1. A systems biology view of wood formation in Eucalyptus grandis trees submitted to different potassium and water regimes
- Author
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Mônica T. Veneziano Labate, Fabien Mounet, Marie Morel, Jacqueline Grima-Pettenati, Marie Denis, Raphael Ployet, Mathias Christina, Jean-Paul Laclau, Gilles Chaix, Thais Regiani Cataldi, Carlos Alberto Labate, Mario Tomazello Filho, Bénédicte Favreau, Hélène San Clemente, Laboratoire de Recherche en Sciences Végétales (LRSV), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Interactions Sol Plante Atmosphère (ISPA), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Auteur indépendant, Aide à la Décision pour une Médecine Personnalisé - Laboratoire de Biostatistique, Epidémiologie et Recherche Clinique - EA 2415 (AIDMP), Université de Montpellier (UM)-Université Montpellier 1 (UM1), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), University of São Paulo, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Interactions Sol Plante Atmosphère (UMR ISPA), Université Montpellier 1 (UM1)-Université de Montpellier (UM), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Régulation et Dynamique de la Formation du Bois, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Ecofor, Allenvi, French National Research Infrastructure ANAEE-F, Centre National pour la Recherche Scientifique (CNRS), French Laboratory of Excellence project 'TULIP' : ANR-10-LABX-41, ANR-11-IDEX-0002-02, Agropolis Fondation : 1203-003, ANR-10-LABX-372 0001-01, CAPES : 017/13, CNPq : 444793/2014-3, 014/2012, FAPESP : 2013/25642-5, Ministere de l'Education Nationale, de l'Enseignement Superieur et de la Recherche, ANR-11-IDEX-0002,UNITI,Université Fédérale de Toulouse(2011), and ANR-10-LABX-0001,AGRO,Agricultural Sciences for sustainable Development(2010)
- Subjects
0106 biological sciences ,0301 basic medicine ,K50 - Technologie des produits forestiers ,SECA ,Physiology ,tree physiology ,Gene regulatory network ,Biomass ,Plant Science ,drought ,01 natural sciences ,Trees ,transcriptomics ,[SDV.SA.SF]Life Sciences [q-bio]/Agricultural sciences/Silviculture, forestry ,Cell Wall ,Gene Regulatory Networks ,Plant Proteins ,Eucalyptus ,Wood production ,wood traits ,Systems Biology ,system biology ,potassium ,metabolomics ,Phenotype ,Propriété technologique ,Metabolome ,Besoin en eau ,gene network ,Secondary cell wall ,secondary cell wall ,wood ,Eucalyptus grandis ,abiotic stress ,F60 - Physiologie et biochimie végétale ,Biology ,03 medical and health sciences ,Bois ,Metabolomics ,Xylem ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Botany ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Abiotic stress ,Water ,15. Life on land ,saccharification ,030104 developmental biology ,Transcriptome ,Transcription Factors ,010606 plant biology & botany ,F04 - Fertilisation - Abstract
International audience; Wood production in fast-growing Eucalyptus grandis trees is highly dependent on both potassium (K) fertilization and water availability but the molecular processes underlying wood formation in response to the combined effects of these two limiting factors remain unknown. E. grandis trees were submitted to four combinations of K-fertilization and water supply. Weighted gene co-expression network analysis and MixOmics-based co-regulation networks were used to integrate xylem transcriptome, metabolome and complex wood traits. Func- tional characterization of a candidate gene was performed in transgenic E. grandis hairy roots. This integrated network-based approach enabled us to identify meaningful biological pro- cesses and regulators impacted by K-fertilization and/or water limitation. It revealed that modules of co-regulated genes and metabolites strongly correlated to wood complex traits are in the heart of a complex trade-off between biomass production and stress responses. Nested in these modules, potential new cell-wall regulators were identified, as further con- firmed by the functional characterization of EgMYB137. These findings provide new insights into the regulatory mechanisms of wood formation under stressful conditions, pointing out both known and new regulators co-opted by K-fertilization and/or water limitation that may potentially promote adaptive wood traits.
- Published
- 2019
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