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Halophilic Halomonas bluephagenesis , a natural producer of poly-3-hydroxybutyrate (PHB), was metabolically engineered to synthesize ethylene glycol and glycolate from xylose. Xylose utilization was achieved by overexpressing either the xylonate pathway or the ribulose-1-phosphate pathway. The key genes encoding for xylonate dehydratase and 2-keto-3-deoxy-xylonate aldolase in the xylonate pathway were screened. With further overexpressing aldehyde reductase gene yjgB , ethylene glycol accumulation was improved to 0.91 g/L, accompanied with 1.48 g/L of PHB accumulation. The disruption of native glycolate oxidase was found to be essential for glycolate production, and the defective recombinant strain produced 0.80 g/L glycolate with 1.14 g/L PHB in shake flask cultures. These results indicated that H. bluephagenesis has the potential to produce diverse metabolic chemicals from xylose. • H. bluephagenesis was firstly engineered to produce ethylene glycol and glycolate. • Two xylose assimilation pathways were established in H. bluephagenesis. • Ethylene glycol and glycolate titers reached 0.91 g/L and 0.80 g/L in shake flasks, respectively. [ABSTRACT FROM AUTHOR]

There is increasing interest in the discovery and application of molecular flasks–supramolecular host structures capable of catalyzing organic reactions. Reminiscent of enzymes due to possessing a host cavity akin to an active site, molecular flasks can exhibit complex catalytic mechanisms and in many cases provide selectivity not achievable in bulk solvent. In this Review, we aim to organize the increasingly diverse examples through a two‐part structure. In part one, we provide an overview of the different acceleration modes that operate within molecular flasks, while in part two we showcase, through selected examples, the different types of selectivity that are obtainable through the use of molecular flasks. Particular attention is given to examples that are relevant to current challenges in synthetic organic chemistry. We believe that this structure makes the field more approachable and thus will stimulate the development of novel applications of molecular flasks. [ABSTRACT FROM AUTHOR]

A new kind of open-end optical bottle formed by launching the circular Airy Gaussian vortex beams (CAGVBs) in the parabolic potential is demonstrated. Compared to the circular Airy beams, the CAGVBs in the parabolic potential still maintain abruptly auto-focusing property, but their focus spot is hollow. The focal intensity and the focal position can be adjusted by choosing different scaling factor, topological charge and potential depth. What's more, a closed-end or open-end optical bottle can be obtained by imposing two off-axis optical vortices rotating in the opposite or the same direction on the circular Airy Gaussian beams, respectively. Besides, when imposing one vortex in the radially and azimuthally polarized circular Airy Gaussian beams (RPCAGBs and APCAGBs), the topological charge of the vortices of the RPCAGBs and APCAGBs will affect the open state of RPCAGBs and APCAGBs. [ABSTRACT FROM AUTHOR]

Highlights Acoustic emission (AE) serves as a non‐destructive testing (NDT) technique with unique potential for assessing structural integrity and potentially predicting failures in pressure bottles. The authors have effectively demonstrated its applicability in predicting burst pressures of glass fiber reinforced plastic (GFRP) pressure bottles. In their study, five identical GFRP pressure bottles underwent pressurization cycles, and the AE data collected during these cycles was analyzed to develop a straightforward empirical relationship for predicting burst performance. This approach highlights a significant finding: impending failure can be detected with notable accuracy even when pressure levels are at 50%–60% of the maximum expected operating pressure (MEOP), with a reasonable margin of error. Notably, there exists a gap in the existing literature concerning clear methodologies for predicting burst pressures of composite pressure bottles. This methodology, however, can be extended beyond GFRP to predict the burst pressure of pressure bottles made from other material systems in real‐time. The composite pressure bottle failure is predicted at very low pressure. The prediction is at a range of 50%–75% of MEOP. To predict the burst pressure of GFRP pressure bottle an empirical formula is generated. The empirical formula is generated using Acoustic Emission parameters. Major AE parameters are determined from a set of GFRP pressure bottles. [ABSTRACT FROM AUTHOR]

Food packaging plays a crucial role in product storage, transportation and distribution. This study is dedicated to the development of high barrier blends for food packaging through the melt blending of polyethylene terephthalate (PET) and polyethylene 2,6‐naphthalate (PEN). The blends underwent comprehensive characterization involving chromaticity analysis, DSC, FTIR and XRD. Results indicated that the blend comprising 30 wt% PEN and 70 wt% PET exhibited superior structural properties, making it suitable for the production of beverage packing bottles. To evaluate the bottle properties, SEM, AFM, TG analysis and permeability testing were conducted. Comparatively, the barriers to O2, CO2, and water vapour of PEN/PET bottles were 3.52‐fold, 7.36‐fold and 2.25‐fold higher than PET bottles, and the mechanical properties and UV blocking properties have also improved. The PEN/PET bottles exhibited good thermal stability, with thermal shrinkage temperature and initial decomposition temperature at 87.5°C and 254°C, significantly higher than PET bottles (p < 0.05). Interestingly, the thermal shrinkage rate of PEN/PET bottles was measured at 87.5%, notably lower than that of PET bottles at 90.9%, indicating that PEN/PET bottles had good thermal stability. Additionally, beverage stored in PEN/PET bottles demonstrated lower dissolved oxygen content, suggesting promising applications for PEN/PET bottles in beverage packaging. [ABSTRACT FROM AUTHOR]

For each surface besides the sphere, projective plane, and Klein bottle, we construct a face‐simple minimal quadrangulation, that is, a simple quadrangulation on the fewest number of vertices possible, whose dual is also a simple graph. Our result answers a question of Liu, Ellingham, and Ye while providing a simpler proof of their main result. The inductive construction is based on an earlier idea for finding near‐quadrangular embeddings of the complete graphs using the diamond sum operation. [ABSTRACT FROM AUTHOR]

Microbial biotransformation is valuable for obtaining pharmaceutical, nutritional, and cosmetical potential molecules. Basically, for its realization, only a parental molecule, a microorganism, and a suitable fermentation system are needed. Regarding the last requirement, the most common resource used for screenings are the shaken flasks (a.k.a., conical flasks or Erlenmeyers). Despite their popularity, several concerning points discussed here limit their utilization. Multiparametric bioreactors give more reliable results but are expensive and the need for many of them for parallelized screenings becomes inaccessible for many research groups. In this review, we present some problems associated with this issue and strategies to solve them. [ABSTRACT FROM AUTHOR]

The biosensors based on transcription factors (TFs) are widely used in high throughput screening of metabolic overproducers. The unsatisfactory performances (narrow detection and dynamic ranges) of biosensors limit their practical application and need more improvement. In this study, using the TF LysG (sensing lysine) as an example, a biosensor optimization method was constructed by growth-coupled screening of TF random mutant libraries. The better the performance of the biosensor, the faster the strain grows under screening pressure. A LysGE15D, A54D, and I164V-based biosensors were obtained, which were about 2-fold of the control in the detection and dynamic ranges. A lysine high-producer was screened effectively using the optimized biosensor with the production at 1.51 ± 0.30 g/L in flasks (2.22-fold of the original strain). This study provided a promising strategy for optimizing TF-based biosensors and was of high potential to be applied in the lysine high-producers screening process. [ABSTRACT FROM AUTHOR]

The current manufacturing disruption of BACTEC blood culture bottles has drawn attention to diagnostic stewardship around blood culture utilization. In this perspective, we offer strategies for implementing blood culture stewardship using a graded approach based on a hospital's blood culture bottle supply. These strategies should inform plans to mitigate the impact of the shortage on patient care and reinforce fundamental principles of blood culture stewardship. [ABSTRACT FROM AUTHOR]

An inverted container of water drains with a periodic glugging process where bubbles of air enter through the bottom hole, thus replacing the exiting water. If the container is soft, its walls can flex with each glug. We examine the effect of container elasticity on the drainage process. Experiments are conducted to measure the drainage rate and the glug period as water drains out of a container through a bottom hole. The container compliance is varied by varying the diameter of a soft rubber membrane comprising the lid of the container. Increasing container compliance (i.e., making the container softer) is found to increase the drainage velocity, increase the glugging period, and increase the volume of each glug. Previously, Clanet and Searby (2004) modeled the glugging process as a spring-and-mass oscillator where the compressibility of air in the headspace acts as the spring. We adapt this model so that the membrane elasticity acts in series with the air compressibility. This adapted model qualitatively agrees with the observations but underpredicts the observed effect on the glug period. [ABSTRACT FROM AUTHOR]

We prove that every triangulation of either of the torus, projective plane and Klein bottle, contains a vertex-spanning planar Laman graph as a subcomplex. Invoking a result of Király, we conclude that every 1-skeleton of a triangulation of a surface of nonnegative Euler characteristic has a rigid realization in the plane using at most 26 locations for the vertices. [ABSTRACT FROM AUTHOR]

The article focuses on Richard Hague's reflection on a transformative experience from his youth at the Abbey of Gethsemane in Kentucky, which left a lasting spiritual impression on him. Topics include his admiration for the monks' simple, disciplined life, the sensory connection to nature he experienced there, and how that moment in his life continued to resonate decades later, even as he stood in Iceland.

Copyright of Arts for Linguistic & Literary Studies is the property of Thamar University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This article shows an analysis of selected stages of a machine's life cycle environmental impact in the specific case of machines that package bottles in thermo-shrinkable film. As part of this analysis, laboratory tests were carried out to compare the performance properties of polyethylene films (with and without recycled material). Then, a life cycle assessment (LCA) was carried out within the specified system boundaries using the SimaPro program. Using the ReCiPe 2016 method, differences in the impact of the mass bottle packaging process on the categories human health, ecosystems and resources were determined depending on the shrink film used in the process. These tests showed that the tested batch of film with the addition of recyclates has similar functional properties to traditional ones and can therefore be used in the mass packaging process. The environmental analysis showed that changing the type of film to film with the addition of recyclates results in an almost 70% reduction in the potential negative impact of the process in terms of damage to health and ecosystems, and by 85% in terms of resources. [ABSTRACT FROM AUTHOR]

We present a new boundary condition for simulations of magnetic reconnection based on the topology of a Klein bottle. When applicable, the new condition is computationally cheaper than fully periodic boundary conditions, reconnects more flux than systems with conducting boundaries, and does not require assumptions about regions external to the simulation as is necessary for open boundaries. The new condition reproduces the expected features of reconnection but cannot be straightforwardly applied in systems with asymmetric upstream plasmas. [ABSTRACT FROM AUTHOR]

Copyright of Packaging & Food Machinery is the property of Packaging & Food Machinery Magazine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Purpose: Simultaneous membrane-based feeding and monitoring of the oxygen transfer rate shall be introduced to the newly established perforated ring flask, which consists of a cylindrical glass flask with an additional perforated inner glass ring, for rapid bioprocess development. Methods: A 3D-printed adapter was constructed to enable monitoring of the oxygen transfer rate in the perforated ring flasks. Escherichia coli experiments in batch were performed to validate the adapter. Fed-batch experiments with different diffusion rates and feed solutions were performed. Results: The adapter and the performed experiments allowed a direct comparison of the perforated ring flasks with Erlenmeyer flasks. In batch cultivations, maximum oxygen transfer capacities of 80 mmol L−1 h−1 were reached with perforated ring flasks, corresponding to a 3.5 times higher capacity than in Erlenmeyer flasks. Fed-batch experiments with a feed reservoir concentration of 500 g glucose L−1 were successfully conducted. Based on the oxygen transfer rate, an ammonium limitation could be observed. By adding 40 g ammonium sulfate L−1 to the feed reservoir, the limitation could be prevented. Conclusion: The membrane-based feeding, an online monitoring technique, and the perforated ring flask were successfully combined and offer a new and promising tool for screening and process development in biotechnology. [ABSTRACT FROM AUTHOR]

Background: The Centers for Disease Control and Prevention (CDC) bottle bioassay is a commonly used susceptibility test for measuring insect response to insecticide exposure. However, inconsistencies and high variability in insect response when conducting CDC bottle bioassays have been reported in previous publications. We hypothesized that the CDC bottle bioassay results may be compromised when expected and actual insecticide concentrations in the bottles are not equivalent and that inadequate bottle cleaning and/or loss during insecticide introduction and bottle storage steps could be responsible. We explored this hypothesis by quantifying insecticides using gas chromatography tandem mass spectrometry (GC-MS/MS) in bottles that had been cleaned, prepared, and stored according to the CDC guidelines. Methods: We investigated the bottle cleaning, preparation, and storage methods outlined in the CDC bottle bioassay procedure to identify sources of irreproducibility. We also investigated the effectiveness of cleaning bottles by autoclaving because this method is commonly used in insecticide assessment laboratories. The two insecticides used in this study were chlorpyrifos and lambda-cyhalothrin (λ-cyhalothrin). Insecticides were removed from glass bioassay bottles by rinsing with ethyl-acetate and n-hexane and then quantified using GC-MS/MS. Results: The CDC bottle bioassay cleaning methods did not sufficiently remove both insecticides from the glass bottles. The cleaning methods removed chlorpyrifos, which has higher water solubility, more effectively than λ-cyhalothrin. Chlorpyrifos experienced significant loss during the bottle-coating process whereas λ-cyhalothrin did not. As for bottle storage, no significant decreases in insecticide concentrations were observed for 6 h following the initial drying period for either insecticide. Conclusions: The CDC bottle bioassay protocol is susceptible to producing inaccurate results since its recommended bottle cleaning method is not sufficient and semi-volatile insecticides can volatilize from the bottle during the coating process. This can lead to the CDC bottle bioassay producing erroneous LC50 values. High levels of random variation were also observed in our experiments, as others have previously reported. We have outlined several steps that CDC bottle bioassay users could consider that would lead to improved accuracy and reproducibility when acquiring toxicity data. [ABSTRACT FROM AUTHOR]

In this paper, we study the upper bounds for the topological complexity of the total spaces of some classes of fibre bundles. We calculate a tight upper bound for the topological complexity of an n-dimensional Klein bottle. We also compute the exact value of the topological complexity of a 3-dimensional Klein bottle. We describe the cohomology rings of several classes of generalized projective product spaces with ℤ2-coefficients. Then we study the LS-category and topological complexity of infinite families of generalized projective product spaces. We reckon the exact value of these invariants in many specific cases. We calculate the equivariant LS-category and equivariant topological complexity of several product spaces equipped with ℤ2-action. [ABSTRACT FROM AUTHOR]

Shake flask cultivation, a cornerstone in bioprocess research encounters limitations in supplying sufficient oxygen and exchanging gases, restricting its accuracy in assessing microbial growth and metabolic activity. In this communication, we introduce an innovative gas supply apparatus that harnesses the rotational motion of a shaking incubator to facilitate continuous air delivery, effectively overcoming these limitations. We measured the mass transfer coefficient (kLa) and conducted batch cultures of Corynebacterium glutamicum H36LsGAD using various working volumes to assess its performance. Results demonstrated that the gas supply apparatus significantly outperforms conventional silicone stoppers regarding oxygen delivery, with kLa values of 2531.7 h−1 compared to 20.25 h−1 at 230 rpm. Moreover, in batch cultures, the gas supply apparatus enabled substantial improvements in microbial growth, maintaining exponential growth even at larger working volumes. Compared to the existing system, an increase in final cell mass by a factor of 3.4‐fold was observed when utilizing 20% of the flask's volume, and a remarkable 9‐fold increase was achieved when using 60%. Furthermore, the gas supply apparatus ensured consistent oxygen supply and efficient gas exchange within the flask, overcoming challenges associated with low working volumes. This approach offers a simple yet effective solution to enhance gas transfer in shake flask cultivation, bridging the gap between laboratory‐scale experiments and industrial fermenters. Its broad applicability holds promise for advancing research in bioprocess optimization and scale‐up endeavors. [ABSTRACT FROM AUTHOR]

Bottles and teats are ubiquitously used for feeding infants and young children. Yet there are limited empirical studies on the scope of infant feeding bottles, their attributes, or their marketing claims. We report the first comprehensive survey on infant feeding bottles and teats in Germany. We aimed to explore the extent of bottles and teats available in Germany, describe their physical attributes and analyze their marketing claims. A cross‐sectional survey of German bottle and teat manufacturer websites was conducted between June and November 2022. Product attributes are presented with descriptive statistics and photographs. Marketing claims are summarized in a descriptive content analysis. We identified 41 brands encompassing 447 unique products (226 bottles, 221 teats). The majority of bottles were plastic (147, 65%) or glass (64, 28%), and the majority of teats were silicone (188, 85%). Most brands (38, 93%) promoted products using one or more inappropriate marketing claims, including equivalency to breastfeeding (29, 73%), idealization through technical or medical descriptions (23, 58%), claims on disease prevention (31, 78%), references to naturalness (29, 73%), infant autonomy (10, 25%), and endorsements from parents (10, 25%) or health professionals (11, 28%). The majority of bottles and teats available in Germany appear to be marketed inappropriately and hold the potential to undermine public health recommendations on infant and young child feeding. Therefore, we recommend Germany strengthens legislation on the marketing of bottles and teats in accordance with the International Code of Marketing of Breastmilk Substitutes. Key messages: There is limited research on infant feeding bottles and teats or their marketing claims.Most infant feeding bottle brands identified (38, 93%) promoted products using one or more inappropriate marketing claims.No national legislation currently exists to restrict misleading marketing claims on infant feeding bottles and teats.Large bottle volumes, teats designed for feeding complementary foods and nonstandardized language for teat flow rates may promote overfeeding, overweight and obesity.Bottles and teats should be made of safe materials, be designed for easy cleaning, meet specified European standards and reference the European safety standard. [ABSTRACT FROM AUTHOR]

Purpose: To evaluate the performance of the rapid colorimetric polymyxin B microelution (RCPEm) in determining polymyxin B resistance directly from Enterobacterales-positive blood cultures. Methods: A set volume of positive blood culture bottles (diluted 1:10) was inoculated into a glucose-broth-phenol red solution (NP solution), where a polymyxin B disk was previously eluted (final concentration of 3 µg/mL). Test was read each 1 h for up to 4 h. Color change from red/orange to yellow indicated resistant isolates. Results were compared to the reference method, broth microdilution (BMD), performed from colonies grown on solid media from the same blood culture bottle. Results: One hundred fifty-two Enterobacterales-positive blood cultures were evaluated, 22.4% (34/152) of them resistant to polymyxin B (including 6.6% with borderline MICs). When performing directly from positive blood cultures (RCPEm-BC), specificity and sensitivity were 99.1% and 94.1%, respectively. Of note, 79.4% (27/34) of truly resistant isolates required 3 h of incubation, compared to the 18 ± 2 h incubation that microtiter plates of BMD demand before reading can be performed. Conclusions: RCPEm directly from blood cultures has great potential to be part of the routine of clinical microbiology laboratories to establish polymyxin B susceptibility, impacting outcome of patients with bloodstream infections caused by carbapenem-resistant Enterobacterales. [ABSTRACT FROM AUTHOR]

In the United States, a generic Type A medicated article product can gain the FDA approval by demonstrating bioequivalence (BE) to the pioneer product by successfully conducting a blood level, pharmacodynamic, or clinical BE study. A biowaiver can be granted based on several criteria, assuming the dissolution of the test and reference products represents the only factor influencing the relative bioavailability of both products. Monensin is practically insoluble in H2O per the USP definition. Previously published data from a comparison study of monensin dissolution profiles from the pioneer product and four generic products using biorelevant media showed that generic monensin products demonstrated different dissolution profiles to the pioneer product in these USP biorelevant rumen media. This follow‐up study compared the solubility profiles in simulated intestinal fluid (cFaSSIF, pH 7.5) for the pioneer product and four generic products. The generic monensin products demonstrated different in vitro dissolution profiles to the pioneer product in biorelevant media. The differences demonstrated in solubility and dissolution profiles are of concern regarding the potential efficacy of generic monensin in cattle. There are also additional concerns for the potential development of Eimeria resistance in cattle receiving a sub‐therapeutic dose of monensin from a less soluble generic product. [ABSTRACT FROM AUTHOR]

Findings on the effects of alcohol warning labels (AWLs) as a harm reduction tool have been mixed. This systematic review synthesized extant literature on the impact of AWLs on proxies of alcohol use. PsycINFO, Web of Science, PubMED, and MEDLINE databases and reference lists of eligible articles. Following PRISMA guidelines, 1,589 articles published prior to July 2020 were retrieved via database and 45 were via reference lists (961 following duplicate removal). Article titles and abstracts were screened, leaving the full text of 96 for review. The full-text review identified 77 articles meeting inclusion/exclusion criteria which are included here. Risk of bias among included studies was examined using the Evidence Project risk of bias tool. Findings fell into five categories of alcohol use proxies including knowledge/awareness, perceptions, attention, recall/recognition, attitudes/beliefs, and intentions/behavior. Real-world studies highlighted an increase in AWL awareness, alcohol-related risk perceptions (limited findings), and AWL recall/recognition post-AWL implementation; these findings have decreased over time. Conversely, findings from experimental studies were mixed. AWL content/formatting and participant sociodemographic factors also appear to influence the effectiveness of AWLs. Findings suggest conclusions differ based on the study methodology used, favoring real-world versus experimental studies. Future research should consider AWL content/formatting and participant sociodemographic factors as moderators. AWLs appear to be a promising approach for supporting more informed alcohol consumption and should be considered as one component in a comprehensive alcohol control strategy. [ABSTRACT FROM AUTHOR]

A novel approach for the quantification of recycled polyethylene terephthalate (r-PET) in commercial bottles is presented. Fifty-eight bottle samples from several brands and producers containing different percentages of r-PET were purchased from the market. Samples were analyzed by two spectroscopic methods: near-infrared (NIR) spectroscopy and attenuated total reflection (ATR) spectroscopy in the mid-infrared (MIR) region. No chemical pre-treatment was applied before analyses. The spectra were analyzed by partial-least squares (PLS) regression, and two models for NIR and MIR data were computed. Then, a multi-block regression was applied to join the two datasets. All models were validated by cross-validation and by excluding and projecting onto the model the replicated spectra of one sample at a time. Results demonstrated the potential of this approach, especially considering the variability of commercial samples in terms of additives, shape, or thickness of the bottles: for samples close to the centroids of the models (i.e., from 10 to 50% r-PET), the predictions of multi-block method seldom departed from the expected values of ±10%. Only for samples with 0% declared r-PET, the models showed poor prediction abilities. [ABSTRACT FROM AUTHOR]

In this regard, this work spent a year studying the process of photodestruction of painted recycled polyethylene terephthalate bottles in natural (Central Asia) and accelerated (UV climatic chamber) conditions under the influence of UV radiation. As a result of the studies, it was found that the average molecular weight of the transparent sample decreased to 7-8%, and the average molecular weight of the colored sample decreased to 15-36% in two different conditions. The resulting RPET color sources, in turn, worsen the physical and mechanical properties of the fiber after processing. [ABSTRACT FROM AUTHOR]

Bloodstream infections (BSIs) caused by multidrug-resistant bacteria are a critical life-threatening challenge which necessitates the urgency to trigger life-saving treatment in a timely manner. This study aimed to evaluate the time required for rapid detection of carbapenemase-producing Enterobacterales (CPE) directly from blood culture bottles to optimize empirical treatment of BSI, especially in pediatric and infant patients, using a cost-effective method. This study included 419 Gram-negative bacteria, of which Klebsiella pneumoniae and Escherichia coli were the most common CPE causing BSI in pediatric and neonatal patients. Phenotypic and genotypic resistance of the selected isolates (45 K. pneumoniae and 9 E. coli) were determined by VITEK-2 Compact system and PCR, respectively. BACT/ALERT bottles were spiked with isolates. Finally, colorimetric RESIST-BC assay and Vitek-2 compact system were evaluated for the rapid detection of carbapenem-resistant bacteria directly from positive blood culture bottles. All selected isolates were phenotypically resistant to carbapenems. PCR showed that bla NDM and bla OXA-48 were present in all isolates, bla VIM was present in 44.4%, while bla KPC and bla IMP were entirely absent. The RESIST-BC kit showed good agreement with PCR for bla NDM and bla OXA-48, demonstrating high sensitivity and specificity, but not with bla VIM. These findings point out that RESIST-BC assay demonstrated an exceptionally short detection time for CPE, completing all cases within the first hour after the blood culture bottles flagged positive. It is also superior in providing a clue for clinicians on antibiotic combinations that can be administered, depending on the type of β-lactamases detected, promptly and efficiently, with low expenses. [ABSTRACT FROM AUTHOR]

Non-Saccharomyces yeasts, particularly Metschnikowia pulcherrima, are considered alternatives to SO2 in winemaking, combating specific microorganisms. The sensory profile of the wine is contingent upon the type of yeast, the fermentation conditions, and the concentration and mode of application with Saccharomyces cerevisiae strains (whether pure or used in mixed/sequential co-fermentation). This study assessed the aroma in red wines produced with S. cerevisiae (Sc) and M. pulcherrima (Mp, non-Sc), incorporating variations in the method of addition and the inclusion or exclusion of SO2. The enological parameters of the wines were slightly affected. Volatile compounds were analysed in the wines through gas chromatography–mass spectrometry (GC-MS) at three moments: at the end of malolactic fermentation (MLF) and after 6 and 9 months of bottle ageing. Sequential fermentation of Sc and Mp reduced the concentration of most identified alcohols and acids, which is favourable, as these compounds can yield undesirable aromas at high concentrations. Regardless of the yeast mixture and Mp dose, a majority of the acetate esters and ethyl esters were quantified at concentrations above their perception thresholds, thus enhancing the sensory quality of the wines. Sensory analysis of wines showed generally positive evaluations. Using non-Saccharomyces as an alternative to SO2 improves the aromatic profile of wines. [ABSTRACT FROM AUTHOR]

During the machine vision inspection of the inner section of bottle caps within pharmaceutical packaging, the unique conca bottom and convex side walls often create obstructions to the illumination. Consequently, this results in challenges such as irregular background and diminished feature contrast in the image, ultimately leading to the misidentification of defects. As a solution, a vision system characterized by a Low-Angle and Large Divergence Angle (LALDA) is presented in this paper. Using the large divergence angle of LED, combined with low-angle illumination, a uniform image of the side wall region with bright-field characteristics and a uniform image of inner circle region at the bottom with dark-field characteristics are obtained, thus solving the problems of light being obscured and brightness overexposure of the background. Based on the imaging characteristics of LALDA, a multi-channel segmentation (MCS) algorithm is designed. The HSV color space has been transformed, and the image is automatically segmented into multiple sub-regions by mutual calculation of different channels. Further, image homogenization and enhancement are used to eliminate fluctuations in the background and to enhance the contrast of defects. In addition, a variety of defect extraction methods are designed based on the imaging characteristics of different sub-regions, which can avoid the problem of over-segmentation in detection. In this paper, the LALDA is applied to the defect detection inside the cap of capsule medicine bottle, the detection speed is better than 400 pcs/min and the detection accuracy is better than 95%, which can meet the actual production line capacity and detection requirements. [ABSTRACT FROM AUTHOR]

An innovative flavored wine was developed by macerating six different edible flowers into Chardonnay wine, where the physicochemical characteristics (titratable acidity, pH), antioxidant activity (DPPH, FRAP) and volatile profile were modulated. Bottle aging of the flower-flavored wines were performed for 9 months where a significant (p < 0.05) increases of total phenolic content and an opposite trend in antioxidant power (assessed by DPPH and FRAP assays) were observed. A total of 37 volatile substances were characterized in the aged flower-flavored wines. The aging process led to a decline in fruity and floral odors. Among the 12 month-aged wines, 1% (w/v) O. fragrans-flavored Chardonnay wine aged for 12 months was perceived as the most-liked product in human sensory analysis. This study manifested a bright future of edible flowers as a novel additive in the development of flavored wine with desirable sensory attributes. [ABSTRACT FROM AUTHOR]

Graphs that are critical (minimal excluded minors) for embeddability in surfaces are studied. In Part I, it was shown that graphs that are critical for embeddings into surfaces of Euler genus k or for embeddings into nonorientable surface of genus k are built from 3-connected components, called hoppers and cascades. In Part II, all cascades for Euler genus 2 are classified. As a consequence, the complete list of obstructions of connectivity 2 for embedding graphs into the Klein bottle is obtained. [ABSTRACT FROM AUTHOR]

We resolve the question of the existence of a finite 2‐complex with the same fundamental group and Euler characteristic as a Klein bottle with a bubble, but homotopically distinct to it. [ABSTRACT FROM AUTHOR]

In this work, the oxygen barrier property of polyethylene terephthalate (PET) films was enhanced by optimizing the "active" and "passive" synergistic barrier approaches, that is, integration of high barrier material polyethylene naphthalate (PEN) and oxygen scavengers along with catalyst with PET before biaxially stretching the PET blended films. When the PEN content in the PET blended films reached 50 wt%, the oxygen permeability coefficient was significantly reduced to 0.9687 cc·mil·m−2·day−1·0.1 MPa−1, showing an enhancement of 117.4 times compared to the pure PET film. Furthermore, the water vapor permeability coefficient was reduced to 8.0208 g·mil·m−2·day−1, representing a 45.5% reduction in comparison to the pristine PET film. It also maintained a higher transverse and longitudinal tensile strength (110.5 and 127.2 MPa) than PET film only with oxygen scavengers and catalyst (53.5 and 78.0 MPa). This work presents a viable and practical approach to endow PET materials with simultaneously enhanced oxygen, water barrier performance, and mechanical property. Highlights: The OPC of PET film was as low as 0.9687 cc·mil·m−2·day−1·0.1 MPa−1.The "active" and "passive" barrier techniques together reduced the OPC of PET film. [ABSTRACT FROM AUTHOR]

On the market for quality wine, quality is generally defined by the liquid's connection to a time, space, and person(s) of origin. This connection is achieved through material-discursive versions of terroir. While the consequences of this aesthetic regime have been well-studied, little attention has been given to the artefact through which the connection is enacted: the bottle. This paper, based on fieldwork with small-scale wine producers in Italy, asks what difference it makes for these artisanal producers to bottle their wine themselves. The analysis hones in on the bottle as a container embedded within an infrastructure of containment, and emphasises how the bottle as infrastructure re-orders the space–time of circulation. The result has implications for container-mediated processes of production-exchange-consumption everywhere. [ABSTRACT FROM AUTHOR]

Abstract Bottles and teats are ubiquitously used for feeding infants and young children. Yet there are limited empirical studies on the scope of infant feeding bottles, their attributes, or their marketing claims. We report the first comprehensive survey on infant feeding bottles and teats in Germany. We aimed to explore the extent of bottles and teats available in Germany, describe their physical attributes and analyze their marketing claims. A cross‐sectional survey of German bottle and teat manufacturer websites was conducted between June and November 2022. Product attributes are presented with descriptive statistics and photographs. Marketing claims are summarized in a descriptive content analysis. We identified 41 brands encompassing 447 unique products (226 bottles, 221 teats). The majority of bottles were plastic (147, 65%) or glass (64, 28%), and the majority of teats were silicone (188, 85%). Most brands (38, 93%) promoted products using one or more inappropriate marketing claims, including equivalency to breastfeeding (29, 73%), idealization through technical or medical descriptions (23, 58%), claims on disease prevention (31, 78%), references to naturalness (29, 73%), infant autonomy (10, 25%), and endorsements from parents (10, 25%) or health professionals (11, 28%). The majority of bottles and teats available in Germany appear to be marketed inappropriately and hold the potential to undermine public health recommendations on infant and young child feeding. Therefore, we recommend Germany strengthens legislation on the marketing of bottles and teats in accordance with the International Code of Marketing of Breastmilk Substitutes.

Macrocyclic hosts, such as cucurbit[8]uril (CB8), can significantly influence the outcomes of chemical reactions involving encapsulated reactive guests. In this study, we demonstrate that CB8 completely reverses the stereoselectivity of intramolecular [2+2] photo-cycloaddition reactions. Notably, it was also found that CB8 can trigger the unreactive diene to be reactive. [ABSTRACT FROM AUTHOR]

Antimicrobial polymers are a promising alternative to conventional antibiotics in the fight against antimicrobial resistance. Cationic bottle brush copolymers have shown to be superior to linear topologies in previous studies. Herein, the aspect ratio of such polymers is varied creating differently shaped confined unimolecular structures with varying degrees of side chain mobility. Using reversible addition‐fragmentation chain‐transfer (RAFT) polymerization, bottle brushes are produced in a one‐pot procedure. The morphology is confirmed by atomic force microscopy. The hydrophobicity, as determined via high performance‐liquid chromatography (HPLC) analysis, is drastically influenced by the topology. Using Fourier‐transform infrared (FTIR) spectroscopy, it is found that polymers with a high side chain mobility and increased global hydrophilicity, are less hydrated, and have stronger intramolecular hydrogen bonds. A phase segregated morphology leading to unimolecular micellization is assumed. Biological tests reveal increased antimicrobial activity for such segregated polymers. Their excellent hemocompatibility results in highly selective antimicrobial polymers whose adaptability seems to be a key feature in their excellent performance. This study highlights the tremendous importance of structural control in antimicrobial polymers. [ABSTRACT FROM AUTHOR]

Endoscopy is widely used to remove gastric foreign bodies using grasping forceps and loop snares to manipulate and retrieve the foreign material. However, as not all foreign bodies can be removed using conventional endoscopic techniques, this case series describes the use of bottle liners for the removal of gastric foreign bodies in 12 dogs between 2020 and 2023. A 4 oz bottle liner (Drop-Ins) was placed at the end of the endoscope and pushed into the stomach. With the help of forceps introduced into the operating channel, the foreign body was pushed into the bottle liner. The bottle liner containing the foreign body was then extracted with the help of traction threads. The technique was used as the initial retrieval method in 5 dogs because of smooth (i.e., difficult to grasp) or sharp (i.e., that may damage the digestive tract mucosa during removal) foreign bodies and as a rescue procedure in 7 dogs. Foreign body retrieval was successful in all 12 dogs, with minor complications reported in 5 dogs (erosions and bleeding of the gastroesophageal sphincter). The use of a bottle liner represents an affordable alternative to gastrotomy when foreign bodies cannot be grasped with forceps or snares. [ABSTRACT FROM AUTHOR]

In this study, we show that core/shell (CS) microgels with multiple layers can be created via a one-pot precipitation polymerization, in which monomers are added to the reaction flask multiple times once most of the previous monomer has been consumed. The complex internal structures of the microgels were examined using a combination of scattering and microscopy techniques. [ABSTRACT FROM AUTHOR]

Background: Conventional methods for isolation and antimicrobial susceptibility testing of bacterial isolates from positive blood culture bottles takes at least 48 hours. Direct antimicrobial susceptibility testing (AST) from positively flagged blood culture bottles helps to reduce the turnaround time (TAT) by 24 hours which will be useful in early initiation of appropriate antimicrobial therapy to reduce morbidity and mortality. Aims and Objectives: To evaluate the performance of direct AST from positively flagged BACTEC blood culture bottles and its comparison to conventional AST. Methods: A total of 356 blood culture bottles that were positively flagged on BACTEC 9120 were processed. Direct AST was performed from positive blood culture bottles. Bacterial isolates were identified using standard microbiological methods and tested against a wide spectrum of antimicrobial agents using the Kirby Bauer's disc diffusion method following the Clinical & Laboratory Standards Institute (CLSI) guidelines. Direct and conventional AST results were compared and Categorical agreement (CA) with various errors was evaluated. Results: On direct Gram staining, 107 samples showed Gram negative bacilli and 105 showed Gram positive cocci and 144 samples showed growth of contaminants. A total of 1486 organism-antibiotic combinations were evaluated, out of which 1438 (96.76%) combinations showed CA which was extremely satisfactory. The categorical disagreement was found only in 48 (3.23%) of organismantibiotic combinations; out of which major error was 23 (1.54%) followed by minor error 17 (1.14%) and very major error 8 (0.53%). Conclusions: The present study showed good concordance between the two methods and suggests the use of direct AST to reduce TAT by 24 hours for early initiation of therapy in patients with blood stream infections. [ABSTRACT FROM AUTHOR]

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The recycling and proper management of PET bottles is needed to avoid plastic pollution, as well as to achieve a plastic circular economy. In this study, we examined the recycling system of PET bottles and their material flows by life cycle, from production to recycling and disposal under different scenarios with reduced consumption for PET bottles. In Korea, PET bottle recycling has been managed by the extended producer responsibility system since 2003. As of 2021, annual usage of PET bottles per capita in Korea was estimated to be approximately 6.5 kg. The recycling rate of PET bottles after collection was determined to be 79% in 2021. However, recycled PET (or r-PET) from mechanical recycling is mostly used in low-grade PET applications such as short fibers, bands, and sheets. More than 112 thousand tons of r-PET chips would be needed to meet the 30% recycled content requirement in PET bottles in 2030 by regulation. Thus, there is an urgent need for high-quality secondary raw materials (i.e., food-grade) by adopting advanced sorting and recycling technologies for PET bottles with recycled content. PET bottle-to-bottle recycling practices can ensure the production of high-quality recycled materials and move Korea toward a plastic circular economy. [ABSTRACT FROM AUTHOR]

Graphs that are critical (minimal excluded minors) for embeddability in surfaces are studied. In Part I we consider the structure of graphs with a 2-vertex-cut that are critical with respect to the Euler genus. A general theorem describing the building blocks is presented. These constituents, called hoppers and cascades, are classified for the case when Euler genus is small. As a consequence, the complete list of obstructions of connectivity 2 for embedding graphs into the Klein bottle is obtained. This is the first complete result about obstructions for embeddability of graphs in the Klein bottle, and the outcome is somewhat surprising in the sense that there are considerably fewer excluded minors than expected. [ABSTRACT FROM AUTHOR]