Back to Search
Start Over
Evidence of the presence of T helper type 17 cells in chronic lesions of human periodontal disease.
- Source :
-
Oral microbiology and immunology [Oral Microbiol Immunol] 2009 Feb; Vol. 24 (1), pp. 1-6. - Publication Year :
- 2009
-
Abstract
- Introduction: Periodontal disease is a chronic inflammation of the attachment structures of the teeth, triggered by potentially hazardous microorganisms and the consequent immune-inflammatory responses. In humans, the T helper type 17 (Th17) lineage, characterized by interleukin-17 (IL-17) production, develops under transforming growth factor-beta (TGF-beta), IL-1beta, and IL-6 signaling, while its pool is maintained by IL-23. Although this subset of cells has been implicated in various autoimmune, inflammatory, and bone-destructive conditions, the exact role of T lymphocytes in chronic periodontitis is still controversial. Therefore, in this study we investigated the presence of Th17 cells in human periodontal disease.<br />Methods: Gingival and alveolar bone samples from healthy patients and patients with chronic periodontitis were collected and used for the subsequent assays. The messenger RNA expression for the cytokines IL-17, TGF-beta, IL-1beta, IL-6, and IL-23 in gingiva or IL-17 and receptor activator for nuclear factor-kappaB ligand in alveolar bone was evaluated by real-time polymerase chain reaction. The production of IL-17, TGF-beta, IL-1beta, IL-6, and IL-23 proteins was evaluated by immunohistochemistry and the presence of Th17 cells in the inflamed gingiva was confirmed by immunofluorescence confocal microscopy for CD4 and IL-17 colocalization.<br />Results: Our data demonstrated elevated levels of IL-17, TGF-beta, IL-1beta, IL-6, and IL-23 messenger RNA and protein in diseased tissues as well as the presence of Th17 cells in gingiva from patients with periodontitis. Moreover, IL-17 and the bone resorption factor RANKL were abundantly expressed in the alveolar bone of diseased patients, in contrast to low detection in controls.<br />Conclusion: These results provided strong evidence for the presence of Th17 cells in the sites of chronic inflammation in human periodontal disease.
- Subjects :
- Adult
Alveolar Bone Loss metabolism
Case-Control Studies
Female
Fluorescent Antibody Technique
Gene Expression
Humans
Immunohistochemistry
Interleukin-1beta biosynthesis
Interleukin-23 biosynthesis
Interleukin-6 biosynthesis
Male
Microscopy, Confocal
RANK Ligand biosynthesis
RNA, Messenger biosynthesis
T-Lymphocytes, Helper-Inducer metabolism
Transforming Growth Factor beta biosynthesis
Alveolar Bone Loss immunology
Chronic Periodontitis immunology
Interleukin-17 biosynthesis
T-Lymphocytes, Helper-Inducer immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1399-302X
- Volume :
- 24
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Oral microbiology and immunology
- Publication Type :
- Academic Journal
- Accession number :
- 19121062
- Full Text :
- https://doi.org/10.1111/j.1399-302X.2008.00463.x