165 results on '"Budakov, Dragana"'
Search Results
2. The Pesticide Market in Serbia
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Milić Dragan, Novaković Tihomir, Grahovac Mila, Budakov Dragana, Grahovac Jovana, Vlajkov Vanja, Loc Marta, and Tekić Dragana
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biopesticides ,pesticide market ,serbia ,Agriculture - Abstract
The use of plant protection products (PPPs) has become a social concern regarding food safety and quality, and risks to human health and the environment. Accordingly, the agricultural microbials market is a steadily growing sector of the global agriculture industry. This paper evaluates the market of basic groups of pesticides in Serbia (namely herbicides, fungicides and insecticides), as well as other collectively presented plant protection products, with a particular emphasis on the biopesticides segment. The official data of the Serbian Plant Protection Directorate for the period 2000-2020 served as a primary source of data. The movement of PPPs and active ingredients (AIs) imports into Serbia were analyzed separately. The average values of PPP quantities imported into Serbia were computed for the period under consideration. The time series variability was evaluated using the coefficient of variation, whereas the dynamics of PPP import movements was evaluated using the rate of change. In the overall structure of the imported pesticides in Serbia, herbicides remain the dominant product type (57.0%), followed by fungicides (27.5%), insecticides (10.5%), and other PPPs (5.0%). The import of AIs for the production of herbicides claimed a share of 79.8%, whereas the share of AIs for the fungicide production approximated to 12.8% (followed by AIs for the insecticide production (6.7%) and AIs for the other groups of pesticides (0.7%). Biopesticides comprise a small share of the total crop protection market in Serbia. Bioinsecticides were found to be imported into Serbia in largest quantities (followed by biofungicides, bioacaricides, and biobactericides). No imports of bioherbicides have been recorded. According to the official data on the import of PPPs in Serbia in the period 2000–2020, it can be concluded that there is a positive growth trend, which is expected to continue in the future. Although the import of AIs shows a positive rate of change, no significant growth trends were recorded.
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- 2022
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3. Impact of Simultaneous Nutrient Priming and Biopriming on Soybean Seed Quality and Health.
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Tamindžić, Gordana, Miljaković, Dragana, Ignjatov, Maja, Miladinović, Jegor, Đorđević, Vuk, Milošević, Dragana, Jovičić, Dušica, Vlajić, Slobodan, Budakov, Dragana, and Grahovac, Mila
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SUSTAINABLE agriculture ,FIELD crops ,BACILLUS megaterium ,SEED quality ,SEED viability ,GERMINATION - Abstract
In soybean production, numerous strategies are utilized to enhance seed quality and mitigate the effects of biotic and abiotic stressors. Zn-based nutrient priming has been shown to be effective for field crops, and biopriming is a strategy that is becoming increasingly important for sustainable agriculture. On the other hand, there is a lack of information about the effect of comprehensive nutrient priming and biopriming techniques on soybean seed quality and viability and seed health. This study was performed to assess the benefits of nutrient priming with Zn, biopriming with Bacillus megaterium and Bradyrhizobium japonicum (single and co-inoculation), and combination of nutrient priming and biopriming on the seed quality and viability, as well as seed infection caused by Alternaria spp. and Fusarium spp. Three different laboratory tests were employed: germination test, accelerated aging test, and seed health test. The results revealed that all tested priming treatments have a beneficial effect on seed germination, initial plant growth, and reduction of seed infection in normal and aged seeds. Additionally, comprehensive priming with Zn, Bacillus megaterium, and Bradyrhizobium japonicum reduced the occurrence of Alternaria spp. (−84% and −75%) and Fusarium spp. (−91% and −88%) on soybean seeds in the germination and accelerated aging tests, respectively, as compared to the control, which proved to be the most effective treatment in both optimal and stressful conditions. [ABSTRACT FROM AUTHOR]
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- 2024
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4. First Report of Brown Spot on Stored Apple Fruits Caused by Stemphylium vesicarium in Serbia
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Petreš, Mladen, primary, Loc, Marta, additional, Budakov, Dragana, additional, Dudaš, Tatjana, additional, Stojšin, Vera, additional, Petreš, Aleksandra Stankov, additional, and Grahovac, Mila, additional
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- 2024
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5. First Report of Brown Spot on Stored Apple Fruits Caused by Stemphylium vesicarium in Serbia
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Petreš, Mladen, Petreš, Mladen, Loc, Marta, Budakov, Dragana, Dudaš, Tatjana, Stojšin, Vera, Stankov Petreš, Aleksandra, Grahovac, Mila, Petreš, Mladen, Petreš, Mladen, Loc, Marta, Budakov, Dragana, Dudaš, Tatjana, Stojšin, Vera, Stankov Petreš, Aleksandra, and Grahovac, Mila
- Abstract
Apple is one of the most economically important fruit crops worldwide, and postharvest fungal diseases can cause significant losses during storage (Petreˇs et al. 2020). Apple fruits (cv. Fuji) with necrosis symptoms were collected during the fall of 2022 from an ultra-low-oxygen cold storage facility in Titel, Serbia. The fruits originated from the apple orchards in Titel (45°12947.10N, 20°15923.60E). The pathogens were isolated from collected fruit samples using standard phytopathological techniques. Fruits were surface-sterilized, rinsed with sterile water, and aseptically cut in half, and small fragments collected from the border of healthy and diseased tissue were placed on potato dextrose agar (PDA) medium in Petri dishes and incubated at 25 ± 1°C in the dark for 7 days. The obtained 11 isolates were identified to the genus level as Alternaria (incidence, 46%), Penicillium (36%), Fusarium (9%), and Stemphylium (9%) based on their morphological characteristics. Pathogenicity of all the isolates was confirmed on apple fruits of cultivars Fuji and Golden Delicious. The fruits were surface-sterilized, sprayed with 5 ml of conidial suspension (1 × 105 conidia/ml), and incubated at room temperature for 21 days. Symptoms developed on inoculated fruits were the same as symptoms observed on apple fruit samples collected from cold storage.
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- 2024
6. Assessment of Various Nanoprimings for Boosting Pea Germination and Early Growth in Both Optimal and Drought-Stressed Environments
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Tamindžić, Gordana, Tamindžić, Gordana, Azizbekian, Sergei, Miljaković, Dragana, Ignjatov, Maja, Nikolić, Zorica, Budakov, Dragana, Vasiljević, Sanja, Grahovac, Mila, Tamindžić, Gordana, Tamindžić, Gordana, Azizbekian, Sergei, Miljaković, Dragana, Ignjatov, Maja, Nikolić, Zorica, Budakov, Dragana, Vasiljević, Sanja, and Grahovac, Mila
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One of the main climate change-related variables limiting agricultural productivity that ultimately leads to food insecurity appears to be drought. With the use of a recently discovered nanopriming technology, seeds can endure various abiotic challenges. To improve seed quality and initial growth of 8-day-old field pea seedlings (cv. NS Junior) under optimal and artificial drought (PEG-induced) laboratory conditions, this study aimed to assess the efficacy of priming with three different nanomaterials: Nanoplant Ultra (Co, Mn, Cu, Fe, Zn, Mo, and Se), Nanoplant Ca-Si (Ca, Si, B, and Fe), and Nanoplant Sulfur (S). The findings indicate that nanopriming seed treatments have a positive impact on seed quality indicators, early plant growth, and drought resilience in field pea plants established in both optimal and drought-stressed conditions. Nevertheless, all treatments showed a positive effect, but their modes of action varied. Nanoplant Ultra proved to be the most effective under optimal conditions, whereas Nanoplant Ca-Si and Nanoplant Sulfur were the most efficient under drought stress. After a field evaluation, the examined comprehensive nanomaterials may be utilized as priming agents for pea seed priming to boost seed germination, initial plant growth, and crop productivity under various environmental conditions.
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- 2024
7. Sensitivity of Cercospora beticola Isolates to Azoxystrobin
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Balandžić Milijanka, Stojšin Vera, Grahovac Mila, Bagi Ferenc, Petreš Mladen, Loc Marta, Dudaš Tatjana, Vukotić Jelena, Savić Zagorka, Stankov Aleksandra, and Budakov Dragana
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sugar beet ,leaf spot ,cercospora beticola ,azoxystrobin ,fungicide ,resistance ,qoi ,Agriculture - Abstract
Sugar beet leaf spot, caused by the air-borne fungus Cercospora beticola Sacc., leads to a decrease in sugar beet leaf mass and the consequent regrowth of leaves based on exploiting the sugar reserves stored in the plant’s roots, thus ultimately resulting in lower yields and sugar contents of sugar beets. Azoxystrobin belongs to the group of QoI fungicides, which inhibit mitochondrial respiration by blocking cytochrome c reductase. The QoI fungicides are characterized by a very high risk of resistance interfering with their biological activity. For the purpose of testing the azoxystrobin sensitivity of the Cercospora beticola population found at the site of Rimski Šančevi, a collection of 84 isolates was assembled and tested for sensitivity to azoxystrobin by measuring the mycelial growth on fungicide-amended media with the addition of SHAM. The results obtained indicate that none of the isolates tested exhibited complete sensitivity to azoxystrobin, 4% were found to have reduced sensitivity, 26% were moderately resistant and 70% were highly resistant. A higher proportion of resistant isolates recorded is associated with the loss of azoxystrobin biological efficacy at the experimental site.
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- 2020
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8. Visual, instrumental, mycological and mycotoxicological characterization of wheat inoculated with and protected against Alternaria spp.
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Janić-Hajnal Elizabet P., Belović Miona M., Plavšić Dragana V., Mastilović Jasna S., Bagi Ferenc F., Budakov Dragana B., and Kos Jovana J.
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colour ,wheat ears ,wheat kernels ,field fungi ,Alternaria toxins ,Chemical technology ,TP1-1185 - Abstract
The aim of this work was to characterize visual properties, instrumentally measured colour properties, field fungi presence and Alternaria toxins levels in wheat samples grown under conditions aimed at inhibition and stimulation of wheat infection with fungi from the Alternaria genus. Experiment was carried out on the wheat treated by fungicide and wheat inoculated by Alternaria spp., while non treated wheat was used as a control. Statistically significant difference was observed between all three treatments using visual scale. Protected wheat samples were significantly different from other samples in terms of all measured colour parameters while inoculated and control wheat samples were significantly different in terms of lightness and dominant wavelength. Identification of field fungi in the all examined wheat samples showed that the dominant mycotoxigenic fungus was Alternaria spp., followed by Fusarium spp. The content of Alternaria toxins in samples of wheat hulls and dehulled kernels point out at higher concentrations of Alternaria toxins in hulls than in dehulled kernels. [Projekat Ministarstvo nauke Republike Srbije, br. III 46001 i br. III 46005]
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- 2016
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9. The Effect of Cultivation Conditions on Antifungal and Maize Seed Germination Activity of Bacillus-Based Biocontrol Agent
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Vlajkov, Vanja, primary, Pajčin, Ivana, additional, Loc, Marta, additional, Budakov, Dragana, additional, Dodić, Jelena, additional, Grahovac, Mila, additional, and Grahovac, Jovana, additional
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- 2022
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10. Genetic Diversity of Pectobacterium spp. on Potato in Serbia
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Loc, Marta, primary, Milošević, Dragana, additional, Ivanović, Žarko, additional, Ignjatov, Maja, additional, Budakov, Dragana, additional, Grahovac, Jovana, additional, and Grahovac, Mila, additional
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- 2022
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11. Ectomycorrhizal Fungi Modulate Biochemical Response against Powdery Mildew Disease in Quercus robur L.
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Kebert, Marko, primary, Kostić, Saša, additional, Zlatković, Milica, additional, Stojnic, Srdjan, additional, Čapelja, Eleonora, additional, Zorić, Martina, additional, Kiprovski, Biljana, additional, Budakov, Dragana, additional, and Orlović, Saša, additional
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- 2022
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12. EKONOMSKI ZNAČAJNI VIRUSI PAPRIKE UTVRĐENI NA PODRUČJU REPUBLIKE SRPSKE.
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Babić, Gordana, Trkulja, Vojislav, Budakov, Dragana, Iličić, Renata, Mališević, Rade, and Bagi, Ferenc
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- 2023
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13. Medium for the Production of Bacillus-Based Biocontrol Agent Effective against Aflatoxigenic Aspergillus flavus: Dual Approach for Modelling and Optimization
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Vlajkov, Vanja, primary, Anđelić, Stefan, additional, Pajčin, Ivana, additional, Grahovac, Mila, additional, Budakov, Dragana, additional, Jokić, Aleksandar, additional, and Grahovac, Jovana, additional
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- 2022
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14. Sugar beet virus diseases in Serbia
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Budakov, Dragana, Budakov, Dragana, Stojšin, Vera, Ćurčić, Živko, Dudaš, Tatjana, Nagl, Nevena, Bagi, Ferenc, Grahovac, Mila, Budakov, Dragana, Budakov, Dragana, Stojšin, Vera, Ćurčić, Živko, Dudaš, Tatjana, Nagl, Nevena, Bagi, Ferenc, and Grahovac, Mila
- Abstract
During 2017, yellowing of sugar beet leaves occurred in sugar beet fields in Serbia. Disease was visible on individual plants which had symptoms of intercostal yellowing, leaves were upright and brittle and in time this yellowing turned into necrosis. For the purpose of this research leaves with these symptoms were collected from 40 fields and analyzed using ELISA on the presence of Turnip Yellows Virus (Beet mild yellowing virus), Beet Mosaic Virus, Beet Necrotic Yellow Vein Virus and Beet yellows virus. Results proved occurrence of these viruses and were commented in regard to meteorological data which were favorable for vector activity.
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- 2020
15. Influence of sugar beet cultivar and NKP nutrition on Cercospora leaf spot
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Budakov, Dragana, Budakov, Dragana, Stojšin, Vera, Ćurčić, Živko, Dudaš, Tatjana, Stankov, Aleksandra, Bagi, Ferenc, Grahovac, Mila, Budakov, Dragana, Budakov, Dragana, Stojšin, Vera, Ćurčić, Živko, Dudaš, Tatjana, Stankov, Aleksandra, Bagi, Ferenc, and Grahovac, Mila
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Cercospora beticola is economically the most important sugar beet leaf disease primarily controlled by fungicide applications. However, other factors influence its incidence as well, some of which are the choice of cultivar and plant nutrition. These factors have been put together in field trial, which tested reaction of eight sugar beet genotypes (G1-G8) and 20 variants of NPK nutrition.
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- 2020
16. Uticaj etarskih ulja i hidrolata etarskih ulja na razvoj prouzrokovača gorke truleži jabuke
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Petreš, Mladen, Petreš, Mladen, Aćimović, Milica, Loc, Marta, Dudaš, Tatjana, Budakov, Dragana, Stojšin, Vera, Grahovac, Mila, Petreš, Mladen, Petreš, Mladen, Aćimović, Milica, Loc, Marta, Dudaš, Tatjana, Budakov, Dragana, Stojšin, Vera, and Grahovac, Mila
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Vrste roda Colletotrichum, prouzrokovači gorke truleži jabuke, predstavljaju jedne od najznačajnijih prouzrokovača truleži plodova jabuke u skladištu. Zbog dominantne zastupljenosti u odnosu na druge vrste ovog roda posebno su značajne vrste C. acutatum i C. gloeosporioides. S obzirom da upotreba fungicida nakon berbe i tokom skladištenja nije dozvoljena zbog njihovih nepovoljnih ekotoksikoloških svojstava, a plodovi ostaju nezaštićeni tokom skladištenja, neophodno je definisanje alternativnih rešenja. Prethodna istraživanja su dokazala značajan antifungalni potencijal etarskih ulja i njihovih hidrolata (sporednih proizvoda koji se dobijaju pri proizvodnji etarskih ulja), te mogu predstavljati adekvatnu zamenu fungicidima. U ogledu je ispitan uticaj etarskih ulja čubra (Satureja hortensis), bosiljka (Ocimum basilicum), muskatne žalfije (Salvia sclarea) i divljeg origana (Origanum vulgare), kao i hidrolata etarskih ulja čubra, bosiljka i muskatne žalfije na izolate KA7 (C. acutatum) i MRMCD6 (C. gloeosporioides).
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- 2022
17. Etarska ulja u suzbijanju prouzrokovača vlažne truleži krtola krompira iz roda Pectobacterium
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Loc, Marta, Loc, Marta, Grahovac, Mila, Aćimović, Milica, Petreš, Mladen, Budakov, Dragana, Dudaš, Tatjana, Stojšin, Vera, Milošević, Dragana, Loc, Marta, Loc, Marta, Grahovac, Mila, Aćimović, Milica, Petreš, Mladen, Budakov, Dragana, Dudaš, Tatjana, Stojšin, Vera, and Milošević, Dragana
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Vrste roda Pectobacterium su proteklih godina postale jedne od deset najproučavanijih fitopatogenih bakterija. Pažnju zavređuju zbog sve učestalije pojave novih vrsta, ekstremnoj adaptivnosti različitim domaćinima i uslovima životne sredine. Intenzivna trgovinska razmena dovodi do povećanja mogućnosti dospevanja novih vrsta na udaljena područja proizvodnje krompira, uzimajući u obzir da je glavni izvor inokuluma zaražen vegetativni materijal namenjen za sadnju. Štete uzrokovane vrstama Pectobacterium dosežu velike razmere, s obzirom na to da svoju aktivnost nastavljaju i po završetku vegetacije – prilikom transporta, prerade i u uslovima skladišta. Suzbijanje ovih patogena postaje ekstremno zahtevno uzimajući u obzir opšti nedostatak baktericida, te su mnogobrojni napori učinjeni sa ciljem iznalaženja načina suzbijanja prouzrokovača vlažne truleži krtola u uslovima skladišta. Mnoga istraživanja ukazuju na visoku efikasnost biljnih ekstrakata i etarskih ulja, kao moćnih agenasa zaštite. U cilju utvrđivanja efekata etarskih ulja (EU) bosiljka (Ocimum basilicum), čubra (Satureja hortensis), divljeg origana (Origanum vulgare), vreska (Calluna vulgaris) i timjana (Thymus vulgaris) na vrste P. carotovorum (Pc), P. brasiliense (Pb) i P. punjabense (Pp), sproveden je ogled in planta, na krtolama krompira sorte Lady Claire, jedne od najosetljivijih sorti na Pectobacterium spp.
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- 2022
18. Osetljivost Macrophomina phaseolina prema Bacillus sp. i Trichoderma harzianum in vitro
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Stankov Petreš, Aleksandra, Stankov Petreš, Aleksandra, Budakov, Dragana, Stojšin, Vera, Nagl, Nevena, Grahovac, Mila, Ćurčić, Živko, Stankov Petreš, Aleksandra, Stankov Petreš, Aleksandra, Budakov, Dragana, Stojšin, Vera, Nagl, Nevena, Grahovac, Mila, and Ćurčić, Živko
- Abstract
Primena fungicida u suzbijanju zemljišnih patogena ne predstavlja efikasnu i ekonomski opravdanu meru. Takođe, oni mogu da naruše balans korisnih mikroorganizama u zemljištu, dok njihove rezidue utiču na zagađenje životne sredine. U nastojanju da se smanji upotreba sintetičkih fungicida, primena bioloških agenasa može imati važnu ulogu u „eco-frendly“ poljoprivrednim sistemima. Biološki agensi kao što su vrste iz rodova Bacillus i Trichoderma se najčešće upotrebljavaju u biološkoj borbi protiv zemljišnih patogena. Cilj ovog istraživanja je bio da se ispita antagonistički uticaj Bacillus sp. i Trichoderma spp. na izolate Macrophomina phaseolina (ŠR55(3)/09 i ŠR23/11) u in vitro uslovima. Za ispitivanje je korišten Bacillus sp., 74 koji je komercijalno dostupan kao mikrobiološko đubrivo pod nazivom Bacillomix® Original (Bacillomix Co., Srbija). Metodom inkorporacije komercijalne suspenzije mikrobiološkog đubriva u KDA hranljivu podlogu ispitane su koncentracije preparata od 1, 2, 5 i 10%. U cilju ispitivanja osetljivosti M. phaseolina prema Trichoderma spp. korišteno je 13 izolata koji su predhodno identifikovani kao Trichoderma harzianum. Test dvojne kulture (TDK) je korišten kako bi utvrdili antagonistički uticaj ispitivanih izolata.
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- 2022
19. Ectomycorrhizal fungi modulate biochemical response against powdery mildew disease in Quercus robur L.
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Kebert, Marko, Kebert, Marko, Kostić, Saša, Zlatković, Milica, Stojnić, Srđan, Čapelja, Eleonora, Zorić, Martina, Kiprovski, Biljana, Budakov, Dragana, Orlović, Saša, Kebert, Marko, Kebert, Marko, Kostić, Saša, Zlatković, Milica, Stojnić, Srđan, Čapelja, Eleonora, Zorić, Martina, Kiprovski, Biljana, Budakov, Dragana, and Orlović, Saša
- Abstract
In light of climate change, pedunculate oak (Q. robur L.) was marked as the most threatened European tree species. Pedunculate oak is particularly jeopardized by powdery mildew disease caused by Erysiphe alphitoides. We hypothesized that priming of this tree species with ectomycorrhizal fungi could mitigate biotic stress and produce bioprotective properties against the disease. In this study, we have compared oaks’ foliar physiological and biochemical responses upon infection with E. alphitoides in the presence and absence of ectomycorrhizal fungi (ECM). The main aim of this study was to inspect how ECM modulate an oak’s biochemical response to infection with E. alphitoides, particularly at the level of the accumulation of the main polyamines (putrescine, spermidine, and spermine), soluble osmolytes (proline and glycine betaine), and phenolics (total phenolic content, flavonoids, and condensed tannins). A polyamine quantification was performed after derivatization by using high-performance liquid chromatography (HLPC) coupled with fluorescent detection. Oak seedlings inoculated with ECM fungi exhibited significantly higher levels of putrescine, spermine, and proline compared to non-inoculated seedlings, indicating the priming properties of the ECM. E. alphitoides caused an increase in individual and total polyamine content and lipid peroxidation in oak leaves regardless of the effect of ECM, while causing a decrease in physiological and antioxidative parameters and water use efficiency (WUE). Common biochemical parameters may contribute to understanding the underpinning plant defense mechanisms in three-way interactions among plants and pathogenic and ectomycorrhizal fungi and can be used as reliable adaptability descriptors in the context of climate change.
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- 2022
20. Essential oils and hydrolates in control of plant pathogens
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Grahovac, Mila, Grahovac, Mila, Aćimović, Milica, Budakov, Dragana, Stojšin, Vera, Loc, Marta, Petreš, Mladen, Dudaš, Tatjana, Grahovac, Mila, Grahovac, Mila, Aćimović, Milica, Budakov, Dragana, Stojšin, Vera, Loc, Marta, Petreš, Mladen, and Dudaš, Tatjana
- Abstract
Under conditions of intensive plant production and intensive international trade with plant material, coupled with climate changes, plant pathogens became one of the major limiting factors in food and feed production. Besides these extremely favorable conditions for reproduction and dissemination of plant pathogens, resistance development to available control tools and ecotoxicological properties of these tools show that plant pathogen control has never been more challenging. Development of alternative, eco-friendly control tools against phytopathogens, became a necessity of contemporary agriculture. Essential oils are mixtures of complex volatile compounds. They are synthesized through secondary metabolic pathways in many plant species. Essential oils are normally extracted by hydro-distillation or steam distillation of plant tissues. Hydrolates are co-products of the distillation formed in significantly higher quantities than the target substance. Hydrolate represents aromatic water containing approximately 0.1% essential oil compounds.
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- 2022
21. First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia
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Loc, Marta, Loc, Marta, Milošević, Dragana, Ignjatov, Maja, Ivanović, Žarko, Budakov, Dragana, Grahovac, Jovana, Vlajkov, Vanja, Pajčin, Ivana, Grahovac, Mila, Loc, Marta, Loc, Marta, Milošević, Dragana, Ignjatov, Maja, Ivanović, Žarko, Budakov, Dragana, Grahovac, Jovana, Vlajkov, Vanja, Pajčin, Ivana, and Grahovac, Mila
- Abstract
Soft rot and blackleg are common diseases of potato (Solanum tuberosum) in Serbia. Opportunistic pectinolytic plant pathogens in the genus Pectobacterium cause soft rot and wilt diseases by cell wall degradation. They cause major economic losses in potato crops worldwide and are among the top 10 plant pathogenic bacteria (Mansfield et al. 2012). Potato plants (‘VR808’) with symptoms of wilting, slow growth, stem blackening, and tuber softening were collected from a commercial field in Zobnatica, Serbia, in July 2019 and analyzed. All symptoms occurred in the same field, and ~5% of plants were symptomatic. Isolation was performed from 10 randomly chosen symptomatic plants and tubers. Plant tissue was surface disinfected, and 1-cm sections from the margins of lesions were macerated in sterile distilled water (SDW) for 25 min and streaked on nutrient agar. After 48 h of incubation at 26C, predominant shiny, cream-colored, round colonies were obtained from all samples. Three representative isolates (MMZKVR1, MMZCVR2, and MMZKVR3) from independent samples were selected randomly for biochemical and pathogenicity tests. Isolates were gram-negative, nonfluorescent facultative anaerobes exhibiting pectinolytic activity on potato tuber slices and hypersensitive response on tobacco leaves. They expressed catalase activity but did not express oxidase or acid phosphatase activity or produce indole.
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- 2022
22. Level of seed infection of cultivated sorghum with fungi from genus Fusarium
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Bodoči Karolina S., Bagi Ferenc F., Berenji Janoš J., Stojšin Vera B., Budakov Dragana B., and Nađ Livia T.
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broomcorn ,Fusarium ,grain sorghum ,disease intensity score ,Science (General) ,Q1-390 - Abstract
During the year of 2011, the level of fungi infection with the genus Fusarium was examined in seven genotypes of grain sorghum (Gold F1, A 28, B 28, Alba F1, A 73, B 73, Re 236) grown in six localities: Srbobran, Futog and four localities nearby Bački Petrovac (Bački Petrovac 1, 2, 3 and 4). The rate of seed infection with fungus Fusarium in the investigated period ranged from 0 to 8.5%. The infection did not occur in the genotype B73 (Bački Petrovac 1), while a low rate of infection of 0-3% was detected in the genotype B 28 (Srbobran). Medium rate of infection was recorded for the genotype A 28 (Srbobran) and A 28 (Futog) with 4.25% and for Gold F1 (Bački Petrovac 2) with 5.5%. High rate of infection was recorded for the genotype A 73 (Futog), A 73 (Bački Petrovac 2) (6.25%), Alba F1 (Bački Petrovac 2) (8%) and Re 236 (Futog) (8.5%). One isolate from each F. solani, F. graminearum, F. subglutinans and F. verticilliodes was selected for the pathogenicity investigation. Thirty plants were inoculated from each genotype of grain sorghum (varieties Alba F1 and Gold F1) and broomcorn (var. Reform and Prima). The spraying inoculation with the suspension of mycelium and conidia was performed when sorghum was at the end of blooming on June 19, 2012. The intensity of the infection on the sorghum panicles was reached in the phenophase of milky-wax maturity on July 8, 2012 and the scale of S h a r m a et al., (2010) was used. The symptoms of the artificial inoculation appeared only in the broomcorn. Among all isolates, the isolate of the species F. graminearum manifested the highest pathogenicity on the variety Reform with average score of 4.43 and on variety Prima with 4.17. [Projekat Ministarstva nauke Republike Srbije, br. III 46005: Genetical divergence, technological quality and storage of cereals and pseudocereals from organic production]
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- 2013
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23. Mycobiota of Serbian wheat grain in 2010
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Stanojev Zagorka N., Bagi Ferenc F., Bodroža-Solarov Marija I., Stojšin Vera B., Vučković Jovana N., Budakov Dragana B., and Bodoči Karolina S.
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wheat ,mycobiota ,contamination ,germination ,determination ,pathogenicity of isolates ,impact of sites ,Fusarium ,Alternaria ,Science (General) ,Q1-390 - Abstract
This research focused on the assessment of the infection level of sampled wheat grains with phytopathogenic fungi. The samples were taken from the localities Rimski Šančevi and Sombor. The research investigated the impact of localities to intensity of fungal infection by fungi from genus Fusarium and Alternaria. Isolates from genus Fusarium and Alternaria were determined to species level. Pathogenicity of Fusarium and Alternaria isolates from different localities to wheat seedlings was also established. [Projekat Ministarstva nauke Republike Srbije, br. III 46005: Genetic divergence, technological quality and storage of cereals and pseudocereals from organic production]
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- 2013
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24. Evaluation of rapid protocols for DNA isolation from Cercospora beticola Sacc.
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Budakov Dragana, Nagl Nevena, Taški-Ajduković Ksenija, Stojšin Vera, and Bagi Ferenc
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Cercospora ,DNA ,impurity ,isolation ,protocol ,Plant culture ,SB1-1110 ,Biotechnology ,TP248.13-248.65 - Abstract
The most fungal DNA isolation protocols are designed to obtain high amounts of very pure DNA, requiring large fungal cultures and extraction procedures with many purification steps. Since the PCR does not require high purity DNA, the aim of this investigation was to evaluate three fast and simple fungal DNA isolation protocols for further use in Cercospora PCR based research. The purity and quantity of isolated DNAs were determined spectrophotometrically, electrophoretically and by PCR reaction with universal primers. The amounts of DNA evaluated on agarose gels, isolated by protocols A and C, did not correspond to the spectrophotometrical values, probably due to RNA impurities. In samples isolated by protocol B these impurities were not detected and the DNA concentrations were more similar. Neither protocol eliminated impurities such as carbohydrates and phenol. The average DNA yield of protocol A was 1.04 μg/μl, protocol B 0.88 μg/μl, and protocol C 0.55 μg/μl. The DNA quality most suitable for PCR analysis was obtained by protocol A, where amplification product with universal primers was detected in all DNA samples. The amplification product was detected in 87% of samples isolated by protocol C and in only 60% of samples isolated by protocol B. Although DNA obtained by protocol A had the highest yield and best quality, the isolation protocol C should be also recommended, for it does not require phenol, chlorophorm or liquid nitrogen.
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- 2012
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25. Pathogenic, morphological and molecular characteristics of Alternaria Tenuissima from soybean
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Jasnić Stevan M., Marjanović Žaklina S., Vidić Miloš B., Bagi Ferenc F., Budakov Dragana B., Pavlović Snežana Đ., and Stojšin Vera B.
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Alternaria tenuissima ,soybean ,pathogenicity ,morphology ,ITS1-5.8S-ITS2 rDNA sequence ,Science (General) ,Q1-390 - Abstract
During 2008 and 2009 phytopathological isolations were done from soybean plants and seed samples from several localities in Serbia. A total of 19 isolates of Alternaria spp. were isolated, 13 from the seed and 3 from both leaf and stem. In order to determine and characterize isolates, cultural, morphological, molecular and pathogenic characteristics were thoroughly investigated. The slowest growth of the examined isolates was noted on Malt agar (MA) with average colony diameter of 42.9 mm after 7 days of incubation. On other two media (V8 and PCA), colony growth was uniform and faster, with average diameter of 66.8 mm and 66.1 mm, respectively. Isolates of fungi form unbranched or poorly branched conidial chains on short unbranched conidiophores. Conidia are dark in colour, multicellular with transverse and longitudinal septae. They are of different size regarding the place of formation in the chain. Based on these characteristics, the tested isolates were determined as Alternaria tenuissima. Molecular identification with sequencing of ITS1-5.8S-ITS2 rDNA verified that investigated isolates belong to Alternaria tenuissima group. Pathogenicity test proved that all isolates were more or less virulent to soybean seed (12.5% to 40% of rotten seeds), while pathogenicity on plants was poorly expressed.
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- 2011
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26. The effect of fungicide treatment on mycotoxin content and yield parameters of wheat
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Bagi Ferenc F., Bodroža-Solarov Marija I., Balaž Ferenc F., Mastilović Jasna S., Stojšin Vera B., Budakov Dragana B., and Lazić Sanja D.
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fungicide treatment ,mycotoxins ,Fusarium ,Science (General) ,Q1-390 - Abstract
Effects of treatment with triazole fungicide were evaluated on 14 wheat genotypes with respect to mycotoxin (aflatoxins, ochratoxin A and deoxynivalenol), yield, 1000 kernel weight and hectoliter weight. Mycopopulation of seed samples was also determined. According to the results, fungicide treatment can reduce the level of mycotoxins in seed samples in order to improve the quality parameters and reduce the level of fungal contamination.
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- 2011
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27. Susceptibility level of cucumber downy mildew (Pseudoperonospora cubensis) to metalaxyl
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Bagi Ferenc F., Balaž Ferenc F., Stojšin Vera B., Budakov Dragana B., Sokolovski Tatjana V., and Radonić Bojana K.
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cucumber downy mildew ,level of susceptibility to fungicides ,metalaxyl ,Science (General) ,Q1-390 - Abstract
Level of susceptibility of Pseudoperonospora cubensis isolate from Ratkovo to metalaxyl in concentrations 50, 100, 200, 400 and 800 μg/ml was investigated. The trials were conducted on cotyledon and fully developed young leaves using cucumber cultivar Haroš. Reduced level of susceptibility was detected in metalaxyl concentrations of 50, 100 and 200 μg/ml because the intensity of sporulation in these treatments was on the same level as in control. Sporulation was also observed on developed leaves treated with metalaxyl in concentrations of 400 and 800 μg/ml.
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- 2009
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28. Gibberella intermedia the pathogen of St. John's Wort, coneflower and marshmallow in Serbia
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Pavlović Snežana Đ., Stojšin Vera B., Stojanović Saša D., Starović Mira S., Bagi Ferenc F., and Budakov Dragana B.
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medicinal plants ,St. John's Wort ,marshmallow ,coneflower ,Gibberella intermedia ,Science (General) ,Q1-390 - Abstract
Gibberella intermedia (Kuhlmann) Samuels et al. (anamorf: Fusarium proliferatum /Matsushima/ Nirenberg) was isolated from seeds of St. John's wort, marshmallow, and coneflower, as well as from roots and stalks of marshmallow and roots of coneflower. These plants had symptoms of leaf chlorosis, malformation, withering and plant dwarfing and were collected from several localities in Serbia during five-year investigations of mycopopulations of the mentioned plants. The morphological characteristics of the pathogen were described.
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- 2009
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29. The causal agent of apple scab (Venturia inaequalis (Cooke) Winter)
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Petreš, Mladen, primary, Grahovac, Mila, additional, Budakov, Dragana, additional, Stojšin, Vera, additional, Loc, Marta, additional, Dudaš, Tatjana, additional, and Došen, Nataša, additional
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- 2022
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30. Podosphaera leucotricha (Ellis et Everhart) Salmon: Powdery mildew of apple
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Dudaš, Tatjana, primary, Vukotić, Jelena, additional, Loc, Marta, additional, Petreš, Mladen, additional, Budakov, Dragana, additional, Grahovac, Mila, additional, and Stojšin, Vera, additional
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- 2022
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31. Penicillium: Species causing blue mold on stored apple fruits
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Dudaš, Tatjana, primary, Todić, Aleksandra, additional, Budakov, Dragana, additional, Grahovac, Mila, additional, Loc, Marta, additional, and Stojšin, Vera, additional
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- 2022
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32. Correlation between lipid peroxidation and phenolics content in leaves and roots of sugar beet infected with Rhizoctonia solani
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Kiprovski, Biljana, Malenčić, Djordje, Popović, Milan, Stojšin, Vera, Budakov, Dragana, Ćurčić, Živko, and Danojević, Dario
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- 2014
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33. Identification of Rhizoctonia solani isolates from sugar beet roots by analyzing the ITS region of ribosomal DNA
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Stojšin Vera B., Budakov Dragana, Jacobsen Barry, Grimme Eva, Bagi Ferenc F., and Jasnić Stevan
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sugar beet ,Rhizoctonia solani ,anastomosis group ,ITS region ,PCR ,Science (General) ,Q1-390 - Abstract
Rhizoctonia solani (Kühn) is one of the most important sugar beet pathogens Rhizoctonia solani anastomosis groups (AGs) 2-2 and 4 are proven to be the most common pathogenic strains on sugar beet. AG 2-2 (intraspecific groups IIIB and IV) can cause root and crown rot while damping-off of seedlings is most frequently attributed to AG 4. Four isolates of R. solani from sugar beet roots showing characteristic crown and root rot symptoms, collected from different localities in Vojvodina Province, were chosen and compared to the well-characterized R. solani isolate R9, AG 2-2 IV, from the USA. All Vojvodinian isolates showed medium level of pathogenicity and were able to cause crown and root rot symptoms on inoculated sugar beet roots. Based on anastomosis reaction, isolates from Vojvodina did not belong to the AG 2-2 group. Sequencing of the ITS (internal transcribed spacer) region of ribosomal DNA was performed on the Vojvodinian isolates from R9 in order to determine their relatedness. Sequence analysis showed that these isolates were different than R9 and were closely related (99-100% sequence homology) to anastomosis group 4, subgroup HG II.
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- 2007
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34. ANTIMICROBIAL ACTIVITY OF THE Asclepias syriaca L. ROOT EXTRACT.
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Popov, Milena, Grahovac, Mila, Loc, Marta, Prvulović, Dejan, Budakov, Dragana, Konstantinović, Bojan, Samardžić, Nataša, and Stojanović, Tijana
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ALTERNARIA alternata ,PHYTOPATHOGENIC fungi ,ANTI-infective agents ,DISEASE resistance of plants ,SEED production (Botany) ,PLANT development - Abstract
Asclepias syriaca L. is an invasive plant in Serbia which threatens the biodiversity and ecosystem functionality with its high production of wind-dispersed seeds and the rapid proliferation by the lateral rhizomes. The mechanism that allows its competitiveness is allelopathy - the release of the chemical compounds identified in the roots and leaves which have negative effects on the other plants. These allelochemicals not only affect the development of the neighboring plants, but also are a potential source of the antioxidant and antimicrobial compounds, which increase the immunity of the plant itself, protecting it from the pests and the pathogens. The present study aimed to screen the antifungal activity of the A. syriaca water and methanol extracts. In vitro antimicrobial activity was analyzed by the radial growth assays against the three phytopathogenic fungi isolates: Alternaria alternata, Fusarium avenaceum and Discula platani. The results were processed by factorial ANOVA and the statistically significant differences were determined by Duncan's multiple range test using the software STATISTICA 13.5. The obtained results suggest that the A. syriaca water extract has a significant fungistatic and potential fungicidal effect towards the tested phytopathogenic fungi and thus can be considered as a potential tool for their biological control. [ABSTRACT FROM AUTHOR]
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- 2023
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35. Podosphaera leucotricha (ELLIS ET EVERHART) SALMON - PROUZROKOVAČ PEPELNICE JABUKE.
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Dudaš, Tatjana, Vukotić, Jelena, Loc, Marta, Petreš, Mladen, Budakov, Dragana, Grahovac, Mila, and Stojšin, Vera
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- 2022
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36. PROUZROKOVAČ ČAĐAVE PEGAVOSTI LISTA I KRASTAVOSTI PLODA JABUKE (VENTURIA INAEQUALIS (COOKE) WINTER).
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Petreš, Mladen, Grahovac, Mila, Budakov, Dragana, Stojšin, Vera, Loc, Marta, Dudaš, Tatjana, and Došen, Nataša
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- 2022
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37. Rhizoctonia root rot (Rhizoctoni solani K ü h n) of sugar beet in province Vojvodina
- Author
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Stojšin Vera B., Bagi Ferenc F., Jasnić Stevan M., Balaž Ferenc F., and Budakov Dragana B.
- Subjects
distribution ,aetiology ,Rhizoctonia root rot ,Rhizoctonia solani ,sugar beet ,Vojvodina ,Science (General) ,Q1-390 - Abstract
Sugar beet root rot appears regularly each year, but its intensity depends on agro ecological conditions. The predominant causers of root rot in Vojvodina are fungi from Fusarium genus and species Macrophomina phaseolina. Over the last couple of years, more intense occurrence of Rhizoctonia root rot has been observed. Rhizoctonia solani, the causal agent of root rot is present in sugar beet fields. During 2000-2005, on the territory of Vojvodina, the frequency of Rhizoctonia solani in phytopathological isolations from rotted sugar beet roots was between 0,0-18,2%. The intensity of the disease depends on localities, agro ecological conditions and genotypes. Symptoms of Rhizoctonia root rot were registered at some localities in all regions of Vojvodina: Srem, Banat and Bačka. The disease appearance is above all local. It occurs in small patches, on heavy, non-structured soil and on depressed wet parts of plots. Individual diseased plants can be found during July. Brown rot appears on sugar beet roots, with dried tissue on surface, which is present on the tail as well as on the middle part and the head of root. Tissues with described symptoms are deeper regarding the healthy part of root. On vertical root section, the necrotic changes are clearly visible comparing to tissue section without symptoms. The heavily infected tissue forms fissures on roots in most cases. Besides the above-mentioned symptoms on roots, the plant wilting and leaf handle necrosis as well as leaf dying are also observed. When rot spreads to the whole root head, plants quickly die.
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- 2006
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38. Distribution, Genetic Diversity and Biocontrol of Aflatoxigenic Aspergillus flavus in Serbian Maize Fields
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Vlajkov, Vanja, primary, Grahovac, Mila, additional, Budakov, Dragana, additional, Loc, Marta, additional, Pajčin, Ivana, additional, Milić, Dragan, additional, Novaković, Tihomir, additional, and Grahovac, Jovana, additional
- Published
- 2021
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39. Patogenost Pectobacterium spp. na krtolama različitih sorti krompira
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Loc, Marta, Milošević, Dragana, Ignjatov, Maja, Petreš, Mladen, Budakov, Dragana, Stojšin, Vera, and Grahovac, Mila
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tubers ,cultivars ,Vojvodina ,sorte ,potatoes ,krtola ,Pectobacterium spp ,Serbia ,krompir - Abstract
Vrste Pectobacterium carotovorum subsp. brasiliense (Pcb) i Pectobacterium carotovorum subsp. carotovorum (Pcc) su tokom 2018. i 2019. godine na terioriji AP Vojvodine registrovane kao dominanatni prouzrokovači crne truleži prizemnog dela stabla i vlažne truleži krtola krompira. U postupku detekcije i identifikacije ovih prouzrokovača na simptomatičnim biljakama krompira tokom 2018. i 2019. godine, sekvenciranjem 16S rRNK regiona je za čak 90% dobijenih izolata potvrđena pripadnost vrsti Pcb. Godišnji gubici prinosa i kvaliteta krompira, usled delovanja ovih vrsta poprimaju sve veće razmere, te se one svrstavaju među deset ekonomski najznačajnijih fitopatogenih bakterija. Pored šteta koje nastaju u toku vegetacije, za vreme čuvanja krtola krompira u skladištu pa do njegove upotrebe, patogeni nastavljaju svoju aktivnost, te celokupne partije uskladištenih krtola mogu veoma brzo propasti. Najznačajniji izvor infekcije predstavljaju zaražene semenske krtole, te patogeni ovim putem imaju mogućnost lakog dospevanja u nova područja.
- Published
- 2021
40. Uticaj etarskih ulja na Colletotrichum spp. – prouzrokovače gorke truleži jabuke
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Petreš, Mladen, Aćimović, Milica, Tešević, Vele, Loc, Marta, Stojšin, Vera, Budakov, Dragana, and Grahovac, Mila
- Subjects
fitopatogene gljive ,Colletotrichum acutatum ,etarska ulja ,jabuka ,skladištenje ,storing ,phytopathogenic fungi ,Colletotrichum gloeosporioides ,essential oils ,apples - Abstract
Fitopatogene gljive značajno ugrožavaju kvalitet plodova jabuke tokom skladištenja. Vrste Colletotrichum acutatum i Colletotrichum gloeosporioides, prouzrokovači gorke truleži (antraknoze) plodova jabuke, u pojedinim godinama prouzrokuju značajne gubitke. Colletotrichum spp. zarazu plodova jabuke ostvaruju pre berbe plodova, ali je zaraza moguća i posle ovog perioda. Zaštita plodova jabuke od prouzrokovača truleži se u najvećoj meri oslanja na primenu sintetičkih fungicida, pre berbe plodova. Zbog nepovoljnih ekotoksikoloških svojstava i pojave rezistentnosti, primena hemijskih fungicida tokom vegetacionog perioda se sve više redukuje, dok je njihova upotreba posle berbe plodova zabranjena u Evropskoj uniji, ali i u Srbiji. Imajući u vidu navedeno, kao i činjenicu da su zahtevi potrošača za zdravstveno bezbednom hranom sve izraženiji, istraživanja o alternativnim načinima borbe protiv patogena uskladištenih plodova voća sve više dobijaju na značaju. Etarska ulja pojedinih biljaka ispoljavaju snažno antifungalno dejstvo, te mogu biti potencijalni agensi zaštite plodova jabuke od prouzrokovača truleži.
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- 2021
41. Etarska ulja i hidrolati kao inhibitori razvoja prouzrokovača fuzariozne truleži jabuke
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Petreš, Mladen, Petreš, Mladen, Aćimović, Milica, Loc, Marta, Dudaš, Tatjana, Jovanović, Dimitrije, Budakov, Dragana, Stojšin, Vera, Grahovac, Mila, Petreš, Mladen, Petreš, Mladen, Aćimović, Milica, Loc, Marta, Dudaš, Tatjana, Jovanović, Dimitrije, Budakov, Dragana, Stojšin, Vera, and Grahovac, Mila
- Abstract
Pripadnici roda Fusarium, kao prouzrokovači fuzariozne truleži ploda jabuke, pored gubitaka koje prouzrokuju u proizvodnji jabuke, mogu sintetisati mikotoksine što je dodatni aspekt njihovog štetnog delovanja koji se ogleda u kontaminaciji plodova jabuke i proizvoda koji se dobijaju njenom preradom. Vrsta Fusarium avenaceum je u periodu 2016 - 2020. godine bila dominantni prouzrokovač fuzariozne truleži uskladištenih plodova jabuke na teritoriji R. Srbije, dok se vrsta Fusarium graminearum javljala sporadično. Za obe vrste utvrđen je kapacitet za proizvodnju mikotoksina.
- Published
- 2021
42. Trichoderma harzianumas a potential biological agent in control of the charcoal root rot of sugar beet
- Author
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Stankov Petreš, Aleksandra, Stojšin, Vera, Nagl, Nevena, Petreš, Mladen, Grahovac, Mila, Ćurčić, Živko, and Budakov, Dragana
- Abstract
Sugar beet is one of the most profitable crops in Serbia. The charcoal root rot is an emerging disease of sugar beet that significantly reduces yield and affects the economic viability of production. The objectives of this research were to determine the antagonist activity of selected Trichoderma harzianumisolates (T1 to T13) on two Macrophomina phaseolinaisolates in vitro using a dual culture test and a volatile compounds test, as well as in vivo. The efficacy of these treatments was compared to two commercial biopesticides (Bacillomix® Original and Trifender Pro®) and one synthetic fungicide (Funomil 700 WG®). The strongest inhibition in the dual culture test was observed in the treatment with T. harzianumT2 against both tested M. phaseolinaisolates. Also, the volatile compounds produced by isolates T2 and T12 exhibited the strongest inhibitory effect on M. phaseolinaisolates (from 48.22 to 62.75%). Differences in efficacy were found not only between tested Trichodermaisolates, but also between M. phaseolinaisolates used for inoculation which indicates different susceptibility of pathogen population to bioagent. The in vivo test confirmed the strong antagonistic effect of the T. harzianumT2 and T12 isolates. Bacillomix® Original, Trifender Pro® and Funomil® 700 WG did not express satisfactory effects in pathogen control.
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- 2023
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43. Biological Control of Aflatoxin in Maize Grown in Serbia
- Author
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Savić, Zagorka, Savić, Zagorka, Dudaš, Tatjana, Loc, Marta, Grahovac, Mila, Budakov, Dragana, Jajić, Igor, Krstović, Saša, Barošević, Tijana, Krska, Rudolf, Sulyok, Michael, Stojšin, Vera, Petreš, Mladen, Stankov, Aleksandra, Vukotić, Jelena, Bagi, Ferenc, Savić, Zagorka, Savić, Zagorka, Dudaš, Tatjana, Loc, Marta, Grahovac, Mila, Budakov, Dragana, Jajić, Igor, Krstović, Saša, Barošević, Tijana, Krska, Rudolf, Sulyok, Michael, Stojšin, Vera, Petreš, Mladen, Stankov, Aleksandra, Vukotić, Jelena, and Bagi, Ferenc
- Abstract
Aspergillus flavus is the main producer of aflatoxin B1, one of the most toxic contaminants of food and feed. With global warming, climate conditions have become favourable for aflatoxin contamination of agricultural products in several European countries, including Serbia. The infection of maize with A. flavus, and aflatoxin synthesis can be controlled and reduced by application of a biocontrol product based on non‐toxigenic strains of A. flavus. Biological control relies on competition between atoxigenic and toxigenic strains. This is the most commonly used biological control mechanism of aflatoxin contamination in maize in countries where aflatoxins pose a significant threat. Mytoolbox Af01, a native atoxigenic A. flavus strain, was obtained from maize grown in Serbia and used to produce a biocontrol product that was applied in irrigated and non‐irrigated Serbian fields during 2016 and 2017. The application of this biocontrol product reduced aflatoxin levels in maize kernels (51–83%). The biocontrol treatment had a highly significant effect of reducing total aflatoxin contamination by 73%. This study showed that aflatoxin contamination control in Serbian maize can be achieved through biological control methods using atoxigenic A. flavus strains.
- Published
- 2020
44. Characterisation of Trichoderma spp. for antagonistic activity against charcoal root rot Macrophomina phaseolina from sugar beet
- Author
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Stankov, Aleksandra, Stankov, Aleksandra, Nagl, Nevena, Stojšin, Vera, Budakov, Dragana, Bagi, Ferenc, Dudaš, Tatjana, Ćurčić, Živko, Isakov, Milada, Stankov, Aleksandra, Stankov, Aleksandra, Nagl, Nevena, Stojšin, Vera, Budakov, Dragana, Bagi, Ferenc, Dudaš, Tatjana, Ćurčić, Živko, and Isakov, Milada
- Abstract
Charcoal rot of sugar beet, caused by Macrophomina phaseolina in recent years has become a main concern for farmers in Serbia. Since control measures are mostly preventive with only partial effect, introduction of alternative measures, such as biological control based on augmentation of mycoparasites, represents the most promising approach. ITS region and tef gene sequence analysis of ten Trichoderma spp. isolates from sugar beet and soybean rhizosphere was done to confirm species identity. All isolates were confirmed to be Trichoderma harzianum.
- Published
- 2020
45. Effect of different sowing dates on Cercospora beticola infection level
- Author
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Ćurčić, Živko, Ćurčić, Živko, Budakov, Dragana, Stankov, Aleksandra, Taški-Ajduković, Ksenija, Nagl, Nevena, Stojšin, Vera, Ćurčić, Živko, Ćurčić, Živko, Budakov, Dragana, Stankov, Aleksandra, Taški-Ajduković, Ksenija, Nagl, Nevena, and Stojšin, Vera
- Abstract
Cercospora leaf spot caused by the fungus Cercospora beticola is the most significant foliar disease of sugar beet in Serbia. Measures to reduce damage of this disease are based on the use of tolerant varieties, crop rotation and fungicide application. Climate changes and the resistance of Cercospora beticolastrains to fungicides from the strobilurin group further complicate the fight against this disease. In extremely favourable years for the development of disease with high temperatures and precipitation during the summer months, growers in Serbia perform 4-8 fungicide treatments.
- Published
- 2020
46. Influence of NPK mineral nutrition and cultivar on sugar beet root rot
- Author
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Stojšin, Vera, Stojšin, Vera, Budakov, Dragana, Ćurčić, Živko, Stankov, Aleksandra, Dudaš, Tatjana, Bagi, Ferenc, Nagl, Nevena, Stojšin, Vera, Stojšin, Vera, Budakov, Dragana, Ćurčić, Živko, Stankov, Aleksandra, Dudaš, Tatjana, Bagi, Ferenc, and Nagl, Nevena
- Abstract
Sugar beet is susceptible to a number of root rot diseases, and they are regularly occurring in Serbia. Economically most important pathogens are Macrophomina phaseolina and Fusarium spp.. Macrophomina phaseolina is predominantly found in isolates from sugar beet, especially during dry and hot summers. For more than thirty years already, our team of researchers follows the occurrence and etiology of sugar beet root rot in Serbia. The aim of this experiment was to evaluate the effect of mineral nutrition on root rot in commercial sugar beet cultivars.
- Published
- 2020
47. Pectobacterium and Dickeya species causing potato blackleg and tuber soft rot
- Author
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Loc, Marta, Loc, Marta, Petrović, Gordana, Ignjatov, Maja, Budakov, Dragana, Petreš, Mladen, Stojšin, Vera, Grahovac, Mila, Loc, Marta, Loc, Marta, Petrović, Gordana, Ignjatov, Maja, Budakov, Dragana, Petreš, Mladen, Stojšin, Vera, and Grahovac, Mila
- Abstract
World potato production is affected by plant pathogenic bacteria, which significantly limit crop yield and quality in recent years. Bacteria belonging to Pectobacterium and Dickeya genera, causal agents of blackleg and tuber soft rot of potato, are considered as one of the most important pathogens that affect plant production worldwide. Main source of infection are latently infected seed tubers, which transmitted over long trade distances significantly increase disease incidence. Main identification tools for Pectobacterium and Dickeya species until recently were morphological and biochemical characterization methods, followed by pathogenicity tests. Due to phylogenetic heterogeneity of the soft rot and blackleg causing bacteria, identification and differentiation to species and subspecies level became increasingly difficult. Therefore, development of efficient and reliable identification methods is of high importance. Molecular and serological methods offer accurate, reliable and cost-effective differentiation of high specificity and reproducibility. This work aimed to review Pectobacterium i Dickeya species occurring on potato, with special highlight on available molecular identification techniques., Proizvodnja krompira u svetu značajno je ugrožena delovanjem fitopatogenih bakterija, te godišnji gubici prinosa poprimaju sve veće razmere. Među deset ekonomski najznačajnijih fitopatogenih bakterija koje smanjuju prinos i kvalitet poljoprivrednih kultura ističu se vrste rodova Pectobacterium i Dickeya - prouzrokovači crne truleži prizemnog dela stabla "crna noga" i vlažne truleži krtola krompira. Najznačajniji izvor infekcije su zaražene semenske krtole, te bakterije ovim putem lako dospevaju u nova područja. Identifikacija bakterija rodova Pectobacterium i Dickeya se do nedavno zasnivala na proučavanju morfoloških odlika bakterijskih kolonija uz upotpunjavanje testovima patogenosti i biohemijskim analizama. Zbog heterogenosti populacije patogena precizna identifikacija prouzrokovača vlažne truleži je dodatno otežana. S obzirom na potrebe za brzom i pouzdanom identifikacijom, u primenu su uvedene serološke i molekularne metode. Molekularne tehnike identifikacije, zbog svoje visoke specifičnosti, brzine izvođenja, pouzdanosti i ponovljivosti danas zauzimaju vodeće mesto u identifikaciji patogena. U radu su prikazane vrste rodova Pectobacterium i Dickeya koje se javljaju na krompiru, sa posebnim osvrtom na molekularne tehnike identifikacije.
- Published
- 2020
48. Pseudomycosis and mycosis of rocket plant (arugula)
- Author
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Panić, Milica, primary, Stojšin, Vera, additional, Budakov, Dragana, additional, and Grahovac, Mila, additional
- Published
- 2021
- Full Text
- View/download PDF
49. Effects of Bacillus velezensis on Fusarium avenaceum, a causal agent of post-harvest apple fruit rot
- Author
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Petres, Mladen, primary, Loc, Marta, primary, Grahovac, Mila, primary, Stojsin, Vera, primary, Budakov, Dragana, primary, Grahovac, Jovana, primary, Pajčin, Ivana, primary, and Vlajkov, Vanja, primary
- Published
- 2020
- Full Text
- View/download PDF
50. The susceptibility of different potato cultivars to blackleg and soft rot caused by Pectobacterium spp.
- Author
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Loc, Marta, primary, Milošević, Dragana, primary, Ignjatov, Maja, primary, Budakov, Dragana, primary, Petres, Mladen, primary, Stojsin, Vera, primary, and Grahovac, Mila, primary
- Published
- 2020
- Full Text
- View/download PDF
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