28 results on '"Borin, Daniele"'
Search Results
2. Altered microtubule structure, hemichannel localization and beating activity in cardiomyocytes expressing pathologic nuclear lamin A/C.
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Borin, Daniele, Peña, Brisa, Chen, Suet Nee, Long, Carlin S, Taylor, Matthew RG, Mestroni, Luisa, and Sbaizero, Orfeo
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Atomic force microscopy ,Beating ,Biological sciences ,Biomechanics ,Cardiology ,Cardiomyocytes ,Cardiomyopathy ,Cx43 ,Cytoskeleton ,Lamin A/C ,Mechanical property ,Membrane ,Heart Disease ,Bioengineering ,Cardiovascular ,2.1 Biological and endogenous factors - Abstract
Given the clinical effect of laminopathies, understanding lamin mechanical properties will benefit the treatment of heart failure. Here we report a mechano-dynamic study of LMNA mutations in neonatal rat ventricular myocytes (NRVM) using single cell spectroscopy with Atomic Force Microscopy (AFM) and measured changes in beating force, frequency and contractile amplitude of selected mutant-expressing cells within cell clusters. Furthermore, since beat-to-beat variations can provide clues on the origin of arrhythmias, we analyzed the beating rate variability using a time-domain method which provides a Poincaré plot. Data were further correlated to cell phenotypes. Immunofluorescence and calcium imaging analysis showed that mutant lamin changed NRVMs beating force and frequency. Additionally, we noted an altered microtubule network organization with shorter filament length, and defective hemichannel membrane localization (Connexin 43). These data highlight the interconnection between nucleoskeleton, cytoskeleton and sarcolemmal structures, and the transcellular consequences of mutant lamin protein in the pathogenesis of the cardiac laminopathies.
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- 2020
3. Biomechanical defects and rescue of cardiomyocytes expressing pathologic nuclear lamins
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Laurini, Erik, Martinelli, Valentina, Lanzicher, Thomas, Puzzi, Luca, Borin, Daniele, Chen, Suet Nee, Long, Carlin S, Lee, Patrice, Mestroni, Luisa, Taylor, Matthew RG, Sbaizero, Orfeo, and Pricl, Sabrina
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Biomedical and Clinical Sciences ,Cardiovascular Medicine and Haematology ,Cardiovascular ,Genetics ,Bioengineering ,Heart Disease ,5.1 Pharmaceuticals ,Development of treatments and therapeutic interventions ,Aetiology ,2.1 Biological and endogenous factors ,Animals ,Animals ,Newborn ,Biomechanical Phenomena ,Cardiomyopathies ,Cells ,Cultured ,Elastic Modulus ,Fluorescent Antibody Technique ,Genetic Predisposition to Disease ,Humans ,Lamin Type A ,Microscopy ,Atomic Force ,Molecular Dynamics Simulation ,Mutation ,Myocardial Contraction ,Myocytes ,Cardiac ,Phenotype ,Protein Conformation ,alpha-Helical ,Protein Kinase Inhibitors ,Rats ,Structure-Activity Relationship ,p38 Mitogen-Activated Protein Kinases ,Cardiorespiratory Medicine and Haematology ,Cardiovascular System & Hematology ,Cardiovascular medicine and haematology - Abstract
AimsGiven the clinical impact of LMNA cardiomyopathies, understanding lamin function will fulfill a clinical need and will lead to advancement in the treatment of heart failure. A multidisciplinary approach combining cell biology, atomic force microscopy (AFM), and molecular modeling was used to analyse the biomechanical properties of human lamin A/C gene (LMNA) mutations (E161K, D192G, N195K) using an in vitro neonatal rat ventricular myocyte model.Methods and resultsThe severity of biomechanical defects due to the three LMNA mutations correlated with the severity of the clinical phenotype. AFM and molecular modeling identified distinctive biomechanical and structural changes, with increasing severity from E161K to N195K and D192G, respectively. Additionally, the biomechanical defects were rescued with a p38 MAPK inhibitor.ConclusionsAFM and molecular modeling were able to quantify distinct biomechanical and structural defects in LMNA mutations E161K, D192G, and N195K and correlate the defects with clinical phenotypic severity. Improvements in cellular biomechanical phenotype was demonstrated and may represent a mechanism of action for p38 MAPK inhibition therapy that is now being used in human clinical trials to treat laminopathies.
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- 2018
4. Injectable Carbon Nanotube-Functionalized Reverse Thermal Gel Promotes Cardiomyocytes Survival and Maturation
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Peña, Brisa, Bosi, Susanna, Aguado, Brian A, Borin, Daniele, Farnsworth, Nikki L, Dobrinskikh, Evgenia, Rowland, Teisha J, Martinelli, Valentina, Jeong, Mark, Taylor, Matthew RG, Long, Carlin S, Shandas, Robin, Sbaizero, Orfeo, Prato, Maurizio, Anseth, Kristi S, Park, Daewon, and Mestroni, Luisa
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Engineering ,Nanotechnology ,Biomedical Engineering ,Bioengineering ,Cardiovascular ,Regenerative Medicine ,Heart Disease ,Animals ,Gelatin ,Myocytes ,Cardiac ,Nanotubes ,Carbon ,Rats ,Tissue Engineering ,Tissue Scaffolds ,carbon nanotube ,cardiac tissue engineering ,hybrid-biomimetic hydrogel ,injectable polymer ,reverse thermal gel ,Chemical Sciences ,Nanoscience & Nanotechnology ,Chemical sciences ,Physical sciences - Abstract
The ability of the adult heart to regenerate cardiomyocytes (CMs) lost after injury is limited, generating interest in developing efficient cell-based transplantation therapies. Rigid carbon nanotubes (CNTs) scaffolds have been used to improve CMs viability, proliferation, and maturation, but they require undesirable invasive surgeries for implantation. To overcome this limitation, we developed an injectable reverse thermal gel (RTG) functionalized with CNTs (RTG-CNT) that transitions from a solution at room temperature to a three-dimensional (3D) gel-based matrix shortly after reaching body temperature. Here we show experimental evidence that this 3D RTG-CNT system supports long-term CMs survival, promotes CMs alignment and proliferation, and improves CMs function when compared with traditional two-dimensional gelatin controls and 3D plain RTG system without CNTs. Therefore, our injectable RTG-CNT system could potentially be used as a minimally invasive tool for cardiac tissue engineering efforts.
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- 2017
5. EMID2 is a novel biotherapeutic for aggressive cancers identified by in vivo screening
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Cappelletto, Ambra, primary, Alfì, Edoardo, additional, Volf, Nina, additional, Bortolotti, Francesca, additional, Ciucci, Giulio, additional, Vodret, Simone, additional, Fantuz, Marco, additional, Perin, Martina, additional, Colliva, Andrea, additional, Rozzi, Giacomo, additional, Rossi, Matilde, additional, Ruozi, Giulia, additional, Zentilin, Lorena, additional, Vuerich, Roman, additional, Borin, Daniele, additional, Lapasin, Romano, additional, Piazza, Silvano, additional, Chiesa, Mattia, additional, Lorizio, Daniela, additional, Kumar, Sandeep, additional, Rustighi, Alessandra, additional, Jo, Hanjoong, additional, Piccolo, Stefano, additional, Carrer, Alessandro, additional, Giacca, Mauro, additional, Sal, Giannino Del, additional, and ZACCHIGNA, SERENA, additional
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- 2023
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6. Asbestos treatment technologies
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Paolini, Valerio, Tomassetti, Laura, Segreto, Marco, Borin, Daniele, Liotta, Flavia, Torre, Marco, and Petracchini, Francesco
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- 2019
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7. Cellular Biomechanic Impairment in Cardiomyocytes Carrying the Progeria Mutation: An Atomic Force Microscopy Investigation
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Peña, Brisa, primary, Gao, Shanshan, additional, Borin, Daniele, additional, Del Favero, Giorgia, additional, Abdel-Hafiz, Mostafa, additional, Farahzad, Nasim, additional, Lorenzon, Paola, additional, Sinagra, Gianfranco, additional, Taylor, Matthew R. G., additional, Mestroni, Luisa, additional, and Sbaizero, Orfeo, additional
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- 2022
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8. Actuation of silicon pillar micro-mechanical resonators by Kelvin polarization force
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Toffoli, Valeria, Dandash, Fadlallah, Pozzato, Alessandro, Borin, Daniele, Carrato, Sergio, and Lazzarino, Marco
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- 2013
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9. Production of lipid microparticles containing bioactive molecules functionalized with PEG
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Vezzù, Keti, Borin, Daniele, Bertucco, Alberto, Bersani, Sara, Salmaso, Stefano, and Caliceti, Paolo
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- 2010
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10. Viscoelastic behavior of cardiomyocytes carrying LMNA mutations
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Borin, Daniele, primary, Peña, Brisa, additional, Taylor, Matthew R.G., additional, Mestroni, Luisa, additional, Lapasin, Romano, additional, and Sbaizero, Orfeo, additional
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- 2020
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11. Knock Down of Plakophillin 2 Dysregulates Adhesion Pathway through Upregulation of miR200b and Alters the Mechanical Properties in Cardiac Cells
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Puzzi, Luca, primary, Borin, Daniele, additional, Gurha, Priyatansh, additional, Lombardi, Raffaella, additional, Martinelli, Valentina, additional, Weiss, Marek, additional, Andolfi, Laura, additional, Lazzarino, Marco, additional, Mestroni, Luisa, additional, Marian, Ali J., additional, and Sbaizero, Orfeo, additional
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- 2019
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12. Application of a dielectric barrier discharge plasma for heating plastic materials
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Borin, Daniele, primary, Sbaizero, Orfeo, additional, and Scuor, Nicola, additional
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- 2019
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13. Asbestos treatment technologies
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Paolini, Valerio, primary, Tomassetti, Laura, additional, Segreto, Marco, additional, Borin, Daniele, additional, Liotta, Flavia, additional, Torre, Marco, additional, and Petracchini, Francesco, additional
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- 2018
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14. Cellular biomechanics impairment in keratinocytes is associated with a C-terminal truncated desmoplakin: An atomic force microscopy investigation
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Puzzi, Luca, primary, Borin, Daniele, additional, Martinelli, Valentina, additional, Mestroni, Luisa, additional, Kelsell, David P., additional, and Sbaizero, Orfeo, additional
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- 2018
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15. Novel insights into cardiomyocytes provided by atomic force microscopy
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Borin, Daniele, primary, Pecorari, Ilaria, additional, Pena, Brisa, additional, and Sbaizero, Orfeo, additional
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- 2018
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16. An engineering insight into the relationship of selective cytoskeletal impairment and biomechanics of HeLa cells
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Borin, Daniele, primary, Puzzi, Luca, additional, Martinelli, Valentina, additional, Cibinel, Matteo, additional, Lapasin, Romano, additional, and Sbaizero, Orfeo, additional
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- 2017
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17. A Perspective on the Experimental Techniques for Studying Lamins
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Pecorari, Ilaria, primary, Borin, Daniele, additional, and Sbaizero, Orfeo, additional
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- 2017
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18. Micromechanical oscillators for biochemical applications
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Borin, Daniele, Dal Zilio, Simone, Scoles, Giacinto, and Lazzarino, Marco
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Liquid interaction ,MEMS ,FIS/01 FISICA SPERIMENTALE ,Micropillars ,Superhydrophobicity ,Biomarker detection ,SCUOLA DI DOTTORATO DI RICERCA IN NANOTECNOLOGIE - Abstract
2013/2014 Gli oscillatori micro e nanomeccanici stanno trovando crescente applicazione come biosensori, grazie alla loro elevata sensibilità, alla possibilità di effettuare analisi con un ridotto volume di campione biologico e senza molecole di marcatura secondarie e alla loro integrabilità come sensori in dispositivi di analisi portatili a basso costo. Questo progetto ha come scopo l’utilizzo di oscillatori micromeccanici a geometria verticale (micropillars) come sensori biomolecolari, rispetto ai più comuni oscillatori orizzontali (cantilevers). La struttura dei micropillars, infatti, permette di confinare l’adsorbimento molecolare alla parte superiore dell’oscillatore, consentendo una facile quantificazione della massa depositata attraverso la variazione della frequenza di risonanza; inoltre, i tempi di risposta del sensore risultano ridotti, grazie alla più veloce diffusione delle molecole verso l’area sensibile, rispetto ad altri sensori basati su effetti di superficie e di più grandi dimensioni. Dense matrici di micropillars idrofobici permettono anche la formazione dello stato superidrofobico di Cassie-Baxter, riducendo l’interazione del liquido alla sola parte superiore dell’oscillatore. Durante questo progetto è stato sviluppato un opportuno trattamento idrofobico delle matrici, basato sulla deposizione di un alcanosilano idrofobico, e caratterizzato rispetto alla bagnabilità della matrice e agli effetti sulla risposta meccanica e sulla risoluzione dell’oscillatore come sensore di massa. È stata inoltre verificata la compatibilità di questo trattamento con la formazione di uno strato di oro sulla parte superiore dell’oscillatore, usato come substrato per l’adsorbimento di biorecettori per il riconoscimento e la cattura della biomolecola di interesse. Il risultato di questa ottimizzazione è stato applicato alla rilevazione di un biomarcatore per il tumore alla prostata (PSMA) a concentrazioni utilizzate nella pratica clinica (nanomolari), sia in soluzione fisiologica che in plasma diluito. Infine, è stata dimostrata la misura della frequenza di risonanza dei micropillars a contatto con il liquido nello stato superidrofobico di Cassie-Baxter, come alternativa al problematico utilizzo dei cantilever completamente immersi in liquido, aprendo la strada alla rilevazione in tempo reale di biomolecole da campioni biologici. Micro and nanomechanical resonators are playing a growing role in biosensing due to their high sensitivity, the possibility of label-free biomolecular detection with a reduced amount of biological sample and their potential integration as sensing tool on low cost, point of care devices. This project focuses on the application of arrays of micropillars resonators for biomolecular sensing, exploiting the advantages coming from the vertical geometry respect to the most common micro and nanocantilever horizontal sensors. Indeed, the biomolecular adsorption can be confined to the micron sized top area of pillars, allowing an easy quantification of the deposited mass and reducing the response time of the resonator due to the faster diffusion on the sensitive area respect to larger, surface based sensors. In addition, the superhydrophobic Cassie-Baxter state can be obtained on dense, hydrophobic micropillars arrays, limiting the interaction with the analyte solution to the top area of pillars. A proper hydrophobization treatment of the arrays, based on the deposition of a hydrophobic alkanosilane coating, has been developed and characterized in terms of wettability of the arrays and of the effects on the mechanical performance and on the mass resolution of the resonators. The compatibility of this treatment with the formation of stable gold layer on top of pillars as a substrate for bioreceptor adsorption has been also verified. The optimized micropillars arrays have been used for the detection of PSMA (Prostate Specific Membrane Antigen) at diagnostically relevant concentrations (nM level) both from physiological solution and from diluted serum. Finally, by exploiting the superhydrophobic Cassie-Baxter state, the direct measurement of the resonance frequency of micropillars in a liquid environment has been demonstrated, as an alternative to the cumbersome application of micro and nanocantilevers on the same conditions, paving the way toward real-time, biomolecular detection from biological samples. XXVII Ciclo 1983
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- 2015
19. Parallel optical read-out of micromechanical pillars applied to prostate specific membrane antigen detection
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Tardivo, Martina, primary, Toffoli, Valeria, additional, Fracasso, Giulio, additional, Borin, Daniele, additional, Dal Zilio, Simone, additional, Colusso, Andrea, additional, Carrato, Sergio, additional, Scoles, Giacinto, additional, Meneghetti, Moreno, additional, Colombatti, Marco, additional, and Lazzarino, Marco, additional
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- 2015
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20. How to engineer superhydrophobic micromechanical sensors preserving mass resolution
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Borin, Daniele, primary, Melli, Mauro, additional, Dal Zilio, Simone, additional, Toffoli, Valeria, additional, Scoles, Giacinto, additional, Toffoli, Giuseppe, additional, and Lazzarino, Marco, additional
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- 2014
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21. Cellular Biomechanic Impairment in Cardiomyocytes Carrying the Progeria Mutation: An Atomic Force Microscopy Investigation
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Brisa Peña, Shanshan Gao, Daniele Borin, Giorgia Del Favero, Mostafa Abdel-Hafiz, Nasim Farahzad, Paola Lorenzon, Gianfranco Sinagra, Matthew R. G. Taylor, Luisa Mestroni, Orfeo Sbaizero, Peña, Brisa, Gao, Shanshan, Borin, Daniele, Del Favero, Giorgia, Abdel-Hafiz, Mostafa, Farahzad, Nasim, Lorenzon, Paola, Sinagra, Gianfranco, Taylor, Matthew R. G., Mestroni, Luisa, and Sbaizero, Orfeo
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Nuclear mechanic ,Cardiomyocytes ,Progeria syndrome ,Cardiomyopathy ,Atomic Force Microscopy ,Beating ,Connexin 43 ,Nuclear mechanics ,Surfaces and Interfaces ,Cardiomyocyte ,Fibroblasts ,Lamin Type A ,Microscopy, Atomic Force ,Condensed Matter Physics ,Rats ,Biomechanical Phenomena ,Progeria ,Mutation ,Electrochemistry ,Animals ,Myocytes, Cardiac ,General Materials Science ,Spectroscopy - Abstract
Given the clinical effect of progeria syndrome, understanding the cell mechanical behavior of this pathology could benefit the patient's treatment. Progeria patients show a point mutation in the lamin A/C gene (LMNA), which could change the cell's biomechanical properties. This paper reports a mechano-dynamic analysis of a progeria mutation (c.1824 C > T, p.Gly608Gly) in neonatal rat ventricular myocytes (NRVMs) using cell indentation by atomic force microscopy to measure alterations in beating force, frequency, and contractile amplitude of selected cells within cell clusters. Furthermore, we examined the beating rate variability using a time-domain method that produces a Poincaré plot because beat-to-beat changes can shed light on the causes of arrhythmias. Our data have been further related to our cell phenotype findings, using immunofluorescence and calcium transient analysis, showing that mutant NRVMs display changes in both beating force and frequency. These changes were associated with a decreased gap junction localization (Connexin 43) in the mutant NRVMs even in the presence of a stable cytoskeletal structure (microtubules and actin filaments) when compared with controls (wild type and non-treated cells). These data emphasize the kindred between nucleoskeleton (LMNA), cytoskeleton, and the sarcolemmal structures in NRVM with the progeria Gly608Gly mutation, prompting future mechanistic and therapeutic investigations.
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- 2022
22. Altered microtubule structure, hemichannel localization and beating activity in cardiomyocytes expressing pathologic nuclear lamin A/C
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Carlin S. Long, Matthew R.G. Taylor, Luisa Mestroni, Daniele Borin, Orfeo Sbaizero, Brisa Peña, Suet Nee Chen, Borin, Daniele, Pena, Brisa, Nee Chen, Suet, Long, Carlin S., Taylor, Matthew R. G., Mestroni, Luisa, and Sbaizero, Orfeo
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0301 basic medicine ,Biological science ,Cardiomyopathy ,Cell ,Biomechanic ,Cardiology ,Connexin ,Bioengineering ,Cardiomyocyte ,Cardiovascular ,Article ,LMNA ,03 medical and health sciences ,Atomic force microscopy ,0302 clinical medicine ,Calcium imaging ,Microtubule ,medicine ,2.1 Biological and endogenous factors ,Biomechanics ,lcsh:Social sciences (General) ,Aetiology ,Cytoskeleton ,lcsh:Science (General) ,Mechanical property ,Cardiomyocytes ,Lamin A/C ,Multidisciplinary ,Chemistry ,Membrane ,Biological sciences ,Beating ,Cx43 ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,Heart Disease ,Nuclear lamina ,lcsh:H1-99 ,030217 neurology & neurosurgery ,Lamin ,lcsh:Q1-390 - Abstract
Given the clinical effect of laminopathies, understanding lamin mechanical properties will benefit the treatment of heart failure. Here we report a mechano-dynamic study of LMNA mutations in neonatal rat ventricular myocytes (NRVM) using single cell spectroscopy with Atomic Force Microscopy (AFM) and measured changes in beating force, frequency and contractile amplitude of selected mutant-expressing cells within cell clusters. Furthermore, since beat-to-beat variations can provide clues on the origin of arrhythmias, we analyzed the beating rate variability using a time-domain method which provides a Poincaré plot. Data were further correlated to cell phenotypes. Immunofluorescence and calcium imaging analysis showed that mutant lamin changed NRVMs beating force and frequency. Additionally, we noted an altered microtubule network organization with shorter filament length, and defective hemichannel membrane localization (Connexin 43). These data highlight the interconnection between nucleoskeleton, cytoskeleton and sarcolemmal structures, and the transcellular consequences of mutant lamin protein in the pathogenesis of the cardiac laminopathies., Biological sciences; Cardiology; Biomechanics; Cytoskeleton; Mechanical property; Membrane; Lamin A/C; Cardiomyopathy; Atomic force microscopy; Cardiomyocytes; Beating; Cx43
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- 2020
23. Knock Down of Plakophillin 2 Dysregulates Adhesion Pathway through Upregulation of miR200b and Alters the Mechanical Properties in Cardiac Cells
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Priyatansh Gurha, Luisa Mestroni, Daniele Borin, Laura Andolfi, Luca Puzzi, Valentina Martinelli, Marek Weiss, Raffaella Lombardi, Orfeo Sbaizero, Ali J. Marian, Marco Lazzarino, Puzzi, Luca, Borin, Daniele, Gurha, Priyatansh, Lombardi, Raffaella, Martinelli, Valentina, Weiss, Marek, Andolfi, Laura, Lazzarino, Marco, Mestroni, Luisa, Marian, Ali J., Sbaizero, Orfeo, and Marian, Ali J
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0301 basic medicine ,Cell Plasticity ,arrhythmogenic cardiomyopathy ,PKP2 ,AFM ,microRNA ,cell adhesion ,intercalated disk ,focal adhesion ,Article ,Cell Line ,Focal adhesion ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Desmosome ,medicine ,Animals ,Myocytes, Cardiac ,Cytoskeleton ,Cell adhesion ,Actin ,Gene knockdown ,Chemistry ,Myocardium ,Desmosomes ,General Medicine ,Actin cytoskeleton ,Cell biology ,MicroRNAs ,030104 developmental biology ,medicine.anatomical_structure ,Plakophilins ,030217 neurology & neurosurgery - Abstract
Background: Mutations in genes encoding intercalated disk/desmosome proteins, such as plakophilin 2 (PKP2), cause arrhythmogenic cardiomyopathy (ACM). Desmosomes are responsible for myocyte&ndash, myocyte attachment and maintaining mechanical integrity of the myocardium. Methods: We knocked down Pkp2 in HL-1 mouse atrial cardiomyocytes (HL-1Pkp2-shRNA) and characterized their biomechanical properties. Gene expression was analyzed by RNA-Sequencing, microarray, and qPCR. Immunofluorescence was used to detect changes in cytoskeleton and focal adhesion. Antagomirs were used to knock down expression of selected microRNA (miR) in the rescue experiments. Results: Knockdown of Pkp2 was associated with decreased cardiomyocyte stiffness and work of detachment, and increased plasticity index. Altered mechanical properties were associated with impaired actin cytoskeleton in HL-1Pkp2-shRNA cells. Analysis of differentially expressed genes identified focal adhesion and actin cytoskeleton amongst the most dysregulated pathways, and miR200 family (a, b, and 429) as the most upregulated miRs in HL-1Pkp2-shRNA cells. Knockdown of miR-200b but not miR-200a, miR-429, by sequence-specific shRNAs partially rescued integrin-&alpha, 1 (Itga1) levels, actin organization, cell adhesion (on collagen), and stiffness. Conclusions: PKP2 deficiency alters cardiomyocytes adhesion through a mechanism that involves upregulation of miR-200b and suppression of Itga1 expression. These findings provide new insights into the molecular basis of altered mechanosensing in ACM.
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- 2019
24. Parallel optical read-out of micromechanical pillars applied to prostate specific membrane antigen detection
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Martina Tardivo, Sergio Carrato, Giulio Fracasso, Daniele Borin, Andrea Colusso, Moreno Meneghetti, Marco Lazzarino, Giacinto Scoles, Marco Colombatti, Valeria Toffoli, Simone Dal Zilio, Tardivo, Martina, Toffoli, Valeria, Fracasso, Giulio, Borin, Daniele, Dal Zilio, Simone, Colusso, Andrea, Carrato, Sergio, Scoles, Giacinto, Meneghetti, Moreno, Colombatti, Marco, and Lazzarino, Marco
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Male ,Analyte ,Materials science ,Biomedical Engineering ,Biophysics ,Frequency shift ,Nanotechnology ,Biosensing Techniques ,Tracking (particle physics) ,Buffer (optical fiber) ,parallel optical read-out detection ,Resonator ,Limit of Detection ,Biomarkers, Tumor ,PSMA ,Electrochemistry ,Glutamate carboxypeptidase II ,Humans ,Micromechanical sensors ,Parallel optical read-out detection ,Prostate specific membrane antigen ,Biotechnology ,Micromechanical sensors Parallel optical read-out detection Prostate Specific Membrane Antigen ,Prostate ,Prostatic Neoplasms ,Equipment Design ,General Medicine ,Micro-Electrical-Mechanical Systems ,Prostate-Specific Antigen ,biosensors ,prostate cancer ,Prostate Specific Membrane Antigen ,Antibodies, Immobilized ,Biosensor ,Sensitivity (electronics) ,Biomedical engineering - Abstract
Micro and nanomechanical resonators represent a promising platform for proteins label-free detection because of their extreme sensitivity, fast response and low cost. Micro-pillars are columnar resonators that can be easily arranged in dense arrays of several thousand sensors in a squared mm. To exploit such a large density, however, a method for tracking independently micropillars resonance frequency is required. Here we present a detection method based on CCD imaging and software image analysis, which can measure the resonance frequency of tens of pillars in parallel. Acquiring simultaneously the frequency shift of up to 40 sensors and applying a proper statistical analysis, we were able to overcome the variability of the single measures improving the device sensitivity at low analyte concentration range. As a proof of concept, this method has been tested for the detection of a tumor marker, the Prostate Specific Membrane Antigen (PSMA). Pillars have been functionalized with an antibody against PSMA. The tumor marker (PSMA) has been detected in a range of concentrations between 300 pM and 100 nM, in buffer and in diluted bovine serum. The sensitivity of our method was limited only by the affinity constant of the antigen–antibody recognition. Moreover, this detection technique demonstrated to be effective in the 1–6 nM range, which is the window of PSMA concentration of clinical interest.
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- 2015
25. A Perspective on the Experimental Techniques for Studying Lamins
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Daniele Borin, Orfeo Sbaizero, Ilaria Pecorari, Pecorari, Ilaria, Borin, Daniele, and Sbaizero, Orfeo
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0301 basic medicine ,spectroscopy ,General Medicine ,Computational biology ,Review ,Biology ,experimental techniques ,lamins ,mechanics ,microscopy ,mechanic ,03 medical and health sciences ,030104 developmental biology ,lcsh:Biology (General) ,embryonic structures ,Nuclear lamina ,lamin ,Intermediate filament ,lcsh:QH301-705.5 ,experimental technique ,Lamin - Abstract
Lamins are type V intermediate filaments that collectively form a meshwork underneath the inner nuclear membrane, called nuclear lamina. Furthermore, they are also present in the nucleoplasm. Lamins are experiencing a growing interest, since a wide range of diseases are induced by mutations in the gene coding for A-type lamins, globally known as laminopathies. Moreover, it has been demonstrated that lamins are involved in other pathological conditions, like cancer. The role of lamins has been studied from several perspectives, exploiting different techniques and procedures. This multidisciplinary approach has contributed to resolving the unique features of lamins and has provided a thorough insight in their role in living organisms. Yet, there are still many unanswered questions, which constantly generate research in the field. The present work is aimed to review some interesting experimental techniques performed so far to study lamins. Scientists can take advantage of this collection for their novel investigations, being aware of the already pursued and consolidated methodologies. Hopefully, advances in these research directions will provide insights to achieve better diagnostic procedures and effective therapeutic options.
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- 2017
26. Biomechanical defects and rescue of cardiomyocytes expressing pathologic nuclear lamins
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Valentina Martinelli, Suet Nee Chen, Thomas Lanzicher, Daniele Borin, Luisa Mestroni, Luca Puzzi, Erik Laurini, Sabrina Pricl, Matthew R.G. Taylor, Orfeo Sbaizero, Carlin S. Long, Patrice A Lee, Laurini, Erik, Martinelli, Valentina, Lanzicher, Thoma, Puzzi, Luca, Borin, Daniele, Chen, Suet Nee, Long, Carlin S, Lee, Patrice, Mestroni, Luisa, Taylor, Matthew R G, Sbaizero, Orfeo, and Pricl, Sabrina
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0301 basic medicine ,Protein Conformation, alpha-Helical ,Physiology ,Protein Conformation ,Fluorescent Antibody Technique ,cardiomyocyte ,cardiomyocytes ,Cardiorespiratory Medicine and Haematology ,medicine.disease_cause ,Cardiovascular ,Microscopy, Atomic Force ,p38 Mitogen-Activated Protein Kinases ,LMNA ,0302 clinical medicine ,Medicine ,Myocyte ,2.1 Biological and endogenous factors ,Myocytes, Cardiac ,Aetiology ,Cells, Cultured ,lamin A/C ,Mutation ,Microscopy ,Cultured ,Atomic Force ,Lamin Type A ,Phenotype ,Cell biology ,Biomechanical Phenomena ,Heart Disease ,5.1 Pharmaceuticals ,Nuclear lamina ,Development of treatments and therapeutic interventions ,AFM ,Cardiology and Cardiovascular Medicine ,Cardiomyopathies ,Cardiac ,Cells ,Bioengineering ,macromolecular substances ,cardiomyopathy ,cell physiology ,molecular modeling ,Molecular Dynamics Simulation ,03 medical and health sciences ,Structure-Activity Relationship ,Physiology (medical) ,Elastic Modulus ,Genetics ,Animals ,Humans ,Genetic Predisposition to Disease ,Protein Kinase Inhibitors ,Myocytes ,business.industry ,alpha-Helical ,Original Articles ,Newborn ,Myocardial Contraction ,Rats ,030104 developmental biology ,Cardiovascular System & Hematology ,Animals, Newborn ,business ,030217 neurology & neurosurgery ,Lamin ,Function (biology) - Abstract
Aims Given the clinical impact of LMNA cardiomyopathies, understanding lamin function will fulfill a clinical need and will lead to advancement in the treatment of heart failure. A multidisciplinary approach combining cell biology, atomic force microscopy (AFM), and molecular modeling was used to analyse the biomechanical properties of human lamin A/C gene (LMNA) mutations (E161K, D192G, N195K) using an in vitro neonatal rat ventricular myocyte model. Methods and results The severity of biomechanical defects due to the three LMNA mutations correlated with the severity of the clinical phenotype. AFM and molecular modeling identified distinctive biomechanical and structural changes, with increasing severity from E161K to N195K and D192G, respectively. Additionally, the biomechanical defects were rescued with a p38 MAPK inhibitor. Conclusions AFM and molecular modeling were able to quantify distinct biomechanical and structural defects in LMNA mutations E161K, D192G, and N195K and correlate the defects with clinical phenotypic severity. Improvements in cellular biomechanical phenotype was demonstrated and may represent a mechanism of action for p38 MAPK inhibition therapy that is now being used in human clinical trials to treat laminopathies.
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- 2017
27. An engineering insight into the relationship of selective cytoskeletal impairment and biomechanics of HeLa cells
- Author
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Orfeo Sbaizero, Romano Lapasin, Daniele Borin, Valentina Martinelli, Luca Puzzi, Matteo Cibinel, Borin, Daniele, Puzzi, L., Martinelli, V., Cibinel, Matteo, Lapasin, Romano, and Sbaizero, Orfeo
- Subjects
0301 basic medicine ,Materials science ,storage modulus ,General Physics and Astronomy ,Motility ,02 engineering and technology ,mechanical properties ,Microscopy, Atomic Force ,Microfilament ,Microtubules ,Viscoelasticity ,law.invention ,HeLa ,03 medical and health sciences ,Structural Biology ,Confocal microscopy ,law ,Microtubule ,Cell Line, Tumor ,Elastic Modulus ,mechanical propertie ,Humans ,General Materials Science ,Cytoskeleton ,Cell Shape ,Actin ,viscoelasticity ,Microscopy, Confocal ,AFM ,loss modulus ,Cell Biology ,Adhesion ,021001 nanoscience & nanotechnology ,Actins ,Elasticity ,Biomechanical Phenomena ,Cell biology ,030104 developmental biology ,0210 nano-technology ,storage modulu ,HeLa Cells - Abstract
It is widely accepted that the pathological state of cells is characterized by a modification of mechanical properties, affecting cellular shape and viscoelasticity as well as adhesion behaviour and motility. Thus, assessing these parameters could represent an interesting tool to monitor disease development and progression, but also the effects of drug treatments. Since biomechanical properties of cells are strongly related to cytoskeletal architecture, in this work we extensively studied the effects of selective impairments of actin microfilaments and microtubules on HeLa cells through force-deformation curves and stress relaxation tests with atomic force microscopy. Confocal microscopy was also used to display the effects of the used drugs on the cytoskeletal structure. In synergy with the aforementioned methods, stress relaxation data were used to assess the storage and loss moduli, as a complementary way to describe the influence of cytoskeletal components on cellular viscoelasticity. Our results indicate that F-actin and microtubules play a complementary role in the cell stiffness and viscoelasticity, and both are fundamental for the adhesion properties. Our data support also the application of biomechanics as a tool to study diseases and their treatments.
- Published
- 2017
28. Application of a dielectric barrier discharge plasma for heating plastic materials
- Author
-
Orfeo Sbaizero, Nicola Scuor, Daniele Borin, Borin, Daniele, Sbaizero, O, and Scuor, Nicola
- Subjects
Nuclear and High Energy Physics ,Materials science ,Nuclear Energy and Engineering ,DBD plasma ,heating ,plastics ,Plastic materials ,Dielectric barrier discharge ,Plasma ,Composite material ,Condensed Matter Physics - Abstract
Dielectric barrier discharge (DBD) plasma is usually referred in literature as a form of cold plasma. Many applications of DBD plasma rely on this characteristic, which allow to treat also sensitive materials, including biological tissues, to exploit a range of different effects. Atmospheric pressure plasma treatment is regularly used on polymers to enhance surface properties such as wettability and adhesion. However, in the present work, we show that DBD plasma can also be used as an alternative for heating polymeric based materials, as an initial step for further industrial processing such as thermo-forming. In particular, the efficiency of the heating process has been measured, and a novel heating mechanism has been proposed based on the experimental results
- Published
- 2019
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