45 results on '"Borselli C"'
Search Results
2. Comparative analysis of chondrocytes oxygen consumption in agarose and collagen gels
- Author
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GUACCIO A, BORSELLI C, NETTI, PAOLO ANTONIO, Guaccio, A, Borselli, C, and Netti, PAOLO ANTONIO
- Published
- 2008
3. Antiangiogenetic activity of the endocannabinoid anandamide.Correlation to its tumour-suppressor efficacy
- Author
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M Pisanti S, Borselli C., Gazzerro P, Laezza C, Bifulco. Pisanti S, Borselli C, Oliviero O, and Bifulco M.
- Published
- 2007
4. Anandamide inhibits endothelial cell proliferation in vitro and angiogenesis in vivo
- Author
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Pisanti S, Gazzerro P, Grimaldi C, Malfitano AM, Santoro A, Borselli C, Laezza C, Bifulco M., SARNATARO, DANIELA, Pisanti, S, Gazzerro, P, Grimaldi, C, Malfitano, Am, Santoro, A, Sarnataro, Daniela, Borselli, C, Laezza, C, and Bifulco, M.
- Published
- 2006
5. The spatial distribution and the temporal evolution of the matrix regulate cell function
- Author
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Borselli C, Oliviero O., De Rosa E., Battista S., Ambrosio L., and Netti P.A
- Subjects
collagen ,vascularization ,tissue engineering ,hyaluronic acid ,composite - Published
- 2006
6. Matrix-bound angiogenic factors enhance the angiogenic response within 3D biopolymers
- Author
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Borselli C, Oliviero O., Battista S., Ambrosio L., and Netti P.A.
- Subjects
collagen ,angiogenesis ,tissue engineering ,hyaluronic acid ,VEGF - Published
- 2005
7. Gradients of ECM control directional sprouting angiogenesis
- Author
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Borselli C., Oliviero O., Battista S., Ambrosio L., and Netti P.A
- Subjects
collagen ,angiogenesis ,tissue engineering ,extracellular matrix ,hyaluronic acid - Published
- 2005
8. Prevenzione delle aderenze intraddominali: recenti acquisizioni
- Author
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Breghi, Gloria, Borselli, C, and Duane, R.
- Published
- 1994
9. Human papilloma virus 16 E7 oncogene does not cooperate with RET/PTC 3 oncogene in the neoplastic transformation of thyroid cells in transgenic mice.
- Author
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Portella, Giuseppe, Borselli, C, Santoro, Massimo, Gerbasio, D, D'Armiento, M R, Dumont, Jacques Emile, Ledent, Catherine, Rothstein, Jay, Vecchio, G, Fusco, A., Portella, Giuseppe, Borselli, C, Santoro, Massimo, Gerbasio, D, D'Armiento, M R, Dumont, Jacques Emile, Ledent, Catherine, Rothstein, Jay, Vecchio, G, and Fusco, A.
- Abstract
We have previously reported that the thyroid-targeted expression of the RET/PTC3 oncogene (Tg-RET/PTC3) in transgenic mice induces follicular hyperplasia with papillary architecture, resulting in a modest increase of the thyroid gland volume, followed by the appearance of papillary carcinomas in approximately 1-year-old animals. In order to analyze the genetic alterations that may cooperate with RET/PTC3 in the development or progression of thyroid tumors, we interbred Tg-RET/PTC3 mice with Tg-E7 transgenic mice, which express the E7 oncogene of the human papilloma virus 16 in thyroid cells. Tg-E7 mice develop large colloid goiters with small papillae and well-differentiated thyroid carcinomas in older animals. Here we show that thyroid lesions in Tg-RET/PTC3-Tg-E7 double transgenics were morphologically different from those occurring in Tg-RET/PTC3 mice, while they were virtually indistinguishable from those occurring in Tg-E7 mice. In addition, the coexpression of RET/PTC3 and E7 oncogenes neither enhanced the malignant phenotype nor reduced the latency period of thyroid lesions with respect to parental transgenic lines. We conclude that the coexpression of RET/PTC3 and E7 lacks any cooperative effect in the neoplastic transformation of thyroid cells and that the E7-induced thyroid phenotype is dominant with respect to the RET/PTC3 one., Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S., info:eu-repo/semantics/published
- Published
- 2001
10. Time and Space Evolution of Transport Properties in Agarose–Chondrocyte Constructs
- Author
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Rosa, E. De, primary, Urciuolo, F., additional, Borselli, C., additional, Gerbasio, D., additional, Imparato, G., additional, and Netti, P.A., additional
- Published
- 2006
- Full Text
- View/download PDF
11. Transport of large molecules in hyaluronic acid-based membranes and solution
- Author
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DEROSA, E, primary, BORSELLI, C, additional, and NETTI, P, additional
- Published
- 2006
- Full Text
- View/download PDF
12. Mo-W12:5 Anadamide inhibits endothelial cell proliferation in vitro and angiogenesis in vivo
- Author
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Pisanti, S., primary, Gazzerro, P., additional, Grimaldi, C., additional, Mallitano, A.M., additional, Santoro, A., additional, Sarnataro, D., additional, Borselli, C., additional, Laezza, C., additional, and Bifulco, M., additional
- Published
- 2006
- Full Text
- View/download PDF
13. Human Papilloma Virus 16 E7 Oncogene Does Not Cooperate With RET/PTC 3 Oncogene in the Neoplastic Transformation of Thyroid Cells in Transgenic Mice
- Author
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Portella, G., primary, Borselli, C., additional, Santoro, M., additional, Gerbasio, D., additional, D'Armiento, M.R., additional, Dumont, J.E., additional, Ledent, C., additional, Rothstein, J.L., additional, Vecchio, G., additional, and Fusco, A., additional
- Published
- 2001
- Full Text
- View/download PDF
14. Functional muscle regeneration with combined delivery of angiogenesis and myogenesis factors
- Author
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Borselli, C., Storrie, H., Benesch-Lee, F., Shvartsman, Dmitry, Cezar, Christine Anne, Lichtman, Jeff, Vandenburgh, H. H., and Mooney, David J.
- Abstract
Regenerative efforts typically focus on the delivery of single factors, but it is likely that multiple factors regulating distinct aspects of the regenerative process (e.g., vascularization and stem cell activation) can be used in parallel to affect regeneration of functional tissues. This possibility was addressed in the context of ischemic muscle injury, which typically leads to necrosis and loss of tissue and function. The role of sustained delivery, via injectable gel, of a combination of VEGF to promote angiogenesis and insulin-like growth factor-1 (IGF1) to directly promote muscle regeneration and the return of muscle function in ischemic rodent hindlimbs was investigated. Sustained VEGF delivery alone led to neoangiogenesis in ischemic limbs, with complete return of tissue perfusion to normal levels by 3 weeks, as well as protection from hypoxia and tissue necrosis, leading to an improvement in muscle contractility. Sustained IGF1 delivery alone was found to enhance muscle fiber regeneration and protected cells from apoptosis. However, the combined delivery of VEGF and IGF1 led to parallel angiogenesis, reinnervation, and myogenesis; as satellite cell activation and proliferation was stimulated, cells were protected from apoptosis, the inflammatory response was muted, and highly functional muscle tissue was formed. In contrast, bolus delivery of factors did not have any benefit in terms of neoangiogenesis and perfusion and had minimal effect on muscle regeneration. These results support the utility of simultaneously targeting distinct aspects of the regenerative process., Molecular and Cellular Biology
- Published
- 2009
- Full Text
- View/download PDF
15. Biomimetic structures for implants and tissue engineering scaffolds
- Author
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Ambrosio, L., Battista, S., Borzacchiello, A., Borselli, C., Causa, F., Santis, R., PAOLO NETTI, Zeppetelli, S., Ambrosio, L, Battista, S., Borzacchiello, A., Borselli, C., Causa, Filippo, De Santis, R., Netti, PAOLO ANTONIO, and Zeppetelli, S.
- Subjects
Medicine (all)
16. Material guidance for cell metabolism in 3D in vitro cultures
- Author
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Guaccio, A., Borselli, C., Oliviero, O., PAOLO NETTI, Guaccio, Angela, Borselli, Cristina, Oliviero, Olimpia, and Netti, PAOLO ANTONIO
- Subjects
Biomaterials
17. Polymer composites as scaffolds for tissue engineering
- Author
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Ambrosio, L., Battista, S., Borzacchiello, A., Borselli, C., Causa, F., Santis, R., PAOLO NETTI, Nicolais, L., and Zeppetelli, S.
- Subjects
tissue engineering ,scaffolds ,composite ,hard tissue regeneration ,soft tissue regeneration
18. Human N-ras, TRK-Tl,smd RET/PTC3. oncogenes, driven by a thyroglobulin promoter, differently affect the expression of differentiation markers and the proliferation of thyroid epithelial cells
- Author
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Portella, G., Vitagliano, D., Borselli, C., Melillo, R. M., Salvatore, D., Rothstein, J. L., Vecchio, G., Alfredo Fusco, and Santoro, M.
19. Cellular mechanosensory: tissue engineering material design
- Author
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Borselli, C., primary, Vento, M.T., additional, Zeppetelli, S., additional, Ambrosio, L., additional, and Netti, P.A., additional
- Full Text
- View/download PDF
20. Cellular mechanosensory: tissue engineering material design.
- Author
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Borselli, C., Vento, M.T., Zeppetelli, S., Ambrosio, L., and Netti, P.A.
- Published
- 2002
- Full Text
- View/download PDF
21. Collagen_hyaluronic acid matrices:Effect on angiogenic sprouting
- Author
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Borselli, C., Oliviero, O., and Netti, P.A.
- Published
- 2006
- Full Text
- View/download PDF
22. Time and space evolution of transport properties in agarose-chondrocyte constructs
- Author
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Diego Gerbasio, E. De Rosa, Paolo A. Netti, Francesco Urciuolo, Giorgia Imparato, Cristina Borselli, DE ROSA, E, Urciuolo, F, Borselli, C, Gerbasio, D, Imparato, G, Netti, PAOLO ANTONIO, De Rosa, E., Urciuolo, F., Borselli, C., Gerbasio, Diego, Imparato, G., and Netti, P. A.
- Subjects
Male ,Chondrocyte ,Tissue Culture Techniques ,Diffusion ,Extracellular matrix ,chemistry.chemical_compound ,Chondrocytes ,Engineering (all) ,medicine ,Animals ,Bovine serum albumin ,Cells, Cultured ,Glycosaminoglycans ,biology ,Animal ,Chemistry ,Sepharose ,Cartilage ,General Engineering ,Fluid transport ,Photobleaching ,Dextran ,medicine.anatomical_structure ,Glycosaminoglycan ,biology.protein ,Biophysics ,Agarose ,Cattle ,Tissue Culture Technique ,Biomedical engineering - Abstract
During the development of de novo synthesized cartilage tissue engineered constructs, transport and biophysical properties are expected to change in time and space. Monitoring and control of the evolution of these parameters are of crucial importance to process biohybrid constructs in vitro. The aim of this work was to measure fluid and macromolecular transport and evolution of mechanical properties of tissue-engineered cartilage constructs as a function of culture time and extracellular matrix (ECM) production. It was found, in agreement with other literature reports, that mechanical and fluid transport properties of the constructs correlated well with time of culture and glycosaminoglycan (GAG) content. Further, diffusion coefficients of 2 probes, dextran (500 kDa) and bovine serum albumin (BSA), correlated well with GAG production. Diffusion coefficients (D) were measured with high spatial and temporal resolution by fluorescent recovery after photobleaching (FRAP). Diffusivity steadily decreases with time while it does not vary through the thickness of the specimen. On the basis of these results, an empirical relationship between diffusion coefficient and GAG content was proposed for the 2 probes analyzed. The results of this study provide useful information to optimize and control the tissue culture process in vitro.
- Published
- 2006
23. Human papilloma virus 16 E7 oncogene does not cooperate with RET/PTC 3 oncogene in the neoplastic transformation of thyroid cells in transgenic mice
- Author
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Diego Gerbasio, Cristina Borselli, Massimo Santoro, Catherine Ledent, Maria Rosaria D'Armiento, Jay L. Rothstein, Giancarlo Vecchio, Giuseppe Portella, Alfredo Fusco, Jacques Emile Dumont, Portella, Giuseppe, Borselli, C, Santoro, Massimo, Gerbasio, D, D'Armiento, Maria, Dumont, Je, Ledent, C, Rothstein, Jl, Vecchio, G, Fusco, Alfredo, Borselli, C., Gerbasio, D., D'Armiento, M. R., Dumont, E. J., Ledent, C., Rothstein, J. L., and Vecchio, Giancarlo
- Subjects
Male ,Genetically modified mouse ,endocrine system ,Cancer Research ,Pathology ,medicine.medical_specialty ,Time Factors ,endocrine system diseases ,Papillomavirus E7 Proteins ,Nuclear Receptor Coactivators ,Thyroid Gland ,Mice, Transgenic ,carcinoma ,Biology ,medicine.disease_cause ,thyroid ,Malignant transformation ,Thyroid carcinoma ,Mice ,oncogene ,medicine ,Animals ,Humans ,Neoplastic transformation ,Thyroid Neoplasms ,music ,Oncogene Proteins ,music.instrument ,Oncogene ,Goiter ,Homozygote ,Thyroid ,Age Factors ,Oncogene Proteins, Viral ,General Medicine ,Cell Transformation, Viral ,Follicular hyperplasia ,Carcinoma, Papillary ,Cell Transformation, Neoplastic ,Phenotype ,medicine.anatomical_structure ,Oncology ,Cancer research ,Female ,Carcinogenesis ,Cell Division ,Transcription Factors - Abstract
We have previously reported that the thyroid-targeted expression of the RET/PTC3 oncogene (Tg-RET/PTC3) in transgenic mice induces follicular hyperplasia with papillary architecture, resulting in a modest increase of the thyroid gland volume, followed by the appearance of papillary carcinomas in approximately 1-year-old animals. In order to analyze the genetic alterations that may cooperate with RET/PTC3 in the development or progression of thyroid tumors, we interbred Tg-RET/PTC3 mice with Tg-E7 transgenic mice, which express the E7 oncogene of the human papilloma virus 16 in thyroid cells. Tg-E7 mice develop large colloid goiters with small papillae and well-differentiated thyroid carcinomas in older animals. Here we show that thyroid lesions in Tg-RET/PTC3-Tg-E7 double transgenics were morphologically different from those occurring in Tg-RET/PTC3 mice, while they were virtually indistinguishable from those occurring in Tg-E7 mice. In addition, the coexpression of RET/PTC3 and E7 oncogenes neither enhanced the malignant phenotype nor reduced the latency period of thyroid lesions with respect to parental transgenic lines. We conclude that the coexpression of RET/PTC3 and E7 lacks any cooperative effect in the neoplastic transformation of thyroid cells and that the E7-induced thyroid phenotype is dominant with respect to the RET/PTC3 one.
24. Transport of large molecules in hyaluronic acid-based membranes and solution
- Author
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Cristina Borselli, Paolo A. Netti, Enrica De Rosa, DE ROSA, E, Borselli, C, and Netti, PAOLO ANTONIO
- Subjects
chemistry.chemical_classification ,Chemistry ,Hyaluronic acid ,Filtration and Separation ,Polymer ,engineering.material ,Permeation ,Molecular sieve ,Biochemistry ,chemistry.chemical_compound ,Membrane ,Chemical engineering ,Hindrance ,Drug delivery ,Polymer chemistry ,engineering ,Probes ,General Materials Science ,Collagen ,Biopolymer ,Physical and Theoretical Chemistry ,Molecular transport ,Macromolecule - Abstract
The aim of this work was to analyse the hindrance effect of the naturally derived bioactive polymer hyaluronic acid (HA) considered responsible for the interstitium transport regulation. The diffusion coefficient measurements of several macromolecular probes, different for nature and dimension have been performed within HA water solution and HA collagenous gels in order to study the role of HA on the control of macromolecular transport. HA matrices showed less diffusional hindrance then HA water solutions. Moreover, the HA, interpenetrating with the collagen fibres, selectively affected the transport, depending on the HA concentration. These preliminary studies indicate that the biopolymer HA can be employed to design novel biopolymer interpenetrating gels suitable as molecular sieves for membrane permeation and controlled drug delivery.
- Published
- 2006
25. Bioactivation of collagen matrices through sustained VEGF release from PLGA microspheres
- Author
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Olimpia Oliviero, Paolo A. Netti, Ivana d'Angelo, Fabiana Quaglia, Maria Immacolata La Rotonda, Cristina Borselli, Francesca Ungaro, Borselli, C, Ungaro, Francesca, Oliviero, Olimpia, D'Angelo, I, Quaglia, Fabiana, LA ROTONDA, MARIA IMMACOLATA, Netti, PAOLO ANTONIO, Ungaro, F, Oliviero, O, D'Angelo, Ivana, Quaglia, F, La Rotonda, Mi, and Netti, P. A.
- Subjects
Vascular Endothelial Growth Factor A ,Umbilical Veins ,Scaffold ,Materials science ,Endothelium ,Angiogenesis ,Biomedical Engineering ,Neovascularization, Physiologic ,bioactive scaffold ,Chick Embryo ,Chorioallantoic Membrane ,Fluorescence ,Biomaterials ,chemistry.chemical_compound ,Polylactic Acid-Polyglycolic Acid Copolymer ,Tissue engineering ,medicine ,Animals ,Humans ,Lactic Acid ,Microscopy, Confocal ,PLGA microsphere ,Temperature ,Metals and Alloys ,Endothelial Cells ,angiogenesi ,Hydrogen-Ion Concentration ,VEGF ,Microspheres ,Capillaries ,Cell biology ,Endothelial stem cell ,Vascular endothelial growth factor ,PLGA ,Vascular endothelial growth factor A ,medicine.anatomical_structure ,chemistry ,Ceramics and Composites ,Collagen ,Polyglycolic Acid ,Biomedical engineering - Abstract
The success of any tissue engineering implant relies upon prompt vascularization of the cellular construct and, hence, on the ability of the scaffold to broadcast specific activation of host endothelium and guide vessel ingrowth. Vascular endothelial growth factor (VEGF) is a potent angiogenic stimulator, and if released in a controlled manner it may enhance and guide scaffold vascularization. Therefore, the aim of this work was to realize a scaffold with integrated depots able to release VEGF in a controlled rate and assess the ability of this scaffold to promote angiogenesis. VEGF-loaded poly(lactide-co-glycolide) (PLGA) microspheres were produced and included in a collagen scaffold. The release of VEGF from microspheres was tailored to be sustained over several weeks and occurred at a rate of ∼0.6 ng/day per mg of microspheres. It was found that collagen scaffolds bioactivated with VEGF-loaded microspheres strongly enhanced endothelial cell activation and vascular sprouting both in vitro and in vivo as compared with a collagen scaffold bioactivated with free VEGF. This report demonstrates that by finely tuning VEGF release rate within a polymeric scaffold, sprouting of angiogenic vessels can be guided within the scaffolds interstices as well as broadcasted from the host tissues. © 2009 Wiley Periodicals, Inc.
- Published
- 2010
26. Antiangiogenic activity of the endocannabinoid anandamide. Correlation to its tumour-suppressor efficacy
- Author
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Cristina Borselli, Maurizio Bifulco, Olimpia Oliviero, Patrizia Gazzerro, Chiara Laezza, Simona Pisanti, Pisanti, S, Borselli, C, Oliviero, O, Laezza, C, Gazzerro, Patrizia, and Bifulco, Maurizio
- Subjects
medicine.medical_specialty ,Cannabinoid receptor ,Physiology ,Angiogenesis ,MAP Kinase Signaling System ,Polyunsaturated Alkamides ,Swine ,medicine.medical_treatment ,Clinical Biochemistry ,Neovascularization, Physiologic ,Angiogenesis Inhibitors ,Antineoplastic Agents ,Apoptosis ,Arachidonic Acids ,Chick Embryo ,Biology ,Matrix Metalloproteinase Inhibitors ,Chorioallantoic Membrane ,Neovascularization ,Receptor, Cannabinoid, CB1 ,Internal medicine ,Cell Line, Tumor ,Spheroids, Cellular ,Cannabinoid Receptor Modulators ,medicine ,Animals ,Humans ,Thyroid Neoplasms ,Cell Proliferation ,Tumor microenvironment ,Dose-Response Relationship, Drug ,Neovascularization, Pathologic ,Endothelial Cells ,Cell Differentiation ,Cell Biology ,Endocannabinoid system ,Coculture Techniques ,Cell biology ,Rats ,Endothelial stem cell ,Endocrinology ,Matrix Metalloproteinase 2 ,lipids (amino acids, peptides, and proteins) ,Fibroblast Growth Factor 2 ,Cannabinoid ,medicine.symptom ,Signal transduction ,Endocannabinoids - Abstract
Endocannabinoids are now emerging as suppressors of key cell-signaling pathways involved in cancer cell growth, invasion, and metastasis. We have previously observed that the metabolically stable anandamide analog, 2-methyl-2′-F-anandamide (Met-F-AEA) can inhibit the growth of thyroid cancer in vivo. Our hypothesis was that the anti-tumor effect observed could be at least in part ascribed to inhibition of neo-angiogenesis. Therefore, the aim of this study was to assess the anti-angiogenic activity of Met-F-AEA, to investigate the molecular mechanisms underlying this effect and whether Met-F-AEA could antagonize tumor-induced endothelial cell sprouting. We show that Met-F-AEA inhibited bFGF-stimulated endothelial cell proliferation, in a dose-dependent manner, and also induced apoptosis, both effects reliant on cannabinoid CB1 receptor stimulation. Analyzing the signaling pathways implicated in angiogenesis, we observed that the bFGF-induced ERK phosphorylation was antagonized by Met-F-AEA, and we found that p38 MAPK was involved in Met-F-AEA-induced apoptosis. Moreover, Met-F-AEA was able to inhibit bi-dimensional capillary-like tube formation and activity of matrix metalloprotease MMP-2, a major matrix degrading enzyme. Importantly, we demonstrated that Met-F-AEA is also functional in vivo since it inhibited angiogenesis in the chick chorioallantoic neovascularization model. Finally, Met-F-AEA inhibited tumor-induced angiogenesis in a three-dimensional model of endothelial and thyroid tumor cell (KiMol) spheroids co-cultures in different 3-D polymeric matrices that resemble tumor microenvironment and architecture. Thus, our results suggest that anandamide could be involved in the control of cancer growth targeting both tumor cell proliferation and the angiogenic stimulation of the vasculature. J. Cell. Physiol. 211: 495–503, 2007. © 2006 Wiley-Liss, Inc.
- Published
- 2007
27. Induction of directional sprouting angiogenesis by matrix gradients
- Author
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Olimpia Oliviero, Sabrina Battista, Paolo A. Netti, Luigi Ambrosio, Cristina Borselli, Borselli, C, Oliviero, O, Battista, S, Ambrosio, L, and Netti, PAOLO ANTONIO
- Subjects
Scaffold ,Materials science ,Biomedical Engineering ,Neovascularization, Physiologic ,Semi-interpenetrated network ,Biomaterials ,Matrix (mathematics) ,chemistry.chemical_compound ,Tissue engineering ,Collagen network ,Hyaluronic acid ,Animals ,Sprouting angiogenesi ,Hyaluronic Acid ,Sprouting angiogenesis ,Tissue Engineering ,Metals and Alloys ,Cell biology ,Culture Media ,Kinetics ,chemistry ,Tissue regeneration ,Ceramics and Composites ,Cattle ,Implant ,Collagen ,Sprouting ,Biomedical engineering - Abstract
The fate of any tissue engineering implant relies upon an adequate oxygen and nutrients supply throughout the cellular construct and, hence, by the ability of the scaffold to induce and guide vascular ingrowth. However, implant vascularization is usually an uncontrolled process that takes several weeks. In this work, we assessed the feasibility of controlling vascular sprout rate and direction within three-dimensional collagen–hyaluronic acid semi-interpenetrated networks by modulating the spatial distribution of the matricellular cues. Results indicated that increasing amount of hyaluronic acid (HA) within the matrix led to a progressive inhibition of sprouting. In HA-rich matrices, the sprout number and the propagation rate showed a 2.7- and 4-fold reduction, respectively, compared to collagen matrices. Furthermore, by creating HA gradients within the collagen network, we were able to direct and enhance the sprouting rate. This study provides an experimental platform for controlling vascularization of engineered tissues. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
- Published
- 2006
28. Mo-W12:5 Anadamide inhibits endothelial cell proliferation in vitro and angiogenesis in vivo
- Author
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Patrizia Gazzerro, C. Borselli, Antonietta Santoro, Claudia Grimaldi, A.M. Mallitano, D. Sarnataro, C. Laezza, Simona Pisanti, M. Bifulco, Pisanti, S., Gazzerro, P., Grimaldi, C., Mallitano, A. M., Santoro, A., Sarnataro, Daniela, Borselli, C., Laezza, C., and Bifulco, M.
- Subjects
Endothelial stem cell ,Vascular endothelial growth factor A ,Vasculogenesis ,Vascular endothelial growth factor C ,Angiogenesis ,Chemistry ,In vivo ,Internal Medicine ,General Medicine ,Cardiology and Cardiovascular Medicine ,In vitro ,Cell biology - Published
- 2006
29. The effect of matrix composition of 3D constructs on embryonic stem cell differentiation
- Author
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Sabrina Battista, Douglas R. Keene, Assunta Borzacchiello, S. Zeppetelli, Laura Mayol, Luigi Ambrosio, Cristina Borselli, Daniela Guarnieri, Diego Gerbasio, Paolo A. Netti, Battista, S, Guarnieri, D, Borselli, C, Zeppetelli, S, Borzacchiello, A, Mayol, Laura, Gerbasio, D, Keene, Dr, Ambrosio, L, and Netti, PAOLO ANTONIO
- Subjects
Cellular differentiation ,Cell Culture Techniques ,Biophysics ,Biocompatible Materials ,Bioengineering ,Embryoid body ,Endothelial cell differentiation ,Cell Line ,Biomaterials ,Mice ,fibronettina ,Tissue engineering ,Hardness ,Laminin ,Materials Testing ,Animals ,Extracellular Matrix Proteins ,collagene ,biology ,Stem Cells ,cellule staminali ,Cell Differentiation ,Embryonic stem cell ,Elasticity ,Extracellular Matrix ,Cell biology ,laminina ,Fibronectin ,Mechanics of Materials ,tissue engineering ,Immunology ,Ceramics and Composites ,biology.protein ,Stem cell - Abstract
The use of embryonic stem (ES) cells as unlimited cell source in tissue engineering has ignited the hope of regenerating any kind of tissue in vitro. However, the role of the material in control and guidance of their development and commitment into complex and viable three-dimensional (3D) tissues is still poorly understood. In this work, we investigate the role of material composition and structure on promoting ES cells growth and differentiation, by culturing mouse ES cell-derived embryoid bodies (EBs) in various semi-interpenetrating polymer networks (SIPNs), made of collagen, fibronectin (FN) and laminin (LM). We show that both composition and strength of the supportive matrix play an important role in EBs development. High collagen concentrations inhibit EBs cavitation and hence the following EBs differentiation, by inhibiting apoptosis. The presence of FN in 3D collagen constructs strongly stimulates endothelial cell differentiation and vascularization. Conversely, LM increases the ability of ES cells to differentiate into beating cardiomyocytes. Our data suggest that matrix composition has an important role in EBs development and that it is possible to influence stem cell differentiation toward preferential pattern, by modulating the physical and biochemical properties of the scaffold.
- Published
- 2005
30. Collagen_hyaluronic acid matrices:Effect on angiogenic sprouting
- Author
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Paolo A. Netti, Olimpia Oliviero, Cristina Borselli, Borselli, C, Oliviero, O, and Netti, PAOLO ANTONIO
- Subjects
Chemistry ,Biophysics ,Molecular Biology ,Sprouting - Published
- 2006
31. Apremilast as a Potential Targeted Therapy for Metabolic Syndrome in Patients with Psoriasis: An Observational Analysis.
- Author
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Campione E, Zarabian N, Cosio T, Borselli C, Artosi F, Cont R, Sorge R, Shumak RG, Costanza G, Rivieccio A, Gaziano R, and Bianchi L
- Abstract
Psoriasis (PsO) is a chronic inflammatory dermatosis that often presents with erythematous, sharply demarcated lesions. Although psoriasis is primarily a dermatological disease, its immune-mediated pathogenesis produces systemic effects and is closely associated with various comorbid conditions such as cardiovascular disease (CVD), metabolic syndrome (MetS), and diabetes mellitus type II (DMII). Apremilast, an oral phosphodiesterase 4 (PDE-4) inhibitor, has shown promise in treating moderate-to-severe psoriasis and is associated with potential cardiometabolic benefits. In a 12-month prospective observational study involving 137 patients with moderate-to-severe psoriasis, we assessed changes in psoriasis clinimetric scores and metabolic profiles from baseline (T0) to 52 weeks (T1) to evaluate the efficacy of apremilast. After 52 weeks of apremilast treatment, we documented a statistically significant decrease in low-density lipoprotein (LDL) and total cholesterol, triglycerides, and glucose levels. Our findings even suggest a potential synergistic effect among patients treated with apremilast, alongside concomitant statin and/or insulin therapy. Although the results of our study must be validated on a larger scale, the use of apremilast in the treatment of psoriatic patients with cardio-metabolic comorbidities yields promising results.
- Published
- 2024
- Full Text
- View/download PDF
32. A Real-Life Study on the Use of Tildrakizumab in Psoriatic Patients.
- Author
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Campione E, Lambiase S, Gaeta Shumak R, Galluzzo M, Lanna C, Costanza G, Borselli C, Artosi F, Cosio T, Tofani L, Dattola A, Di Daniele F, and Bianchi L
- Abstract
Tildrakizumab is a humanized IgG1κ monoclonal antibody that selectively targets the p19 subunit of interleukin IL-23, thereby inhibiting the IL-23/IL-17 axis, which is primarily implicated in the immunopathogenesis of psoriasis. Tildrakizumab is approved for the treatment of moderate-to-severe plaque-type psoriasis in adults based on the evidence of two randomized and controlled phase-III clinical trials (reSURFACE 1 and reSURFACE 2). Here, we report our real-life experience treating 53 psoriatic patients (19 female and 34 male) who were administered tildrakizumab every 12 weeks and received follow-ups over 52 weeks. Descriptive and inferential statistical analyses were performed, in particular the Psoriasis Area and Severity Index (PASI), Dermatology Life Quality Index (DLQI) and, if applicable, the Nail Psoriasis Severity Index (NAPSI) and Palmoplantar Psoriasis Physician Global Assessment (PPPGA). These were assessed at baseline and after different timepoints (weeks) during the follow-up period. We described and evaluated demographical and epidemiological characteristics in our cohort group, focusing on comorbidities. In this group, 35.9% of patients were female and 64.1% were male, with 47.1% being smokers and with a mean age of 51.2 years. A total of 37.7% of these patients was affected by scalp psoriasis; regarding comorbidities, hypertension was the most frequent (32.5%), followed by psoriatic arthritis (PsA) (18.60%) and diabetes (13.9%). At week 52, 93%, 90.2% and 77% of patients achieved a PASI reduction ≥75% (PASI 75), PASI 90 and PASI 100, respectively. In addition, NAPSI, PPPGA and DLQI scores were significantly reduced by week 52. In our cohort of complex psoriasis patients, disease remission began at the end of the fourth week of treatment and remained constant from week 16 to week 52.
- Published
- 2023
- Full Text
- View/download PDF
33. Why targeted therapeutics have provided benefit in psoriasis: looking at IL-17 biology.
- Author
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Lanna C, Lambiase S, Gaeta Shumak R, Borselli C, Cosio T, Dattola A, Bianchi L, and Campione E
- Subjects
- Humans, Antibodies, Monoclonal pharmacology, Antibodies, Monoclonal therapeutic use, Cytokines metabolism, Cytokines therapeutic use, Biology, Interleukin-17, Psoriasis pathology
- Abstract
Introduction: Psoriasis is an inflammatory, chronic and immune-mediated disease that can affect the skin and joints. Pro-inflammatory cytokines have a dominant role in the pathogenesis of this heterogeneous disease in which the IL-23/IL-17 axis plays a crucial role. The IL-17 family is involved in numerous processes such as immune defense, intestinal disorders and diseases of the central nervous system. In psoriasis, in particular, many cytokines belonging to the IL-17 family are involved in the inflammatory cascade underlying the disease., Areas Covered: The knowledge of the mechanisms and pathways behind psoriasis is crucial for the development of new target therapies. We focused on IL-17 biology in order to understand why biological drugs against this cytokine are an effective treatment for moderate to severe psoriasis. Clinical trials results of ixekizumab, brodalumab, secukinumab and bimekizumab have been presented., Expert Opinion: Il-17 inhibitors are a very fast and effective treatment against psoriasis; however, fungal infections can occur during their use, due to IL-17 biological functions. Therefore, it should be mandatory to choose the right patients to treat with these monoclonal antibodies in order to have a tailored target therapy for each patient.
- Published
- 2022
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34. Synthetic niche to modulate regenerative potential of MSCs and enhance skeletal muscle regeneration.
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Pumberger M, Qazi TH, Ehrentraut MC, Textor M, Kueper J, Stoltenburg-Didinger G, Winkler T, von Roth P, Reinke S, Borselli C, Perka C, Mooney DJ, Duda GN, and Geißler S
- Subjects
- Animals, Apoptosis, Cell Culture Techniques, Cell Differentiation, Cell Line, Cell Movement, Cell Proliferation, Cell Survival, Female, Humans, Insulin-Like Growth Factor I metabolism, Mechanical Phenomena, Mesenchymal Stem Cell Transplantation, Mice, Muscle Development, Rats, Sprague-Dawley, Regeneration, Tissue Engineering, Vascular Endothelial Growth Factor A metabolism, Mesenchymal Stem Cells cytology, Muscle, Skeletal physiology, Wound Healing physiology
- Abstract
Severe injury to the skeletal muscle often results in the formation of scar tissue, leading to a decline in functional performance. Traditionally, tissue engineering strategies for muscle repair have focused on substrates that promote myogenic differentiation of transplanted cells. In the current study, the reported data indicates that mesenchymal stromal cells (MSCs) transplanted via porous alginate cryogels promote muscle regeneration by secreting bioactive factors that profoundly influence the function of muscle progenitor cells. These cellular functions, which include heightened resistance of muscle progenitor cells to apoptosis, migration to site of injury, and prevention of premature differentiation are highly desirable in the healing cascade after acute muscle trauma. Furthermore, stimulation of MSCs with recombinant growth factors IGF-1 and VEGF165 was found to significantly enhance their paracrine effects on muscle progenitor cells. Multifunctional alginate cryogels were then utilized as synthetic niches that facilitate local stimulation of seeded MSCs by providing a sustained release of growth factors. In a clinically relevant injury model, the modulation of MSC paracrine signaling via engineered niches significantly improved muscle function by remodeling scar tissue and promoting the formation of new myofibers, outperforming standalone cell or growth factor delivery., (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
35. Design and fabrication of a biodegradable, covalently crosslinked shape-memory alginate scaffold for cell and growth factor delivery.
- Author
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Wang L, Shansky J, Borselli C, Mooney D, and Vandenburgh H
- Subjects
- Animals, Cell Movement physiology, Cell Proliferation, Cell Survival physiology, Cells, Cultured, Glucuronic Acid chemistry, Hexuronic Acids chemistry, Intercellular Signaling Peptides and Proteins administration & dosage, Microscopy, Electron, Scanning, Alginates chemistry, Biocompatible Materials chemistry, Tissue Engineering methods, Tissue Scaffolds chemistry
- Abstract
The successful use of transplanted cells and/or growth factors for tissue repair is limited by a significant cell loss and/or rapid growth factor diffusion soon after implantation. Highly porous alginate scaffolds formed with covalent crosslinking have been used to improve cell survival and growth factor release kinetics, but require open-wound surgical procedures for insertion and have not previously been designed to readily degrade in vivo. In this study, a biodegradable, partially crosslinked alginate scaffold with shape-memory properties was fabricated for minimally invasive surgical applications. A mixture of high and low molecular weight partially oxidized alginate modified with RGD peptides was covalently crosslinked using carbodiimide chemistry. The scaffold was compressible 11-fold and returned to its original shape when rehydrated. Scaffold degradation properties in vitro indicated ~85% mass loss by 28 days. The greater than 90% porous scaffolds released the recombinant growth factor insulin-like growth factor-1 over several days in vitro and allowed skeletal muscle cell survival, proliferation, and migration from the scaffold over a 28-day period. The compressible scaffold thus has the potential to be delivered by a minimally invasive technique, and when rehydrated in vivo with cells and/or growth factors, could serve as a temporary delivery vehicle for tissue repair.
- Published
- 2012
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36. The role of multifunctional delivery scaffold in the ability of cultured myoblasts to promote muscle regeneration.
- Author
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Borselli C, Cezar CA, Shvartsman D, Vandenburgh HH, and Mooney DJ
- Subjects
- Alginates chemistry, Animals, Cells, Cultured, Female, Glucuronic Acid chemistry, Hexuronic Acids chemistry, Humans, Mice, Mice, Inbred C57BL, Mice, Transgenic, Insulin-Like Growth Factor I administration & dosage, Muscle, Skeletal physiology, Myoblasts cytology, Regeneration, Tissue Scaffolds chemistry, Vascular Endothelial Growth Factor A administration & dosage
- Abstract
Many cell types of therapeutic interest, including myoblasts, exhibit reduced engraftment if cultured prior to transplantation. This study investigated whether polymeric scaffolds that direct cultured myoblasts to migrate outwards and repopulate the host damaged tissue, in concert with release of angiogenic factors designed to enhance revascularizaton of the regenerating tissue, would enhance the efficacy of this cell therapy and lead to functional muscle regeneration. This was investigated in the context of a severe injury to skeletal muscle tissue involving both myotoxin-mediated direct damage and induction of regional ischemia. Local and sustained release of VEGF and IGF-1 from macroporous scaffolds used to transplant and disperse cultured myogenic cells significantly enhanced their engraftment, limited fibrosis, and accelerated the regenerative process. This resulted in increased muscle mass and, improved contractile function. These results demonstrate the importance of finely controlling the microenvironment of transplanted cells in the treatment of severe muscle damage., (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Published
- 2011
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37. Functional muscle regeneration with combined delivery of angiogenesis and myogenesis factors.
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Borselli C, Storrie H, Benesch-Lee F, Shvartsman D, Cezar C, Lichtman JW, Vandenburgh HH, and Mooney DJ
- Subjects
- Animals, Cell Proliferation, Female, Ischemia, Mice, Mice, Inbred C57BL, Muscle, Skeletal blood supply, Muscle, Skeletal drug effects, Muscular Diseases drug therapy, Muscular Diseases physiopathology, Myoblasts drug effects, Myoblasts physiology, Insulin-Like Growth Factor I administration & dosage, Muscle Development drug effects, Muscle, Skeletal physiology, Neovascularization, Physiologic drug effects, Regeneration drug effects, Vascular Endothelial Growth Factor A administration & dosage
- Abstract
Regenerative efforts typically focus on the delivery of single factors, but it is likely that multiple factors regulating distinct aspects of the regenerative process (e.g., vascularization and stem cell activation) can be used in parallel to affect regeneration of functional tissues. This possibility was addressed in the context of ischemic muscle injury, which typically leads to necrosis and loss of tissue and function. The role of sustained delivery, via injectable gel, of a combination of VEGF to promote angiogenesis and insulin-like growth factor-1 (IGF1) to directly promote muscle regeneration and the return of muscle function in ischemic rodent hindlimbs was investigated. Sustained VEGF delivery alone led to neoangiogenesis in ischemic limbs, with complete return of tissue perfusion to normal levels by 3 weeks, as well as protection from hypoxia and tissue necrosis, leading to an improvement in muscle contractility. Sustained IGF1 delivery alone was found to enhance muscle fiber regeneration and protected cells from apoptosis. However, the combined delivery of VEGF and IGF1 led to parallel angiogenesis, reinnervation, and myogenesis; as satellite cell activation and proliferation was stimulated, cells were protected from apoptosis, the inflammatory response was muted, and highly functional muscle tissue was formed. In contrast, bolus delivery of factors did not have any benefit in terms of neoangiogenesis and perfusion and had minimal effect on muscle regeneration. These results support the utility of simultaneously targeting distinct aspects of the regenerative process.
- Published
- 2010
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38. Bioactivation of collagen matrices through sustained VEGF release from PLGA microspheres.
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Borselli C, Ungaro F, Oliviero O, d'Angelo I, Quaglia F, La Rotonda MI, and Netti PA
- Subjects
- Animals, Capillaries drug effects, Capillaries metabolism, Chick Embryo, Chorioallantoic Membrane blood supply, Endothelial Cells cytology, Endothelial Cells drug effects, Fluorescence, Humans, Hydrogen-Ion Concentration, Microscopy, Confocal, Neovascularization, Physiologic drug effects, Polylactic Acid-Polyglycolic Acid Copolymer, Temperature, Umbilical Veins cytology, Collagen metabolism, Lactic Acid pharmacology, Microspheres, Polyglycolic Acid pharmacology, Vascular Endothelial Growth Factor A metabolism
- Abstract
The success of any tissue engineering implant relies upon prompt vascularization of the cellular construct and, hence, on the ability of the scaffold to broadcast specific activation of host endothelium and guide vessel ingrowth. Vascular endothelial growth factor (VEGF) is a potent angiogenic stimulator, and if released in a controlled manner it may enhance and guide scaffold vascularization. Therefore, the aim of this work was to realize a scaffold with integrated depots able to release VEGF in a controlled rate and assess the ability of this scaffold to promote angiogenesis. VEGF-loaded poly(lactide-co-glycolide) (PLGA) microspheres were produced and included in a collagen scaffold. The release of VEGF from microspheres was tailored to be sustained over several weeks and occurred at a rate of approximately 0.6 ng/day per mg of microspheres. It was found that collagen scaffolds bioactivated with VEGF-loaded microspheres strongly enhanced endothelial cell activation and vascular sprouting both in vitro and in vivo as compared with a collagen scaffold bioactivated with free VEGF. This report demonstrates that by finely tuning VEGF release rate within a polymeric scaffold, sprouting of angiogenic vessels can be guided within the scaffolds interstices as well as broadcasted from the host tissues.
- Published
- 2010
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39. Oxygen consumption of chondrocytes in agarose and collagen gels: a comparative analysis.
- Author
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Guaccio A, Borselli C, Oliviero O, and Netti PA
- Subjects
- Animals, Cattle, Cell Culture Techniques, Cell Survival, Cells, Cultured, Chondrocytes cytology, Gels, Glucose metabolism, Tissue Engineering methods, Chondrocytes metabolism, Collagen, Oxygen Consumption physiology, Sepharose
- Abstract
The growth of engineered cartilage tissue in vitro is often impaired by the problem of insufficient oxygen and nutrient supply to cells seeded in 3D constructs. Despite its central role in controlling most cell functions, the scaffolding material has generally been thought of only as a transport barrier and its potential active role in controlling oxygen uptake has never been addressed. In this work the role of cell-material interaction on oxygen metabolism in 3D in vitro cultures was surveyed. To this aim bovine chondrocytes, at a cell density of 400,000 and 4,000,000 cells/mL, respectively, were seeded in collagen type I and in agarose, while keeping all other culture conditions constant. A unidirectional oxygen gradient was induced in the culture through the application of a "sandwich" model and the oxygen concentration at the pericellular level was measured by phosphorescence quenching microscopy. Results show that the oxygen consumption rate is two-fold higher in agarose than in collagen, which indicates that the nature of the material strongly influences cell metabolic behaviour. Moreover, since different oxygen consumption rates are linked to different cell biosynthetic activity, our findings will prove beyond any doubt the active role played by materials in tissue regeneration.
- Published
- 2008
- Full Text
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40. Antiangiogenic activity of the endocannabinoid anandamide: correlation to its tumor-suppressor efficacy.
- Author
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Pisanti S, Borselli C, Oliviero O, Laezza C, Gazzerro P, and Bifulco M
- Subjects
- Angiogenesis Inhibitors therapeutic use, Animals, Antineoplastic Agents therapeutic use, Apoptosis drug effects, Arachidonic Acids therapeutic use, Cannabinoid Receptor Modulators therapeutic use, Cell Differentiation drug effects, Cell Line, Tumor, Cell Proliferation drug effects, Chick Embryo, Chorioallantoic Membrane blood supply, Chorioallantoic Membrane drug effects, Coculture Techniques, Dose-Response Relationship, Drug, Endothelial Cells cytology, Endothelial Cells metabolism, Fibroblast Growth Factor 2 pharmacology, Humans, MAP Kinase Signaling System drug effects, Matrix Metalloproteinase 2 metabolism, Matrix Metalloproteinase Inhibitors, Neovascularization, Pathologic metabolism, Polyunsaturated Alkamides therapeutic use, Rats, Receptor, Cannabinoid, CB1 metabolism, Spheroids, Cellular, Swine, Thyroid Neoplasms metabolism, Thyroid Neoplasms pathology, Angiogenesis Inhibitors pharmacology, Antineoplastic Agents pharmacology, Arachidonic Acids pharmacology, Cannabinoid Receptor Modulators pharmacology, Endocannabinoids, Endothelial Cells drug effects, Neovascularization, Pathologic prevention & control, Neovascularization, Physiologic drug effects, Polyunsaturated Alkamides pharmacology, Receptor, Cannabinoid, CB1 agonists
- Abstract
Endocannabinoids are now emerging as suppressors of key cell-signaling pathways involved in cancer cell growth, invasion, and metastasis. We have previously observed that the metabolically stable anandamide analog, 2-methyl-2'-F-anandamide (Met-F-AEA) can inhibit the growth of thyroid cancer in vivo. Our hypothesis was that the anti-tumor effect observed could be at least in part ascribed to inhibition of neo-angiogenesis. Therefore, the aim of this study was to assess the anti-angiogenic activity of Met-F-AEA, to investigate the molecular mechanisms underlying this effect and whether Met-F-AEA could antagonize tumor-induced endothelial cell sprouting. We show that Met-F-AEA inhibited bFGF-stimulated endothelial cell proliferation, in a dose-dependent manner, and also induced apoptosis, both effects reliant on cannabinoid CB1 receptor stimulation. Analyzing the signaling pathways implicated in angiogenesis, we observed that the bFGF-induced ERK phosphorylation was antagonized by Met-F-AEA, and we found that p38 MAPK was involved in Met-F-AEA-induced apoptosis. Moreover, Met-F-AEA was able to inhibit bi-dimensional capillary-like tube formation and activity of matrix metalloprotease MMP-2, a major matrix degrading enzyme. Importantly, we demonstrated that Met-F-AEA is also functional in vivo since it inhibited angiogenesis in the chick chorioallantoic neovascularization model. Finally, Met-F-AEA inhibited tumor-induced angiogenesis in a three-dimensional model of endothelial and thyroid tumor cell (KiMol) spheroids co-cultures in different 3-D polymeric matrices that resemble tumor microenvironment and architecture. Thus, our results suggest that anandamide could be involved in the control of cancer growth targeting both tumor cell proliferation and the angiogenic stimulation of the vasculature., ((c) 2007 Wiley-Liss, Inc.)
- Published
- 2007
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41. Induction of directional sprouting angiogenesis by matrix gradients.
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Borselli C, Oliviero O, Battista S, Ambrosio L, and Netti PA
- Subjects
- Animals, Cattle, Collagen, Culture Media, Hyaluronic Acid pharmacology, Kinetics, Neovascularization, Physiologic drug effects, Tissue Engineering methods
- Abstract
The fate of any tissue engineering implant relies upon an adequate oxygen and nutrients supply throughout the cellular construct and, hence, by the ability of the scaffold to induce and guide vascular ingrowth. However, implant vascularization is usually an uncontrolled process that takes several weeks. In this work, we assessed the feasibility of controlling vascular sprout rate and direction within three-dimensional collagen-hyaluronic acid semi-interpenetrated networks by modulating the spatial distribution of the matricellular cues. Results indicated that increasing amount of hyaluronic acid (HA) within the matrix led to a progressive inhibition of sprouting. In HA-rich matrices, the sprout number and the propagation rate showed a 2.7- and 4-fold reduction, respectively, compared to collagen matrices. Furthermore, by creating HA gradients within the collagen network, we were able to direct and enhance the sprouting rate. This study provides an experimental platform for controlling vascularization of engineered tissues., (Copyright 2006 Wiley Periodicals, Inc.)
- Published
- 2007
- Full Text
- View/download PDF
42. Time and space evolution of transport properties in agarose-chondrocyte constructs.
- Author
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De Rosa E, Urciuolo F, Borselli C, Gerbasio D, Imparato G, and Netti PA
- Subjects
- Animals, Cattle, Cells, Cultured, Diffusion, Glycosaminoglycans biosynthesis, Male, Chondrocytes metabolism, Sepharose, Tissue Culture Techniques
- Abstract
During the development of de novo synthesized cartilage tissue engineered constructs, transport and biophysical properties are expected to change in time and space. Monitoring and control of the evolution of these parameters are of crucial importance to process biohybrid constructs in vitro. The aim of this work was to measure fluid and macromolecular transport and evolution of mechanical properties of tissue-engineered cartilage constructs as a function of culture time and extracellular matrix (ECM) production. It was found, in agreement with other literature reports, that mechanical and fluid transport properties of the constructs correlated well with time of culture and glycosaminoglycan (GAG) content. Further, diffusion coefficients of 2 probes, dextran (500 kDa) and bovine serum albumin (BSA), correlated well with GAG production. Diffusion coefficients (D) were measured with high spatial and temporal resolution by fluorescent recovery after photobleaching (FRAP). Diffusivity steadily decreases with time while it does not vary through the thickness of the specimen. On the basis of these results, an empirical relationship between diffusion coefficient and GAG content was proposed for the 2 probes analyzed. The results of this study provide useful information to optimize and control the tissue culture process in vitro.
- Published
- 2006
- Full Text
- View/download PDF
43. The effect of matrix composition of 3D constructs on embryonic stem cell differentiation.
- Author
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Battista S, Guarnieri D, Borselli C, Zeppetelli S, Borzacchiello A, Mayol L, Gerbasio D, Keene DR, Ambrosio L, and Netti PA
- Subjects
- Animals, Biocompatible Materials chemistry, Cell Culture Techniques methods, Cell Line, Elasticity, Extracellular Matrix chemistry, Extracellular Matrix ultrastructure, Extracellular Matrix Proteins chemistry, Extracellular Matrix Proteins ultrastructure, Hardness, Materials Testing, Mice, Biocompatible Materials metabolism, Cell Differentiation physiology, Extracellular Matrix metabolism, Extracellular Matrix Proteins metabolism, Stem Cells cytology, Stem Cells physiology, Tissue Engineering methods
- Abstract
The use of embryonic stem (ES) cells as unlimited cell source in tissue engineering has ignited the hope of regenerating any kind of tissue in vitro. However, the role of the material in control and guidance of their development and commitment into complex and viable three-dimensional (3D) tissues is still poorly understood. In this work, we investigate the role of material composition and structure on promoting ES cells growth and differentiation, by culturing mouse ES cell-derived embryoid bodies (EBs) in various semi-interpenetrating polymer networks (SIPNs), made of collagen, fibronectin (FN) and laminin (LM). We show that both composition and strength of the supportive matrix play an important role in EBs development. High collagen concentrations inhibit EBs cavitation and hence the following EBs differentiation, by inhibiting apoptosis. The presence of FN in 3D collagen constructs strongly stimulates endothelial cell differentiation and vascularization. Conversely, LM increases the ability of ES cells to differentiate into beating cardiomyocytes. Our data suggest that matrix composition has an important role in EBs development and that it is possible to influence stem cell differentiation toward preferential pattern, by modulating the physical and biochemical properties of the scaffold.
- Published
- 2005
- Full Text
- View/download PDF
44. Biomimetic structures for implants and tissue engineering scaffolds.
- Author
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Ambrosio L, Battista S, Borzacchiello A, Borselli C, Causa F, De Santis R, Netti PA, and Zeppetelli S
- Subjects
- Biodegradation, Environmental, Bone Screws, Humans, Polylactic Acid-Polyglycolic Acid Copolymer, Structure-Activity Relationship, Biocompatible Materials, Lactic Acid analysis, Materials Testing, Polyglycolic Acid analysis, Polymers analysis, Tissue Engineering instrumentation
- Published
- 2004
45. Human N-ras, TRK-T1, and RET/PTC3 oncogenes, driven by a thyroglobulin promoter, differently affect the expression of differentiation markers and the proliferation of thyroid epithelial cells.
- Author
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Portella G, Vitagliano D, Borselli C, Melillo RM, Salvatore D, Rothstein JL, Vecchio G, Fusco A, and Santoro M
- Subjects
- Animals, Antigens, Differentiation biosynthesis, Cattle, Cell Differentiation, Cell Division genetics, Cell Transformation, Neoplastic, Cells, Cultured, Epithelial Cells cytology, Humans, Mice, Nuclear Receptor Coactivators, Phenotype, Rats, Rats, Inbred F344, Recombinant Fusion Proteins genetics, Transfection, Genes, ras, Oncogene Proteins genetics, Promoter Regions, Genetic, Thyroglobulin genetics, Thyroid Gland cytology, Transcription Factors
- Abstract
The ras family members and the tyrosine kinases RET and TRK are frequently activated in human tumors of the thyroid gland. To ascertain the effects of these oncogenes in cultured thyroid cells we have generated expression vectors containing activated versions of the three genes under the control of the thyroid-specific thyroglobulin gene promoter. Here we show that the expression of the three oncogenes differently affects thyroid differentiation. While the TRK-T1 oncogene interferes with the capability of thyroid cells of trapping iodide and only marginally affects thyroglobulin gene expression, both RET/PTC3 and N-ras(Gln61-Lys) induce a dramatic reduction of thyroglobulin mRNA and alleviate TSH dependency for cellular growth. However, none of the three oncogenes is able to induce the appearance of neoplastic transformation markers, such as growth in semisolid medium and tumorigenicity in athymic mice. This indicates that genetic events additional to TRK, RET, or N-ras activation are required for fully malignant transformation of thyroid cells.
- Published
- 1999
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