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Introduction Photoaging is the process by which ultraviolet rays gradually induce clinical and histological changes in the skin through the production and organization of biological molecules, such as elastin, which is critical to skin strength and elasticity. After exposure to radiation, elastin may undergo alternative mRNA splicing, resulting in modified proteins that contribute to the formation of aging characteristics, such as solar elastosis. The present work aimed to study two different forms of elastin under these conditions: normal elastin and elastin that had been altered in exon 26A. Methods These different forms of elastin were characterized for gene expression by quantitative real-time polymerase chain reaction (qPCR) and for protein expression by immunohistochemistry of ex vivo skins (from photoexposed and non-photoexposed areas) and in vitro reconstituted skin. In addition, up- and downstream molecules in the elastin signaling cascade were evaluated. Results As a result, a significant increase in the gene expression of elastin 26A was observed in both ex vivo photoexposed skin tissues and the in vitro photoexposed reconstituted skins. Additionally, significant increases in the gene expression levels of matrix metalloproteinase-12 (MMP12) and lysyl oxidase (LOX) were observed in the ex vivo skin model. The evaluation of protein expression levels of some photoaging markers on the reconstituted skin revealed increased tropoelastin and fibrillin-1 expression after photoexposure. Conclusion This work contributes to a better understanding of the biological mechanisms involved in photoaging, making it possible to obtain new strategies for the development of dermocosmetic active ingredients to prevent and treat skin aging.

Merit is a central pillar of liberal epistemology, humanism, and democracy. The scientific enterprise, built on merit, has proven effective in generating scientific and technological advances, reducing suffering, narrowing social gaps, and improving the quality of life globally. This perspective documents the ongoing attempts to undermine the core principles of liberal epistemology and to replace merit with non-scientific, politically motivated criteria. We explain the philosophical origins of this conflict, document the intrusion of ideology into our scientific institutions, discuss the perils of abandoning merit, and offer an alternative, human-centered approach to address existing social inequalities.

Injectable scaffolds are a promising strategy to restore and regenerate damaged and diseased tissues. They require minimally invasive procedure and allow the formation of an in-situ structure of any shape. However, the formation of 3D in-situ structure with aligned morphologies using a method which could be easily transferred to clinical settings remains a challenge. Herein, the rational design of an aligned injectable hydrogel-based scaffold via remote-induced alignment is reported. Carboxylated multi-walled carbon nanotubes (cMWCNT) are aligned into hydrogel via low magnetic field. The uniform dispersion and alignment of cMWCNT into the hydrogel are clearly demonstrated by small angle neutron scattering. The obtained aligned cMWCNT-embodied hydrogel is stable over 7 days at room temperature and as well at body temperature (i.e. 37 °C). As unique approach, the formation of MWCNT-hydrogel composite is investigated combining rheology with molecular dynamic and quantum mechanical calculations. The increase of MWCNT concentration into the hydrogel decreases the total energy promoting structural stabilization and increase of stiffness. The remote aligning of injectable hydrogel-based scaffold opens up horizons in the engineering of functional tissues which requires specific cell orientation. publishedVersion

Este artigo tem como objetivo contribuir para uma melhor compreens?o da import?ncia do marketing relacional na gest?o de um restaurante. Para o efeito, desenvolve-se um estudo de caso de um conceituado restaurante localizado na cidade de Barcelona ? o restaurante Sete Portes. Recorrendo ao Modelo dos seis mercados de Christopher, Payne e Ballantyne (2002), ilustra-se o modelo de gest?o de rela??es deste restaurante e demonstra-se a sua relev?ncia no processo de cria??o valor para os diferentes stakeholders. Este estudo de caso poder? ser uma ferramenta ?til no processo de ensino do marketing relacional em cursos de licenciatura e mestrado. 8316-FD77-85EB | Pedro Miguel Fonseca Moreira de Carvalho info:eu-repo/semantics/publishedVersion

The cosmetic industry's growing concern about the impact of its supply chain on the environment, sustainability of raw materials, and biodiversity increases the need to ensure that the final product has a lower environmental impact. The objective of this review is to summarize and compare the information available from international organizations and legislation regarding the main criteria used to assess raw materials for aquatic toxicity, as well as the most suitable alternative methods for obtaining assessment parameters. Using the literature available in databases, a review of the scientific literature and international legislation, this work discusses and compares the parameters established by international organizations such as the Environmental Protection Agency (EPA) and Cradle to Cradle (C2C), as well as European legislation, namely, European Regulation 1272/2008, for assessing environmental impact. Defining the ecotoxicity parameters of the main classes of raw materials in rinse-off cosmetic products can enable the development of products that are more environmentally sustainable, prioritizing substances with less environmental impact.

Objectives To compare readmission rates to the intensive care unit (ICU) before and after the implementation of a rapid response team (RRT), and to identify risk factors for readmission. Design A quasi-experimental before–after study was carried out. Setting A University Hospital. Patients All patients discharged from the ICU from January to December 2008 (control group) and from January 2010 to December 2012 (intervention group). Intervention Implementation of an RRT. Main variables of interest The data included demographic parameters, diagnoses upon admission, ICU readmission, APACHE II, SOFA, and TISS 28 scores, and routine daily assessment by an RRT of patients discharged from the ICU. Results During the study interval, 380 patients were analyzed in the period prior to the implementation of the RRT and 1361 after implementation. There was a tendency toward decreased readmission rates one year after RRT implementation. The APACHE II score and SOFA score at ICU discharge were independent factors associated to readmission, as well as clinical referral to the ICU. Conclusions The RRT intervention resulted in a sustained decrease in readmission rates one year after implementation of this service. The use of a specialized team in health institutions can be recommended for ICU survivors.

RESUMO O fato de possuir espécies com potencial econômico ilimitado, faz com que o ambiente amazônico se torne alvo constante da biopirataria e da extração predatória dos recursos. Muitas espécies deste ambiente sequer possuem catalogação e, mesmo assim, encontram risco eminente de desaparecimento. Neste âmbito, trabalhos que reúnam dados sobre estas espécies possuem grande valor científico, cultural e econômico, sendo este o estímulo que desencadeou a produção desta revisão. Aqui a espécie Calycophyllum spruceanum (Benth.) Hook. f. ex K. Schum., uma Rubiaceae amazônica, é contemplada por meio de características botânicas, indicações etnofarmacológicas e propriedades químicas. Popularmente conhecida como mulateiro, a espécie é frequentemente receitada na etnomedicina como cicatrizante e rejuvenescedor, além de ser usada no controle de manchas de pele. Como peculiaridade botânica, mulateiro apresenta tronco retilíneo com epiderme fina esverdeada que evolui para uma periderme castanho-escuro, que é anualmente renovada. Em termos químicos, há destaque para a presença de alcalóides, taninos e, sobretudo, secoiridóides (7-metoxididerrosideo, 6´-acetil-β-D-glucopiranosildiderrosideo e 8-0-tigloildiderrosideo são peculiares à espécie). Mesmo com propriedades fotoprotetoras comprovadas, C. spruceanum ainda carece de pesquisas, sobretudo àquelas voltadas para a produção ex situ da planta e àquelas que demonstrem a relação entre a ecologia da planta e a produção de metabólitos funcionais para a indústria. ABSTRACT The Amazonian environment is a constant target for biopiracy and predatory extraction of resources due to the species with vast economic potential. Many of these species have not even been cataloged, and yet are already at imminent risk of extinction. In this paper, Calycophyllum spruceanum (Benth.) Hook. f. ex k. Schum., an Amazonian Rubiaceae, is studied with information on its botanical characteristics, ethnopharmacological uses, and chemical properties. Popularly known as mulateiro, the species is often prescribed in ethnomedicine for healing and vitality, and is also used to control skin patches. As a botanical peculiarity, it has a thin green epidermis that evolves into a dark brown periderm, which is renewed annually. In terms of chemistry, there is emphasis on the presence of alkaloids, tannins, and, especially, secoiridoids (7-methoxydiderroside, 6′-acetyl-β-D-glucopyranosyldiderroside, and 8-O-tigloyldiderroside are peculiar to the species). Even with proven photoprotective properties, research on C. spruceanum is still lacking, in particular studies aimed at the ex situ production of the plant and those that show the relationship between the plant’s ecology and the production of functional metabolites for the industry.

BackgroundThe prevalence of crack cocaine on Brazil reveal an increase since 2009 and represents a public health problem.AimsTo describe and study the socio-demographic profile of female crack users undergoing treatment in psychosocial care center for alcohol and others drugs (CAPS AD).MethodsThis is a qualitative, ethnographic study of 9 female crack users that was conduct from February until September 2015 at CAPS AD in Brazil. This is study is part of a research entitled “The daily life of female crack users: public service access”.ResultsThese women are young, have children that most of them are separated and have at least primary school. All of them have a long time using crack and different attempts of abstinence. They access different public health programs.ConclusionThe results show the vulnerable profile of female crack users. These findings indicate the need specific public intervention in other to improve social and health conditions.Disclosure of interestThe authors have not supplied their declaration of competing interest.

In the present study, three different methods were used to identify yeast isolated from Trás-os-Montes, Portuguese honey. A total of 24 isolates were identified using a partial sequence of the 26S rRNA gene (rDNA), restriction patterns generated from the region spanning the internal transcribed spacers (ITS1 and ITS2) of the 5.8S rRNA gene and the API 20C AUX kit. Nine different yeast species were identified representing six different genera. Among the isolated honey samples, Rhodotorula mucilaginosa, Candida magnoliae and Zygosaccharomyces mellis were the predominant species. Partial sequence of the 26S rDNA yielded the best results in terms of correct identification, followed by-5.8S-ITS analysis. The commercial identification kit API 20C AUX was able to correctly identify only 58% of the isolates. Two new 5.8S-ITS profiles were described, corresponding to Trichosporon mucoides and Candida sorbosivorans. En este estudio, se han utilizado tres métodos para identificar levaduras aisladas de la miel de Trás-os-Montes, Portugal. Se han identificado un total de 24 aislados usando una secuencia parcial del gen rRNA 26S (rDNA), los patrones de restricción generados de la región de los espaciadores transcritos internos (ITS1 e ITS2) del gen rRNA 5.8S (rDNA) y el kit API 20C AUX. Fueron identificadas nueve especies distintas de levaduras que representan seis géneros distintos. Entre las muestras aisladas de la miel, Rhodotorula mucilaginosa, Candida magnoliae y Zygosaccha-romyces mellis eran las especies predominantes. La secuencia parcial del rDNA 26S rindió los mejores resultados para la correcta identificación, seguida por el análisis del 5.8S-ITS. El kit comercial de identificación API 20C AUX. sólo identificó correctamente el 58% de los aislados. Se describen dos perfiles nuevos de 5.8S-ITS, correspondiendo a Trichosporon mucoides y Candida sorbosivorans.

Cowpea mosaic virus (CPMV) moves from cell to cell as virus particles which are translocated through a plasmodesmata-penetrating transport tubule made up of viral movement protein (MP) copies. To gain further insight into the roles of the viral MP and capsid proteins (CP) in virus movement, full-length and truncated forms of the IMP were expressed in insect cells using the baculovirus expression system. Using ELISA and blot overlay assays, affinity purified IMP was shown to bind specifically to intact CPMV virions and to the large CP, but not to the small CP. This binding was not observed with a C-terminal deletion mutant of the IMP, although this mutant retained the capacity to bind to other MP molecules and to form tubules. These results suggest that the C-terminal 48 amino acids constitute the virion-binding domain of the MP.

A determinação da lâmina correta de irrigação para a produção de frutos de tomate é de fundamental importância para a obtenção de maiores produtividades e frutos com maior qualidade. O objetivo deste trabalho foi avaliar o desenvolvimento e a produtividade do tomate de mesa híbrido Natália sob lâminas de irrigação por gotejamento e duas formas de adubação. O experimento foi conduzido na área experimental da Universidade Federal de Goiás, campus de Jataí. O delineamento utilizado foi o de blocos casualisados em arranjo fatorial 4 × 2, referentes a quatro lâminas de irrigação (66, 100, 133, 166%) da evapotranspiração de referência diária do local (ETo), calculadas diariamente pelo método de Penman – Monteith e duas formas de adubação (via fertirrigação e adubação convencional). As variáveis analisadas foram altura de plantas e diâmetro de caule aos 30, 45, 60 e 80 dias após o transplantio, produtividade total, produtividade comercial e não comercial. Pelos resultados verifica-se que o diâmetro caulinar das plantas não foi influenciado pelas lâminas de irrigação e formas de adubação. A fertirrigação proporcionou aumento de produtividade comercial de frutos em relação à adubação convencional, quando foi aplicada a lâmina correspondente a 66% da ETo e proporcionou aumento na altura das plantas com a lâmina de 100% da ETo. Conclui-se que a lâmina correspondente a 100% da Eto proporcionou maior crescimento de plantas, aumento na produtividade total e comercial do tomateiro.

Depression is a major mental illness affecting the elderly. About 15% of the elderly have some depressive symptoms and about 2% have severe depression. This study was conducted in an Extension Program of the Federal University of the State of Rio de Janeiro (UNIRIO) and the aims are: to identify the prevalence of depression in the elderly attended by the community center “Project Rebirth” in Rio de Janeiro-RJ, and discuss strategies developed in consultation to care the depression.MethodologyThe elderly are registered in the program and accompanied by a multidisciplinary team. In nursing consultations, whose base is in the realization of Nursing Process, there is the application of the Geriatric Depression Scale named Yesavage – reduced version (GDS-15) held by students under the supervision of the teacher. After classification of the patient on the scale, they develop the care plan based in the Nursing Interventions Classification (NIC), and the nursing diagnoses that have among their factors related to depression and its symptoms.Results and conclusionsIn addition to interventions carried out in consultation, involving recovery of self-esteem, encouragement of leisure and recreational activities, and supported self-care, the program staff (psychologists, doctors, nurses and occupational therapists) works in partnership in support group health education, encouraging the integration of the elderly. The well-being and self-esteem of the elderly, thus, reduce the triggers of depression, with the integration of participants, students and professionals.Disclosure of interestThe authors have not supplied their declaration of competing interest.

Anxiety in elderly can be diagnosed based on a detailed history and cognitive examination using various instruments. These instruments aim to obtain information that supports both the syndromic and etiological diagnosis and the planning and execution of therapeutic and rehabilitation measures to be used in each case. During the nursing consultation with elderly people in the Federal University of State of Rio de Janeiro (UNIRIO), many patients with cognitive impairment showed anxious behavior. The study objectives to describe the factors related to the nursing diagnose anxiety in elderly patients attended in the nursing consultation. The consultations are based in the application of the nursing process. After the symptoms analysis, the nursing diagnosis anxiety is complemented with the related factors. The principals are death threat (64%), stressors (19%) and not needs met (17%). The data found are associated with cognitive impairment. Cognitive impairment in the elderly can lead to anxiety, depression and hopelessness, according to many studies.ConclusionsWorking the cognitive needs, and reducing stressors are appropriate strategies to reduce the anxiety. The care with the health professionals is important to notice the related factors early and improve the strategies.Disclosure of interestThe authors have not supplied their declaration of competing interest.

Five new genera, Bipuncticoris, Kiwimiris, Monopharsus, Tuicoris, and Wekamiris, and 22 new species of endemic New Zealand Mirinae, Mirini are described and figured. The 14 new species of Bipuncticoris are: cassinianus, chlorus, convexus, gurri, irroratuts, lineatus, longicerus, minor, olearinus, planus, robustus, triplex, vescus, and xestus. The five new species of Kiwimiris are: bipunctatus, coloratus, concavus, melanocerus, and niger. A single new species is described for three genera—Monopharsus annulatus, Tuicoris excelsus, and Wekamiris auropilosus. Keys to species of Bipuncticoris and Kiwimiris are provided. Biological information on host plants is given. A distinctive character in the vesica of species of Bipuncticoris is a sclerite bearing a single ring of large spines.

A new genus and five new species of Hemiptera, Miridae, from south of Brazil are described as follows: Derophthalma vittinotata, sp.n.; Parachius pallidus, sp.n.; Paranacoris, gen.n.; Paranacoris vertexalbus, sp.n.; Tropidosteptes vittiscutellatus, sp.n.; Tupiniquinus alboantennatus, sp.n. Illustrations of habitus and male genitalia are included.

Six new species of Hemiptera, Miridae, from South America are described as follows: Chrysodasia pronotata, sp.n.; Ellenia pallida, sp.n.; Horcias niger, sp.n.; Horciasoides ecuatorianus, sp.n.; Neostenotus villensis, sp.n.; Tropidosteptes scutellatus, sp.n. Figures of habitus and male genitalia are included.

The movement protein (MP) of Cowpea mosaic virus forms tubules in plasmodesmata to enable the transport of mature virions. Here it is shown that the MP is capable of specifically binding riboguanosine triphosphate and that mutational analysis suggests that GTP binding plays a role in the targeted transport of the MP. Furthermore, the MP is capable of binding both single-stranded RNA and single-stranded DNA in a non-sequence-specific manner, and the GTP- and RNA-binding sites do not overlap.

Alcohol has been shown to reduce serum calcium concentrations in several animal studies. In humans, using relatively low doses of alcohol, however, the results were inconclusive. In addition, the effect of alcohol consumption on serum magnesium concentrations is controversial. To elucidate the influence of alcohol ingestion on serum calcium and magnesium in humans 43 intoxicated patients and seven healthy volunteers who had not previously ingested alcohol were studied. There was an inversely related diminuition of serum calcium and magnesium concentrations with increasing serum alcohol. These effects of alcohol may play a role in the metabolic and clinical disorders observed in severely intoxicated people.

Summary A new species of Dolichomiris Reuter from India and another from South Africa are described, while costicollis Berg is excluded, bringing the total number of species in the genus to seven. All species are figured and keyed. D. linearis Reuter is recorded from Australia, New Guinea, Indonesia and the New Hebrides. Lectotypes are designated where necessary. A revised key to the genera of Stenodemini is presented.

Three genera and 14 species of Deraeocorinae are now known from New Zealand. Nine new species of Romna Kirkaldy, R. albata, R. bicolor, R. cuneata, R. nigrovenosa, R. oculata, R. or nata, R.pallida, R. uniforms, and R. variegata are described and figured (as are the five previously known species). Keys to the genera of Deraeocorinae in New Zealand and to the 12 species of Romna are provided. Lectotypes and type localities are designated for Morna capsoides White and Morna scotti White, and a type locality for Romna marginicollis (Reuter).