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6. Interest of cytology combined with Xpert®HPV and Anyplex®II HPV28 Detection human papillomavirus (HPV) typing: differential profiles of anal and cervical HPV lesions in HIV‐infected patients on antiretroviral therapy

Author

Nassereddine, H, primary, Charpentier, C, additional, Bucau, M, additional, Joly, V, additional, Bienvenu, L, additional, Davitian, C, additional, Abramowitz, L, additional, Benabderrahmane, D, additional, Kotelevets, L, additional, Chastre, E, additional, Lehy, T, additional, and Walker, F, additional

Published
2018
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8. Functional Expression of Receptors for Calcitonin Gene-Related Peptide, Calcitonin, and Vasoactive Intestinal Peptide in the Human Thymus and Thymomas from Myasthenia Gravis Patients

Author

Marie J, Wakkach A, Coudray A, Chastre E, Sonia Berrih-Aknin, and Gespach C

Subjects
Adult, Calcitonin, Receptors, Neuropeptide, Adolescent, Thymoma, Calcitonin Gene-Related Peptide, Immunology, Cell Culture Techniques, Simian virus 40, Thymus Gland, Myasthenia Gravis, Humans, Immunology and Allergy, Receptors, Cholinergic, Antigens, Viral, Tumor, Child, Cell Line, Transformed, Reverse Transcriptase Polymerase Chain Reaction, Infant, Epithelial Cells, Middle Aged, Receptors, Calcitonin, Cell Transformation, Viral, Flow Cytometry, Immunohistochemistry, Blotting, Southern, Gene Expression Regulation, Child, Preschool, Receptors, Vasoactive Intestinal Peptide, Receptors, Calcitonin Gene-Related Peptide, Vasoactive Intestinal Peptide
Abstract

The molecular and functional expression of serpentine membrane receptors for vasoactive intestinal peptide (VIP), calcitonin gene-related peptide (CGRP), and calcitonin (CT) were characterized in human thymus and thymomas from myasthenia gravis (MG) patients and thymic epithelial cells either in primary culture (PTEC) or transformed by the siman virus 40 large T (SV40LT) oncogene (LT-TEC). Using RT-PCR combined with Southern analysis, we identified the PCR products corresponding to the receptor (-R) transcripts for VIP, CGRP, and CT in thymus from control subjects and MG patients with either hyperplasia or thymoma. Similar expressions of the VIP- and CGRP-R transcripts were observed in PTEC, whereas the CT-R message was not detected. In LT-TEC, the signals for VIP-R, CGRP-R, and CT-R transcripts were seen with a lower intensity than those in control and MG thymus. In agreement with our molecular analysis, 1) VIP was the most potent peptide among VIP-related peptides (VIP > PACAP > PHM > PHV) to stimulate cAMP production through specific type 1 VIP receptors in both PTEC and LT-TEC; 2) cAMP generation was induced by CGRP in PTEC and by CT in LT-TEC; 3) in frozen thymic sections and by flow cytometry, type 1 VIP-R, CGRP-R, and CT-R were localized in epithelial cells; and 4) in parallel, the transcription of the acetylcholine receptor α subunit (the main autoantigen in MG) was induced by CGRP and CT in PTEC and LT-TEC, respectively. Our data suggest that the neuroendocrine peptides VIP, CGRP, and CT may exert functional roles during MG and malignant transformation of the human thymus.

Published
1999

13. Interest of cytology combined with Xpert®HPV and Anyplex®II HPV28 Detection human papillomavirus (HPV) typing: differential profiles of anal and cervical HPV lesions in HIV‐infected patients on antiretroviral therapy.

Author

Nassereddine, H, Charpentier, C, Bucau, M, Joly, V, Bienvenu, L, Davitian, C, Abramowitz, L, Benabderrahmane, D, Kotelevets, L, Chastre, E, Lehy, T, and Walker, F

Subjects
PAPILLOMAVIRUS disease diagnosis, ANTIRETROVIRAL agents, ANUS, BIOLOGICAL assay, COMBINATION drug therapy, COLPOSCOPY, CYTOLOGY, EPITHELIAL cells, HIV infections, HIV-positive persons, PAP test, DISEASE prevalence, SEROTYPING, MEN who have sex with men, MIXED infections
Abstract

Objectives: The aim of the study was to assess the interest to combine cytological examination and human papillomavirus (HPV) typing of anal and cervical Papanicolaou (Pap) smears of HIV‐infected patients on combination antiretroviral therapy (cART), to evaluate whether differences in prevalence exist between anal and cervical squamous intraepithelial lesions in patients with high‐risk oncogenic HPV infection. Methods: Anal and/or cervical Pap smears were obtained by anoscopy and/or colposcopy in 238 subjects recruited consecutively in 2015: anal smears were obtained from 48 male and female patients [42 men; 35 men who have sex with men (MSM)] and cervical smears from 190 female patients. Cytological Bethesda classification was coupled with HPV typing. HPV typing was performed, on the same smears, using the Xpert®HPV Assay, which detects only high‐risk HPV (hrHPV), and the Anyplex®II HPV28 Detection assay, which detects hrHPV and low‐risk (lr) HPV. Results: Our data showed clear‐cut differences between the anal and cervical samples. Compared with the cervical samples, the anal samples exhibited (1) more numerous cytological lesions, which were histologically proven; (2) a higher hrHPV infection prevalence; (3) a higher prevalence of multiple hrHPV coinfections whatever HPV typing kit was used; (4) a predominance of HPV16 and HPV18/45 types. Overall, there was an almost perfect agreement between the two HPV typing assays (absolute agreement = 90.3%). Conclusions: Co‐testing consisting of cytology and HPV typing is a useful screening tool in the HIV‐infected population on cART. It allows detection of prevalence differences between anal and cervical HPV‐related lesions. As recently recommended, anal examination should be regularly performed especially in HIV‐infected MSM but also in HIV‐infected women with genital hrHPV lesions. [ABSTRACT FROM AUTHOR]

Published
2018
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15. Inhibition by platelet-activating factor of Src- and hepatocyte growth factor-dependent invasiveness of intestinal and kidney epithelial cells : phosphatidylinositol 3 '-kinase is a critical mediator of tumor invasion

Author

Kotelevets, Larissa, Noe, V, Bruyneel, Erik, Myssiakine, E, Chastre, E, Mareel, Marcus, and Gespach, C

Subjects
Medicine and Health Sciences
Abstract

This study was designed to characterize platelet-activating factor receptor (PAF-R) expression and function in normal and cancerous human colonic epithelial cells. PAF-R gene transcripts were analyzed by reverse transcription-polymerase chain reaction and Southern blot, using three sets of primers corresponding either to the coding region of the human PAF-R sequence (polymerase chain reaction product: 682 base pairs (bp)) or to the leukocyte-and tissue-type transcripts of 166 and 252 bp, respectively. An elongated splice variant was identified in the 5'-untranslated region of the tissue-type PAF-R transcript (334 bp) in colonic epithelial crypts and tumors. In human colonic PCmsrc cells transformed by c-src oncogene, the hepatocyte growth factor (HGF)-dependent invasiveness of collagen gels was abolished by 0.1 mu M PAF and Pestered by the PAF-R antagonists WEB2086 and SR27417. PAF blocked HGF-induced tyrosine phosphorylation of p125 focal adhesion kinase, The phosphatidylinositol 3'-kinase (PI3'-K) inhibitors wortmannin and LY294002 totally blocked the HGF-induced invasion. Similar effects were observed in ts-srcMDCK kidney epithelial cells transformed by a v-Src temperature-sensitive mutant: (i) PAF and wortmannin exerted additive inhibitory effects on Src-induced invasion and (ii) activated and dominant negative forms of p110 alpha PI3'-K, respectively, amplified and abrogated the Src- and HGF-dependent invasiveness of parental and ts-src-MDCK, cells. We also provided the first evidence for the contribution of rapamycin-insensitive, pertussis toxin-dependent G-protein pathways to the integration of the signals emerging from activated Met and PAF receptors. These results indicate that PI3'-K is a critical transducer of invasiveness and strongly suggest that PAF exerts a negative control on invasion by inhibiting this signaling pathway. A possible beneficial role of PAF analogs on tumor invasion is therefore proposed.

Published
1998

18. Characterization of binding sites for VIP related peptides and activation of adenylate cyclase in developing pancreas

Author

Le Meuth, V., Farjaudon, N., Bawab, W., Chastre, E., ROSSELIN, G., Guilloteau, Paul, Gespach, C., Laboratoire du jeune ruminant, Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, ComputingMilieux_MISCELLANEOUS, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience

Published
1991

19. Activite preferentielle du neuropeptide PACAP-38 sur les sites de liaison du 125I-VIP et de l'adenylate cyclase pendant le developpement postnatal du pancreas chez le veau

Author

Le Meuth, V., Farjaudon, N., Bawab, W., Chastre, E., Rosselin, G., Guilloteau, Paul, Gespach, C., ProdInra, Migration, Laboratoire du jeune ruminant, and Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV.GEN]Life Sciences [q-bio]/Genetics, PEPTIDE INTESTINAL VASOACTIVE, [SDV.GEN] Life Sciences [q-bio]/Genetics, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
1991

26. Progression of familial adenomatous polyposis (FAP) colonic cells after transfer of the src or polyoma middle T oncogenes: cooperation between src and HGF/Met in invasion

Author

Empereur, S, primary, Djelloul, S, additional, Di Gioia, Y, additional, Bruyneel, E, additional, Mareel, M, additional, Van Hengel, J, additional, Van Roy, F, additional, Comoglio, P, additional, Courtneidge, S, additional, Paraskeva, C, additional, Chastre, E, additional, and Gespach, C, additional

Published
1997
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30. The Somatostatin receptor subtypes SSTR2 and SSTR5 mediate the inhibition of cell proliferation

Author

Buscail, L., primary, Estève, J.-P., additional, Saint-Laurent, N., additional, Bertrand, V., additional, Chastre, E., additional, Vaillant, J.C., additional, Gespach, C., additional, Reisine, T., additional, O'Carroll, A-M., additional, Kalthof, H., additional, Bell, G.I., additional, Schally, A.V., additional, Vaysse, N., additional, and Susini, C., additional

Published
1995
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34. Oncogene-mediated propagation of tracheal epithelial cells from two cystic fibrosis fetuses with different mutations. Characterization of CFT-1 and CFT-2 cells in culture

Author

LEMNAOUAR, M., primary, CHASTRE, E., additional, PAUL, A., additional, MERGEY, M., additional, VEISSIÈRE, D., additional, CHERQUT, G., additional, BARBRY, P., additional, SIMON-BOUY, B., additional, FANEN, P., additional, GESPACH, C., additional, and PICARD, J., additional

Published
1993
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40. A new mRNA splice variant coding for the human EP3-I receptor isoform.

Author

Kotelevets, L., Foudi, N., Louedec, L., Couvelard, A., Chastre, E., and Norel, X.

Subjects
BLOOD vessels, MUCOUS membranes, PULMONARY blood vessels, MESSENGER RNA
Abstract

Abstract: The cellular localization of prostaglandin E2 receptors (EP) and their corresponding transcripts were investigated in human gastric and vascular tissues. A strong staining of the EP3 receptor on the gastric glands, mucous cells, media of the mammary and pulmonary arteries was observed by immunohistochemistry. We identified a new mRNA splice variant of the EP3 gene in human gastric fundic mucosa, mammary artery and pulmonary vessels. This EP3-Ic transcript contains exons 1, 2, 3, 5 and 6 of the EP3 gene and should be translated in the EP3-I isoform. In addition, the EP3-Ib, EP3-II, EP3-III, EP3-IV and EP3-e mRNAs were detected in these tissues. [Copyright &y& Elsevier]

Published
2007
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41. Leptin stimulates the proliferation of human colon cancer cells in vitro but does not promote the growth of colon cancer xenografts in, nude mice or intestinal tumorigenesis in Apcmin/+ mice .

Author

Aparicio, T., Kotelevets, L., Tsocas, A., Laigneau, J.-P., Sobhani, I., Chastre, E., and Lehy, T.

Subjects
COLON cancer, LEPTIN, LABORATORY mice, HORMONES, LABORATORY animals, XENOGRAFTS
Abstract

Background and aims: Leptin, the product of the ob gene, has been suggested to increase the risk of colon cancer. However, we have shown that although leptin stimulates epithelial cell proliferation it reduces the development of carcinogen induced preneoplastic lesions in the rat colon. Here, we explored the effect of leptin in vitro on proliferation of human colon cancer cells, and in vivo on the growth of HT-29 xenografts in nude mice and the development of intestinal tumours in ApcMin/+ mice. Methods: Proliferation of HT-29, LoVo, Caco2, and SW 480 cells was assessed in the absence or presence of leptin (20-500 ng/ml) by ³H-thymidine incorporation and cell count. Leptin (800 μg/kg/day) or its vehicle was delivered for four weeks to nude mice, inoculated with HT-29 cells on day 0, and for six weeks to ApcMin/+ mice. Results: Leptin dose dependently stimulated cell DNA synthesis and growth in all cell lines. In nude mice, leptin caused a 4.3-fold increase in plasma leptin levels compared with pair fed controls. This hyperleptinaemia, despite leptin receptor expression in tumours, did not induce significant variation in tumour volume or weight. Tumour Ki-67 index was even inhibited. In leptin treated ApcMin/+ mice, a 2.4- fold increase in plasma leptin levels did not modify the number, size, or distribution of intestinal adenomas compared with pair fed controls. Conclusions: Leptin acts as a growth factor on colon cancer cells in vitro but does not promote tumour growth in vivo in the two models tested. These findings do not support a pivotal role for hyperleptinaemia in intestinal carcinogenesis. [ABSTRACT FROM AUTHOR]

Published
2005
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46. Evidence for a role of Rho-like GTPases and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) in transforming growth factor beta-mediated signaling.

Author

Atfi, A, Djelloul, S, Chastre, E, Davis, R, and Gespach, C

Abstract

Transforming growth factor beta (TGF-beta) is a multifunctional factor that induces a wide variety of cellular processes which affect growth and differentiation. TGF-beta exerts its effects through a heteromeric complex between two transmembrane serine/threonine kinase receptors, the type I and type II receptors. However, the intracellular signaling pathways through which TGF-beta receptors act to generate cellular responses remain largely undefined. Here, we report that TGF-beta initiates a signaling cascade leading to stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) activation. Expression of dominant-interfering forms of various components of the SAPK/JNK signaling pathways including Rho-like GTPases, mitogen-activated protein kinase (MAPK) kinase kinase 1 (MEKK1), MAPK kinase 4 (MKK4), SAPK/JNK, and c-Jun abolishes TGF-beta-mediated signaling. Therefore, the SAPK/JNK activation contributes to TGF-beta signaling.

Published
1997

47. Pharmacological control of the histamine H2 receptor-adenylate cyclase system by famotidine and ranitidine in normal and cancerous human gastric epithelia

Author

Fagot, D., Emami, S., Chastre, E., Bawab, W., and Gespach, C.

Abstract

In human fundic glands, famotidine was about 17 times more potent than ranitidine as an inhibitor of histamine — stimulated cAMP generation. This H2-receptor antagonist had no effect on the receptoradenylate cyclase systems sensitive to PGE2, isoproterenol (β2), VIP and on forskolin-induced activation of the Gs/catalytic units of the membrane-bound enzyme prepared from human fundic glands. In the HGT-1 human gastric cancer cell line, famotidine and ranitidine showed long lasting, irreversible actions probably related to a slow rate of dissociation from the histamine H2-receptor.

Published
1988
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