1. Ovine papillomavirus type 3 virus-like particle-based tools for diagnosis and detection of infection.
- Author
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Cacciotto C, Dore GM, Cubeddu T, Burrai GP, Anfossi AG, Antuofermo E, Varoni MV, Demontis MP, Zobba R, Pittau M, Müller M, and Alberti A
- Subjects
- Animals, Sheep, Carcinoma, Squamous Cell diagnosis, Carcinoma, Squamous Cell virology, Baculoviridae, Antibodies, Monoclonal immunology, Immunohistochemistry methods, Papillomaviridae immunology, Papillomavirus Infections diagnosis, Papillomavirus Infections virology, Antibodies, Viral blood, Antibodies, Viral immunology, Sheep Diseases diagnosis, Sheep Diseases virology, Enzyme-Linked Immunosorbent Assay methods
- Abstract
The design of prophylactic and diagnostic tools specific to animal papillomaviruses is hampered by the difficulties of viral in vitro manipulation and by the scarce availability of dedicated biotechnological tools. This paper reports the production of Ovine Papillomavirus 3 (OaPV3)-based virus-like particles (OaPV3-VLPs) in the baculovirus system and their use to investigate host humoral immune response through the establishment of an indirect ELISA test., Polyclonal sera and monoclonal antibodies were generated against OaPV3-VLPs, and their isotype and reactivity were determined. Additionally, antibodies allowed OaPV3 detection in ovine squamous cell carcinoma (SCC) samples by immunohistochemistry. Results encourage the standardization of OaPV3-specific prophylactic and serological diagnostic tools, and open new perspectives for the study of host-viral interaction and SCC development., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2024
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