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Rapid discrimination of cytK-1 and cytK-2 genes in Bacillus cereus strains by a novel duplex PCR system
- Source :
- FEMS Microbiology Letters, FEMS Microbiology Letters, Wiley-Blackwell, 2006, 259 (1), pp.74-80. ⟨10.1111/j.1574-6968.2006.00247.x⟩
- Publication Year :
- 2006
- Publisher :
- HAL CCSD, 2006.
-
Abstract
- International audience; Bacillus cereus is the causative agent of gastrointestinal diarrhoea. At least three known enterotoxins may be involved in this syndrome: nonhaemolytic (Nhe) enterotoxin, Hbl enterotoxin and cytotoxin K. Two different forms were recently described for cytotoxin K, encoded by cytK-1 and cytK-2 genes. The CytK-1 toxin appeared to carry a high toxicity, but there is currently no method available to rapidly detect and discriminate the B. cereus strains able to produce this CytK-1 form. In this study, a duplex PCR assay was developed and validated on 162 known cytotoxin-containing strains. This PCR method is the first molecular tool to provide rapid detection and discrimination of cytK-1- and cytK-2-carrying B. cereus strains.
- Subjects :
- Time Factors
Bacterial Toxins
Molecular Sequence Data
Bacillus cereus
BACILLUS CEREUS
Enterotoxin
Biology
medicine.disease_cause
CYTOTOXIN K
Polymerase Chain Reaction
Microbiology
Rapid detection
CYTK-2 GENE
law.invention
DETECTION
03 medical and health sciences
law
Genetics
medicine
DIARRHOEAL FOOD POISONING
Humans
CYTK-1 GENE
Molecular Biology
Gene
Polymerase chain reaction
DNA Primers
030304 developmental biology
0303 health sciences
Base Sequence
030306 microbiology
Toxin
biology.organism_classification
Molecular biology
3. Good health
Duplex pcr
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Cereus
Genes, Bacterial
bacteria
Subjects
Details
- Language :
- English
- ISSN :
- 03781097 and 15746968
- Database :
- OpenAIRE
- Journal :
- FEMS Microbiology Letters, FEMS Microbiology Letters, Wiley-Blackwell, 2006, 259 (1), pp.74-80. ⟨10.1111/j.1574-6968.2006.00247.x⟩
- Accession number :
- edsair.doi.dedup.....61f7f853ba5b5d4b98551b231c4e7717