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The study aimed to determine the predictors of procedural failure (coronary cannulation) in patients undergoing coronary angiography ± percutaneous coronary intervention (PCI) from the transradial (TR) approach.We conducted an observational study of 20,315 consecutive patients undergoing TR angiography between 2016 and 2020. TR failure was described as inability to cannulate the coronary arteries. Univariate and multivariate analyses were performed to determine independent predictors of TR failure.Out of the study population, TR failure was observed in 365 (1.8%) patients, out of which 281 (77%) crossed over successfully to the transfemoral (TF) route and 84 (23%) to left radial access (LRA). Unsuccessful procedures were most likely seen in patients who were elderly, female, BAME background, short stature or with a history of hypertension, diabetes, and renal disease. On regression analysis, age (OR: 1.024; 95% Cl: 1.014-1.035), female gender (OR: 0.729; 95% Cl: 0.555-0.957), BAME (OR: 0.786; 95% Cl: 0.612-0.959), height (OR: 0.988; 95% Cl: 0.977-0.999), hypertension (OR: 1.510; 95% Cl: 1.147-1.987) and RRA (OR: 1.977; 95% Cl: 1.105-3.538) were independent predictors of TR failure. On further analysis, these predictors of failure were not seen from the LRA approach.This study identifies that rates of TR failure are low and that predictors of failure differ between the RRA and LRA. The difference in predictors between the 2 routes suggests that in patients when coronary cannulation is unsuccessful via the RRA then the LRA could be considered as a second access site. Further study is needed to see if in selected patient groups the LRA could be used as the first-choice access route.

Crystal growth is commonly conceptualized with a simple structural model, the stacking of molecules or atoms represented by cubes, or sometimes spheres. The growth process is by adding single molec...

The National Center for Atmospheric Research (NCAR) developed an artificial snow-generation system designed to operate in a laboratory cold chamber for testing aircraft anti-icing fluids under controlled conditions. Flakes of ice are produced by shaving an ice cylinder with a rotating carbide bit; the resulting artificial snow is dispersed by turbulent airflows and falls approximately 2.5 m to the bottom of the device. The resulting fine ice shavings mimic snow in size, distribution, fall velocity, density, and liquid water equivalent (LWE) snowfall rate. The LWE snowfall rate can be controlled using either a mass balance or a precipitation gauge, which measures the snowfall accumulation over time, from which the computer derives the LWE rate. LWE snowfall rates are calculated every 6 s, and the rate the ice cylinder is fed into the carbide bit is continually adjusted to ensure that the LWE snowfall rate matches a user-selected value. The system has been used to generate LWE snowfall rates ranging from 0 to 10 mm h−1 at temperatures from −2 to −30°C and densities of approximately 0.1–0.5 g cm−3. Comparisons of the snow-machine fluid tests with the outdoor fluid tests have shown that the snow machine can mimic natural outdoor rates under a broad range of conditions.

Summary • We examined whether increased high temperature photosynthetic thermal tolerance (PT), reduced specific leaf area (SLA) and reduced leaf size represent correlated and convergent adaptations for recently diverged Encelia, Salvia, Atriplex and Eriogonum congeneric species pairs from contrasting thermal and water environments (the Mojave Desert and coastal California). We also studied whether variation in PT is associated with inducible small heat shock protein expression (sHsp). • Traits were measured in a common environment (CE) and in the field to partition effects of phenotypic plasticity and genetic divergence. • We found little evidence for convergent adaptation of PT (CE measurements). Field measurements revealed significant plasticity for PT, which was also associated with increased sHsp expression. Compared to coastal congeners desert species had lower SLA in the CE. These differences were magnified in the field. There was a negative correlation between SLA and PT. Desert species also tended to have smaller leaves both in the CE and in the field. • SLA and leaf size reductions represent repeated evolutionary divergences and are perhaps convergent adaptations for species radiating into the desert, while PT is highly plastic and shows little evidence for convergent adaptation in the congeneric species pairs we studied.

© 2018, Springer-Verlag GmbH Germany, part of Springer Nature. Tree crowns are spatially heterogeneous, sometimes resulting in significant variation in microclimate across the canopy, particularly with respect to temperature. Yet it is not known whether such localised temperature variation equates to intracanopy variation in leaf-level physiological thermal tolerance. Here, we studied whether microclimate variation across the canopy of a dominant desert tree equated to localised variation in leaf thermal thresholds (T 50 ) among four canopy positions: upper south, upper north, lower south, lower north. Principal component analysis was used to generate a composite climatic stress variable (C STRESS ) from canopy temperature, vapour pressure deficit, and relative humidity. We also determined the average number of days that maximum temperatures exceeded the air temperature equating to this species’ critical threshold of 49 °C (AT 49 ). To estimate how closely leaf temperatures track ambient temperature, we predicted the thermal time constant (τ) for leaves at each canopy position. We found that C STRESS and AT 49 were significantly greater in lower and north-facing positions in the canopy. Differences in wind speed with height resulted in significantly longer predicted τ for leaves positioned at lower, north-facing positions. Variation in these drivers was correlated with significantly higher T 50 for leaves in these more environmentally stressful canopy positions. Our findings suggest that this species may optimise resources to protect against thermal damage at a whole-plant level. They also indicate that, particularly in desert environments with steep intracanopy microclimatic gradients, whole-plant carbon models could substantially under- or overestimate productivity under heat stress, depending on where in the canopy T 50 is measured.

OBJECTIVES: This study aimed to determine the effect on long-term survival of using optical coherence tomography (OCT) during percutaneous coronary intervention (PCI). BACKGROUND: Angiographic guidance for PCI has substantial limitations. The superior spatial resolution of OCT could translate into meaningful clinical benefits, although limited data exist to date about their effect on clinical endpoints. METHODS: This was a cohort study based on the Pan-London (United Kingdom) PCI registry, which includes 123,764 patients who underwent PCI in National Health Service hospitals in London between 2005 and 2015. Patients undergoing primary PCI or pressure wire use were excluded leaving 87,166 patients in the study. The primary endpoint was all-cause mortality at a median of 4.8 years. RESULTS: OCT was used in 1,149 (1.3%) patients, intravascular ultrasound (IVUS) was used in 10,971 (12.6%) patients, and angiography alone in the remaining 75,046 patients. Overall OCT rates increased over time (p < 0.0001), with variation in rates between centers (p = 0.002). The mean stent length was shortest in the angiography-guided group, longer in the IVUS-guided group, and longest in the OCT-guided group. OCT-guided procedures were associated with greater procedural success rates and reduced in-hospital MACE rates. A significant difference in mortality was observed between patients who underwent OCT-guided PCI (7.7%) compared with patients who underwent either IVUS-guided (12.2%) or angiography-guided (15.7%; p < 0.0001) PCI, with differences seen for both elective (p < 0.0001) and acute coronary syndrome subgroups (p = 0.0024). Overall this difference persisted after multivariate Cox analysis (hazard ratio [HR]: 0.48; 95% confidence interval [CI]: 0.26 to 0.81; p = 0.001) and propensity matching (hazard ratio: 0.39; 95% CI: 0.21 to 0.77; p = 0.0008; OCT vs. angiography-alone cohort), with no difference in matched OCT and IVUS cohorts (HR: 0.88; 95% CI: 0.61 to 1.38; p = 0.43). CONCLUSIONS: In this large observational study, OCT-guided PCI was associated with improved procedural outcomes, in-hospital events, and long-term survival compared with standard angiography-guided PCI.

Here we test whether genome size is a predictor of pollen size. If it were, inferences of ancient genome size would be possible using the abundant paleo-palynolgical record. We performed regression analyses across 464 species of pollen width and genome size. We found a significant positive trend. However, regression analysis using phylogentically independent contrasts did not support the correlated evolution of these traits. Instead, a large split between angiosperms and gymnosperms for both pollen width and genome size was revealed. Sister taxa were not more likely to show a positive contrast when compared to deeper nodes. However, significantly more congeneric species had a positive trend than expected by chance. These results may reflect the strong selection pressure for pollen to be small. Also, because pollen grains are not metabolically active when measured, their biology is different than other cells which have been shown to be strongly related to genome size, such as guard cells. Our findings contrast with previously published research. It was our hope that pollen size could be used as a proxy for inferring the genome size of ancient species. However, our results suggest pollen is not a good candidate for such endeavors.

Our understanding of the effects of heat stress on plant photosynthesis has progressed rapidly in recent years through the use of chlorophyll a fluorescence techniques. These methods frequently involve the treatment of leaves for several hours in dark conditions to estimate declines in maximum quantum yield of photsystem II (F V/F M), rarely accounting for the recovery of effective quantum yield (ΔF/F M′) after thermally induced damage occurs. Exposure to high temperature extremes, however, can occur over minutes, rather than hours, and recent studies suggest that light influences damage recovery. Also, the current focus on agriculturally important crops may lead to assumptions about average stress responses and a poor understanding about the variation among species’ thermal tolerance. We present a chlorophyll a fluorescence protocol incorporating subsaturating light to address whether species’ thermal tolerance thresholds (T 50) are related to the ability to recover from short-term heat stress in 41 Australian desert species. We found that damage incurred by 15-min thermal stress events was most strongly negatively correlated with the capacity of species to recover after a stress event of 50 °C in summer. Phylogenetically independent contrast analyses revealed that basal divergences partially explain this relationship. Although T 50 and recovery capacity were positively correlated, the relationship was weaker for species with high T 50 values (>51 °C). Results highlight that, even within a single desert biome, species vary widely in their physiological response to high temperature stress and recovery metrics provide more comprehensive information than damage metrics alone.

Summary The strong positive relationship evident between cell and genome size in both animals and plants forms the basis of using the size of stomatal guard cells as a proxy to track changes in plant genome size through geological time. We report for the first time a taxonomic fine-scale investigation into changes in stomatal guard-cell length and use these data to infer changes in genome size through the evolutionary history of land plants. Our data suggest that many of the earliest land plants had exceptionally large genome sizes and that a predicted overall trend of increasing genome size within individual lineages through geological time is not supported. However, maximum genome size steadily increases from the Mississippian (c. 360 million yr ago (Ma)) to the present. We hypothesise that the functional relationship between stomatal size, genome size and atmospheric CO2 may contribute to the dichotomy reported between preferential extinction of neopolyploids and the prevalence of palaeopolyploidy observed in DNA sequence data of extant vascular plants.

A correlation between genome size and cell volume has been observed across diverse assemblages of eukaryotes. We examined this relationship in diatoms (Bacillariophyceae), a phylum in which cell volume is of critical ecological and biogeochemical importance. In addition to testing whether there is a predictive relationship across extant species, we tested whether evolutionary divergences in genome size were correlated with evolutionary divergences in cell size (using independent contrasts). We estimated total DNA content for 16 diatom species using a flow cytometer and estimated cell volumes using critical dimensions with scaling equations. Our independent contrast analyses indicated a significant correlated evolution between genome size and cell volume. We then explored the evolutionary and ecological implications of this evolutionary relationship. Diatom cell volume is an important component of the global carbon cycle; therefore, understanding the mechanisms that drive diatom genome evolution has both evolutionary and ecological importance.

Results are summarized and illustrated from a long series of experiments on ice growth from the vapor, nearly all in a very small range of conditions: −5°C, slightly below liquid water saturation, with minimal environmental gradients and no imposed ventilation. The temperature was chosen because c-axis ice needles grow in a narrow temperature interval there, which coincides with the temperature at which the Hallett–Mossop ice multiplication process operates most effectively, and one may suspect that this coincidence is likely to be meaningful. The ice growth habit is poorly reproducible in these conditions, dictating many runs with little change. Growth as plates can persist for hours, and two distinct types of needle growth occur, called sheath needles and sharp needles. Both are distinct from thin columns in that they taper to a point, with no discernible basal face. Both deviate slightly from parallel to the c axis. Sharp needles have been reported before, but only as occurring with an applied high DC voltage. New crystal orientations nucleate occasionally at the tips of the sharp needles; this also has been seen before in the presence of strong electric fields. There appears to be an ice multiplication mechanism in these conditions that does not involve riming.

As a response to extreme water shortages in southeast Queensland, Australia, brought about by reduced rainfall and increasing population, the Queensland government decided to explore the potential for cloud seeding to enhance rainfall. The Queensland Cloud Seeding Research Program (QCSRP) was conducted in the southeast Queensland region near Brisbane during the 2008/09 wet seasons. In addition to conducting an initial exploratory, randomized (statistical) cloud seeding study, multiparameter radar measurements and in situ aircraft microphysical data were collected. This comprehensive set of observational platforms was designed to improve the physical understanding of the effects of both ambient aerosols and seeding material on precipitation formation in southeast Queensland clouds. This focus on gaining physical understanding, along with the unique combination of modern observational platforms utilized in the program, set it apart from previous cloud seeding research programs. The overarching goals of the ...

During the Queensland Cloud Seeding Research Program, the “CP2” polarimetric radar parameter differential radar reflectivity Zdr was used to examine the raindrop size evolution in both maritime and continental clouds. The focus of this paper is to examine the natural variability of the drop size distribution. The primary finding is that there are two basic raindrop size evolutions, one associated with continental air masses characterized by relatively high aerosol concentrations and long air trajectories over land and the other associated with maritime air masses with lower aerosol concentrations. The size evolution difference is during the growth stage of the radar echoes. The differential radar reflectivity in the growing continental clouds is dominated by large raindrops, whereas in the maritime clouds differential reflectivity is dominated by small raindrops and drizzle. The drop size evolution in many of the maritime air masses was very similar to those observed in the maritime air of the Caribbean Sea observed with the NCAR S-band polarimetric radar (S-Pol) during the Rain in Cumulus over the Ocean (RICO) experiment. Because the tops of the Queensland continental clouds ascended almost 2 times as fast as the maritime ones in their growth stage, both dynamical and aerosol factors may be important for the systematic difference in drop size evolution. Recommendations are advanced for future field programs to understand better the causes for the observed variability in drop size evolution. Also, considering the natural variability in drop size evolution, comments are provided on conducting and evaluating cloud seeding experiments.

Variation in genome size (GS) has been linked to several facets of the plant phenotype. Recently it was shown that GS is significantly correlated with cell size and the duration of the cell cycle. Here we test the hypothesis that GS might also be a predictor of apical root meristem growth rate (RMGR). We studied eight species of eudicots with varying GS using time-lapse microscopic image analysis. A significant negative exponential relationship was observed between GS and RMGR. Our results show significantly decreased RMGR for large genome species. This relationship represents a significant consequence of GS expansion in plants and may partly explain why genome sizes tend to be small in eudicots. Interestingly, parasitic plants, which do not rely on root growth as much, often have large genomes.

We performed a comparative analysis of defensive and nutritional plant traits responsible for differential herbivory in a series of experimental feeding trials with generalist herbivores. We measured three defensive traits (leaf strength, leaf mass per unit area and endophytic fungal infection) and two nutritional traits (foliar nitrogen and water) for 26 native and eight non-native plant species from coastal California shrublands. Our feeding trials involved three species of generalist herbivore (beet armyworm, cabbage looper and the garden snail) in two types of laboratory feeding trial (single plant species and preference tests). All traits were significantly related to the amount of leaf area consumed, with foliar nitrogen followed by leaf strength explaining most of the variation in herbivore damage. Defensive and nutritional traits were tightly correlated with one another. These correlations were still apparent after incorporation of the phylogenetic relationships of species using independent contrasts, suggesting that there has been repeated selection for certain trait combinations. Non-native species had lower defensive traits and greater nutrient content and therefore experienced greater herbivory damage than natives. Poorly defended, nutrient-rich species (like most of the non-natives in our study) may be better suited for rapid growth and nutrient acquisition, thus reducing the cost of replenishing leaf material lost to herbivores. © 2010 The Linnean Society of London, Botanical Journal of the Linnean Society, 2010, 163, 1–13.

The aim of this study is to investigate the significance of nonpolar functional groups in the surface orientation-dependent adsorption of natural and synthetic antifreeze proteins (AFPs) on ice. We investigate using nanoliter osmometry, ice etching, and circular dichroism spectroscopy a series of nine designed α-helical peptides to probe the nature of their selectivity between the bipyramidal {201} and the secondary prismatic {210} faces of ice, identified earlier as the preferred faces for adsorption of winter flounder (WF) and shorthorn sculpin type I AFPs, respectively. Complementarities between the AFP’s surface features, comprised chiefly of nonpolar groups, and the well-defined sites on ice surface, allow close contact. Because our experimental studies demonstrate that the function of AFPs relies on participation of both polar and nonpolar residues in the ice surface adsorption, we carry out computational modeling to explore both kinds of contacts at the ice surface. On the basis of results of semi...

Across eukaryotes phenotypic correlations with genome size are thought to scale from genome size effects on cell size. However, for plants the genome/cell size link has only been thoroughly documented within ploidy series and small subsets of herbaceous species. Here, the first large-scale comparative analysis is made of the relationship between genome size and cell size across 101 species of angiosperms of varying growth forms. Guard cell length and epidermal cell area were used as two metrics of cell size and, in addition, stomatal density was measured. There was a significant positive relationship between genome size and both guard cell length and epidermal cell area and a negative relationship with stomatal density. Independent contrast analyses revealed that these traits are undergoing correlated evolution with genome size. However, the relationship was growth form dependent (nonsignificant results within trees/shrubs), although trees had the smallest genome/cell sizes and the highest stomatal density. These results confirm the generality of the genome size/cell size relationship. The results also suggest that changes in genome size, with concomitant influences on stomatal size and density, may influence physiology, and perhaps play an important genetic role in determining the ecological and life-history strategy of a species.

The 24 June 2006 Boulder hailstorm produced very heavy precipitation including disklike hailstones that grew with low density. These disklike hailstones, 4–5 cm in diameter, are unusual, and some of them appear to have accumulated graupel while aloft. A large amount of very fine-grained slush was left on the ground along with the hail. The hail and the great amount of slush suggest that most of the hydrometeor growth in the cloud was by low- or very-low-density riming. Consistent with that, the radar data suggest that the storm updraft had substantially depleted liquid water content. There is evidence that low-density hydrometeor growth within storms may be considerably more frequent than is commonly suspected.

An unusual, isolated hailstorm descended on Boulder, Colorado, on the evening of 24 June 2006. Starting with scattered large, flattened, disk-shaped hailstones and ending with a deluge of slushy hail that was over 4 cm deep on the ground, the storm lasted no more than 20 min and did surprisingly little damage except to vegetation. Part I of this two-part paper examines the meteorological conditions preceding the storm and the signatures it exhibited on Weather Surveillance Radar-1988 Doppler (WSR-88D) displays. There was no obvious upper-tropospheric forcing for this storm, vertical shear of the low-level wind was minimal, the boundary layer air feeding the storm was not very moist (maximum dewpoint 8.5°C), and convective available potential energy calculated from a modified air parcel was at most 1550 J kg−1. Despite these handicaps, the hail-producing storm had low-level reflectivity exceeding 70 dBZ, produced copious low-density hail, exhibited strong rotation, and generated three extensive bounded weak-echo regions (BWERs) in succession. The earliest of these filled with high reflectivities as the second one to the south poked up through precipitation-filled air. This has implications for low-density hail growth, as discussed in Part II.

Early radar echo development in trade wind cumulus clouds is studied using the equivalent reflectivity factor Ze combined with the differential reflectivity Zdr. The clouds studied are among the largest of trade wind cumulus, developing significantly positive values of Zdr and attaining at least about a 30-dBZ equivalent reflectivity factor. The measures used for analysis are values calculated for entire constant–elevation angle sweeps through the clouds and entire volume scans—not maximum single-pulse-volume values. The radar echo evolution follows fairly closely the Marshall–Palmer distribution with scatter toward higher values of Zdr especially in the earliest stages of echo intensification, where some of the scatter in the whole-sweep values is caused by size sorting. The data provide no evidence for an important role of ultragiant aerosols (UGA) in initiating coalescence. They are in strong contrast with similar data from a cloud over northern Alabama that do suggest a major role for UGA in producing several-mm-diameter raindrops that dominate its weak, early radar echo.

A correlation between genome size and cell volume has been observed across diverse assemblages of eukaryotes. We examined this relationship in diatoms (Bacillariophyceae), a phylum in which cell volume is of critical ecological and biogeochemical importance. In addition to testing whether there is a predictive relationship across extant species, we tested whether evolutionary divergences in genome size were correlated with evolutionary divergences in cell size (using independent contrasts). We estimated total DNA content for 16 diatom species using a flow cytometer and estimated cell volumes using critical dimensions with scaling equations. Our independent contrast analyses indicated a significant correlated evolution between genome size and cell volume. We then explored the evolutionary and ecological implications of this evolutionary relationship. Diatom cell volume is an important component of the global carbon cycle; therefore, understanding the mechanisms that drive diatom genome evolution has both evolutionary and ecological importance.

A tillage and straw management study was started near Delta Junction Alaska (64°49′N, 147°52′W) USA in 1983 to determine the impact of tillage, straw management, and nitrogen fertilizer application...

†Background and Aims It has been proposed that having too much DNA may carry physiological consequences for plants. The strong correlation between DNA content, cell size and cell division rate could lead to predictable morphological variation in plants, including a negative relationship with leaf mass per unit area (LMA). In addition, the possible increased demand for resources in species with high DNA content may have downstream effects on maximal metabolic efficiency, including decreased metabolic rates. †Methods Tests were made for genome size-dependent variation in LMA and metabolic rates (mass-based photosynthetic rate and dark respiration rate) using our own measurements and data from a plant functional trait database (Glopnet). These associations were tested using two metrics of genome size: bulk DNA amount (2C DNA) and monoploid genome size (1Cx DNA). The data were analysed using an evolutionary framework that included a regression analysis and independent contrasts using a phylogenetic tree with estimates of molecular diversification times. A contribution index for the LMA data set was also calculated to determine which divergences have the greatest influence on the relationship between genome size and LMA. †Key Results and Conclusions A significant negative association was found between bulk DNA amount and LMA in angiosperms. This was primarily a result of influential divergences that may represent early shifts in growth form. However, divergences in bulk DNA amount were positively associated with divergences in LMA, suggesting that the relationship may be indirect and mediated through other traits directly related to genome size. There was a significant negative association between genome size and metabolic rates that was driven by a basal divergence between angiosperms and gymnosperms; no significant independent contrast results were found. Therefore, it is concluded that genome size-dependent constraints acting on metabolic efficiency may not exist within seed plants.

Little is known about the long-term effects of tillage and crop residue management on soil quality and organic matter conservation in subarctic regions. Therefore, we quantified wet aggregate stability, bulk density, pH, total organic C and N, inorganic N, microbial biomass C and N, microbial biomass C:N ratio, microbial quotient, and potential C and N mineralization for a tillage/crop residue management study in central Alaska. Soil from no-till (NT), disked once each spring (DO), and disked twice (DT, spring and fall) treatments was sampled to 20 cm depth in spring and fall of the 16th and 17th years of the study. Crop residues were either retained or removed after harvest each year. Reducing tillage intensity had greater impact on most soil properties than removing crop residues with the most notable effects in the top 10 cm. Bulk density was the only indicator that showed significant differences for the 10–20 cm depth, with values of 0.74 Mg m −3 in the surface 10 cm in NT compared to 0.86 in DT and 1.22 Mg m −3 in NT compared to 1.31 in DT for the 10–20 cm depth. Wet aggregate stability ranged from 10% in DT to 20% in NT. Use of NT or DO conserved soil organic matter more than DT. Compared to measurements made in the 3rd and 4th years of the study, the DT treatment lost almost 20% of the soil organic matter. Retaining crop residues on the soil conserved about 650 g m −2 greater C than removing all residues each year. Soil microbial biomass C and mineralizable C were highest in NT, but the microbial C quotient, which averaged only 0.9%, was not affected by tillage or crop residue treatment. Microbial biomass C:N ratio was 11.3 in DT and 14.4 in the NT, indicating an increasing predominance of fungi with decreasing tillage intensity. Barley grain yield, which averaged 1980 kg ha −1 over the entire 17 years of the study, was highest in DO and not significantly different between NT and DT, but weeds were a serious problem in NT. Reduced tillage can improve important soil quality indicators and conserve organic matter, but long-term NT may not be feasible in the subarctic because of weed problems and build up of surface organic matter.

Blooms of toxic algae are increasing in magnitude and frequency around the globe, causing extensive economic and environmental impacts. On the west coast of Florida, blooms of the toxic dinoflagellate Karenia brevis (Davis) have been documented annually for the last 30 years causing respiratory irritation in humans, fish kills, and toxin bioaccumulation in shellfish beds. As a result, methods need to be established to monitor and predict bloom formation and transport to mitigate their harmful effects on the surrounding ecosystems and local communities. In the past, monitoring and mitigation efforts have relied on visual confirmation of water discoloration, fish kills, and laborious cell counts, but recently satellite remote sensing has been used to track harmful algal blooms (HABs) along the Florida coast. Unfortunately satellite ocean color is limited by cloud cover, lack of detection below one optical depth, and revisit frequency, all of which can lead to extended periods without data. To address these shortcomings, an optical phytoplankton discriminator (OPD) was developed to detect K. brevis cells in mixed phytoplankton assemblages. The OPD was integrated into autonomous underwater vehicle (AUV) platforms to gather spatially and temporally relevant data that can be used in collaboration with satellite imagery to provide a 3D picture of bloom dynamics over time. In January 2005, a Remote Environmental Monitoring UnitS (REMUS) AUV with an OPD payload was deployed on the west coast of Florida to retrieve a similarity index (SI), which indicates when K. brevis dominates the phytoplankton community. SI was used to monitor a K. brevis bloom in relation to temperature, salinity, chlorophyll, and ocean currents. Current speed, SI, temperature, salinity, and chlorophyll a from the AUV were used to quantify a 1 km displacement of the K. brevis bloom front that was observed over the deployment period. The ability to monitor short term bloom movement will improve monitoring and predictive efforts that are used to provide warnings for local tourism and fishing industries. In addition, understanding the fine scale environmental conditions associated with bloom formation will increase our ability to predict the location and timing of K. brevis bloom formation. This study demonstrates the use of one autonomous platform and provides evidence that a nested array of AUVs and moorings equipped with new sensors, combined with remote sensing, can provide an early warning and monitoring system to reduce the impact of HABs.

Over the last decade, the introduction of microarray technology has had a profound impact on gene expression research. The publication of studies with dissimilar or altogether contradictory results, obtained using different microarray platforms to analyze identical RNA samples, has raised concerns about the reliability of this technology. The MicroArray Quality Control (MAQC) project was initiated to address these concerns, as well as other performance and data analysis issues. Expression data on four titration pools from two distinct reference RNA samples were generated at multiple test sites using a variety of microarray-based and alternative technology platforms. Here we describe the experimental design and probe mapping efforts behind the MAQC project. We show intraplatform consistency across test sites as well as a high level of interplatform concordance in terms of genes identified as differentially expressed. This study provides a resource that represents an important first step toward establishing a framework for the use of microarrays in clinical and regulatory settings.

Examination of the early radar echo histories of several vigorous, cumulus clouds in northeast Colorado and northwest Kansas, with sensitive, dual-polarization radar, reveals the formation of millimeter-sized water drops at about the same time that the conventional, first precipitation echo (from ice) forms aloft. The early, positive ZDR values appear in the vicinity of the 0°C level (the radar data do not specify height accurately) and soon extend both above and below it. Positive ZDR is found within and to the upwind side of the updraft, separate from the conventional first precipitation echoes, which appear first at higher altitude, generally downwind of the updraft core, and have no significantly positive ZDR. Big, liquid drops were not expected this early in the formation of continental cumulus. The early presence of supercooled water drops larger than cloud droplets may be a significant factor in the glaciation of these clouds. The kind of early radar coverage illustrated here would be a priceless adjunct to aircraft studies of precipitation formation in cumulus. Microphysical data from aircraft must be interpreted with numerical models in order to deduce (or verify) the processes, and such models require the kind of early data illustrated here, both for initialization and verification.

We studied the physiological basis of local adaptation to drought in Boechera holboellii, a perennial relative of Arabidopsis thaliana, and used cDNA-AFLPs to identify candidate genes showing differential expression in these populations. We compared two populations of B. holboellii from contrasting water environments in a reciprocal transplant experiment, as well as in a laboratory dry-down experiment. We continuously measured the water content of soils using time domain reflectometery (TDR). We compared populations for their water use efficiency (WUE), root/shoot ratios (R:S) and leaf mass per unit area (LMA) in the field and in the laboratory, and identified candidate genes that (i) responded plastically to water stress and (ii) were differentially expressed between the two populations. Genotypes from the drier site had higher WUE, which was attributable to a large reduction in transpirational water loss. The xeric-adapted population also had increased investment in root biomass and greater leaf mass per unit area. Reciprocal transplants in the field had significantly greater survival in their native habitat. In total, 450 cDNA-AFLP fragments showed significant changes between drought and control treatments. Furthermore, some genes showed genotype (population)-specific patterns of up- or down-regulation in response to drought. Three hundred cDNA-AFLP bands were sequenced leading to the identification of cDNAs coding for proteins involved in signal transduction, transcriptional regulation, redox regulation, oxidative stress and pathways involved in stress adaptation. Some of these proteins could contribute a physiological advantage under drought, making them potential targets for natural selection.

Growth and inhibition of single crystals of ethylene oxide clathrate hydrate provide experimental accessibility to similar phenomena in methane clathrate hydrates. Ethylene oxide hydrate single crystals were grown in a simple experimental setup. The crystals grew from the subcooled melt as rhombic dodecahedra, macroscopically exhibiting {110} as the slowest growing faces. The addition of minute amounts of poly(N-vinylcaprolactam) (PVCap) or a random terpolymer of PVCap, poly(N-vinylpyrrolidone), and dimethylaminoethylmethacrylate (VC-713) caused small-scale branching of crystals, but with a seemingly uniform crystal orientation. A few tenths of a weight percent of these additives caused complete crystal growth inhibition at several degrees of subcooling.

The Aurora, Nebraska, hailstorm of 22 June 2003 produced some exceptionally large hailstones, and was widely publicized. Nineteen hailstones obtained from local people have been sectioned and photographed and eight are illustrated here, recording their interior layering and external appearance. They exhibit great variability, with features that are common to large hail as well as one unusual growth form: a roughly prolate external shape indicating an approximately constant falling orientation during final growth, forming large, icicle-like projections at one end. Much of the growth is wet but not appreciably spongy, as appears to be common for large hail. While a hailstone from this storm has been called the largest recorded in the United States on the basis of its longest dimension, we suggest that the most meaningful measure of hailstone size is weight. Weight is unambiguous and easily measured, and is an excellent indicator of volume for large hail. People generally think of hail as spherical ...