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Submitted by Linda Santos (linda_karolayne@hotmail.com) on 2022-11-07T17:25:19Z No. of bitstreams: 5 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Disserta????o de Mestrado .pdf: 972417 bytes, checksum: 942f7e5cf660b0b87acf3050a89301d5 (MD5) CartaEncaminhamentoTCC-TESE-DISSERTA????O.pdf: 129678 bytes, checksum: 00322eec25fd06c1cdafc48c785ee32d (MD5) SEI_UFAM - 1119637 - Ata.pdf: 193988 bytes, checksum: bc3beaaffe278cb6e75be8a8ba47cfb2 (MD5) SEI_23105.039054_2022_14.pdf: 74898 bytes, checksum: 0611d10bdda6bcd44d3c08b66eba9c77 (MD5) Approved for entry into archive by PPGBIOTEC Biotecnologia (ppgbiotecsecretaria@ufam.edu.br) on 2022-11-07T18:04:37Z (GMT) No. of bitstreams: 5 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Disserta????o de Mestrado .pdf: 972417 bytes, checksum: 942f7e5cf660b0b87acf3050a89301d5 (MD5) CartaEncaminhamentoTCC-TESE-DISSERTA????O.pdf: 129678 bytes, checksum: 00322eec25fd06c1cdafc48c785ee32d (MD5) SEI_UFAM - 1119637 - Ata.pdf: 193988 bytes, checksum: bc3beaaffe278cb6e75be8a8ba47cfb2 (MD5) SEI_23105.039054_2022_14.pdf: 74898 bytes, checksum: 0611d10bdda6bcd44d3c08b66eba9c77 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2022-11-07T18:29:37Z (GMT) No. of bitstreams: 5 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Disserta????o de Mestrado .pdf: 972417 bytes, checksum: 942f7e5cf660b0b87acf3050a89301d5 (MD5) CartaEncaminhamentoTCC-TESE-DISSERTA????O.pdf: 129678 bytes, checksum: 00322eec25fd06c1cdafc48c785ee32d (MD5) SEI_UFAM - 1119637 - Ata.pdf: 193988 bytes, checksum: bc3beaaffe278cb6e75be8a8ba47cfb2 (MD5) SEI_23105.039054_2022_14.pdf: 74898 bytes, checksum: 0611d10bdda6bcd44d3c08b66eba9c77 (MD5) Made available in DSpace on 2022-11-07T18:29:37Z (GMT). No. of bitstreams: 5 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Disserta????o de Mestrado .pdf: 972417 bytes, checksum: 942f7e5cf660b0b87acf3050a89301d5 (MD5) CartaEncaminhamentoTCC-TESE-DISSERTA????O.pdf: 129678 bytes, checksum: 00322eec25fd06c1cdafc48c785ee32d (MD5) SEI_UFAM - 1119637 - Ata.pdf: 193988 bytes, checksum: bc3beaaffe278cb6e75be8a8ba47cfb2 (MD5) SEI_23105.039054_2022_14.pdf: 74898 bytes, checksum: 0611d10bdda6bcd44d3c08b66eba9c77 (MD5) Previous issue date: 2022-08-31 The use of medicinal plants in the treatment of diseases is a practice that goes beyond generations, because of this, science has developed phytotherapy, which comprises the use of these plants, for the elaboration or as part of allopathy for the treatment and prevention of diseases, obtained from scientifically proven studies. In this sense, Brazil and the Amazon forest have a wealth of natural resources, whose application in the area of health is present among people. Therefore, studies focused on these actions have been taking up space in research. Couepia edulis Prance is a tree present in the central part of the Amazon that has fruits called agouti, the oil of its almond has potential in the fight against cardiovascular diseases, due to the presence of bioactive compounds such as tocopherols, essential fatty acids, phenolic compounds and unsaturated fatty acids. Such diseases have a high mortality rate worldwide and directly impair blood circulation, affecting the heart and other organs, such as the lungs, liver, kidneys and brain, which are important to ensure the proper functioning of the body. The objective was to test in vitro the almond oil from Couepia edulis Prance and to analyze its bioactive potential to treat cardiovascular diseases. The study aimed, in its methodology, to quantify and verify the nutritional characteristics, physical-chemical and bioactive properties, in addition to the cytotoxicity of agouti nut oil. The results showed that the almond has essential nutrients for humans, enabling its consumption. As for the oil, it has a significant amount of phenolic compounds, placing it as a potential treatment agent for cardiovascular diseases, in addition, it showed low toxicity. Therefore, it can be concluded that, in in vitro analyses, agouti nut oil has the potential to become a herbal medicine when its effects are also verified through in vivo studies. A utiliza????o de plantas medicinais na terap??utica de doen??as ?? uma pr??tica que ultrapassa gera????es, por conta disso, a ci??ncia desenvolveu a fitoterapia, que compreende o uso dessas plantas, para a elabora????o ou como parte na alopatia para o tratamento e preven????o de enfermidades, obtidas a partir de estudos cientificamente comprovados. Nesse sentido, o Brasil e a floresta amaz??nica possuem uma riqueza de recursos naturais, cuja aplica????o na ??rea da sa??de se faz presente entre as pessoas. Sendo assim, os estudos voltados para essas a????es v??m tomando espa??o nas pesquisas. A Couepia edulis Prance ?? uma ??rvore presente na parte central da Amaz??nia que possui frutos chamados de castanha-de cutia, o ??leo de sua am??ndoa tem potencial no combate de doen??as cardiovasculares, devido ?? presen??a de compostos bioativos como tocofer??is, ??cidos graxos essenciais, compostos fen??licos e ??cidos graxos insaturados. Tais doen??as possuem uma alta taxa de mortalidade a n??vel mundial e prejudicam diretamente a circula????o sangu??nea afetando o cora????o, e outros ??rg??os, como: pulm??o, f??gado, rins e c??rebro, que s??o importantes para garantir o funcionamento adequado do corpo. O objetivo foi testar in vitro o ??leo da am??ndoa da Couepia edulis Prance e analisar o seu potencial bioativo para tratar as doen??as cardiovasculares. O estudo visou em sua metodologia, quantificar e verificar as caracter??sticas nutricionais, propriedades f??sico-qu??micas e bioativas, al??m da citotoxidade do ??leo castanha-de cutia. Os resultados evidenciaram que a am??ndoa apresenta nutrientes essenciais para os seres humanos, possibilitando seu consumo. Quanto ao ??leo, este apresenta quantidade significativa de compostos fen??licos e tocofer??is colocando-o como potencial agente tratador de doen??as cardiovasculares, al??m disso, apresentou baixa toxidade. Portanto, pode-se concluir que nas an??lises in vitro, o ??leo da castanha-de-cutia tem potencial para se tornar um fitoter??pico ao tamb??m serem verificados seus efeitos por meio de estudos in vivo.

Submitted by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2019-12-27T15:22:16Z No. of bitstreams: 2 FA??ANHA_FINAL_DA_TESE _(26-11-19 - C??pia (1).pdf: 2722533 bytes, checksum: a0a438a46b086e84c73a1174d2c3e648 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Submitted by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2019-12-27T15:27:17Z No. of bitstreams: 2 Reprodu????o N??o Autorizada 2.pdf: 47716 bytes, checksum: 0353d988c60b584cfc9978721c498a11 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2020-01-03T14:43:26Z (GMT) No. of bitstreams: 2 Reprodu????o N??o Autorizada 2.pdf: 47716 bytes, checksum: 0353d988c60b584cfc9978721c498a11 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Made available in DSpace on 2020-01-03T14:43:26Z (GMT). No. of bitstreams: 2 Reprodu????o N??o Autorizada 2.pdf: 47716 bytes, checksum: 0353d988c60b584cfc9978721c498a11 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2019-11-14 Non-alcoholic fatty liver disease (NAFLD) has been considered the most common form of liver disease in the western world, due to the increased prevalence of obesity in developed and developing countries. Endopleura uchi (Huber) Cuatrec is a native Amazonian tree species with proven herbal properties in several works by researchers from the region and around the world. From its shell is isolated a substance called Bergenin, which has among its properties, antioxidant, hepatoprotective and hypolipidemic activities. The aim of this study was to evaluate the protective effect of bergenin and dry extract of endopleura uchi on the treatment of induced NAFLD in mice and obesity. NAFLD and obesity were induced in male C57BL / 6J mice by Hypercaloryc and Hyperlipid Feed (HF). Sixty male C57BL / 6J mice were divided into 10 groups of six animals each, with the following characteristics: GM1, Negative Control; GM2, Sham Group: Positive Control; GM3, fed HF for eight weeks and then treated with dry Endopleura uchi extract, 200mg/kg/day for seven weeks; GM4, similar to the above, treated with 100mg/kg/day of E. uchi extract; GM5, HF-fed for seven weeks and treated concomitantly with 200mg/kg/day of Endopleura uchi dry extract; GM6, similar to GM5, treated with 100mg/kg/day to verify a potential prophylactic effect at both doses. Groups GM7 and GM8, fed HF for eight weeks and then treated with 50 and 25mg/kg/day for seven weeks with aqueous Bergenin solution respectively; GM9 and GM10, similar to GM5 and GM6 prophylactic groups and treated with aqueous Bergenin solution at doses of 50 and 25 mg/kg/day, respectively. Biochemical examinations of liver function tests and histopathology were performed to prove the anti-obesity effects and treatment of NAFLD of both solutions. Descriptive data analysis was performed and the results are presented in tables, graphs/figures and descriptive measures, such as mean, standard error, among others. To verify the influence of treatments, ANOVA with post hoc test by Student Newman Keuls was used. Having significant influence of the treatments, the Tukey test was used to verify the differences between the means of the groups. In all comparisons, it was considered statistically significant when p

Submitted by Helaine Suassuna (helaine_cavalcante@hotmail.com) on 2019-12-27T17:45:06Z No. of bitstreams: 2 Reprodu????o N??o Autorizada.pdf: 47716 bytes, checksum: 0353d988c60b584cfc9978721c498a11 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2020-01-06T15:43:10Z (GMT) No. of bitstreams: 2 Reprodu????o N??o Autorizada.pdf: 47716 bytes, checksum: 0353d988c60b584cfc9978721c498a11 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Made available in DSpace on 2020-01-06T15:43:10Z (GMT). No. of bitstreams: 2 Reprodu????o N??o Autorizada.pdf: 47716 bytes, checksum: 0353d988c60b584cfc9978721c498a11 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2019-06-28 CNPq - Conselho Nacional de Desenvolvimento Cient??fico e Tecnol??gico Diabetes mellitus is metabolic alteration which involves the metabolism of carbohydrates, resulting in decrease of peripheral tissues response to insulin and increased glycemia, which leads to complications such as nephropathy, neuropathy and retinopathy. In addition, it is associated with modification of the immune response, leading in increased production of inflammatory mediators, triggering chronic inflammation. Natural products are sources of new antidiabetic and anti-inflammatory drugs, thus being fundamental for the development of prototypes that may serve as a basis for drugs development in the future. The aim of this work was synthesize a new derivative from reaction between naringenin and aminoguanidine and to elucidate its anti-diabetic and anti-inflammatory properties. The condensation reaction between aminoguanidine and naringenin produced a derivative characterized as a hydrazine carboximidamide (DCHA) in a post-reaction yield of 81%. DCHA was chemically characterized by mass spectrometry, NMR and IV. The DCHA didn???t exhibited toxicity in murine fibroblasts up to 50 ug / ml. The DCHA demonstrated antioxidant activity upper to naringenin and satisfactory glycation inhibition in vitro. In silico assays. DCHA showed affinity to iNOS, COX and PDE4, thus suggesting that molecule could exhibit anti-inflammatory and hypoglycemic activities. In the antidiabetic activity tests, DCHA decreased the glycemic levels of diabetic mice after 21 days of oral treatment (30mg / kg), thus wielding a hypoglycemic effect. In the anti-inflammatory activity assays, DCHA showed a significant anti-inflammatory response by suppressing the leukocytes migration into the peritoneal cavity in LPS-mice exposed and treated at a dose of 1mg / kg, inhibiting the activation of macrophages in vitro and decreasing edema in a model of zimozan-induced arthritis in mice when treated orally (5 mg / kg). The results obtained demonstrate that it was possible to obtain a new chemical entity from reaction between naringenin and aminoguanidine with antioxidative, hypoglycemic and anti - inflammatory activity which can be further explored in the development of a prototype for treatment of chronic diseases such as diabetes or other processes inflammatory drugs. O diabetes mellitus ?? altera????o metab??lica que envolve o metabolismo dos carboidratos, resultante da diminui????o da resposta dos tecidos perif??ricos ?? insulina e aumento da glicemia, o que leva ao surgimento de complica????es como nefropatia, neuropatia e retinopatia. Al??m disso, est?? associado ?? modifica????o da resposta imune, tendo como consequ??ncia, o aumento da produ????o de mediadores inflamat??rios, desencadeando a inflama????o cr??nica. Os produtos naturais s??o fontes de novos f??rmacos antidiab??ticos e anti-inflamat??rios, assim sendo fundamentais para o desenvolvimento de prot??tipos que poder??o servir como base para o desenvolvimento de medicamentos no futuro. O objetivo deste trabalho foi sintetizar um novo derivado a partir da rea????o entre a naringenina e a aminoguanidina e elucidar suas propriedades antidiab??ticas e antiinflamat??rias. A rea????o de condensa????o entre aminoguanidina e naringenina gerou um derivado caracterizado como uma hidrazina carboximidamina (DCHA), com um rendimento p??s-reacional de 81%. O DCHA foi caracterizado quimicamente por meio de Espectrometria de massas, RMN e IV. O DCHA n??o apresentou toxicidade em fibroblastos murinos at?? a concentra????o de 50 ug/ml. O DCHA apresentou atividade antioxidante superior a naringenina e apresentou inibi????o satisfat??ria da glica????o, demonstrando elevado potencial antiglica????o in vitro. Nos ensaios in silico, o DHCA apresentou afinidade pela iNOS, COX e PDE4, sugerindo assim que a mol??cula poderia apresentar atividades anti-inflamat??ria e hipoglicemiante. Nos ensaios de atividade antidiab??tica, o DCHA diminuiu os n??veis de glic??micos de camundongos diab??ticos ap??s 21 dias de tratamento via oral (30mg/kg), exercendo, portanto, um efeito hipoglicemiante. Nos ensaios de atividade anti-inflamat??ria, o DCHA apresentou significativa resposta anti-inflamat??ria, por suprimir a migra????o de leuc??citos para a cavidade peritoneal em camundongos expostos ao LPS e tratados na dose de 1mg/kg, por inibir a ativa????o de macr??fagos in vitro e por reduzir o edema em modelo de artrite induzida por zimozan em camundongos quando tratados por via oral (5 mg/kg). Os resultados obtidos demonstram que foi poss??vel obter uma nova entidade qu??mica a partir da rea????o da naringerina com a aminoguanidina com atividade antioxidante, hipoglicemiante e anti-inflamat??ria, a qual pode ser futuramente explorada no desenvolvimento de um prot??tipo para tratamento de doen??as cr??nicas como o diabetes ou outros processos inflamat??rios.

Submitted by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2019-05-13T15:50:08Z No. of bitstreams: 2 Reprodu????o N??o Autorizada.pdf: 47716 bytes, checksum: 0353d988c60b584cfc9978721c498a11 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2019-05-13T17:30:47Z (GMT) No. of bitstreams: 2 Reprodu????o N??o Autorizada.pdf: 47716 bytes, checksum: 0353d988c60b584cfc9978721c498a11 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Made available in DSpace on 2019-05-13T17:30:47Z (GMT). No. of bitstreams: 2 Reprodu????o N??o Autorizada.pdf: 47716 bytes, checksum: 0353d988c60b584cfc9978721c498a11 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2019-02-28 92 98221-7372 The productive diversification in the Amazon region is favored by the abundance and availability of products, and biotechnology has stood out as an alternative in the innovative process of quality and sustainable economic development. Based on this view, it is expected that the bioproducts of this biome may favor the treatment of chronic noncommunicable diseases (CNCD). In this context, the objective of this project was to verify the in vitro and in vivo biological and chemical activities of the crude hydroalcoholic extract (CHE) based on camu-camu (Myrciaria dubia (Kunth) McVaugh), a??a?? (E. precatoria Mart.) and guaran?? (Paullinia cupana) in the treatment of dyslipidemias. The enzymatic inhibition assays were performed by indirect fluorimetric method and the tests of cell viability, antioxidants by colorimetric assays and the analyzes of ascorbic acids (AA) and ??-carotene, and of the minerals were by HPLC and spectrometry, respectively. The evaluation of the formulation on the murinometric and biochemical measurements were performed in preclinical trials with rats (n=30) of the Wistar strain (Rattus norvegicus), male and female, divided into five groups (G) randomly (n = 6) : G1 control, G2 induced obesity with experimental diet (hypercaloric and hyperlipidemic), G3 induced obesity with experimental feed intake and supplemented with simvastatin (50 mg/kg/day, and G4 and G5 were induced to obesity with the experimental diet was supplemented with 100 mg/kg/day and 200 mg/kg/day of the formulation, respectively. Biochemical parameters were evaluated in rats plasma by means of appropriate kits. In this study, the formulation showed free radical scavenger activity in the tests of ABTS ?? +, DPPH ?? and FRAP (1,245.66 ?? 1.93 Eq., IC50 = 24.30 ??g / mL and 1,115.25, respectively). The formulation also exhibited cellulite antioxidant activity air in a concentration-dependent manner, in addition to not showing cellular cytotoxic effects, but did not inhibit the activities of digestive enzymes. However, the formulation significantly decreased (p

Submitted by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2019-07-10T19:59:15Z No. of bitstreams: 2 Reprodu????o N??o Autorizada.pdf: 47716 bytes, checksum: 0353d988c60b584cfc9978721c498a11 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2019-07-19T13:55:09Z (GMT) No. of bitstreams: 2 Reprodu????o N??o Autorizada.pdf: 47716 bytes, checksum: 0353d988c60b584cfc9978721c498a11 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Made available in DSpace on 2019-07-19T13:55:09Z (GMT). No. of bitstreams: 2 Reprodu????o N??o Autorizada.pdf: 47716 bytes, checksum: 0353d988c60b584cfc9978721c498a11 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2018-12-17 92 981241860 Copaiba oil from several species of the genus copaifera has been used for many years, commercially, because of its possible modulatory action on inflammatory processes. Phytochemical studies of the species, which elucidate their chemical composition, fractionated in sesquiterpenes and diterpenes, have been performed. Although crude oil is well studied, it is scarce to know the effect of its isolated substances, and there are few studies on the structural modifications of these diterpene acids, seeking to improve their biological activities. In this sense, the present work had as objectives the isolation of the majority diterpenic acids present in the Copaifera multijuga oil: copalic and 3-hydroxy-copalic acids and Copaifera reticulata: hardwickiic acid, with structural modifications in the 2 (two) lowcost and optimized chemistries, as well as in vitro and in vivo assays of the substance with the best anti-inflammatory screening and subsequent in silico studies. After isolation, the substances were semi-synthesized and their structures elucidated by 1H and 13C NMR analysis, and therefore, tested in cell viability assay and TNF-?? inhibition activity check test in vitro. The in vivo model of pleurisy/pleuritis in balb-c mice was performed with the best anti-inflammatory substance (ACD13B-esterification of ibuprofen with 3-hydroxy-copalic acid), as well as expression assay of IKB-?? protein. In addition, the gastrointestinal test was also performed with the same substance. The semi-synthesized derivatives were: ACD4- epoxidation of copalic acid, ACD13B-esterification of ibuprofen with 3-hydroxy-copalic acid and ACD51-acylation of phthalic anhydride with 3-hydroxy-copalic acid. ACD13B showed no cytotoxic potential in the THP-1 line when tested at the concentration of 2 ??M. The other substances did not present significant cytotoxicity at concentrations tested. In addition, ACD13B significantly inhibited TNF-?? production in LPS-stimulated THP-1 cells. In animals with induced pleurisy, ACD13B reduced the total number of leukocytes by 40% and the number of mononuclear cells at a dose of 100 mg/kg intraperitoneally (IP) by up to 50%. There was also inhibition of pIKB-??. ACD13B presented a lower gastrointestinal effect when compared to ibuprofen in mice. The docking study revealed the affinity and mode of interaction of the substance ACD13B in COX-2 and COX-1, identifying hydrophobic and hydrophilic interactions. The results indicate that the ibuprofen derivative with 3-hydroxy copalic acid is a prototype of an anti-inflammatory drug with a lower gastrointestinal effect than ibuprofen, which may be explored in the future for other functional and therapeutic properties. O ??leo de copa??ba oriundo de diversas esp??cies do g??nero copaifera tem sido utilizado h?? muitos anos, comercialmente, em virtude de sua poss??vel a????o modulat??ria sobre processos inflamat??rios. Estudos fitoqu??micos das esp??cies, que elucidam sua composi????o qu??mica, fracionada em sesquiterpenos e diterpenos, tem sido realizados. Apesar do ??leo bruto ser bastante estudado, o conhecimento do efeito de suas subst??ncias isoladas ?? escasso, e poucos s??o os estudos sobre modifica????es estruturais desses ??cidos diterp??nicos, buscando melhorar suas atividades biol??gicas. Neste sentido o presente trabalho teve como objetivos o isolamento dos ??cidos diterp??nicos majorit??rios presentes no ??leo de Copaifera multijuga: ??cidos cop??lico e 3-hidr??xi-cop??lico e Copaifera reticulata: ??cido hardw??ckiico, realizando-se modifica????es estruturais nos 2 (dois) primeiros, por rea????es qu??micas de baixo custo e otimizadas, bem como ensaios in vitro e in vivo da subst??ncia com melhor screening antiinflamat??rio e posteriormente estudo in silico. Ap??s o isolamento, as subst??ncias foram semissintetizadas e suas estruturas elucidadas por an??lise de RMN 1H e 13C, e por conseguinte, testadas em ensaio de viabilidade celular e teste de verifica????o da atividade de inibi????o do TNF-?? in vitro. O modelo in vivo de pleurisia/pleurite em camundongos balb-c foi realizado com a subst??ncia de melhor screening anti-inflamat??rio (ACD13B-esterifica????o do ibuprofeno com o ??cido 3-hidr??xi-cop??lico), assim como ensaio de express??o da prote??na IKB-??. Alem disso, o ensaio de gastrotoxicidade tamb??m foi procedido com a mesma subst??ncia. Os derivados semissintetizados foram: ACD4-epoxida????o do ??cido cop??lico, ACD13B-esterifica????o do ibuprofeno com o ??cido 3-hidr??xi-cop??lico e ACD51-acila????o do anidrido ft??lico com o ??cido 3-hidr??xi-cop??lico. O ACD13B n??o apresentou potencial citot??xico na linhagem THP-1 quando testado na concentra????o de 2 ??M. As demais subst??ncias n??o apresentaram citotoxicidade nas concentra????es testadas. Al??m disso, o ACD13B inibiu significativamente a produ????o de TNF-?? em c??lulas THP-1 estimuladas por LPS. Em animais com pleurisia induzida o ACD13B reduziu em torno de 40% o total de leuc??citos e em at?? 50% o n??mero de mononucleares na dose de 100 mg/Kg por via intraperitonial (IP). Houve tamb??m inibi????o da pIKB-??. O ACD13B apresentou menor efeito gastrot??xico quando comparado ao ibuprofeno em camundongos. O estudo de docking revelou a afinidade e o modo de intera????o da subst??ncia ACD13B em COX-2 e COX-1, identificando intera????es hidrof??bicas e hidrof??licas. Os resultados obtidos indicam que o derivado do ibuprofeno com o acido 3-hidr??xi cop??lico apresenta-se como um prot??tipo de f??rmaco anti-inflamat??rio com menor efeito gastrot??xico que o ibuprofeno, o qual pode ser futuramente explorado quanto a outras propriedades funcionais e terap??uticas.

Submitted by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2018-02-23T15:10:32Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Tese Parcial_Daniely S. P. Machado.pdf: 705039 bytes, checksum: e9d5012b6902da7185c89df1446b3a96 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2018-02-23T15:10:47Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Tese Parcial_Daniely S. P. Machado.pdf: 705039 bytes, checksum: e9d5012b6902da7185c89df1446b3a96 (MD5) Made available in DSpace on 2018-02-23T15:10:47Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Tese Parcial_Daniely S. P. Machado.pdf: 705039 bytes, checksum: e9d5012b6902da7185c89df1446b3a96 (MD5) Previous issue date: 2016-01-07 CAPES - Coordena????o de Aperfei??oamento de Pessoal de N??vel Superior 92-99103-4244 Functional food is the one presenting in its formulation one or more substances, which have proved to both nourish the organism and hold a beneficial function for it. Foods presented in capsules or pills constituted by edible pieces of fruits and vegetables which, have been submitted to drying or dehydration processing, must be assessed as being novel. Zingiber zerumbet L. Smith (ZZ), an important member of family Zingiberaceae, used traditionally in folk medicine throughout the world, especially in the south of Asia including India. This species is marketed worldwide, mostly through the manufacture of the shampoo elaborated from its flower and, it presents countless medicinal properties, such as anti-inflammatory, antitumor, anti-allergic, antipyretic and anti-platelet aggregation activities. Zerumbona, the major active component extracted from the plant rhizome is a highly therapeutic-powered substance. Under this context, the present work proposes to encapsulate the aqueous and hydroalcoholic extracts from the rhizome of ZZ for being utilized as functional food, therefore, it was necessary to assess some their medicinal and toxic activities. ZZ rhizomes were processed for attaining the gross extract (ZZGE), and ground and submitted to maceration for achieving hydroalcoholic extracts. Determination of the minerals held by rhizomes of ZZ was determined by the AOAC (1995) method. The phytochemical screening of the extracts was carried out through Mattos (1997) methodology. Thin Layer Chromatography (TLC) in silica gel utilized iodine, serum sulfate, UV 254nm and 365nm as developers. The test with Artemia salina according to Meyer (1982) was employed for the cytotoxicity assay. Mice were utilized for the acute toxicity test, and followed up for 14 days. The ethanol-induced gastric model was utilized to determine the anti-ulcer activity. The antinociceptive activity was ascertained through acetic acid-induced abdominal writhing, hot plate and hyperalgesia. The anti-inflammatory activity was verified through Paw edema test in rats and ear edema test in mice. From the preliminary results we obtained a extractive rate of 60g from ZZGE and 10g from ZZHE, in a percentile of 1% and 0.16%, respectively. The extracts hold a characteristic odor. In the determination of ZZ rhizomes, there is a larger quantity of iron (231 mg/kg). The ZZGE and ZZHE extracts phytochemical screening revealed the presence of the following substances: xanthones, flavonoids, phenols, tannins and alkaloids. The TLC of ZZGE indicated a spot developed in iodine with a retention factor of approximately 0.7 cm similar to that of Zerumbona (0.8) and ZZHE presented three spots developed in iodine and UV 254nm. In acute toxicity and Subchronic, one may assert that ZZGE and ZZHE extracts do not produce any toxicity or mortality in mice and rats, yet some dependent doses caused some significant. ZZGE and ZZHE presented the LC50 of 117.49 and 181.9??g???mL on the assays with Artemia salina. The extracts had a strong inhibitory effect against gastric lesions have gastroprotective effect. Assessment of abdominal writhing test demonstrated that ZZGE and ZZHE in the doses of 250 and 500 mg/kg produced a significant anti-nociceptive effect. In the hot plate test, the extracts showed to hold analgesic activity. The anti-inflammatory activity assessment showed it to be significant in physiological action in determined doses in models of anti-inflammatory pain brought about by chemical stimulus. According to their preliminary outcomes both ZZGE and ZZHE presented anti-nociceptive, anti-inflammatory and anti-ulcerous effects, and held no toxicity. Probably, these extracts may be encapsulated as functional food, thus introducing a differentiated product into the Market and contributing to the scientific studies on this valuable, medicinal plant. Alimento funcional ?? aquele que apresenta em sua formula????o uma ou mais subst??ncias que comprovadamente al??m de nutrir tenha uma fun????o ben??fica ao organismo. Alimentos apresentados em c??psula, comprimidos e tabletes, constitu??dos de partes comest??veis de frutas e vegetais submetidos a processamento de secagem ou desidrata????o, devem ser avaliados como novos alimentos. O Zingiber zerumbet L. Smith (ZZ) ?? um importante membro da fam??lia Zingiberaceae tem sido tradicionalmente usado na medicina popular em todo o mundo, especialmente no Sul asi??tico, incluindo a ??ndia. Essa esp??cie ?? divulgada internacionalmente, principalmente pela fabrica????o de xampu elaborado da flor e apresenta in??meras propriedades medicinais tais como: atividade anti-inflamat??rio, antitumoral, antial??rgica, antipir??tico e atividade anti-agrega????o plaquet??ria. A Zerumbona, o pr??ncipio ativo extra??do dos rizomas da planta ?? uma subst??ncia de alto poder terap??utico. Neste contexto, o presente trabalho prop??e o encapsulamento dos extratos aquoso e hidroalco??lico dos rizomas de ZZ, para as mesmas serem utilizadas como alimento funcional, portanto, foi necess??rio avaliar algumas atividades e tamb??m a toxicidade dos extratos. Para o extrato aquoso (EAZZ) os rizomas de ZZ foram processados, e para o extrato hidroalco??lico os rizomas triturados foram submetidos ao processo de macera????o. A determina????o de minerais dos rizomas de ZZ foi pelo m??todo da AOAC (1995). O screening fitoqu??mico dos extratos foi atrav??s da metodologia de Mattos (1997). A Cromatografia de camada delgada (CCD) em s??lica gel utilizando como reveladores o iodo, sulfato s??rico e UV 254nm e 365nm. Foram utilizados camundongos para o teste de toxicidade aguda sendo avaliados durante 14 dias, para o teste de citotoxicidade, o teste com Artemia salina segundo Meyer (1982). Para a atividade anti??lcera foi utilizado o modelo g??strico de indu????o por etanol em camundongos, e a atividade antinociceptiva foi verificada atrav??s dos testes de contor????o abdominal induzida por ??cido ac??tico, teste de placa quente e hiperalgesia. A atividade anti-inflamat??ria foi avaliado os Edema de pata em ratos e Edema de orelha em camundongos. Dos resultados preliminares, obteve-se um teor extrativo de 60g de EBZZ e 10g de EHZZ, em um percentual de 1% e 0,16%, respectivamente. Os extratos possuem um odor caracter??stico. Na determina????o de minerais dos rizomas de ZZ existe maior quantidade de ferro (231 mg/kg). O screening fitoqu??mico de EBZZ e EHZZ revelou a presen??a dos seguintes compostos: xantonas, flavon??ides, fen??is, taninos e alcal??ides. Na CCD do EBZZ indicou uma mancha revelada no iodo com o fator de reten????o de aproximadamente 0,7 cm semelhante ao da Zerumbona (0,8) e EHZZ apresentou tr??s manchas reveladas em iodo e UV 254nm. Na toxicidade aguda e subcr??nica, pode-se afirmar que os extratos EBZZ e EHZZ n??o produzem nenhuma toxicidade ou mortalidade em camundongos e ratos. EBZZ e EHZZ apresentaram uma CL50 de 117.49 e 181.9??g???mL no teste de citoxidade com Artemia salina . Os extratos tiveram um potente efeito inibit??rio contra les??es g??stricas, possuem efeito gastroprotetor. Na avalia????o do teste de contor????o abdominal pode-se constar que EBZZ e EHZZ nas doses de 250 e 500 mg/kg produziram um efeito anti-nocicept??vel significativo. No teste de placa quente e hiperalgesia os extratos demonstraram que possuem atividade analg??sica. A avalia????o da atividade anti-inflamat??ria foi significativa na a????o fisiol??gica em determinadas doses em modelos de dor inflamat??ria causada por est??mulo qu??mico. De acordo com os resultados preliminares de EBZZ e EHZZ ambos apresentaram efeitos antinocicept??vel, anti-inflamat??rio e anti-ulceroso, e n??o tiveram toxicidade, possivelmente os extratos podem ser encapsulados como alimento funcional introduzindo um produto biotecnol??gico diferenciado no mercado e contribuindo para os estudos cient??ficos desta valiosa planta medicinal.

Submitted by G??bia Leite (gabya.leite@gmail.com) on 2016-07-11T14:08:17Z No. of bitstreams: 1 Tese - Denise Morais Lopes Galeno.pdf: 1403594 bytes, checksum: 85b573886b109934233d6508fca06378 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2016-07-14T12:22:58Z (GMT) No. of bitstreams: 1 Tese - Denise Morais Lopes Galeno.pdf: 1403594 bytes, checksum: 85b573886b109934233d6508fca06378 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2016-07-14T12:28:44Z (GMT) No. of bitstreams: 1 Tese - Denise Morais Lopes Galeno.pdf: 1403594 bytes, checksum: 85b573886b109934233d6508fca06378 (MD5) Made available in DSpace on 2016-07-14T12:28:44Z (GMT). No. of bitstreams: 1 Tese - Denise Morais Lopes Galeno.pdf: 1403594 bytes, checksum: 85b573886b109934233d6508fca06378 (MD5) Previous issue date: 2014-06-13 CAPES - Coordena????o de Aperfei??oamento de Pessoal de N??vel Superior Galeno D.M.L. Effects of Eugenia punicifolia (Khunt ) DC related to metabolic syndrome in experimental models . Manaus, 2014. 133p. Thesis (Ph.D.) - Graduate Program Multi - Institutional Biotechnology, Federal University of Amazonas. The Eugenia punicifolia species is a plant popularly known as "pedra-ume-caa", widely distributed in the Amazon region. In the state of Amazonas, this species has been widely used in folk medicine to treat inflammatory and hyperglycemic disorders. However, studies involving other parameters found in the metabolic syndrome (MS) have not yet been elucidated. The MS is characterized by a complex disorder, represented by a number of cardiovascular risk factors involving central adiposity, hypertension, endothelial dysfunction and insulin resistance. Studies have shown that nutritional supplementation with plants that effectively address the pathogenic mechanisms of this syndrome represents an effective improvement for the problem. This project aim was to analyze biological activities in vitro and in vivo of the aqueous extract of the leaves of E. punicifolia (EEP), emphasizing the inhibitory activity digestive enzymes, angiotensin converting enzyme (ACE) inhibitor, antioxidants, hypolipemiant, cytotoxic and anti antihypertensive effects. Enzyme inhibition assays, as well as the determination of the ACE activity in Wistars rats serum were performed by the fluorometric method and the indirect tests of cell viability, antioxidants, determining the presence of flavonoids and phenolic compounds were performed by colorimetric assays. The evaluation of the EEP on blood pressure parameters was performed by using five experimental groups, which were randomly divided into: normotensive group (2R), the hypertensive group (2R1C), enalapril hypertensive group (ENAL), extract hypertensive group 150 mg/kg/day (EEP150) and extract hypertensive group 300 mg/kg/dia (EEP300). The effects of EEP in systolic pressure were acquired indirectly by tail plethysmography in conscious rats. Biochemical parameters were measured in rat???s plasma through appropriate kits. The study has shown, for the first time, that EEP exhibited scavenging activity on ABTS??+, DPPH??, and O2??- (IC50=10.5 ?? 1.2; 28.84 ?? 0.54 and 38.12 ?? 2.6 ??g/mL, respectively). The EEP also exhibited cellular antioxidant activity in vitro concentration-dependent manner, and did not show cell cytotoxic effects. Furthermore, the EEP inhibited ACE activity (70.8%) using the concentration of 0.3 mg/mL (IC50 of 24.3 ?? 0.7 mg/mL), and inhibited the activities of the enzymes ??-amylase (IC50 = 122.8 ?? 6.3 mg/mL), ??-glucosidase (IC50 = 2.9 ?? 0.1 mg/mL) and xanthine oxidase (IC50 = 23.5 ?? 2.6 mg/mL). Both the EEP150 (21.7%) and the EEP300 (16.8%) reduced systolic blood pressure. The hypotensive effect of EEP correlated with the reduction of plasma ACE activity and also reduced total cholesterol (48%), triglycerides (52%), VLDL (52%) and LDL (55%). In addition, the EEP wasn???t cause renal, hepatic or neurotoxic acute or chronic toxicity. The EEP showed antioxidants, hypotensive, hypolipidemic effects and digestive enzymes and ACE were inhibited. These activities are probably related to the presence of antioxidant such as phenols and flavonoids compounds present in the extract, which act by inhibiting key enzymes of carbohydrate metabolism and angiotensin converting enzyme. These findings may support further clinical studies to further evaluate the potential of EEP on the SM. Galeno, D.M.L. Efeitos da Eugenia punicifolia (Khunt) DC em modelos experimentais relacionados ?? S??ndrome Metab??lica. Manaus, 2014.133p. Tese (doutorado)- Programa de P??s-graduac??o Multi-Institucional em Biotecnologia da Universidade Federal do Amazonas A esp??cie Eugenia punicifolia ?? uma planta popularmente conhecida como "pedra-ume-ca?????, distribu??da amplamente na regi??o amaz??nica. No estado do Amazonas, essa esp??cie tem sido bastante utilizada na medicina popular para tratar dist??rbios hiperglic??micos e inflamat??rios. No entanto, estudos envolvendo outros par??metros encontrados na s??ndrome metab??lica (SM) ainda n??o foram elucidados. A SM se caracteriza por um transtorno complexo, representado por um conjunto de fatores de risco cardiovascular que envolve deposi????o central de gordura, hipertens??o arterial, disfun????o endotelial e resist??ncia ?? insulina. Estudos t??m evidenciado que a suplementa????o nutricional com plantas que atuem efetivamente com os mecanismos patog??nicos desta s??ndrome representa um potencial eficaz para o problema. Este projeto teve como objetivo analisar atividades biol??gicas in vitro e in vivo do extrato aquoso das folhas da E. punicifolia (EEP), enfatizando a atividade inibit??ria de enzimas digest??rias, enzima conversora de angiotensina (ECA), efeitos antioxidantes, hipolipemiantes, citot??xicos e anti-hipertensivos. Os ensaios de inibi????o enzim??tica, assim como a determina????o da atividade da ECA no soro de ratos wistars foram realizados utilizando um m??todo indireto fluorim??trico e os testes de viabilidade celular, antioxidantes, determina????o da presen??a de compostos fen??licos e flavonoides por meio de ensaios colorim??tricos. A avalia????o do EEP sobre os par??metros press??ricos foi realizada mediante a utiliza????o de cinco grupos experimentais, os quais foram divididos randomicamente em: Grupo normotenso (2R), Grupo hipertenso (2R1C), Grupo hipertenso enalapril (ENAL), Grupo hipertenso extrato 150 mg/kg/dia (EEP150) e Grupo hipertenso extrato 300 mg/kg/dia (EEP300). Os efeitos do EEP na press??o sist??lica foram adquiridos de forma indireta por plestimografia de cauda em ratos conscientes. Os par??metros bioqu??micos foram avaliados no plasma dos ratos por meio de kits apropriados. Nesse estudo foi mostrado, pela primeira vez, que o EEP apresentou atividade sequestradora de radicais livres nos testes de ABTS??+, DPPH?? e O2??- (CI50=10,5 ?? 1,2; 28,84 ?? 0,54; e 38,12 ?? 2,6 ??g/mL, respectivamente). O EEP tamb??m exibiu atividade antioxidante celular in vitro de maneira concentra????o-dependente, al??m de n??o evidenciar efeitos citot??xicos celulares. Ademais, o EEP inibiu 70,8% a atividade da ECA na concentra????o de 0,3 mg/mL (CI50 de 24 ?? 0,7 ??g/mL), inibiu as atividades das enzimas ??-amilase (CI50=122,8 ?? 6,3 ??g/mL), ??-glucosidase (CI50= 2,9 ?? 0,1 ??g/mL) e xantina oxidase (CI50= 23,5 ?? 2,6 ??g/mL). O EEP150 reduziu a press??o sist??lica em 21,7% e o EEP300 em 16,8%. O efeito hipotensor do EEP se correlacionou com a redu????o da atividade da ECA no plasma e tamb??m diminuiu o colesterol total (48%), triglicer??deos (52%), VLDL (52%) e LDL (55%). Al??m disso, o EEP n??o causou toxicidade cr??nica e nem aguda renal, hep??tica ou neurot??xica. O EEP mostrou atividades antioxidantes, hipotensores, hipolipemiantes e inibiu as enzimas digest??rias e a ECA. Essas atividades provavelmente estejam relacionadas ?? presen??a de compostos antioxidantes, como fen??is e flavonoides presentes no extrato, que atuam inibindo enzimas chaves do metabolismo dos carboidratos e a enzima conversora de angiotensina. Estes achados podem fundamentar estudos cl??nicos posteriores para melhor avaliar o potencial do EEP sobre a SM.

Made available in DSpace on 2015-04-11T13:38:30Z (GMT). No. of bitstreams: 1 Capa da Dissertacao Georgia.pdf: 241136 bytes, checksum: 26172cab25063b953eff0455c1729780 (MD5) Previous issue date: 2013-05-21 Conselho Nacional de Desenvolvimento Cient??fico e Tecnol??gico Lipid disorders are related to the development of cardiovascular and cerebrovascular diseases and therefore is considered an important risk factor that can be treated by drugs or even with changes in eating habits and physical. The result is a tucum?? regional physico-chemical features important for the treatment of dyslipidemia, such as substantial concentration of fibers. Thus, the present study aims to conduct in vivo study in rats untrained and trained on the use of tucum?? the Amazons in the treatment of diet-induced dyslipidemia. Was performed for both the induction of dyslipidemia followed by treatment with a base ration tucum?? with or without swimming. We used 40 male rats weighing about 350 grams. These animals were divided into 4 groups of 10 animals forming the Sedentary Control Group (SCG) the exercised control group (GCE), the Group Treatment Sedentary (GTS) and Treatment Group exercised (GTE). It is the body mass gain, feed intake, plasma concentrations of cholesterol, triglycerides, HDL-C, LDL-C, VLDL, total protein, and the hormones insulin and leptin. The GCE group showed a tendency to higher food consumption and the groups that consumed the flesh of the fruit tucum?? showed a trend to a higher body mass gain. The descriptive analysis showed that the groups that consumed the diet increased the fruit tucum?? showed a trend to a higher body mass gain. The GCE has a tendency to higher plasma cholesterol, as well as the GTE GTS and have higher absolute values for triglycerides and VLDL-C HDL lipids and LDL-C were shown to be elevated in the group that consumed tucum??. Through research it can be seen that the fruit tucum?? (Meyer Astrocatyum aculeatum) hypolipidemic has no effect, since the animals had elevated concentrations of lipid As dislipidemias est??o relacionadas ao desenvolvimento de doen??as cardiovasculares e cerebrovasculares e, portanto, ?? considerado um fator de risco importante que pode ser tratado atrav??s de f??rmacos ou mesmo com mudan??as nos h??bitos alimentares e f??sicos. O tucum?? ?? um fruto regional que apresenta caracter??sticas f??sico-qu??micas importantes para o tratamento das dislipidemias, como a concentra????o consider??vel de fibras. Diante disso, o presente estudo tem como objetivo realizar o estudo experimental in vivo em ratos Wistar sedent??rios e exercitados sobre o consumo de tucum?? do amazonas no tratamento da dislipidemia induzida por dieta. Para tanto foi realizado a indu????o da dislipidemia seguido do tratamento com ra????o a base de tucum?? associado ou n??o a nata????o. Foram utilizados 40 ratos machos pesando em m??dia 350 gramas. Esses animais foram divididos em 4 grupos com 10 animais formando o Grupo Controle Sedent??rio (GCS) o Grupo Controle Exercitado (GCE), o Grupo Tratamento Sedent??rio (GTS) e o Grupo Tratamento Exercitado (GTE). Verificou-se o ganho de massa corporal, consumo de ra????o, a concentra????o plasm??tica de colesterol, triglicer??deos, HDL-c, LDL-c, VLDL, prote??nas totais, al??m dos horm??nios insulina e leptina. O grupo GCE apresentou tend??ncia ao maior consumo alimentar e os grupos que consumiram a polpa do fruto tucum?? apresentaram uma tend??ncia ao maior ganho de massa corporal. A an??lise descritiva demonstrou que os grupos que consumiram a dieta acrescida do fruto tucum?? apresentaram uma tend??ncia ao maior ganho de massa corporal. O grupo GCE apresenta uma propens??o a maior concentra????o plasm??tica do colesterol, assim como os GTS e GTE apresentam maiores valores absolutos para os triglicer??deos e para o VLDL Os lip??dios HDL-c e LDL-c mostraram-se elevados nos grupos que consumiram tucum??. Atrav??s da pesquisa realizada pode-se observar que o fruto tucum?? (Astrocatyum aculeatum Meyer) n??o apresenta efeito hipolipid??mico, visto que os animais apresentaram concentra????es elevadas dos lip??deos

Submitted by Kamila Costa (kamilavasconceloscosta@gmail.com) on 2015-06-30T20:23:17Z No. of bitstreams: 1 Dissertacao-Deborah R de Freitas.pdf: 1243804 bytes, checksum: 3b6cf4267745ff21bfecaa822fb73c05 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2015-07-07T12:38:14Z (GMT) No. of bitstreams: 1 Dissertacao-Deborah R de Freitas.pdf: 1243804 bytes, checksum: 3b6cf4267745ff21bfecaa822fb73c05 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2015-07-07T12:42:00Z (GMT) No. of bitstreams: 1 Dissertacao-Deborah R de Freitas.pdf: 1243804 bytes, checksum: 3b6cf4267745ff21bfecaa822fb73c05 (MD5) Made available in DSpace on 2015-07-07T12:42:01Z (GMT). No. of bitstreams: 1 Dissertacao-Deborah R de Freitas.pdf: 1243804 bytes, checksum: 3b6cf4267745ff21bfecaa822fb73c05 (MD5) Previous issue date: 2013-02-25 CAPES - Coordena????o de Aperfei??oamento de Pessoal de N??vel Superior The present study aimed to investigate the influence of socioeconomic conditions, diet and prenatal care on the nutritional status of pregnant women attending a public maternity hospital in Manaus / AM in 2011. The sample was representative (n = 384) and the information was obtained in two flows: Primary - by interviewing random, using standardized forms for group nutrition CSAS/INPA, were investigated the insertion model socioeconomic and dietary patterns of mothers ??? Secondary, from the card of the pre nant o an ere obtained data re ardin to prenatal n con ultation , beginning of prenatal examination and anemia. The nutritional status before pregnancy and gestational were classified according to the recommendations of the System Food and Nutrition Surveillance ??? SISVAN. The project was initiated after approval by the Ethics Committee of the National Institute of Amazonian Research, INPA. Was used for tabulation of results the software Epi-Info version 7 with a significance level of 5%. The results evidenced that during the pre-pregnancy 5.7% of pregnant women presented with low weight, 58.6% suitable weight , 24.0% overweight and 11.7% obese. In the last consultation was observed that the percentage of underweight pregnant women evolved to 17.7%, suitable weight decreased 39.1%, increasing cases of overweight and obesity to 24.5% and 18.8% respectively. The average pre-pregnancy weight was 59.3 kg, 70.9 kg evolving to the last consultation. Among the socioeconomic variables investigated only the maternal e plo ent durin pre nanc o ed a tati ticall i ni icant relati e to t e nutritional tatu o pre nant o en p it re ard to t e odel o prenatal care a eri ied tati ticall i ni icant or t e ariable aternal a e n consultations in prenatal, abortion and parity (p 0.05). O presente estudo objetivou a investiga????o de influ??ncias das condi????es socioecon??micas, diet??ticas e da assist??ncia ao pr??-natal sobre o estado nutricional de gestantes atendidas em uma maternidade p??blica de Manaus/AM em 2011. A amostra foi representativa (n= 384) e as informa????es foram obtidas em dois fluxos?? Prim??rio ??? por entrevista aleat??ria, utilizando-se formul??rios padronizados pelo grupo de nutri????o da CSAS/INPA, foram investigados o modelo de inser????o socioecon??mico e padr??o diet??tico das m??es ??? Secund??rio, a partir do cart??o da gestante obtiveram-se dados referentes ao pr??-natal?? n?? de consultas, in??cio do pr??-natal e exame de anemia. O estado nutricional pr??-gestacional e gestacional, foram classificados de acordo com as recomenda????es do Sistema de Vigil??ncia Alimentar e Nutricionais ??? SISVAN. O projeto foi iniciado ap??s sua aprova????o pelo Comit?? de ??tica em Pesquisa do Instituto Nacional de Pesquisas da Amaz??nia- INPA. Utilizou-se para tabula????es dos resultados o software Epi-Info vers??o 7 com n??vel de signific??ncia de 5%. Os resultados evidenciaram que no per??odo pr??-gestacional 5,7% das gestantes apresentavam-se com baixo peso, 58,6% com peso adequado, 24,0% sobrepeso e 11,7% obesidade. Na ??ltima consulta verificou-se que o percentual de gestantes com baixo peso evoluiu para 17,7%, o peso adequado regrediu 39,1%, evoluindo os casos de sobrepeso e obesidade para 24,5% e 18,8% respectivamente. A m??dia de peso pr??-gestacional foi 59,3 kg, evoluindo para 70,9 kg na ??ltima consulta. Dentre as vari??veis socioecon??micas investigadas apenas o trabalho materno durante a gesta????o apresentou rela????o estatisticamente significativa em rela????o ao estado nutricional das gestantes (p< 0,05). No que se refere ao modelo de assist??ncia ao pr??-natal constatou-se rela????o estatisticamente significativa para as vari??veis: idade materna, n?? de consultas realizadas no pr??-natal, aborto e paridade (p< 0,05). Ao investigar a influ??ncia do padr??o alimentar na determina????o do estado nutricional das gestantes, percebe-se que o consumo di??rio de todos os componentes da dieta?? carboidratos 95,6%, prote??nas 86,4%, lip??deos 92,7%, vitaminas e minerais 57,9% foi expressivamente superior no grupo das gestantes com sobrepeso/ obesidade (p> 0,05).

Submitted by Kamila Costa (kamilavasconceloscosta@gmail.com) on 2015-07-30T20:30:54Z No. of bitstreams: 2 Disserta????o - Jamile L Freire.pdf: 613265 bytes, checksum: 6717901329ef990dbfa3295cede3d795 (MD5) ficha catalografica.pdf: 1849 bytes, checksum: c0de4bb17c675bdb8d657be247620a06 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2015-08-04T14:03:15Z (GMT) No. of bitstreams: 2 Disserta????o - Jamile L Freire.pdf: 613265 bytes, checksum: 6717901329ef990dbfa3295cede3d795 (MD5) ficha catalografica.pdf: 1849 bytes, checksum: c0de4bb17c675bdb8d657be247620a06 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2015-08-04T14:07:45Z (GMT) No. of bitstreams: 2 Disserta????o - Jamile L Freire.pdf: 613265 bytes, checksum: 6717901329ef990dbfa3295cede3d795 (MD5) ficha catalografica.pdf: 1849 bytes, checksum: c0de4bb17c675bdb8d657be247620a06 (MD5) Made available in DSpace on 2015-08-04T14:07:46Z (GMT). No. of bitstreams: 2 Disserta????o - Jamile L Freire.pdf: 613265 bytes, checksum: 6717901329ef990dbfa3295cede3d795 (MD5) ficha catalografica.pdf: 1849 bytes, checksum: c0de4bb17c675bdb8d657be247620a06 (MD5) Previous issue date: 2012-11-01 CAPES - Coordena????o de Aperfei??oamento de Pessoal de N??vel Superior Low Birth Weight (LBW), multifactorial event and implicated as a major contributor to the rise in infant mortality in developing countries. The objective of this study was to investigate the determinants of LBW. Cross-sectional study of descriptive population-based involved 384 mothers attended in a public hospital. Forms with closed questions were used on the socioeconomic, demographic condition of pregnancy and nutritional standard features, and anthropometric measurements of prenatal card. The results showed an incidence of LBW infants 6.5%, not registering this association influence of socioeconomic variables (p> 0.05). In relation to maternal characteristics, the number of consultations in prenatal care, the previous history of abortion and the type of delivery showed a statistically significant relationship in determining the BPN (p 0.05), and zero the occurrence of this event in mothers with older age (??? 35 years). Analysis of these results shows a low occurrence of LBW in the universe studied, however it reinforces the need to implement a program of assistance and guidance to more efficient prenatal care for pregnant women teens. O Baixo Peso ao Nascer (BPN), evento de etiologia multifatorial e incriminado como um dos principais respons??veis da eleva????o da mortalidade infantil nos pa??ses em desenvolvimento. O objetivo deste trabalho foi investigar os fatores determinantes do BPN. Estudo transversal de base populacional descritivo envolveu 384 pu??rperas atendidas em uma maternidade p??blica. Foram utilizados formul??rios com perguntas fechadas sobre a condi????o socioecon??mica, demogr??fica, caracter??sticas da gesta????o e padr??o nutricional, sendo as medidas antropom??tricas obtidas do cart??o do pr??-natal. A an??lise dos resultados evidenciou uma ocorr??ncia de RNBP de 6,5%, n??o se registrando nesta associa????o influencia das vari??veis socioecon??micas (p>0,05). Em rela????o ??s caracter??sticas maternas, o n??mero de consultas realizadas no pr??-natal, a hist??ria pregressa de aborto e o tipo de parto apresentaram rela????o estatisticamente significativa na determina????o do BPN (p0,05), sendo nula a ocorr??ncia deste evento nas m??es com idade mais avan??ada (??? 35 anos). A an??lise destes resultados evidencia uma baixa ocorr??ncia de BPN no universo estudado, entretanto refor??a a necessidade de implementa????o de um programa de assist??ncia e orienta????o ao pr??-natal mais eficiente para as gestantes adolescentes.

Submitted by Kamila Costa (kamilavasconceloscosta@gmail.com) on 2015-06-30T20:03:15Z No. of bitstreams: 1 Disserta????o- Bianca L Vargas.pdf: 978654 bytes, checksum: 5e8b0997366945157f9b6106f7221c08 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2015-07-07T12:20:38Z (GMT) No. of bitstreams: 1 Disserta????o- Bianca L Vargas.pdf: 978654 bytes, checksum: 5e8b0997366945157f9b6106f7221c08 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2015-07-07T12:25:12Z (GMT) No. of bitstreams: 1 Disserta????o- Bianca L Vargas.pdf: 978654 bytes, checksum: 5e8b0997366945157f9b6106f7221c08 (MD5) Made available in DSpace on 2015-07-07T12:25:12Z (GMT). No. of bitstreams: 1 Disserta????o- Bianca L Vargas.pdf: 978654 bytes, checksum: 5e8b0997366945157f9b6106f7221c08 (MD5) Previous issue date: 2012-08-27 CAPES - Coordena????o de Aperfei??oamento de Pessoal de N??vel Superior Currently, chronic diseases such as diabetes, cardiovascular disease (CVD) and cancer account for 60% of all deaths worldwide. Oxidative stress caused by the action of free radicals is involved in the pathogenesis of many chronic diseases such as CVD. Flavonoids and vitamin C are antioxidant nutrients found in fruits and vegetables, and its regular consumption is associated with decreased risk of developing chronic diseases. Camu-camu (Myrciaria dubia [Kunth] McVaugh) is a fruit from the Amazon region that has significant levels of antioxidants, especially vitamin C and flavonoids. This study aimed to evaluate the effect of dust from the pulp of camu-camu on glucose and lipid profile in young adults. The pulp of camu-camu was lyophilized and its nutritional composition was analyzed using the methods indicated by Instituto Adolpho Lutz for the centesimal composition and HPLC method for vitamin C. For the intervention, was conducted a not randomized, double-blind, controlled clinical trial with 18 volunteers of both genders, aged between 21 and 35 years. The volunteers were divided into two groups: 1) intervention group that received capsules of lyophilized camu-camu containing 320 mg of vitamin C; 2) control group that received capsules containing 320 mg of synthetic vitamin C. The ingestion of capsules was daily for 15 days and blood samples were collected and analyzed before and after the intervention. Statistical differences in the levels of vitamin C in plasma, fasting glucose and lipid profile were verified by means of Student's ???t??? test. The lyophilized camu-camu presented 20,310 mg of vitamin C/100g and 12.89 mg of flavonoids/100g. At the end of the intervention was reported a significant decrease in fasting glucose, total cholesterol and HDL-cholesterol (p 0.05). It was concluded that the capsules of camu-camu were more efficient, presenting hypolipidemic and hypoglycemic action in volunteers studied. These results demonstrate the potential benefit of vitamin C and camu-camu health, presenting the fruit as an excellent source of this vitamin. As doen??as cr??nicas, como diabetes, doen??as cardiovasculares (DCV) e c??ncer, representam atualmente 60% de todas as mortes no mundo. O estresse oxidativo, desencadeado pela a????o de radicais livres, est?? envolvido na patog??nese de in??meras doen??as cr??nicas, a exemplo as DCV. Alguns nutrientes presentes em frutas e verduras possuem a????o antioxidante, como ?? o caso dos flavon??ides e da vitamina C, e seu consumo regular est?? associado ?? diminui????o do risco de desenvolvimento de doen??as cr??nicas. O camu-camu [Myrciaria dubia (Kunth) McVaugh] ?? um fruto exclusivo da regi??o amaz??nica que possui teores significativos de antioxidantes, principalmente vitamina C e flavon??ides. O presente trabalho teve como objetivo avaliar o efeito do p?? da polpa de camu-camu sobre a glicemia e o perfil lip??dico de adultos jovens. A polpa do camu-camu foi liofilizada e sua composi????o nutricional foi analisada utilizando-se os m??todos indicados pelo Instituto Adolpho Lutz para a composi????o centesimal e o m??todo de CLAE para vitamina C. Para a interven????o, foi conduzido um ensaio cl??nico controlado, duplo-cego, em 18 volunt??rios de ambos os g??neros, com idades entre 21 e 35 anos. Os participantes foram divididos em dois grupos: 1) grupo interven????o, que recebeu c??psulas de camu-camu liofilizado contendo 320 mg de vitamina C; 2) grupo controle, que recebeu c??psulas contendo 320 mg vitamina C sint??tica. A ingest??o das c??psulas foi di??ria durante 15 dias e duas coletas de sangue foram procedidas, uma antes do in??cio do estudo e outra ao final. Foram verificadas as diferen??as estat??sticas nos n??veis de vitamina C no plasma, glicemia de jejum e perfil lip??dico por meio do Teste t de Student. O camu-camu liofilizado apresentou 20.310 mg de vitamina C/100g e 12,89 mg de flavon??ides/100g. Ao final da interven????o registrou-se queda significativa nos n??veis de glicemia de jejum, colesterol total e HDL (p0,05). Conclui-se que as c??psulas de camu-camu foram mais eficientes, apresentando a????o hipolipid??mica e hipoglicemiante nos volunt??rios estudados. Tais resultados demonstram o potencial ben??fico da vitamina C e do camu-camu ?? sa??de, apresentando o fruto como uma excelente fonte desta vitamina.

Submitted by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2016-02-11T18:44:24Z No. of bitstreams: 2 Disserta????o - Silmara da Cunha Pimentel.pdf: 1973075 bytes, checksum: 3b9949f43e0f7b653e8e8ccf7fca49c2 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2016-02-12T12:55:06Z (GMT) No. of bitstreams: 2 Disserta????o - Silmara da Cunha Pimentel.pdf: 1973075 bytes, checksum: 3b9949f43e0f7b653e8e8ccf7fca49c2 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2016-02-12T12:55:22Z (GMT) No. of bitstreams: 2 Disserta????o - Silmara da Cunha Pimentel.pdf: 1973075 bytes, checksum: 3b9949f43e0f7b653e8e8ccf7fca49c2 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Made available in DSpace on 2016-02-12T12:55:22Z (GMT). No. of bitstreams: 2 Disserta????o - Silmara da Cunha Pimentel.pdf: 1973075 bytes, checksum: 3b9949f43e0f7b653e8e8ccf7fca49c2 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2012-08-03 FAPEAM - Funda????o de Amparo ?? Pesquisa do Estado do Amazonas Dyslipidemia is a modification in blood levels of fat, one of the higher incidence of the pathologies in the world population, currently regarded as a public health concern. Some factors contribute greatly to increase cardiovascular diseases; among them, the lipid metabolism is one of most important. This factor is related to ingestion of lipid but the consumption of monounsaturated fatty acid (oleic) show neutral effect over cholesterolemy. However, it has been noticed that high oleic acid diets increase High Density Lipoprotein level (HDL-c) and may reduce the Low Density Lipoprotein level (LDL-c). The objective was to evaluate the Dyslipidemia Reduction Factor and compare the lipid profile and nutrition of the animals treated with the commercial diet supplemented and compare physicochemical fat and oil indexes using extra virgin olive oil and cupua??u fatty seed. In this comparative study was used experimentally fat of cupua??u seeds (Theobroma grandiflorum, Schum.) and olive oil (Olea europaea) extra virgin as a food supplementing in Wistar rats male youths. In the experiment were used six groups of eight animals and five of them were induced to dyslipidemia by use of monosodium glutamate. The applicability was 28 days which was provided usual feeding with supplemented of (0.1ml to 0.3ml) of one type of lipid in each group and gas chromatography and physicochemical analysis such as saponification, acidity, peroxide, and free fatty acid levels. So according to the procedures of gavage and oral route there was no difference, however between the lipidogram tests performed, Treatment with fat cupua??u seed was what achieved the best action of Dyslipidemia Reduction Factor, except for HDL cholesterol that there was no difference in any treatment. Results show that extra virgin olive oil and cupua??u fatty seed have same fatty acids; although, different amounts for these constituents. These results indicate that different quantities of same fatty acids on different levels may interfere on hipodislipidemic factor for cupua??u fatty seed diets. A dislipidemia ?? uma altera????o dos n??veis sangu??neos de gordura, uma das patologias com maior incid??ncia na popula????o mundial, considerada atualmente como uma preocupa????o para a sa??de p??blica. H?? fatores que contribuem para o aumento das doen??as cardiovasculares, entre estes as dislipidemias, mas o consumo de ??cido graxo monoinsaturado (oleico) exerce sobre a colesterolemia um efeito neutro. No entanto, tem se observado que as dietas ricas em ??cido oleico aumentam o HDL-c e podem reduzir o n??vel de LDL-c.. O objetivo foi avaliar o fator hipodislipid??mico e comparar o perfil lip??dico e nutricional dos animais tratados com dieta comercial suplementada. e comparar analiticamente os ??ndices f??sico-qu??micos de ??leos e gorduras de azeite de oliva extra virgem e semente de cupua??u, respectivamente. Neste estudo comparativo utilizou-se experimentalmente gordura de sementes de cupua??u (Theobroma grandiflorum) e azeite de oliva (Olea europaea) extra virgem como suplemento alimentar em ratos Wistar jovens do sexo masculino. No experimento foi utilizado seis grupos de oito animais e cinco deles foram induzidos ?? dislipidemia por uso de glutamato monoss??dico. A aplicabilidade foi de 28 dias onde foi oferecida alimenta????o usual com suplementa????o (0,1ml a 0,3ml) de um tipo de lip??dio em cada grupo tamb??m foi realizado estudo anal??tico, por cromatografia gasosa qualitativa e f??sico-qu??mico de 2 tipos de azeites de oliva extra virgem e dois lotes de gordura de semente do cupua??u. Portanto de acordo com os procedimentos de gavagem e via oral n??o houve diferen??a, por??m entre os exames realizados de colesterol total e triglicer??deos, o tratamento com gordura de semente de cupua??u foi o que obteve a melhor a????o hipodislipid??mica, Observou-se que azeite de oliva extra virgem e gordura de semente de cupua??u possuem o mesmo constituinte qu??mico, porem apresentam picos indicativos de quantidades diferentes Contudo verificou-se que as quantidades diferentes podem ser um indicativo de fator hipodislipidemico tamb??m para a gordura de cupua??u.

Submitted by Alisson Mota (alisson.davidbeckam@gmail.com) on 2015-07-21T19:45:42Z No. of bitstreams: 1 Tese - Vanderlan Santos Mota.doc: 5687808 bytes, checksum: 4c2e9bcb586f4406882237e819450320 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2015-07-23T18:19:04Z (GMT) No. of bitstreams: 1 Tese - Vanderlan Santos Mota.doc: 5687808 bytes, checksum: 4c2e9bcb586f4406882237e819450320 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2015-07-23T18:23:18Z (GMT) No. of bitstreams: 1 Tese - Vanderlan Santos Mota.doc: 5687808 bytes, checksum: 4c2e9bcb586f4406882237e819450320 (MD5) Made available in DSpace on 2015-07-23T18:23:18Z (GMT). No. of bitstreams: 1 Tese - Vanderlan Santos Mota.doc: 5687808 bytes, checksum: 4c2e9bcb586f4406882237e819450320 (MD5) Previous issue date: 2010-04-16 N??o Informada Recent studies in different countries have pointed the increase of the consumption of esteroides anabolizantes between practitioners of physical activities, and the damages to the health caused by its indiscriminate use. In Brazil, studies on the use of scarce anabolizantes are e, in this study, searched considera????es siginificativas to compose theoretical referencial on the alert subject and to the users of these products. It was examined perception of risk to the health, associated with the consumption of anabolizantes between practitioners of physical activities in the academies of the city of Manaus-AM. For in such a way, the collection proceeded from qualitative data and quantitative, such as etnografia, half-structuralized interviews and focal group with users of anabolizantes, using themselves applied analytical methods in the description of each stage of the experiments, had understood, beyond the laboratorias piss analyses, the analysis of chromatography and spectrometry, by means of statistical methods, analyzing the qui-square of the result for its validation. The produced data had described main used substances and the standards of use, between practitioners of physical activities in the city of Manaus pointing preponderant evidences to the masculine sex, as bigger substantial food consumer to the organism, amongst them: Calcium, Grain, Copper, Zinc, Iron, Manganese, B1 Vitamins, and the C, and Liquids, it is fallen again substance of Carboidratos, Fruits and Oil and Fat more for the consumption of the feminine sex. In the analyses physicist-chemistry of the urinas we find eighteen positive samples for forbidden substances, of these, twelve met in the results of the masculine sex. Amongst substances we have of the bigger evidence of pH Metiltestosterona and THC, Testosterone and DHEA, causers of pathological effect in the human metabolism. The results make in them to analyze that it has absence of real cares to the organic body, therefore the interviewed ones are cliente of males that such substances can cause. One reveals, thus, the desire of the ideal physical body to the standards taxes in the society, to this overlap to the risk of harmful collateral effect how much to the bad use of these substances. It is considered that more must be promoted the prevention and the orientation with regard to the anabolizantes use of esteroides next to this population. This study it intended to well more aim at the education of the anab??licos users of esteroides and the users in potential, looking for to inform involved with the practical sport and the porting one, therefore exactly with studies related to this still it is lacked of prevention. Estudos recentes em diferentes pa??ses t??m apontado o aumento do consumo de esteroides anabolizantes entre praticantes de atividades f??sicas, e os danos ?? sa??de causados pelo seu uso indiscriminado. No Brasil, estudos sobre o uso de anabolizantes s??o escassos e, neste estudo, buscou-se considera????es significativas para compor referencial te??rico sobre o tema e alerta aos usu??rios desses produtos. Examinou-se a percep????o de risco ?? sa??de, associada ao consumo de anabolizantes entre praticantes de atividades f??sicas nas academias da cidade de Manaus-AM. Para tanto, procedeu a coleta de dados qualitativos e quantitativos, tais como etnografia, entrevistas semi - estruturadas e grupo focal com usu??rios de anabolizantes, utilizando-se m??todos anal??ticos aplicados na descri????o de cada etapa dos experimentos, compreenderam, al??m das an??lises laboratoriais de urina, a an??lise de cromatografia e espectrometria, por meio de m??todos estat??sticos, analisando o qui-quadrado do resultado para sua valida????o. Os dados produzidos descreveram as principais subst??ncias utilizadas e os padr??es de uso, entre praticantes de atividades f??sicas na cidade de Manaus apontando evid??ncias preponderantes ao sexo masculino, como maior consumidor de alimentos subst??nciais ao organismo, dentre elas: C??lcio, Gr??o, Cobre, Zinco, Ferro, Mangan??s, Vitaminas B1, A e C, e L??quidos; recai-se a subst??ncia de Carboidratos, Frutas e ??leo e Gordura mais para o consumo do sexo feminino. Nas an??lises f??sico-qu??micas das urinas encontramos dezoito amostras positivas para subst??ncias proibidas; dessas, doze encontravam-se nos resultados do sexo masculino. Dentre as subst??ncias temos a de maior evid??ncia de pH, a Metiltestosterona e a THC, Testosterona e DHEA, causadoras de efeitos patol??gicos no metabolismo humano. Os resultados nos fazem analisar que h?? aus??ncia de cuidados reais ao corpo org??nico, pois os entrevistados s??o cientes dos males que tais subst??ncias podem ocasionar. Mostra-se, assim, o desejo do corpo f??sico ideal aos padr??es impostos na sociedade, a isso sobrep??em ao risco de danosos efeitos colaterais quanto ao mau uso dessas subst??ncias. Considera-se que se deve promover mais a preven????o e a orienta????o com rela????o ao uso de ester??ides anabolizantes junto a essa popula????o. Este estudo pretendeu visar bem mais ?? educa????o dos usu??rios de esteroides anab??licos e dos usu??rios em potencial, procurando informar os envolvidos com o esporte e a pr??tica desportiva, pois mesmo com estudos relacionados a isso ainda se carece de preven????o.

Made available in DSpace on 2015-04-20T12:31:39Z (GMT). No. of bitstreams: 1 - PDF.pdf: 156224 bytes, checksum: d8d6c26c25fefd22c60ea2b135c6dbc3 (MD5) Previous issue date: 2006-08-04 Funda????o de Amparo ?? Pesquisa do Estado do Amazonas Obesity is a disease that occurs due to the unbalance between the ingested energy and the spent energy to maintain the vital processes. Obesity has incresingly wrong and reached, nowadays, and epidemic level. It has also been considered the main public health issues in modern society. It is asociated with the rising risk of developing Diabetes Melliteus, cardiovascular diseases, dislipodemia, and several other chronic diseases. The diet based on fat maybe crucial reason to develop such diseases. The fishing also provides a large sort of food to thousands of local inhabitants nutrional development. Among the most well-known and consumed Amazon fish there is Tambaqui (Colossoma macropomum), Matrinx?? (Brycon cephalus) and Mapar?? (Hypophthalmus edentatus), which are all considered very fat species. And so, the main purpose of this essay is to analyse the effects of a diet based on Amazon fat fish (Mapar??, Matrinx?? and Tambaqui) on male mice lipydic metabolism from wistar blood line. They were feeded with Adlibitum for 14 days. To make it happen doses of total cholesterol and triglycerids, glycoses, proteins and plasma lipydic fraction were made. The rate of lypogenesis again in vivo has been measured on RET, EPI, FIG and CARC, by incorporating 3 H2O in lipy. Our results show that the enrichment of a diet based on Amazon fat fish has altered the mice lipydic metabolism, dicreased effectively the lipid plasmatic concentration (Col. TG, e LDL) and increased the HDL besides the lipolitic activity on fatty tissues has grown after 4 weeks of feeding. Admiting that the ingestion of hiperlipydic diets may have caused an aterogenic effect, mainly because they have reduced certain lipydic fractions that have been claimed to cause serious vascular problems. A obesidade ?? uma doen??a que ocorre como conseq????ncia do desequilibro no balan??o entre energia ingerida e a gasta para a manuten????o de processos vitais. A obesidade possui preval??ncia crescente, assumindo atualmente car??ter epid??mico, sendo considerada um dos principais problemas de sa??de p??blica na sociedade moderna. Ela est?? associada com o aumento do risco de desenvolve diabetes mellitus, doen??as cardiovasculares, dislipidemias e in??meras outras doen??as cr??nicas. As dietas ricas em lip??dios podem ser um fator determinante para o desenvolvimento de tais doen??as. A pesca contribui na oferta de alimentos e para o desenvolvimento nutricional de milhares de habitantes da regi??o. Dentre os mais conhecidos e consumidos peixes da Amaz??nia est??o o tambaqui (Colossoma macropomum), a matrinx?? (Brycon cephalus) e o mapar?? (Hypophthalmus edentatus), os quais s??o consideradas esp??cies bastante gordurosas. Desta forma objetivo deste trabalho foi avaliar os efeitos da dieta de peixes gordurosos (mapar??, matrinx?? e tambaqui) da Amaz??nia sobre o metabolismo lip??dico de ratos machos da linhagem wistar. Assim foram alimentados ad libitum por 30 dias. Para tanto foram realizadas as dosagens de colesterol total e triglic??rideos, glicose, prote??nas e fra????es lip??dicas do plasma. a taxa de lipog??nese de novo in vivo foi medida nos tecidos RET, EPI, FIG e CARC, por meio da incorpora????o de 3H2O em lip??dios. Nossos resultados mostram que o enriquecimento da dieta com peixes amaz??nicos gordurosos alterou o metabolismo lip??dico dos ratos, diminuindo de forma efetiva a concentra????o plasm??tica de lip??dios (Col. TG, e LDL) e aumentando o HDL. Al??m disso, a atividade lipol??tica nos tecidos adiposos aumentou ap??s 4 semanas de alimenta????o. Sugerindo que a ingest??o de dietas hiperlip??dicas pode ter efeito aterog??nico, principalmente, por diminu??rem determinadas fra????es lip??dicas, que tem sido responsabilizada por causar graves problemas vasculares.