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O Pennisetum purpureum (Schumach) cultivar BRS Capiaçu destaca-se das demais espécies forrageiras por apresentar elevada capacidade produtiva. Visando melhorar o manejo vegetal a campo, é necessário uniformizar a germinação/emergência de sementes ou mudas, bem como o rápido desenvolvimento vegetal. Assim, a utilização de bactérias promotoras de crescimento de planta enquadra-se como uma estratégia viável e sustentável, sobretudo por reduzir diversos danos ambientais causados pela prática trivial de diversos insumos agrícolas. Este estudo objetivou avaliar a germinação, as características morfológicas e fisiológicas de plântulas de BRS Capiaçu, após a inoculação do colmo/mudas com bactérias promotoras de crescimento de plantas isoladas de Brachiaria decumbens Stapf. e Brachiaria humidicola (Rendle.) Schweickerdt. O experimento foi realizado em delineamento inteiramente casualizado, com duas avaliações, ao 11º e 25º dias após as inoculações. Foram avaliados 19 tratamentos, sendo 16 inoculantes bacterianos, dois inoculantes comerciais (Biomais® e Biomaphos®) e um controle sem inoculação. Todos os tratamentos foram mantidos em câmara de germinação a 25°C, desvio padrão ±5°C sob 12h de fotoperíodo. As inoculações bacterianas promoveram aumentos na germinação e nas características morfológicas e fisiológicas de plântulas de BRS Capiaçu no 11º e 25º dias após suas inoculações, superando o tratamento controle e, por vezes, assemelhando ou superando os inóculos comerciais. As bactérias isoladas de B. decumbens e com alta produção de ácido indolacético proporcionaram os maiores incrementos no desenvolvimento das plântulas. Por fim, conclui-se que os inóculos bacterianos, além de melhorar o estabelecimento de plantas de Capiaçu, são uma alternativa eficaz e sustentável ao uso de produtos sintéticos, contribuindo com um ecossistema agrícola ambientalmente mais equilibrado.

Abstract Objective In Brazil, postpartum hemorrhage (PPH) is a major cause of maternal morbidity and mortality. Data on the profile of women and risk factors associated with PPH are sparse. This study aimed to describe the profile and management of patients with PPH, and the association of risk factors for PPH with severe maternal outcomes (SMO). Methods A cross-sectional study was conducted in Instituto de Medicina Integral Prof. Fernando Figueira (IMIP) obstetric intensive care unit (ICU) between January 2012 and March 2020, including patients who gave birth at the hospital and that were admitted with PPH to the ICU. Results The study included 358 patients, of whom 245 (68.4%) delivered in the IMIP maternity, and 113 (31.6%) in other maternity. The mean age of the patients was 26.7 years, with up to eight years of education (46.1%) and a mean of six prenatal care. Uterine atony (72.9%) was the most common cause, 1.6% estimated blood loss, 2% calculated shock index (SI), 63.9% of patients received hemotransfusion, and 27% underwent hysterectomy. 136 cases of SMO were identified, 35.5% were classified as maternal near miss and 3.0% maternal deaths. Multiparity was associated with SMO as an antepartum risk factor (RR=1.83, 95% CI1.42-2.36). Regarding intrapartum risk factors, abruptio placentae abruption was associated with SMO (RR=2.2 95% CI1.75-2.81). Among those who had hypertension (49.6%) there was a lower risk of developing SMO. Conclusion The principal factors associated with poor maternal outcome were being multiparous and placental abruption.

Os microrganismos desempenham papel crucial quando associados mutuamente às plantas e podem ser considerados uma nova ferramenta sustentável para a proteção e promoção do crescimento de pastagens. Desta forma, o objetivo deste trabalho foi bioprospectar um consórcio microbiano com habilidades para a promoção de crescimento e desenvolvimento de pastagens de U. decumbens cv. Basilisk, com base na hipótese de que consórcios microbianos podem apresentar potencial semelhante à adubação química. Portanto, foram selecionados cinco consórcios microbianos (MIX 1; 2; 3; 4 e 5), previamente descritos, taxonomicamente e biotecnologicamente. Para atingir os objetivos alcançados foram 7 tratamentos, sendo 5 tratamentos com MIX's, um tratamento com adubação química e um tratamento testemunha sem co-inoculação e sem adubação química . Foi possível observar que de forma geral, os consórcios microbianos apresentaram o potencial de incremento no crescimento das pastagens, para teor de clorofila, número de folhas, número de perfilhos, comprimento da raiz, massa verde e seca da raiz mais que o tratamento testemunha, e mesmo potencial no tratamento com adubação química, para essas características mencionadas. Especificamente, o MIX 1 composto por bactérias Kleibsiela sp., Rhizobium sp. e Sinomonas sp., apresentaram elevado potencial de incremento, superando o tratamento com adubação química, principalmente nas variáveis ​​comprimento, massa verde e seca da raiz. Desta forma, pode-se sugerir que os consórcios microbianos podem se tornar uma alternativa viável ecologicamente, socialmente e economicamente para a manutenção de pastagens.

Davi Kopenawa, um xamã e porta-voz Yanomami contemporâneo, acredita que, se os brancos, que vivem em um mundo ignorante, conseguirem ouvir suas palavras, talvez, possam ajudar uns aos outros a evitar ou, ainda que seja, adiar a queda do céu. Walter Benjamin afirma que a arte de narrar está em vias de extinção no ensaio O Contador de Histórias de 1936. Para Benjamin, a arte de narrar desfaleceu na medida em que se deu o período moderno em uma analogia com a consolidação do capitalismo. Nada mais justo evocar o provérbio Se conselho fosse bom, ninguém dava, vendia. Porém, Davi Kopenawa parece ir contra a tal ordem capitalista ao propor outra visão de mundo com suas narrativas cosmogônicas. Nesse sentido, este artigo pretende confrontar a figura do contador de histórias indígena ao contador de histórias moderno, proposto por Benjamin considerando não somente a arte de narrar, como também, de dar conselhos. Indo além, proponho uma reflexão sobre as palavras de Davi Kopenawa ao lançar a seguinte proposição: como Davi Kopenawa, sendo um contador de histórias, dá conselhos, ou senão, ensina a arte de narrar a Walter Benjamin? Para tal, este artigo fará uma articulação entre a textualidade indígena Yanomami e o ensaio benjaminiano para a construção de uma Teoria Literária Ameríndia como um convite para a escuta imaginativa de outros mundos possíveis.

Objetivo: Compreender as causas que levam a não adesão dos profissionais de enfermagem às capacitações realizadas no hospital. Métodos: Estudo de caráter exploratório-descritivo, do tipo transversal e de cunho qualitativo, realizado com profissionais de enfermagem de uma clínica de hospital de Belém-Pa por meio de questionários. Resultados: O total da população deste estudo é composta de 25 funcionários: 5 enfermeiros, 11 técnicos de enfermagem, 8 auxiliares de enfermagem. Após a análise dos questionários, identificou-se 3 categorias: Conclusão: Os resultados demonstram a necessidade de se adaptar as práticas da Educação Permanente em Saúde a realidade do profissional e da instituição. É importante salientar que apesar das dificuldades enfrentadas pelos profissionais, eles compreendem a importância da Educação Permanente, isso demostra relevância deste estudo, pois percebendo o porquê das causas da não adesão pode-se trabalhar em medidas que reduzam a dispersão do profissional de enfermagem nas atividades educativas no local de trabalho.

Introdução: O procedimento de tatuagem oferece risco biológico aos profissionais e clientes. Objetivo: Descrever as condições higiênicas e sanitárias, bem como analisar a adoção de procedimentos para a gestão de riscos de biossegurança por profissionais tatuadores que atuam em estúdios de tatuagem de um município localizado na região noroeste do estado do Ceará, Brasil. Método: Estudo qualitativo, descritivo e transversal, realizado em cinco estúdios de tatuagem através da aplicação de um checklist baseado em Referência Técnica para o funcionamento dos serviços de tatuagem e piercing da Agência Nacional de Vigilância Sanitária (2009). Resultados: Os principais pontos negativos fazem alusão ao funcionamento de estúdios clandestinos, registro precário dos dados pessoais dos clientes e coleta inadequada do lixo infectante. Por outro lado, os principais pontos positivos alcançados nesse estudo estão relacionados com o descarte adequado de objetos perfurocortantes, existência de local reservado e adequado para o procedimento de tatuagem, incluindo pia para a lavagem das mãos próxima ao local do procedimento. A pesquisa evidenciou também que os profissionais tatuadores possuem materiais e equipamentos apropriados para as respectivas práticas, porém executam procedimentos de biossegurança de maneira incorreta, os quais predispõem profissionais e clientes a fatores de risco biológico. Conclusões: Os estúdios de tatuagem analisados apresentam pobre adequação às normas da Vigilância Sanitária, as quais são insuficientes para garantir a promoção e manutenção da segurança para a saúde de todos os envolvidos em procedimentos de estética corporal do tipo tatuagem.

Lignin is a recalcitrant macromolecule formed by three alcohols (monolignols) predominantly connected by β-aryl ether linkages and is one of the most abundant organic macromolecules in the biosphere. However, the role played by environmental bacteria in lignin degradation is still not entirely understood. In this study, we identified an environmental Klebsiella strain isolated from sediment collected from an altitudinal region in a unique Brazilian biome called Caatinga. This organism can also grow in the presence of kraft lignin as a sole source of carbon and aromatic compounds. We performed whole-genome sequencing and conducted an extensive genome-based metabolic reconstruction to reveal the potential mechanisms used by the bacterium Klebsiella variicola P1CD1 for lignin utilization as a carbon source. We identified 262 genes associated with lignin-modifying enzymes (LMEs) and lignin-degrading auxiliary enzymes (LDAs) required for lignin and aromatic compound degradation. The presence of one DyP (Dye-decolorizing Peroxidase) gene suggests the ability of P1CD1 strain to access phenolic and nonphenolic structures of lignin molecules, resulting in the production of catechol and protocatechuate (via vanillin or syringate) along the peripheral pathways of lignin degradation. K. variicola P1CD1 uses aldehyde-alcohol dehydrogenase to perform direct conversion of vanillin to protocatechol. The upper funneling pathways are linked to the central pathways of the protocatechuate/catechol catabolic branches via β-ketoadipate pathways, connecting the more abundant catabolized aromatic compounds with essential cellular functions, such as energy cellular and biomass production (i.e., via acetyl-CoA formation). The combination of phenotypic and genomic approaches revealed the potential dissimilatory and assimilatory ability of K. variicola P1CD1 to perform base-catalyzed lignin degradation, acting on high- and low-molecular-weight lignin fragments. These findings will be relevant for developing metabolic models to predict the ligninolytic mechanism used by environmental bacteria and shedding light on the flux of carbon in the soil.

Bacteria with intrinsic resistance to antibiotics are found in nature. Such organisms may acquire additional resistance genes from bacteria introduced into soil or water, and the resident bacteria may be the reservoir or source of widespread resistant organisms found in many environments. We isolated antibiotic-resistant bacteria in freshwater samples from 16 U.S. rivers at 22 sites and measured the prevalence of organisms resistant to β-lactam and non β-lactam antibiotics. Over 40% of the bacteria resistant to more than one antibiotic had at least one plasmid. Ampicillin resistance genes, as well as other resistance traits, were identified in 70% of the plasmids. The most common resistant organisms belonged to the following genera: Acinetobacter, Alcaligenes, Citrobacter, Enterobacter, Pseudomonas, and Serratia.

Aim: To investigate the efficacy and tolerability of a cream (Rilastil Xerolact PB) containing a mixture of prebiotics and postbiotics, and to validate the PRURISCORE itch scale in the management of atopic dermatitis. Methods: The study is based on 396 subjects of both sexes in three age groups (i.e., infants, children, adults) suffering from mild/moderate Atopic Dermatitis, recruited from 8 European countries and followed for 3 months. Results: The product demonstrated good efficacy combined with good/very good tolerability in all age groups. In particular, SCORAD, PRURISCORE and IGA scores decreased significantly over the course of the study. The PRURISCORE was preferred to VAS by the vast majority of patients. Conclusion: Even though the role of prebiotics and postbiotics was not formally demonstrated since these substances were part of a complex formulation, it can be reasonably stated that prebiotics and postbiotics have safety and standardization features that probiotics do not have. In addition they are authorized by regulatory authorities, whereas topical probiotics are not.

Background: Delayed puberty is a possible complication of Epidermolysis Bullosa (EB), though the actual incidence is still unknown. In chronic illnesses delayed puberty should be correctly managed since, if untreated, can have detrimental effects on adult height attainment, peak bone mass achievement and psychological health., Aims and Methods: This is a single-centre study on pubertal development, growth and bone status in EB. Auxological, densitometric (areal Bone Mineral Density-aBMD Z-score, Bone Mineral Apparent Density-BMAD Z-score, Trabecular Bone Score-TBS and Bone Strain Index-BSI at Lumbar spine) and body composition data (Total Body DXA scans) were collected. Disease severity was defined according to Birmingham Epidermolysis Bullosa Severity (BEBS) score., Results: Twenty-one patients (12 Recessive Dystrophic EB-RDEB, 3 Dominant Dystrophic EB, 3 Junctional EB-JEB, 2 EB Simplex and one Kindler EB) aged 13 years (females) or 14 years (males) and above were enrolled (age 16.2±2.5 years, M/F 11/10). Short stature was highly prevalent (57%, mean height -2.12±2.05 SDS) with 55% patients with height <-2SD their mid-parental height. 7/21 patients (33%, 6 RDEB and 1 JEB) had delayed puberty with a median BEBS of 50 (range 29 to 63), a height SDS of -2.59 SDS (range -5.95 to -2.22) and a median lumbar BMAD Z-score of -4.0 SDS (range -5.42 to -0.63 SDS). Pubertal status was negatively associated with BEBS, skin involvement, inflammatory state and positively with height SDS and BMI SDS., Conclusions: Pubertal delay is highly prevalent in EB, especially in patients with RDEB and JEB, high severity score and inflammatory state. Moreover, pubertal delay worsens growth impairment and bone health. A study on pubertal induction is ongoing to enlighten possible beneficial effects on adult height attainment and peak bone mass accrual., Competing Interests: The authors have no competing interest to declare.

Background: Living with a rare disease has profound effects on the patient's life and that of their entire family, with practical and psychosocial consequences. This is particularly true when the patient is a child. The principal aim of this study was to measure the family burden in Epidermolysis Bullosa (EB). The secondary endpoint was to evaluate the possible correlation between family burden and the severity of EB., Methods: A sample of 50 families with one or two children affected by EB were recruited between January 2016 and February 2017 to answer a 20-item questionnaire - the EB Burden of Disease (EB-BoD) - developed and validated to assess the family burden of children with EB., Results: The presence of a child with EB may have profound negative implications on several different areas of daily life. In particular, the results demonstrate important differences between the different subtypes of epidermolysis bullosa regarding most of the categories considered by the questionnaire. For three categories out of four (family life, child's life, economic and social impact), a higher score is observed for children with the more debilitating forms of EB: recessive dystrophic EB (RDEB) and junctional EB (JEB)., Conclusions: It is important to work with patients and their families to identify and strengthen adaptive and coping behaviors. That is possible only through the synergistic working of a multidisciplinary team made up of experienced doctors, psychologists, and social workers while in contact with patient Associations.

Background: Harlequin ichthyosis (HI) is the most severe phenotype of autosomal recessive congenital ichthyosis. Juvenile Idiopathic Arthritis (JIA) represents a heterogenous group of disorders all sharing the clinical manifestation of chronic arthritis. Association of HI and chronic arthritis has been reported in few cases., Case Presentation: We report the case of a child with HI who developed a severe form of chronic polyarthritis during the first years of life, treated with repeated multiple joint injections, methotrexate and etanercept with good response and without any adverse events., Conclusion: The reported case and the literature review highlighted the presence of a peculiar severe seronegative polyarthritis with early onset in a series of patients with HI, suggesting that polyarthritis may be a specific manifestation of HI, rather than a rare combination of two separate conditions.

The autosomal recessive congenital ichthyoses (ARCI) are a nonsyndromic group of cornification disorders that includes lamellar ichthyosis, congenital ichthyosiform erythroderma, and harlequin ichthyosis. To date mutations in ten genes have been identified to cause ARCI: TGM1 , ALOX12B , ALOXE3 , NIPAL4 , CYP4F22 , ABCA12 , PNPLA1 , CERS3 , SDR9C7 , and SULT2B1 . The main focus of this report is the mutational spectrum of the genes ALOX12B and ALOXE3 , which encode the epidermal lipoxygenases arachidonate 12-lipoxygenase, i.e., 12R type (12R-LOX), and the epidermis-type lipoxygenase-3 (eLOX3), respectively. Deficiency of 12R-LOX and eLOX3 disrupts the epidermal barrier function and leads to an abnormal epidermal differentiation. The type and the position of the mutations may influence the ARCI phenotype; most patients present with a mild erythrodermic ichthyosis, and only few individuals show severe erythroderma. To date, 88 pathogenic mutations in ALOX12B and 27 pathogenic mutations in ALOXE3 have been reported in the literature. Here, we presented a large cohort of 224 genetically characterized ARCI patients who carried mutations in these genes. We added 74 novel mutations in ALOX12B and 25 novel mutations in ALOXE3 . We investigated the spectrum of mutations in ALOX12B and ALOXE3 in our cohort and additionally in the published mutations, the distribution of these mutations within the gene and gene domains, and potential hotspots and recurrent mutations.

Hookworm-related cutaneous larva migrans is an infestation of the skin caused by nematodes. Involvement of genitals is extremely rare. We report the case of a child with this infestation on the penis who cleared rapidly with topical ivermectin., (© 2019 Wiley Periodicals, Inc.)

Skin protects its host from its environment and allows their interactions by providing a physical permeability barrier, protection from infectious agents, thermoregulation, and ultraviolet protection. Textiles, in particular clothing, interact with skin functions in a dynamic pattern. For years cotton has been considered as the only comfortable tissue suitable for patients with dermatologic disorders. Nowadays new synthetic fibers with important functions, for example breathability and waterproofing have leaned out and new tissues can be used as a complementary tool in dermatologic treatments. Our purpose is to report the main fibers used for dermatological problems and to review the literature on their use in dermatological field; finally, we also report our personal experience on this topic.