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Your search keyword '"Bramlage, Birgit"' showing total 14 results
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14 results on '"Bramlage, Birgit"'

1. Targeted siRNA Delivery and mRNA Knockdown Mediated by Bispecific Digoxigenin-binding Antibodies

Author

Schneider, Britta, Grote, Michael, John, Matthias, Haas, Alexander, Bramlage, Birgit, lckenstein, Ludger M, Jahn-Hofmann, Kerstin, Bauss, Frieder, Cheng, Weijun, Croasdale, Rebecca, Daub, Karin, Dill, Simone, Hoffmann, Eike, Lau, Wilma, Burtscher, Helmut, Ludtke, James L, Metz, Silke, Mundigl, Olaf, Neal, Zane C, Scheuer, Werner, Stracke, Jan, Herweijer, Hans, and Brinkmann, Ulrjch

Published
2012
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2. Therapeutic RNAi Targeting PCSK9 Acutely Lowers Plasma Cholesterol in Rodents and LDL Cholesterol in Nonhuman Primates

Author

Frank-Kamenetsky, Maria, Grefhorst, Aldo, Anderson, Norma N., Racie, Timothy S., Bramlage, Birgit, Akinc, Akin, Butler, David, Charisse, Klaus, Dorkin, Robert, Fan, Yupeng, Gamba-Vitalo, Christina, Hadwiger, Philipp, Jayaraman, Muthusamy, John, Matthias, Jayaprakash, K. Narayanannair, Maier, Martin, Nechev, Lubomir, Rajeev, Kallanthottathil G., Read, Timothy, Röhl, Ingo, Soutschek, Jürgen, Tan, Pamela, Wong, Jamie, Wang, Gang, Zimmermann, Tracy, de Fougerolles, Antonin, Vornlocher, Hans-Peter, Langer, Robert, Anderson, Daniel G., Manoharan, Muthiah, Koteliansky, Victor, Horton, Jay D., and Fitzgerald, Kevin

Published
2008
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3. Therapeutic silencing of an endogenous gene by systemic administration of modified siRNAs

Author

Soutschek, Jurgen, Akinc, Akin, Bramlage, Birgit, Charisse, Klaus, Constien, Rainer, Donoghue, Mary, Elbashir, Sayda, Geick, Anke, Hadwiger, Philipp, Harborth, Jens, John, Matthias, Kesavan, Venkitasamy, Lavine, Gary, Pandey, Rajendra K., Racie, Timothy, Rajeev, Kallanthottathil G., Rohl, Ingo, Toudjarska, Ivanka, Wang, Gang, Wuschko, Silvio, Bumcrot, David, Koteliansky, Victor, Limmer, Stefan, Manoharan, Muthiah, and Vornlocher, Hans-Peter

Subjects
Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Author(s): Jürgen Soutschek (corresponding author) [1]; Akin Akinc [2]; Birgit Bramlage [1]; Klaus Charisse [2]; Rainer Constien [1]; Mary Donoghue [2]; Sayda Elbashir [2]; Anke Geick [1]; Philipp Hadwiger [1]; [...]

Published
2004
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4. RNAi-mediated gene silencing in non-human primates

Author

Zimmermann, Tracy S., Lee, Amy C. H., Akinc, Akin, Bramlage, Birgit, Bumcrot, David, Fedoruk, Matthew N., Harborth, Jens, Heyes, James A., Jeffs, Lloyd B., John, Matthias, Judge, Adam D., Lam, Kieu, McClintock, Kevin, Nechev, Lubomir V., Palmer, Lorne R., Racie, Timothy, Röhl, Ingo, Seiffert, Stephan, Shanmugam, Sumi, Sood, Vandana, Soutschek, Jürgen, Toudjarska, Ivanka, Wheat, Amanda J., Yaworski, Ed, Zedalis, William, Koteliansky, Victor, Manoharan, Muthiah, Vornlocher, Hans-Peter, and MacLachlan, Ian

Published
2006

5. Characterization of a monoclonal antibody with specificity for holo-transcobalamin

Author

Fedosov Sergey N, Brady Jeff, Campbell Andrew, Rian Anne, Orning Lars, Bramlage Birgit, Thompson Keith, and Quadros Edward V

Subjects
Nutrition. Foods and food supply, TX341-641, Nutritional diseases. Deficiency diseases, RC620-627
Abstract

Abstract Background Holotranscobalamin, cobalamin-saturated transcobalamin, is the minor fraction of circulating cobalamin (vitamin B12), which is available for cellular uptake and hence is physiologically relevant. Currently, no method allows simple, direct quantification of holotranscobalamin. We now report on the identification and characterization of a monoclonal antibody with a unique specificity for holotranscobalamin. Methods The specificity and affinity of the monoclonal antibodies were determined using surface plasmon resonance and recombinant transcobalamin as well as by immobilizing the antibodies on magnetic microspheres and using native transcobalamin in serum. The epitope of the holotranscobalamin specific antibody was identified using phage display and comparison to a de novo generated three-dimensional model of transcobalamin using the program Rosetta. A direct assay for holotrnscobalamin in the ELISA format was developed using the specific antibody and compared to the commercial assay HoloTC RIA. Results An antibody exhibiting >100-fold specificity for holotranscobalamin over apotranscobalamin was identified. The affinity but not the specificity varied inversely with ionic strength and pH, indicating importance of electrostatic interactions. The epitope was discontinuous and epitope mapping of the antibody by phage display identified two similar motifs with no direct sequence similarity to transcobalamin. A comparison of the motifs with a de novo generated three-dimensional model of transcobalamin identified two structures in the N-terminal part of transcobalamin that resembled the motif. Using this antibody an ELISA based prototype assay was developed and compared to the only available commercial assay for measuring holotranscobalamin, HoloTC RIA. Conclusion The identified antibody possesses a unique specificity for holotranscobalamin and can be used to develop a direct assay for the quantification of holotranscobalamin.

Published
2006
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6. Abstract 583: RNAi Therapeutics For The Lowering Of Cholesterol

Author

Fitzgerald, Kevin, primary, Frank-Kamenetsky, Maria, additional, Zimmermann, Tracy S, additional, Horton, Jay, additional, Akinc, Akin, additional, Bramlage, Birgit, additional, Bumcrot, David, additional, Harborth, Jens, additional, John, Matthias, additional, Kotelianski, Victor, additional, Manoharan, Muthiah, additional, Maier, Martin, additional, Nechev, Lubomir, additional, Racie, Timothy, additional, Roehl, Ingo, additional, Seiffert, Stephan, additional, Shanmugam, Sumi, additional, Soutschek, Jurgen, additional, Toudjarska, Ivanka, additional, Vornlocher, Hans-Peter, additional, and Zedalis, William, additional

Published
2007
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