Search

Your search keyword '"Carlos Antonio Couoh-May"' showing total 6 results
Start Over Author "Carlos Antonio Couoh-May"
6 results on '"Carlos Antonio Couoh-May"'

1. Whole genome analysis of Gram-negative bacteria using the EPISEQ CS application and other bioinformatic platforms

Author

Ulises Garza-Ramos, Nadia Rodríguez-Medina, Carlos Córdova-Fletes, Daira Rubio-Mendoza, Christopher J. Alonso-Hernández, Luis Esaú López-Jácome, Rao Morfín-Otero, Eduardo Rodríguez-Noriega, Fabián Rojas-Larios, María del Rosario Vázquez-Larios, Alfredo Ponce-de-Leon, Elena Victoria Choy-Chang, Rafael Franco-Cendejas, Bernardo Alfonso Martinez-Guerra, Cecilia Teresita Morales-de-La-Peña, Juan Pablo Mena-Ramírez, Eduardo López-Gutiérrez, Ricardo García-Romo, Bertha Ballesteros-Silva, Alejandro Valadez-Quiroz, Laura Karina Avilés-Benítez, José Manuel Feliciano-Guzmán, Talia Pérez-Vicelis, María del Consuelo Velázquez-Acosta, Cecilia Padilla-Ibarra, Laura Isabel López-Moreno, Reyna Edith Corte-Rojas, Carlos Antonio Couoh-May, María Angelina Quevedo-Ramos, Maribel López-García, Gabriela Chio-Ortiz, Mariana Gil-Veloz, Alejandro Molina-Chavarria, Javier Paul Mora-Domínguez, Daniel Romero-Romero, Francisco Javier May-Tec, and Elvira Garza-González

Subjects
EPISEQ, Pathogenwatch, Drug resistance, Carbapenem resistance, Whole genome sequencing, Microbiology, QR1-502
Abstract

ABSTRACT: Objectives: To determine genomic characteristics and molecular epidemiology of carbapenem non-susceptible Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii, and Pseudomonas aeruginosa from medical centres of Mexico using whole genome sequencing data analysed with the EPISEQⓇ CS application and other bioinformatic platforms. Methods: Clinical isolates collected from 28 centres in Mexico included carbapenem-non-susceptible K. pneumoniae (n = 22), E. coli (n = 24), A. baumannii (n = 16), and P. aeruginosa (n = 13). Isolates were subjected to whole genome sequencing using the Illumina (MiSeq) platform. FASTQ files were uploaded to the EPISEQⓇ CS application for analysis. Additionally, the tools Kleborate v2.0.4 and Pathogenwatch were used as comparators for Klebsiella genomes, and the bacterial whole genome sequence typing database was used for E. coli and A. baumannii. Results: For K. pneumoniae, both bioinformatic approaches detected multiple genes encoding aminoglycoside, quinolone, and phenicol resistance, and the presence of blaNDM-1 explained carbapenem non-susceptibility in 18 strains and blaKPC-3 in four strains. Regarding E. coli, both EPISEQⓇ CS and bacterial whole genome sequence typing database analyses detected multiple virulence and resistance genes: 20 of 24 (83.3%) strains carried blaNDM, 3 of 24 (12.4%) carried blaOXA-232, and 1 carried blaOXA-181. Genes that confer resistance to aminoglycosides, tetracyclines, sulfonamides, phenicols, trimethoprim, and macrolides were also detected by both platforms. Regarding A. baumannii, the most frequent carbapenemase-encoding gene detected by both platforms was blaOXA-72, followed by blaOXA-66. Both approaches detected similar genes for aminoglycosides, carbapenems, tetracyclines, phenicols, and sulfonamides. Regarding P. aeruginosa, blaVIM, blaIMP, and blaGES were the more frequently detected. Multiple virulence genes were detected in all strains. Conclusion: Compared to the other available platforms, EPISEQⓇ CS enabled a comprehensive resistance and virulence analysis, providing a reliable method for bacterial strain typing and characterization of the virulome and resistome.

Published
2023
Full Text
View/download PDF

2. Bacterial incidence and drug resistance from pathogens recovered from blood, cerebrospinal and pleural fluids in 2019–2020. Results of the Invifar network

Author

Elvira Garza-González, Adrian Camacho-Ortiz, Alfredo Ponce-de-Leon, Edgar Ortiz-Brizuela, Luis Esaú López-Jácome, Claudia Colin, Fabian Rojas-Larios, Oscar A. Newton-Sánchez, Gabriela Echaniz-Aviles, Maria Noemi Carnalla-Barajas, Araceli Soto, Paola Bocanegra-Ibarias, Ana María del Rocío Hernández-Dueñas, María del Consuelo Velázquez-Acosta, Laura Karina Avilés-Benítez, Juan Pablo Mena-Ramirez, Daniel Romero, Isela Mora-Jiménez, Margarita Alcaraz-Espejel, José Manuel Feliciano-Guzmán, Maribel López-García, Patricia Rodriguez-Zulueta, María Angelina Quevedo-Ramos, Cecilia Padilla-Ibarra, Carlos Antonio Couoh-May, Maria Carolina Rivera-Ferreira, Cecilia Teresita Morales-de-la-Peña, Hector Zubiate, Raúl Peralta-Catalán, Carlos Miguel Cetina-Umaña, Joaquin Rincón-Zuno, Maria Lucia Perez-Ricardez, Iris Yazmin Hernández-Cordova, Eduardo López-Gutiérrez, Mariana Gil, Efren Aguirre-Burciaga, Guadalupe Soledad Huirache-Villalobos, Scarlett Munoz, Nicolás Rogelio Eric Barlandas-Rendón, Enrique Bolado-Martinez, Luis Javier Quintanilla-Cazares, Abraham C. Gómez-Choel, Laura Lopez, Juan Carlos Tinoco, Rosa Areli Martínez-Gamboa, Alejandro Molina, Samuel Pavel Escalante-Armenta, Lizbeth Duarte, Luis Alberto Ruiz-Gamboa, Dulce Isabel Cobos-Canul, Dulce López, Irma Elena Barroso-Herrera-y-Cairo, Eduardo Rodriguez-Noriega, and Rayo Morfin-Otero

Subjects
Drug resistance, Blood stream infection, Cerebrospinal infection, Escherichia coli, INVIFAR, Medicine, Biology (General), QH301-705.5
Abstract

Background Antimicrobial resistance is a global concern. Analysis of sterile fluids is essential because microorganisms are defined as significant in most cases. Blood, cerebrospinal, and pleural fluids are frequently received in the microbiology lab because they are associated with considerable rates of morbi-mortality. Knowledge of epidemiology in these samples is needed to choose proper empirical treatments due to the importance of reducing selection pressure. Methods We used retrospective laboratory data of blood, CSF, and pleural fluid collected from patients in Mexico between 2019 and 2020. Each laboratory identified the strains and tested susceptibility using its routine methods. For Streptococcus pneumoniae, a comparative analysis was performed with data from the broth microdilution method. Results Forty-five centers participated in the study, with 30,746 clinical isolates from blood, 2,429 from pleural fluid, and 2,275 from CSF. For blood and CSF, Staphylococcus epidermidis was the most frequent. For blood, among gram negatives, the most frequent was Escherichia coli. Among Enterobacterales, 9.8% of K. pneumoniae were carbapenem-resistant. For S. pneumoniae, similar resistance percentages were observed for levofloxacin, cefotaxime, and vancomycin. For CSF, the most frequent gram-negative was E. coli. In Acinetobacter baumannii, carbapenem resistance was 71.4%. The most frequent species detected for pleural fluid was E. coli; in A. baumannii, carbapenem resistance was 96.3%. Conclusion Gram-negative bacteria, with E. coli most prevalent, are frequently recovered from CSF, blood, and pleural fluid. In S. pneumoniae, the routine, conventional methods showed good agreement in detecting resistance percentages for erythromycin, levofloxacin, and vancomycin.

Published
2023
Full Text
View/download PDF

3. Carbapenemase-Encoding Genes and Colistin Resistance in Gram-Negative Bacteria During the COVID-19 Pandemic in Mexico: Results from the Invifar Network

Author

Ulises Garza-Ramos, Jesús Silva-Sánchez, Luis Esaú López-Jácome, Melissa Hernández-Durán, Claudia Adriana Colín-Castro, Alejandro Sánchez-Pérez, Jonathan Rodríguez-Santiago, Rayo Morfín-Otero, Eduardo Rodriguez-Noriega, María-del-Consuelo Velázquez-Acosta, María Del Rosario Vázquez-Larios, José Manuel Feliciano-Guzmán, Fabián Rojas-Larios, Alfredo Ponce-De-Leon, Margarita Lozano-Garcia, Elena Victoria Choy-Chang, Eduardo López-Gutiérrez, Aarón Molina-Jaimes, Mariana Gil-Veloz, Reyna Edith Corte-Rojas, Ismelda López-Ovilla, Jose Luis Ramirez-Mis, Dora Elia Rodríguez-Balderas, Alejandro Molina-Chavarria, Cecilia Padilla-Ibarra, Maria Angelina Quevedo-Ramos, Christian Daniel Mireles-Dávalos, Nadia Rodríguez-Medina, Daira Rubio-Mendoza, Carlos Córdova-Fletes, Flora Cruz-López, Dilva Angelina Becerra-Montejano, Roberto Mercado-Longoria, Rebeca Thelma Martínez-Villarreal, Nicolás Rogelio Eric Barlandas-Rendón, Juan Pablo Mena-Ramírez, Carlos Antonio Couoh-May, Margarita Alcaraz-Espejel, César Adame-Alvarez, Lourdes Hernández-Vicente, and Elvira Garza-González

Subjects
Microbiology (medical), Pharmacology, Immunology, Microbiology
Abstract

In this study, we report the carbapenemase-encoding genes and colistin resistance in

Published
2022

4. Increment Antimicrobial Resistance During the COVID-19 Pandemic: Results from the Invifar Network

Author

Luis Esaú, López-Jácome, Diana, Fernández-Rodríguez, Rafael, Franco-Cendejas, Adrián, Camacho-Ortiz, María Del Rayo, Morfin-Otero, Eduardo, Rodríguez-Noriega, Alfredo, Ponce-de-León, Edgar, Ortiz-Brizuela, Fabian, Rojas-Larios, María Del Consuelo, Velázquez-Acosta, Juan Pablo, Mena-Ramírez, Patricia, Rodríguez-Zulueta, Enrique, Bolado-Martínez, Luis Javier, Quintanilla-Cazares, Laura Karina, Avilés-Benítez, Scarlett, Consuelo-Munoz, Elena Victoria, Choy-Chang, José Manuel, Feliciano-Guzmán, Carlos Antonio, Couoh-May, Eduardo, López-Gutiérrez, Aarón, Molina-Jaimes, Joaquín, Rincón-Zuno, Mariana, Gil-Veloz, Margarita, Alcaraz-Espejel, Reyna Edith, Corte-Rojas, Josué, Gómez-Espinosa, Víctor Antonio, Monroy-Colin, Cecilia Teresita, Morales-de-la-Peña, Efrén, Aguirre-Burciaga, Laura Isabel, López-Moreno, Rebeca Thelma, Martínez-Villarreal, Carlos Miguel, Cetina-Umaña, Mario, Galindo-Méndez, Gabriel Israel, Soto-Nieto, Dulce Isabel, Cobos-Canul, Martha Irene, Moreno-Méndez, Esmeralda, Tello-Gómez, Daniel, Romero-Romero, Sandra, Quintana-Ponce, Raúl, Peralta-Catalán, Alejandro, Valadez-Quiroz, Alejandro, Molina-Chavarría, Cecilia, Padilla-Ibarra, Irma Elena, Barroso-Herrera-Y-Cairo, Lizbeth Soraya, Duarte-Miranda, Dulce María, López-López, Samuel Pavel, Escalante-Armenta, Mónica Jazmín, Osorio-Guzmán, Maribel, López-García, Ulises, Garza-Ramos, Iván, Delgado-Enciso, and Elvira, Garza-González

Subjects
SARS-CoV-2, Drug Resistance, Multiple, Bacterial, COVID-19, Humans, Bacterial Infections, Microbial Sensitivity Tests, Mexico, Pandemics
Published
2021

5. The Evolution of Antimicrobial Resistance in Mexico During the Last Decade: Results from the INVIFAR Group

Author

Juan Pablo Mena-Ramirez, David Moncada-Barrón, María Noemí Carnalla-Barajas, Laura Karina Aviles-Benitez, Alina Aracely Rosales-García, Rafael Martínez-Miranda, Luis Carlos Armenta-Rodríguez, Adriana Mabel Coronado-Ramírez, Adrián Camacho-Ortiz, Daniel Romero-Romero, Efrén Aguirre-Burciaga, Cynthia Dennise Peña-Lopez, Carla Rocio Huerta-Baltazar, Domingo Sanchez-Francia, Elvira Garza-González, Arturo González-Moreno, Juan Manuel Barajas-Magallón, Carlos Miguel Cetina-Umaña, Gloria Edith Juárez-Velázquez, Gabriela Echániz-Aviles, Cecilia Teresita Morales-De-la-Peña, Rayo Morfin-Otero, Silvia Sida-Rodríguez, Fabian Rojas-Larios, Paola Bocanegra-Ibarias, Lourdes García-Mendoza, Rosa Elma Urbina-Rodríguez, Maricruz Gutierrez-Brito, Norma Alavez-Ramírez, María Magdalena Perez-Juárez, Edgar Ortiz-Brizuela, Cesar Adame-Álvarez, Ismelda López-Ovilla, Fidencio David Bello-Pazos, Rebeca Thelma Martínez-Villarreal, Gilberto Aguilar-Orozco, Joaquín Rincón-Zuno, Alfredo Ponce-de-León, Eduardo Rodríguez-Noriega, Alejandro Valadez-Quiroz, Araceli Soto-Noguerón, Marisol Manriquez-Reyes, Jorge Luis Canizales-Oviedo, Carlos Antonio Couoh-May, José Manuel Feliciano-Guzmán, Elena Victoria Choy-Chang, María Guadalupe Fong-Camargo, Joyarib Yanelli Maldonado-Anicacio, Eduardo López-Gutiérrez, Laura Isabel López-Moreno, Mariana Gil-Veloz, Rafael Franco-Cendejas, Nicolás Rogelio Eric Barlandas-Rendón, Luis Esau López-Jacome, Samantha Flores-Treviño, and Oscar Newton-Sánchez

Subjects
Microbiology (medical), medicine.medical_specialty, education, Immunology, Microbial Sensitivity Tests, Microbiology, 03 medical and health sciences, Antibiotic resistance, Internal medicine, Drug Resistance, Bacterial, medicine, Humans, Mexico, 030304 developmental biology, Retrospective Studies, Pharmacology, 0303 health sciences, Bacteria, 030306 microbiology, business.industry, Bacterial Infections, humanities, Anti-Bacterial Agents, body regions, business
Abstract

Background: Surveillance of antimicrobial resistance (AMR) requires an international approach with national and local strategies. Our aim was to summarize a retrospective 10-year report of antibiot...

Published
2020

6. A snapshot of antimicrobial resistance in Mexico. Results from 47 centers from 20 states during a six-month period

Author

Juan Pablo Mena-Ramirez, Adrián Camacho-Ortiz, Heidy Leticia Ostos-Cantú, Rayo Morfin-Otero, Carlos Miguel Cetina-Umaña, América Navarro-Rodríguez, Reyna E. Corte Rojas, Soraya Mendoza-Olazarán, Fabian Rojas-Larios, María-de-J. Ayala-Tarín, Rafael Martínez-Miranda, Consuelo Velázquez-Acosta, Gilberto Aguilar-Orozco, Ismelda López-Ovilla, Aurelio Galindo-Espinoza, Sara Arroyo-Escalante, Rafael Franco-Cendejas, Cecilia Teresita Morales-De-la-Peña, Joaquín Rincón-Zuno, Joyarit Y. Maldonado-Anicacio, Laura Karina Aviles-Benitez, Alina Aracely Rosales-García, Juan Manuel Barajas-Magallón, Jesus Silva-Sanchez, Jorge Luis Canizales-Oviedo, Efrén Aguirre-Burciaga, Gloria Edith Juárez-Velázquez, Aaron Molina-Jaimes, Elvira Garza-González, Alfredo Ponce-de-León, Domingo Sanchez-Francia, Javier P. Mora, Mabel Coronado-Ramírez, José Manuel Feliciano-Guzmán, Raúl Rivera-Garay, Abraham Gomez-Choel, Bertha A. Pérez-Vega, Dulce Isabel Cobos-Canul, Juan L. Jaime-Sanchez, Silvia Sida-Rodríguez, Paola Bocanegra-Ibarias, Luis J. Quintanilla, Cynthia Dennise Peña-Lopez, Samantha Flores-Treviño, Andrés Martínez-Ramirez, Carlos Antonio Couoh-May, Alejandra Arévalo-Mejía, Martha Irene Moreno-Méndez, Víctor A. Monroy-Colin, Elda G. Vázquez Narvaez, Daniel Romero-Romero, and Eduardo Rodríguez-Noriega

Subjects
Male, 0301 basic medicine, Research Facilities, Drug resistance, Pathology and Laboratory Medicine, Geographical locations, Antibiotics, Drug Resistance, Multiple, Bacterial, Klebsiella, Epidemiology, Prevalence, Medicine and Health Sciences, Multidisciplinary, Acinetobacter, Antimicrobials, Drugs, Pseudomonas Aeruginosa, Bacterial Pathogens, Medical Microbiology, Medicine, Female, Pathogens, Research Laboratories, Research Article, medicine.medical_specialty, Science, 030106 microbiology, Research and Analysis Methods, Microbiology, 03 medical and health sciences, Antibiotic resistance, Microbial Control, Pseudomonas, Environmental health, Gram-Negative Bacteria, Escherichia coli, medicine, Humans, Mexico, Microbial Pathogens, Retrospective Studies, Pharmacology, Bacteria, business.industry, Organisms, Biology and Life Sciences, biochemical phenomena, metabolism, and nutrition, 030104 developmental biology, Multicenter study, Antibiotic Resistance, North America, Antimicrobial Resistance, People and places, Gram-Negative Bacterial Infections, business, Software, Government Laboratories
Abstract

Aim We aimed to assess the resistance rates of antimicrobial-resistant, in bacterial pathogens of epidemiological importance in 47 Mexican centers. Material and methods In this retrospective study, we included a stratified sample of 47 centers, covering 20 Mexican states. Selected isolates considered as potential causatives of disease collected over a 6-month period were included. Laboratories employed their usual methods to perform microbiological studies. The results were deposited into a database and analyzed with the WHONET 5.6 software. Results In this 6-month study, a total of 22,943 strains were included. Regarding Gram-negatives, carbapenem resistance was detected in ≤ 3% in Escherichia coli, 12.5% in Klebsiella sp. and Enterobacter sp., and up to 40% in Pseudomonas aeruginosa; in the latter, the resistance rate for piperacillin-tazobactam (TZP) was as high as 19.1%. In Acinetobacter sp., resistance rates for cefepime, ciprofloxacin, meropenem, and TZP were higher than 50%. Regarding Gram-positives, methicillin resistance in Staphylococcus aureus (MRSA) was as high as 21.4%, and vancomycin (VAN) resistance reached up to 21% in Enterococcus faecium. Acinetobacter sp. presented the highest multidrug resistance (53%) followed by Klebsiella sp. (22.6%) and E. coli (19.4%). Conclusion The multidrug resistance of Acinetobacter sp., Klebsiella sp. and E. coli and the carbapenem resistance in specific groups of enterobacteria deserve special attention in Mexico. Vancomycin-resistant enterococci (VRE) and MRSA are common in our hospitals. Our results present valuable information for the implementation of measures to control drug resistance.

Published
2019

Catalog

Books, media, physical & digital resources
See catalog results