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2 results on '"CYTK-1 GENE"'

1. Rapid discrimination of cytK-1 and cytK-2 genes in Bacillus cereus strains by a novel duplex PCR system.

Author

Guinebretiere, Marie-Hélène, Fagerlund, Annette, Granum, Per E., and Nguyen-The, Christophe

Subjects
*BACILLUS cereus, *POLYMERASE chain reaction, *GENES, *DIARRHEA, *ENTEROTOXINS
Abstract

Bacillus cereus is the causative agent of gastrointestinal diarrhoea. At least three known enterotoxins may be involved in this syndrome: nonhaemolytic (Nhe) enterotoxin, Hbl enterotoxin and cytotoxin K. Two different forms were recently described for cytotoxin K, encoded by cytK-1 and cytK-2 genes. The CytK-1 toxin appeared to carry a high toxicity, but there is currently no method available to rapidly detect and discriminate the B. cereus strains able to produce this CytK-1 form. In this study, a duplex PCR assay was developed and validated on 162 known cytotoxin-containing strains. This PCR method is the first molecular tool to provide rapid detection and discrimination of cytK-1- and cytK-2-carrying B. cereus strains. [ABSTRACT FROM AUTHOR]

Published
2006
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2. Rapid discrimination of cytK-1 and cytK-2 genes in Bacillus cereus strains by a novel duplex PCR system

Author

Marie-Hélène Guinebretiere, Christophe Nguyen-The, Per Einar Granum, Annette Fagerlund, Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Department of Food Safety and Infection Biology, and Norwegian School of Veterinary Science

Subjects
Time Factors, Bacterial Toxins, Molecular Sequence Data, Bacillus cereus, BACILLUS CEREUS, Enterotoxin, Biology, medicine.disease_cause, CYTOTOXIN K, Polymerase Chain Reaction, Microbiology, Rapid detection, CYTK-2 GENE, law.invention, DETECTION, 03 medical and health sciences, law, Genetics, medicine, DIARRHOEAL FOOD POISONING, Humans, CYTK-1 GENE, Molecular Biology, Gene, Polymerase chain reaction, DNA Primers, 030304 developmental biology, 0303 health sciences, Base Sequence, 030306 microbiology, Toxin, biology.organism_classification, Molecular biology, 3. Good health, Duplex pcr, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Cereus, Genes, Bacterial, bacteria
Abstract

International audience; Bacillus cereus is the causative agent of gastrointestinal diarrhoea. At least three known enterotoxins may be involved in this syndrome: nonhaemolytic (Nhe) enterotoxin, Hbl enterotoxin and cytotoxin K. Two different forms were recently described for cytotoxin K, encoded by cytK-1 and cytK-2 genes. The CytK-1 toxin appeared to carry a high toxicity, but there is currently no method available to rapidly detect and discriminate the B. cereus strains able to produce this CytK-1 form. In this study, a duplex PCR assay was developed and validated on 162 known cytotoxin-containing strains. This PCR method is the first molecular tool to provide rapid detection and discrimination of cytK-1- and cytK-2-carrying B. cereus strains.

Published
2006

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