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4. Significance of P16INK4A hypermethylation gene in primary head/neck and colorectal tumors: it is a specific tissue event? Results of a 3-year GOIM (Gruppo Oncologico dellʼItalia Meridionale) prospective study

Author

Agnese, V., Corsale, S., Calò, V., Augello, C., Bruno, L., Calcara, D., Crosta, A., Rodolico, V., Rinaldi, G., Cicero, G., Latteri, F., Agrusa, A., Morello, V., Adamo, V., Altavilla, G., Fede, G. Di, Fiorentino, E., Grassi, N., Latteri, M. A., Valerio, M. R., Tomasino, R. M., Colucci, G., Bazan, V., and Russo, A.

Published
2006

5. Downregulated expression of Cdc25A gene in MCF-7 breast cancer cell

Author

CORSINI, Lidia Rita, FANALE, Daniele, D'ANDREA, Aleco, LA PAGLIA, Laura, Calcara, D, AMODEO, Valeria, TERRASI, Marianna, INSALACO, Lavinia, Perez, M, CIMINO, Sybilla, BRUNO, Loredana, Calò, V, AGNESE, Valentina, SCHIRO', Valentina, BRONTE, Giuseppe, RIZZO, Sergio, FEDERICO, Mario, Symonds, CE, GRASSI, Nello, PANTUSO, Gianni, FRAZZETTA, Michele, BAZAN, Viviana, RUSSO, Antonio, Corsini, LR, Fanale, D, D'andrea, A, La Paglia, L, Calcara, D, Amodeo, V, Terrasi, M, Insalaco, L, Perez, M, Cimino, S, Bruno, L, Calò, V, Agnese, V, Schirò, V, Bronte, G, Rizzo, S, Federico, M, Symonds, CE, Grassi, N, Pantuso, G, Frazzetta, M, Bazan, V, and Russo, A

Subjects
breast cancer
Published
2009

6. Q356R and S1512I are BRCA1 variants that may be associated with breast cancer in a Sicilian family

Author

Augello, C, Bruno, L, Calò, V, Agnese, V, Corsale, S, Gregorio, V, Cascio, S, Gullo, A, Gargano, G, Barbera, F, LA PAGLIA, L, Terrasi, M, Schirò, V, Calcara, D, Adamo, Barbara, Morello, V, Tomasino, R. M., Bazan, V, Russo, A., AUGELLO C, BRUNO L, CAL V, AGNESE V, CORSALE S, GREGORIO V, CASCIO S, GULLO A, GARGANO G, BARBERA F, LA PAGLIA L, TERRASI M, SCHIRO V, CALCARA D, ADAMO B, MORELLO V, TOMASINO RM, BAZAN V, and RUSSO A

Published
2006

7. Ductal lavage: a valid method of risk assessment and of early diagnosis in breast cancer

Author

Calcara, D., Gregorio, V., Corsale, S., Calo, V., Agnese, V., Augello, C., Bruno, L., Gargano, G., Gullo, A., La Paglia, L., Schiro, V., Terrasi, M., Crosta, A., Badalamenti, G., Fricano, S., Napoli, L., Antonio Giordano, Tomasino, Rm, Morello, V., Daniele, E., Chiarini, A., Martorana, A., Gebbia, N., Bazan, V., Russo, A., CALCARA D, GREGORIO V, CORSALE S, CALO V, AGNESE V, AUGELLO C, BRUNO L, GARGANO G, GULLO A, LA PAGLIA L, SCHIRO, TERRASI M, CROSTA A, BADALAMENTI G, FRICANO S, NAPOLI L, GIORDANO A, TOMASINO RM, MORELLO V, DANIELE E, CHIARINI A, MARTORANA A, GEBBIA N, BAZAN V, and RUSSO A

Published
2006

12. Significance of P16INK4Ahypermethylation gene in primary head/neck and colorectal tumors: it is a specific tissue event? Results of a 3-year GOIM (Gruppo Oncologico dell'Italia Meridionale) prospective study

Author

Agnese, V., Corsale, S., Calò, V., Augello, C., Bruno, L., Calcara, D., Crosta, A., Rodolico, V., Rinaldi, G., Cicero, G., Latteri, F., Agrusa, A., Morello, V., Adamo, V., Altavilla, G., Di Fede, G., Fiorentino, E., Grassi, N., Latteri, M.A., Valerio, M.R., Tomasino, R.M., Colucci, G., Bazan, V., and Russo, A.

Abstract

Methylation of the p16 promoter is one of the most frequent mechanisms of gene inactivation; its incidence is extremely variable according to the type of tumor involved. Our purpose was to analyze the hypermethylation of the p16 promoter in laryngeal squamous cell carcinomas (LSCC), salivary gland (SG) tumors and in colorectal cancer (CRC), to detect any possible association with the clinicopathological features and to determine the prognostic significance of the p16 gene in the tumors analyzed.

Published
2006
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13. ASTENOZOOSPERMY AND MITOCHONDRIAL DNA

Author

CARRA, Elena, RINALDI, Anna Maria, GERARDI ELIANA, BARBARA SALERNO, CARRA E, CALCARA D, GERARDI E, RINALDI AM, CARRA, E, GERARDI, E, SALERNO, B, RINALDI, AM, SALERNO B, CALCARA, D, CARRA ELENA, GERARDI ELIANA, and BARBARA SALERNO

Published
2005

14. BRCA1 and BRCA2 germline mutations in sicilian breast and/or ovarian cancer families and their association with familial profiles

Author

Calo, V, Piazza, D, Symonds, CE, BRUNO, Loredana, LA PAGLIA, Laura, SCHIRO', Valentina, AGNESE, Valentina, CALCARA, Donatella, CIMINO, Sybilla, FANALE, Daniele, D'ANDREA, Aleco, CORSINI, Lidia Rita, AMODEO, Valeria, RIZZO, Sergio, TERRASI, Marianna, BRONTE, Giuseppe, BRUNO, Dario, FIORENTINO, Francesco Paolo, GRASSI, Nello, PANTUSO, Gianni, FRAZZETTA, Michele, FEDERICO, Mario, BAZAN, Viviana, RUSSO, Antonio, Calo, V, Bruno, L, La Paglia, L, Schirò, V, Agnese, V, Calcara, D, Cimino, S, Fanale, D, D'andrea, A, Corsini, LR, Amodeo, V, Rizzo, S, Terrasi, M, Bronte, G, Bruno, D, Piazza, D, Fiorentino, FP, Grassi, N, Pantuso, G, Frazzetta, M, Symonds, CE, Federico, M, Bazan, V, and Russo, A

Subjects
breast cancer, ovarian cancer, germline mutation
Published
2009

15. BRCA1 and BRCA2 variants of uncertain clinical significance and their implications for genetic counseling

Author

BRUNO, Loredana, Calò, V, Schirò, V, LA PAGLIA, Laura, AGNESE, Valentina, CALCARA, Donatella, CIMINO, Sybilla, FANALE, Daniele, D'ANDREA, Aleco, CORSINI, Lidia Rita, AMODEO, Valeria, RIZZO, Sergio, TERRASI, Marianna, BRONTE, Giuseppe, BRUNO, Dario, Piazza, D, Symonds, ES, FEDERICO, Mario, FIORENTINO, Francesco Paolo, GRASSI, Nello, PANTUSO, Gianni, FRAZZETTA, Michele, BAZAN, Viviana, RUSSO, Antonio, Bruno, L, Calò, V, Schirò, V, La Paglia, L, Agnese, V, Calcara, D, Cimino, S, Fanale, D, D'Andrea, A, Corsini, LR, Amodeo, V, Rizzo, S, Terrasi, M, Bronte, G, Bruno, D, Piazza, D, Symonds, ES, Federico, M, Fiorentino, FP, Grassi, N, Pantuso, G, Frazzetta, M, Bazan, V, and Russo, A

Subjects
genetic counseling, germline mutation
Published
2009

16. Molecular analysis of TP53, Ki-Ras and P16 methylation status in tissue and plasma of subjects affected by gastrointestinal cancer

Author

SCOLARO, Laura, AGNESE, Valentina, AUGELLO, Claudia, CASCIO, Sandra, BARBERA, Floriana, GREGORIO, Valter, CALCARA, Donatella, SCHIRO', Valentina, TERRASI, Marianna, FANALE, Daniele, FERLA, Rita, FIORENTINO, Francesco Paolo, FODDAI, Elena, DI GAUDIO, Francesca, CAL V, BRUNO L, GARGANO G, LA PAGLIA L, GRACEFFA G, BAZAN V. AND RUSSO A., SCOLARO L, AGNESE V, CAL V, BRUNO L, AUGELLO C, CASCIO S, GARGANO G, BARBERA F, GREGORIO V, CALCARA D, LA PAGLIA L, SCHIRO' V, TERRASI M, FANALE D, FERLA R, FIORENTINO FP, FODDAI E, DI GAUDIO F, GRACEFFA G, and BAZAN V AND RUSSO A

Published
2007

17. Aplotype analysis in four sicilian families with 5083del19bp-BRCA1

Author

SCHIRO', Valentina, FERLA, Rita, CALO', Valentina, BRUNO L, AGNESE, Valentina, CASCIO, Sandra, GARGANO G, BARBERA, Floriana, LA PAGLIA L, TERRASI, Marianna, CALCARA, Donatella, FANALE, Daniele, FIORENTINO, Francesco Paolo, SCOLARO, Laura, GRACEFFA, Giuseppa, DI GAUDIO, Francesca, BAZAN, Viviana, RUSSO, Antonio, FODDAI, Elena, SCHIRO V, FERLA R, CALÒ V, BRUNO L, AGNESE V, CASCIO S, GARGANO G, BARBERA F, LA PAGLIA L, TERRASI M, CALCARA D, FANALE D, FIORENTINO FP, SCOLARO L, GRACEFFA G, DI GAUDIO F, BAZAN V, Russo, A., and Foddai, E.

Subjects
aplotype analysis
Published
2007

18. BRCA 1/2 GENES MUTATIONAL SCREENING IN SICILIAN BREAST AND/OR OVARIAN CANCER FAMILIES

Author

SCHIRO', Valentina, FERLA, Rita, CALO', Valentina, AGNESE, Valentina, CASCIO, Sandra, BARBERA, Floriana, TERRASI, Marianna, CALCARA, Donatella, FANALE, Daniele, FIORENTINO, Francesco Paolo, SCOLARO, Laura, DI GAUDIO, Francesca, BAZAN, Viviana, FODDAI, Elena, BRUNO L, GARGANO G, LA PAGLIA L, GRACEFFA G, RUSSO, Antonio, SCHIRÒ V, FERLA R, CALÒ V, BRUNO L, AGNESE V, CASCIO S, GARGANO G, BARBERA F, LA PAGLIA L, TERRASI M, CALCARA D, FANALE D, FIORENTINO F, SCOLARO L, GRACEFFA G, DI GAUDIO F, BAZAN V, RUSSO A, and Foddai, E.

Published
2007

19. Analysis of TP53, Ki-Ras and P16INK4A promoter methylation as potential prognostic factors in patients with colorectal cancer

Author

SCOLARO, Laura, AGNESE, Valentina, CAL V, BRUNO L, AUGELLO, Claudia, CASCIO, Sandra, GARGANO G, BARBERA, Floriana, GREGORIO, Valter, CALCARA, Donatella, LA PAGLIA L, SCHIRO', Valentina, TERRASI, Marianna, FANALE, Daniele, FERLA, Rita, FIORENTINO, Francesco Paolo, FODDAI, Elena, DI GAUDIO, Francesca, GRACEFFA, Giuseppa, BAZAN, Viviana, RUSSO, Antonio, SCOLARO L, AGNESE V, CAL V, BRUNO L, AUGELLO C, CASCIO S, GARGANO G, BARBERA F, GREGORIO V, CALCARA D, LA PAGLIA L, SCHIRO' V, TERRASI M, FANALE D, FERLA R, FIORENTINO FP, FODDAI E, DI GAUDIO F, GRACEFFA G, BAZAN V, and RUSSO A

Subjects
Analysis,TP53, Ki-Ras, P16INK4A, colorectal cancer
Published
2007

20. Genotype analysis of colorectal carcinomas through laser pressare catapulting (LPC)

Author

FANALE, Daniele, AGNESE, Valentina, AUGELLO, Claudia, CASCIO, Sandra, BARBERA, Floriana, GREGORIO, Valter, CALCARA, Donatella, SCHIRO', Valentina, TERRASI, Marianna, FERLA, Rita, FIORENTINO, Francesco Paolo, SCOLARO, Laura, FODDAI, Elena, DI GAUDIO, Francesca, GRACEFFA, Giuseppa, BAZAN, Viviana, RUSSO, Antonio, CAL V, BRUNO L, GARGANO G, LA PAGLIA L, FANALE D, AGNESE V, CAL V, BRUNO L, AUGELLO C, CASCIO S, GARGANO G, BARBERA F, GREGORIO V, CALCARA D, LA PAGLIA L, SCHIRO' V, TERRASI M, FERLA R, FIORENTINO FP, SCOLARO L, FODDAI E, DI GAUDIO F, GRACEFFA G, BAZAN, V, and RUSSO, A

Subjects
Kinase inhibitor, Cancer treatment, Mitosi, Aurora-A, Small molecule
Abstract

Recently, new chemotherapy agents which target the non-structural components of mitosis have been developed. An important protein involved in several mitotic phases is the Aurora-A protein. By means of the phosphorylation of different substrates, Aurora-A regulates the correct development of the various phases of mitosis. The kinase activity of this protein makes Aurora-A an excellent candidate as an oncogene. The first data of Aurora-A involvement in cancer regarded the identification of Aurora-A overexpression in primary breast and colon tumour samples. With regard to the predictive role of Aurora-A, it has been shown that its overexpression disrupts the spindle checkpoint activated by paclitaxel (Taxol) or nocodazole treatment, thus inducing the cells to become resistant to these drugs. The development therefore of small molecules with an Aurora-A inhibition function may make it possible to reduce or block the oncogenic activity of Aurora-A and in addition may improve the survival of oncological patients showing resistance to paclitaxel or nocodazole treatment. Three novel Aurora kinase inhibitors have recently been described - Hesperadin, ZM447439 and VX-680. All these three drugs have been designed to target the ATP-binding site of Aurora kinase, so they inhibit all three Aurora kinase family members showing a similar phenotype when tested in cell-based assays. Among these three different molecules, VX-680 has shown promising results in in vitro and in vivo studies. In conclusion, it is clear that we are entering a new era in anti-mitotic therapy with the identification and now clinical translation of new targets in mitosis beyond tubulin but many questions remain with regard to Aurora function. © 2007 European Society for Medical Oncology.

Published
2007

21. TP53, H-K-RAS, P16INK4A GENE MOLECULAR ANALYSIS IN SALIVARY GLAND TUMORS

Author

AGNESE, Valentina, AUGELLO, Claudia, GREGORIO, Valter, CAMMARERI, Patrizia, GULLO, Arianna, CALO', Valentina, BRUNO, Loredana, SCHIRO', Valentina, TERRASI, Marianna, DANIELE, Elio, CASCIO, Sandra, MORELLO, Vincenza, TOMASINO, Rosa Maria, BUSCEMI, Maria, GERBINO, Aldo, BAZAN, Viviana, RUSSO, Antonio, SISTO, Pasqua Sandra, RESTIVO, Salvatore, CORSALE S, GARGANO G, FIORENTINO FP, AGNESE V, CALÒ V, BRUNO L, AUGELLO C, CASCIO S, BARBERA F, GARGANO G, GREGORIO V, CALCARA D, LA PAGLIA L, SCHIRÒ V, TERRASI M, FANALE D, FERLA R, SCOLARO L, FODDAI E, DI GAUDIO F, GRACEFFA G, BAZAN V, RUSSO A, CAMMARERI P, GULLO A, CALO' V, CORSALE S, SCHIRO' V, DANIELE E, MORELLO V, TOMASINO RM, BUSCEMI M, GERBINO A, Sisto, P.S., and Restivo, S.

Published
2007

22. BRCA1/BRCA2 genes mutational screening in Sicilian breast and/or ovarian cancer families

Author

SCHIRO', Valentina, FERLA, Rita, CAL V, BRUNO L, AGNESE, Valentina, CASCIO, Sandra, GARGANO G, BARBERA, Floriana, LA PAGLIA L, TERRASI, Marianna, CALCARA, Donatella, FANALE, Daniele, FIORENTINO, Francesco Paolo, SCOLARO, Laura, GRACEFFA, Giuseppa, DI GAUDIO, Francesca, BAZAN, Viviana, SCHIRO V, FERLA R, CAL V, BRUNO L, AGNESE V, CASCIO S, GARGANO G, BARBERA F, LA PAGLIA L, TERRASI M, CALCARA D, FANALE D, FIORENTINO F, SCOLARO L, GRACEFFA G, DI GAUDIO F, and BAZAN V AND RUSSO A

Subjects
hereditary breast/ovarian cancer
Published
2007

23. TP53, Ki-Ras and P16INK4A gene molecular analysis in salivary gland tumors

Author

FIORENTINO, Francesco Paolo, AGNESE, Valentina, CALÒ V, BRUNO L, AUGELLO, Claudia, CASCIO, Sandra, BARBERA, Floriana, GARGANO G, GREGORIO, Valter, CALCARA, Donatella, LA PAGLIA L, SCHIRO', Valentina, TERRASI, Marianna, FANALE, Daniele, SCOLARO, Laura, GRACEFFA, Giuseppa, DI GAUDIO, Francesca, RUSSO, Antonio, FERLA, Rita, BAZAN, Viviana, FIORENTINO F, AGNESE V, CALÒ V, BRUNO L, AUGELLO C, CASCIO S, BARBERA F, GARGANO G, GREGORIO V, CALCARA D, LA PAGLIA L, SCHIRO V, TERRASI M, FANALE D, SCOLARO L, GRACEFFA G, DI GAUDIO F, RUSSO A, Ferla, R., and Bazan, V.

Published
2007

24. Aberrant methylation within RUNX3 CpG island associated with the nuclear and mitochondrial microsatellite instability in sporadic gastric cancers. Results of a GOIM (Gruppo Oncologico dell'Italia Meridionale) prospective study

Author

Antonio Russo, C. Intrivici, Fabio Fulfaro, Corsale S, Giuseppe Colucci, Gianni Pantuso, Laura Ottini, V. Bazan, Vincenza Morello, Valentina Agnese, Donatella Calcara, Massimo Cajozzo, Rosa Maria Tomasino, Gargano G, GARGANO, G, CALCARA, D, CORSALE, S, AGNESE, V, INTRIVICI, C, FULFARO, F, PANTUSO, G, CAJOZZO, M, MORELLO, V, TOMASINO, RM, OTTINI, L, COLUCCI, G, BAZAN, V, and RUSSO, A

Subjects
Male, Mitochondrial DNA, GC Rich Sequence, Biology, DNA, Mitochondrial, law.invention, law, Stomach Neoplasms, medicine, Humans, Genetic Predisposition to Disease, Prospective Studies, Polymerase chain reaction, Aged, Cell Nucleus, Cancer, Microsatellite instability, Hematology, Methylation, DNA Methylation, Middle Aged, medicine.disease, Molecular biology, digestive system diseases, Core Binding Factor Alpha 3 Subunit, Oncology, CpG site, Microsatellite, CpG Islands, Female, Microsatellite Instability, Microsatellite Repeats
Abstract

Background: Gastric cancer (GC) development is a multistep process, during which numerous alterations accumulate in nuclear and mitochondrial DNA. A deficiency of repair machinery brings about an accumulation of errors introduced within simple repetitive microsatellite sequences during replication of DNA. Aberrant methylation is related to microsatellite instability (MSI) by the silencing of the hMLH1 gene. The aim of this study is to investigate a possible relationship between the RUNX3 promoter methylation, nuclear microsatellite instability (nMSI) and mitochondrial microsatellite instability (mtMSI), in order to clarify its biological role in GC. Patients and methods: nMSI and mtMSI were evaluated in a consecutive series of 100 GC patients. For the analysis of the nMSI, we followed the National Cancer Institute guidelines. mtMSI was assessed by analyzing a portion of the displacement-loop region. The aberrant methylation of RUNX3 was analyzed in 40 GC patients by methylation-specific PCR. Results: Overall, 55% of GC demonstrated methylation of the RUNX3 promoter; 82% of GC was classified as stable microsatellite instability, 5% as low-level microsatellite instability and 13% as high-level microsatellite instability (MSI-H); mtMSI was detected in 11 % of GC. A significant association was found between mtMSI and tumor-node-metastasis staging, furthermore an interesting association between MSI-H status, mtMSI and RUNX3 methylation. Conclusion: These data suggest that RUNX3 is an important target of methylation in the evolution of mtMSI and nMSI-H GC.

Published
2007

25. Aurora-A overexpression as an early marker of reflux-related columnar mucosa and Barrett's oesophagus

Author

C. Intrivici, Nicola Gebbia, F Aragona, Antonio Russo, Valentina Agnese, Daniela Cabibi, Gianni Pantuso, Eugenio Fiorentino, Donatella Calcara, Marianna Terrasi, V. Bazan, Giuseppe Colucci, AGNESE, V, CABIBI, D, CALCARA, D, TERRASI, M, PANTUSO, G, FIORENTINO, E, INTRIVICI, C, COLUCCI, G, ARAGONA, F, GEBBIA, N, BAZAN, V, and RUSSO, A

Subjects
Adult, Male, medicine.medical_specialty, Pathology, Esophageal Neoplasms, Settore MED/06 - Oncologia Medica, Aneuploidy, Settore BIO/11 - Biologia Molecolare, Adenocarcinoma, Protein Serine-Threonine Kinases, Settore MED/08 - Anatomia Patologica, Gastroenterology, Barrett Esophagus, Aurora Kinases, Internal medicine, Biopsy, medicine, Humans, Aurora-A overexpression, Barrett’s oesophagus, cell cycle, columnar-lined oesophagus, p53 protein, Prospective Studies, Esophagus, Mucous Membrane, medicine.diagnostic_test, Esophageal disease, business.industry, Intestinal metaplasia, Hematology, Middle Aged, medicine.disease, medicine.anatomical_structure, Oncology, Dysplasia, Barrett's esophagus, Gastroesophageal Reflux, Female, business, Immunostaining, Biomarkers
Abstract

BACKGROUND: The development of oesophageal adenocarcinoma is generally closely associated with the presence of a specialised intestinal-type epithelium such as that found in Barrett's oesophagus (BO). A particular histological condition is when the distal oesophagus showing cardiac and/or fundic mucosa without intestinal metaplasia cannot be defined as 'Barrett's mucosa' [condition that we call 'columnar-lined oesophagus' (CLO)] and up till now, there has been no agreement in literature about the management of this condition. Aurora-A overexpression leads to centrosome amplification, chromosomal instability and aneuploidy in mammalian cells. PATIENTS AND METHODS: A prospective study was carried out on 28 consecutive patients who presented columnar mucosa above the gastro-oesophageal junction (GOJ) at endoscopy. As controls, two more biopsies were obtained, one on the normal-appearing squamous oesophagus above the GOJ, as far as possible from the columnar mucosa (controls A), and one taken 1 cm below the GOJ (controls B). The Aurora-A and p53 expression levels were analysed respectively by Quantitative Real Time PCR and immunohistochemistry. RESULTS: Twelve patients were affected by BO (43%) while the other 16 patients (57%) had a CLO. Nine of 28 (32%) cases were focally positive for p53 immunostaining. All the BO/CLO samples were positive for the Aurora-A transcript with regard to controls. Furthermore, 13 of 28 (46%) cases showed overexpression (above the median for the whole group). CONCLUSION: Due to the low number of cases, we are not at present able to state that statistically significant quantitative differences in Aurora-A messenger RNA expression exist between CLO and BO cases with and without dysplasia and p53-positive immunostaining. Further studies on a larger number of cases with a follow-up period are necessary in order to establish the risk of progression and the correct management of these subjects.

Published
2007

26. Molecular analysis of TP53, Ki-Ras and P16 methylation status in tissue and plasma of subjects affected by gastrointestinal cancer (GIC)

Author

SCOLARO, Laura, AGNESE, Valentina, CAL V, BRUNO L, AUGELLO, Claudia, CASCIO, Sandra, GARGANO G, BARBERA, Floriana, GREGORIO, Valter, CALCARA, Donatella, LA PAGLIA L, SCHIRO', Valentina, TERRASI, Marianna, FANALE, Daniele, FERLA, Rita, FIORENTINO, Francesco Paolo, FODDAI, Elena, DI GAUDIO, Francesca, GRACEFFA, Giuseppa, BAZAN, Viviana, RUSSO, Antonio, SCOLARO L, AGNESE V, CAL V, BRUNO L, AUGELLO C, CASCIO S, GARGANO G, BARBERA F, GREGORIO V, CALCARA D, LA PAGLIA L, SCHIRO V, TERRASI M, FANALE D, FERLA R, FIORENTINO FP, FODDAI E, DI GAUDIO F, GRACEFFA G, BAZAN, V, and RUSSO, A

Subjects
Settore MED/06 - Oncologia Medica, Settore MED/08 - Anatomia Patologica, Molecular analysis TP53, GIC
Abstract

BACKGROUND: Despite the improvement in detection and surgical therapy in the last years, the outcome of patients affected by colorectal carcinoma (CRC) remains limited by metastatic relapse. The aim of this study was to investigate the presence of free tumor DNA in the plasma of CRC patients in order to understand its possible prognostic role. PATIENTS AND METHODS: Ki-Ras, TP53 mutations and p16(INK4A) methylation status were prospectively evaluated in tumor tissues and plasma of 66 CRC patients. RESULTS: In 50 of the 66 primitive tumor cases (76%) at least one significant alteration was identified in Ki-Ras and/or TP53 and/or p16(INK4A) genes. Eighteen of the 50 patients presented the same alteration both in the plasma and in the tumor tissue. At univariate analysis, Ki-Ras mutations proved to be significantly related to quicker relapse (P

Published
2007

27. Expression level of the mammaglobin (MGB1) gene in BC:possibile index of BC progression

Author

CALCARA, Donatella, AGNESE, Valentina, BARBERA, Floriana, CASCIO, Sandra, SCHIRO', Valentina, TERRASI, Marianna, FANALE, Daniele, FERLA, Rita, FIORENTINO, Francesco Paolo, SCOLARO, Laura, BAZAN, Viviana, RUSSO, Antonio, GARGANO G, CAL V, BRUNO L, LA PAGLIA L, CALCARA D, GARGANO G, AGNESE V, BARBERA F, CAL V, BRUNO L, CASCIO S, SCHIRO' V, TERRASI M, LA PAGLIA L, FANALE D, FERLA R, FIORENTINO FP, SCOLARO L, BAZAN, V, and RUSSO A

Published
2007

28. A study of a new germline mutation in BRCA1 gene in two Sicilian families: a founder mutation?

Author

LA PAGLIA L, CAL V, CORSALE S, GARGANO G, ADAMO B, AGNESE, Valentina, BRUNO, Loredana, AUGELLO, Claudia, GREGORIO, Valter, CASCIO, Sandra, GULLO, Arianna, BARBERA, Floriana, TERRASI, Marianna, SCHIRO', Valentina, CALCARA, Donatella, MORELLO, Vincenza, TOMASINO, Rosa Maria, BAZAN, Viviana, RUSSO, Antonio, CALO', Valentina, LA PAGLIA L, CAL V, AGNESE V, CORSALE S, BRUNO L, AUGELLO C, GREGORIO V, CASCIO S, GULLO A, GARGANO G, BARBERA F, TERRASI M, SCHIRO V, CALCARA D, ADAMO B, MORELLO V, TOMASINO RM, BAZAN V, RUSSO A, and Calo', V.

Published
2006

29. A missense mutation associated to early onset breast cancer in a sicilian woman

Author

BARBERA, Floriana, AGNESE, Valentina, BRUNO, Loredana, AUGELLO, Claudia, GREGORIO, Valter, CASCIO, Sandra, GULLO, Arianna, TERRASI, Marianna, SCHIRO', Valentina, CALCARA, Donatella, MORELLO, Vincenza, TOMASINO, Rosa Maria, BAZAN, Viviana, RUSSO, Antonio, CALO', Valentina, GARGANO G, CAL V, CORSALE S, BARBERA F, LA PAGLIA L, ADAMO B, BARBERA F, GARGANO G, CAL V, AGNESE V, CORSALE S, BRUNO L, AUGELLO C, GREGORIO V, CASCIO S, GULLO A, LA PAGLIA L, TERRASI M, SCHIRO V, CALCARA D, ADAMO B, MORELLO V, TOMASINO RM, BAZAN V, RUSSO A, and Calo', V.

Published
2006

30. TP53 mutations and microsatellite instability are prognostic factors in gastric cancer?

Author

CORSALE S, BRUNO L, GARGANO G, LA PAGLIA L, RINALDI G, TOMASINO R. M, MARTORANA A, AGNESE, Valentina, AUGELLO, Claudia, CALCARA, Donatella, CALO', Valentina, GREGORIO, Valter, GULLO, Arianna, SCHIRO', Valentina, TERRASI, Marianna, CROSTA, Adele, MORELLO, Vincenza, DANIELE, Elio, PANTUSO, Gianni, FIORENTINO, Eugenio, CHIARINI, Alfredo, BAZAN, Viviana, RUSSO, Antonio, CORSALE S, AGNESE V, AUGELLO C, BRUNO L, CALCARA D, CALò, V, GARGANO G, GREGORIO V, GULLO A, LA PAGLIA L, SCHIRò, V, TERRASI M, CROSTA A, RINALDI G, TOMASINO R M, MORELLO V, DANIELE E, PANTUSO G, FIORENTINO E, CHIARINI A, MARTORANA A, BAZAN V, and RUSSO A

Published
2006

31. Significance of P16INK4A hypermethylation gene in primary head/neck and colorectal tumors: it is a specific tissue event? Results of a 3-year GOIM (Gruppo Oncologico dell'Italia Meridionale) prospective study

Author

Rosa Maria Tomasino, V. Adamo, Antonio Russo, Federica Latteri, Maria Rosaria Valerio, Gaetana Rinaldi, Mario Adelfio Latteri, Donatella Calcara, Loredana Bruno, Vincenza Morello, Vito Rodolico, G. Di Fede, Nello Grassi, Giuseppe Altavilla, Eugenio Fiorentino, Viviana Bazan, Valentina Agnese, Antonino Agrusa, Adele Crosta, Giuseppe Cicero, Corsale S, Giuseppe Colucci, Claudia Augello, Valentina Calò, AGNESE V, CORSALE S, CALO V, AUGELLO C, BRUNO L, CALCARA D, CROSTA A, RODOLICO V, RINALDI G, CICERO G, LATTERI F, AGRUSA A, MORELLO V, ADAMO V, ALTAVILLA G, DI FEDE G, FIORENTINO E, GRASSI N, LATTERI M, VALERIO MR, TOMASINO RM, COLUCCI G, BAZAN V, and RUSSO A

Subjects
Oncology, medicine.medical_specialty, Colorectal cancer, Internal medicine, medicine, Humans, Promoter Regions, Genetic, Prospective cohort study, Neoplasm Staging, Univariate analysis, business.industry, Genes, p16, Incidence (epidemiology), Cancer, Hematology, Methylation, DNA Methylation, medicine.disease, Head and Neck Neoplasms, Salivary gland cancer, DNA methylation, Carcinoma, Squamous Cell, Colorectal Neoplasms, business, P16INK4A, head and neck carcinoma
Abstract

Background Methylation of the p16 promoter is one of the most frequent mechanisms of gene inactivation; its incidence is extremely variable according to the type of tumor involved. Our purpose was to analyze the hypermethylation of the p16 promoter in laryngeal squamous cell carcinomas (LSCC), salivary gland (SG) tumors and in colorectal cancer (CRC), to detect any possible association with the clinicopathological features and to determine the prognostic significance of the p16 gene in the tumors analyzed. Patients and methods The hypermethylation of the p16 promoter was prospectively analyzed, by MSP, in a consecutive series of 64 locally advanced LSCC patients, in a consecutive series of 33 SG tumor patients and in a consecutive series of 66 sporadic CRC patients. Results Hypermethylation was observed in 9% of the LSCC cases, in all cases of SG cancer and in 21% of the CRC cases. No significant association was observed between p16 hypermethylation and clinicopathological variables in all the tissue samples analyzed. Moreover at univariate analysis p16 mutations were not independently related at disease relapse and death in LSCC and CRC. Conclusions The results of this study suggest that the lack of p16 function could happen in advanced stage of SG tumors.

Published
2006

32. Ductal Lavage: a valid method of risk assessment and of early diagnosis in breast cance

Author

CALCARA, Donatella, GREGORIO, Valter, AGNESE, Valentina, AUGELLO, Claudia, SCHIRO', Valentina, TERRASI, Marianna, CROSTA, Adele, BADALAMENTI, Giuseppe, NAPOLI, Luisa, MORELLO, Vincenza, DANIELE, Elio, CHIARINI, Alfredo, GEBBIA, Nicolo', CORSALE S, CAL V, BRUNO L, GARGANO G, GULLO A, LA PAGLIA L, FRICANO S, GIORDANO A, TOMASINO RM, MARTORANA A, BAZAN V. AND RUSSO A., CALCARA D, GREGORIO V, CORSALE S, CAL V, AGNESE V, AUGELLO C, BRUNO L, GARGANO G, GULLO A, LA PAGLIA L, SCHIRO' V, TERRASI M, CROSTA A, BADALAMENTI G, FRICANO S, NAPOLI L, GIORDANO A, TOMASINO RM, MORELLO V, DANIELE E, CHIARINI A, MARTORANA A, GEBBIA N, and BAZAN V AND RUSSO A

Published
2006

34. BRCA1 germline mutations in Sicilian breast and/or ovarian cancer families and their implications for genetic counselling

Author

CAL V, CORSALE S, GARGANO G, LA PAGLIA L, ADAMO B, AGNESE, Valentina, BRUNO, Loredana, AUGELLO, Claudia, GREGORIO, Valter, CASCIO, Sandra, GULLO, Arianna, BARBERA, Floriana, TERRASI, Marianna, SCHIRO', Valentina, CALCARA, Donatella, MORELLO, Vincenza, TOMASINO, Rosa Maria, BAZAN, Viviana, CALO', Valentina, RUSSO, Antonio, CAL V, AGNESE V, CORSALE S, BRUNO L, AUGELLO C, GREGORIO V, CASCIO S, GULLO A, GARGANO G, BARBERA F, LA PAGLIA L, TERRASI M, SCHIRO V, CALCARA D, ADAMO B, MORELLO V, TOMASINO RM, BAZAN V, Calo', V., and Russo, A.

Published
2006

35. The revised Approved Instructional Resources score: An improved quality evaluation tool for online educational resources.

Author

Grock A, Jordan J, Zaver F, Colmers-Gray IN, Krishnan K, Chan T, Thoma B, Alexander C, Alkhalifah M, Almehlisi AS, Alqahtani S, Anderson S, Anderson S, Andrews C, Andruko J, Antony N, Aryal D, Backus B, Baird J, Baker A, Batty S, Baylis J, Beaumont B, Belcher C, Benavides B, Benham M, Botta J, Berger Pelletier E, Bouchard N, Brazil V, Brumfield E, Bryson A, Bunchit W, Butler K, Buzikievich L, Calcara D, Carey R, Carrillo MR, Carroll S, Lyons C, Cassidy L, Challen K, Chan K, Chaplin T, Chatham-Zvelebil N, Chen E, Chen L, Chhabra S, Chin A, Chochi E, Choudhri T, Christensen J, Connors K, Coppersmith V, Cosgrove A, Costello G, Cullison K, D'Alessandro A, Wit K, Decock M, Delbani R, Denq W, Deutscher J, Devine B, Dorsett M, Duda T, Dueweke J, Dunphy T, Dyer S, Eastley KT, Edmonds M, Edwards K, Ehrman R, Elkhalidy Y, Fedor P, Ficiur B, Flynn C, Fraser B, Fu M, Fukakusa J, Funk E, Gaco D, Gawlik V, Ghaffarian K, Gharahbaghian L, Griffith A, Griffith P, Gronowski T, Grossman C, Gucwa J, Gupta P, Gustafson A, Guy A, Haas M, Haciski S, Hajdinjak E, Hall AK, Hammock R, Hansel J, Hart A, Hattin L, Herb B, Hilbert S, Hill J, Hill J, Ho A, House E, House N, Huang SYM, Huffman J, Inboriboon C, Ireland A, Jamal A, Jamil MA, Jansen V, Jarou Z, Jia V, Johnston L, Kalnow D, Kapur P, Kelly S, Kelson K, Kent W, Khakhkhar R, Khurana J, Kilp A, Knapp S, Kohler S, Kruhlak I, Lalani N, Lam S, Lank P, Laurie Z, Lea K, Leber E, Lee CH, Lenes H, Lenora N, Leontowicz J, Lien K, Lin M, Lin Y, Little A, Liu H, Liu I, Liu S, Louka S, Lovell E, Lowe D, Lubberdink A, Luc J, Ma SH, MacLeod H, Mancuso N, Maneshi A, May J, Mayo J, McDonnell M, McLellan S, McQuarrie C, Mead T, Meeuwisse C, Meloy P, Menzies P, Messman A, Miazga S, Mills L, Mix A, Montag S, Moore B, Morgenstern J, Mott S, Mukherj P, Mulla A, Nandalal S, Nikel T, Nood J, Nugent S, Oakland M, Oberholzer W, Otugo O, Oyedokun TS, Pardhan A, Patel K, Paterson Q, Patocka C, Patterson C, Pearlman J, Pelletier-Bui A, Phan M, Poonja Z, Powell A, Premkumar K, Prosen G, Puri V, Quaife T, Raffel R, Raja A, Ramunno R, Rang L, Rannazzisi S, Regan S, Rezaie SR, Ridderikhof M, Rogers V, Roh C, Rosenberg K, Roure M, Rudinsky S, Rudner J, Saleh A, Sanderson W, Scheirer O, Schofield P, Schunk P, Schwarz E, Shahrabadi P, Shappell E, Sheffield J, Singh M, Singson HC, Slessor D, Smith S, Sneath P, Sobehart R, Spearing K, Stempien J, Sternard B, Stratton T, Stuart K, Stuntz B, Susalla M, Sweeney C, Swisher L, Swoboda H, Syed S, Taira T, Tambe N, Tang R, Targonsky E, Taylor A, Taylor R, Taylor T, Ting P, Tiwald G, Tran E, Tran K, Trickovic J, Trinquero P, Tyagi A, Umana M, Vallance P, Van den Berg P, van Diepen K, Vargas L, Verbeek R, Viggers S, Vlodaver Z, Wagner M, Walji N, Walter J, Wan M, Wang R, Wanner G, Warawa W, Ward M, Weekes J, Weersink K, Weessies C, Whalen-Browne A, Whiteside B, Willis M, Wilmer J, Wong N, Woodcroft M, Woods R, Yau L, Yee J, Yeh C, Yurkiw K, Zaver F, and Zozula A

Abstract

Background: Free Open-Access Medical education (FOAM) use among residents continues to rise. However, it often lacks quality assurance processes and residents receive little guidance on quality assessment. The Academic Life in Emergency Medicine Approved Instructional Resources tool (AAT) was created for FOAM appraisal by and for expert educators and has demonstrated validity in this context. It has yet to be evaluated in other populations., Objectives: We assessed the AAT's usability in a diverse population of practicing emergency medicine (EM) physicians, residents, and medical students; solicited feedback; and developed a revised tool., Methods: As part of the Medical Education Translational Resources: Impact and Quality (METRIQ) study, we recruited medical students, EM residents, and EM attendings to evaluate five FOAM posts with the AAT and provide quantitative and qualitative feedback via an online survey. Two independent analysts performed a qualitative thematic analysis with discrepancies resolved through discussion and negotiated consensus. This analysis informed development of an initial revised AAT, which was then further refined after pilot testing among the author group. The final tool was reassessed for reliability., Results: Of 330 recruited international participants, 309 completed all ratings. The Best Evidence in Emergency Medicine (BEEM) score was the component most frequently reported as difficult to use. Several themes emerged from the qualitative analysis: for ease of use-understandable, logically structured, concise, and aligned with educational value. Limitations include deviation from questionnaire best practices, validity concerns, and challenges assessing evidence-based medicine. Themes supporting its use include evaluative utility and usability. The author group pilot tested the initial revised AAT, revealing a total score average measure intraclass correlation coefficient (ICC) of moderate reliability (ICC = 0.68, 95% confidence interval [CI] = 0 to 0.962). The final AAT's average measure ICC was 0.88 (95% CI = 0.77 to 0.95)., Conclusions: We developed the final revised AAT from usability feedback. The new score has significantly increased usability, but will need to be reassessed for reliability in a broad population., Competing Interests: The authors have no potential conflicts to disclose. All authors had full access to all the study data and had final responsibility for the decision to submit for publication., (© 2021 by the Society for Academic Emergency Medicine.)

Published
2021
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36. Analysis of globule types in malignant melanoma.

Author

Xu J, Gupta K, Stoecker WV, Krishnamurthy Y, Rabinovitz HS, Bangert A, Calcara D, Oliviero M, Malters JM, Drugge R, Stanley RJ, Moss RH, and Celebi ME

Subjects
Cohort Studies, Diagnosis, Differential, False Negative Reactions, False Positive Reactions, Female, Humans, Male, Melanoma classification, Nevus, Pigmented classification, Odds Ratio, Predictive Value of Tests, Probability, Retrospective Studies, Sensitivity and Specificity, Skin Neoplasms classification, Dermoscopy methods, Melanoma pathology, Nevus, Pigmented pathology, Skin Neoplasms pathology
Abstract

Objective: To identify and analyze subtypes of globules based on size, shape, network connectedness, pigmentation, and distribution to determine which globule types and globule distributions are most frequently associated with a diagnosis of malignant melanoma., Design: Retrospective case series of dermoscopy images with globules., Setting: Private dermatology practices., Participants: Patients in dermatology practices. Intervention Observation only. Main Outcome Measure Association of globule types with malignant melanoma., Results: The presence of large globules (odds ratio [OR], 5.25) and globules varying in size (4.72) or shape (5.37) had the highest ORs for malignant melanoma among all globule types and combinations studied. Classical globules (dark, discrete, convex, and 0.10-0.20 mm) had a higher risk (OR, 4.20) than irregularly shaped globules (dark, discrete, and not generally convex) (2.89). Globules connected to other structures were not significant in the diagnosis of malignant melanoma. Of the different configurations studied, asymmetric clusters have the highest risk (OR, 3.02)., Conclusions: The presence of globules of varying size or shape seems to be more associated with a diagnosis of malignant melanoma than any other globule type or distribution in this study. Large globules are of particular importance in the diagnosis of malignant melanoma.

Published
2009
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