Back to Search
Start Over
The role of multifunctional delivery scaffold in the ability of cultured myoblasts to promote muscle regeneration.
- Source :
-
Biomaterials [Biomaterials] 2011 Dec; Vol. 32 (34), pp. 8905-14. Date of Electronic Publication: 2011 Sep 10. - Publication Year :
- 2011
-
Abstract
- Many cell types of therapeutic interest, including myoblasts, exhibit reduced engraftment if cultured prior to transplantation. This study investigated whether polymeric scaffolds that direct cultured myoblasts to migrate outwards and repopulate the host damaged tissue, in concert with release of angiogenic factors designed to enhance revascularizaton of the regenerating tissue, would enhance the efficacy of this cell therapy and lead to functional muscle regeneration. This was investigated in the context of a severe injury to skeletal muscle tissue involving both myotoxin-mediated direct damage and induction of regional ischemia. Local and sustained release of VEGF and IGF-1 from macroporous scaffolds used to transplant and disperse cultured myogenic cells significantly enhanced their engraftment, limited fibrosis, and accelerated the regenerative process. This resulted in increased muscle mass and, improved contractile function. These results demonstrate the importance of finely controlling the microenvironment of transplanted cells in the treatment of severe muscle damage.<br /> (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Subjects :
- Alginates chemistry
Animals
Cells, Cultured
Female
Glucuronic Acid chemistry
Hexuronic Acids chemistry
Humans
Mice
Mice, Inbred C57BL
Mice, Transgenic
Insulin-Like Growth Factor I administration & dosage
Muscle, Skeletal physiology
Myoblasts cytology
Regeneration
Tissue Scaffolds chemistry
Vascular Endothelial Growth Factor A administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1878-5905
- Volume :
- 32
- Issue :
- 34
- Database :
- MEDLINE
- Journal :
- Biomaterials
- Publication Type :
- Academic Journal
- Accession number :
- 21911253
- Full Text :
- https://doi.org/10.1016/j.biomaterials.2011.08.019