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Effect of Brewing Duration on the Antioxidant and Hepatoprotective Abilities of Tea Phenolic and Alkaloid Compounds in a t-BHP Oxidative Stress-Induced Rat Hepatocyte Model
- Source :
- Molecules, Molecules, MDPI, 2015, 20 (8), pp.14985--15002. ⟨10.3390/molecules200814985⟩, Molecules, MDPI, 2015, 20 (8), pp.14985-15002. ⟨10.3390/molecules200814985⟩, Molecules, Vol 20, Iss 8, Pp 14985-15002 (2015), Molecules, MDPI, 2015, 20 (8), pp.14985--15002. 〈10.3390/molecules200814985〉, Molecules 8 (20), 14985-15002. (2015), Molecules, 2015, 20 (8), pp.14985--15002. ⟨10.3390/molecules200814985⟩, Volume 20, Issue 8, Pages 14985-15002
- Publication Year :
- 2015
- Publisher :
- MDPI, 2015.
-
Abstract
- International audience; Tea is an interesting source of antioxidants capable of counteracting the oxidative stress implicated in liver diseases. We investigated the impact of antioxidant molecules provided by a mixture of teas' leaves (green, oolong, pu-erh) after different infusion durations in the prevention of oxidative stress in isolated rat hepatocytes, by comparison with pure epigallocatechin-3-gallate (EGCG), the main representative of tea catechins. Dried aqueous tea extracts (ATE) obtained after 5, 15 and 30 min infusion time were characterized for total polyphenols (gallic acid equivalent), catechins, gallic acid and caffeine (HPLC-DAD/ESI-MS) contents, and for scavenging ability against 2,2-diphenyl-1-picrylhydrazyl free radical. Hepatoprotection was evaluated through hepatocyte viability tests using tert-butyl hydroperoxide as a stress inducer, (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, neutral red uptake, real-time cellular impedance) and mitochondrial function tests. We showed that a 5-min incubation time is sufficient for an optimal bioaccessibility of tea compounds with the highest antioxidative ability, which decreases for longer durations. A 4-h pretreatment of cells with ATE significantly prevented cell death by regulating reactive oxygen species production and maintaining mitochondrial integrity. Pure EGCG, at doses similar in ATE (5-12 µM), was inefficient, suggesting a plausible synergy of several water-soluble tea compounds to explain the ATE beneficial effects.
- Subjects :
- Male
Antioxidant
tea
antioxidant
Time Factors
Camellia sinensis
polyphenols
bioaccessibility
EGCG
hepatocytes
ROS
mitochondrial membrane integrity
medicine.medical_treatment
Pharmaceutical Science
medicine.disease_cause
Antioxidants
Catechin
Analytical Chemistry
chemistry.chemical_compound
membrane mitochondriale
tert-Butylhydroperoxide
Superoxides
Drug Discovery
Gallic acid
Food science
[ SDV.IB ] Life Sciences [q-bio]/Bioengineering
bioaccessibilité
ComputingMilieux_MISCELLANEOUS
Chromatography, High Pressure Liquid
chemistry.chemical_classification
food and beverages
Mitochondria
Biochemistry
Liver
Chemistry (miscellaneous)
Molecular Medicine
[SDV.IB]Life Sciences [q-bio]/Bioengineering
Caffeine
Spectrometry, Mass, Electrospray Ionization
Cell Survival
Protective Agents
Models, Biological
Article
lcsh:QD241-441
polyphénol
Alkaloids
lcsh:Organic chemistry
Phenols
medicine
Animals
Physical and Theoretical Chemistry
[SDV.IB] Life Sciences [q-bio]/Bioengineering
Reactive oxygen species
Plant Extracts
Organic Chemistry
the
Rats
Oxidative Stress
chemistry
Hepatoprotection
Polyphenol
Hepatocytes
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Oxidative stress
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 20
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....a66534d2ed83e693edd97a0ee7ed83c6