28 results on '"Braud, Laura"'
Search Results
2. Platelets Facilitate the Wound-Healing Capability of Mesenchymal Stem Cells by Mitochondrial Transfer and Metabolic Reprogramming
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Levoux, Jennyfer, Prola, Alexandre, Lafuste, Peggy, Gervais, Marianne, Chevallier, Nathalie, Koumaiha, Zeynab, Kefi, Kaouthar, Braud, Laura, Schmitt, Alain, Yacia, Azzedine, Schirmann, Aurélie, Hersant, Barbara, Sid-Ahmed, Mounia, Ben Larbi, Sabrina, Komrskova, Katerina, Rohlena, Jakub, Relaix, Frederic, Neuzil, Jiri, and Rodriguez, Anne-Marie
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- 2021
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3. Heterozygous RPA2variant as a novel genetic cause of telomere biology disorders
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Kochman, Rima, Ba, Ibrahima, Yates, Mai¨lyn, Pirabakaran, Vithura, Gourmelon, Florian, Churikov, Dmitri, Laffaille, Marc, Kermasson, Lae¨titia, Hamelin, Coline, Marois, Isabelle, Jourquin, Frédéric, Braud, Laura, Bechara, Marianne, Lainey, Elodie, Nunes, Hilario, Breton, Philippe, Penhouet, Morgane, David, Pierre, Géli, Vincent, Lachaud, Christophe, Maréchal, Alexandre, Revy, Patrick, Kannengiesser, Caroline, Saintomé, Carole, and Coulon, Stéphane
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In this study, Kochman et al. identified two unrelated individuals with clinical manifestations of telomere biology disorders and short telomeres associated with identical RPA2 variants that reduce binding to ubiquitin ligase RFWD3, resulting in RPA2 accumulation at telomeres and short and dysfunctional telomeres. This study indicates that the identified variant in RPA2 represents a novel genetic cause of TBD and further supports the fundamental role of the RPA complex in regulating telomere length and stability in humans.
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- 2024
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4. Signaling by CO: Molecular and Cellular Functions
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Foresti, Roberta, primary, Braud, Laura, additional, and Motterlini, Roberto, additional
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- 2018
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5. Induction of telomerase in p21-positive cells counteracts capillaries rarefaction in aging mice lung
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Lipskaia, Larissa, primary, Breau, Marielle, additional, Cayrou, Christelle, additional, Churikov, Dmitri, additional, Braud, Laura, additional, Fouillade, Charles, additional, Curras-Alonso, Sandra, additional, Bauwens, Serge, additional, Jourquin, Frederic, additional, Fiore, Frederic, additional, Castellano, Rémy, additional, Josselin, Emmanuelle, additional, Sánchez-Ferrer, Carlota, additional, Giovinazzo, Giovanna, additional, Gilson, Eric, additional, Flores, Ignacio, additional, Londono-Vallejo, Arturo, additional, Adnot, Serge, additional, and Géli, Vincent, additional
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- 2022
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6. Adipose tissue senescence is mediated by increased ATP content after a short-term high-fat diet exposure
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PINI, MARIA, Czibik, Gabor, Sawaki, Daigo, Mezdari, Zaineb, Braud, Laura, Delmont, Thaïs, Mercedes, Raquel, Martel, Cécile, Buron, Nelly, Marcelin, Geneviève, Borgne‐sanchez, Annie, Foresti, Roberta, Motterlini, Roberto, Henegar, Corneliu, Derumeaux, Geneviève, Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Hôpital Henri Mondor, Mitologics S.A.S., Hôpital Robert Debré Paris, Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Nutrition et obésités: approches systémiques (UMR-S 1269) (Nutriomics), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Foresti, Roberta, and Nutrition et obésités: approches systémiques (nutriomics) (UMR-S 1269 INSERM - Sorbonne Université)
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Male ,Original Paper ,obesity ,[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology ,exercise ,nutritional and metabolic diseases ,food and beverages ,Original Articles ,Diet, High-Fat ,bioenergetics ,adipose tissue senescence ,ATP ,Mice ,Adenosine Triphosphate ,Adipose Tissue ,Animals ,lipids (amino acids, peptides, and proteins) ,Energy Metabolism ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology ,hormones, hormone substitutes, and hormone antagonists ,xanthine oxidase - Abstract
In the context of obesity, senescent cells accumulate in white adipose tissue (WAT). The cellular underpinnings of WAT senescence leading to insulin resistance are not fully elucidated. The objective of the current study was to evaluate the presence of WAT senescence early after initiation of high‐fat diet (HFD, 1–10 weeks) in 5‐month‐old male C57BL/6J mice and the potential role of energy metabolism. We first showed that WAT senescence occurred 2 weeks after HFD as evidenced in whole WAT by increased senescence‐associated ß‐galactosidase activity and cyclin‐dependent kinase inhibitor 1A and 2A expression. WAT senescence affected various WAT cell populations, including preadipocytes, adipose tissue progenitors, and immune cells, together with adipocytes. WAT senescence was associated with higher glycolytic and mitochondrial activity leading to enhanced ATP content in HFD‐derived preadipocytes, as compared with chow diet‐derived preadipocytes. One‐month daily exercise, introduced 5 weeks after HFD, was an effective senostatic strategy, since it reversed WAT cellular senescence, while reducing glycolysis and production of ATP. Interestingly, the beneficial effect of exercise was independent of body weight and fat mass loss. We demonstrated that WAT cellular senescence is one of the earliest events occurring after HFD initiation and is intimately linked to the metabolic state of the cells. Our data uncover a critical role for HFD‐induced elevated ATP as a local danger signal inducing WAT senescence. Exercise exerts beneficial effects on adipose tissue bioenergetics in obesity, reversing cellular senescence, and metabolic abnormalities., High‐fat diet (HFD) induced senescence of various white adipose tissue (WAT) cell types as one of the earliest events preceding mitochondrial dysfunction, inflammation, and fibrosis. In WAT of HFD‐fed mice and preadipocytes derived from HFD‐fed mice, ATP levels were increased. The anti‐senescence effects of exercise were partially dependent on purine catabolism as blocking xanthine oxidase activity increased ATP levels and inhibited exercise's rescue effects in subcutaneous WAT.
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- 2021
7. Increased Sirt1 secreted from visceral white adipose tissue is associated with improved glucose tolerance in obese Nrf2-deficient mice
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Braud, Laura, PINI, MARIA, Stec, Donald, Manin, Sylvie, Derumeaux, Geneviève, Stec, David, Foresti, Roberta, Motterlini, Roberto, Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Vanderbilt University [Nashville], University of Mississippi Medical Center (UMMC), and Motterlini, Roberto
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NF-E2-Related Factor 2 ,Adipose Tissue, White ,[SDV]Life Sciences [q-bio] ,Mice, Obese ,Intra-Abdominal Fat ,White adipose tissue ,Diet, High-Fat ,environment and public health ,Nrf2 ,Mice ,Sirtuin 1 ,Animals ,Obesity ,lcsh:QH301-705.5 ,lcsh:R5-920 ,Glucose metabolism ,respiratory system ,[SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences ,[SDV.SP] Life Sciences [q-bio]/Pharmaceutical sciences ,[SDV] Life Sciences [q-bio] ,Mice, Inbred C57BL ,Glucose ,lcsh:Biology (General) ,Insulin Resistance ,lcsh:Medicine (General) ,hormones, hormone substitutes, and hormone antagonists ,Research Paper - Abstract
Obesity is associated with metabolic dysregulation characterized by insulin resistance and glucose intolerance. Nuclear factor E2-related factor (Nrf2) is a critical regulator of the stress response and Nrf2-deficient mice (Nrf2−/−) are protected against high fat diet (HFD)-induced metabolic derangement. We searched for factors that could underline this favorable phenotype and found that Nrf2−/− mice exhibit higher circulating levels of sirtuin 1 (Sirt1), a key player in cellular homeostasis and energy metabolism, compared to wild-type mice. Increased Sirt1 levels in Nrf2−/− mice were found not only in animals under standard diet but also following HFD. Interestingly, we report here that the visceral adipose tissue (eWAT) is the sole source of increased Sirt1 protein in plasma. eWAT and other fat depots displayed enhanced adipocytes lipolysis, increased fatty acid oxidation and glycolysis, suggesting autocrine and endocrine actions of Sirt1 in this model. We further demonstrate that removal of eWAT completely abolishes the increase in circulating Sirt1 and that this procedure suppresses the beneficial effect of Nrf2 deficiency on glucose tolerance, but not insulin sensitivity, following a HFD regime. Thus, in contrast to many other stressful conditions where Nrf2 deficiency exacerbates damage, our study indicates that up-regulation of Sirt1 levels specifically in the visceral adipose tissue of Nrf2−/− mice is a key adaptive mechanism that mitigates glucose intolerance induced by nutritional stress., Graphical abstract Image 1, Highlights • Nrf2−/− mice are protected against weight gain and metabolic dysfunction under high fat diet (HFD). • Nrf2−/− mice display increased production of sirtuin 1 exclusively in visceral white adipose tissue (eWAT). • Removal of eWAT prior to HFD abolishes the increase in sirtuin 1 and impairs glucose tolerance. • A strong link exists between Nrf2 deficiency, SIRT1 in eWAT and regulation of glucose homeostasis.
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- 2021
8. Telomerase Prevents Emphysema in Old Mice by Sustaining Subpopulations of Endothelial and AT2 Cells
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Breau, Marielle, primary, Cayrou, Christelle, additional, Churikov, Dmitri, additional, Fouillade, Charles, additional, Curras-Alonso, Sandra, additional, Bauwens, Serge, additional, Jourquin, Frederic, additional, Braud, Laura, additional, Fiore, Frederic, additional, Castellano, Remy, additional, Josselin, Emmanuelle, additional, Sanchez-Ferrer, Carlotta, additional, Giovinazzo, Giovanna, additional, Gilson, Eric, additional, Flores, Ignacio, additional, Londono-Vallejo, Arturo, additional, Adnot, Serge, additional, and Geli, Vincent, additional
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- 2021
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9. LIPE-related lipodystrophic syndrome: clinical features and disease modeling using adipose stem cells
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Sollier, Camille, primary, Capel, Emilie, additional, Aguilhon, Caroline, additional, Smirnov, Vasily, additional, Auclair, Martine, additional, Douillard, Claire, additional, Ladsous, Miriam, additional, Defoort-Dhellemmes, Sabine, additional, Gorwood, Jennifer, additional, Braud, Laura, additional, Motterlini, Roberto, additional, Vatier, Camille, additional, Lascols, Olivier, additional, Renard, Eric, additional, Vigouroux, Corinne, additional, and Jéru, Isabelle, additional
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- 2021
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10. Platelets Promote Pro-Angiogenic Activity of Mesenchymal Stem Cells Via Mitochondrial Transfer and Metabolic Reprogramming
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Levoux, Jennyfer, primary, Prola, Alexandre, additional, Lafuste, Peggy, additional, Gervais, Marianne, additional, Braud, Laura, additional, Schirmann, Aurélie, additional, Hersant, Barbara, additional, Sid-Ahmed, Mounia, additional, Ben-Larbi, Sabrina, additional, Komrskova, Katerina, additional, Rohlena, Jakub, additional, Relaix, Frederic, additional, Neuzil, Jiri, additional, and Rodriguez, Anne-Marie, additional
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- 2020
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11. MR (Mineralocorticoid Receptor) Induces Adipose Tissue Senescence and Mitochondrial Dysfunction Leading to Vascular Dysfunction in Obesity
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Lefranc, Clara, Friederich, Malou, Braud, Laura, Palacios-Ramirez, Roberto, Karlsson, Susanne, Boujardine, Nabiha, Motterlini, Roberto, Jaisser, Frederic, Cat, Aurelie Nguyen Dinh, Lefranc, Clara, Friederich, Malou, Braud, Laura, Palacios-Ramirez, Roberto, Karlsson, Susanne, Boujardine, Nabiha, Motterlini, Roberto, Jaisser, Frederic, and Cat, Aurelie Nguyen Dinh
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Adipose tissue (AT) senescence and mitochondrial dysfunction are associated with obesity. Studies in obese patients and animals demonstrate that the MR (mineralocorticoid receptor) contributes to obesity-associated cardiovascular complications through its specific role in AT. However, underlying mechanisms remain unclear. This study aims to elucidate whether MR regulates mitochondrial function in obesity, resulting in AT premature aging and vascular dysfunction. Obese (db/db) and lean (db/+) mice were treated with an MR antagonist or a specific mitochondria-targeted antioxidant. Mitochondrial and vascular functions were determined by respirometry and myography, respectively. Molecular mechanisms were probed by Western immunoblotting and real-time polymerase chain reaction in visceral AT and arteries and focused on senescence markers and redox-sensitive pathways. db/db mice displayed AT senescence with activation of the p53-p21 pathway and decreased SIRT (sirtuin) levels, as well as mitochondrial dysfunction. Furthermore, the beneficial anticontractile effects of perivascular AT were lost in db/db via ROCK (Rho kinase) activation. MR blockade prevented these effects. Thus, MR activation in obesity induces mitochondrial dysfunction and AT senescence and dysfunction, which consequently increases vascular contractility. In conclusion, our study identifies novel mechanistic insights involving MR, adipose mitochondria, and vascular function that may be of importance to develop new therapeutic strategies to limit obesity-associated cardiovascular complications.
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- 2019
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12. Platelet-Rich Plasma Improves the Wound Healing Potential of Mesenchymal Stem Cells through Paracrine and Metabolism Alterations
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Hersant, Barbara, primary, Sid-Ahmed, Mounia, additional, Braud, Laura, additional, Jourdan, Maud, additional, Baba-Amer, Yasmine, additional, Meningaud, Jean-Paul, additional, and Rodriguez, Anne-Marie, additional
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- 2019
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13. Human and murine macrophages exhibit differential metabolic responses to lipopolysaccharide - A divergent role for glycolysis
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Vijayan, Vijith, primary, Pradhan, Pooja, additional, Braud, Laura, additional, Fuchs, Heiko R., additional, Gueler, Faikah, additional, Motterlini, Roberto, additional, Foresti, Roberta, additional, and Immenschuh, Stephan, additional
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- 2019
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14. MR (Mineralocorticoid Receptor) Induces Adipose Tissue Senescence and Mitochondrial Dysfunction Leading to Vascular Dysfunction in Obesity
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Lefranc, Clara, primary, Friederich-Persson, Malou, additional, Braud, Laura, additional, Palacios-Ramirez, Roberto, additional, Karlsson, Susanne, additional, Boujardine, Nabiha, additional, Motterlini, Roberto, additional, Jaisser, Frederic, additional, and Nguyen Dinh Cat, Aurelie, additional
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- 2019
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15. Carbon monoxide–induced metabolic switch in adipocytes improves insulin resistance in obese mice
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Braud, Laura, primary, Pini, Maria, additional, Muchova, Lucie, additional, Manin, Sylvie, additional, Kitagishi, Hiroaki, additional, Sawaki, Daigo, additional, Czibik, Gabor, additional, Ternacle, Julien, additional, Derumeaux, Geneviève, additional, Foresti, Roberta, additional, and Motterlini, Roberto, additional
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- 2018
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16. Vascular endothelial function masks increased sympathetic vasopressor activity in rats with metabolic syndrome
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Battault, Sylvain, primary, Meziat, Cindy, additional, Nascimento, Allesandro, additional, Braud, Laura, additional, Gayrard, Sandrine, additional, Legros, Christian, additional, De Nardi, Frederic, additional, Drai, Jocelyne, additional, Cazorla, Olivier, additional, Thireau, Jérôme, additional, Meyer, Gregory, additional, and Reboul, Cyril, additional
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- 2018
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17. Lipotropic effects of antioxidant molecules of tea (Camellia sinensis)
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Braud, Laura, Processus de Transfert et d'Echanges dans l'Environnement - EA 3819 (PROTEE), Université de Toulon (UTLN), Université de Toulon, Jean-Michel Maixent, Joël-Paul Grillasca, and STAR, ABES
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Lipotropic ,[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition ,Dyslipidemia ,Lipotropes ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,Dyslipidémie - Abstract
Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease in industrializedcountries because being strongly associated with the development of metabolic syndrome and relateddyslipidemia. To date, the mechanisms of pathology remain poorly defined and available therapeutic meanshave moderate efficacy. Epidemiological studies have reported a beneficial effect of tea consumption in thefight against liver disorders and cardiovascular risk factors such as dyslipidemia. However, the mechanismsby which a blend of green tea, oolong tea and Pu-erh tea, Hao Ling tea, reduces fatty liver and dyslipidemiaremain unknown. Therefore, the objective of this thesis was to evaluate the effects and mechanisms of actionof Hao Ling tea on NAFLD and dyslipidemia, through two approaches, one on cellular model and the other onanimal model. Our results show that Hao Ling tea reduces the hepatic lipogenesis in vitro and in vivo and thusattenuates steatosis induced by a high fat-high sucrose diet in a rat model. We observed that this tea improvesthe blood lipid profile by increasing plasma HDL levels. We were also able to highlight that tea ownsantioxidant and hepato-protective properties to counteract an inducer of oxidative stress in vitro and todecrease lipid peroxidation in vivo. Finally, we have shown that the oxidative stress per se resulted in anaccumulation of intracellular lipid in isolated hepatocytes and that the tea, due to its antioxidant properties,prevented this phenomenon. The Hao Ling tea is a good nutritional approach in preventing NAFLD and tomaintain LDL/HDL ratio., La stéatose hépatique non-alcoolique (NAFLD) est l’affection hépatique chronique la plus fréquente à l’heure actuelle dans le monde industrialisé car fortement liée au développement du syndrome métabolique et des dyslipidémies associées. À ce jour, les mécanismes de la pathologie restent mal définis et les moyens thérapeutiques disponibles ont une efficacité modérée. Des études épidémiologiques ont rapporté un effet bénéfique de la consommation de thé pour lutter contre les désordres hépatiques et les facteurs de risque cardiovasculaires tel que la dyslipidémie. Cependant, les mécanismes par lesquels le thé atténue la stéatose hépatique et la dyslipidémie restent méconnus. Par conséquent, l’objectif de ce travail de thèse était d’évaluer les effets et les mécanismes d’action d’un mélange de thés vert, oolong et Pu-erh, le thé Hao Ling, sur la NAFLD et la dyslipidémie, à travers deux approches, l’une sur modèle cellulaire et l’autre sur modèle animal.Les résultats ont permis de mettre en évidence que le thé Hao Ling permettait de diminuer la lipogenèse hépatique in vitro et in vivo et ainsi d’atténuer la stéatose induite par un régime hyperlipidique et riche en saccharose chez le rat Wistar. Nous avons observé que ce thé permettait d’améliorer le profil lipidique sanguin en augmentant le taux de HDL plasmatique. Nous avons également pu mettre en évidence que le thé possédait des propriétés antioxydantes et hépato-protectrices permettant de lutter contre un inducteur de stress oxydant in vitro et de diminuer la peroxydation lipidique in vivo. Enfin, nous avons démontré que le stress oxydant "per se" entraînait une accumulation de lipides intracellulaires sur hépatocytes isolés et que le thé, grâce à ses propriétés antioxydantes, prévenait ce phénomène. Le thé Hao Ling constitue une bonne approche nutritionnelle dans la prévention de la NAFLD et dans le maintien du rapport LDL-Cholestérol/HDLCholestérol.
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- 2015
18. Antioxidant properties of tea blunt ROS-dependent lipogenesis: beneficial effect on hepatic steatosis in a high fat-high sucrose diet NAFLD obese rat model
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Braud, Laura, primary, Battault, Sylvain, additional, Meyer, Grégory, additional, Nascimento, Alessandro, additional, Gaillard, Sandrine, additional, de Sousa, Georges, additional, Rahmani, Roger, additional, Riva, Catherine, additional, Armand, Martine, additional, Maixent, Jean-Michel, additional, and Reboul, Cyril, additional
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- 2017
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19. 0149 : Antioxidant molecules of tea (Camellia sinensis) decrease hepatic lipogenesis and steatosis in a high fat-sucrose diet NAFLD rat model
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Braud, Laura, primary, Battault, Sylvain, additional, Meyer, Grégory, additional, Nascimento, Alessandro, additional, Gaillard, Sandrine, additional, De Sousa, Georges, additional, Rahmani, Roger, additional, Riva, Catherine, additional, Armand, Martine, additional, Reboul, Cyril, additional, and Maixent, Jean-Michel, additional
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- 2016
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20. 0093 : Endothelium masks increased sympathetic vasopressor activity in rats with metabolic syndrome
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Battault, Sylvain, primary, Méziat, Cindy, additional, Nascimento, Alessandro, additional, Braud, Laura, additional, Peyrol, Julien, additional, Gayrard, Sandrine, additional, Drai, Jocelyne, additional, Legros, Christian, additional, De Nardi, Frédéric, additional, Cazorla, Olivier, additional, Thireau, Jérôme, additional, Meyer, Grégory, additional, and Reboul, Cyril, additional
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- 2016
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21. Effect of Brewing Duration on the Antioxidant and Hepatoprotective Abilities of Tea Phenolic and Alkaloid Compounds in a t-BHP Oxidative Stress-Induced Rat Hepatocyte Model
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Braud, Laura, primary, Peyre, Ludovic, additional, de Sousa, Georges, additional, Armand, Martine, additional, Rahmani, Roger, additional, and Maixent, Jean-Michel, additional
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- 2015
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22. 0217 : Antioxidant and protective effects of a pu-erh tea extract (camellia sinensis) on primary cultured rat cells
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Braud, Laura, primary, De Sousa, Georges, additional, Peyre, Ludovic, additional, Zeil, Jean-Marc, additional, Rahmani, Roger, additional, and Maixent, Jean-Michel, additional
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- 2015
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23. 0261 Hao Ling pu-erh tea attenuates lipid accumulation in primary culture rat hepatocytes
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Braud, Laura, primary, Sousa, Georges De, additional, Peyre, Ludovic, additional, Zeil, Jean-Marc, additional, Rahmani, Roger, additional, and Maixent, Jean-Michel, additional
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- 2014
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24. Assimilation of multi-sensor and multi-temporal remote sensing data to monitor vegetation and soil : the Alpilles-ReSeDA project
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Prevot, Laurent, Baret, Frédéric, Chanzy, Andre, Olioso, Albert, Wigneron, Jean-Pierre, Autret, Hervé, Baudin, Francois, Bessemoulin, Pierre, BETHENOD, Olivier, Blamont, D., Blavoux, B., Bonnefond, Jean-Marc, Boubkraoui, S., Bouman, B.A.M., Braud, Laura, Bruguier, N., Calvet, Jean-Christophe, Caselles, V., Chauki, H., Clevers, J.G.P.W., Coll, C., Company, A., Courault, Dominique, Dedieu, Gérard, Degenne, Pascal, Moulin, Sophie, Lagouarde, Jean-Pierre, Unité de bioclimatologie, Institut National de la Recherche Agronomique (INRA), Unité de Science du Sol, Unité de recherches en bioclimatologie, and Centre National d’Etudes Spatiales
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modèle de culture ,bilan énergétique ,suivi de cultures ,prévision de rendement ,télédétection ,[SDE.MCG]Environmental Sciences/Global Changes ,Signal and Image processing ,Traitement du signal et de l'image ,bilan hydrique ,Milieux et Changements globaux ,[SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing ,sol agricole ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
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- 1998
25. Effect of Brewing Duration on the Antioxidant and Hepatoprotective Abilities of Tea Phenolic and Alkaloid Compounds in a t-BHP Oxidative Stress-Induced Rat Hepatocyte Model
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Martine Armand, Jean-Michel Maixent, Laura Braud, Roger Rahmani, Georges de Sousa, Ludovic Peyre, Processus de Transfert et d'Echanges dans l'Environnement - EA 3819 (PROTEE), Université de Toulon (UTLN), Toxicologie Alimentaire (UTA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Nutrition humaine et lipides : Biodisponibilité, métabolisme et régulation, Université de la Méditerranée - Aix-Marseille 2-Institut National de la Recherche Agronomique (INRA)-Université de Provence - Aix-Marseille 1-IFR125-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de résonance magnétique biologique et médicale (CRMBM), Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Centre National de la Recherche Scientifique (CNRS), ToxAlim (ToxAlim), Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Les Thes de la Pagode Company, French Ministry of Ecology (as part of the ACTIVISME project), 'Conseil General des Alpes Maritimes' (as part of the HCSTOX project), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Les Thés de la Pagode Company, by the French Ministry of Ecology (as part of the ACTIVISME project) and by the 'Conseil Général des Alpes Maritimes' (as part of the HCSTOX project), Assistance Publique - Hôpitaux de Marseille (APHM)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), PROcessus de Transfert et d'Echanges dans l'Environnement - EA 3819 ( PROTEE ), Université de Toulon ( UTLN ), Toxicologie Alimentaire ( UTA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Université de la Méditerranée - Aix-Marseille 2-Institut National de la Recherche Agronomique ( INRA ) -Université de Provence - Aix-Marseille 1-IFR125-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Centre de résonance magnétique biologique et médicale ( CRMBM ), Aix Marseille Université ( AMU ) -Assistance Publique - Hôpitaux de Marseille ( APHM ) -Centre National de la Recherche Scientifique ( CNRS ), ToxAlim ( ToxAlim ), Institut National Polytechnique [Toulouse] ( INP ) -Institut National de la Recherche Agronomique ( INRA ) -Université Paul Sabatier - Toulouse 3 ( UPS ) -Ecole Nationale Vétérinaire de Toulouse, Braud, Laura, Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN), Université de Toulouse (UT)-Université de Toulouse (UT), and BERNARD, Monique
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Male ,Antioxidant ,tea ,antioxidant ,Time Factors ,Camellia sinensis ,polyphenols ,bioaccessibility ,EGCG ,hepatocytes ,ROS ,mitochondrial membrane integrity ,medicine.medical_treatment ,Pharmaceutical Science ,medicine.disease_cause ,Antioxidants ,Catechin ,Analytical Chemistry ,chemistry.chemical_compound ,membrane mitochondriale ,tert-Butylhydroperoxide ,Superoxides ,Drug Discovery ,Gallic acid ,Food science ,[ SDV.IB ] Life Sciences [q-bio]/Bioengineering ,bioaccessibilité ,ComputingMilieux_MISCELLANEOUS ,Chromatography, High Pressure Liquid ,chemistry.chemical_classification ,food and beverages ,Mitochondria ,Biochemistry ,Liver ,Chemistry (miscellaneous) ,Molecular Medicine ,[SDV.IB]Life Sciences [q-bio]/Bioengineering ,Caffeine ,Spectrometry, Mass, Electrospray Ionization ,Cell Survival ,Protective Agents ,Models, Biological ,Article ,lcsh:QD241-441 ,polyphénol ,Alkaloids ,lcsh:Organic chemistry ,Phenols ,medicine ,Animals ,Physical and Theoretical Chemistry ,[SDV.IB] Life Sciences [q-bio]/Bioengineering ,Reactive oxygen species ,Plant Extracts ,Organic Chemistry ,the ,Rats ,Oxidative Stress ,chemistry ,Hepatoprotection ,Polyphenol ,Hepatocytes ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,Oxidative stress - Abstract
International audience; Tea is an interesting source of antioxidants capable of counteracting the oxidative stress implicated in liver diseases. We investigated the impact of antioxidant molecules provided by a mixture of teas' leaves (green, oolong, pu-erh) after different infusion durations in the prevention of oxidative stress in isolated rat hepatocytes, by comparison with pure epigallocatechin-3-gallate (EGCG), the main representative of tea catechins. Dried aqueous tea extracts (ATE) obtained after 5, 15 and 30 min infusion time were characterized for total polyphenols (gallic acid equivalent), catechins, gallic acid and caffeine (HPLC-DAD/ESI-MS) contents, and for scavenging ability against 2,2-diphenyl-1-picrylhydrazyl free radical. Hepatoprotection was evaluated through hepatocyte viability tests using tert-butyl hydroperoxide as a stress inducer, (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, neutral red uptake, real-time cellular impedance) and mitochondrial function tests. We showed that a 5-min incubation time is sufficient for an optimal bioaccessibility of tea compounds with the highest antioxidative ability, which decreases for longer durations. A 4-h pretreatment of cells with ATE significantly prevented cell death by regulating reactive oxygen species production and maintaining mitochondrial integrity. Pure EGCG, at doses similar in ATE (5-12 µM), was inefficient, suggesting a plausible synergy of several water-soluble tea compounds to explain the ATE beneficial effects.
- Published
- 2015
26. Heterozygous RPA2 variant as a novel genetic cause of telomere biology disorders.
- Author
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Kochman R, Ba I, Yates M, Pirabakaran V, Gourmelon F, Churikov D, Laffaille M, Kermasson L, Hamelin C, Marois I, Jourquin F, Braud L, Bechara M, Lainey E, Nunes H, Breton P, Penhouet M, David P, Géli V, Lachaud C, Maréchal A, Revy P, Kannengiesser C, Saintomé C, and Coulon S
- Subjects
- Humans, Heterozygote, Male, Female, Shelterin Complex, Telomere Shortening genetics, Mutation, Telomerase genetics, Telomerase metabolism, Ubiquitination genetics, Ubiquitin-Protein Ligases genetics, Replication Protein A genetics, Replication Protein A metabolism, Telomere genetics, Telomere-Binding Proteins genetics, Telomere-Binding Proteins metabolism
- Abstract
Premature telomere shortening or telomere instability is associated with a group of rare and heterogeneous diseases collectively known as telomere biology disorders (TBDs). Here we identified two unrelated individuals with clinical manifestations of TBDs and short telomeres associated with the identical monoallelic variant c.767A>G; Y256C in RPA2 Although the replication protein A2 (RPA2) mutant did not affect ssDNA binding and G-quadruplex-unfolding properties of RPA, the mutation reduced the affinity of RPA2 with the ubiquitin ligase RFWD3 and reduced RPA ubiquitination. Using engineered knock-in cell lines, we found an accumulation of RPA at telomeres that did not trigger ATR activation but caused short and dysfunctional telomeres. Finally, both patients acquired, in a subset of blood cells, somatic genetic rescue events in either POT1 genes or TERT promoters known to counteract the accelerated telomere shortening. Collectively, our study indicates that variants in RPA2 represent a novel genetic cause of TBDs. Our results further support the fundamental role of the RPA complex in regulating telomere length and stability in humans., (© 2024 Kochman et al.; Published by Cold Spring Harbor Laboratory Press.)
- Published
- 2024
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27. Increased Sirt1 secreted from visceral white adipose tissue is associated with improved glucose tolerance in obese Nrf2-deficient mice.
- Author
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Braud L, Pini M, Stec DF, Manin S, Derumeaux G, Stec DE, Foresti R, and Motterlini R
- Subjects
- Adipose Tissue, White, Animals, Diet, High-Fat adverse effects, Glucose, Intra-Abdominal Fat, Mice, Mice, Inbred C57BL, Mice, Obese, NF-E2-Related Factor 2 genetics, Obesity genetics, Insulin Resistance genetics, Sirtuin 1 genetics
- Abstract
Obesity is associated with metabolic dysregulation characterized by insulin resistance and glucose intolerance. Nuclear factor E2-related factor (Nrf2) is a critical regulator of the stress response and Nrf2-deficient mice (Nrf2
-/- ) are protected against high fat diet (HFD)-induced metabolic derangement. We searched for factors that could underline this favorable phenotype and found that Nrf2-/- mice exhibit higher circulating levels of sirtuin 1 (Sirt1), a key player in cellular homeostasis and energy metabolism, compared to wild-type mice. Increased Sirt1 levels in Nrf2-/- mice were found not only in animals under standard diet but also following HFD. Interestingly, we report here that the visceral adipose tissue (eWAT) is the sole source of increased Sirt1 protein in plasma. eWAT and other fat depots displayed enhanced adipocytes lipolysis, increased fatty acid oxidation and glycolysis, suggesting autocrine and endocrine actions of Sirt1 in this model. We further demonstrate that removal of eWAT completely abolishes the increase in circulating Sirt1 and that this procedure suppresses the beneficial effect of Nrf2 deficiency on glucose tolerance, but not insulin sensitivity, following a HFD regime. Thus, in contrast to many other stressful conditions where Nrf2 deficiency exacerbates damage, our study indicates that up-regulation of Sirt1 levels specifically in the visceral adipose tissue of Nrf2-/- mice is a key adaptive mechanism that mitigates glucose intolerance induced by nutritional stress., (Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.)- Published
- 2021
- Full Text
- View/download PDF
28. Vascular endothelial function masks increased sympathetic vasopressor activity in rats with metabolic syndrome.
- Author
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Battault S, Meziat C, Nascimento A, Braud L, Gayrard S, Legros C, De Nardi F, Drai J, Cazorla O, Thireau J, Meyer G, and Reboul C
- Subjects
- Animals, Aorta metabolism, Diet, High-Fat, Dietary Sucrose, Disease Models, Animal, Endothelium, Vascular metabolism, Epinephrine blood, Heart Rate, Male, Metabolic Syndrome etiology, Metabolic Syndrome metabolism, Nitric Oxide metabolism, Nitric Oxide Synthase Type III metabolism, Norepinephrine blood, Rats, Wistar, Receptors, Adrenergic, alpha-1 metabolism, Signal Transduction, Sympathetic Nervous System metabolism, Aorta innervation, Arterial Pressure, Endothelium, Vascular innervation, Metabolic Syndrome physiopathology, Sympathetic Nervous System physiopathology, Vasoconstriction
- Abstract
Sympathetic hyperactivation, a common feature of obesity and metabolic syndrome, is a key trigger of hypertension. However, some obese subjects with autonomic imbalance present a dissociation between sympathetic activity-mediated vasoconstriction and increased blood pressure. Here, we aimed to determine in a rat model of metabolic syndrome whether the endothelium endothelial nitric oxide (NO) synthase (eNOS)-NO pathway contributes to counteract the vasopressor effect of the sympathetic system. Rats were fed a high-fat and high-sucrose (HFS) diet for 15 wk. Sympathovagal balance was evaluated by spectral analysis of heart rate variability and plasmatic catecholamine measurements. Blood pressure was measured in the presence or absence of N-nitro-l-arginine methyl ester (l-NAME) to inhibit the contribution of eNOS. Vascular reactivity was assessed on isolated aortic rings in response to α
1 -adrenergic agonist. The HFS diet increased sympathetic tone, which is characterized by a higher low on the high-frequency spectral power ratio and a higher plasmatic concentration of epinephrine. Despite this, no change in blood pressure was observed. Interestingly, HFS rats exhibited vascular hyporeactivity (-23.6%) to α1 -adrenergic receptor stimulation that was abolished by endothelial removal or eNOS inhibition (l-NAME). In addition, eNOS phosphorylation (Ser1177 ) was increased in response to phenylephrine in HFS rats only. Accordingly, eNOS inhibition in vivo revealed higher blood pressure in HFS rats compared with control rats (147 vs. 126 mmHg for mean blood pressure, respectively). Restrain of adrenergic vasopressor action by endothelium eNOS is increased in HFS rats and contributes to maintained blood pressure in the physiological range. NEW & NOTEWORTHY Despite the fact that prohypertensive sympathetic nervous system activity is markedly increased in rats with early metabolic syndrome, they present with normal blood pressure. These observations appear to be explained by increased endothelial nitric oxide synthase response to adrenergic stimulation, which results in vascular hyporeactivity to α-adrenergic stimulation, and therefore blood pressure is preserved in the physiological range. Listen to this article's corresponding podcast at http://www.physiology.org/doi/10.1152/ajpheart.00217.2017 .- Published
- 2018
- Full Text
- View/download PDF
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