6 results on '"Clotilde Langlois"'
Search Results
2. A Dietary Supplement Containing Cinnamon, Chromium and Carnosine Decreases Fasting Plasma Glucose and Increases Lean Mass in Overweight or Obese Pre-Diabetic Subjects: A Randomized, Placebo-Controlled Trial.
- Author
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Yuejun Liu, Aurélie Cotillard, Camille Vatier, Jean-Philippe Bastard, Soraya Fellahi, Marie Stévant, Omran Allatif, Clotilde Langlois, Séverine Bieuvelet, Amandine Brochot, Angèle Guilbot, Karine Clément, and Salwa W Rizkalla
- Subjects
Medicine ,Science - Abstract
Preventing or slowing the progression of prediabetes to diabetes is a major therapeutic issue.Our aim was to evaluate the effects of 4-month treatment with a dietary supplement containing cinnamon, chromium and carnosine in moderately obese or overweight pre-diabetic subjects, the primary outcome being change in fasting plasma glucose (FPG) level. Other parameters of plasma glucose homeostasis, lipid profile, adiposity and inflammatory markers were also assessed.In a randomized, double-blind, placebo-controlled study, 62 subjects with a FPG level ranging from 5.55 to 7 mmol/L and a body mass index ≥ 25 kg/m(2), unwilling to change their dietary and physical activity habits, were allocated to receive a 4-month treatment with either 1.2 g/day of the dietary supplement or placebo. Patients were followed up until 6 months post-randomization.Four-month treatment with the dietary supplement decreased FPG compared to placebo (-0.24 ± 0.50 vs +0.12 ± 0.59 mmol/L, respectively, p = 0.02), without detectable significant changes in HbA1c. Insulin sensitivity markers, plasma insulin, plasma lipids and inflammatory markers did not differ between the treatment groups. Although there were no significant differences in changes in body weight and energy or macronutrient intakes between the two groups, fat-free mass (%) increased with the dietary supplement compared to placebo (p = 0.02). Subjects with a higher FPG level and a milder inflammatory state at baseline benefited most from the dietary supplement.Four-month treatment with a dietary supplement containing cinnamon, chromium and carnosine decreased FPG and increased fat-free mass in overweight or obese pre-diabetic subjects. These beneficial effects might open up new avenues in the prevention of diabetes.ClinicalTrials.gov NCT01530685.
- Published
- 2015
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3. Distinct regulation of adiponutrin/PNPLA3 gene expression by the transcription factors ChREBP and SREBP1c in mouse and human hepatocytes
- Author
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Marthe Moldes, Fabienne Foufelle, Françoise Lasnier, Catherine Postic, Isabelle Dugail, Jean Girard, Céline Dubuquoy, Anne-Françoise Burnol, Clotilde Langlois, and Céline Robichon
- Subjects
education.field_of_study ,Reporter gene ,Hepatology ,biology ,Response element ,Molecular biology ,Fatty acid synthase ,Gene expression ,Transcriptional regulation ,biology.protein ,Adiponutrin ,education ,Carbohydrate-responsive element-binding protein ,Transcription factor - Abstract
Background & Aims The adiponutrin/PNPLA3 (patatin-like phospholipase domain-containing protein 3) variant I148M has recently emerged as an important marker of human fatty liver disease. In order to understand the role of the adiponutrin/PNPLA3 protein, we investigated the regulation of its expression in both human and mouse hepatocytes. Methods Adiponutrin/PNPLA3 and lipogenic enzyme expression was determined by real-time PCR analysis in a wide panel of analysis in vivo in the mouse liver and in vitro in murine hepatocytes and human hepatocyte cell lines infected with ChREBP or SREBP1c-expressing adenoviruses. Results We show that in the mouse liver, adiponutrin/PNPLA3 gene expression is under the direct transcriptional control of ChREBP (carbohydrate-response element-binding protein) and SREBP1c (sterol regulatory element binding protein1c) in response to glucose and insulin, respectively. In silico analysis revealed the presence of a ChoRE (carbohydrate response element) and of a SRE (sterol response element) binding site on the mouse adiponutrin/PNPLA3 gene promoter. Point mutation analysis in reporter gene assays identified the functional response of these two binding sites in the mouse adiponutrin/PNPLA3 promoter. In contrast, in human immortalized hepatocytes and in HepG2 hepatoma cells, only SREBP1c was able to induce adiponutrin/PNPLA3 expression, whereas ChREBP was unable to modulate its expression. Conclusions All together, our results suggest that adiponutrin/PNPLA3 is regulated by two key factors of the glycolytic and lipogenic pathways, raising the question of its implication in the metabolism of carbohydrates and lipids.
- Published
- 2011
4. Les protéines à domaine patatine
- Author
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Sylvain Baulande and Clotilde Langlois
- Subjects
Sequence alignment ,Lipid metabolism ,Genomics ,General Medicine ,Computational biology ,Patatin ,Biology ,Functional genomics ,Gene ,General Biochemistry, Genetics and Molecular Biology ,DNA sequencing ,Conserved sequence - Abstract
Genome sequencing technologies led to tremendous breakthrough in biology uncovering numerous genes unknown so far and thus opening the field of deep investigations to understand their associated biological functions. As a matter of fact, functional genomics have been progressively replacing sequence genomics with as a main objective to yield insight into cellular physiology. Recently, an emerging group of genes coding for proteins bearing a common domain termed patatin (PNPLA domain) have been discovered. Members of this new enzymatic family displaying lipase and transacylase properties appeared to have major roles in the regulation of lipid metabolism. The aim of this review is to make an overview on the latest discoveries concerning this new family of proteins and their relationship with lipid metabolism, physiology of mammals and their potential involvement in human pathology.
- Published
- 2010
5. [Proteins sharing PNPLA domain, a new family of enzymes regulating lipid metabolism]
- Author
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Sylvain, Baulande and Clotilde, Langlois
- Subjects
Mammals ,Saccharomyces cerevisiae Proteins ,Sequence Homology, Amino Acid ,Arabidopsis Proteins ,Escherichia coli Proteins ,Lipolysis ,Molecular Sequence Data ,Lipase ,Lipid Metabolism ,Mice ,Species Specificity ,Catalytic Domain ,Multigene Family ,Phospholipases A2, Calcium-Independent ,Animals ,Drosophila Proteins ,Humans ,Amino Acid Sequence ,Caenorhabditis elegans Proteins ,Carboxylic Ester Hydrolases ,Sequence Alignment ,Conserved Sequence ,Phylogeny ,Plant Proteins - Abstract
Genome sequencing technologies led to tremendous breakthrough in biology uncovering numerous genes unknown so far and thus opening the field of deep investigations to understand their associated biological functions. As a matter of fact, functional genomics have been progressively replacing sequence genomics with as a main objective to yield insight into cellular physiology. Recently, an emerging group of genes coding for proteins bearing a common domain termed patatin (PNPLA domain) have been discovered. Members of this new enzymatic family displaying lipase and transacylase properties appeared to have major roles in the regulation of lipid metabolism. The aim of this review is to make an overview on the latest discoveries concerning this new family of proteins and their relationship with lipid metabolism, physiology of mammals and their potential involvement in human pathology.
- Published
- 2010
6. A Dietary Supplement Containing Cinnamon, Chromium and Carnosine Decreases Fasting Plasma Glucose and Increases Lean Mass in Overweight or Obese Pre-Diabetic Subjects: A Randomized, Placebo-Controlled Trial
- Author
-
Séverine Bieuvelet, Salwa W. Rizkalla, Camille Vatier, Aurélie Cotillard, Marie Stévant, Jean-Philippe Bastard, Karine Clément, Amandine Brochot, Angèle Guilbot, Yuejun Liu, Omran Allatif, Clotilde Langlois, Soraya Fellahi, Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Institute of cardiometabolism and nutrition (ICAN), Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Centre de Recherche Saint-Antoine (UMRS893), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Tenon [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), AdipoPhYt, Groupe PiLeJe, Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Research Unit on Cardiovascular and Metabolic Diseases (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), and HAL-UPMC, Gestionnaire
- Subjects
Adult ,Blood Glucose ,Chromium ,Male ,medicine.medical_specialty ,Cinnamomum zeylanicum ,Placebo-controlled study ,lcsh:Medicine ,Overweight ,Placebo ,Placebos ,Prediabetic State ,Double-Blind Method ,Internal medicine ,Diabetes mellitus ,medicine ,Humans ,Obesity ,Prediabetes ,lcsh:Science ,Aged ,2. Zero hunger ,[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology ,Multidisciplinary ,medicine.diagnostic_test ,Plant Extracts ,business.industry ,Carnosine ,Muscles ,lcsh:R ,Correction ,Fasting ,Middle Aged ,medicine.disease ,3. Good health ,Endocrinology ,Diabetes Mellitus, Type 2 ,Dietary Supplements ,Body Composition ,Lean body mass ,lcsh:Q ,Female ,medicine.symptom ,business ,Lipid profile ,Body mass index ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology ,Research Article - Abstract
Background Preventing or slowing the progression of prediabetes to diabetes is a major therapeutic issue. Objectives Our aim was to evaluate the effects of 4-month treatment with a dietary supplement containing cinnamon, chromium and carnosine in moderately obese or overweight pre-diabetic subjects, the primary outcome being change in fasting plasma glucose (FPG) level. Other parameters of plasma glucose homeostasis, lipid profile, adiposity and inflammatory markers were also assessed. Methods In a randomized, double-blind, placebo-controlled study, 62 subjects with a FPG level ranging from 5.55 to 7 mmol/L and a body mass index ≥25 kg/m2, unwilling to change their dietary and physical activity habits, were allocated to receive a 4-month treatment with either 1.2 g/day of the dietary supplement or placebo. Patients were followed up until 6 months post-randomization. Results Four-month treatment with the dietary supplement decreased FPG compared to placebo (-0.24±0.50 vs +0.12±0.59 mmol/L, respectively, p = 0.02), without detectable significant changes in HbA1c. Insulin sensitivity markers, plasma insulin, plasma lipids and inflammatory markers did not differ between the treatment groups. Although there were no significant differences in changes in body weight and energy or macronutrient intakes between the two groups, fat-free mass (%) increased with the dietary supplement compared to placebo (p = 0.02). Subjects with a higher FPG level and a milder inflammatory state at baseline benefited most from the dietary supplement. Conclusions Four-month treatment with a dietary supplement containing cinnamon, chromium and carnosine decreased FPG and increased fat-free mass in overweight or obese pre-diabetic subjects. These beneficial effects might open up new avenues in the prevention of diabetes. Trial Registration ClinicalTrials.gov NCT01530685
- Published
- 2015
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