersive virtual reality task, can detect and quantify early deficits in everyday-like tasks and is therefore a valuable tool for assessing the effects of rehabilitation strategies on the functional cognition of these patients. Rehabilitation efforts in the mild stages of Parkinson’s and Huntington’s disease should be aware of greater time needs from the patients in the performance of daily life tasks, target executive skills, and give a more prominent role to patients in symptoms report and management.
- Published
- 2020
26. Utility of the Parkinson’s disease-Cognitive Rating Scale for the screening of global cognitive status in Huntington’s disease
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Caterina Mariotti, Giuseppe De Michele, Josef Priller, Veronica Mañanes, Frederic Sampedro, Pedro J. Garcia-Ruiz, Simone Migliore, Ferdinando Squitieri, Filipa Júlio, Angela Nuzzi, Jesus Ruiz-Idiago, Javier Pagonabarraga, Stefanie Kehrer, Asunción Martinez-Descals, Marina de Tommaso, Noelia Rodriguez, Jesús Pérez-Pérez, Cristina Januário, Anna Castaldo, Saul Martinez-Horta, Sandra Noguera, Natascia De Lucia, Andrea Horta-Barba, Jose Luis Lopez-Sendon, Marianna Delussi, Emilia J. Sitek, and Jaime Kulisevsky
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Adult ,Male ,medicine.medical_specialty ,Neurology ,Clinical Dementia Rating ,Context (language use) ,Neuropsychological Tests ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Physical medicine and rehabilitation ,Huntington's disease ,Rating scale ,medicine ,Humans ,Dementia ,Cognitive Dysfunction ,030212 general & internal medicine ,business.industry ,Neuropsychology ,Reproducibility of Results ,Cognition ,Middle Aged ,medicine.disease ,Huntington Disease ,Female ,Neurology (clinical) ,business ,030217 neurology & neurosurgery - Abstract
Cognitive impairment is an essential feature of Huntington’s disease (HD) and dementia is a predictable outcome in all patients. However, validated instruments to assess global cognitive performance in the field of HD are lacking. We aimed to explore the utility of the Parkinson’s disease-Cognitive Rating Scale (PD-CRS) for the screening of global cognition in HD. A multicenter cohort of 132 HD patients at different disease stages and 33 matched healthy controls were classified as having preserved cognition, mild cognitive impairment (HD-MCI) or dementia (HD-Dem) according to the Clinical Dementia Rating and Functional Independence Score. The PD-CRS and the Mini-Mental State Examination were administered. Receiver operating characteristic curve analysis was used to determine optimal cutoffs to differentiate patients according to their cognitive status. A PD-CRS cutoff score ≤ 81/82 was optimal to detect HD-MCI (sensitivity = 93%; specificity = 80%; area under the curve (AUC) = 0.940), and ≤ 63/64 was optimal to detect HD-Dem (sensitivity = 90%; specificity = 87%; AUC = 0.933). MMSE scores failed to show robust psychometric properties in this context. The PD-CRS is a valid and reliable instrument to assess global cognition in HD in routine clinical care and clinical trials.
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- 2020
27. Characterization of Lifestyle in Spinocerebellar Ataxia Type 3 and Association with Disease Severity
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Matthis Synofzik, Khalaf Bushara, Hector Garcia-Moreno, Luís Pereira de Almeida, Magda M. Santana, Jon Infante, Patrick Silva, Jeannette Hübener-Schmid, Bart P.C. van de Warrenburg, Kathrin Reetz, Jennifer Faber, Heike Jacobi, Cristina Januário, Andreas Thieme, Ludger Schöls, Holger Hengel, Nita Solanky, Ana F. Ferreira, Jeremy D. Schmahmann, Paola Giunti, Manuela Lima, Peter Martus, Chiadi U. Onyike, Thomas Klockgether, Jeroen J de Vries, and Universidad de Cantabria
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medicine.medical_specialty ,lifestyle ,congenital, hereditary, and neonatal diseases and abnormalities ,Movement disorders ,Medizin ,physical activity ,body mass index ,Disease ,Severity of Illness Index ,SCA3 ,Internal medicine ,medicine ,Spinocerebellar Ataxias ,Humans ,Prospective Studies ,ddc:610 ,Prospective cohort study ,Association (psychology) ,Life Style ,complications [Spinocerebellar Ataxias] ,business.industry ,alcohol ,Machado-Joseph Disease ,Disorders of movement Donders Center for Medical Neuroscience [Radboudumc 3] ,medicine.disease ,Neurology ,Spinocerebellar ataxia ,epidemiology [Spinocerebellar Ataxias] ,Neurology (clinical) ,medicine.symptom ,Age of onset ,business ,Body mass index ,Alcohol Abstinence - Abstract
Movement disorders 37(2), 405-410 (2022). doi:10.1002/mds.28844, Published by Wiley, New York, NY
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- 2022
28. The effect of impulsivity and inhibitory control deficits in the saccadic behavior of premanifest Huntington’s disease individuals
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Gina Caetano, Cristina Januário, Filipa Júlio, and Miguel Castelo-Branco
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Adult ,Male ,medicine.medical_specialty ,Impulsivity ,lcsh:Medicine ,Context (language use) ,Neuropsychological Tests ,Audiology ,050105 experimental psychology ,03 medical and health sciences ,0302 clinical medicine ,Cognition ,Huntington's disease ,Reaction Time ,medicine ,Humans ,Oculomotor function ,0501 psychology and cognitive sciences ,Pharmacology (medical) ,Inhibitory control ,Genetics (clinical) ,Working memory ,Research ,05 social sciences ,lcsh:R ,Neuropsychology ,Eye movement ,General Medicine ,Middle Aged ,medicine.disease ,Saccadic masking ,Huntington Disease ,Memory, Short-Term ,Female ,medicine.symptom ,Psychology ,030217 neurology & neurosurgery ,Huntington’s disease - Abstract
BackgroundThis study aims to test response inhibition in premanifest Huntington’s disease individuals (Pre-HD), in the context of a saccadic paradigm with working memory demands and fronto-executive load as a way to measure inhibitory control deficits and impulsive behavior in Huntington’s disease (HD).MethodsThe oculomotor function of 15 Pre-HD and 22 Control individuals was assessed using an experimental paradigm comprising four horizontal saccadic tasks: prosaccade (PS), antisaccade (AS), 1- or 2-back memory prosaccade (MPS), and 1- or 2-back memory antisaccade (MAS). Success rate, latency, directional and timing errors were calculated for each task. A comprehensive battery of neuropsychological tests was also used to assess the overall cognitive functioning of study participants. Statistical correlations between oculomotor, clinical and cognitive measures were computed for the Pre-HD group.ResultsPre-HD participants showed reduced success rate in the AS task, increased direction errors in the AS and MAS tasks and decreased latency in the MAS task when compared to Controls, despite presenting similar executive and memory scores in the conventional neuropsychological tests applied. Significant associations were identified between specific AS and MAS parameters and disease-related measures, cognitive skills and other oculomotor results of Pre-HD participants.ConclusionsOur results show that oculomotor performance in premanifest Huntington’s disease deteriorates once inhibitory control, working memory and/or fronto-executive load are added to the task. A more automatic pattern of performance, including a faster response time and directionally erroneous eye movements were detected in the oculomotor behavior of the Pre-HD group—these alterations were significantly correlated with disease stage and cognitive status. Our saccadic paradigm was able to capture impulsivity and inhibitory control deficits in a group of Pre-HD individuals on average far from symptom onset, thus holding the potential to identify the earliest disease-related changes.
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- 2019
29. A SIR forced model with interplays with the external world and periodic internal contact interplays
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Alberto d'Onofrio, Jorge Duarte, Cristina Januário, Nuno Martins, D'Onofrio, A., Duarte, J., Januario, C., and Martins, N.
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Infectious diseases modeling ,infectious diseases modeling ,Subharmonic resonance ,Chaos ,General Physics and Astronomy ,Seasonality ,SIR model ,Chao - Abstract
We investigated the behavior of a Susceptible-Infected-Recovered seasonally forced model for endemic childhood infectious diseases in the case where the target population is not isolated and, moreover, fast weekly fluctuations of the social contacts occur. We considered some key scenarios of interplay of Susceptible subjects with the external world, leading to subharmonic resonances and chaos. Our simulations suggest that the above–mentioned fast oscillations of the contact rate can cause the suppression/reduction of chaos and of subharmonic resonances. Thus, far from being filtered, they have an important role. If one considers an opposition of phase of the pattern of external infections w.r.t. the pattern of internal transmission rate, they result remarkably different from a scenario of synchrony. In most scenarios, the chaotic behavior is not associated to the phenomenon of the ‘atom–infectious’, i.e. the proportion of infectious is small but not unrealistic for large populations. published
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- 2022
30. Vitamin D metabolism and extraskeletal outcomes: an update
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Silva, Isabela Cristina Januário, primary and Lazaretti-Castro, Marise, additional
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- 2022
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31. No meio da travessia: Homenagem ao Prof. Luiz de Sousa Junior
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Edineide Jezine and Giovanna Cristina Januário Alves
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General Engineering - Published
- 2021
32. Automatic classification of idiopathic Parkinson's disease and atypical parkinsonian syndromes combining [11C]raclopride PET uptake and MRI grey matter morphometry
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Cristina Januário, Antero J. Abrunhosa, Ana Paula Moreira, Francisco P. M. Oliveira, Miguel Castelo-Branco, Fradique Moreira, and Ricardo Martins
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Raclopride ,medicine.diagnostic_test ,business.industry ,Dementia with Lewy bodies ,11C-Raclopride positron emission tomography ,Computer-aided diagnosis ,Parkinsonian syndromes ,machine learning ,magnetic resonance imaging ,multimodality imaging ,0206 medical engineering ,Biomedical Engineering ,Magnetic resonance imaging ,02 engineering and technology ,Grey matter ,medicine.disease ,020601 biomedical engineering ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,0302 clinical medicine ,medicine.anatomical_structure ,Atrophy ,Positron emission tomography ,Medicine ,Corticobasal degeneration ,business ,Nuclear medicine ,030217 neurology & neurosurgery ,medicine.drug - Abstract
Objective.To explore the viability of developing a computer-aided diagnostic system for Parkinsonian syndromes using dynamic [11C]raclopride positron emission tomography (PET) and T1-weighted magnetic resonance imaging (MRI) data.Approach.The biological heterogeneity of Parkinsonian syndromes renders their statistical classification a challenge. The unique combination of structural and molecular imaging data allowed different classifier designs to be tested. Datasets from dynamic [11C]raclopride PET and T1-weighted MRI scans were acquired from six groups of participants. There were healthy controls (CTRLn= 15), patients with Parkinson’s disease (PDn= 27), multiple system atrophy (MSAn= 8), corticobasal degeneration (CBDn= 6), and dementia with Lewy bodies (DLBn= 5). MSA, CBD, and DLB patients were classified into one category designated as atypical Parkinsonism (AP). The distribution volume ratio (DVR) kinetic parameters obtained from the PET data were used to quantify the reversible tracer binding to D2/D3 receptors in the subcortical regions of interest (ROI). The grey matter (GM) volumes obtained from the MRI data were used to quantify GM atrophy across cortical, subcortical, and cerebellar ROI.Results.The classifiers CTRL vs PD and CTRL vs AP achieved the highest balanced accuracy combining DVR and GM (DVR-GM) features (96.7%, 92.1%, respectively), followed by the classifiers designed with DVR features (93.3%, 88.8%, respectively), and GM features (69.6%, 86.1%, respectively). In contrast, the classifier PD vs AP showed the highest balanced accuracy (78.9%) using DVR features only. The integration of DVR-GM (77.9%) and GM features (72.7%) produced inferior performances. The classifier CTRL vs PD vs AP showed high weighted balanced accuracy when DVR (80.5%) or DVR-GM features (79.9%) were integrated. GM features revealed poorer performance (59.5%).Significance.This work was unique in its combination of structural and molecular imaging features in binary and triple category classifications. We were able to demonstrate improved binary classification of healthy/diseased status (concerning both PD and AP) and equate performance to DVR features in multiclass classifications.
- Published
- 2021
33. Effects of heat stress on performance and thermoregulatory responses of Piau purebred growing pigs
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Vinícius Eduardo Moreira, Renata Veroneze, Lorena Duarte Campos, Alípio dos Reis Teixeira, Paulo Henrique Reis Furtado Campos, and Sábata Cristina Januário Raimundi
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0106 biological sciences ,Male ,Respiratory rate ,Physiology ,030310 physiology ,Sus scrofa ,Thermometry ,Biology ,Loin ,010603 evolutionary biology ,01 natural sciences ,Biochemistry ,Acclimatization ,03 medical and health sciences ,Animal science ,Animals ,0303 health sciences ,Skin temperature ,Rectal temperature ,Breed ,Heat stress ,General Agricultural and Biological Sciences ,Purebred ,Heat-Shock Response ,Developmental Biology ,Body Temperature Regulation - Abstract
The study of how different breeds adapt to heat stress and the further understanding of mechanisms underlying pigs thermotolerance is of utmost importance to attenuate the negative effects of heat stress on pigs welfare, physiology, and performance. Therefore, this study aimed at evaluating the effects of ambient temperature on performance and thermoregulatory responses of Piau purebred pigs. The Brazilian native pig breed Piau is a fat-type breed characterized by adaptability and resistance to diseases. To achieve our goal, Piau purebred pigs (65 kg initial BW) were allocated to one of the two constant ambient temperature conditions: thermoneutral (22 °C; n = 11) and heat stress (30 °C; n = 11). The experimental period lasted 15 days (days 1 to 15). Pigs were individually weighed at the beginning and end of the experimental period. Body and rectal temperatures, respiratory rate and blood indicators of stress and metabolism were measured throughout the experiment. Piau purebred pigs exposed to 30 °C had lower (p 0.05) feed intake, body weight gain and final body weight than pigs at 22 °C. Feed conversion rate was not affected (p 0.05) by ambient temperature. Irrespective of ambient temperature, pigs had similar (p 0.05) backfat thickness and loin eye area. Piau pigs at 30 °C had increased (p 0.05) nape, dorsal, flank, and rectal temperatures and increased respiratory rate than their counterparts reared at 22 °C. In summary, our results show that Piau purebred pigs acclimation to 30 °C of ambient temperature is characterized by increased body skin temperature to optimize sensible heat loss to the environment; increased respiratory rate to promote latent heat loss; and by a concomitant reduced voluntary feed intake to reduce heat production associated with digestion and metabolic processes with negative effects on body weight gain.
- Published
- 2021
34. Topological Complexity and Predictability in the Dynamics of a Tumor Growth Model with Shilnikov's Chaos.
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Jorge Duarte, Cristina Januário, Carla Rodrigues, and Josep Sardanyés
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- 2013
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35. Automatic classification of idiopathic Parkinson's disease and atypical Parkinsonian syndromes combining [
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Ricardo, Martins, Francisco, Oliveira, Fradique, Moreira, Ana Paula, Moreira, Antero, Abrunhosa, Cristina, Januário, and Miguel, Castelo-Branco
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Parkinsonian Disorders ,Raclopride ,Positron-Emission Tomography ,Humans ,Parkinson Disease ,Gray Matter ,Magnetic Resonance Imaging - Published
- 2020
36. Static and Dynamic Ocular Motor Abnormalities as Potential Biomarkers in Spinocerebellar Ataxia Type 3
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João, Lemos, Ana, Novo, Cristina, Duque, Inês, Cunha, Joana, Ribeiro, João, Castelhano, and Cristina, Januário
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Adult ,Male ,Fixation, Ocular ,Machado-Joseph Disease ,Reflex, Vestibulo-Ocular ,Middle Aged ,Nystagmus, Pathologic ,Strabismus ,Ocular Motility Disorders ,Diplopia ,Saccades ,Humans ,Female ,Head Impulse Test ,Nystagmus, Optokinetic ,Biomarkers ,Aged - Abstract
While dynamic ocular motor abnormalities (e.g., gaze-evoked nystagmus (GEN), low optokinetic nystagmus (OKN), pursuit and vestibulo-ocular reflex (VOR) gains, and dysmetric saccades) have been shown to be potential biomarkers in spinocerebellar ataxia type 3 (SCA3), the value of static abnormalities (e.g., convergent [esodeviation] and divergent strabismus [exodeviation]) is unknown. Moreover, studies on dynamic abnormalities in SCA3 usually do not take into account the existence of potential abduction-adduction asymmetries in patients with degenerative ataxia. Thirty-eight patients with genetically confirmed SCA3 (24 females; mean age ± SD, 49.8± 12.2 years) and 22 healthy controls (12 females, p = 0.589; mean age ± SD, 50.7± 12.5 years, p = 0.651) underwent clinical and video-oculographic assessment. A p value 0.002 (between- and within-group analyses) and 0.001 (correlation analysis) was considered significant. Patients showed larger esodeviation at distance (p 0.001), became more esodeviated in lateral gaze (p 0.001), and their near exodeviation correlated with scale for the assessment and rating of ataxia (SARA) score (p = 0.004). Pursuit, OKN, and VOR gains were lower in patients, both for their adducting and abducting components (p 0.001). Saccades showed higher velocities (p 0.001), abducting saccades showed lower amplitude (p 0.001), and adducting saccades tended to show greater vertical bias (p = 0.018) in patients. Abducting saccades showed relatively lower velocity (p 0.001) and lower amplitude (p = 0.015) than abducting saccades within patients. All dynamic ocular motor abnormalities except saccades correlated with SARA score, CAG repeat number, and/or disease duration (p 0.001). Static and dynamic ocular motor abnormalities are potential biomarkers in SCA3. SCA3 studies using saccades should take into account the existence of potential abduction-adduction asymmetries.
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- 2020
37. Classification of Huntington’s Disease Stage with Features Derived from Structural and Diffusion-Weighted Imaging
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Rui Lavrador, Filipa Júlio, Cristina Januário, Miguel Castelo-Branco, and Gina Caetano
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Medicine (miscellaneous) ,Huntington’s disease ,grey matter density ,fractional anisotropy ,classification ,support vector machine ,basal ganglia - Abstract
The purpose of this study was to classify Huntington’s disease (HD) stage using support vector machines and measures derived from T1- and diffusion-weighted imaging. The effects of feature selection approach and combination of imaging modalities are assessed. Fourteen premanifest-HD individuals (Pre-HD; on average > 20 years from estimated disease onset), eleven early-manifest HD (Early-HD) patients, and eighteen healthy controls (HC) participated in the study. We compared three feature selection approaches: (i) whole-brain segmented grey matter (GM; voxel-based measure) or fractional anisotropy (FA) values; (ii) GM or FA values from subcortical regions-of-interest (caudate, putamen, pallidum); and (iii) automated selection of GM or FA values with the algorithm Relief-F. We assessed single- and multi-kernel approaches to classify combined GM and FA measures. Significant classifications were achieved between Early-HD and Pre-HD or HC individuals (accuracy: generally, 85% to 95%), and between Pre-HD and controls for the feature FA of the caudate ROI (74% accuracy). The combination of GM and FA measures did not result in higher performances. We demonstrate evidence on the high sensitivity of FA for the classification of the earliest Pre-HD stages, and successful distinction between HD stages.
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- 2022
38. No meio da travessia: Homenagem ao Prof. Luiz de Sousa Junior
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Jezine, Edineide, primary and Alves, Giovanna Cristina Januário, additional
- Published
- 2021
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39. Toward allele-specific targeting therapy and pharmacodynamic marker for spinocerebellar ataxia type 3
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Yari Carlomagno, Paola Giunti, Yuping Song, Bjorn Oskarsson, Jan O. Aasly, Rana Hanna Al-Shaikh, Robin Labrum, Zbigniew K. Wszolek, James M. Polke, João Lemos, Henry L. Paulson, Guojun Bu, Eric R. Eggenberger, Karen Jansen-West, William D. Freeman, Hector Garcia-Moreno, Mercedes Prudencio, Marka van Blitterswijk, Osamu Onodera, Joseph H. Friedman, Ryan J. Uitti, Inês Gomes, Hayley S. McLoughlin, Mark S. LeDoux, Takuya Konno, Venka Veerappan, Nathan P. Staff, Leonard Petrucelli, John N. Caviness, Cristina Januário, Tania F. Gendron, Lillian M. Daughrity, Mari Tada, Iris Vanessa Marin Collazo, Andreas Puschmann, Takeshi Ikeuchi, Katharine Nicholson, Josephine F. Huang, Klaas J. Wierenga, Sorina Gorcenco, Christin Karremo, Matthew R. Spiegel, Akiyoshi Kakita, Jay A. van Gerpen, Judith A. Dunmore, Ronald F. Pfeiffer, Philip W. Tipton, John D. Fryer, Mark R. Pittelkow, Vikram G. Shakkottai, Natalie Byron, and Michael G. Heckman
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Neurons ,congenital, hereditary, and neonatal diseases and abnormalities ,medicine.medical_specialty ,Neurology ,business.industry ,Mutant ,Machado-Joseph Disease ,General Medicine ,medicine.disease ,Bioinformatics ,Article ,Repressor Proteins ,Clinical trial ,Polymorphism (computer science) ,Spinocerebellar ataxia ,medicine ,Humans ,Allele ,Ataxin-3 ,business ,Trinucleotide repeat expansion ,Gene ,Alleles - Abstract
Spinocerebellar ataxia type 3 (SCA3), caused by a CAG repeat expansion in the ataxin-3 gene (ATXN3), is characterized by neuronal polyglutamine (polyQ) ATXN3 protein aggregates. Although there is no cure for SCA3, gene-silencing approaches to reduce toxic polyQ ATXN3 showed promise in preclinical models. However, a major limitation in translating putative treatments for this rare disease to the clinic is the lack of pharmacodynamic markers for use in clinical trials. Here, we developed an immunoassay that readily detects polyQ ATXN3 proteins in human biological fluids and discriminates patients with SCA3 from healthy controls and individuals with other ataxias. We show that polyQ ATXN3 serves as a marker of target engagement in human fibroblasts, which may bode well for its use in clinical trials. Last, we identified a single-nucleotide polymorphism that strongly associates with the expanded allele, thus providing an exciting drug target to abrogate detrimental events initiated by mutant ATXN3. Gene-silencing strategies for several repeat diseases are well under way, and our results are expected to improve clinical trial preparedness for SCA3 therapies.
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- 2020
40. Cutaneous adverse drug reaction after continuous subcutaneous apomorphine infusion
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Cristina Januário, Joana Calvão, Fradique Moreira, José Carlos Cardoso, and Margarida Gonçalo
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Parkinson's disease ,Apomorphine ,Drug-Related Side Effects and Adverse Reactions ,business.industry ,Injections, Subcutaneous ,Parkinson Disease ,Dermatology ,Sodium metabisulfite ,Pharmacology ,medicine.disease ,chemistry.chemical_compound ,Infectious Diseases ,chemistry ,Maculopapular exanthema ,Medicine ,Humans ,business ,Adverse drug reaction ,medicine.drug ,Skin - Published
- 2020
41. Eventos adversos relacionados a administração de medicamentos em um hospital do Vale do Taquari
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Alves, Kellen Cristina Januário Benedetti, Lohmann, Paula Michele, da Costa, Arlete Eli Kunz, and dos Santos, Fernanda
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lcsh:Internal medicine ,Erros de medicação. Segurança do paciente. Sistemas de notificação de reações adversas a medicamentos ,Medication errors. Patient safety. Adverse Drug Reaction Notification Systems ,lcsh:R ,lcsh:Medicine ,erros de medicação. segurança do paciente. sistemas de notificação de reações adversas a medicamentos ,lcsh:RC109-216 ,lcsh:RC31-1245 ,Errores de medicación. Seguridad del paciente. Sistemas de notificación de reacciones adversas a medicamentos ,lcsh:Infectious and parasitic diseases - Abstract
Background and Objectives: The medication process in the hospital environment is one of the most important activities to ensure efficacy in the patient's therapy. The objective of this study was to know the perception of the nursing team about the occurrence of adverse events related to drug administration in a hospital in Vale do Taquari, Rio Grande do Sul. Methods: This is a descriptive exploratory study with a qualitative approach-quantitative. Data were collected through the application of a questionnaire to nursing professionals, constituting a sample of fifty-eight interviewees. The analysis was made through descriptive statistics. Results: Of the participants, 96.6% knew the steps to avoid medication errors, and 79.3% had already made some mistake in their professional practices in the institution. The most frequent error was the erroneous administration dose of 43.5%, with 97.3% of the professionals already perceived some error in the medication process and 30% considered the work overload to be an aggravating factor for the occurrence of errors. Regarding the professional's behavior regarding the error, 80.4% communicated verbally to the supervising professional and as a consequence received verbal guidance 78.3% of the time. As for feelings about the error 36.8% revealed to feel fear of harming the patient. Conclusion: These findings point to serious potential errors that impair patient safety; however, it is noted that verbal guidelines are offered avoiding punishment, but the incentive for reporting the error is precarious, making it difficult for the team to identify potential errors and implement barriers to avoid them. Justificación y Objetivos: El proceso de medicación en el ambiente hospitalario es una de las actividades de mayor importancia para asegurar la eficacia en la terapéutica del paciente. El estudio tuvo como objetivo conocer la percepción del equipo de enfermería sobre la ocurrencia de eventos adversos relacionados con la administración de medicamentos en un hospital del Valle del Taquari, Rio Grande do Sul. Métodos: Se trata de un estudio exploratorio descriptivo, con abordaje cuali-quantitativo. Los datos fueron recolectados por medio de la aplicación de un cuestionario a los profesionales de la enfermería, constituyendo una muestra de cincuenta y ocho entrevistados. El análisis fue realizado por medio de estadística descriptiva. Resultados: De los participantes 96,6% conocían las etapas para evitar errores de medicación y el 79,3% ya cometieron algún error en sus prácticas profesionales en la institución. El error más frecuente fue la dosis de administración equivocada 43,5%, siendo que el 97,3% de los profesionales ya percibieron algún error en el proceso de medicación y el 30% considera la sobrecarga de trabajo un factor agravante para la ocurrencia de errores. En cuanto a la conducta del profesional frente al error 80,4% comunicaban verbalmente al profesional supervisor y como consecuencia recibieron orientación verbal el 78,3% de las veces. En cuanto a los sentimientos frente al error 36,8% revelaron sentir miedo de perjudicar al paciente. Conclusión: Tales hallazgos apuntan errores potenciales graves que perjudican la seguridad del paciente, pero se nota que las orientaciones verbales son ofrecidas evitando el castigo, pero el incentivo para el registro de notificación del error es precario, dificultando el equipo de identificar los errores potenciales e implementar barreras para evitarlos Justificativa e Objetivos: O processo de medicação no ambiente hospitalar é uma das atividades de maior importância para assegurar a eficácia na terapêutica do paciente. Este estudo teve como objetivo conhecer a percepção da equipe de enfermagem sobre a ocorrência de eventos adversos relacionados a administração de medicamentos em um hospital do Vale do Taquari, Rio Grande do Sul. Métodos: Trata-se de um estudo exploratório descritivo, com abordagem quali-quantitativa. Os dados foram coletados por meio da aplicação de um questionário aos profissionais da enfermagem, constituindo uma amostra de cinquenta e oito entrevistados. A análise foi feita por meio de estatística descritiva. Resultados: Dos participantes, 96,6% conheciam as etapas para evitar erros de medicação, e 79,3% já cometeram algum erro em suas práticas profissionais na instituição. O erro mais frequente foi a dose de administração errada, 43,5%, sendo que 97,3% dos profissionais já perceberam algum erro no processo de medicação e 30% consideram a sobrecarga de trabalho um fator agravante para a ocorrência de erros. Quanto à conduta do profissional frente ao erro, 80,4% comunicavam verbalmente ao profissional supervisor e como consequência receberam orientação verbal, 78,3% das vezes. Quanto aos sentimentos frente ao erro 36,8% revelaram sentir medo de prejudicar o paciente. Conclusão: Tais achados apontam erros potenciais graves que prejudicam a segurança do paciente, no entanto, nota-se que orientações verbais são oferecidas evitando a punição, mas o incentivo para o registro de notificação do erro é precário, dificultando a equipe de identificar os erros potenciais e implementar barreiras para evitá-los.
- Published
- 2020
42. Progression of motor symptoms in Parkinson's disease
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Daniela Vieira, Joana Jesus-Ribeiro, and Cristina Januário
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Levodopa ,medicine.medical_specialty ,Parkinson's disease ,Gait Disturbance ,business.industry ,Postural instability ,Disease ,medicine.disease ,Motor symptoms ,Physical medicine and rehabilitation ,Neuroimaging ,medicine ,Cognitive decline ,business ,medicine.drug - Abstract
Motor impairment in Parkinson's disease (PD) is characterized by different signs that include bradykinesia, rigidity, tremor, and postural instability. Manifestations are heterogeneous among patients and progress throughout the course of the disease. There is evidence that tremor-dominant patients have a slower progression of motor impairment than akinetic-rigid ones. Some studies have identified predictors of disease progression. The presence of cognitive decline at baseline is associated with faster motor impairment, whereas late-onset disease, the presence of, gait disturbance and postural instability at baseline are associated with more disability. There is an increasing interest in defining a biomarker for disease progression. Some genetic, biochemical, and neuroimaging studies found potential biomarkers, but none has fulfilled all the required characteristics. Treatment of PD patients becomes more difficult at later stages of the disease, with a progressive loss of levodopa effectiveness and the development of motor fluctuations. Future investigations are needed to develop a disease-modifying drug that ensures better disease management.
- Published
- 2020
43. Contributors
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Hesham Abboud, Noha F. Abdelkader, Miguel A. Abellanas, Warwick R. Adams, Mikko Airavaara, Ibrahim S. Al-Busaidi, Yassar Alamri, Stephanie L. Alberico, Athanasios Alexiou, Lorena Rosa S. Almeida, Ryan S. Anderton, Veronica Antipova, Emanuela Aragona, Alessandro Arrigo, Ghulam Md Ashraf, Manon Auffret, Maria S. Aymerich, Megan Bakeberg, Graham R. Barnes, Lazaros Belbasis, Vanesa Bellou, Fabrizio Benedetti, Patrick Benson, Leandro Bueno Bergantin, Roongroj Bhidayasiri, Mariza Bortolanza, Michelle Byrnes, Alessandro Calamuneri, Afonso Caricati-Neto, Elisa Carlino, Sapana Sameer Chaudhary, Sameer Chaudhary, Piotr Chmielarz, Ana Paula Chuproski, Elaine Del-Bel, Marco De Risi, J.C. Devedjian, D. Devos, Giancarlo Di Gennaro, Andrii Domanskyi, Patrícia Dominico, Sophie Drapier, J.A. Duce, Aloke K. Dutta, David Eidelberg, Evangelos Evangelou, Juliane Fagotti, Lucia Farotti, M. Fisichella, Maria João Forjaz, Elisa Frisaldi, Anselm B.M. Fuermaier, Kikuro Fukushima, Junko Fukushima, Michele Gaeta, Luke Gordon, Alexander Hawlitschka, Carsten Holzmann, Josephien Jansen, Cristina Januário, Joana Jesus-Ribeiro, Daniel M. Johnstone, Janneke Koerts, Julia Konovalova, Jens Kurth, C. Lachaud, C. Laloux, Michele Lanotte, Peter A. LeWitt, Marcelo M.S. Lima, Tobias Lindner, K.F. Loewenbrück, Leonardo Lopiano, Dan Luo, Teresa Mann, Peizhong Mao, Yifan Mao, Pablo Martinez–Martin, Frank L. Mastaglia, Yasuaki Mizutani, Nicola Modugno, C. Moreau, Enricomaria Mormina, Thomas Müller, Tatsuro Mutoh, Kanae Nagao, Nandakumar S. Narayanan, Glauce Crivelaro Nascimento, Saieswari Natesan, Lee Neilson, Yoshiki Niimi, Enrica Olivola, F. Pan-Montojo, Anne Panhelainen, Federico Paolini Paoletti, Tanvee Pardeshi, Lucilla Parnetti, Serene S. Paul, Matej Perovnik, Claudia Petrucco, Alessandro Piedimonte, Sakshi Rawat, H. Reichmann, Maarten Reith, Maddeson Riley, Carmen Rodríguez-Blázquez, Petra Bago Rožanković, Tomaž Rus, Ryuji Sakakibara, Bruno L. Santos-Lobato, Fúlvio Alexandre Scorza, Jonathan Stone, Chris C. Tang, Adriano D.S. Targa, Fuyuki Tateno, Petra Tomše, Maja Trošt, John T.H. Tsiang, Lara Tucha, Oliver Tucha, Tomoyuki Uchiyama, Guilherme T. Valenca, Marc Vérin, Daniela Vieira, Gregory L. Willis, Martin Witt, Benjamin K.P. Woo, Andreas Wree, Tatsuya Yamamoto, Jonathan Zande, and Maurizio Zibetti
- Published
- 2020
44. Chaos in Ecology: The Topological Entropy of a Tritrophic Food Chain Model
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Jorge Duarte, Cristina Januário, and Nuno Martins
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Mathematics ,QA1-939 - Abstract
An ecosystem is a web of complex interactions among species. With the purpose of understanding this complexity, it is necessary to study basic food chain dynamics with preys, predators and superpredators interactions. Although there is an elegant interpretation of ecological models in terms of chaos theory, the complex behavior of chaotic food chain systems is not completely understood. In the present work we study a specific food chain model from the literature. Using results from symbolic dynamics, we characterize the topological entropy of a family of logistic-like Poincaré return maps that replicates salient aspects of the dynamics of the model. The analysis of the variation of this numerical invariant, in some realistic system parameter region, allows us to quantify and to distinguish different chaotic regimes. This work is still another illustration of the role that the theory of dynamical systems can play in the study of chaotic dynamics in life sciences.
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- 2008
- Full Text
- View/download PDF
45. Visual and ocular motor function in the atypical form of neurodegeneration with brain iron accumulation type I
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João Lemos, A. Matos, Miguel Castelo-Branco, Cláudia Farinha, Joana Jesus-Ribeiro, Margarida Amorim, Aldina Reis, and Cristina Januário
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Male ,Pathology ,Visual acuity ,Eye Movements ,genetic structures ,Neurodegeneration with brain iron accumulation ,Visual Acuity ,Ocular Motility Disorders ,0302 clinical medicine ,Fluorescein Angiography ,Doenças da Retina ,Distúrbios do Metabolismo do Ferro ,medicine.diagnostic_test ,Acuidade Visual ,Middle Aged ,Sensory Systems ,Optic nerve ,Female ,medicine.symptom ,Erg ,Tomography, Optical Coherence ,Retinopathy ,Photopic vision ,Adult ,Distrofias Neuroaxonais ,medicine.medical_specialty ,Fundus Oculi ,Neuroaxonal Dystrophies ,Retina ,Young Adult ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,Retinal Diseases ,Electroretinography ,medicine ,Humans ,business.industry ,medicine.disease ,Iron Metabolism Disorders ,eye diseases ,Ophthalmology ,Cross-Sectional Studies ,030221 ophthalmology & optometry ,Evoked Potentials, Visual ,Potenciais Evocados Visuais ,sense organs ,Perturbações da Motilidade Ocular ,business ,030217 neurology & neurosurgery - Abstract
Background/aimsNeurodegeneration with brain iron accumulation (NBIA) type I is a rare disease that can be divided into a classical or atypical variant, according to age of onset and clinical pattern. Neuro-ophthalmological involvement has been documented in the classical variant but only anecdotically in the atypical variant. We sought to describe the visual and ocular motor function in patients with atypical form of NBIA type I.MethodsCross-sectional study, including patients with genetically confirmed NBIA type I and classified as atypical variant, who underwent ophthalmological examination with best corrected visual acuity (BCVA), optical coherence tomography (OCT), fundus autofluorescence (FAF), electroretinography (ERG), visual evoked potentials (VEP) and video-oculography.ResultsSeven patients with a mean BCVA of 0.12±0.14 logMAR were included. Only two patients showed structural evidence of advanced retinopathy in OCT and FAF, and there were no cases of optic atrophy. ERG data, however, showed abnormal scotopic and/or photopic responses in all patients. VEP were normal in all three patients. Ocular fixation was markedly unstable (eg, increased rate of saccadic pulses) in the majority of patients (5). Additional mild ocular motor disturbances included low gain pursuit (2), hypermetric saccades (1), low gain optokinetic (2) and caloric and rotatory responses (3).ConclusionFunctional retinal changes associated with marked instability of ocular fixation should be included in the clinical spectrum of NBIA, particularly in the atypical form.
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- 2017
46. Optimal homotopy analysis of a chaotic HIV-1 model incorporating AIDS-related cancer cells
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C. Correia Ramos, Jorge Duarte, Nuno Martins, Cristina Januário, Josep Sardanyés, Carla Rodrigues, and Brezinski, Claude
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0301 basic medicine ,education.field_of_study ,Applied Mathematics ,Homotopy ,Cancer Model ,Population ,Chaotic ,Lyapunov exponent ,01 natural sciences ,Quantitative Biology::Cell Behavior ,010305 fluids & plasmas ,03 medical and health sciences ,symbols.namesake ,030104 developmental biology ,0103 physical sciences ,Attractor ,symbols ,Applied mathematics ,Observability ,education ,Homotopy analysis method ,Mathematics - Abstract
The studies of nonlinear models in epidemiology have generated a deep interest in gaining insight into the mechanisms that underlie AIDS-related cancers, providing us with a better understanding of cancer immunity and viral oncogenesis. In this article, we analyze an HIV-1 model incorporating the relations between three dynamical variables: cancer cells, healthy CD4 + T lymphocytes, and infected CD4 + T lymphocytes. Recent theoretical investigations indicate that these cells interactions lead to different dynamical outcomes, for instance to periodic or chaotic behavior. Firstly, we analytically prove the boundedness of the trajectories in the system’s attractor. The complexity of the coupling between the dynamical variables is quantified using observability indices. Our calculations reveal that the highest observable variable is the population of cancer cells, thus indicating that these cells could be monitored in future experiments in order to obtain time series for attractor’s reconstruction. We identify different dynamical behaviors of the system varying two biologically meaningful parameters: r 1, representing the uncontrolled proliferation rate of cancer cells, and k 1, denoting the immune system’s killing rate of cancer cells. The maximum Lyapunov exponent is computed to identify the chaotic regimes. Considering very recent developments in the literature related to the homotopy analysis method (HAM), we calculate the explicit series solutions of the cancer model and focus our analysis on the dynamical variable with the highest observability index. An optimal homotopy analysis approach is used to improve the computational efficiency of HAM by means of appropriate values for the convergence control parameter, which greatly accelerate the convergence of the series solution. The approximated analytical solutions are used to compute density plots, which allow us to discuss additional dynamical features of the model.
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- 2017
47. Cortical control of vertical and horizontal saccades in progressive supranuclear palsy: An exploratory fMRI study
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Cristina Januário, Miguel Castelo-Branco, João Lemos, Gil Cunha, João Castelhano, António Freire, Diliana Rebelo, Luciano Almendra, Miguel Patrício, Dina Pereira, and Luís Cunha
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Male ,Neuropsychological Tests ,050105 experimental psychology ,Progressive supranuclear palsy ,03 medical and health sciences ,0302 clinical medicine ,Neural Pathways ,Saccades ,medicine ,Humans ,0501 psychology and cognitive sciences ,Eye Movement Measurements ,Default mode network ,Aged ,Aged, 80 and over ,Cerebral Cortex ,Brain Mapping ,05 social sciences ,Vertical plane ,Middle Aged ,medicine.disease ,Magnetic Resonance Imaging ,eye diseases ,Saccadic masking ,Oxygen ,Neurology ,Cerebrovascular Circulation ,Saccade ,Eye tracking ,Female ,Supranuclear Palsy, Progressive ,Neurology (clinical) ,Brainstem ,Psychology ,Hypoactivity ,Neuroscience ,030217 neurology & neurosurgery - Abstract
Progressive supranuclear palsy (PSP) is a neurodegenerative disorder showing predominant brainstem involvement, characterized by marked slowing of rapid eye movements (saccades), particularly along the vertical plane. While the contribution of the brainstem damage for the saccadic disturbance in PSP has been extensively studied, much less is known about its cortical and subcortical pathomechanisms. We measured reflexive (prosaccades) and voluntary (antisaccades) saccades in the vertical and horizontal plane in PSP patients (n=8) and controls (n=10) in an eye tracking study, followed by the measurement of blood oxygenation-level dependent (BOLD) activation (PSP, n=6; controls, n=10) during similar saccade paradigms. Behaviorally, PSP patients evidenced slower and lower amplitude prosaccades (horizontal and vertical) and lower amplitude antisaccades (vertical) than controls. Functionally, patients showed decreased frontostriatal BOLD activation during prosaccades (horizontal and vertical) and antisaccades (vertical), relative to controls. Additionally, PSP patients showed less default mode network (DMN) deactivation than controls for all types of saccades. Within groups, controls showed no BOLD differences between horizontal and vertical prosaccades while PSP patients demonstrated greater DMN deactivation during vertical prosaccades. Both groups evidenced greater DMN deactivation during vertical antisaccades when compared to their horizontal counterpart and patients further showed relative frontostriatal BOLD hypoactivity during vertical antisaccades. We found fMRI evidence of frontostriatal hypoactivity in PSP patients relative to controls, especially during vertical saccades. These new findings highlight the impact of cortical impairment in saccadic disturbance of PSP.
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- 2017
48. A chaotic bursting-spiking transition in a pancreatic beta-cells system: observation of an interior glucose-induced crisis
- Author
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Jorge Duarte, Nuno Martins, and Cristina Januário
- Subjects
Dynamical systems theory ,Entropy ,Biophysics ,Symbolic dynamics ,Chaotic ,Topological entropy ,Lyapunov exponent ,Analytical solutions ,Parameter space ,Models, Biological ,01 natural sciences ,010305 fluids & plasmas ,Glucose-induced interior chaotic crisis ,Bursting ,symbols.namesake ,Control theory ,Insulin-Secreting Cells ,0103 physical sciences ,Attractor ,Humans ,Statistical physics ,010301 acoustics ,Physics ,Pancreatic beta-cells system ,Applied Mathematics ,General Medicine ,Electrophysiological Phenomena ,Return maps ,Computational Mathematics ,Glucose ,Modeling and Simulation ,symbols ,General Agricultural and Biological Sciences - Abstract
Nonlinear systems are commonly able to display abrupt qualitative changes (or transitions) in the dynamics. A particular type of these transitions occurs when the size of a chaotic attractor suddenly changes. In this article, we present such a transition through the observation of a chaotic interior crisis in the Deng bursting-spiking model for the glucose-induced electrical activity of pancreatic $β $-cells. To this chaos-chaos transition corresponds precisely the change between the bursting and spiking dynamics, which are central and key dynamical regimes that the Deng model is able to perform. We provide a description of the crisis mechanism at the bursting-spiking transition point in terms of time series variations and based on certain amplitudes of invariant intervals associated with return maps. Using symbolic dynamics, we are able to accurately compute the points of a curve representing the transition between the bursting and spiking regimes in a biophysical meaningfully parameter space. The analysis of the chaotic interior crisis is complemented by means of topological invariants with the computation of the topological entropy and the maximum Lyapunov exponent. Considering very recent developments in the literature, we construct analytical solutions triggering the bursting-spiking transition in the Deng model. This study provides an illustration of how an integrated approach, involving numerical evidences and theoretical reasoning within the theory of dynamical systems, can directly enhance our understanding of biophysically motivated models.
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- 2017
49. Eventos adversos relacionados a administração de medicamentos em um hospital do Vale do Taquari
- Author
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Paula Michele Lohmann, Kellen Cristina Januário Benedetti Alves, Fernanda dos Santos, and Arlete Eli Kunz da Costa
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Descriptive statistics ,business.industry ,media_common.quotation_subject ,Exploratory research ,Mistake ,Sample (statistics) ,General Medicine ,Patient safety ,Incentive ,Nursing ,Feeling ,Medicine ,business ,Adverse effect ,media_common - Abstract
Justificativa e Objetivos: O processo de medicação no ambiente hospitalar é uma das atividades de maior importância para assegurar a eficácia na terapêutica do paciente. Este estudo teve como objetivo conhecer a percepção da equipe de enfermagem sobre a ocorrência de eventos adversos relacionados a administração de medicamentos em um hospital do Vale do Taquari, Rio Grande do Sul. Métodos: Trata-se de um estudo exploratório descritivo, com abordagem quali-quantitativa. Os dados foram coletados por meio da aplicação de um questionário aos profissionais da enfermagem, constituindo uma amostra de cinquenta e oito entrevistados. A análise foi feita por meio de estatística descritiva. Resultados: Dos participantes, 96,6% conheciam as etapas para evitar erros de medicação, e 79,3% já cometeram algum erro em suas práticas profissionais na instituição. O erro mais frequente foi a dose de administração errada, 43,5%, sendo que 97,3% dos profissionais já perceberam algum erro no processo de medicação e 30% consideram a sobrecarga de trabalho um fator agravante para a ocorrência de erros. Quanto à conduta do profissional frente ao erro, 80,4% comunicavam verbalmente ao profissional supervisor e como consequência receberam orientação verbal, 78,3% das vezes. Quanto aos sentimentos frente ao erro 36,8% revelaram sentir medo de prejudicar o paciente. Conclusão: Tais achados apontam erros potenciais graves que prejudicam a segurança do paciente, no entanto, nota-se que orientações verbais são oferecidas evitando a punição, mas o incentivo para o registro de notificação do erro é precário, dificultando a equipe de identificar os erros potenciais e implementar barreiras para evitá-los.
- Published
- 2019
50. Dopamine dysregulation syndrome induced by proxy
- Author
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Diogo Reis Carneiro, Ana Morgadinho, Cristina Januário, and Mário Sousa
- Subjects
0301 basic medicine ,Pediatrics ,medicine.medical_specialty ,Parkinson's disease ,Substance-Related Disorders ,Unusual Association of Diseases/Symptoms ,Disease ,030105 genetics & heredity ,Levodopa ,03 medical and health sciences ,Social support ,0302 clinical medicine ,Dopamine ,medicine ,Humans ,Dopamine dysregulation syndrome ,Aged ,business.industry ,Dopaminergic ,Carbidopa ,Parkinson Disease ,General Medicine ,Syndrome ,medicine.disease ,Disruptive, Impulse Control, and Conduct Disorders ,Drug Combinations ,Dyskinesia ,Female ,medicine.symptom ,Complication ,business ,030217 neurology & neurosurgery ,medicine.drug - Abstract
Dopamine dysregulation syndrome is a rare complication of Parkinson’s disease (PD) treatment. We present a 70-year-old woman with a long-standing PD and a clinical picture compatible with dopaminergic dysregulation, which was ultimately revealed to be induced by her companion. Patient’s exuberant choreiform dyskinesia led to a potential financial advantage when performed outside the hospital but excessive dopamine intake also occurred during hospital admission, without any obvious reward for the abuser. Even in cases where there is no place for a definitive diagnosis, deceptive behaviours must be identified as their management is based on psychological and social support in parallel to the adjustment of PD therapy.
- Published
- 2019
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