20 results on '"Crippa GE"'
Search Results
2. Study comparing midazolam and nitrous oxide in dental anxiety control.
- Author
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Pereira-Santos D, Brêda-Júnior MA, Ferraz EP, Crippa GE, de Oliveira FS, and da Rocha-Barros VM
- Subjects
- Adolescent, Adult, Dental Anxiety psychology, Follow-Up Studies, Humans, Hydrocortisone analysis, Male, Mandible surgery, Molar, Third surgery, Oxygen administration & dosage, Saliva chemistry, Tooth Extraction methods, Young Adult, Anesthesia, Dental methods, Anesthetics, Inhalation administration & dosage, Conscious Sedation methods, Dental Anxiety prevention & control, Hypnotics and Sedatives administration & dosage, Midazolam administration & dosage, Nitrous Oxide administration & dosage
- Abstract
The purpose of this study is to comparatively assess the effect of midazolam and nitrous oxide associated with oxygen, in lower third molar extractions, on the change in the anxiety level of patients by salivary cortisol dosage. Twenty-eight male patients underwent lower third molar extraction under sedation with midazolam and nitrous oxide. Objective (salivary cortisol dosage) and subjective (Corah Dental Anxiety Scale) data have been obtained. By salivary cortisol, 40 minutes after midazolam administration, there has been a statistically significant difference compared with the mean baseline value. Midazolam was the most effective sedation method for reducing salivary cortisol level.
- Published
- 2013
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3. Bone tissue, cellular, and molecular responses to titanium implants treated by anodic spark deposition.
- Author
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Sverzut AT, de Albuquerque GC, Crippa GE, Chiesa R, Della Valle C, de Oliveira PT, Beloti MM, and Rosa AL
- Subjects
- Animals, Cell Proliferation, Cells, Cultured, Coated Materials, Biocompatible metabolism, Dogs, Electrodes, Mandible ultrastructure, Osteoblasts cytology, Surface Properties, Titanium metabolism, Coated Materials, Biocompatible chemistry, Dental Prosthesis Design, Mandible surgery, Osseointegration, Titanium chemistry
- Abstract
A myriad of titanium (Ti) surface modifications has been proposed to hasten the osseointegration. In this context, the aim of this study was to perform histomorphometric, cellular, and molecular analyses of the bone tissue grown in close contact with Ti implants treated by anodic spark deposition (ASD-AK). Acid-etched (AE) Ti implants either untreated or submitted to ASD-AK were placed into dog mandibles and retrieved at 3 and 8 weeks. It was noticed that both implants, AE and ASD-AK, were osseointegrated at 3 and 8 weeks. Histomorphometric analysis showed differences between treatments only for bone-to-implant contact, being higher on AE implants. Although not backed by histomorphometric results, gene expression of key bone markers was higher for bone grown in close contact with ASD-AK and for cells harvested from these fragments and cultured until subconfluence. Cell proliferation at days 7 and 10 and alkaline phosphatase activity at day 10 was higher on AE surfaces. No statistical significant difference was noticed for extracellular matrix mineralization at 17 days. Our results have shown that the Ti fixtures treated by ASD-AK allowed in vivo osseointegration and induced higher expression of key markers of osteoblast phenotype, suggesting that this surface treatment could be considered to produce implants for clinical applications., (Copyright © 2012 Wiley Periodicals, Inc.)
- Published
- 2012
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4. The influence of pore size on osteoblast phenotype expression in cultures grown on porous titanium.
- Author
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Teixeira LN, Crippa GE, Lefebvre LP, De Oliveira PT, Rosa AL, and Beloti MM
- Subjects
- Adult, Alveolar Process cytology, Cell Culture Techniques, Cell Differentiation, Cells, Cultured, Humans, Male, Porosity, Surface Properties, Young Adult, Cell Proliferation, Osteoblasts cytology, Titanium chemistry
- Abstract
This study investigated the effect of pore size on osteoblastic phenotype development in cultures grown on porous titanium (Ti). Porous Ti discs with three different pore sizes, 312 μm (Ti 312), 130 μm (Ti 130) and 62 μm (Ti 62) were fabricated using a powder metallurgy process. Osteoblastic cells obtained from human alveolar bone were cultured on porous Ti samples for periods of up to 14 days. Cell proliferation was affected by pore size at day 3 (p=0.0010), day 7 (p=0.0005) and day 10 (p=0.0090) in the following way: Ti 62
- Published
- 2012
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5. Effect of low-level laser therapy after rapid maxillary expansion on proliferation and differentiation of osteoblastic cells.
- Author
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da Silva AP, Petri AD, Crippa GE, Stuani AS, Stuani AS, Rosa AL, and Stuani MB
- Subjects
- Animals, Calcification, Physiologic, Cell Culture Techniques, Gene Expression, Male, Osteoblasts metabolism, Osteoblasts radiation effects, Osteogenesis radiation effects, Palatal Expansion Technique, Rats, Rats, Wistar, Real-Time Polymerase Chain Reaction, Alkaline Phosphatase metabolism, Cell Differentiation radiation effects, Cell Proliferation radiation effects, Low-Level Light Therapy methods, Osteoblasts cytology
- Abstract
The aim of this study was to investigate the osteoblastic activity of cells derived from the midpalatal suture upon treatment with low-level laser therapy (LLLT) after rapid maxillary expansion (RME). A total of 30 rats were divided into two groups: experimental I (15 rats with RME without LLLT) and experimental II (15 rats with RME + LLLT). The rats were euthanized at 24 h, 48 h, and 7 days after RME, when the osteoblastic cells derived from the rats' midpalatal suture were explanted. These cells were cultured for periods up to 17 days, and then in vitro osteogenesis parameters and gene expression markers were evaluated. The cellular doubling time in the proliferative stage (3-7 days) was decreased in cultured cells harvested from the midpalatal suture at 24 and 48 h after RME + LLLT, as indicated by the increased growth of the cells in a culture. Alkaline phosphatase activity at days 7 and 14 of the culture was increased by LLLT in cells explanted from the midpalatal suture at 24 and 48 h and 7 days after RME. The mineralization at day 17 was increased by LLLT after RME in all periods. Results from the real-time PCR demonstrated that cells harvested from the LLLT after RME group showed higher levels of ALP, Runx2, osteocalcin, type I collagen, and bone sialoprotein mRNA than control cells. More pronounced effects on ALP activity, mineralization, and gene expression of bone markers were observed at 48 h after RME and LLLT. These results indicate that the LLLT applied after RME is able to increase the proliferation and the expression of an osteoblastic phenotype in cells derived from the midpalatal suture.
- Published
- 2012
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6. Effects of type I collagen coating on titanium osseointegration: histomorphometric, cellular and molecular analyses.
- Author
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Sverzut AT, Crippa GE, Morra M, de Oliveira PT, Beloti MM, and Rosa AL
- Subjects
- Animals, Dental Materials chemical synthesis, Dogs, Mandible physiology, Coated Materials, Biocompatible chemical synthesis, Collagen Type I chemistry, Dental Implants, Mandible pathology, Mandible surgery, Osseointegration physiology, Titanium chemistry
- Abstract
The investigation of titanium (Ti) surface modifications aiming to increase implant osseointegration is one of the most active research areas in dental implantology. This study was carried out to evaluate the benefits of coating Ti with type I collagen on the osseointegration of dental implants. Acid etched Ti implants (AETi), either untreated or coated with type I collagen (ColTi), were placed in dog mandibles for three and eight weeks for histomorphometric, cellular and molecular evaluations of bone tissue response. While the histological aspects were essentially the same with both implants being surrounded by lamellar bone trabeculae, histomorphometric analysis showed more abundant bone formation in ColTi, mainly at three weeks. Cellular evaluation showed that cells harvested from bone fragments in close contact with ColTi display lower proliferative capacity and higher alkaline phosphatase activity, phenotypic features associated with more differentiated osteoblasts. Confirming these findings, molecular analyses showed that ColTi implants up-regulates the expression of a panel of genes well known as osteoblast markers. Our results present a set of evidences that coating AETi with collagen fastens the osseointegration by stimulating bone formation at the cellular and molecular levels, making this combination of morphological and biochemical modification a promising approach to treat Ti surfaces.
- Published
- 2012
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7. Pore size regulates cell and tissue interactions with PLGA-CaP scaffolds used for bone engineering.
- Author
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Sicchieri LG, Crippa GE, de Oliveira PT, Beloti MM, and Rosa AL
- Subjects
- Alkaline Phosphatase metabolism, Animals, Biomarkers metabolism, Bone and Bones physiology, Cell Proliferation drug effects, Cells, Cultured, Gene Expression Regulation drug effects, Male, Microscopy, Electron, Scanning, Osteoblasts enzymology, Osteogenesis drug effects, Polylactic Acid-Polyglycolic Acid Copolymer, Porosity drug effects, Rats, Rats, Wistar, Skull blood supply, Skull drug effects, Skull pathology, Bone and Bones drug effects, Calcium Phosphates pharmacology, Cell Communication drug effects, Lactic Acid pharmacology, Osteoblasts cytology, Polyglycolic Acid pharmacology, Tissue Engineering methods, Tissue Scaffolds chemistry
- Abstract
A common subject in bone tissue engineering is the need for porous scaffolds to support cell and tissue interactions aiming at repairing bone tissue. As poly(lactide-co-glycolide)-calcium phosphate (PLGA-CaP) scaffolds can be manufactured with different pore sizes, the aim of this study was to evaluate the effect of pore diameter on osteoblastic cell responses and bone tissue formation. Scaffolds were prepared with 85% porosity, with pore diameters in the ranges 470-590, 590-850 and 850-1200 µm. Rat bone marrow stem cells differentiated into osteoblasts were cultured on the scaffolds for up to 10 days to evaluate cell growth, alkaline phosphatase (ALP) activity and the gene expression of the osteoblast markers RUNX2, OSX, COL, MSX2, ALP, OC and BSP by real-time PCR. Scaffolds were implanted in critical size rat calvarial defects for 2, 4, and 8 weeks for histomorphometric analysis. Cell growth and ALP activity were not affected by the pore size; however, there was an increase in the gene expression of osteoblastic markers with the increase in the pore sizes. At 2 weeks all scaffolds displayed a similar amount of bone and blood vessels formation. At 4 and 8 weeks much more bone formation and an increased number of blood vessels were observed in scaffolds with pores of 470-590 µm. These results show that PLGA-CaP is a promising biomaterial for bone engineering. However, ideally, combinations of larger (-1000 µm) and smaller (-500 µm) pores in a single scaffold would optimize cellular and tissue responses during bone healing., (Copyright © 2011 John Wiley & Sons, Ltd.)
- Published
- 2012
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8. Response of human alveolar bone-derived cells to a novel poly(vinylidene fluoride-trifluoroethylene)/barium titanate membrane.
- Author
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Teixeira LN, Crippa GE, Gimenes R, Zaghete MA, de Oliveira PT, Rosa AL, and Beloti MM
- Subjects
- Apoptosis drug effects, Apoptosis genetics, Barium Compounds pharmacology, Bone Regeneration drug effects, Bone Regeneration genetics, Bone Regeneration physiology, Bone and Bones cytology, Bone and Bones metabolism, Cells, Cultured, Core Binding Factor Alpha 1 Subunit genetics, Gene Expression Regulation drug effects, Genes, bcl-2 drug effects, Guided Tissue Regeneration, Humans, Osteoblasts cytology, Osteoblasts drug effects, Osteoblasts metabolism, Osteoblasts physiology, Polyvinyls pharmacology, Titanium pharmacology, Barium Compounds chemistry, Bone and Bones drug effects, Membranes, Artificial, Polyvinyls chemistry, Titanium chemistry, Tooth Socket cytology
- Abstract
This study investigated the response of human alveolar bone-derived cells to a novel poly(vinylidene fluoride-trifluoroethylene)/barium titanate (P(VDF-TrFE)/BT) membrane. Osteoblastic cells were cultured in osteogenic conditions either on P(VDF-TrFE)/BT or polytetrafluoroethylene (PTFE) for up to 14 days. At 7 and 14 days, the mRNA expression of Runt-related transcription factor 2 (RUNX2), Type I collagen (COL I), Osteopontin (OPN), Alkaline phosphatase (ALP), Bone sialoprotein (BSP), and Osteocalcin (OC), key markers of the osteoblastic phenotype, and of Bcl2-associated X protein (Bax), B-cell CLL/lymphoma 2 (Bcl-2), and Survivin (SUR), associated with the control of the apoptotic cell death, was assayed by real-time PCR. In situ ALP activity was qualitatively evaluated by means of Fast red staining. Surface characterization was also qualitatively and quantitatively assayed in terms of topography, roughness, and wettability. Cells grown on P(VDF-TrFE)/BT exhibited a significantly higher mRNA expression for all markers compared to the ones on PTFE, except for Bcl-2, which was not detected for both groups. Additionally, Fast red staining was noticeably stronger in cultures on P(VDF-TrFE)/BT at 7 and 14 days. At micron- and submicron scale, SEM images and roughness analysis revealed that PTFE and P(VDF-TrFE)/BT exhibited a smooth topography and a similar roughness, respectively. PTFE membrane displayed higher contact angles compared with P(VDF-TrFE)/BT, as indicated by wettability assay. The novel P(VDF-TrFE)/BT membrane supports the acquisition of the osteoblastic phenotype in vitro, while up-regulating the expression of apoptotic markers. Further in vivo experiments should be carried out to confirm the capacity of P(VDF-TrFE)/BT membrane in promoting bone formation in guided bone regeneration.
- Published
- 2011
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9. In vitro biocompatibility of poly(vinylidene fluoride-trifluoroethylene)/barium titanate composite using cultures of human periodontal ligament fibroblasts and keratinocytes.
- Author
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Teixeira LN, Crippa GE, Trabuco AC, Gimenes R, Zaghete MA, Palioto DB, de Oliveira PT, Rosa AL, and Beloti MM
- Subjects
- Cells, Cultured, Fibroblasts cytology, Humans, Keratinocytes cytology, Materials Testing, Periodontal Ligament cytology, Barium Compounds chemistry, Biocompatible Materials chemistry, Fibroblasts physiology, Guided Tissue Regeneration, Periodontal methods, Hydrocarbons, Fluorinated chemistry, Keratinocytes physiology, Periodontal Ligament physiology, Polyvinyls chemistry, Titanium chemistry
- Abstract
The aim of this work was to evaluate the biocompatibility of poly(vinylidene fluoride-trifluoroethylene)/barium titanate (P(VDF-TrFE)/BT) membrane to be used in guided tissue regeneration (GTR). Fibroblasts from human periodontal ligament (hPDLF) and keratinocytes (SCC9) were plated on P(VDF-TrFE)/BT and polytetrafluorethylene membranes at a cell density of 20,000 cells well(-1) and cultured for up to 21 days. Cell morphology, adhesion and proliferation were evaluated in hPDLF and keratinocytes, while total protein content and alkaline phosphatase (ALP) activity were assayed only for hPDLF. Using a higher cell density, real-time polymerase chain reaction (PCR) was performed to assess the expression of typical genes of hPDLF, such as periostin, PDLs17, S100A4 and fibromodulin, and key phenotypic markers of keratinocytes, including involucrin, keratins 1, 10 and 14. Expression of the apoptotic genes bax, bcl-2 and survivin was evaluated for both cultures. hPDLF adhered and spread more on P(VDF-TrFE)/BT, whereas keratinocytes showed a round shape on both membranes. hPDLF adhesion was greater on P(VDF-TrFE)/BT at 2 and 4h, while keratinocyte adhesion was similar for both membranes. Whereas proliferation was significantly higher for hPDLF on P(VDF-TrFE)/BT at days 1 and 7, no signs of keratinocyte proliferation could be noticed for both membranes. Total protein content was greater on P(VDF-TrFE)/BT at 7, 14 and 21 days, and higher levels of ALP activity were observed on P(VDF-TrFE)/BT at 21 days. Real-time PCR revealed higher expression of phenotypic markers of hPDLF and keratinocytes as well as greater expression of apoptotic genes in cultures grown on P(VDF-TrFE)/BT. These results indicate that, by favoring hPDLF adhesion, spreading, proliferation and typical mRNA expression, P(VDF-TrFE)/BT membrane should be considered an advantageous alternative for GTR., (Copyright 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2010
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10. Effects of low-level laser therapy on human osteoblastic cells grown on titanium.
- Author
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Petri AD, Teixeira LN, Crippa GE, Beloti MM, de Oliveira PT, and Rosa AL
- Subjects
- Alkaline Phosphatase biosynthesis, Alkaline Phosphatase genetics, Analysis of Variance, Bone Morphogenetic Protein 7 biosynthesis, Bone Morphogenetic Protein 7 genetics, Cells, Cultured radiation effects, Collagen Type I biosynthesis, Collagen Type I genetics, Core Binding Factor Alpha 1 Subunit biosynthesis, Core Binding Factor Alpha 1 Subunit genetics, Humans, Integrin-Binding Sialoprotein biosynthesis, Integrin-Binding Sialoprotein genetics, Intercellular Adhesion Molecule-1 biosynthesis, Intercellular Adhesion Molecule-1 genetics, Lasers, Semiconductor therapeutic use, Osteoblasts metabolism, Osteocalcin biosynthesis, Osteocalcin genetics, Osteopontin biosynthesis, Osteopontin genetics, Osteoprotegerin biosynthesis, Osteoprotegerin genetics, RANK Ligand biosynthesis, RANK Ligand genetics, Statistics, Nonparametric, Titanium, Bone Matrix growth & development, Gene Expression radiation effects, Low-Level Light Therapy, Osseointegration radiation effects, Osteoblasts radiation effects
- Abstract
The aim of this study was to investigate the effects of low-level laser therapy (LLLT) by using gallium aluminum arsenide (GaAlAs) diode laser on human osteoblastic cells grown on titanium (Ti). Osteoblastic cells were obtained by enzymatic digestion of human alveolar bone and cultured on Ti discs for up to 17 days. Cells were exposed to LLLT at 3 J/cm2 (wavelength of 780 nm) at days 3 and 7 and non-irradiated cultures were used as control. LLLT treatment did not influence culture growth, ALP activity, and mineralized matrix formation. Analysis of cultures by epifluorescence microscopy revealed an area without cells in LLLT treated cultures, which was repopulated latter with proliferative and less differentiated cells. Gene expression of ALP, OC, BSP, and BMP-7 was higher in LLLT treated cultures, while Runx2, OPN, and OPG were lower. These results indicate that LLLT modulates cell responses in a complex way stimulating osteoblastic differentiation, which suggests possible benefits on implant osseointegration despite a transient deleterious effect immediately after laser irradiation.
- Published
- 2010
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11. In vitro proliferation and osteoblastic phenotype expression of cells derived from human vertebral lamina and iliac crest.
- Author
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Defino HL, da Silva Herrero CF, Crippa GE, Bellesini LS, Beloti MM, and Rosa AL
- Subjects
- Biomarkers analysis, Biomarkers metabolism, Bone Matrix chemistry, Bone Matrix metabolism, Bone Regeneration physiology, Bone Transplantation methods, Calcification, Physiologic physiology, Cell Culture Techniques, Cells, Cultured, Gene Expression Regulation physiology, Humans, Ilium physiology, Osteoblasts physiology, Osteogenesis physiology, Phenotype, Spinal Fusion methods, Spine physiology, Cell Differentiation physiology, Cell Proliferation, Ilium cytology, Osteoblasts cytology, Spine cytology
- Abstract
Study Design: Osteoblastic cells derived from vertebral lamina and iliac crest were isolated and cultured under the same conditions (osteogenic medium, pH, temperature, and CO2 levels)., Objective: To compare proliferation and expression of osteoblastic phenotype of cells derived from vertebral lamina and iliac grafting., Summary of Background Data: Many factors play a role in the success of bone graft in spinal fusion including osteoblastic cell population. Two common sources of graft are vertebral lamina and iliac crest, however, differences in proliferation and osteoblastic phenotype expression between cells from these sites have not been investigated., Methods: Cells obtained from cancellous bone of both vertebral lamina and iliac crest were cultured and proliferation was evaluated by direct cell counting and viability detected by Trypan blue. Alkaline phosphatase (ALP) activity was evaluated by thymolphthalein release from thymolphthalein monophosphate and matrix mineralization by staining with alizarin red S. Gene expression of ALP, osteocalcin, runt-related transcription factor 2, Msh homeobox 2, bone morphogenetic protein 7, intercellular adhesion molecule 1 precursor, osteoprotegerin, and receptor activator of NF-kB ligand was analyzed by real-time PCR. All comparisons were donor-matched., Results: Proliferation was greater at days 7 and 10 in cells from vertebral lamina compared with ones from iliac crest without difference in cell viability. ALP activity was higher in cells from vertebral lamina compared with cells from iliac crest at days 7 and 10. At 21 days, mineralized matrix was higher in cells derived from vertebral lamina than from iliac crest. At day 7, gene expression of ALP, osteocalcin, runt-related transcription factor 2, Msh homeobox 2, bone morphogenetic protein 7, intercellular adhesion molecule 1 precursor, receptor activator of NF-kB ligand, and osteoprotegerin was higher in cells derived from vertebral lamina compared with iliac crest., Conclusion: Cell proliferation and osteoblastic phenotype development in cells derived from cancellous bone were more exuberant in cultures of vertebral lamina than of iliac crest.
- Published
- 2009
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12. Human alveolar bone cell proliferation, expression of osteoblastic phenotype, and matrix mineralization on porous titanium produced by powder metallurgy.
- Author
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Rosa AL, Crippa GE, de Oliveira PT, Taba M Jr, Lefebvre LP, and Beloti MM
- Subjects
- Alkaline Phosphatase drug effects, Alkaline Phosphatase metabolism, Alveolar Process cytology, Alveolar Process physiology, Analysis of Variance, Biocompatible Materials chemistry, Bone Matrix, Calcification, Physiologic physiology, Cell Adhesion drug effects, Cell Adhesion physiology, Cell Proliferation drug effects, Gene Expression Regulation drug effects, Gene Expression Regulation physiology, Humans, Metallurgy, Osseointegration drug effects, Osseointegration physiology, Osteoblasts cytology, Osteoblasts physiology, Osteogenesis physiology, Porosity, Titanium chemistry, Biocompatible Materials pharmacology, Calcification, Physiologic drug effects, Osteoblasts drug effects, Osteogenesis drug effects, Titanium pharmacology
- Abstract
Objective: This study aimed at investigating the influence of the porous titanium (Ti) structure on the osteogenic cell behaviour., Materials and Methods: Porous Ti discs were fabricated by the powder metallurgy process with the pore size typically between 50 and 400 microm and a porosity of 60%. Osteogenic cells obtained from human alveolar bone were cultured until subconfluence and subcultured on dense Ti (control) and porous Ti for periods of up to 17 days., Results: Cultures grown on porous Ti exhibited increased cell proliferation and total protein content, and lower levels of alkaline phosphatase (ALP) activity than on dense Ti. In general, gene expression of osteoblastic markers-runt-related transcription factor 2, collagen type I, alkaline phosphatase, bone morphogenetic protein-7, and osteocalcin was lower at day 7 and higher at day 17 in cultures grown on porous Ti compared with dense Ti, a finding consistent with the enhanced growth rate for such cultures. The amount of mineralized matrix was greater on porous Ti compared with the dense one., Conclusion: These results indicate that the porous Ti is an appropriate substrate for osteogenic cell adhesion, proliferation, and production of a mineralized matrix. Because of the three-dimensional environment it provides, porous Ti should be considered an advantageous substrate for promoting desirable implant surface-bone interactions.
- Published
- 2009
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13. Development of the osteoblastic phenotype in human alveolar bone-derived cells grown on a collagen type I-coated titanium surface.
- Author
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de Assis AF, Beloti MM, Crippa GE, de Oliveira PT, Morra M, and Rosa AL
- Subjects
- Alveolar Process metabolism, Bone Matrix metabolism, Cell Adhesion physiology, Cell Culture Techniques, Cell Differentiation physiology, Cell Proliferation, Humans, Osseointegration physiology, Osteoblasts metabolism, RNA, Messenger analysis, Surface Properties, Titanium, Alveolar Process cytology, Calcification, Physiologic physiology, Coated Materials, Biocompatible metabolism, Collagen Type I metabolism, Osteoblasts cytology
- Abstract
Objective: The aim of this study was to evaluate the development of the osteoblastic phenotype in human alveolar bone-derived cells grown on collagen type I-coated titanium (Ti) surface (Col-Ti) obtained by plasma deposition acrylic acid grafting compared with machined Ti (M-Ti)., Material and Methods: Osteoblastic cells were cultured until subconfluence and subcultured on Col-Ti and M-Ti for periods of up to 21 days., Results: Cultures grown on Col-Ti and M-Ti exhibited similar cell morphology. Cell adhesion, total protein content, and alkaline phosphatase (ALP) activity were not affected by Ti surface modification in all evaluated periods. Growth analyses indicated that there were significantly more cells in cultures grown on Col-Ti at day 3. Runt-related transcription factor 2 (Runx2), osteopontin (OPN), and osteoprotegerin (OPG) mRNA expression of cells subcultured on Col-Ti was higher, whereas collagen type I (COL) was lower compared with M-Ti. Ti surface modification neither affected the osteocalcin (OC), ALP and receptor activator of NF-kappaB ligand (RANKL) mRNA expression nor the calcium content extracted from mineralized matrix., Conclusions: These results demonstrated that Col-Ti favours cell growth during the proliferative phase (day 3) and osteoblastic differentiation, as demonstrated by changes in mRNA expression profile during the matrix mineralization phase (day 14), suggesting that this Ti surface modification may affect the processes of bone healing and remodelling.
- Published
- 2009
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14. The effect of TAK-778 on gene expression of osteoblastic cells is mediated through estrogen receptor.
- Author
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Bellesini LS, Beloti MM, Crippa GE, Bombonato-Prado KF, Junta CM, Marques MM, Passos GA, and Rosa AL
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- Bone and Bones drug effects, Bone and Bones metabolism, Humans, Oligonucleotide Array Sequence Analysis, Osteogenesis drug effects, Reverse Transcriptase Polymerase Chain Reaction, Benzothiepins pharmacology, Gene Expression Regulation drug effects, Osteoblasts drug effects, Osteoblasts metabolism, Receptors, Estrogen metabolism
- Abstract
This study evaluated the effect of TAK-778 [(2R, 4S)-(-)-N-(4-diethoxyphosphorylmethylphenyl)-1,2,4,5-tetrahydro-4-methyl-7,8-methylenedioxy-5-oxo-3-benzothiepin-2-carboxamide)] on in vitro osteogenic events and on gene expression of osteoblastic cells derived from human alveolar bone and the participation of estrogen receptors (ERs) on such effect. Osteoblastic cells were subcultured, with or without TAK-778 (10(-5) M), to evaluate cell growth and viability, total protein content, and alkaline phosphatase (ALP) activity at 7, 14, and 21 days; bone-like formation at 21 days; and gene expression, using cDNA microarray, at 7 days. Also, osteoblastic cells were exposed to TAK-778 (10(-5) M) combined to ICI182,780, a nonspecific ER antagonist (10(-6) M), and gene expression was evaluated by real-time polymerase chain reaction (PCR) at 7 days. TAK-778 induced a reduction in culture growth and an increase in cell synthesis, ALP activity, and bone-like formation. The cDNA microarray showed genes associated with cell adhesion and differentiation, skeletal development, ossification, and transforming growth factor-beta receptor signaling pathway, with a tendency to be higher expressed in cells exposed to TAK-778. The gene expression of ALP, osteocalcin, Msh homeobox 2, receptor activator of NF-kappa B ligand, and intercellular adhesion molecule 1 was increased by TAK-778 as demonstrated by real-time PCR, and this effect was antagonized by ICI182,780. The present results demonstrated that TAK-778 acts at a transcriptional level to enhance the in vitro osteogenic process and that its effect on gene expression of osteoblastic cells is mediated, at least partially, through ERs. Based on these findings, TAK-778 could be considered in the treatment of bone metabolic disorders.
- Published
- 2009
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15. Evidence of the presence of T helper type 17 cells in chronic lesions of human periodontal disease.
- Author
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Cardoso CR, Garlet GP, Crippa GE, Rosa AL, Júnior WM, Rossi MA, and Silva JS
- Subjects
- Adult, Alveolar Bone Loss metabolism, Case-Control Studies, Female, Fluorescent Antibody Technique, Gene Expression, Humans, Immunohistochemistry, Interleukin-1beta biosynthesis, Interleukin-23 biosynthesis, Interleukin-6 biosynthesis, Male, Microscopy, Confocal, RANK Ligand biosynthesis, RNA, Messenger biosynthesis, T-Lymphocytes, Helper-Inducer metabolism, Transforming Growth Factor beta biosynthesis, Alveolar Bone Loss immunology, Chronic Periodontitis immunology, Interleukin-17 biosynthesis, T-Lymphocytes, Helper-Inducer immunology
- Abstract
Introduction: Periodontal disease is a chronic inflammation of the attachment structures of the teeth, triggered by potentially hazardous microorganisms and the consequent immune-inflammatory responses. In humans, the T helper type 17 (Th17) lineage, characterized by interleukin-17 (IL-17) production, develops under transforming growth factor-beta (TGF-beta), IL-1beta, and IL-6 signaling, while its pool is maintained by IL-23. Although this subset of cells has been implicated in various autoimmune, inflammatory, and bone-destructive conditions, the exact role of T lymphocytes in chronic periodontitis is still controversial. Therefore, in this study we investigated the presence of Th17 cells in human periodontal disease., Methods: Gingival and alveolar bone samples from healthy patients and patients with chronic periodontitis were collected and used for the subsequent assays. The messenger RNA expression for the cytokines IL-17, TGF-beta, IL-1beta, IL-6, and IL-23 in gingiva or IL-17 and receptor activator for nuclear factor-kappaB ligand in alveolar bone was evaluated by real-time polymerase chain reaction. The production of IL-17, TGF-beta, IL-1beta, IL-6, and IL-23 proteins was evaluated by immunohistochemistry and the presence of Th17 cells in the inflamed gingiva was confirmed by immunofluorescence confocal microscopy for CD4 and IL-17 colocalization., Results: Our data demonstrated elevated levels of IL-17, TGF-beta, IL-1beta, IL-6, and IL-23 messenger RNA and protein in diseased tissues as well as the presence of Th17 cells in gingiva from patients with periodontitis. Moreover, IL-17 and the bone resorption factor RANKL were abundantly expressed in the alveolar bone of diseased patients, in contrast to low detection in controls., Conclusion: These results provided strong evidence for the presence of Th17 cells in the sites of chronic inflammation in human periodontal disease.
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- 2009
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16. Effects of a mixture of growth factors and proteins on the development of the osteogenic phenotype in human alveolar bone cell cultures.
- Author
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de Oliveira PT, de Oliva MA, Maximiano WM, Sebastião KE, Crippa GE, Ciancaglini P, Beloti MM, Nanci A, and Rosa AL
- Subjects
- Alkaline Phosphatase biosynthesis, Alkaline Phosphatase genetics, Animals, Becaplermin, Blood Platelets metabolism, Cell Survival drug effects, Cells, Cultured, Core Binding Factor Alpha 1 Subunit biosynthesis, Core Binding Factor Alpha 1 Subunit genetics, Culture Media, Fibronectins pharmacology, Humans, Osteoblasts physiology, Osteogenesis, Phenotype, Platelet-Derived Growth Factor pharmacology, Proto-Oncogene Proteins c-sis, RNA, Messenger biosynthesis, Rats, Recombinant Proteins pharmacology, Reverse Transcriptase Polymerase Chain Reaction, Serum Albumin pharmacology, Thrombospondins pharmacology, Transforming Growth Factors pharmacology, Blood Proteins pharmacology, Intercellular Signaling Peptides and Proteins pharmacology, Osteoblasts drug effects
- Abstract
Strategies to promote bone repair have included exposure of cells to growth factor (GF) preparations from blood that generally include proteins as part of a complex mixture. This study aimed to evaluate the effects of such a mixture on different parameters of the development of the osteogenic phenotype in vitro. Osteoblastic cells were obtained by enzymatic digestion of human alveolar bone and cultured under standard osteogenic conditions until subconfluence. They were subcultured on Thermanox coverslips up to 14 days. Treated cultures were exposed during the first 7 days to osteogenic medium supplemented with a GFs + proteins mixture containing the major components found in platelet extracts [platelet-derived growth factor-BB, transforming growth factor (TGF)-beta1, TGF-beta2, albumin, fibronectin, and thrombospondin] and to osteogenic medium alone thereafter. Control cultures were exposed only to the osteogenic medium. Treated cultures exhibited a significantly higher number of adherent cells from day 4 onward and of cycling cells at days 1 and 4, weak alkaline phosphatase (ALP) labeling, and significantly decreased levels of ALP activity and mRNA expression. At day 14, no Alizarin red-stained nodular areas were detected in cultures treated with GFs + proteins. Results were confirmed in the rat calvaria-derived osteogenic cell culture model. The addition of bone morphogenetic protein 7 or growth and differentiation factor 5 to treated cultures upregulated Runx2 and ALP mRNA expression, but surprisingly, ALP activity was not restored. These results showed that a mixture of GFs + proteins affects the development of the osteogenic phenotype both in human and rat cultures, leading to an increase in the number of cells, but expressed a less differentiated state.
- Published
- 2008
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17. Effect of growth hormone on in vitro osteogenesis and gene expression of human osteoblastic cells is donor-age-dependent.
- Author
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Crippa GE, Beloti MM, Cardoso CR, Silva JS, and Rosa AL
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- Adolescent, Adult, Age Factors, Biomarkers analysis, Cells, Cultured, Humans, Middle Aged, RNA, Messenger analysis, Up-Regulation, Gene Expression Regulation drug effects, Human Growth Hormone pharmacology, Osteoblasts metabolism, Osteogenesis drug effects
- Abstract
It has been demonstrated that the effect of GH on bone tissue is reduced with aging. In this study we tested the hypothesis that the action of GH on osteoblastic cells is donor-age-dependent by investigating the effect of GH on the development of osteoblastic phenotype in cultures of cells from adolescents (13-16 years old), young adults (18-35 years old), and adults (36-49 years old). Osteoblastic cells derived from human alveolar bone were cultured with or without GH for periods of up to 21 days, and parameters of in vitro osteogenesis and gene expression of osteoblastic markers were evaluated. GH increased culture growth, collagen content and alkaline phosphatase (ALP) activity in cultures from adolescents and young adults, whereas non-significant effect was observed in cultures from adults. While GH significantly increased the bone-like formation in cultures from adolescents, a slightly effect was observed in cultures from young adults and no alteration was detected in cultures from adults. Results from real-time PCR demonstrated that GH upregulated ALP, osteocalcin, type I collagen, and Cbfa1 mRNA levels in cultures from adolescents. In addition, cultures from young adults showed higher ALP mRNA expression and the expression of all evaluated genes was not affected by GH in cultures from adults. These results indicate that the GH effect on both in vitro osteogenesis and gene expression of osteoblastic markers is donor-age-dependent, being more pronounced on cultures from adolescents., (Copyright 2007 Wiley-Liss, Inc.)
- Published
- 2008
- Full Text
- View/download PDF
18. Mechanisms involved in the pressor response to noradrenaline injection into the cingulate cortex of unanesthetized rats.
- Author
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Fernandes KB, Crippa GE, Tavares RF, Antunes-Rodrigues J, and Corrêa FM
- Subjects
- Adrenergic alpha-Antagonists pharmacology, Animals, Antidiuretic Hormone Receptor Antagonists, Arginine Vasopressin analogs & derivatives, Arginine Vasopressin pharmacology, Blood Pressure physiology, Dioxanes pharmacology, Dose-Response Relationship, Drug, Ganglionic Blockers pharmacology, Gyrus Cinguli drug effects, Heart Rate drug effects, Heart Rate physiology, Hypophysectomy, Idazoxan analogs & derivatives, Idazoxan pharmacology, Male, Mecamylamine pharmacology, Microinjections, Norepinephrine pharmacology, Phenoxybenzamine pharmacology, Rats, Rats, Wistar, Arginine Vasopressin blood, Blood Pressure drug effects, Gyrus Cinguli physiology, Norepinephrine physiology
- Abstract
The cingulate cortex (CC) is involved in cardiovascular modulation. CC electrical or chemical stimulation may evoke either pressor or depressor responses, depending on the stimulated site and experimental conditions such as anesthesia. Noradrenaline (NA) is involved in cardiovascular regulation and it is present throughout the cortex. However, there is no report on the cardiovascular effects of intracortical injections of NA. We attempted to verify the effect of NA injection into the CC and to identify possible receptor and peripheral mechanisms involved. NA injection caused pressor responses accompanied by bradycardia, in unanesthetized rats. These responses were markedly reduced under urethane anesthesia. The pressor response was blocked by intracortical pretreatment with phenoxybenzamine or the selective alpha(1)-antagonist WB4101, and it was not affected by pretreatment with the selective alpha(2)-antagonist RX821002, suggesting that alpha(1)-adrenoceptors mediate the response. The pressor response was potentiated by pretreatment with the ganglion blocker mecamylamine and it was abolished by pretreatment with the vasopressin antagonist, dTyr(CH(2)) (5)(Me)AVP or by hypophysectomy. Circulating vasopressin levels were increased after NA injection into the CC. The present results indicate that the pressor response to local injection of NA within the CC is independent of sympathetic nerve activation and is mediated by vasopressin release.
- Published
- 2003
- Full Text
- View/download PDF
19. The lateral hypothalamus is involved in the pathway mediating the hypotensive response to cingulate cortex-cholinergic stimulation.
- Author
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Pajolla GP, Crippa GE, Corrêa SA, Moreira KB, Tavares RF, and Corrêa FM
- Subjects
- Acetylcholine pharmacology, Animals, Axonal Transport drug effects, Axonal Transport physiology, Biotin administration & dosage, Dextrans administration & dosage, Fluorescent Dyes administration & dosage, Gyrus Cinguli drug effects, Hypotension chemically induced, Hypothalamic Area, Lateral drug effects, Injections, Intraventricular, Male, N-Methylaspartate toxicity, Neural Pathways drug effects, Neural Pathways physiology, Prefrontal Cortex drug effects, Rats, Rats, Wistar, Biotin analogs & derivatives, Cholinergic Agents pharmacology, Gyrus Cinguli physiology, Hypotension physiopathology, Hypothalamic Area, Lateral physiology, Prefrontal Cortex physiology
- Abstract
1. The injection of acetylcholine (ACh) into the medial prefrontal cortex (MPFC) caused marked hypotensive response in either unanesthetized or anesthetized rats. 2. The present experiment was designed to investigate anatomical connections of the ACh injection site in the MPFC with putative autonomic-related brain nuclei, as well as their possible involvement in the mediation of the hypotensive response to ACh. 3. For the above purpose, the bidirectional neuronal tracer biotinylated dextran amine (BDA) was injected into Cg1 and Cg3 areas, within the MPFC of male Wistar rats. Five days later the animals were sacrificed and brain slices were processed and analyzed to determine neuronal projections efferent from as well afferent to the MPFC. 4. Neuronal staining was more prominent in regions ipsilateral to the BDA injection site. Prominent efferent projections of the MPFC were observed in the contralateral MPFC: ipsi- and contralateral amygdala and hypothalamus; ipsilateral septal area, diagonal band, and zona incerta. 5. Similar but not equal patterns of neuronal labeling were observed when BDA injections were performed within the two adjacent MPFC areas. BDA injections centered in the ACh injection site in the Cg3 area caused strong labeling in the septal area and diagonal band as well as an overall hypothalamic labeling. Within the hypothalamus an intense cortical projection was observed in the lateral hypothalamus (LH). BDA injections into the Cg1 area caused a more evident labeling of the amygdaloid complex. 6. Neuronal cell bodies were evident throughout the MPFC as well as in the sensory-motor cortex when BDA was injected into the LH, thus indicating a massive ipsilateral cortical projection from the Cg3 to the LH. 7. Bilateral NMDA-induced lesions within the LH caused a significant attenuation of the depressor responses to ACh injection in the MPFC, whereas unilateral lesions were marginally effective. These results indicate the involvement of the LH in the mediation of the hypotensive response to ACh injection into the MPFC as well as the bilateral distribution of the hypotensive pathway.
- Published
- 2001
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- View/download PDF
20. Medial prefrontal cortex acetylcholine injection-induced hypotension: the role of hindlimb vasodilation.
- Author
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Crippa GE, Lewis SJ, Johnson AK, and Corrêa FM
- Subjects
- Animals, Blood Pressure drug effects, Gyrus Cinguli physiology, Hemodynamics drug effects, Hindlimb innervation, Hypotension physiopathology, Injections, Rats, Rats, Sprague-Dawley, Sympathectomy, Sympathetic Nervous System physiopathology, Acetylcholine pharmacology, Hindlimb blood supply, Hypotension chemically induced, Prefrontal Cortex physiology, Vasodilation
- Abstract
The injection of acetylcholine (ACh) into the cingulate region of the medial prefrontal cortex (MPFC) causes a marked fall in arterial blood pressure which is not accompanied by changes in heart rate. The purpose of the present study was to investigate the hemodynamic basis for this stimulus-induced hypotension in Sprague-Dawley rats. The study was designed to determine whether a change in the vascular resistance of hindlimb, renal or mesenteric vascular beds contributes to the fall in arterial pressure in response to ACh injection into the cingulate cortex. Miniature pulsed-Doppler flow probes were used to measure changes in regional blood flow and vascular resistance. The results indicated that the hypotensive response was largely due to a consistent and marked vasodilation in the hindlimb vascular bed. On this basis, an additional experiment was then undertaken to determine the mechanisms that contribute to hindlimb vasodilation. The effect of interrupting the autonomic innervation of one leg on the hindlimb vasodilator response was tested. Unilateral transection of the lumbar sympathetic chain attenuated the cingulate ACh-induced vasodilation in the ipsilateral, but not in the contralateral hindlimb. These results suggest that the hypotensive response to cingulate cortex-ACh injection is caused by skeletal muscle vasodilation mediated by a sympathetic chain-related vasodilator system.
- Published
- 2000
- Full Text
- View/download PDF
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