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1. Anchor Clustering for million-scale immune repertoire sequencing data.

Author

Chang, Haiyang, Ashlock, Daniel, Graether, Steffen, and Keller, Stefan

Subjects
Clonal relationship, Immune repertoire, Lymphocyte antigen receptor, Unsupervised clustering, Algorithms, Cluster Analysis, Receptors, Antigen
Abstract

BACKGROUND: The clustering of immune repertoire data is challenging due to the computational cost associated with a very large number of pairwise sequence comparisons. To overcome this limitation, we developed Anchor Clustering, an unsupervised clustering method designed to identify similar sequences from millions of antigen receptor gene sequences. First, a Point Packing algorithm is used to identify a set of maximally spaced anchor sequences. Then, the genetic distance of the remaining sequences to all anchor sequences is calculated and transformed into distance vectors. Finally, distance vectors are clustered using unsupervised clustering. This process is repeated iteratively until the resulting clusters are small enough so that pairwise distance comparisons can be performed. RESULTS: Our results demonstrate that Anchor Clustering is faster than existing pairwise comparison clustering methods while providing similar clustering quality. With its flexible, memory-saving strategy, Anchor Clustering is capable of clustering millions of antigen receptor gene sequences in just a few minutes. CONCLUSIONS: This method enables the meta-analysis of immune-repertoire data from different studies and could contribute to a more comprehensive understanding of the immune repertoire data space.

Published
2024

2. Canine peripheral blood TCRαβ T cell atlas: Identification of diverse subsets including CD8A+ MAIT-like cells by combined single-cell transcriptome and V(D)J repertoire analysis

Author

Eschke, Maria, Moore, Peter F, Chang, Haiyang, Alber, Gottfried, and Keller, Stefan M

Subjects
Biomedical and Clinical Sciences, Immunology, Genetics, Aetiology, 2.1 Biological and endogenous factors, Inflammatory and immune system, Dogs, Animals, Humans, Receptors, Antigen, T-Cell, alpha-beta, Transcriptome, Mucosal-Associated Invariant T Cells, T-Lymphocytes, Regulatory, Cell Differentiation, dog, canine T cells, TCR alpha beta, single-cell RNA-sequencing, immune repertoire, peripheral blood, MAIT-like cells, innate-like T cells, TCRαβ, Medical Microbiology, Biochemistry and cell biology
Abstract

The dog is valued as a companion animal and increasingly recognized as a model for human disorders. Given the importance of T cells in health and disease, comprehensive knowledge of canine T cells can contribute to our understanding of pathogenesis mechanisms and inform the development of new treatment strategies. However, the diversity of canine T cells is still poorly understood mainly due to the lack of species-reactive antibodies for use in flow cytometry. The aim of this study was to generate a detailed atlas of peripheral blood TCRαβ+ T cells of healthy dogs using single-cell RNA-sequencing (scRNAseq) combined with immune repertoire sequencing. A total of 22 TCRαβ+ T cell clusters were identified, which were classified into three major groups: CD4-dominant (11 clusters), CD8A-dominant (8 clusters), and CD4/CD8A-mixed (3 clusters). Based on differential gene expression, distinct differentiation states (naïve, effector, memory, exhausted) and lineages (e.g. CD4 T helper and regulatory T cells) could be distinguished. Importantly, several T cell populations were identified, which have not been described in dogs before. Of particular note, our data provide first evidence for the existence of canine mucosa-associated invariant T cell (MAIT)-like cells, representing one of three newly identified FCER1G+ innate-like CD8A+ T cell populations in the peripheral blood of healthy dogs. In conclusion, using scRNAseq combined with immune repertoire sequencing we were able to resolve canine TCRαβ+ T cell populations at unprecedented resolution. The peripheral blood TCRαβ+ T cell atlas of healthy dogs generated here represents an important reference data set for future studies and is of relevance for identifying new targets for T cell-specific therapies.

Published
2023

5. Use of immune repertoire sequencing to resolve discordant microscopic and immunochemical findings in a case of T cell-rich large B cell lymphoma in a young dog

Author

Lee, Gary Kwok Cheong, Bienzle, Dorothee, Keller, Stefan Matthias, Hwang, Mei-Hua, Darzentas, Nikos, Chang, Haiyang, Rätsep, Emily, Egan, Rebecca, and Beeler-Marfisi, Janet

Subjects
Cancer, Digestive Diseases, Liver Disease, Lymphoma, Rare Diseases, Hematology, 2.1 Biological and endogenous factors, Aetiology, Inflammatory and immune system, Animals, Antigens, CD, Ascites, Dog Diseases, Dogs, Euthanasia, Animal, Flow Cytometry, Immunohistochemistry, Immunophenotyping, Liver Neoplasms, Lymphocyte Subsets, Lymphoma, Large B-Cell, Diffuse, Male, T-Lymphocytes, Canine, Clonality, Flow cytometry, Liver, Neoplasia, Next-generation sequencing, PCR for antigen receptor gene rearrangements, TCRBL, Immunocytochemistry, Biochemistry and Cell Biology, Microbiology, Veterinary Sciences
Abstract

BackgroundLymphocytic neoplasms with frequent reactive lymphocytes are uncommonly reported in dogs, and can pose a diagnostic challenge. Different diagnostic modalities such as cytology, flow cytometry, histopathology, immunohistochemistry, and clonality testing, are sometimes required for a diagnosis. This report illustrates the value of using a multi-modal diagnostic approach to decipher a complex lymphocytic tumor, and introduces immune repertoire sequencing as a diagnostic adjunct.Case presentationA 10-month-old Great Dane was referred for marked ascites. Cytologic analysis of abdominal fluid and hepatic aspirates revealed a mixed lymphocyte population including numerous large lymphocytes, yielding a diagnosis of lymphoma. Flow cytometrically, abdominal fluid lymphocytes were highly positive for CD4, CD5, CD18, CD45, and MHC II, consistent with T cell lymphoma. Due to a rapidly deteriorating clinical condition, the dog was euthanized. Post mortem histologic evaluation showed effacement of the liver by aggregates of B cells surrounded by T cells, suggestive of hepatic T cell-rich large B cell lymphoma. Immune repertoire sequencing confirmed the presence of clonal B cells in the liver but not the abdominal fluid, whereas reactive T cells with shared, polyclonal immune repertoires were found in both locations.ConclusionsT cell-rich large B cell lymphoma is a rare neoplasm in dogs that may be challenging to diagnose and classify due to mixed lymphocyte populations. In this case, the results of histopathology, immunohistochemistry and immune repertoire sequencing were most consistent with a hepatic B cell neoplasm and reactive T cells exfoliating into the abdominal fluid. Immune repertoire sequencing was helpful in delineating neoplastic from reactive lymphocytes and characterizing repertoire overlap in both compartments. The potential pitfalls of equating atypical cytomorphology and monotypic marker expression in neoplasia are highlighted.

Published
2021

13. Preparation and Properties of Low-Dielectric Polyimide Films Containing Tert-Butyl.

Author

Li, Xin, Zheng, Rongrong, Wang, Cheng, Chang, Haiyang, Chen, Shuwu, Wang, Liyan, Cui, Xue, Liu, Yutao, Li, Junhao, Yu, Guangning, and Shi, Ji

Subjects
POLYIMIDE films, MOLECULAR volume, PERMITTIVITY, BENZOPHENONES, POLYIMIDES, TENSILE strength, MICROELECTRONICS
Abstract

The design of high-performance polyimide (PI) films and understanding the relationship of the structure–dielectric property are of great significance in the field of the microelectronics industry, but are challenging. Herein, we describe the first work to construct a series of novel tert-butyl PI films (denoted as PI-1, PI-2, PI-3, and PI-4) based on a low-temperature polymerization strategy, which employed tetracarboxylic dianhydride (pyromellitic anhydride, 3,3′,4,4′-biphenyl tetracarboxylic anhydride, 4,4′-diphenyl ether dianhydride, and 3,3′,4,4′-benzophenone tetracarboxylic anhydride) and 4,4′-diamino-3,5-ditert butyl biphenyl ether as monomers. The results indicate that introducing tert-butyl branches in the main chain of PIs can enhance the free volume of the molecular chain and reduce the interaction between molecular chains of PI, resulting in a low dielectric constant. Particularly, the optimized PI-4 exhibits an excellent comprehensive performance with a high (5) wt% loss temperature (454 °C), tensile strength (117.40 MPa), and maximum hydrophobic angle (80.16°), and a low dielectric constant (2.90), which outperforms most of the results reported to date. [ABSTRACT FROM AUTHOR]

Published
2024
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17. Nonvolatile Ternary Memory Devices Based on MoO3Nanoparticles Embedded in Conjugated Copolymer with Hierarchy Charge Traps

Author

Yu, Haifeng, Zhou, Yijia, Du, Yanting, Li, Jiayu, Zhao, Songhao, Wang, Shuhong, and Chang, Haiyang

Abstract

A D–A conjugated polymer was used in fabrication of memory devices. However, devices using polymers as active layers alone do not lead to satisfactory results. In this paper, we have synthesized a donor–acceptor conjugated polymer by the Suzuki coupling reaction. The memory devices prepared by the polymer exhibit a ternary memory behavior. MoO3nanoparticles have a large number of oxygen vacancies on their surface that can act as charge traps. MoO3nanoparticles (NPs) are mixed with polymers to prepare memory devices for improved memory performance. The relationship between the mass percentage of MoO3NPs and the storage performance and mechanism of the storage device is studied. The first and second threshold voltages of the memory device are −0.5 and −0.75 V, respectively. A current ratio of 1:102.14:104.75for the “0″, “1”, and “2” resistance states of the device indicates a low probability of read/write errors. This work shows that polymer-embedded nanoparticle materials have practical application value in the field of next-generation nonvolatile memories.

Published
2024
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18. Chimney Technique and Single-Branched Stent Graft for the Left Subclavian Artery Preservation During Zone 2 Thoracic Endovascular Aortic Repair for Type B Acute Aortic Syndromes.

Author

Chang, Haiyang, Jin, Die, Wang, Yongzheng, Liu, Bin, Wang, Wujie, and Li, Yuliang

Abstract

Purpose: The purpose of this study was to evaluate the efficacy and safety of zone 2 thoracic endovascular aortic repair assisted by the chimney technique or single-branched stent graft for the preservation of the left subclavian artery, and summarize our single-center experience with the techniques. Materials and methods: From February 2017 to June 2020, 137 patients who underwent left subclavian artery revascularization during zone 2 thoracic endovascular aortic repair were enrolled. Patients had acute type B aortic dissection and penetrating aortic ulcer associated with intramural hematoma. The chimney technique was performed in 68 patients (group A), and single-branched stent graft was deployed in 69 patients (group B). All procedures were performed during the acute phase. Primary technical success, immediate postoperative endoleak, neurologic complications (stroke or spinal cord ischemia), 30-day mortality, 1-year technical success, all-cause mortality, patency of the left subclavian artery, and reintervention were analyzed. Comparing the occurrence of the Bird-Beak Configuration, defined as a gap between the aortic wall and the sent graft with stent protrusion into the aortic lumen more than 5 mm, was also performed. Results: Primary technique success was achieved in 66 and 67 patients in groups A and B, respectively. The incidence of immediate postoperative endoleak, neurologic complications (stroke or spinal cord ischemia), and 30-day mortality were 5.9%, 1.5%, and 4.4% in group A, and 2.9%, 2.9%, and 2.9% in group B, respectively. During follow-up, the 1-year technical success rate was similar in both groups. All-cause mortality was similar in both groups (3.1% in group A and 4.5% in group B). The patency of the left subclavian artery was not significantly different between the 2 groups with 2 and 3 occlusions in groups A and B, respectively. The rate of reintervention was higher in group B (3.1% vs 1.6%, p=0.536), with a non-significant difference. Bird-Beak Configuration was more prominent in group B with the incidence of 59.42%. Conclusions: Acting as minimally invasive alternatives, both techniques are feasible for left subclavian artery preservation during zone 2 thoracic endovascular aortic repair for type B acute aortic syndromes with encouraging mid-term outcomes. Long-term follow-up is required to confirm these findings. [ABSTRACT FROM AUTHOR]

Published
2023
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19. A highly efficient room-temperature NO2 gas sensor based on three-dimensional core–shell structured CoS2 bridged Co3O4@MoS2.

Author

Chang, Haiyang, Fan, Jiahui, Yang, Kejian, Wang, Cheng, Zhang, Boxuan, Zhang, Wanying, and Chen, Xudong

Subjects
*GAS detectors, *TRANSITION metal oxides, *METALLIC oxides, *TRANSITION metals, *RAW materials, *SURFACE area
Abstract

In recent years, two-dimensional transition metal dihalides have emerged as a subject of growing research interest in the field of gas sensing. This heightened attention can be attributed to their notable characteristics of high surface area ratios, customizable electronic properties of the layers, and a wide range of catalytic capabilities. These unique features make them promising candidates for gas sensing applications and warrant further investigation and exploration in this area. Practical applications of the original TMD (MoS2) gas sensors are limited by their poor gas sensing performance at room temperature (RT), including less-than-full recovery, long response times, and low response speeds. Addressing these challenges is crucial for improving their real-world usability. In this study, we synthesized three-component heterojunctions (Co3O4–CoS2@MoS2) with a controlled morphology and composition using different mass ratios of raw materials. The Co3O4–CoS2@MoS2-2 gas sensor demonstrated exceptional sensitivity to NO2 gas (Ra/Rg = 39.6 in 100 ppm) at room temperature, achieving an ultra-fast response time of merely 3.4 seconds in ambient air. This sensing behavior first benefits from Co3O4's high specific surface area and abundant oxygen vacancy concentration. The second is the synergistic effect of the heterogeneous structure between MoS2 and Co3O4. And finally, the electron holding capacity of the S atom in CoS2. The synergistic effect of the three factors promotes the gas-sensing performance of the sensor. The results we obtained show that this approach is viable to improve the sensing performance of metal oxides under RT conditions and can also be scaled up to include other 2D transition metal dihalide-based materials. [ABSTRACT FROM AUTHOR]

Published
2023
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20. A highly efficient room-temperature NO2 gas sensor based on three-dimensional core–shell structured CoS2 bridged Co3O4@MoS2.

Author

Chang, Haiyang, Fan, Jiahui, Yang, Kejian, Wang, Cheng, Zhang, Boxuan, Zhang, Wanying, and Chen, Xudong

Subjects
GAS detectors, TRANSITION metal oxides, METALLIC oxides, TRANSITION metals, RAW materials, SURFACE area
Abstract

In recent years, two-dimensional transition metal dihalides have emerged as a subject of growing research interest in the field of gas sensing. This heightened attention can be attributed to their notable characteristics of high surface area ratios, customizable electronic properties of the layers, and a wide range of catalytic capabilities. These unique features make them promising candidates for gas sensing applications and warrant further investigation and exploration in this area. Practical applications of the original TMD (MoS2) gas sensors are limited by their poor gas sensing performance at room temperature (RT), including less-than-full recovery, long response times, and low response speeds. Addressing these challenges is crucial for improving their real-world usability. In this study, we synthesized three-component heterojunctions (Co3O4–CoS2@MoS2) with a controlled morphology and composition using different mass ratios of raw materials. The Co3O4–CoS2@MoS2-2 gas sensor demonstrated exceptional sensitivity to NO2 gas (Ra/Rg = 39.6 in 100 ppm) at room temperature, achieving an ultra-fast response time of merely 3.4 seconds in ambient air. This sensing behavior first benefits from Co3O4's high specific surface area and abundant oxygen vacancy concentration. The second is the synergistic effect of the heterogeneous structure between MoS2 and Co3O4. And finally, the electron holding capacity of the S atom in CoS2. The synergistic effect of the three factors promotes the gas-sensing performance of the sensor. The results we obtained show that this approach is viable to improve the sensing performance of metal oxides under RT conditions and can also be scaled up to include other 2D transition metal dihalide-based materials. [ABSTRACT FROM AUTHOR]

Published
2023
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23. Endovascular Repair for Acute Type B Aortic Dissection With Unfavorable Proximal Landing Zone

Author

Wujie Wang, Bin Liu, Chang Haiyang, Yuliang Li, and Yongzheng Wang

Subjects
Adult, Male, Pulmonary and Respiratory Medicine, medicine.medical_specialty, Time Factors, Computed Tomography Angiography, medicine.medical_treatment, Aorta, Thoracic, Prosthesis Design, Revascularization, Aortography, Group B, Occlusion, Humans, Medicine, cardiovascular diseases, Stroke, Aged, Retrospective Studies, Aortic dissection, Aortic Aneurysm, Thoracic, business.industry, Endovascular Procedures, Stent, Perioperative, Middle Aged, medicine.disease, Surgery, Aortic Dissection, Treatment Outcome, Landing zone, Acute Disease, cardiovascular system, Female, Stents, Cardiology and Cardiovascular Medicine, business, Follow-Up Studies
Abstract

Background The study aimed to compare the safety and effectiveness between fenestrated technique with or without chimney stent and single-branched stent graft for isolated left subclavian artery revascularization during endovascular repair of acute type B aortic dissection with unfavorable proximal landing zone. Methods From January 2016 to December 2019, 65 acute type B aortic dissection patients with unfavorable proximal landing zone were treated with either the fenestrated technique (n = 34, group A) or single-branched stent graft (n = 31, group B). Type I endoleak, retrograde type A dissection, stroke, left subclavian artery patency, 30-day mortality, and aortic remodeling were systematically recorded and retrospectively analyzed. Results Technical success rates for groups A and group B were 94.12% and 100%, respectively. Left subclavian artery primary patency was achieved for all enrolled patients. The incidence of type I endoleak, retrograde type A dissection, stroke, and 30-day mortality was 5.9%, 5.9%, 2.9%, and 2.9% in group A, respectively, but none were encountered in group B. Left subclavian artery occlusion was observed in 3 patients in group A and 2 patients in group B during a mean follow-up of 16.18 ± 2.08 months and 15.19 ± 2.68 months, respectively. After the procedure, significant aortic remodeling was detected in both groups during follow-up. Conclusions Both techniques are feasible and safe for isolated left subclavian artery revascularization during endovascular repair for acute type B aortic dissection. Apart from the associated perioperative risks of complications and mortality for the fenestrated technique, both procedures contributed to favorable aortic remodeling.

Published
2022

27. Pencil-like Hollow Carbon Nanotubes Embedded CoP-V 4 P 3 Heterostructures as a Bifunctional Catalyst for Electrocatalytic Overall Water Splitting.

Author

Chang, Haiyang, Liang, Zhijian, Lang, Kun, Fan, Jiahui, Ji, Lei, Yang, Kejian, Lu, Shaolin, Ma, Zetong, Wang, Lei, and Wang, Cheng

Subjects
*CARBON nanotubes, *CATALYSTS, *HYDROGEN as fuel, *HETEROSTRUCTURES, *CLEAN energy, *ENERGY shortages
Abstract

Electrocatalytic water splitting is one of the most efficient ways of producing green hydrogen energy. The design of stable, active, and efficient electrocatalysts plays a crucial role in water splitting for achieving efficient energy conversion from electrical to hydrogen energy, aimed at solving the lingering energy crisis. In this work, CNT composites modified with CoP-V4P3 composites (CoVO-10-CNT-450P) were formed by carbonising a pencil-like precursor (Co3V2O8-H2O) and growing carbon nanotubes in situ, followed by in situ phosphorylation on the carbon nanotubes. In the HER electrocatalytic process, an overpotential of only 124 mV was exhibited at a current density of 10 mA cm−2. In addition, as an OER catalyst, a low overpotential of 280 mV was attained at a current density of 10 mA cm−2. Moreover, there was no noticeable change in the performance of the catalyst over a 90 h test in a continuous total water splitting experiment. The unique electronic structure and hollow carbon nanotube structure of CoVO-10-CNT-450P effectively increased the catalytic active sites, while also significantly improving the electrocatalytic activity. This work provides theoretical guidance for the design and synthetic route of high-performance non-precious metal electrocatalysts, and actively promotes the commercial application of electrochemical water splitting. [ABSTRACT FROM AUTHOR]

Published
2023
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30. Insufficient radiofrequency ablation promotes epithelial-mesenchymal transition mediated by interleukin-6/signal transducer and activator of transcription 3/Snail pathway in the H22 cells

Author

Zhou, Tong, Liu, Bin, Wang, Yongzheng, Wang, Wujie, Chang, Haiyang, Li, Dong, Li, Yuliang, and Song, Zhaomin

Subjects
Interleukins, Ablation (Surgery), Stem cells, Health
Abstract

Byline: Tong. Zhou, Bin. Liu, Yongzheng. Wang, Wujie. Wang, Haiyang. Chang, Dong. Li, Yuliang. Li, Zhaomin. Song Context: Radiofrequency ablation (RFA), an established and minimally invasive therapy for hepatocellular carcinoma, [...]

Published
2020

33. Thoracic Endovascular Aortic Repair for Type B Aortic Dissection Associated with Retrograde Type A Intramural Hematoma

Author

Bin Liu, Yuliang Li, Yongzheng Wang, Dong Li, Jia-Cheng Ye, Hertzanu Yancu, Wujie Wang, Wei Wang, and Chang Haiyang

Subjects
Adult, Male, medicine.medical_specialty, Time Factors, Sudden death, 030218 nuclear medicine & medical imaging, Blood Vessel Prosthesis Implantation, 03 medical and health sciences, Aortic aneurysm, Postoperative Complications, 0302 clinical medicine, Hematoma, Aneurysm, medicine.artery, medicine, Humans, Radiology, Nuclear Medicine and imaging, Aortic rupture, Aged, Retrospective Studies, Aortic dissection, Aorta, Aortic Aneurysm, Thoracic, business.industry, Endovascular Procedures, Middle Aged, medicine.disease, Surgery, Aortic Dissection, Treatment Outcome, 030220 oncology & carcinogenesis, Descending aorta, cardiovascular system, Feasibility Studies, Female, Cardiology and Cardiovascular Medicine, business
Abstract

Purpose To evaluate feasibility and efficacy of thoracic endovascular aortic repair (TEVAR) for type B aortic dissection (TBAD) associated with retrograde type A intramural hematoma (IMH). Materials and Methods From April 2013 to January 2017, 15 consecutive patients with TBAD associated with retrograde type A IMH who underwent TEVAR were reviewed retrospectively. There was no cardiac tamponade, aortic regurgitation, involvement of coronary artery, or sign of cerebral ischemia in these patients. Enhanced CT was used in 4 patients to diagnose malperfusion of abdominal visceral arteries or lower extremity artery and underwent emergent TEVAR. For the remaining 11 patients, repeated enhanced CT after initial medical treatment within 24 hours from onset of pain showed expansion of IMH in 8 patients or presence of periaortic hematoma in 3 patients. Delayed TEVAR was scheduled for these cases. Results Successful deployment of the stent graft was achieved in all patients. There were no severe postoperative complications, such as retrograde type A aortic dissection or aortic rupture. Sudden death occurred in 1 patient 3 months after the procedure. Thrombosis of the false lumen, shrinkage of the diameter of the aorta, and complete absorption of the IMH were observed in the remaining patients at a mean follow-up of 19.8 months ± 6.57. Conclusions TEVAR for treatment of TBAD with retrograde type A IMH is feasible and effective. It represents a treatment option for patients with TBAD associated with type A IMH with a proximal entry tear located in the descending aorta.

Published
2020

35. Study on Polyoxomolybdate [Mo8O26]4− Based Crystalline Compound and Its Polypyrrole Nanocomposite as l-Cysteine Colorimetric Biosensor.

Author

Li, Bohong, Chang, Haiyang, Wang, Chao, and Wang, Shuhong

Subjects
*CYSTEINE, *HYBRID materials, *POLYPYRROLE, *MOLYBDATES, *NANOCOMPOSITE materials, *BIOSENSORS
Abstract

A new octamolybdate based organic–inorganic hybrid compound with unusual Ag–Ag chain, [Ag2(bit)2]2[Mo8O26], was successfully synthesized and well characterized. [Ag2(bit)2]2[Mo8O26] as colorimetric sensor materials for l-cysteine (l-Cys) were investigated for the first time. Similar to other type polyoxometalates (POMs), [Ag2(bit)2]2[Mo8O26] exhibits the good peroxidase-like activity attributed to the nature of [Mo8O26]4−, indicating that the polyoxomolybdate based hybrid materials can perform colorimetric sensing to detection biomolecule such as l-Cys. Moreover, to improve the peroxidase-like activity, [Ag2(bit)2]2[Mo8O26]/PPy nanocomposite was also fabricated, and [Ag2(bit)2]2[Mo8O26]/PPy based colorimetric sensing towards l-Cys exhibits the limit of detection of 0.22 μM in the linear range of 1–100 μM, being superior than most reported nanozyme. [ABSTRACT FROM AUTHOR]

Published
2022
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37. Endovascular stenting and coil embolization for management of radiation-induced pseudoaneurysms of the peripheral arteries

Author

Wujie Wang, Yuliang Li, Wei Wang, Bin Liu, Yongzheng Wang, Chang Haiyang, and Zhen-Guo Liu

Subjects
safety, Male, Medicine (General), medicine.medical_specialty, radiation-induced pseudoaneurysm, medicine.medical_treatment, Endovascular therapy, efficacy, Radiation induced, 030204 cardiovascular system & hematology, stent graft, coil embolization, Biochemistry, 03 medical and health sciences, R5-920, 0302 clinical medicine, medicine, Humans, Embolization, cardiovascular diseases, radiotherapy, Coil embolization, Aged, Retrospective Studies, business.industry, Biochemistry (medical), Endovascular Procedures, Cell Biology, General Medicine, Arteries, Middle Aged, Embolization, Therapeutic, Peripheral, Blood Vessel Prosthesis, Radiation therapy, Treatment Outcome, 030220 oncology & carcinogenesis, Female, Stents, Radiology, business, Aneurysm, False, Retrospective Clinical Research Report, rebleeding
Abstract

Objective This study was performed to evaluate the safety and efficacy of endovascular stenting and embolization for the management of radiation-induced peripheral arterial pseudoaneurysms. Methods Twelve consecutive patients with radiation-induced peripheral arterial pseudoaneurysms (diameter of 10–30 mm and mean size of 20.42 mm) were admitted to our hospital from 1 January 2015 to 31 October 2019. The patients’ baseline characteristics, perioperative parameters, device characteristics, and curative effects were systematically recorded and analyzed. Results The 12 patients comprised 3 men and 9 women with a mean age of 62.90 ± 13.97 years. The iliac artery was the most commonly involved artery, followed by the subclavian artery. In all patients, the pseudoaneurysms were detected by enhanced computed tomography. Twelve covered stents were deployed (7 or 8 mm in diameter and 50 mm in length). Additional coil embolization was performed in three patients (Patients 8, 9, and 10). The primary and secondary technical success rate was 91.7% and 100%, respectively. Apart from one death caused by rebleeding, no patients developed rebleeding during follow-up. Conclusion Endovascular stenting and coil embolization are feasible and effective for the management of radiation-induced peripheral arterial pseudoaneurysms in highly selected patients.

Published
2021

38. The role of bile acid subtypes in the diagnosis of cholangiocarcinoma

Author

Wujie Wang, Yongzheng Wang, Zhe Yu, Shilin Tian, Yuliang Li, Chao Chen, Bin Liu, Die Jin, Chang Haiyang, and Wei Wang

Subjects
medicine.drug_class, Population, digestive system, Bile Acids and Salts, Cholangiocarcinoma, 03 medical and health sciences, 0302 clinical medicine, Gene expression, Biomarkers, Tumor, Medicine, Bile, Humans, 030212 general & internal medicine, KEGG, education, neoplasms, Gene, education.field_of_study, Bile acid, Receiver operating characteristic, business.industry, Area under the curve, General Medicine, Bile Ducts, Intrahepatic, Oncology, Bile Duct Neoplasms, ROC Curve, 030220 oncology & carcinogenesis, Cancer research, Biomarker (medicine), business
Abstract

OBJECTIVES To find the potential biomarkers of cholangiocarcinoma, form a biomarker package, evaluate its efficiency, and validate it. METHODS R software was used to analyze the differential expression of mRNAs between cholangiocarcinoma and adjacent nontumorous tissues, obtained from The Cancer Genome Atlas (TCGA), and enrich the KEGG pathway. Metabo-Profile Inc. performed the comprehensive bile acid profiling and quantitation. The training set concluded 20 cholangiocarcinoma and 20 nontumorous volunteers. Receiver operating characteristic (ROC) curve and accompanying area under the curve (AUC) was calculated. The top four bile acids formed a new biomarker package. The validation set included 15 cholangiocarcinoma and 15 nontumorous, and the sensitivity and specificity of the new biomarker package were tested. RESULTS Gene expression of 36 cholangiocarcinoma and nine adjacent nontumorous tissues was obtained in January 2020. Totally 9887 differential genes were eligible (logFC ≥ 1 or ≤ -1, P

Published
2021

40. Insufficient radiofrequency ablation promotes epithelial-mesenchymal transition mediated by interleukin-6/signal transducer and activator of transcription 3/Snail pathway in the H22 cells

Author

Yongzheng Wang, Wujie Wang, Bin Liu, Zhaomin Song, Tong Zhou, Chang Haiyang, Dong Li, and Yuliang Li

Subjects
0301 basic medicine, Male, STAT3 Transcription Factor, Carcinoma, Hepatocellular, Epithelial-Mesenchymal Transition, Cell, Vimentin, 03 medical and health sciences, Mice, 0302 clinical medicine, Cell Movement, Cell Line, Tumor, medicine, Animals, Humans, Radiology, Nuclear Medicine and imaging, Epithelial–mesenchymal transition, STAT3, Mice, Inbred BALB C, Radiofrequency Ablation, biology, Activator (genetics), Chemistry, Interleukin-6, Liver Neoplasms, Interleukin, General Medicine, Disease Models, Animal, 030104 developmental biology, medicine.anatomical_structure, Oncology, Cell culture, 030220 oncology & carcinogenesis, Cancer research, STAT protein, biology.protein, Snail Family Transcription Factors
Abstract

Context: Radiofrequency ablation (RFA), an established and minimally invasive therapy for hepatocellular carcinoma, has become an important treatment strategy. However, tumor aggressiveness remains a common problem. The epithelial–mesenchymal transition (EMT) is thought to play an important role in this process. Design and Aims: Due to limited sample volumes harvested from patients, we established a heat-treated cell line and a mouse model to investigate the mechanisms of incomplete ablation in EMT. Materials and Methods: We heat-treated H22 and HepG2 cells using a water bath to determine a suitable temperature for incomplete RFA. Male BALB/c mice were orthotopically transplanted with H22 cells and then subjected to incomplete ablation. Changes in the EMT biomarkers were detected by real-time polymerase chain reaction, western blotting, and immunofluorescence. Statistical Analysis: The experimental results are expressed as means ± standard deviations. Results: Incomplete RFA promoted EMT, downregulated E-cadherin, upregulated vimentin and Snail, and enhanced the phosphorylation of signal transducer and activator of transcription 3 (STAT3) both in vivo and in vitro. Moreover, interleukin (IL)-6 secretion increased after heat treatment in the H22 cells. AG490, an IL-6 inhibitor, inhibited the occurrence of EMT. Conclusions: Insufficient ablation performed at low temperature successfully induces EMT and promotes tumor aggressiveness, which is mediated by the IL-6/STAT3/Snail pathway in both cell and mouse models.

Published
2020

41. Percutaneous transhepatic cholangiography versus endoscopic retrograde cholangiography for the pathological diagnosis of suspected malignant bile duct strictures

Author

Chang Haiyang, Bin Liu, Wujie Wang, Yongzheng Wang, Dong Li, Wei Wang, and Yuliang Li

Subjects
Male, medicine.medical_specialty, medicine.medical_treatment, Forceps, Hilum (biology), Constriction, Pathologic, Percutaneous transhepatic cholangiography, Diagnostic Accuracy Study, Sensitivity and Specificity, Cholangiocarcinoma, 03 medical and health sciences, 0302 clinical medicine, Cholangiography, malignant bile duct stricture, forceps biopsy, medicine, Humans, Sampling (medicine), 030212 general & internal medicine, Pathological, percutaneous transhepatic cholangiography, brush cytology, Aged, Cholangiopancreatography, Endoscopic Retrograde, medicine.diagnostic_test, Bile duct, business.industry, endoscopic retrograde cholangiography, General Medicine, medicine.anatomical_structure, Bile Ducts, Intrahepatic, Bile Duct Neoplasms, 030220 oncology & carcinogenesis, Endoscopic retrograde cholangiography, Female, Radiology, business, Research Article
Abstract

To compare the diagnostic performance of percutaneous transhepatic cholangiography and endoscopic retrograde cholangiography for the pathological assessment of suspected malignant bile duct stricture, using brush cytology and forceps biopsy. The study group comprised 79 consecutive patients who underwent pathological assessment for suspected malignant biliary stricture, 38 of whom underwent percutaneous transhepatic cholangiography (group A) and the other 41 underwent endoscopic retrograde cholangiography (group B). The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy were calculated. A subset analysis was performed to determine the effect of location and pathological type of the stricture on diagnostic performance, and complications were analyzed. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy were 86.7%, 100%, 100%, 66.7%, and 89.5%, respectively, in group A, and 77.1%, 100%, 100%, 42.9%, and 80.4%, respectively, in group B. For hilar biliary strictures, the sensitivity and accuracy were superior in group A than in group B. Mild complications (transient c and bile leakage) were identified in 7 cases in each group, all resolved spontaneously within 3 to 5 days. Both brush cytology and forceps biopsy performed during percutaneous transhepatic cholangiography and endoscopic retrograde cholangiography provided good diagnostic sensitivity and accuracy. Therefore, both diagnostic approaches can play an important role in planning therapeutic strategy. However, for strictures located at the hilum, pathology sampling via percutaneous transhepatic cholangiography is preferable to endoscopic retrograde cholangiography, as it provides higher sensitivity and accuracy.

Published
2020

42. Long-term outcomes of elective transcatheter dense coil embolization for splenic artery aneurysms: a two-center experience

Author

Chang Haiyang, Yongzheng Wang, Zhe Yu, Wei Wang, Bin Liu, Chao Chen, Wujie Wang, Zhenting Wang, and Yuliang Li

Subjects
Adult, Male, safety, medicine.medical_specialty, Medicine (General), Long term follow up, efficacy, long-term follow-up, 030204 cardiovascular system & hematology, Splenic artery, coil embolization, splenic artery aneurysms, Biochemistry, Young Adult, 03 medical and health sciences, 0302 clinical medicine, R5-920, medicine.artery, Long term outcomes, Humans, Medicine, cardiovascular diseases, Coil embolization, business.industry, Biochemistry (medical), Cell Biology, General Medicine, Middle Aged, Aneurysm, Embolization, Therapeutic, Blood Vessel Prosthesis, Surgery, Treatment Outcome, Transcatheter, 030220 oncology & carcinogenesis, Female, business, Splenic Artery, Follow-Up Studies, Retrospective Clinical Research Report
Abstract

Objective This study was performed to analyze the long-term follow-up safety and efficacy of transcatheter dense coil embolization for splenic artery aneurysms. Methods Thirty-two patients (18 women, 14 men; age range, 23–56 years; mean age, 43.1 ± 13.6 years) who underwent dense coil embolization for treatment of splenic artery aneurysms from August 2010 to January 2018 were retrospectively reviewed. The size and location of the splenic artery aneurysms, the technical and clinical outcomes of the procedure, and the complications related to the procedure were reviewed. Results The technical success rate of embolization was 100% (mean aneurysm size, 29.4 ± 6.9 mm; range, 20–43 mm). Two (6.3%) patients underwent a successful repeat intervention procedure for recurrent aneurysm perfusion during follow-up (mean, 36 months; range, 6–72 months). No aneurysm ruptured during follow-up. Splenic infarction was observed in 8 of 32 (25%) patients. No patients developed major adverse events related to the procedure, such as splenic abscess or pancreatitis. Conclusions Percutaneous elective transcatheter dense coil embolization is safe and effective to prevent aneurysm rupture and overcome aneurysm recanalization during long-term follow-up.

Published
2020

43. Ursodeoxycholic acid combined with percutaneous transhepatic balloon dilation for management of gallstones after elimination of common bile duct stones

Author

Wujie Wang, Bin Liu, Dong Li, Yuliang Li, Changjun Wang, Yongzheng Wang, Wei Wang, and Chang Haiyang

Subjects
Male, Cholagogues and Choleretics, medicine.medical_specialty, Percutaneous, Common bile duct stones, Urology, Gallstones, Diet, High-Fat, Catheterization, Percutaneous transhepatic removal, 03 medical and health sciences, 0302 clinical medicine, Retrospective Study, medicine, Humans, Endoscopy, Digestive System, Aged, Retrospective Studies, Aged, 80 and over, Common Bile Duct, Common bile duct, business.industry, Ursodeoxycholic Acid, Gastroenterology, Gallbladder, General Medicine, Middle Aged, medicine.disease, Combined Modality Therapy, Dilatation, Ursodeoxycholic acid, Choledocholithiasis, Treatment Outcome, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Balloon dilation, Feasibility Studies, Female, 030211 gastroenterology & hepatology, business, Cholangiography, medicine.drug
Abstract

AIM To evaluate the effectiveness and safety of combined ursodeoxycholic acid and percutaneous transhepatic balloon dilation for management of gallstones after expulsion of common bile duct (CBD) stones. METHODS From April 2014 to May 2016, 15 consecutive patients (6 men and 9 women) aged 45-86 (mean, 69.07 ± 9.91) years suffering from CBD stones associated with gallstones were evaluated. Good gallbladder contraction function was confirmed by type B ultrasonography. Dilation of the CBD and cystic duct was detected. Percutaneous transhepatic balloon dilation of the papilla was performed, ursodeoxycholic acid was administered, and all patients had a high-fat diet. All subjects underwent repeated cholangiography, and percutaneous transhepatic removal was carried out in patients with secondary CBD stones originating from the gallbladder. RESULTS All patients underwent percutaneous transhepatic balloon dilation with a primary success rate of 100%. The combined therapy was successful in 86.7% of patients with concomitant CBD stones and gallstones. No remaining stones were detected in the gallbladder. Transient adverse events include abdominal pain (n = 1), abdominal distension (n = 1), and fever (n = 1). Complications were treated successfully via nonsurgical management without long-term complications. No procedure-related mortality occurred. CONCLUSION For patients with concomitant CBD stones and gallstones, after percutaneous transhepatic removal of primary CBD stones, oral ursodeoxycholic acid and a high-fat diet followed by percutaneous transhepatic removal of secondary CBD stones appear to be a feasible and effective option for management of gallstones.

Published
2018

44. Comparison between computed tomography-guided percutaneous microwave ablation and thoracoscopic lobectomy for stage I non-small cell lung cancer

Author

Pi-Kun Cao, Bin Liu, Dong Li, Xiao Li, Yuliang Li, Wujie Wang, Xiaogang Zhao, Yongzheng Wang, Wei Wang, and Chang Haiyang

Subjects
Pulmonary and Respiratory Medicine, medicine.medical_specialty, Stage I Non-Small Cell Lung Cancer, Percutaneous, medicine.diagnostic_test, business.industry, Microwave ablation, Rate control, Computed tomography, General Medicine, medicine.disease, Comorbidity, 030218 nuclear medicine & medical imaging, Surgery, 03 medical and health sciences, 0302 clinical medicine, Oncology, 030220 oncology & carcinogenesis, Overall survival, Medicine, business, Survival analysis
Abstract

Background The study was conducted to investigate the effectiveness and cost of computed tomography (CT)-guided percutaneous microwave ablation (MWA) and thoracoscopic lobectomy for stage I non-small cell lung cancer (NSCLC). Methods We retrospectively analyzed the data of 46 and 85 patients with stage I NSCLC treated with CT-guided percutaneous MWA or thoracoscopic lobectomy, respectively, at our center from July 2013 to June 2015. Overall survival (OS), disease-free survival (DFS), local control rate, hospital stay, and cost were evaluated. Survival curves were constructed using the Kaplan-Meier method and compared using the log-rank test. Results The one and two-year OS rates were 97.82% and 91.30% and 97.65% and 90.59% in the MWA and lobectomy groups, respectively. The one and two-year DFS rates were 95.65% and 76.09% and 95.29% and 75.29%, respectively. No significant differences were observed in log-rank analysis between the groups (P = 0.169). The hospital stays in the MWA and lobectomy groups were 6.62 ± 2.31 and 9.57 ± 3.19 days, respectively. The costs of MWA and lobectomy were US$3274.50 ± US$233.91 and US$4678.87 ± US$155.96, respectively. The differences were all significant (P = 0.003). Conclusion MWA and thoracoscopic lobectomy for stage I NSCLC demonstrate similar one and two-year OS and DFS, with no significant differences between the two groups. MWA involved a shorter hospital stay and lower cost, thus should be considered a better option for patients with severe cardiopulmonary comorbidity and patients unwilling to undergo surgery.

Published
2018

45. The role of bile acid subtypes in the diagnosis of cholangiocarcinoma.

Author

Wang, Wei, Tian, Shi‐Lin, Jin, Die, Liu, Bin, Wang, Wujie, Chang, Haiyang, Chen, Chao, Yu, Zhe, Wang, Yong‐Zheng, and Li, Yu‐Liang

Subjects
BILE acids, CHOLANGIOCARCINOMA, RECEIVER operating characteristic curves
Abstract

Objectives: To find the potential biomarkers of cholangiocarcinoma, form a biomarker package, evaluate its efficiency, and validate it. Methods: R software was used to analyze the differential expression of mRNAs between cholangiocarcinoma and adjacent nontumorous tissues, obtained from The Cancer Genome Atlas (TCGA), and enrich the KEGG pathway. Metabo‐Profile Inc. performed the comprehensive bile acid profiling and quantitation. The training set concluded 20 cholangiocarcinoma and 20 nontumorous volunteers. Receiver operating characteristic (ROC) curve and accompanying area under the curve (AUC) was calculated. The top four bile acids formed a new biomarker package. The validation set included 15 cholangiocarcinoma and 15 nontumorous, and the sensitivity and specificity of the new biomarker package were tested. Results: Gene expression of 36 cholangiocarcinoma and nine adjacent nontumorous tissues was obtained in January 2020. Totally 9887 differential genes were eligible (logFC ≥ 1 or ≤ –1, P < 0.05, and adjust P < 0.01). GO analysis showed that 20 KEGG pathways were enriched, including primary bile acid biosynthesis and bile secretion. Comprehensive bile acid profiling and quantitation showed 15 differential bile acid types, and the ROC‐AUC was between 0.953 and 0.750. HDCA, isoLCA, bCDCA, and DCA were selected to form a biomarker package. The Logit (p = cholangiocarcinoma) = 7.898 – 3.70*(1isoLCA) – 0.444*(bCDCA) + 0.415*(HDCA) + 0.041*DCA. Its ROC‐AUC was 0.944. In the validation set, the sensitivity was 0.933 and the specificity was 0.867. Conclusion: Bile acid types package was efficient to distinguish nontumorous population and cholangiocarcinoma. The difference might be associated to the downregulation of primary bile acid biosynthesis and bile secretion pathway of cholangiocarcinoma. [ABSTRACT FROM AUTHOR]

Published
2022
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48. The Interleukin-6/Signal Transducer and Activator of Transcription 3/Snail Pathway Mediates Epithelial-Mesenchymal Transition in Recurrent Liver Cancer after Radiofrequency Ablation

Author

Yuliang Li, Yongzheng Wang, Wei Wang, Chao Chen, Wujie Wang, Chuanxin Wang, Tong Zhou, Dong Li, Pi-Kun Cao, Chang Haiyang, and Bin Liu

Subjects
biology, Radiofrequency ablation, business.industry, Activator (genetics), Interleukin, Vimentin, medicine.disease, law.invention, law, Hepatocellular carcinoma, medicine, STAT protein, biology.protein, Cancer research, Epithelial–mesenchymal transition, business, STAT3
Abstract

Background: Radiofrequency ablation (RFA), as a relatively mature and minimally invasive therapy for hepatocellular carcinoma (HCC), has become an important treatment strategy. However, tumor recurrence remains common and epithelial-mesenchymal transition (EMT) is thought to play an important role in this process. Based on limited sample volumes harvested from patients, we established a heat-treated cell line and mouse model, in addition to collecting patient samples, to investigate the mechanism of recurrence after RFA due to incomplete ablation. Methods: We heat-treated H22 cells using a water bath to determine a suitable temperature for incomplete RFA. Male BALB/c mice were orthotopically transplanted with H22 cells and then subjected to incomplete ablation. Clinical specimens collected from patients with HCC who had undergone RFA were also collected. Changes in EMT biomarkers were detected by real-time polymerase chain reaction, western blotting, and immunofluorescence. Findings: Incomplete RFA promoted EMT, downregulated E-cadherin, upregulated vimentin and Snail, and enhanced the phosphorylation of signal transducer and activator of transcription 3 (STAT3) both in vivo and in vitro. Moreover, interleukin (IL)-6 secretion increased after heat treatment in H22 cells. AG490, an IL-6 inhibitor, was found to block the occurrence of the EMT. Interpretation: Insufficient ablation performed at low temperatures can successfully induce EMT, which is mediated by the IL-6/STAT3/Snail pathway in cell and mouse models. Funding: Supported by National Natural Science Foundation of China (61671276) and the Natural Science Foundation of Shandong Province (2014ZRE27479, ZR2018PH032, ZR201709250376). Declaration of Interest: All authors declare no conflicts-of-interest related to this article. Ethical Approval: The study design was approved by the appropriate ethics review board.

Published
2018

50. Percutaneous transhepatic papilla balloon dilatation combined with a percutaneous transcystic approach for removing concurrent gallbladder stone and common bile duct stone in a patient with billroth II gastrectomy and acute cholecystitis

Author

Chang Haiyang, Bin Liu, Wei Wang, Dong Li, Yongzheng Wang, Yuliang Li, Zheng Li, and Wujie Wang

Subjects
common bile duct stone, Male, medicine.medical_specialty, Percutaneous, medicine.medical_treatment, Cholecystitis, Acute, Gallstones, Gallbladder Stone, percutaneous transhepatic papilla dilation, 03 medical and health sciences, 0302 clinical medicine, Gastrectomy, medicine, acute cholecystitis, Humans, gallbladder stone, Clinical Case Report, Cholangiopancreatography, Endoscopic Retrograde, Common bile duct, business.industry, Gallbladder, Cholecystolithiasis, General Medicine, Middle Aged, medicine.disease, Dilatation, Major duodenal papilla, medicine.anatomical_structure, Treatment Outcome, 030220 oncology & carcinogenesis, Cholecystitis, 030211 gastroenterology & hepatology, percutaneous transcystic, Radiology, business, Research Article
Abstract

Background: A 61-year-old man presented with upper abdominal pain and jaundice. Abdominal computed tomography imaging revealed stones in the gallbladder and the common bile duct, with a thickening of the gallbladder wall and an obvious increase in the volume of the gallbladder. Initial treatment using endoscopic retrograde cholangiopancreatography failed due to the presence of surgically altered gastrointestinal anatomy. Stones in the gallbladder and common bile duct were subsequently removed concurrently via percutaneous transhepatic papilla balloon dilatation combined with a percutaneous transcystic approach. Liver function recovered rapidly, with total bilirubin and direct bilirubin levels decreasing to normal, with a concomitant improvement in hemoglobin and thrombocyte levels and resolution of the upper abdominal pain and jaundice. Conclusion: Percutaneous transhepatic papilla balloon dilatation, combined with a percutaneous transcystic approach, provided an effective alternative treatment for removing concurrent stones in the common bile duct and gallbladder in a patient with a previous Billroth II gastrectomy and presenting with an acute cholecystitis.

Published
2017

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